Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000030.1

Orthogroup ID

OG0002606

Physical Location & Seq

Genomic Coordinates Forward (+)

118242 .. 126619

Gene Structure 8378 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3264 bp

atgccccgtcacagagacgaagacgacgacgagatggagccggacgaggacgagtacgacccggaccaaggtctagacgacgtcgacgtcgacgacgaagaggaggaggaggaggaggaggaggaccgaggagggcggaggtccagtcagaagcggaggagatcgagcttcattgatgacgtggccgaggaggacgacgaggaggaagaggaggactacgaggacgaggattacggcgcctaccgtggcggtggcggtgcgaggaggaggaggaggaggaccgaggagggcggaggtccagaggacgacgaggaggaagaggaggactacgaggacgaggattacggcgcctaccgtggcggtggcggtggcggtgggagtcgtaagaagagcaaaaagaagccctccggtgcggattttcttgatctcgaagcggtggtggattcggatgaggaggaagaggaggatgagggcgaggacgactttattgattccggagctgatatacctgaggagggcgatggtagaaggatgcatcatcgcccattgatggatgaccaagagcaagaagatgtcgaggctctggaaagaagaattcaggcaagatatgcaaagcaacatcatacagaagatactgaggagaccactgatgtggatcagcaagctcttttaccttctgttttggacccaaagctgtggatggtgaaatgtgcgattggtaaagaacgagaggtagctgcttgcttaatgcaaaagtatattgataaacctgaattgaacatcaggtctgttattgctcttgaccacctgaagaactatatatacgttgaagcggaaaaagaagcccatgtgagggaggcttgcaaaggattgcgcaatatatttacccagaaaatagcgcttgttcctattagagaaatgactgatgttctttccgttgaaagcaaggcaattgatatttctagggatacttgggtcagaatgaagattgggatatataaaggggaccttgccaaggttgttgatgtggataatgtacgacagagagttacagtaaaattaattccaagaattgatttacaagctcttgcaaataaactggagggcagagaagttgtgaaaaagaaagcgtttgtacctccgccacgttttatgaatattgatgaagctagagaactgcatattcgtgtggagcgtaaacgagatccaatgacgggtgactactttgaaaacattgagaaaatgatgtttaaagatggtttcttgtataaaatagtatcaatgaaatcaattagtactcaaaacatacatcccacttttgatgaactggaaaagtttcggaaacctggggagaatggggaaggagatgttgccagtctgtcaactttattttcaaacagaaagaaaggacactttgtgaagggtgatgcagtcattgtcatcaagggagacctaaaaaacttgaaagggcgggttgagaaagttgaggaagggactgtgcatatcagacccgacatgaaagacctcccaaaaactcttgccataaatgaaaaagaactttgcaagtactttgaacctgggaatcatgtgaaggttgtatctggtactcaagaaggtgcaactggtatggttgtcaaggtcgatcagcatgtgctgatcattttatctgatacaactaaggaacatctccgtgtgtttgctgatgatgttgtggagagttctgaggtgacatctggtattaccaaaattggggactatgagcttcatgatcttgtgttactggataataatagctttggtgtaattatacgtgttgaaactgaagcatgtcaggtccttaagggagttcctgagagacctgaggttgcccttgtcaaattaagagagatcaaatgcaagattgagaaaaagcttagtgtgcaagattgttttaagaacacaatatctgtgaaagatgttgtgagggtcgttgaaggtccttccaagggtaaacaaggtcccgtggaacacatatacagaggagtcttattcatttatgatcgccatcacatagagcatgcaggctttatctgtgttaagtcacattcttgtcgtgttgtaggaggatcacgtgccaatggtgatagaaatggtgactcctactctagatttgatcatcttcgagctcctcctcttattccccagtcccccaggaggtttcctagaggaggccctcctaataactttgggggaagaaatagaggtggaagagggcatgatggattggttgggactacagtaaagattcgccagggtgcctataagggttaccgtgggcgcgttgttgaggttaagggaactagtgtacgggtggaacttgagtcccaaatgaaagttgtgacagttgaccggaattgtatttctgataacgtggctattacaacaccgtatcgtgatacatctagctatggtatgggaagtcagaccccaattcatccttctcgaactcctctccatccatatatgacgccaatgagggatgctggagcaacgcctattcatgatggcatgaggactccaatgcgtgaccgagcatggaatccatatgcacctatgagtccagcaagggatagctgggaggatggaaaccctggttcttggggttctagcccacagtatcagccaggaagtcctccctcacggacgtacgaagctccaactcctggttcaggttgggccaacactcctggtgggaattatagtgaagctggaacaccaagggacagtactggttatgcaaatgctcctagcccatacttgccgtcaactcccggtgggcagcctatgacaccaaactcagcatcctatcttcccggaactcctggagggcagccaatgacaccagggactggtggtctggatatgatgtctcctgttataggaggggacaatgaaggaccatggttcatgccagatatactggtcaatgtacgcaattctggagaggaaactactggaattgtcagagaagtgctcctggatggctcatgtagggtagctcttggctcgggtggcaatggtgatactgtaactgtacttccaaatgaaatcgaggtagtcgtaccaaggaagaatgataagatcaaaatcatgggtggctcattacgcggtgccactggcaagctgattggtgtcgatggcacggatggaattgtcaaggttgatgacactcttgatgttaaaatattagacttggctattttgtcaaaactaggtcaatcataa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016071 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032044 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KOW Spt5-NGN Spt5_N

Protein Analysis

1087

Amino Acids

120.72

Weight (kDa)

5.08

Isoelectric Point (pI)

47.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spt5_N	PF11942	140 - 225	3.8e-12	Spt5 transcription elongation factor, acidic N-terminal
Spt5-NGN	PF03439	231 - 311	8.6e-27	Early transcription elongation factor of RNA pol II, NGN section
KOW1_SPT5	PF23042	317 - 446	5.3e-76	Transcription elongation factor SPT5, KOW1 domain
KOW2_Spt5	PF23284	474 - 523	4.3e-22	Transcription elongation factor SPT5, second KOW domain
KOWx_SPT5	PF23037	582 - 633	1.4e-17	Transcription elongation factor SPT5, KOWx domain
KOW4_SPT5	PF23291	641 - 694	7.3e-24	Transcription elongation factor SPT5, fourth KOW domain
KOW5_SPT5	PF23290	755 - 798	7.5e-18	Transcription elongation factor SPT5, fifth KOW domain
KOW6_SPT51-2	PF23038	982 - 1031	6.4e-26	Transcription elongation factor SPT5 homolog 1/2, KOW6 domain
KOW7_SPT5	PF23287	1033 - 1084	4e-21	Transcription elongation factor SPT5, seventh KOW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002606)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G08350
fragaria_vesca	FvH4_2g14770 FvH4_2g14770 FvH4_2g14770 FvH4_7g15891 FvH4_7g15900
malus_domestica	MD01G1075900.v1.1 MD05G1013100.v1.1 MD10G1013600.v1.1
prunus_persica	Prupe.2G047200_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1 Prupe.8G018000_v2.0.a1
pyrus_communis	pycom01g10480 pycom05g00760 pycom10g00830
rosa_chinensis	RchiOBHm_Chr1g0358771 RchiOBHm_Chr6g0277331
rosa_laevigata	RLG00000013309 RLG00000015056 RLG00000027924 RLG00000027930 RLG00000027931
rosa_multiflora	Rmu_co8026512.1_g000001 Rmu_sc0000030.1_g000027 Rmu_sc0017529.1_g000003 Rmu_sc0031713.1_g000002
rosa_roxburghii	Rroxscaffold_4G00297640 Rroxscaffold_7G00191440
rosa_rugosa	Rorug01G0264800 Rorug01G0265100 Rorug06G0107200
rosa_samantha	Rh1AG279200 Rh1BG245800 Rh1CG263000 Rh1DG274100 Rh6BG222000 Rh6DG215000
rosa_wichuraiana	Rw1G024810 Rw6G019000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	86
AatII	GACGTC	2 cut(s)	84, 90
Acc16I	TGCGCA	1 cut(s)	875
AccB1I	GGYRCC	4 cut(s)	236, 347, 2294, 3149
AccB7I	CCANNNNNTGG	2 cut(s)	2702, 2888
AccI	GTMKAC	2 cut(s)	84, 90
AccII	CGCG	2 cut(s)	2319, 3147
AccIII	TCCGGA	1 cut(s)	494
AclWI	GGATC	3 cut(s)	663, 1199, 2113
AcoI	YGGCCR	1 cut(s)	183
AcsI	RAATTY	1 cut(s)	594
AcuI	CTGAAG	2 cut(s)	830, 1842
AcvI	CACGTG	1 cut(s)	2111
AcyI	GRCGYC	5 cut(s)	81, 87, 237, 348, 2507
AdeI	CACNNNGTG	1 cut(s)	1417
AfeI	AGCGCT	1 cut(s)	900
AflII	CTTAAG	1 cut(s)	1838
AflIII	ACRYGT	1 cut(s)	1810
AgsI	TTSAA	9 cut(s)	778, 830, 941, 1229, 1395, 1465, 1574, 1817, 1973
AhdI	GACNNNNNGTC	1 cut(s)	2892
AhlI	ACTAGT	1 cut(s)	2339
AjiI	CACGTC	1 cut(s)	181
AjuI	GAANNNNNNNTTGG	4 cut(s)	2461, 2493, 2689, 2721
AleI	CACNNNNGTG	1 cut(s)	650
Alw21I	GWGCWC	2 cut(s)	2167, 3015
Alw26I	GTCTC	5 cut(s)	9, 635, 1443, 1849, 2913
AlwI	GGATC	3 cut(s)	663, 1199, 2113
AlwNI	CAGNNNCTG	1 cut(s)	2888
Ama87I	CYCGRG	1 cut(s)	3046
Aor13HI	TCCGGA	1 cut(s)	494
Aor51HI	AGCGCT	1 cut(s)	900
AoxI	GGCC	3 cut(s)	183, 2209, 2715
ApeKI	GCWGC	3 cut(s)	737, 2818, 2869
ApoI	RAATTY	1 cut(s)	594
AseI	ATTAAT	1 cut(s)	1061
Asp700I	GAANNNNTTC	1 cut(s)	1335
AspLEI	GCGC	5 cut(s)	239, 350, 876, 901, 2319
AsuC2I	CCSGG	3 cut(s)	62, 2811, 2853
AsuHPI	GGTGA	7 cut(s)	715, 1229, 1436, 1738, 2132, 2144, 3071
AvaI	CYCGRG	1 cut(s)	3046
AxyI	CCTNAGG	2 cut(s)	510, 1860
BaeI	ACNNNNGTAYC	2 cut(s)	960, 993
BanI	GGYRCC	4 cut(s)	236, 347, 2294, 3149
BanII	GRGCYC	1 cut(s)	2167
BarI	GAAGNNNNNNTAC	2 cut(s)	3060, 3092
BbrPI	CACGTG	1 cut(s)	2111
BbsI	GAAGAC	1 cut(s)	27
Bbv12I	GWGCWC	2 cut(s)	2167, 3015
BbvI	GCAGC	3 cut(s)	724, 2830, 2881
BceAI	ACGGC	3 cut(s)	250, 361, 2785
BciVI	GTATCC	1 cut(s)	961
BclI	TGATCA	2 cut(s)	1656, 2152
BcnI	CCSGG	3 cut(s)	62, 2811, 2853
BcoDI	GTCTC	5 cut(s)	9, 635, 1443, 1849, 2913
BcuI	ACTAGT	1 cut(s)	2339
BfmI	CTRYAG	1 cut(s)	2274
BfoI	RGCGCY	3 cut(s)	240, 351, 902
BfrI	CTTAAG	1 cut(s)	1838
BfuI	GTATCC	1 cut(s)	961
BisI	GCNGC	3 cut(s)	738, 2819, 2870
BlsI	GCNGC	3 cut(s)	739, 2820, 2871
BmcAI	AGTACT	3 cut(s)	1297, 1568, 2767
BmeRI	GACNNNNNGTC	1 cut(s)	2892
BmeT110I	CYCGRG	1 cut(s)	3046
BmgBI	CACGTC	1 cut(s)	181
BmiI	GGNNCC	8 cut(s)	39, 238, 349, 687, 1007, 1999, 2296, 3151
BmrI	ACTGGG	1 cut(s)	2176
BmsI	GCATC	5 cut(s)	522, 544, 1417, 2509, 2849
BmuI	ACTGGG	1 cut(s)	2176
BoxI	GACNNNNGTC	1 cut(s)	69
BpiI	GAAGAC	1 cut(s)	27
BplI	GAGNNNNNCTC	2 cut(s)	2997, 3029
BpmI	CTGGAG	4 cut(s)	1121, 2544, 2883, 3000
BpuEI	CTTGAG	2 cut(s)	1593, 2378
BpuMI	CCSGG	3 cut(s)	62, 2811, 2853
BsaAI	YACGTR	2 cut(s)	1811, 2111
BsaBI	GATNNNNATC	3 cut(s)	654, 2043, 3125
BsaHI	GRCGYC	5 cut(s)	81, 87, 237, 348, 2507
BsaI	GGTCTC	3 cut(s)	635, 1443, 1849
BsaWI	WCCGGW	3 cut(s)	407, 494, 2388
BsaXI	ACNNNNNCTCC	2 cut(s)	130, 160
Bse21I	CCTNAGG	2 cut(s)	510, 1860
Bse3DI	GCAATG	1 cut(s)	3061
Bse8I	GATNNNNATC	3 cut(s)	654, 2043, 3125
BseAI	TCCGGA	1 cut(s)	494
BseJI	GATNNNNATC	3 cut(s)	654, 2043, 3125
BseMI	GCAATG	1 cut(s)	3061
BseMII	CTCAG	6 cut(s)	501, 627, 1713, 1842, 1851, 2850
BseXI	GCAGC	3 cut(s)	724, 2830, 2881
BseYI	CCCAGC	1 cut(s)	2613
Bsh1236I	CGCG	2 cut(s)	2319, 3147
BshFI	GGCC	3 cut(s)	185, 2211, 2717
BshNI	GGYRCC	4 cut(s)	236, 347, 2294, 3149
BsiHKAI	GWGCWC	2 cut(s)	2167, 3015
BsiHKCI	CYCGRG	1 cut(s)	3046
BsiSI	CCGG	7 cut(s)	41, 62, 408, 495, 2389, 2811, 2853
BsiWI	CGTACG	1 cut(s)	2685
BsmAI	GTCTC	5 cut(s)	9, 635, 1443, 1849, 2913
BsmBI	CGTCTC	1 cut(s)	9
BsnI	GGCC	3 cut(s)	185, 2211, 2717
Bso31I	GGTCTC	3 cut(s)	635, 1443, 1849
BsoBI	CYCGRG	1 cut(s)	3046
Bsp1286I	GDGCHC	2 cut(s)	2167, 3015
Bsp13I	TCCGGA	1 cut(s)	494
Bsp19I	CCATGG	1 cut(s)	2939
BspANI	GGCC	3 cut(s)	185, 2211, 2717
BspCNI	CTCAG	6 cut(s)	502, 628, 1714, 1843, 1852, 2849
BspEI	TCCGGA	1 cut(s)	494
BspFNI	CGCG	2 cut(s)	2319, 3147
BspHI	TCATGA	2 cut(s)	1765, 2539
BspLI	GGNNCC	8 cut(s)	39, 238, 349, 687, 1007, 1999, 2296, 3151
BspPI	GGATC	3 cut(s)	663, 1199, 2113
BspQI	GCTCTTC	1 cut(s)	383
BspT107I	GGYRCC	4 cut(s)	236, 347, 2294, 3149
BspTI	CTTAAG	1 cut(s)	1838
BspTNI	GGTCTC	3 cut(s)	635, 1443, 1849
BsrDI	GCAATG	1 cut(s)	3061
BssNI	GRCGYC	5 cut(s)	81, 87, 237, 348, 2507
BssT1I	CCWWGG	6 cut(s)	67, 1014, 1983, 2756, 2939, 3104
Bst6I	CTCTTC	6 cut(s)	93, 201, 312, 383, 453, 2242
BstACI	GRCGYC	5 cut(s)	81, 87, 237, 348, 2507
BstAFI	CTTAAG	1 cut(s)	1838
BstAPI	GCANNNNNTGC	1 cut(s)	608
BstBAI	YACGTR	2 cut(s)	1811, 2111
BstC8I	GCNNGC	6 cut(s)	663, 742, 862, 2058, 2062, 3161
BstDEI	CTNAG	8 cut(s)	510, 636, 1677, 1722, 1851, 1860, 1913, 2836
BstDSI	CCRYGG	5 cut(s)	244, 355, 2001, 2311, 2939
BstEII	GGTNACC	1 cut(s)	2306
BstENI	CCTNNNNNAGG	4 cut(s)	1013, 2205, 2706, 2916
BstFNI	CGCG	2 cut(s)	2319, 3147
BstH2I	RGCGCY	3 cut(s)	240, 351, 902
BstHHI	GCGC	5 cut(s)	239, 350, 876, 901, 2319
BstMAI	GTCTC	5 cut(s)	9, 635, 1443, 1849, 2913
BstMWI	GCNNNNNNNGC	4 cut(s)	608, 1918, 3051, 3144
BstNSI	RCATGY	3 cut(s)	1652, 1830, 2060
BstPAI	GACNNNNGTC	1 cut(s)	69
BstPI	GGTNACC	1 cut(s)	2306
BstSFI	CTRYAG	1 cut(s)	2274
BstUI	CGCG	2 cut(s)	2319, 3147
BstV1I	GCAGC	3 cut(s)	724, 2830, 2881
BstV2I	GAAGAC	1 cut(s)	27
BstX2I	RGATCY	1 cut(s)	1204
BstXI	CCANNNNNNTGG	2 cut(s)	652, 696
BstYI	RGATCY	1 cut(s)	1204
Bsu36I	CCTNAGG	2 cut(s)	510, 1860
BsuI	GTATCC	1 cut(s)	961
BsuRI	GGCC	3 cut(s)	185, 2211, 2717
BtgI	CCRYGG	5 cut(s)	244, 355, 2001, 2311, 2939
BtgZI	GCGATG	2 cut(s)	524, 534
BtrI	CACGTC	1 cut(s)	181
BtsIMutI	CAGTG	2 cut(s)	645, 3153
Cac8I	GCNNGC	6 cut(s)	663, 742, 862, 2058, 2062, 3161
CaiI	CAGNNNCTG	1 cut(s)	2888
CciI	TCATGA	2 cut(s)	1765, 2539
CfoI	GCGC	5 cut(s)	239, 350, 876, 901, 2319
CseI	GACGC	1 cut(s)	2515
DdeI	CTNAG	8 cut(s)	510, 636, 1677, 1722, 1851, 1860, 1913, 2836
DinI	GGCGCC	2 cut(s)	238, 349
DraI	TTTAAA	1 cut(s)	1252
DraIII	CACNNNGTG	1 cut(s)	1417
DrdI	GACNNNNNNGTC	1 cut(s)	86
DriI	GACNNNNNGTC	1 cut(s)	2892
DseDI	GACNNNNNNGTC	1 cut(s)	86
EaeI	YGGCCR	1 cut(s)	183
Eam1104I	CTCTTC	6 cut(s)	93, 201, 312, 383, 453, 2242
Eam1105I	GACNNNNNGTC	1 cut(s)	2892
EarI	CTCTTC	6 cut(s)	93, 201, 312, 383, 453, 2242
EciI	GGCGGA	3 cut(s)	151, 306, 1131
Ecl136II	GAGCTC	1 cut(s)	2165
Eco130I	CCWWGG	6 cut(s)	67, 1014, 1983, 2756, 2939, 3104
Eco24I	GRGCYC	1 cut(s)	2167
Eco31I	GGTCTC	3 cut(s)	635, 1443, 1849
Eco47III	AGCGCT	1 cut(s)	900
Eco53kI	GAGCTC	1 cut(s)	2165
Eco57I	CTGAAG	2 cut(s)	830, 1842
Eco72I	CACGTG	1 cut(s)	2111
Eco81I	CCTNAGG	2 cut(s)	510, 1860
Eco88I	CYCGRG	1 cut(s)	3046
Eco91I	GGTNACC	1 cut(s)	2306
EcoICRI	GAGCTC	1 cut(s)	2165
EcoNI	CCTNNNNNAGG	4 cut(s)	1013, 2205, 2706, 2916
EcoO109I	RGGNCCY	5 cut(s)	1006, 1834, 1976, 1997, 2210
EcoO65I	GGTNACC	1 cut(s)	2306
EcoRI	GAATTC	1 cut(s)	594
EcoT14I	CCWWGG	6 cut(s)	67, 1014, 1983, 2756, 2939, 3104
EcoT22I	ATGCAT	1 cut(s)	537
EcoT38I	GRGCYC	1 cut(s)	2167
EgeI	GGCGCC	2 cut(s)	238, 349
EheI	GGCGCC	2 cut(s)	238, 349
ErhI	CCWWGG	6 cut(s)	67, 1014, 1983, 2756, 2939, 3104
Esp3I	CGTCTC	1 cut(s)	9
FalI	AAGNNNNNCTT	2 cut(s)	993, 1025
FauI	CCCGC	1 cut(s)	1464
FauNDI	CATATG	1 cut(s)	2584
FbaI	TGATCA	2 cut(s)	1656, 2152
FblI	GTMKAC	2 cut(s)	84, 90
Fnu4HI	GCNGC	3 cut(s)	738, 2819, 2870
FriOI	GRGCYC	1 cut(s)	2167
Fsp4HI	GCNGC	3 cut(s)	738, 2819, 2870
FspI	TGCGCA	1 cut(s)	875
GlaI	GCGC	5 cut(s)	238, 349, 875, 900, 2318
GluI	GCNGC	3 cut(s)	738, 2819, 2870
GsaI	CCCAGC	1 cut(s)	2617
GsuI	CTGGAG	4 cut(s)	1121, 2544, 2883, 3000
HaeII	RGCGCY	3 cut(s)	240, 351, 902
HaeIII	GGCC	3 cut(s)	185, 2211, 2717
HapII	CCGG	7 cut(s)	41, 62, 408, 495, 2389, 2811, 2853
HgaI	GACGC	1 cut(s)	2515
HhaI	GCGC	5 cut(s)	239, 350, 876, 901, 2319
Hin1I	GRCGYC	5 cut(s)	81, 87, 237, 348, 2507
Hin6I	GCGC	5 cut(s)	237, 348, 874, 899, 2317
HinP1I	GCGC	5 cut(s)	237, 348, 874, 899, 2317
HincII	GTYRAC	5 cut(s)	85, 91, 1383, 2386, 2805
HindII	GTYRAC	5 cut(s)	85, 91, 1383, 2386, 2805
HindIII	AAGCTT	1 cut(s)	1910
HpaII	CCGG	7 cut(s)	41, 62, 408, 495, 2389, 2811, 2853
HphI	GGTGA	7 cut(s)	715, 1229, 1436, 1738, 2132, 2144, 3071
Hpy166II	GTNNAC	8 cut(s)	85, 91, 1199, 1383, 1991, 2345, 2386, 2805
Hpy8I	GTNNAC	8 cut(s)	85, 91, 1199, 1383, 1991, 2345, 2386, 2805
HpyCH4IV	ACGT	9 cut(s)	81, 87, 180, 825, 1147, 1810, 2110, 2409, 2684
HpyF10VI	GCNNNNNNNGC	4 cut(s)	608, 1918, 3051, 3144
HpyF3I	CTNAG	8 cut(s)	510, 636, 1677, 1722, 1851, 1860, 1913, 2836
HpySE526I	ACGT	9 cut(s)	81, 87, 180, 825, 1147, 1810, 2110, 2409, 2684
Hsp92I	GRCGYC	5 cut(s)	81, 87, 237, 348, 2507
HspAI	GCGC	5 cut(s)	237, 348, 874, 899, 2317
KasI	GGCGCC	2 cut(s)	236, 347
Kpn2I	TCCGGA	1 cut(s)	494
Ksp22I	TGATCA	2 cut(s)	1656, 2152
LguI	GCTCTTC	1 cut(s)	383
LmnI	GCTCC	8 cut(s)	37, 497, 1192, 2170, 2525, 2698, 2788, 3018
Lsp1109I	GCAGC	3 cut(s)	724, 2830, 2881
LweI	GCATC	5 cut(s)	522, 544, 1417, 2509, 2849
MaeII	ACGT	9 cut(s)	81, 87, 180, 825, 1147, 1810, 2110, 2409, 2684
MfeI	CAATTG	1 cut(s)	951
MflI	RGATCY	1 cut(s)	1204
MhlI	GDGCHC	2 cut(s)	2167, 3015
Mly113I	GGCGCC	2 cut(s)	237, 348
MlyI	GAGTC	6 cut(s)	388, 2031, 2129, 2369, 2548, 2605
MmeI	TCCRAC	1 cut(s)	2720
Mph1103I	ATGCAT	1 cut(s)	537
MroI	TCCGGA	1 cut(s)	494
MroXI	GAANNNNTTC	1 cut(s)	1335
MseI	TTAA	9 cut(s)	746, 1061, 1251, 1839, 1880, 1932, 2076, 2331, 3219
MslI	CAYNNNNRTG	3 cut(s)	650, 1185, 1689
MspCI	CTTAAG	1 cut(s)	1838
MspI	CCGG	7 cut(s)	41, 62, 408, 495, 2389, 2811, 2853
MunI	CAATTG	1 cut(s)	951
MvnI	CGCG	2 cut(s)	2319, 3147
MwoI	GCNNNNNNNGC	4 cut(s)	608, 1918, 3051, 3144
NarI	GGCGCC	2 cut(s)	237, 348
NciI	CCSGG	3 cut(s)	62, 2811, 2853
NcoI	CCATGG	1 cut(s)	2939
NdeI	CATATG	1 cut(s)	2584
NlaIV	GGNNCC	8 cut(s)	39, 238, 349, 687, 1007, 1999, 2296, 3151
NmeAIII	GCCGAG	1 cut(s)	211
NmuCI	GTSAC	7 cut(s)	8, 1217, 1726, 2079, 2132, 2377, 2564
NsbI	TGCGCA	1 cut(s)	875
NsiI	ATGCAT	1 cut(s)	537
NspI	RCATGY	3 cut(s)	1652, 1830, 2060
OliI	CACNNNNGTG	1 cut(s)	650
PaeI	GCATGC	1 cut(s)	2060
PagI	TCATGA	2 cut(s)	1765, 2539
PciSI	GCTCTTC	1 cut(s)	383
PcsI	WCGNNNNNNNCGW	7 cut(s)	24, 84, 87, 90, 93, 1192, 3096
PdmI	GAANNNNTTC	1 cut(s)	1335
PfeI	GAWTC	5 cut(s)	443, 491, 1582, 2284, 2578
Pfl23II	CGTACG	1 cut(s)	2685
PflFI	GACNNNGTC	1 cut(s)	80
PflMI	CCANNNNNTGG	2 cut(s)	2702, 2888
PfoI	TCCNGGA	3 cut(s)	2851, 2860, 3015
PkrI	GCNGC	3 cut(s)	739, 2820, 2871
PleI	GAGTC	6 cut(s)	387, 2030, 2129, 2368, 2548, 2604
PluTI	GGCGCC	2 cut(s)	240, 351
PmaCI	CACGTG	1 cut(s)	2111
PmlI	CACGTG	1 cut(s)	2111
PpsI	GAGTC	6 cut(s)	387, 2030, 2129, 2368, 2548, 2604
Ppu21I	YACGTR	2 cut(s)	1811, 2111
PpuMI	RGGWCCY	4 cut(s)	1006, 1834, 1976, 1997
PshAI	GACNNNNGTC	1 cut(s)	69
PshBI	ATTAAT	1 cut(s)	1061
Psp124BI	GAGCTC	1 cut(s)	2167
Psp5II	RGGWCCY	4 cut(s)	1006, 1834, 1976, 1997
PspCI	CACGTG	1 cut(s)	2111
PspEI	GGTNACC	1 cut(s)	2306
PspFI	CCCAGC	1 cut(s)	2613
PspLI	CGTACG	1 cut(s)	2685
PspN4I	GGNNCC	8 cut(s)	39, 238, 349, 687, 1007, 1999, 2296, 3151
PspPPI	RGGWCCY	4 cut(s)	1006, 1834, 1976, 1997
PsrI	GAACNNNNNNTAC	2 cut(s)	806, 838
PstNI	CAGNNNCTG	1 cut(s)	2888
PsuI	RGATCY	1 cut(s)	1204
PsyI	GACNNNGTC	1 cut(s)	80
RseI	CAYNNNNRTG	3 cut(s)	650, 1185, 1689
SacI	GAGCTC	1 cut(s)	2167
SalI	GTCGAC	2 cut(s)	83, 89
SapI	GCTCTTC	1 cut(s)	383
SaqAI	TTAA	9 cut(s)	746, 1061, 1251, 1839, 1880, 1932, 2076, 2331, 3219
SatI	GCNGC	3 cut(s)	738, 2819, 2870
ScaI	AGTACT	3 cut(s)	1297, 1568, 2767
SchI	GAGTC	6 cut(s)	388, 2031, 2129, 2369, 2548, 2605
SduI	GDGCHC	2 cut(s)	2167, 3015
SfaNI	GCATC	5 cut(s)	522, 544, 1417, 2509, 2849
SfcI	CTRYAG	1 cut(s)	2274
SfoI	GGCGCC	2 cut(s)	238, 349
SgrDI	CGTCGACG	2 cut(s)	83, 89
SmiMI	CAYNNNNRTG	3 cut(s)	650, 1185, 1689
SmlI	CTYRAG	3 cut(s)	1608, 1838, 2357
SmoI	CTYRAG	3 cut(s)	1608, 1838, 2357
SpeI	ACTAGT	1 cut(s)	2339
SphI	GCATGC	1 cut(s)	2060
SspDI	GGCGCC	2 cut(s)	236, 347
SspI	AATATT	2 cut(s)	1159, 3225
SstI	GAGCTC	1 cut(s)	2167
StyI	CCWWGG	6 cut(s)	67, 1014, 1983, 2756, 2939, 3104
TaiI	ACGT	9 cut(s)	84, 90, 183, 828, 1150, 1813, 2113, 2412, 2687
TaqII	GACCGA	3 cut(s)	141, 296, 2583
TatI	WGTACW	4 cut(s)	1295, 1566, 2765, 3073
TfiI	GAWTC	5 cut(s)	443, 491, 1582, 2284, 2578
Tru1I	TTAA	9 cut(s)	746, 1061, 1251, 1839, 1880, 1932, 2076, 2331, 3219
Tru9I	TTAA	9 cut(s)	746, 1061, 1251, 1839, 1880, 1932, 2076, 2331, 3219
TscAI	CASTG	2 cut(s)	652, 3160
TseFI	GTSAC	7 cut(s)	8, 1217, 1726, 2079, 2132, 2377, 2564
TseI	GCWGC	3 cut(s)	737, 2818, 2869
Tsp45I	GTSAC	7 cut(s)	8, 1217, 1726, 2079, 2132, 2377, 2564
TspGWI	ACGGA	4 cut(s)	925, 1679, 2695, 3197
TspRI	CASTG	2 cut(s)	652, 3160
Tth111I	GACNNNGTC	1 cut(s)	80
Van91I	CCANNNNNTGG	2 cut(s)	2702, 2888
Vha464I	CTTAAG	1 cut(s)	1838
VspI	ATTAAT	1 cut(s)	1061
XagI	CCTNNNNNAGG	4 cut(s)	1013, 2205, 2706, 2916
XapI	RAATTY	1 cut(s)	594
XbaI	TCTAGA	2 cut(s)	73, 2144
XceI	RCATGY	3 cut(s)	1652, 1830, 2060
XmiI	GTMKAC	2 cut(s)	84, 90
XmnI	GAANNNNTTC	1 cut(s)	1335
ZraI	GACGTC	2 cut(s)	82, 88
ZrmI	AGTACT	3 cut(s)	1297, 1568, 2767
Zsp2I	ATGCAT	1 cut(s)	537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.