Rw4G001330

60s ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005208
Physical Location & Seq
Reverse (-)
2433676 .. 2436039
2364 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G001330.1

Sequence Viewer

Length: 642 bp
ATGGTGGTCAAAAAGCCACGCACTAAGAAAATACTAGTAAAAACTAAAAAGAACAGTAAAAGCCTTCAACATACCTTCTTAATTAATAATTATATGGTGGATGATACACATGATAGAAAGAGGTTTCTGTTAATTACACCCGAGACTGTGGTGATTATACTTGCTGGAAGGTTTAAGGGGAAGAAAGTTGTTTTCCTTAAGCAGCTTTCATCTGGGTTGCTTCTTCCATTCAAGATTAATTGTGTTCCTTTGAGACGTGTCGATATTTCTGGGGTGAATGTGGAAAAATTTGATGACAAGTATTTCGGAAAGGCCGTGGAAAAGAAGAAGAAGAAGGGGGAGGGAGAGTTTTTTGAGGCAGAAAAAGAGGACAAGAAAGTGTTCCCACAAGAGAGGAAAGATGATCAGAAGAGTGTGGATGCTTCATTGATAAAGTCCATTGACGTTGTCCCAGACTTGAAGACATACTTAGCTGCAAGATTCTCTCTGAGGTCTGGCATGTCTTTTAGACAGTCAGCTCTTTTCTTACTTAGGATACTTGTTCCCGTTCTTGTTTGTTTCTCTACTATGATTGTATGTTCTGTTCACATTGAGATCCGAGATTTTGTACTGATCGGCATTGCCTTGCAAGGGATGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L6e Ribosomal_L6e_N

Protein Analysis

213

Amino Acids

24.35

Weight (kDa)

9.9

Isoelectric Point (pI)

36.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L6e PF01159 86 - 169 2.7e-21 Ribosomal protein L6e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 589
AcsI RAATTY 1 cut(s) 287
AfaI GTAC 1 cut(s) 609
AfiI CCNNNNNNNGG 1 cut(s) 630
AflII CTTAAG 1 cut(s) 197
AflIII ACRYGT 1 cut(s) 256
AgsI TTSAA 3 cut(s) 68, 232, 460
AhlI ACTAGT 1 cut(s) 34
AjiI CACGTC 1 cut(s) 257
AluBI AGCT 3 cut(s) 205, 473, 518
AluI AGCT 3 cut(s) 205, 473, 518
Alw26I GTCTC 2 cut(s) 137, 247
AlwI GGATC 1 cut(s) 589
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 1 cut(s) 312
ApeKI GCWGC 2 cut(s) 202, 473
ApoI RAATTY 1 cut(s) 287
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AseI ATTAAT 2 cut(s) 84, 237
Asp700I GAANNNNTTC 1 cut(s) 380
AsuHPI GGTGA 2 cut(s) 163, 286
AvaI CYCGRG 1 cut(s) 140
BbsI GAAGAC 1 cut(s) 467
BbvI GCAGC 2 cut(s) 214, 460
BceAI ACGGC 1 cut(s) 299
BciVI GTATCC 1 cut(s) 528
BclI TGATCA 1 cut(s) 403
BcoDI GTCTC 2 cut(s) 137, 247
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 1 cut(s) 35
BfrI CTTAAG 1 cut(s) 197
BfuI GTATCC 1 cut(s) 528
BisI GCNGC 2 cut(s) 203, 474
BlsI GCNGC 2 cut(s) 204, 475
BmeT110I CYCGRG 1 cut(s) 140
BmgBI CACGTC 1 cut(s) 257
BmsI GCATC 1 cut(s) 409
BpiI GAAGAC 1 cut(s) 467
BsaJI CCNNGG 1 cut(s) 315
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bsc4I CCNNNNNNNGG 1 cut(s) 630
Bse3DI GCAATG 1 cut(s) 618
BseDI CCNNGG 1 cut(s) 315
BseGI GGATG 3 cut(s) 106, 424, 639
BseLI CCNNNNNNNGG 1 cut(s) 630
BseMI GCAATG 1 cut(s) 618
BseMII CTCAG 1 cut(s) 479
BseXI GCAGC 2 cut(s) 214, 460
BshFI GGCC 1 cut(s) 314
BsiHKCI CYCGRG 1 cut(s) 140
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 1 cut(s) 630
BsmAI GTCTC 2 cut(s) 137, 247
BsmBI CGTCTC 1 cut(s) 247
BsmFI GGGAC 1 cut(s) 434
BsnI GGCC 1 cut(s) 314
BsoBI CYCGRG 1 cut(s) 140
Bsp143I GATC 3 cut(s) 403, 594, 612
BspANI GGCC 1 cut(s) 314
BspCNI CTCAG 1 cut(s) 480
BspPI GGATC 1 cut(s) 589
BspTI CTTAAG 1 cut(s) 197
BsrDI GCAATG 1 cut(s) 618
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 3 cut(s) 403, 594, 612
Bst4CI ACNGT 3 cut(s) 56, 148, 513
Bst6I CTCTTC 1 cut(s) 404
BstAFI CTTAAG 1 cut(s) 197
BstDEI CTNAG 4 cut(s) 24, 469, 488, 530
BstDSI CCRYGG 1 cut(s) 315
BstF5I GGATG 3 cut(s) 106, 424, 639
BstKTI GATC 3 cut(s) 406, 597, 615
BstMAI GTCTC 2 cut(s) 137, 247
BstMBI GATC 3 cut(s) 403, 594, 612
BstNSI RCATGY 1 cut(s) 502
BstV1I GCAGC 2 cut(s) 214, 460
BstV2I GAAGAC 1 cut(s) 467
BstX2I RGATCY 1 cut(s) 594
BstYI RGATCY 1 cut(s) 594
BsuI GTATCC 1 cut(s) 528
BsuRI GGCC 1 cut(s) 314
BtgI CCRYGG 1 cut(s) 315
BtrI CACGTC 1 cut(s) 257
BtsCI GGATG 3 cut(s) 106, 424, 639
Csp6I GTAC 1 cut(s) 608
CviAII CATG 2 cut(s) 110, 499
CviJI RGCY 6 cut(s) 16, 63, 205, 314, 473, 518
CviKI_1 RGCY 6 cut(s) 16, 63, 205, 314, 473, 518
CviQI GTAC 1 cut(s) 608
DdeI CTNAG 4 cut(s) 24, 469, 488, 530
DpnI GATC 3 cut(s) 405, 596, 614
DpnII GATC 3 cut(s) 403, 594, 612
Eam1104I CTCTTC 1 cut(s) 404
EarI CTCTTC 1 cut(s) 404
Eco88I CYCGRG 1 cut(s) 140
Esp3I CGTCTC 1 cut(s) 247
FaeI CATG 2 cut(s) 113, 502
FalI AAGNNNNNCTT 2 cut(s) 452, 484
FaqI GGGAC 1 cut(s) 434
FatI CATG 2 cut(s) 109, 498
FbaI TGATCA 1 cut(s) 403
Fnu4HI GCNGC 2 cut(s) 203, 474
FokI GGATG 2 cut(s) 113, 431
Fsp4HI GCNGC 2 cut(s) 203, 474
FspBI CTAG 1 cut(s) 35
GluI GCNGC 2 cut(s) 203, 474
HaeIII GGCC 1 cut(s) 314
Hin1II CATG 2 cut(s) 113, 502
HinfI GANTC 1 cut(s) 480
HphI GGTGA 2 cut(s) 163, 286
Hpy166II GTNNAC 1 cut(s) 586
Hpy188I TCNGA 4 cut(s) 308, 408, 489, 599
Hpy188III TCNNGA 1 cut(s) 232
Hpy8I GTNNAC 1 cut(s) 586
HpyAV CCTTC 4 cut(s) 74, 85, 162, 328
HpyCH4III ACNGT 3 cut(s) 56, 148, 513
HpyCH4IV ACGT 2 cut(s) 256, 444
HpyCH4V TGCA 2 cut(s) 476, 628
HpyF3I CTNAG 4 cut(s) 24, 469, 488, 530
HpySE526I ACGT 2 cut(s) 256, 444
Hsp92II CATG 2 cut(s) 113, 502
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 3 cut(s) 403, 594, 612
LpnPI CCDG 5 cut(s) 150, 198, 255, 465, 480
Lsp1109I GCAGC 2 cut(s) 214, 460
LweI GCATC 1 cut(s) 409
MaeI CTAG 1 cut(s) 35
MaeII ACGT 2 cut(s) 256, 444
MalI GATC 3 cut(s) 405, 596, 614
MboI GATC 3 cut(s) 403, 594, 612
MboII GAAGA 7 cut(s) 193, 215, 337, 340, 343, 421, 472
MflI RGATCY 1 cut(s) 594
MluCI AATT 5 cut(s) 81, 88, 132, 238, 287
MnlI CCTC 6 cut(s) 114, 334, 349, 361, 387, 483
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 6 cut(s) 80, 84, 131, 174, 198, 237
MspCI CTTAAG 1 cut(s) 197
NdeII GATC 3 cut(s) 403, 594, 612
NlaIII CATG 2 cut(s) 113, 502
NspI RCATGY 1 cut(s) 502
PacI TTAATTAA 1 cut(s) 84
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 480
PflFI GACNNNGTC 1 cut(s) 446
PkrI GCNGC 2 cut(s) 204, 475
PshBI ATTAAT 2 cut(s) 84, 237
PsrI GAACNNNNNNTAC 2 cut(s) 567, 599
PsuI RGATCY 1 cut(s) 594
PsyI GACNNNGTC 1 cut(s) 446
RsaI GTAC 1 cut(s) 609
RsaNI GTAC 1 cut(s) 608
SaqAI TTAA 6 cut(s) 80, 84, 131, 174, 198, 237
SatI GCNGC 2 cut(s) 203, 474
Sau3AI GATC 3 cut(s) 403, 594, 612
SetI ASST 9 cut(s) 77, 125, 173, 207, 259, 447, 475, 494, 520
SfaNI GCATC 1 cut(s) 409
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SpeI ACTAGT 1 cut(s) 34
Sse9I AATT 5 cut(s) 81, 88, 132, 238, 287
SspMI CTAG 1 cut(s) 35
TaaI ACNGT 3 cut(s) 56, 148, 513
TaiI ACGT 2 cut(s) 259, 447
TaqI TCGA 1 cut(s) 261
TasI AATT 5 cut(s) 81, 88, 132, 238, 287
TatI WGTACW 1 cut(s) 607
TfiI GAWTC 1 cut(s) 480
Tru1I TTAA 6 cut(s) 80, 84, 131, 174, 198, 237
Tru9I TTAA 6 cut(s) 80, 84, 131, 174, 198, 237
TseI GCWGC 2 cut(s) 202, 473
TspDTI ATGAA 2 cut(s) 198, 414
Tth111I GACNNNGTC 1 cut(s) 446
Vha464I CTTAAG 1 cut(s) 197
VspI ATTAAT 2 cut(s) 84, 237
XapI RAATTY 1 cut(s) 287
XceI RCATGY 1 cut(s) 502
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.