Rorug02G0416500

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001527
Physical Location & Seq
Reverse (-)
53420627 .. 53423497
2871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0416500.1

Sequence Viewer

Length: 759 bp
ATGGCCACGCCGCCGATGCCGCCGCCGAACATGGAGGGTGGCCCGCCGCAAGTTGTAGCACAGCCGCCGGGGACGGACATGACCGGAATATGCTTCAAGGACCAGCTTTGGCTGAGCTCTTATCCTCTCGATCGCAATCTGGTCTTTGATTACTTTGCAATCTCGCCCTTCTACGACTGGACCTGTAACAATGAACAGCTCCGTCAGCGCGCAATTCACCCTCTTGATATCTCTCACCTCTCCAAAATGACGGGGACAGAATACATGCTGACGGAAGTTACGGAGCCGCACCTGTTTGTCATCCGCAAGCAAAAGAGGGACAGTCCGGAGAAAGTAACACCCATGATGACTTACTATGTCTTGGATGGTTCGATATATCAAGCACCTCAGCTTTGCAATGTTTTTGCAGCTCGAGTTGGACGTGCTCTCTATCATATAAATAAGGCTTTCACCACTGCATCTTCAAACTTGGAGAAGATTGGATATGTTGATCCAGAAAATGAAACTGCAGCCTCTGAATCCAAGGCTAGCAAAGAGACAATTGACGTAAGGGAATTTAATCGAGTGAACCAAATTCTTCAATCGTTATTGCGCAAGCTACCACCAGCCCCACCACCGCCACCATTCCCAGAAGGCTATGTTCCTGTACCCACTGGAGAAGCCGAGAACGGCTCAGAAACTCAGCAAGCCGGAGAGCCACAACAACCTCCGGTGGATCCCATCATTGATCAAGGGCCAGCTAAAAGGATGAAATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L30 Ribosomal_L30_N

Protein Analysis

252

Amino Acids

28.22

Weight (kDa)

5.31

Isoelectric Point (pI)

61.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med6 PF04934 30 - 155 2.3e-43 MED6 mediator sub complex component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 593
AccII CGCG 1 cut(s) 210
AccIII TCCGGA 1 cut(s) 325
AciI CCGC 9 cut(s) 11, 20, 23, 44, 47, 65, 287, 304, 617
AclWI GGATC 3 cut(s) 485, 710, 723
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 554, 573
AfaI GTAC 1 cut(s) 648
AgsI TTSAA 3 cut(s) 97, 465, 581
AjiI CACGTC 1 cut(s) 422
AluBI AGCT 7 cut(s) 106, 117, 199, 391, 410, 598, 740
AluI AGCT 7 cut(s) 106, 117, 199, 391, 410, 598, 740
Alw21I GWGCWC 2 cut(s) 119, 427
Alw26I GTCTC 1 cut(s) 530
AlwI GGATC 3 cut(s) 485, 710, 723
AlwNI CAGNNNCTG 1 cut(s) 515
Ama87I CYCGRG 1 cut(s) 411
Aor13HI TCCGGA 1 cut(s) 325
AoxI GGCC 3 cut(s) 3, 40, 734
ApeKI GCWGC 2 cut(s) 407, 509
ApoI RAATTY 2 cut(s) 554, 573
AspLEI GCGC 3 cut(s) 210, 212, 594
AspS9I GGNCC 4 cut(s) 41, 100, 180, 734
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 3 cut(s) 209, 227, 442
AsuNHI GCTAGC 1 cut(s) 527
AvaI CYCGRG 1 cut(s) 411
AvaII GGWCC 2 cut(s) 100, 180
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 715
BanII GRGCYC 1 cut(s) 119
Bbv12I GWGCWC 2 cut(s) 119, 427
BbvCI CCTCAGC 1 cut(s) 387
BbvI GCAGC 2 cut(s) 419, 521
BccI CCATC 2 cut(s) 359, 728
BceAI ACGGC 1 cut(s) 685
BclI TGATCA 1 cut(s) 727
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 530
BfaI CTAG 1 cut(s) 528
BfmI CTRYAG 1 cut(s) 507
BisI GCNGC 8 cut(s) 11, 20, 23, 47, 65, 287, 408, 510
BlpI GCTNAGC 1 cut(s) 113
BlsI GCNGC 8 cut(s) 12, 21, 24, 48, 66, 288, 409, 511
Bme1390I CCNGG 1 cut(s) 69
Bme18I GGWCC 2 cut(s) 100, 180
BmeT110I CYCGRG 1 cut(s) 411
BmgBI CACGTC 1 cut(s) 422
BmgT120I GGNCC 4 cut(s) 41, 100, 180, 734
BmiI GGNNCC 2 cut(s) 285, 717
BmrFI CCNGG 1 cut(s) 69
BmsI GCATC 2 cut(s) 6, 467
BmtI GCTAGC 1 cut(s) 531
BplI GAGNNNNNCTC 2 cut(s) 656, 688
BpmI CTGGAG 1 cut(s) 675
Bpu10I CCTNAGC 1 cut(s) 387
Bpu1102I GCTNAGC 1 cut(s) 113
BpuMI CCSGG 1 cut(s) 69
BsaBI GATNNNNATC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 68, 522
BsaWI WCCGGW 3 cut(s) 83, 325, 709
Bse1I ACTGG 2 cut(s) 182, 658
Bse3DI GCAATG 1 cut(s) 403
Bse8I GATNNNNATC 1 cut(s) 135
BseAI TCCGGA 1 cut(s) 325
BseDI CCNNGG 2 cut(s) 68, 522
BseGI GGATG 3 cut(s) 300, 370, 753
BseJI GATNNNNATC 1 cut(s) 135
BseMI GCAATG 1 cut(s) 403
BseMII CTCAG 4 cut(s) 104, 401, 687, 695
BseNI ACTGG 2 cut(s) 182, 658
BsePI GCGCGC 1 cut(s) 208
BseXI GCAGC 2 cut(s) 419, 521
Bsh1236I CGCG 1 cut(s) 210
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 3 cut(s) 5, 42, 736
BsiEI CGRYCG 1 cut(s) 133
BsiHKAI GWGCWC 2 cut(s) 119, 427
BsiHKCI CYCGRG 1 cut(s) 411
BsiSI CCGG 5 cut(s) 68, 84, 326, 690, 710
BslFI GGGAC 3 cut(s) 85, 268, 332
BsmAI GTCTC 1 cut(s) 530
BsmFI GGGAC 3 cut(s) 85, 268, 332
BsnI GGCC 3 cut(s) 5, 42, 736
BsoBI CYCGRG 1 cut(s) 411
Bsp1286I GDGCHC 2 cut(s) 119, 427
Bsp13I TCCGGA 1 cut(s) 325
Bsp143I GATC 4 cut(s) 130, 490, 715, 727
Bsp1720I GCTNAGC 1 cut(s) 113
BspACI CCGC 9 cut(s) 11, 20, 23, 44, 47, 65, 287, 304, 617
BspANI GGCC 3 cut(s) 5, 42, 736
BspCNI CTCAG 4 cut(s) 105, 400, 686, 694
BspEI TCCGGA 1 cut(s) 325
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 2 cut(s) 285, 717
BspMAI CTGCAG 1 cut(s) 511
BspOI GCTAGC 1 cut(s) 531
BspPI GGATC 3 cut(s) 485, 710, 723
BsrDI GCAATG 1 cut(s) 403
BsrI ACTGG 2 cut(s) 182, 658
BssECI CCNNGG 2 cut(s) 68, 522
BssHII GCGCGC 1 cut(s) 208
BssMI GATC 4 cut(s) 130, 490, 715, 727
BssT1I CCWWGG 1 cut(s) 522
Bst4CI ACNGT 1 cut(s) 323
BstC8I GCNNGC 7 cut(s) 44, 210, 308, 529, 596, 687, 738
BstDEI CTNAG 4 cut(s) 113, 387, 673, 681
BstF5I GGATG 3 cut(s) 300, 370, 753
BstFNI CGCG 1 cut(s) 210
BstHHI GCGC 3 cut(s) 210, 212, 594
BstKTI GATC 4 cut(s) 133, 493, 718, 730
BstMAI GTCTC 1 cut(s) 530
BstMBI GATC 4 cut(s) 130, 490, 715, 727
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 3 cut(s) 16, 19, 205
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 1 cut(s) 67
BstSFI CTRYAG 1 cut(s) 507
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 2 cut(s) 419, 521
BstX2I RGATCY 1 cut(s) 715
BstYI RGATCY 1 cut(s) 715
BsuRI GGCC 3 cut(s) 5, 42, 736
BtrI CACGTC 1 cut(s) 422
BtsCI GGATG 3 cut(s) 300, 370, 753
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 2 cut(s) 453, 651
Cac8I GCNNGC 7 cut(s) 44, 210, 308, 529, 596, 687, 738
CaiI CAGNNNCTG 1 cut(s) 515
CfoI GCGC 3 cut(s) 210, 212, 594
Cfr13I GGNCC 4 cut(s) 41, 100, 180, 734
Csp6I GTAC 1 cut(s) 647
CviAII CATG 4 cut(s) 31, 79, 265, 343
CviQI GTAC 1 cut(s) 647
DdeI CTNAG 4 cut(s) 113, 387, 673, 681
DpnI GATC 4 cut(s) 132, 492, 717, 729
DpnII GATC 4 cut(s) 130, 490, 715, 727
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 117
Eco130I CCWWGG 1 cut(s) 522
Eco24I GRGCYC 1 cut(s) 119
Eco32I GATATC 1 cut(s) 229
Eco47I GGWCC 2 cut(s) 100, 180
Eco53kI GAGCTC 1 cut(s) 117
Eco88I CYCGRG 1 cut(s) 411
EcoICRI GAGCTC 1 cut(s) 117
EcoRV GATATC 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 522
EcoT38I GRGCYC 1 cut(s) 119
ErhI CCWWGG 1 cut(s) 522
FaeI CATG 4 cut(s) 34, 82, 268, 346
FaqI GGGAC 3 cut(s) 85, 268, 332
FatI CATG 4 cut(s) 30, 78, 264, 342
FauI CCCGC 1 cut(s) 51
FbaI TGATCA 1 cut(s) 727
Fnu4HI GCNGC 8 cut(s) 11, 20, 23, 47, 65, 287, 408, 510
FokI GGATG 2 cut(s) 287, 377
FriOI GRGCYC 1 cut(s) 119
Fsp4HI GCNGC 8 cut(s) 11, 20, 23, 47, 65, 287, 408, 510
FspBI CTAG 1 cut(s) 528
FspI TGCGCA 1 cut(s) 593
GlaI GCGC 3 cut(s) 209, 211, 593
GluI GCNGC 8 cut(s) 11, 20, 23, 47, 65, 287, 408, 510
GsuI CTGGAG 1 cut(s) 675
HaeIII GGCC 3 cut(s) 5, 42, 736
HapII CCGG 5 cut(s) 68, 84, 326, 690, 710
HhaI GCGC 3 cut(s) 210, 212, 594
Hin1II CATG 4 cut(s) 34, 82, 268, 346
Hin6I GCGC 3 cut(s) 208, 210, 592
HinP1I GCGC 3 cut(s) 208, 210, 592
HinfI GANTC 1 cut(s) 518
HpaII CCGG 5 cut(s) 68, 84, 326, 690, 710
HphI GGTGA 3 cut(s) 209, 227, 442
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 2 cut(s) 517, 676
Hpy188III TCNNGA 4 cut(s) 128, 224, 326, 494
Hpy8I GTNNAC 1 cut(s) 568
HpyAV CCTTC 2 cut(s) 178, 626
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4IV ACGT 2 cut(s) 421, 546
HpyCH4V TGCA 5 cut(s) 158, 396, 407, 458, 509
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 19, 205
HpyF3I CTNAG 4 cut(s) 113, 387, 673, 681
HpySE526I ACGT 2 cut(s) 421, 546
Hsp92II CATG 4 cut(s) 34, 82, 268, 346
HspAI GCGC 3 cut(s) 208, 210, 592
Kpn2I TCCGGA 1 cut(s) 325
Ksp22I TGATCA 1 cut(s) 727
Kzo9I GATC 4 cut(s) 130, 490, 715, 727
LmnI GCTCC 2 cut(s) 204, 283
Lsp1109I GCAGC 2 cut(s) 419, 521
LweI GCATC 2 cut(s) 6, 467
MaeI CTAG 1 cut(s) 528
MaeII ACGT 2 cut(s) 421, 546
MaeIII GTNAC 3 cut(s) 185, 277, 334
MalI GATC 4 cut(s) 132, 492, 717, 729
MboI GATC 4 cut(s) 130, 490, 715, 727
MboII GAAGA 3 cut(s) 453, 487, 569
MfeI CAATTG 1 cut(s) 540
MflI RGATCY 1 cut(s) 715
MhlI GDGCHC 2 cut(s) 119, 427
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 213, 540, 554, 573
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 397
MnlI CCTC 8 cut(s) 28, 135, 231, 248, 309, 396, 523, 717
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 325
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 558
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 5 cut(s) 68, 84, 326, 690, 710
MspR9I CCNGG 1 cut(s) 69
MunI CAATTG 1 cut(s) 540
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 3 cut(s) 16, 19, 205
NciI CCSGG 1 cut(s) 69
NdeII GATC 4 cut(s) 130, 490, 715, 727
NheI GCTAGC 1 cut(s) 527
NlaIII CATG 4 cut(s) 34, 82, 268, 346
NlaIV GGNNCC 2 cut(s) 285, 717
NmeAIII GCCGAG 1 cut(s) 688
NsbI TGCGCA 1 cut(s) 593
NspI RCATGY 1 cut(s) 268
PaeR7I CTCGAG 1 cut(s) 411
PauI GCGCGC 1 cut(s) 208
PcsI WCGNNNNNNNCGW 1 cut(s) 418
PfeI GAWTC 1 cut(s) 518
PkrI GCNGC 8 cut(s) 12, 21, 24, 48, 66, 288, 409, 511
Ple19I CGATCG 1 cut(s) 133
Psp124BI GAGCTC 1 cut(s) 119
PspN4I GGNNCC 2 cut(s) 285, 717
PspPI GGNCC 4 cut(s) 41, 100, 180, 734
PspXI VCTCGAGB 1 cut(s) 411
PstI CTGCAG 1 cut(s) 511
PstNI CAGNNNCTG 1 cut(s) 515
PsuI RGATCY 1 cut(s) 715
PteI GCGCGC 1 cut(s) 208
PvuI CGATCG 1 cut(s) 133
RsaI GTAC 1 cut(s) 648
RsaNI GTAC 1 cut(s) 647
SacI GAGCTC 1 cut(s) 119
SaqAI TTAA 1 cut(s) 558
SatI GCNGC 8 cut(s) 11, 20, 23, 47, 65, 287, 408, 510
Sau3AI GATC 4 cut(s) 130, 490, 715, 727
Sau96I GGNCC 4 cut(s) 41, 100, 180, 734
ScrFI CCNGG 1 cut(s) 69
SduI GDGCHC 2 cut(s) 119, 427
SfaNI GCATC 2 cut(s) 6, 467
SfcI CTRYAG 1 cut(s) 507
Sfr274I CTCGAG 1 cut(s) 411
SinI GGWCC 2 cut(s) 100, 180
SlaI CTCGAG 1 cut(s) 411
SmlI CTYRAG 1 cut(s) 411
SmoI CTYRAG 1 cut(s) 411
Sse9I AATT 4 cut(s) 213, 540, 554, 573
SsiI CCGC 9 cut(s) 11, 20, 23, 44, 47, 65, 287, 304, 617
SspI AATATT 1 cut(s) 755
SspMI CTAG 1 cut(s) 528
SstI GAGCTC 1 cut(s) 119
StyD4I CCNGG 1 cut(s) 67
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 1 cut(s) 323
TaiI ACGT 2 cut(s) 424, 549
TaqI TCGA 4 cut(s) 129, 371, 412, 562
TasI AATT 4 cut(s) 213, 540, 554, 573
TauI GCSGC 6 cut(s) 13, 22, 25, 49, 67, 289
TfiI GAWTC 1 cut(s) 518
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TscAI CASTG 2 cut(s) 460, 658
TseI GCWGC 2 cut(s) 407, 509
TspDTI ATGAA 2 cut(s) 207, 516
TspGWI ACGGA 4 cut(s) 89, 191, 287, 296
TspRI CASTG 2 cut(s) 460, 658
VpaK11BI GGWCC 2 cut(s) 100, 180
XapI RAATTY 2 cut(s) 554, 573
XceI RCATGY 1 cut(s) 268
XhoI CTCGAG 1 cut(s) 411
XspI CTAG 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.