RLG00000020445

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001527
Physical Location & Seq
Reverse (-)
65117170 .. 65119353
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020445

Sequence Viewer

Length: 603 bp
ATGTCCACAATTGCAACTAAAGTTTGGGCTAGAATGTTATACAACAAAAGCTTAGTAACCAAATTCTCAACAAGGCAACTACTGACGGACTTGATCATCCCGCCGATAAAGCCCTCAACCCCTCACCATTCGAAGCCGCGGCGACCTCAACTCGAGATGGGTGAAGCAGTCAAGGTAGTGGTTCCAGAGTCTGTCCTAAAGAAGAGGAAGAGAGAGGAGGAATGGGCTTTGGCAAAGAAGCAGGGGCTAGAAGCTGCCAAGAAGAAGAATGCTGAGAATAGGAAGCTTATTTACAACAAATCCAAACAGTACACCAAGGAGTACGCAGAGAAGCGTGAAGCAAGGCTGAAAGGAGGATTCTATATATTCAATGGTGTCTTCCTGAAAGGTTTGGGCAAGCATGGCATTATCTGCACTGAGGATCTTATTCACGAGATCTTGACAGTTGGACCTCATTTTAAGGAGGCCAACAACTTTCTGTGGCCATTTAAACTCAAGGCACCATTGGGTGGTCTTAAGAAGAAGAGGAACCATTATGTTGAAGGTGGAGATGCTGGAAACCGTGAGAACTACATCAATGAGCTTATTAGGAGAATGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L30 Ribosomal_L30_N

Protein Analysis

201

Amino Acids

23.07

Weight (kDa)

10.07

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L30_N PF08079 61 - 121 3.9e-11 Ribosomal L30 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 499
AccB7I CCANNNNNTGG 1 cut(s) 509
AccII CGCG 1 cut(s) 139
AciI CCGC 3 cut(s) 101, 137, 139
AclWI GGATC 1 cut(s) 429
AcoI YGGCCR 1 cut(s) 482
AcsI RAATTY 1 cut(s) 62
AfaI GTAC 2 cut(s) 311, 323
AfiI CCNNNNNNNGG 1 cut(s) 509
AflII CTTAAG 1 cut(s) 515
AgsI TTSAA 2 cut(s) 370, 542
AluBI AGCT 4 cut(s) 51, 254, 286, 583
AluI AGCT 4 cut(s) 51, 254, 286, 583
AlwI GGATC 1 cut(s) 429
AlwNI CAGNNNCTG 1 cut(s) 191
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 2 cut(s) 465, 482
ApeKI GCWGC 1 cut(s) 254
ApoI RAATTY 1 cut(s) 62
AspS9I GGNCC 1 cut(s) 449
AsuHPI GGTGA 2 cut(s) 116, 173
AsuII TTCGAA 1 cut(s) 131
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 1 cut(s) 449
BalI TGGCCA 1 cut(s) 484
BanI GGYRCC 1 cut(s) 499
BarI GAAGNNNNNNTAC 2 cut(s) 275, 307
BauI CACGAG 1 cut(s) 431
BbsI GAAGAC 1 cut(s) 370
BbvI GCAGC 1 cut(s) 241
BccI CCATC 1 cut(s) 151
BclI TGATCA 1 cut(s) 93
BfaI CTAG 3 cut(s) 30, 248, 601
BfrI CTTAAG 1 cut(s) 515
BglII AGATCT 1 cut(s) 435
BisI GCNGC 3 cut(s) 137, 140, 255
BlsI GCNGC 3 cut(s) 138, 141, 256
Bme18I GGWCC 1 cut(s) 449
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 1 cut(s) 449
BmiI GGNNCC 3 cut(s) 183, 501, 530
BmsI GCATC 1 cut(s) 541
BpiI GAAGAC 1 cut(s) 370
Bpu14I TTCGAA 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 479
BsaJI CCNNGG 2 cut(s) 137, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 509
BseDI CCNNGG 2 cut(s) 137, 315
BseGI GGATG 1 cut(s) 96
BseLI CCNNNNNNNGG 1 cut(s) 509
BseMII CTCAG 2 cut(s) 264, 408
BseRI GAGGAG 1 cut(s) 230
BseXI GCAGC 1 cut(s) 241
BsgI GTGCAG 1 cut(s) 397
Bsh1236I CGCG 1 cut(s) 139
BshFI GGCC 2 cut(s) 467, 484
BshNI GGYRCC 1 cut(s) 499
BsiHKCI CYCGRG 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 509
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 2 cut(s) 467, 484
BsoBI CYCGRG 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 131
Bsp143I GATC 3 cut(s) 93, 421, 435
BspACI CCGC 3 cut(s) 101, 137, 139
BspANI GGCC 2 cut(s) 467, 484
BspCNI CTCAG 2 cut(s) 265, 409
BspFNI CGCG 1 cut(s) 139
BspLI GGNNCC 3 cut(s) 183, 501, 530
BspPI GGATC 1 cut(s) 429
BspT104I TTCGAA 1 cut(s) 131
BspT107I GGYRCC 1 cut(s) 499
BspTI CTTAAG 1 cut(s) 515
BssECI CCNNGG 2 cut(s) 137, 315
BssMI GATC 3 cut(s) 93, 421, 435
BssSI CACGAG 1 cut(s) 431
BssT1I CCWWGG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 431
Bst4CI ACNGT 3 cut(s) 309, 445, 563
Bst6I CTCTTC 3 cut(s) 197, 203, 518
BstAFI CTTAAG 1 cut(s) 515
BstAPI GCANNNNNTGC 1 cut(s) 411
BstBI TTCGAA 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 398
BstDEI CTNAG 3 cut(s) 52, 273, 417
BstDSI CCRYGG 1 cut(s) 137
BstF5I GGATG 1 cut(s) 96
BstFNI CGCG 1 cut(s) 139
BstKTI GATC 3 cut(s) 96, 424, 438
BstMBI GATC 3 cut(s) 93, 421, 435
BstMWI GCNNNNNNNGC 3 cut(s) 109, 402, 411
BstUI CGCG 1 cut(s) 139
BstV1I GCAGC 1 cut(s) 241
BstV2I GAAGAC 1 cut(s) 370
BstX2I RGATCY 2 cut(s) 421, 435
BstYI RGATCY 2 cut(s) 421, 435
BsuRI GGCC 2 cut(s) 467, 484
BtgI CCRYGG 1 cut(s) 137
BtsCI GGATG 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 414
Cac8I GCNNGC 1 cut(s) 398
CaiI CAGNNNCTG 1 cut(s) 191
Cfr13I GGNCC 1 cut(s) 449
Cfr42I CCGCGG 1 cut(s) 140
Csp6I GTAC 2 cut(s) 310, 322
CviAII CATG 1 cut(s) 401
CviQI GTAC 2 cut(s) 310, 322
DdeI CTNAG 3 cut(s) 52, 273, 417
DpnI GATC 3 cut(s) 95, 423, 437
DpnII GATC 3 cut(s) 93, 421, 435
DraI TTTAAA 1 cut(s) 490
EaeI YGGCCR 1 cut(s) 482
Eam1104I CTCTTC 3 cut(s) 197, 203, 518
EarI CTCTTC 3 cut(s) 197, 203, 518
Eco130I CCWWGG 1 cut(s) 315
Eco47I GGWCC 1 cut(s) 449
Eco88I CYCGRG 1 cut(s) 152
EcoT14I CCWWGG 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 1 cut(s) 404
FaiI YATR 5 cut(s) 40, 363, 365, 402, 537
FatI CATG 1 cut(s) 400
FauI CCCGC 1 cut(s) 108
FbaI TGATCA 1 cut(s) 93
Fnu4HI GCNGC 3 cut(s) 137, 140, 255
FokI GGATG 1 cut(s) 83
Fsp4HI GCNGC 3 cut(s) 137, 140, 255
FspBI CTAG 3 cut(s) 30, 248, 601
GluI GCNGC 3 cut(s) 137, 140, 255
HaeIII GGCC 2 cut(s) 467, 484
Hin1II CATG 1 cut(s) 404
HindIII AAGCTT 2 cut(s) 49, 284
HinfI GANTC 2 cut(s) 188, 357
HphI GGTGA 2 cut(s) 116, 173
Hpy166II GTNNAC 2 cut(s) 6, 312
Hpy188III TCNNGA 5 cut(s) 154, 185, 382, 431, 439
Hpy8I GTNNAC 2 cut(s) 6, 312
HpyAV CCTTC 1 cut(s) 536
HpyCH4III ACNGT 3 cut(s) 309, 445, 563
HpyCH4V TGCA 2 cut(s) 14, 414
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 402, 411
HpyF3I CTNAG 3 cut(s) 52, 273, 417
Hsp92II CATG 1 cut(s) 404
Ksp22I TGATCA 1 cut(s) 93
KspI CCGCGG 1 cut(s) 140
Kzo9I GATC 3 cut(s) 93, 421, 435
LpnPI CCDG 4 cut(s) 198, 227, 395, 540
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 1 cut(s) 541
MaeI CTAG 3 cut(s) 30, 248, 601
MaeIII GTNAC 1 cut(s) 55
MalI GATC 3 cut(s) 95, 423, 437
MboI GATC 3 cut(s) 93, 421, 435
MboII GAAGA 7 cut(s) 214, 220, 274, 277, 370, 532, 535
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 2 cut(s) 421, 435
MlsI TGGCCA 1 cut(s) 484
MluCI AATT 2 cut(s) 9, 62
MluNI TGGCCA 1 cut(s) 484
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 427
Mox20I TGGCCA 1 cut(s) 484
MscI TGGCCA 1 cut(s) 484
MseI TTAA 3 cut(s) 459, 489, 516
Msp20I TGGCCA 1 cut(s) 484
MspA1I CMGCKG 1 cut(s) 139
MspCI CTTAAG 1 cut(s) 515
MunI CAATTG 1 cut(s) 9
Mva1269I GAATGC 1 cut(s) 274
MvnI CGCG 1 cut(s) 139
MwoI GCNNNNNNNGC 3 cut(s) 109, 402, 411
NdeII GATC 3 cut(s) 93, 421, 435
NlaIII CATG 1 cut(s) 404
NlaIV GGNNCC 3 cut(s) 183, 501, 530
NspV TTCGAA 1 cut(s) 131
PaeR7I CTCGAG 1 cut(s) 152
PctI GAATGC 1 cut(s) 274
PfeI GAWTC 1 cut(s) 357
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 3 cut(s) 138, 141, 256
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
PspN4I GGNNCC 3 cut(s) 183, 501, 530
PspPI GGNCC 1 cut(s) 449
PstNI CAGNNNCTG 1 cut(s) 191
PsuI RGATCY 2 cut(s) 421, 435
RsaI GTAC 2 cut(s) 311, 323
RsaNI GTAC 2 cut(s) 310, 322
SacII CCGCGG 1 cut(s) 140
SaqAI TTAA 3 cut(s) 459, 489, 516
SatI GCNGC 3 cut(s) 137, 140, 255
Sau3AI GATC 3 cut(s) 93, 421, 435
Sau96I GGNCC 1 cut(s) 449
SchI GAGTC 1 cut(s) 197
SetI ASST 9 cut(s) 53, 148, 177, 256, 288, 391, 454, 547, 585
SfaNI GCATC 1 cut(s) 541
Sfr274I CTCGAG 1 cut(s) 152
Sfr303I CCGCGG 1 cut(s) 140
SfuI TTCGAA 1 cut(s) 131
SgrBI CCGCGG 1 cut(s) 140
SinI GGWCC 1 cut(s) 449
SlaI CTCGAG 1 cut(s) 152
SmlI CTYRAG 3 cut(s) 152, 494, 515
SmoI CTYRAG 3 cut(s) 152, 494, 515
Sse9I AATT 2 cut(s) 9, 62
SsiI CCGC 3 cut(s) 101, 137, 139
SspMI CTAG 3 cut(s) 30, 248, 601
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 3 cut(s) 309, 445, 563
TaqI TCGA 2 cut(s) 131, 153
TasI AATT 2 cut(s) 9, 62
TatI WGTACW 1 cut(s) 309
TauI GCSGC 2 cut(s) 139, 142
TfiI GAWTC 1 cut(s) 357
Tru1I TTAA 3 cut(s) 459, 489, 516
Tru9I TTAA 3 cut(s) 459, 489, 516
TscAI CASTG 1 cut(s) 421
TseI GCWGC 1 cut(s) 254
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 1 cut(s) 421
Van91I CCANNNNNTGG 1 cut(s) 509
Vha464I CTTAAG 1 cut(s) 515
VpaK11BI GGWCC 1 cut(s) 449
XapI RAATTY 1 cut(s) 62
XhoI CTCGAG 1 cut(s) 152
XspI CTAG 3 cut(s) 30, 248, 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.