Rh4CG171800

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005208
Physical Location & Seq
Reverse (-)
37601626 .. 37611367
9742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG171800.1

Sequence Viewer

Length: 633 bp
ATGCAGTTTAAATTTGTAGGAGTGACGTACTTCTCCAATATAAGGCGATTAATCGATGATTGCAAGGTTCTTGCAGCTGAGGTCAGTGTTGCCCTTCCGGCTCTGGATTCCCAAGACTCTGAGCACGCTAGCTGGTGCTGTTCCCGGATCGTCATCTCCGGTGACACAATTGAAGAGGAAGCGGTCGGCGAAGCTTTGCATTCCGATTGTATCGACGGGATTTGGGGCTGCTCCGGGGACGCCGTCTACGGCGGCGTGGAGAGAGGTGGTGGAAGATTGAGAAGGTTGCTTCTCCTCCTATGTGATTGGCCTTCCACCAAGGTCGATATTTCTGGGGTTAATGTGGAAAAATTTGATGACAAGTATTTCGGAAAGGCTGTGGAAAAGAAGAAGAAGAAGGGGGAGGGAGAGTTTTTTGAGGCAGAGAAAGAGGACAAGAAAGTGTTCCCACAAGAGAGGAAAAATGATCAGAAGAGTGTGGATGCTTCGTTGATAAAGTCCATTGACGCTGTCCCAGACTTGAAGACATACTTAGCTGCAAGATTCTCTCTGAGGAGGTGTAGAGAAACATATATCTCGTCTATGAGGGCATTTATCGCTATGAGCATAAATCTCGTCAAGGAGGTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L6e Ribosomal_L6e_N

Protein Analysis

210

Amino Acids

23.52

Weight (kDa)

5.85

Isoelectric Point (pI)

32.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L6e PF01159 105 - 186 4.5e-23 Ribosomal protein L6e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 246
AciI CCGC 2 cut(s) 182, 252
AclWI GGATC 1 cut(s) 155
AcsI RAATTY 2 cut(s) 11, 350
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 1 cut(s) 42
AgsI TTSAA 2 cut(s) 173, 523
AluBI AGCT 4 cut(s) 77, 132, 194, 536
AluI AGCT 4 cut(s) 77, 132, 194, 536
Alw21I GWGCWC 1 cut(s) 126
AlwI GGATC 1 cut(s) 155
AoxI GGCC 1 cut(s) 308
ApeKI GCWGC 3 cut(s) 74, 228, 536
ApoI RAATTY 2 cut(s) 11, 350
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AseI ATTAAT 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 443
AsuC2I CCSGG 2 cut(s) 145, 235
AsuHPI GGTGA 1 cut(s) 173
AsuNHI GCTAGC 1 cut(s) 128
BbsI GAAGAC 1 cut(s) 530
Bbv12I GWGCWC 1 cut(s) 126
BbvCI CCTCAGC 1 cut(s) 78
BbvI GCAGC 3 cut(s) 86, 215, 523
BceAI ACGGC 2 cut(s) 227, 265
BcgI CGANNNNNNTGC 2 cut(s) 595, 629
BclI TGATCA 1 cut(s) 466
BcnI CCSGG 2 cut(s) 145, 235
BfaI CTAG 1 cut(s) 129
BglI GCCNNNNNGGC 1 cut(s) 98
BisI GCNGC 4 cut(s) 75, 229, 253, 537
BlsI GCNGC 4 cut(s) 76, 230, 254, 538
Bme1390I CCNGG 2 cut(s) 145, 235
BmrFI CCNGG 2 cut(s) 145, 235
BmsI GCATC 1 cut(s) 472
BmtI GCTAGC 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 530
Bpu10I CCTNAGC 1 cut(s) 78
BpuMI CCSGG 2 cut(s) 145, 235
Bsa29I ATCGAT 1 cut(s) 54
BsaBI GATNNNNATC 1 cut(s) 152
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 2 cut(s) 234, 318
BsaWI WCCGGW 1 cut(s) 158
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse8I GATNNNNATC 1 cut(s) 152
BseCI ATCGAT 1 cut(s) 54
BseDI CCNNGG 2 cut(s) 234, 318
BseGI GGATG 1 cut(s) 487
BseJI GATNNNNATC 1 cut(s) 152
BseLI CCNNNNNNNGG 1 cut(s) 42
BseMII CTCAG 3 cut(s) 69, 111, 542
BseRI GAGGAG 2 cut(s) 284, 568
BseXI GCAGC 3 cut(s) 86, 215, 523
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 1 cut(s) 310
BshVI ATCGAT 1 cut(s) 54
BsiEI CGRYCG 1 cut(s) 186
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 4 cut(s) 98, 145, 159, 234
BslFI GGGAC 2 cut(s) 251, 497
BslI CCNNNNNNNGG 1 cut(s) 42
BsmFI GGGAC 2 cut(s) 251, 497
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 1 cut(s) 310
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 2 cut(s) 147, 466
BspACI CCGC 2 cut(s) 182, 252
BspANI GGCC 1 cut(s) 310
BspCNI CTCAG 3 cut(s) 70, 112, 543
BspDI ATCGAT 1 cut(s) 54
BspOI GCTAGC 1 cut(s) 132
BspPI GGATC 1 cut(s) 155
BssECI CCNNGG 2 cut(s) 234, 318
BssMI GATC 2 cut(s) 147, 466
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 318
Bst6I CTCTTC 2 cut(s) 168, 467
BstACI GRCGYC 1 cut(s) 240
BstC8I GCNNGC 2 cut(s) 126, 130
BstDEI CTNAG 4 cut(s) 78, 120, 532, 551
BstF5I GGATG 1 cut(s) 487
BstKTI GATC 2 cut(s) 150, 469
BstMBI GATC 2 cut(s) 147, 466
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 2 cut(s) 98, 596
BstSCI CCNGG 2 cut(s) 143, 233
BstV1I GCAGC 3 cut(s) 86, 215, 523
BstV2I GAAGAC 1 cut(s) 530
Bsu15I ATCGAT 1 cut(s) 54
BsuRI GGCC 1 cut(s) 310
BsuTUI ATCGAT 1 cut(s) 54
BtsCI GGATG 1 cut(s) 487
BtsIMutI CAGTG 1 cut(s) 91
Cac8I GCNNGC 2 cut(s) 126, 130
ClaI ATCGAT 1 cut(s) 54
CseI GACGC 2 cut(s) 248, 515
Csp6I GTAC 1 cut(s) 28
CviJI RGCY 8 cut(s) 77, 101, 132, 194, 228, 310, 377, 536
CviKI_1 RGCY 8 cut(s) 77, 101, 132, 194, 228, 310, 377, 536
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 4 cut(s) 78, 120, 532, 551
DpnI GATC 2 cut(s) 149, 468
DpnII GATC 2 cut(s) 147, 466
DraI TTTAAA 1 cut(s) 10
Eam1104I CTCTTC 2 cut(s) 168, 467
EarI CTCTTC 2 cut(s) 168, 467
Eco130I CCWWGG 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 318
FaiI YATR 8 cut(s) 41, 301, 529, 571, 573, 584, 602, 608
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FaqI GGGAC 2 cut(s) 251, 497
FbaI TGATCA 1 cut(s) 466
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 4 cut(s) 75, 229, 253, 537
FokI GGATG 1 cut(s) 494
Fsp4HI GCNGC 4 cut(s) 75, 229, 253, 537
FspBI CTAG 1 cut(s) 129
GluI GCNGC 4 cut(s) 75, 229, 253, 537
HaeIII GGCC 1 cut(s) 310
HapII CCGG 4 cut(s) 98, 145, 159, 234
HgaI GACGC 2 cut(s) 248, 515
Hin1I GRCGYC 1 cut(s) 240
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 3 cut(s) 107, 116, 543
HpaII CCGG 4 cut(s) 98, 145, 159, 234
HphI GGTGA 1 cut(s) 173
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 5 cut(s) 121, 205, 371, 471, 552
Hpy188III TCNNGA 1 cut(s) 104
Hpy8I GTNNAC 1 cut(s) 247
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 4 cut(s) 104, 276, 321, 391
HpyCH4IV ACGT 1 cut(s) 26
HpyCH4V TGCA 5 cut(s) 4, 63, 74, 199, 539
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 596
HpyF3I CTNAG 4 cut(s) 78, 120, 532, 551
HpySE526I ACGT 1 cut(s) 26
Hsp92I GRCGYC 1 cut(s) 240
Ksp22I TGATCA 1 cut(s) 466
Kzo9I GATC 2 cut(s) 147, 466
LmnI GCTCC 1 cut(s) 236
LpnPI CCDG 8 cut(s) 89, 111, 118, 158, 172, 247, 318, 528
Lsp1109I GCAGC 3 cut(s) 86, 215, 523
LweI GCATC 1 cut(s) 472
MaeI CTAG 1 cut(s) 129
MaeII ACGT 1 cut(s) 26
MaeIII GTNAC 2 cut(s) 22, 161
MalI GATC 2 cut(s) 149, 468
MboI GATC 2 cut(s) 147, 466
MboII GAAGA 7 cut(s) 185, 285, 400, 403, 406, 484, 535
MfeI CAATTG 1 cut(s) 168
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 3 cut(s) 11, 168, 350
MlyI GAGTC 1 cut(s) 110
MroXI GAANNNNTTC 1 cut(s) 443
MseI TTAA 3 cut(s) 9, 50, 339
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 4 cut(s) 98, 145, 159, 234
MspR9I CCNGG 2 cut(s) 145, 235
MunI CAATTG 1 cut(s) 168
Mva1269I GAATGC 1 cut(s) 199
MwoI GCNNNNNNNGC 2 cut(s) 98, 596
NciI CCSGG 2 cut(s) 145, 235
NdeII GATC 2 cut(s) 147, 466
NheI GCTAGC 1 cut(s) 128
NmuCI GTSAC 2 cut(s) 22, 161
PctI GAATGC 1 cut(s) 199
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 2 cut(s) 107, 543
PflFI GACNNNGTC 2 cut(s) 242, 509
PfoI TCCNGGA 1 cut(s) 143
PkrI GCNGC 4 cut(s) 76, 230, 254, 538
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
PshBI ATTAAT 1 cut(s) 50
PsyI GACNNNGTC 2 cut(s) 242, 509
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
SaqAI TTAA 3 cut(s) 9, 50, 339
SatI GCNGC 4 cut(s) 75, 229, 253, 537
Sau3AI GATC 2 cut(s) 147, 466
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 2 cut(s) 145, 235
SduI GDGCHC 1 cut(s) 126
SfaNI GCATC 1 cut(s) 472
Sse9I AATT 3 cut(s) 11, 168, 350
SsiI CCGC 2 cut(s) 182, 252
SspMI CTAG 1 cut(s) 129
StyD4I CCNGG 2 cut(s) 143, 233
StyI CCWWGG 1 cut(s) 318
TaiI ACGT 1 cut(s) 29
TaqI TCGA 3 cut(s) 54, 213, 324
TasI AATT 3 cut(s) 11, 168, 350
TauI GCSGC 1 cut(s) 255
TfiI GAWTC 2 cut(s) 107, 543
Tru1I TTAA 3 cut(s) 9, 50, 339
Tru9I TTAA 3 cut(s) 9, 50, 339
TscAI CASTG 1 cut(s) 91
TseFI GTSAC 2 cut(s) 22, 161
TseI GCWGC 3 cut(s) 74, 228, 536
Tsp45I GTSAC 2 cut(s) 22, 161
TspRI CASTG 1 cut(s) 91
Tth111I GACNNNGTC 2 cut(s) 242, 509
VspI ATTAAT 1 cut(s) 50
XapI RAATTY 2 cut(s) 11, 350
XmiI GTMKAC 1 cut(s) 246
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.