Rh4DG145700

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004256
Physical Location & Seq
Reverse (-)
29026092 .. 29037374
11283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG145700.1

Sequence Viewer

Length: 516 bp
ATGGGTAAAATTGCTGACAGATTACAAGTAATATCTGATAGAAAAGATAAGCTCGGTTTAGTATATAGTGATGATCCAGGTTATCCTCGGGCATCACAAAGGTTGCCGAGTTCATATGTGTTGGATGGACCTGTGGTTGGAAGGGATGAAGACAAGAGAAAGATTGTTGAGTTGTTGTTGAGAGATCATGAGCCTTGTTGTACAACCAATTTCCAAGTTTTGGCCATTGTTGGAATGCCGGGACTTGGCAAGACCACACTTGCAGGGCATGTGTTTAATGATAAGGAAATGGGGCTGTTCAATCCAAAGGTTGAAGCTCAAAATGTTAACCAAGTTAATAATTCTGATAATGTTCTAAATTCTGCAAATCATGTGGCAAACGAGAATGGCATGCCATATGAAAATGTTGATGTTGATACTATGATGCAGGAGGTAAACCGTCAAACACCCACACAACAATCTGAACCAGTTTATGTCCAAAGTGTGGCTTCATCTCAACCAGTGCATTTATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

171

Amino Acids

18.89

Weight (kDa)

5.14

Isoelectric Point (pI)

28.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 51 - 97 3.7e-07 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 220, 484
AclWI GGATC 1 cut(s) 68
AcoI YGGCCR 1 cut(s) 222
AcsI RAATTY 1 cut(s) 358
AfaI GTAC 1 cut(s) 202
AfiI CCNNNNNNNGG 4 cut(s) 137, 220, 245, 484
AgsI TTSAA 2 cut(s) 301, 314
AjnI CCWGG 1 cut(s) 76
AluBI AGCT 2 cut(s) 52, 317
AluI AGCT 2 cut(s) 52, 317
AlwI GGATC 1 cut(s) 68
Ama87I CYCGRG 1 cut(s) 87
AoxI GGCC 1 cut(s) 222
ApoI RAATTY 1 cut(s) 358
AspS9I GGNCC 1 cut(s) 128
AsuC2I CCSGG 1 cut(s) 240
AvaI CYCGRG 1 cut(s) 87
AvaII GGWCC 1 cut(s) 128
BalI TGGCCA 1 cut(s) 224
BbsI GAAGAC 1 cut(s) 156
BccI CCATC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 78
BcnI CCSGG 1 cut(s) 240
Bme1390I CCNGG 2 cut(s) 78, 240
Bme18I GGWCC 1 cut(s) 128
BmeT110I CYCGRG 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 128
BmrFI CCNGG 2 cut(s) 78, 240
BmsI GCATC 2 cut(s) 101, 414
BpiI GAAGAC 1 cut(s) 156
BpuMI CCSGG 1 cut(s) 240
BsaJI CCNNGG 1 cut(s) 86
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 220, 245, 484
Bse1I ACTGG 2 cut(s) 467, 500
BseBI CCWGG 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 86
BseGI GGATG 2 cut(s) 130, 151
BseLI CCNNNNNNNGG 4 cut(s) 137, 220, 245, 484
BseNI ACTGG 2 cut(s) 467, 500
BshFI GGCC 1 cut(s) 224
BsiHKCI CYCGRG 1 cut(s) 87
BsiSI CCGG 1 cut(s) 239
BslFI GGGAC 1 cut(s) 255
BslI CCNNNNNNNGG 4 cut(s) 137, 220, 245, 484
BsmFI GGGAC 1 cut(s) 255
BsmI GAATGC 1 cut(s) 240
BsnI GGCC 1 cut(s) 224
BsoBI CYCGRG 1 cut(s) 87
Bsp1407I TGTACA 1 cut(s) 200
Bsp143I GATC 2 cut(s) 73, 184
BspANI GGCC 1 cut(s) 224
BspHI TCATGA 1 cut(s) 187
BspPI GGATC 1 cut(s) 68
BsrGI TGTACA 1 cut(s) 200
BsrI ACTGG 2 cut(s) 467, 500
BssECI CCNNGG 1 cut(s) 86
BssMI GATC 2 cut(s) 73, 184
Bst2UI CCWGG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 440
BstAUI TGTACA 1 cut(s) 200
BstC8I GCNNGC 1 cut(s) 392
BstF5I GGATG 2 cut(s) 130, 151
BstKTI GATC 2 cut(s) 76, 187
BstMBI GATC 2 cut(s) 73, 184
BstNI CCWGG 1 cut(s) 78
BstNSI RCATGY 2 cut(s) 272, 394
BstSCI CCNGG 2 cut(s) 76, 238
BstV2I GAAGAC 1 cut(s) 156
BsuRI GGCC 1 cut(s) 224
BtsCI GGATG 2 cut(s) 130, 151
BtsIMutI CAGTG 1 cut(s) 507
Cac8I GCNNGC 1 cut(s) 392
CciI TCATGA 1 cut(s) 187
Cfr13I GGNCC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 201
CspCI CAANNNNNGTGG 2 cut(s) 354, 389
CviAII CATG 4 cut(s) 188, 269, 371, 391
CviJI RGCY 6 cut(s) 52, 193, 224, 295, 317, 488
CviKI_1 RGCY 6 cut(s) 52, 193, 224, 295, 317, 488
CviQI GTAC 1 cut(s) 201
DpnI GATC 2 cut(s) 75, 186
DpnII GATC 2 cut(s) 73, 184
EaeI YGGCCR 1 cut(s) 222
Eco47I GGWCC 1 cut(s) 128
Eco88I CYCGRG 1 cut(s) 87
EcoRII CCWGG 1 cut(s) 76
FaeI CATG 4 cut(s) 191, 272, 374, 394
FalI AAGNNNNNCTT 2 cut(s) 472, 504
FaqI GGGAC 1 cut(s) 255
FatI CATG 4 cut(s) 187, 268, 370, 390
FauNDI CATATG 2 cut(s) 115, 397
FokI GGATG 2 cut(s) 137, 158
HaeIII GGCC 1 cut(s) 224
HapII CCGG 1 cut(s) 239
Hin1II CATG 4 cut(s) 191, 272, 374, 394
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HpaI GTTAAC 1 cut(s) 328
HpaII CCGG 1 cut(s) 239
Hpy166II GTNNAC 2 cut(s) 328, 436
Hpy188I TCNGA 3 cut(s) 37, 346, 463
Hpy188III TCNNGA 1 cut(s) 188
Hpy8I GTNNAC 2 cut(s) 328, 436
HpyAV CCTTC 1 cut(s) 135
HpyCH4III ACNGT 1 cut(s) 440
HpyCH4V TGCA 4 cut(s) 263, 365, 427, 505
Hsp92II CATG 4 cut(s) 191, 272, 374, 394
KspAI GTTAAC 1 cut(s) 328
Kzo9I GATC 2 cut(s) 73, 184
LpnPI CCDG 7 cut(s) 63, 90, 144, 249, 252, 413, 480
LweI GCATC 2 cut(s) 101, 414
MalI GATC 2 cut(s) 75, 186
MboI GATC 2 cut(s) 73, 184
MboII GAAGA 1 cut(s) 161
MlsI TGGCCA 1 cut(s) 224
MluCI AATT 4 cut(s) 9, 208, 340, 358
MluNI TGGCCA 1 cut(s) 224
MmeI TCCRAC 3 cut(s) 102, 118, 211
MnlI CCTC 2 cut(s) 96, 424
Mox20I TGGCCA 1 cut(s) 224
MscI TGGCCA 1 cut(s) 224
MseI TTAA 3 cut(s) 276, 327, 336
Msp20I TGGCCA 1 cut(s) 224
MspI CCGG 1 cut(s) 239
MspR9I CCNGG 2 cut(s) 78, 240
Mva1269I GAATGC 1 cut(s) 240
MvaI CCWGG 1 cut(s) 78
NciI CCSGG 1 cut(s) 240
NdeI CATATG 2 cut(s) 115, 397
NdeII GATC 2 cut(s) 73, 184
NlaIII CATG 4 cut(s) 191, 272, 374, 394
NmeAIII GCCGAG 1 cut(s) 132
NspI RCATGY 2 cut(s) 272, 394
PaeI GCATGC 1 cut(s) 394
PagI TCATGA 1 cut(s) 187
PctI GAATGC 1 cut(s) 240
PflMI CCANNNNNTGG 2 cut(s) 220, 484
Psp6I CCWGG 1 cut(s) 76
PspGI CCWGG 1 cut(s) 76
PspPI GGNCC 1 cut(s) 128
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
SaqAI TTAA 3 cut(s) 276, 327, 336
Sau3AI GATC 2 cut(s) 73, 184
Sau96I GGNCC 1 cut(s) 128
ScrFI CCNGG 2 cut(s) 78, 240
SetI ASST 7 cut(s) 54, 82, 104, 133, 312, 319, 435
SfaNI GCATC 2 cut(s) 101, 414
SinI GGWCC 1 cut(s) 128
SphI GCATGC 1 cut(s) 394
Sse9I AATT 4 cut(s) 9, 208, 340, 358
StyD4I CCNGG 2 cut(s) 76, 238
TaaI ACNGT 1 cut(s) 440
TasI AATT 4 cut(s) 9, 208, 340, 358
TatI WGTACW 1 cut(s) 200
Tru1I TTAA 3 cut(s) 276, 327, 336
Tru9I TTAA 3 cut(s) 276, 327, 336
TscAI CASTG 1 cut(s) 507
TspDTI ATGAA 4 cut(s) 102, 162, 414, 480
TspRI CASTG 1 cut(s) 507
Van91I CCANNNNNTGG 2 cut(s) 220, 484
VpaK11BI GGWCC 1 cut(s) 128
XapI RAATTY 1 cut(s) 358
XceI RCATGY 2 cut(s) 272, 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.