RLG00000017951

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004256
Physical Location & Seq
Forward (+)
23521230 .. 23524719
3490 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017951

Sequence Viewer

Length: 534 bp
ATGGAGATTCACCATGGAGGCCGGTTCTATAGGTTGGGTAGTGGTAGTAGGCAGTGCAAGGGAGGTGAGGTGGTGTATGTTGATAGATTAGATCCTGATAAAATGTCATGGCCTGATTTGAACATTTATACTAAAGACTTGGGTTATAGAGAACCCCCAATTATGTACTGGTTTATGTTACCTAGAAGTATTCAAGGAGAAAGTTGGTTACCAATATGCGAAGACAAGGATGTGGAAGCTGAGCTAGAGGATGAGGGCGGGGGATCTCCTAAAGAGGTTGATCACCAGAAGTATATTGGCAACATGGTAGAAGACCTGGAAGAAGTGATTGATGATGGTCCTACTGTGACAAGCAATTATTGTGCAGAGATTTCAAGAGAGGCAAATGGAAAGCAAAACAAGGGAGCAGTATCTTGTAGAGGATTGCACCATGACTTCATCGCCTCTCTCCGCCTCTGCAAAATGCTGCATAGAACCTTGCTCTGCACCGATAGCTTTAATGTCACCATCGCCTCTGCAAATCTCTGCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

178

Amino Acids

20.06

Weight (kDa)

5.02

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1-like PF26130 2 - 78 2.2e-11 PB1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 258, 451
AclWI GGATC 2 cut(s) 86, 271
AfaI GTAC 1 cut(s) 167
AgsI TTSAA 3 cut(s) 121, 194, 375
AjnI CCWGG 1 cut(s) 315
AluBI AGCT 3 cut(s) 239, 244, 495
AluI AGCT 3 cut(s) 239, 244, 495
AlwI GGATC 2 cut(s) 86, 271
AoxI GGCC 2 cut(s) 19, 110
ApeKI GCWGC 1 cut(s) 466
AspS9I GGNCC 1 cut(s) 338
AsuHPI GGTGA 3 cut(s) 77, 275, 496
AvaII GGWCC 1 cut(s) 338
BbsI GAAGAC 2 cut(s) 228, 318
BbvI GCAGC 1 cut(s) 453
BccI CCATC 2 cut(s) 329, 515
BciT130I CCWGG 1 cut(s) 317
BclI TGATCA 1 cut(s) 280
BfaI CTAG 2 cut(s) 183, 245
BfmI CTRYAG 1 cut(s) 28
BisI GCNGC 1 cut(s) 467
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 1 cut(s) 468
Bme1390I CCNGG 1 cut(s) 317
Bme18I GGWCC 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 338
BmrFI CCNGG 1 cut(s) 317
BpiI GAAGAC 2 cut(s) 228, 318
Bpu1102I GCTNAGC 1 cut(s) 240
BsaJI CCNNGG 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bse118I RCCGGY 1 cut(s) 21
Bse1I ACTGG 1 cut(s) 173
BseBI CCWGG 1 cut(s) 317
BseDI CCNNGG 1 cut(s) 13
BseGI GGATG 2 cut(s) 235, 256
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 1 cut(s) 173
BseXI GCAGC 1 cut(s) 453
BsgI GTGCAG 2 cut(s) 384, 469
BshFI GGCC 2 cut(s) 21, 112
BsiSI CCGG 1 cut(s) 22
BsnI GGCC 2 cut(s) 21, 112
Bsp143I GATC 3 cut(s) 91, 263, 280
Bsp1720I GCTNAGC 1 cut(s) 240
Bsp19I CCATGG 1 cut(s) 13
BspACI CCGC 2 cut(s) 258, 451
BspANI GGCC 2 cut(s) 21, 112
BspCNI CTCAG 1 cut(s) 232
BspPI GGATC 2 cut(s) 86, 271
BsrFI RCCGGY 1 cut(s) 21
BsrI ACTGG 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 21
BssECI CCNNGG 1 cut(s) 13
BssMI GATC 3 cut(s) 91, 263, 280
BssT1I CCWWGG 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 317
Bst4CI ACNGT 1 cut(s) 346
BstDEI CTNAG 1 cut(s) 240
BstDSI CCRYGG 1 cut(s) 13
BstEII GGTNACC 1 cut(s) 207
BstF5I GGATG 2 cut(s) 235, 256
BstKTI GATC 3 cut(s) 94, 266, 283
BstMBI GATC 3 cut(s) 91, 263, 280
BstMWI GCNNNNNNNGC 1 cut(s) 492
BstNI CCWGG 1 cut(s) 317
BstPI GGTNACC 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 315
BstSFI CTRYAG 1 cut(s) 28
BstV1I GCAGC 1 cut(s) 453
BstV2I GAAGAC 2 cut(s) 228, 318
BstX2I RGATCY 2 cut(s) 91, 263
BstYI RGATCY 2 cut(s) 91, 263
BsuRI GGCC 2 cut(s) 21, 112
BtgI CCRYGG 1 cut(s) 13
BtgZI GCGATG 2 cut(s) 424, 493
BtsCI GGATG 2 cut(s) 235, 256
BtsI GCAGTG 1 cut(s) 59
BtsIMutI CAGTG 1 cut(s) 59
Cfr10I RCCGGY 1 cut(s) 21
Cfr13I GGNCC 1 cut(s) 338
Csp6I GTAC 1 cut(s) 166
CviAII CATG 4 cut(s) 14, 108, 304, 431
CviJI RGCY 5 cut(s) 21, 112, 239, 244, 495
CviKI_1 RGCY 5 cut(s) 21, 112, 239, 244, 495
CviQI GTAC 1 cut(s) 166
DdeI CTNAG 1 cut(s) 240
DpnI GATC 3 cut(s) 93, 265, 282
DpnII GATC 3 cut(s) 91, 263, 280
EciI GGCGGA 1 cut(s) 440
Eco130I CCWWGG 1 cut(s) 13
Eco47I GGWCC 1 cut(s) 338
Eco91I GGTNACC 1 cut(s) 207
EcoO65I GGTNACC 1 cut(s) 207
EcoRII CCWGG 1 cut(s) 315
EcoT14I CCWWGG 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 13
FaeI CATG 4 cut(s) 17, 111, 307, 434
FatI CATG 4 cut(s) 13, 107, 303, 430
FauI CCCGC 1 cut(s) 251
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 1 cut(s) 467
FokI GGATG 2 cut(s) 242, 263
Fsp4HI GCNGC 1 cut(s) 467
FspBI CTAG 2 cut(s) 183, 245
GluI GCNGC 1 cut(s) 467
HaeIII GGCC 2 cut(s) 21, 112
HapII CCGG 1 cut(s) 22
Hin1II CATG 4 cut(s) 17, 111, 307, 434
HinfI GANTC 1 cut(s) 7
HpaII CCGG 1 cut(s) 22
HphI GGTGA 3 cut(s) 77, 275, 496
Hpy188III TCNNGA 2 cut(s) 95, 375
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4V TGCA 7 cut(s) 57, 365, 427, 459, 469, 486, 518
HpyF10VI GCNNNNNNNGC 1 cut(s) 492
HpyF3I CTNAG 1 cut(s) 240
Hsp92II CATG 4 cut(s) 17, 111, 307, 434
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 3 cut(s) 91, 263, 280
LmnI GCTCC 1 cut(s) 404
LpnPI CCDG 8 cut(s) 35, 108, 126, 154, 299, 302, 329, 514
Lsp1109I GCAGC 1 cut(s) 453
MaeI CTAG 2 cut(s) 183, 245
MaeIII GTNAC 4 cut(s) 177, 207, 346, 502
MalI GATC 3 cut(s) 93, 265, 282
MboI GATC 3 cut(s) 91, 263, 280
MboII GAAGA 3 cut(s) 233, 323, 332
MflI RGATCY 2 cut(s) 91, 263
MluCI AATT 2 cut(s) 159, 355
MseI TTAA 1 cut(s) 498
MspI CCGG 1 cut(s) 22
MspR9I CCNGG 1 cut(s) 317
MvaI CCWGG 1 cut(s) 317
MwoI GCNNNNNNNGC 1 cut(s) 492
NcoI CCATGG 1 cut(s) 13
NdeII GATC 3 cut(s) 91, 263, 280
NlaIII CATG 4 cut(s) 17, 111, 307, 434
NmuCI GTSAC 2 cut(s) 346, 502
PfeI GAWTC 1 cut(s) 7
PkrI GCNGC 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 315
PspEI GGTNACC 1 cut(s) 207
PspGI CCWGG 1 cut(s) 315
PspPI GGNCC 1 cut(s) 338
PsuI RGATCY 2 cut(s) 91, 263
RsaI GTAC 1 cut(s) 167
RsaNI GTAC 1 cut(s) 166
SaqAI TTAA 1 cut(s) 498
SatI GCNGC 1 cut(s) 467
Sau3AI GATC 3 cut(s) 91, 263, 280
Sau96I GGNCC 1 cut(s) 338
ScrFI CCNGG 1 cut(s) 317
SfcI CTRYAG 1 cut(s) 28
SinI GGWCC 1 cut(s) 338
Sse9I AATT 2 cut(s) 159, 355
SsiI CCGC 2 cut(s) 258, 451
SspMI CTAG 2 cut(s) 183, 245
StyD4I CCNGG 1 cut(s) 315
StyI CCWWGG 1 cut(s) 13
TaaI ACNGT 1 cut(s) 346
TasI AATT 2 cut(s) 159, 355
TatI WGTACW 1 cut(s) 165
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TscAI CASTG 1 cut(s) 59
TseFI GTSAC 2 cut(s) 346, 502
TseI GCWGC 1 cut(s) 466
Tsp45I GTSAC 2 cut(s) 346, 502
TspDTI ATGAA 1 cut(s) 427
TspRI CASTG 1 cut(s) 59
VpaK11BI GGWCC 1 cut(s) 338
XcmI CCANNNNNNNNNTGG 2 cut(s) 165, 293
XspI CTAG 2 cut(s) 183, 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.