RLG00000020470

60S ribosomal Protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001527
Physical Location & Seq
Forward (+)
65390736 .. 65392207
1472 bp
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UTR
Exon/CDS
Intron
RLM00000020470

Sequence Viewer

Length: 393 bp
ATGGGTGAAGCAGTCAAGGTAGTGGTTCCAGAGTCTGTCCTAAAGAAGAGGAAGAGAGAGGAGGAATGGGCTTTGGCAAGGAAGCAGGGGCTAGAAGCTGCCAAGAAGAAGAATGCTGAGAATAGGAAGCTTATTTACAACAAATCCAAACAGTACACCAAGGAGTACGTAGAGAAGGGTTTGGGCAAGCATGGCATTATCTGCACGGAAGATCTTATCCACGAGATCTTGACAGTTGGACCTCATTTCAAGGAGGCCAACAACTTCCTATGGCCATTCAAGCTCAAGGCACCATTGGGTGGTCTTAAGAAGAAGAGGAACCATTATGTTGAAGGTGGAGATGCTGGAAACCGTGAGAACTACATCAATGAGCTTATTAGGAGAATGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L30 Ribosomal_L30_N

Protein Analysis

131

Amino Acids

15.02

Weight (kDa)

9.76

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L30_N PF08079 9 - 59 3.3e-12 Ribosomal L30 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 289
AccB7I CCANNNNNTGG 1 cut(s) 299
AcoI YGGCCR 1 cut(s) 272
AfaI GTAC 2 cut(s) 155, 167
AfiI CCNNNNNNNGG 1 cut(s) 299
AflII CTTAAG 1 cut(s) 305
AgsI TTSAA 3 cut(s) 250, 280, 332
AluBI AGCT 4 cut(s) 98, 130, 283, 373
AluI AGCT 4 cut(s) 98, 130, 283, 373
AlwNI CAGNNNCTG 1 cut(s) 35
AoxI GGCC 2 cut(s) 255, 272
ApeKI GCWGC 1 cut(s) 98
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 1 cut(s) 17
AvaII GGWCC 1 cut(s) 239
BalI TGGCCA 1 cut(s) 274
BanI GGYRCC 1 cut(s) 289
BarI GAAGNNNNNNTAC 2 cut(s) 119, 151
BauI CACGAG 1 cut(s) 221
BbvI GCAGC 1 cut(s) 85
BfaI CTAG 2 cut(s) 92, 391
BfrI CTTAAG 1 cut(s) 305
BglII AGATCT 2 cut(s) 211, 225
BisI GCNGC 1 cut(s) 99
BlsI GCNGC 1 cut(s) 100
Bme18I GGWCC 1 cut(s) 239
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 3 cut(s) 27, 291, 320
BmsI GCATC 1 cut(s) 331
BpuEI CTTGAG 1 cut(s) 269
BsaAI YACGTR 1 cut(s) 169
BsaJI CCNNGG 1 cut(s) 159
Bsc4I CCNNNNNNNGG 1 cut(s) 299
BseDI CCNNGG 1 cut(s) 159
BseLI CCNNNNNNNGG 1 cut(s) 299
BseMII CTCAG 1 cut(s) 108
BseRI GAGGAG 1 cut(s) 74
BseXI GCAGC 1 cut(s) 85
BsgI GTGCAG 1 cut(s) 187
BshFI GGCC 2 cut(s) 257, 274
BshNI GGYRCC 1 cut(s) 289
BslI CCNNNNNNNGG 1 cut(s) 299
BsmI GAATGC 1 cut(s) 118
BsnI GGCC 2 cut(s) 257, 274
Bsp143I GATC 2 cut(s) 211, 225
BspANI GGCC 2 cut(s) 257, 274
BspCNI CTCAG 1 cut(s) 109
BspLI GGNNCC 3 cut(s) 27, 291, 320
BspT107I GGYRCC 1 cut(s) 289
BspTI CTTAAG 1 cut(s) 305
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 2 cut(s) 211, 225
BssSI CACGAG 1 cut(s) 221
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 221
Bst4CI ACNGT 3 cut(s) 153, 235, 353
Bst6I CTCTTC 3 cut(s) 41, 47, 308
BstAFI CTTAAG 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 201
BstBAI YACGTR 1 cut(s) 169
BstC8I GCNNGC 1 cut(s) 188
BstDEI CTNAG 1 cut(s) 117
BstKTI GATC 2 cut(s) 214, 228
BstMBI GATC 2 cut(s) 211, 225
BstMWI GCNNNNNNNGC 3 cut(s) 192, 201, 280
BstSNI TACGTA 1 cut(s) 169
BstV1I GCAGC 1 cut(s) 85
BstX2I RGATCY 2 cut(s) 211, 225
BstYI RGATCY 2 cut(s) 211, 225
BsuRI GGCC 2 cut(s) 257, 274
Cac8I GCNNGC 1 cut(s) 188
CaiI CAGNNNCTG 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 239
Csp6I GTAC 2 cut(s) 154, 166
CviAII CATG 1 cut(s) 191
CviJI RGCY 8 cut(s) 71, 91, 98, 130, 257, 274, 283, 373
CviKI_1 RGCY 8 cut(s) 71, 91, 98, 130, 257, 274, 283, 373
CviQI GTAC 2 cut(s) 154, 166
DdeI CTNAG 1 cut(s) 117
DpnI GATC 2 cut(s) 213, 227
DpnII GATC 2 cut(s) 211, 225
EaeI YGGCCR 1 cut(s) 272
Eam1104I CTCTTC 3 cut(s) 41, 47, 308
EarI CTCTTC 3 cut(s) 41, 47, 308
Eco105I TACGTA 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 1 cut(s) 194
FaiI YATR 3 cut(s) 192, 271, 327
FatI CATG 1 cut(s) 190
Fnu4HI GCNGC 1 cut(s) 99
Fsp4HI GCNGC 1 cut(s) 99
FspBI CTAG 2 cut(s) 92, 391
GluI GCNGC 1 cut(s) 99
HaeIII GGCC 2 cut(s) 257, 274
Hin1II CATG 1 cut(s) 194
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 1 cut(s) 32
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 1 cut(s) 156
Hpy188III TCNNGA 2 cut(s) 29, 229
Hpy8I GTNNAC 1 cut(s) 156
HpyAV CCTTC 2 cut(s) 169, 326
HpyCH4III ACNGT 3 cut(s) 153, 235, 353
HpyCH4IV ACGT 1 cut(s) 168
HpyCH4V TGCA 1 cut(s) 204
HpyF10VI GCNNNNNNNGC 3 cut(s) 192, 201, 280
HpyF3I CTNAG 1 cut(s) 117
HpySE526I ACGT 1 cut(s) 168
Hsp92II CATG 1 cut(s) 194
Kzo9I GATC 2 cut(s) 211, 225
LpnPI CCDG 3 cut(s) 42, 71, 330
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 1 cut(s) 331
MaeI CTAG 2 cut(s) 92, 391
MaeII ACGT 1 cut(s) 168
MalI GATC 2 cut(s) 213, 227
MboI GATC 2 cut(s) 211, 225
MboII GAAGA 7 cut(s) 58, 64, 118, 121, 221, 322, 325
MflI RGATCY 2 cut(s) 211, 225
MlsI TGGCCA 1 cut(s) 274
MluNI TGGCCA 1 cut(s) 274
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 6 cut(s) 42, 52, 55, 247, 252, 309
Mox20I TGGCCA 1 cut(s) 274
MscI TGGCCA 1 cut(s) 274
MseI TTAA 1 cut(s) 306
Msp20I TGGCCA 1 cut(s) 274
MspCI CTTAAG 1 cut(s) 305
Mva1269I GAATGC 1 cut(s) 118
MwoI GCNNNNNNNGC 3 cut(s) 192, 201, 280
NdeII GATC 2 cut(s) 211, 225
NlaIII CATG 1 cut(s) 194
NlaIV GGNNCC 3 cut(s) 27, 291, 320
PctI GAATGC 1 cut(s) 118
PflMI CCANNNNNTGG 1 cut(s) 299
PkrI GCNGC 1 cut(s) 100
PleI GAGTC 1 cut(s) 40
PpsI GAGTC 1 cut(s) 40
Ppu21I YACGTR 1 cut(s) 169
PspN4I GGNNCC 3 cut(s) 27, 291, 320
PspPI GGNCC 1 cut(s) 239
PstNI CAGNNNCTG 1 cut(s) 35
PsuI RGATCY 2 cut(s) 211, 225
RsaI GTAC 2 cut(s) 155, 167
RsaNI GTAC 2 cut(s) 154, 166
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 1 cut(s) 99
Sau3AI GATC 2 cut(s) 211, 225
Sau96I GGNCC 1 cut(s) 239
SchI GAGTC 1 cut(s) 41
SetI ASST 8 cut(s) 21, 100, 132, 171, 244, 285, 337, 375
SfaNI GCATC 1 cut(s) 331
SinI GGWCC 1 cut(s) 239
SmlI CTYRAG 2 cut(s) 284, 305
SmoI CTYRAG 2 cut(s) 284, 305
SnaBI TACGTA 1 cut(s) 169
SspMI CTAG 2 cut(s) 92, 391
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 153, 235, 353
TaiI ACGT 1 cut(s) 171
TatI WGTACW 1 cut(s) 153
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TseI GCWGC 1 cut(s) 98
TspGWI ACGGA 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 299
Vha464I CTTAAG 1 cut(s) 305
VpaK11BI GGWCC 1 cut(s) 239
XspI CTAG 2 cut(s) 92, 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.