Rmu_sc0001423.1_g000044

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001423.1
OG0004224
Physical Location & Seq
Forward (+)
203613 .. 208545
4933 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2322 bp
atgcttcttatgaaactctcacttttcttattagccgctgcaacgatattactagcagcagcagctgataaacccctcccaccgcctcaagccaagcccaactgcacacaccgctgtggtaacctcacaattccatatccctttggcatcggtgacggctgttacatgccactgaagggattcaatctcagttgtgatgattccactatatccccatcattgaacttgtccaggaccatccgtgtcactgattttaatattgctgagggtgagttgcatattttgaattttataagcaaggattgttatgacaagagcggcttgaattcgcagaacaccattcctacgctcggtttgggtcctcctttcagcatttccggtctcaaaaacaggttcgtcgccataggctgcgacacttttgctgttttcaagggttttcgtggcgcacaaccgttcatgacggggtgcctgtccgtgtgcctcaacattaacaccgtggatgaaacctcttgctctggtgccgggtgttgccagaccacaatccctgaaggactgacaaatcgtactttgacattgggtagcttttccaatcacacgaatatatgggaatttaatccctgcagctatgccttccttgtgcaagatggctactttaacttcagcggtacgaagagtttcgaagagctcaagaacagaaagaagatgccaatgatcgttaattggcagattggaaatgagccatgcgaggtagctaaaaagaatgctgaggattatgcatgcaagggaaattccacgtgtgccaaccggaccaagagcccataccctggttactattgccagtgcttgccaggctacgaagggaacccatacctcggttgccaagatattaacgagtgtttggctgaaattaaacgatgtgggaacggaacgtgccaaaatacacctggaaattacacttgtaaatgtaatgaaggattcgaaaatgatggtcaatggaagtgcattgctcgtcccaagggaaacaacacccccctgaaaatttcattgggtatttgtataggcttctgtattcttttggtcataatttttgggttatatcgcgaatacaagagaatacagttctacagattgaaaacaaagtactttgaagcaaatggcgggccaaaaatagaagctcacttcgaacaattgatgaagcaattggctagtcagaaagaacctagtcaaaaagaacctcttgggccccaaatctttactacagaacaacttaagatggcaacgaaggattacgatgaaaagcaaaaaattggcgaaggaggctatggagaagtttacaaaggcgtactcgatgataaaagagtggttgcaataaagaagtccaaaacgactgcccaggtcgaacgtgatcaatttgttactgagatcattgttctttctgatattagtcatgaaaatgtggtgaggctgctaggctgttgcgtggacacaaaaacgcctgtgctagtttacgagtttgttgataatggaactctttatgatcacattcataaaaaggaagaaaaggaattacaaccactttcatttgcacaacgattgaagatagcaacacaaactgcgacagcactttcatacttacataactccaagcaaattgttcatcgagatgtcaagttaacgaatatacttttggataagaatcttatggccaaagtgtcagactttggagcttcaaaactggttcctgacaacaaagctcaacttccaactttggtgcagggtacaatgggatacttggaccctgaatatcttcagtcccacaaactaacaaacaagagtgacgtttatagctttggagttgtcctcgttgagctactaacaagccagaatgcaattcgtttcgatacagaccaggagaagagaatcctagccaaagtattcgtttctacaattgaaaatacaggtcaacttaatcaaattcttgatggtgaaatagtcaaggatggaaattgggagatgatcgaaaaagttgctaagctcgccgaaagatgcttaagggttaaaggagtcgaaaggcctaccatggaagaagtagagagggagctaaatgaaatactgaaaacttcaaaaaagcaacaagggggaaagaagctttatgcctcctcttccaaagagacagacccctctcgtgagtcacattcaaacacttacaatgtggagattagtgatgaaggcgatgatggctctactggcattgctaccagtgcagagtattacgccagcatgcaatatcaagcccaaaagccgtccggttatggtcgataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

773

Amino Acids

85.96

Weight (kDa)

7.6

Isoelectric Point (pI)

32.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 94 5.6e-12 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 295 - 334 2.9e-06 Calcium-binding EGF domain
Pkinase PF00069 430 - 697 8.8e-53 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 433 - 701 3.5e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 293
AccB1I GGYRCC 2 cut(s) 465, 518
AccB7I CCANNNNNTGG 1 cut(s) 824
AccBSI CCGCTC 1 cut(s) 318
AccII CGCG 1 cut(s) 1101
AciI CCGC 6 cut(s) 36, 83, 112, 318, 663, 1158
AcoI YGGCCR 1 cut(s) 1704
AcsI RAATTY 6 cut(s) 286, 325, 608, 787, 1038, 1974
AcuI CTGAAG 4 cut(s) 194, 567, 643, 1793
AcvI CACGTG 1 cut(s) 795
AfaI GTAC 5 cut(s) 565, 667, 1142, 1344, 1780
AfiI CCNNNNNNNGG 4 cut(s) 176, 350, 824, 872
AflII CTTAAG 2 cut(s) 1268, 2050
AflIII ACRYGT 1 cut(s) 794
AjnI CCWGG 6 cut(s) 230, 823, 847, 943, 1392, 1908
AjuI GAANNNNNNNTTGG 4 cut(s) 1028, 1060, 1670, 1702
AleI CACNNNNGTG 1 cut(s) 114
Alw21I GWGCWC 1 cut(s) 687
Alw26I GTCTC 2 cut(s) 386, 2165
AlwNI CAGNNNCTG 1 cut(s) 65
AoxI GGCC 4 cut(s) 1160, 1241, 1704, 2072
ApaI GGGCCC 1 cut(s) 1245
ApeKI GCWGC 7 cut(s) 38, 56, 59, 62, 408, 621, 1465
ApoI RAATTY 6 cut(s) 286, 325, 608, 787, 1038, 1974
Asp700I GAANNNNTTC 3 cut(s) 179, 674, 1806
AspLEI GCGC 1 cut(s) 446
AspS9I GGNCC 7 cut(s) 234, 359, 807, 1160, 1241, 1242, 1795
AsuC2I CCSGG 1 cut(s) 523
AsuHPI GGTGA 4 cut(s) 164, 281, 1471, 1997
AsuII TTCGAA 3 cut(s) 678, 978, 1182
AvaII GGWCC 4 cut(s) 234, 359, 807, 1795
BaeGI GKGCMC 1 cut(s) 1245
BalI TGGCCA 1 cut(s) 1706
BanI GGYRCC 2 cut(s) 465, 518
BanII GRGCYC 3 cut(s) 687, 818, 1245
BauI CACGAG 1 cut(s) 2184
BbrPI CACGTG 1 cut(s) 795
Bbv12I GWGCWC 1 cut(s) 687
BbvCI CCTCAGC 2 cut(s) 264, 765
BbvI GCAGC 7 cut(s) 25, 68, 71, 74, 395, 633, 1452
BccI CCATC 8 cut(s) 223, 245, 638, 980, 1267, 1976, 1994, 2231
BceAI ACGGC 2 cut(s) 172, 2287
BcgI CGANNNNNNTGC 4 cut(s) 401, 435, 2009, 2043
BciT130I CCWGG 6 cut(s) 232, 825, 849, 945, 1394, 1910
BciVI GTATCC 1 cut(s) 1781
BclI TGATCA 2 cut(s) 1405, 1537
BcnI CCSGG 1 cut(s) 523
BcoDI GTCTC 2 cut(s) 386, 2165
BfaI CTAG 6 cut(s) 53, 1206, 1221, 1469, 1502, 1925
BfmI CTRYAG 3 cut(s) 619, 1123, 1257
BfrI CTTAAG 2 cut(s) 1268, 2050
BfuI GTATCC 1 cut(s) 1781
BisI GCNGC 9 cut(s) 36, 39, 57, 60, 63, 319, 409, 622, 1466
BlpI GCTNAGC 1 cut(s) 2031
BlsI GCNGC 9 cut(s) 37, 40, 58, 61, 64, 320, 410, 623, 1467
BmcAI AGTACT 1 cut(s) 1142
Bme1390I CCNGG 7 cut(s) 232, 523, 825, 849, 945, 1394, 1910
Bme18I GGWCC 4 cut(s) 234, 359, 807, 1795
BmgT120I GGNCC 7 cut(s) 234, 359, 807, 1160, 1241, 1242, 1795
BmiI GGNNCC 8 cut(s) 360, 467, 520, 863, 1243, 1244, 1740, 1797
BmrFI CCNGG 7 cut(s) 232, 523, 825, 849, 945, 1394, 1910
BmsI GCATC 3 cut(s) 156, 693, 2036
BplI GAGNNNNNCTC 2 cut(s) 1845, 1877
Bpu10I CCTNAGC 2 cut(s) 264, 765
Bpu1102I GCTNAGC 1 cut(s) 2031
Bpu14I TTCGAA 3 cut(s) 678, 978, 1182
BpuEI CTTGAG 2 cut(s) 72, 671
BpuMI CCSGG 1 cut(s) 523
BsaAI YACGTR 1 cut(s) 795
BsaBI GATNNNNATC 1 cut(s) 1695
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 6 cut(s) 495, 823, 871, 1014, 1392, 2079
BsaWI WCCGGW 3 cut(s) 377, 804, 2306
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 350, 824, 872
Bse1I ACTGG 4 cut(s) 838, 1740, 2251, 2259
Bse3DI GCAATG 2 cut(s) 1002, 2250
Bse8I GATNNNNATC 1 cut(s) 1695
BseBI CCWGG 6 cut(s) 232, 825, 849, 945, 1394, 1910
BseDI CCNNGG 6 cut(s) 495, 823, 871, 1014, 1392, 2079
BseGI GGATG 3 cut(s) 237, 505, 2005
BseJI GATNNNNATC 1 cut(s) 1695
BseLI CCNNNNNNNGG 4 cut(s) 176, 350, 824, 872
BseMI GCAATG 2 cut(s) 1002, 2250
BseMII CTCAG 4 cut(s) 202, 255, 756, 1410
BseNI ACTGG 4 cut(s) 838, 1740, 2251, 2259
BseRI GAGGAG 1 cut(s) 2149
BseSI GKGCMC 1 cut(s) 1245
BseXI GCAGC 7 cut(s) 25, 68, 71, 74, 395, 633, 1452
BsgI GTGCAG 3 cut(s) 88, 1793, 2283
Bsh1236I CGCG 1 cut(s) 1101
BshFI GGCC 4 cut(s) 1162, 1243, 1706, 2074
BshNI GGYRCC 2 cut(s) 465, 518
BsiHKAI GWGCWC 1 cut(s) 687
BsiSI CCGG 4 cut(s) 378, 522, 805, 2307
BslFI GGGAC 2 cut(s) 996, 1798
BslI CCNNNNNNNGG 4 cut(s) 176, 350, 824, 872
BsmAI GTCTC 2 cut(s) 386, 2165
BsmFI GGGAC 2 cut(s) 996, 1798
BsmI GAATGC 2 cut(s) 766, 1891
BsnI GGCC 4 cut(s) 1162, 1243, 1706, 2074
Bso31I GGTCTC 1 cut(s) 386
Bsp119I TTCGAA 3 cut(s) 678, 978, 1182
Bsp120I GGGCCC 1 cut(s) 1241
Bsp1286I GDGCHC 3 cut(s) 687, 818, 1245
Bsp143I GATC 5 cut(s) 711, 1405, 1422, 1537, 2016
Bsp1720I GCTNAGC 1 cut(s) 2031
Bsp19I CCATGG 1 cut(s) 2079
Bsp68I TCGCGA 1 cut(s) 1101
BspACI CCGC 6 cut(s) 36, 83, 112, 318, 663, 1158
BspANI GGCC 4 cut(s) 1162, 1243, 1706, 2074
BspCNI CTCAG 4 cut(s) 201, 256, 757, 1411
BspFNI CGCG 1 cut(s) 1101
BspHI TCATGA 2 cut(s) 456, 1447
BspLI GGNNCC 8 cut(s) 360, 467, 520, 863, 1243, 1244, 1740, 1797
BspMAI CTGCAG 1 cut(s) 623
BspQI GCTCTTC 1 cut(s) 675
BspT104I TTCGAA 3 cut(s) 678, 978, 1182
BspT107I GGYRCC 2 cut(s) 465, 518
BspTI CTTAAG 2 cut(s) 1268, 2050
BspTNI GGTCTC 1 cut(s) 386
BsrBI CCGCTC 1 cut(s) 318
BsrDI GCAATG 2 cut(s) 1002, 2250
BsrI ACTGG 4 cut(s) 838, 1740, 2251, 2259
BssECI CCNNGG 6 cut(s) 495, 823, 871, 1014, 1392, 2079
BssMI GATC 5 cut(s) 711, 1405, 1422, 1537, 2016
BssSI CACGAG 1 cut(s) 2184
BssT1I CCWWGG 2 cut(s) 1014, 2079
Bst2BI CACGAG 1 cut(s) 2184
Bst2UI CCWGG 6 cut(s) 232, 825, 849, 945, 1394, 1910
Bst4CI ACNGT 3 cut(s) 453, 496, 1119
Bst6I CTCTTC 4 cut(s) 665, 675, 1910, 2167
BstAFI CTTAAG 2 cut(s) 1268, 2050
BstBAI YACGTR 1 cut(s) 795
BstBI TTCGAA 3 cut(s) 678, 978, 1182
BstC8I GCNNGC 6 cut(s) 778, 845, 1160, 2037, 2278, 2282
BstDEI CTNAG 5 cut(s) 188, 264, 765, 1419, 2031
BstDSI CCRYGG 2 cut(s) 495, 2079
BstEII GGTNACC 1 cut(s) 119
BstF5I GGATG 3 cut(s) 237, 505, 2005
BstFNI CGCG 1 cut(s) 1101
BstHHI GCGC 1 cut(s) 446
BstKTI GATC 5 cut(s) 714, 1408, 1425, 1540, 2019
BstMAI GTCTC 2 cut(s) 386, 2165
BstMBI GATC 5 cut(s) 711, 1405, 1422, 1537, 2016
BstMWI GCNNNNNNNGC 8 cut(s) 62, 111, 849, 1317, 2036, 2238, 2247, 2261
BstNI CCWGG 6 cut(s) 232, 825, 849, 945, 1394, 1910
BstNSI RCATGY 3 cut(s) 169, 780, 2284
BstPI GGTNACC 1 cut(s) 119
BstSCI CCNGG 7 cut(s) 230, 521, 823, 847, 943, 1392, 1908
BstSFI CTRYAG 3 cut(s) 619, 1123, 1257
BstSLI GKGCMC 1 cut(s) 1245
BstUI CGCG 1 cut(s) 1101
BstV1I GCAGC 7 cut(s) 25, 68, 71, 74, 395, 633, 1452
BsuI GTATCC 1 cut(s) 1781
BsuRI GGCC 4 cut(s) 1162, 1243, 1706, 2074
BtgI CCRYGG 2 cut(s) 495, 2079
BtgZI GCGATG 1 cut(s) 2247
BtsCI GGATG 3 cut(s) 237, 505, 2005
BtsIMutI CAGTG 4 cut(s) 170, 246, 845, 2266
BtuMI TCGCGA 1 cut(s) 1101
Cac8I GCNNGC 6 cut(s) 778, 845, 1160, 2037, 2278, 2282
CaiI CAGNNNCTG 1 cut(s) 65
CciI TCATGA 2 cut(s) 456, 1447
CfoI GCGC 1 cut(s) 446
Cfr13I GGNCC 7 cut(s) 234, 359, 807, 1160, 1241, 1242, 1795
Csp6I GTAC 5 cut(s) 564, 666, 1141, 1343, 1779
CviAII CATG 7 cut(s) 166, 457, 741, 777, 1448, 2080, 2281
CviQI GTAC 5 cut(s) 564, 666, 1141, 1343, 1779
DdeI CTNAG 5 cut(s) 188, 264, 765, 1419, 2031
DpnI GATC 5 cut(s) 713, 1407, 1424, 1539, 2018
DpnII GATC 5 cut(s) 711, 1405, 1422, 1537, 2016
EaeI YGGCCR 1 cut(s) 1704
Eam1104I CTCTTC 4 cut(s) 665, 675, 1910, 2167
EarI CTCTTC 4 cut(s) 665, 675, 1910, 2167
Ecl136II GAGCTC 1 cut(s) 685
Eco130I CCWWGG 2 cut(s) 1014, 2079
Eco147I AGGCCT 1 cut(s) 2074
Eco24I GRGCYC 3 cut(s) 687, 818, 1245
Eco31I GGTCTC 1 cut(s) 386
Eco47I GGWCC 4 cut(s) 234, 359, 807, 1795
Eco53kI GAGCTC 1 cut(s) 685
Eco57I CTGAAG 4 cut(s) 194, 567, 643, 1793
Eco72I CACGTG 1 cut(s) 795
Eco91I GGTNACC 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 685
EcoO109I RGGNCCY 2 cut(s) 359, 1242
EcoO65I GGTNACC 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 325
EcoRII CCWGG 6 cut(s) 230, 823, 847, 943, 1392, 1908
EcoT14I CCWWGG 2 cut(s) 1014, 2079
EcoT22I ATGCAT 1 cut(s) 778
EcoT38I GRGCYC 3 cut(s) 687, 818, 1245
ErhI CCWWGG 2 cut(s) 1014, 2079
FaeI CATG 7 cut(s) 169, 460, 744, 780, 1451, 2083, 2284
FalI AAGNNNNNCTT 6 cut(s) 305, 337, 1221, 1253, 1743, 1775
FaqI GGGAC 2 cut(s) 996, 1798
FatI CATG 7 cut(s) 165, 456, 740, 776, 1447, 2079, 2280
FauI CCCGC 1 cut(s) 1151
FbaI TGATCA 2 cut(s) 1405, 1537
Fnu4HI GCNGC 9 cut(s) 36, 39, 57, 60, 63, 319, 409, 622, 1466
FokI GGATG 3 cut(s) 224, 512, 2012
FriOI GRGCYC 3 cut(s) 687, 818, 1245
Fsp4HI GCNGC 9 cut(s) 36, 39, 57, 60, 63, 319, 409, 622, 1466
FspBI CTAG 6 cut(s) 53, 1206, 1221, 1469, 1502, 1925
GlaI GCGC 1 cut(s) 445
GluI GCNGC 9 cut(s) 36, 39, 57, 60, 63, 319, 409, 622, 1466
HaeIII GGCC 4 cut(s) 1162, 1243, 1706, 2074
HapII CCGG 4 cut(s) 378, 522, 805, 2307
HhaI GCGC 1 cut(s) 446
Hin1II CATG 7 cut(s) 169, 460, 744, 780, 1451, 2083, 2284
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HincII GTYRAC 2 cut(s) 1674, 1964
HindII GTYRAC 2 cut(s) 1674, 1964
HindIII AAGCTT 1 cut(s) 2147
HinfI GANTC 7 cut(s) 180, 200, 975, 1696, 1920, 2064, 2189
HpaI GTTAAC 1 cut(s) 1674
HpaII CCGG 4 cut(s) 378, 522, 805, 2307
HphI GGTGA 4 cut(s) 164, 281, 1471, 1997
Hpy166II GTNNAC 5 cut(s) 1333, 1483, 1507, 1674, 1964
Hpy188I TCNGA 3 cut(s) 1212, 1438, 1717
Hpy188III TCNNGA 8 cut(s) 457, 688, 1100, 1448, 1661, 1742, 1979, 2186
Hpy8I GTNNAC 5 cut(s) 1333, 1483, 1507, 1674, 1964
Hpy99I CGWCG 1 cut(s) 401
HpyAV CCTTC 8 cut(s) 169, 542, 640, 851, 965, 1276, 1307, 2222
HpyCH4III ACNGT 3 cut(s) 453, 496, 1119
HpyCH4IV ACGT 4 cut(s) 794, 929, 1402, 1839
HpyF10VI GCNNNNNNNGC 8 cut(s) 62, 111, 849, 1317, 2036, 2238, 2247, 2261
HpyF3I CTNAG 5 cut(s) 188, 264, 765, 1419, 2031
HpySE526I ACGT 4 cut(s) 794, 929, 1402, 1839
Hsp92II CATG 7 cut(s) 169, 460, 744, 780, 1451, 2083, 2284
HspAI GCGC 1 cut(s) 444
Ksp22I TGATCA 2 cut(s) 1405, 1537
KspAI GTTAAC 1 cut(s) 1674
Kzo9I GATC 5 cut(s) 711, 1405, 1422, 1537, 2016
LguI GCTCTTC 1 cut(s) 675
LmnI GCTCC 2 cut(s) 1724, 2098
Lsp1109I GCAGC 7 cut(s) 25, 68, 71, 74, 395, 633, 1452
LweI GCATC 3 cut(s) 156, 693, 2036
MaeI CTAG 6 cut(s) 53, 1206, 1221, 1469, 1502, 1925
MaeII ACGT 4 cut(s) 794, 929, 1402, 1839
MaeIII GTNAC 8 cut(s) 119, 152, 161, 244, 827, 1414, 1835, 2190
MalI GATC 5 cut(s) 713, 1407, 1424, 1539, 2018
MbiI CCGCTC 1 cut(s) 318
MboI GATC 5 cut(s) 711, 1405, 1422, 1537, 2016
MboII GAAGA 9 cut(s) 682, 692, 712, 1568, 1609, 1799, 1927, 2096, 2154
MfeI CAATTG 3 cut(s) 1187, 1199, 1947
MhlI GDGCHC 3 cut(s) 687, 818, 1245
MlsI TGGCCA 1 cut(s) 1706
MluNI TGGCCA 1 cut(s) 1706
MlyI GAGTC 2 cut(s) 2073, 2198
MmeI TCCRAC 1 cut(s) 1787
Mox20I TGGCCA 1 cut(s) 1706
Mph1103I ATGCAT 1 cut(s) 778
MroXI GAANNNNTTC 3 cut(s) 179, 674, 1806
MscI TGGCCA 1 cut(s) 1706
MslI CAYNNNNRTG 3 cut(s) 114, 1452, 1662
Msp20I TGGCCA 1 cut(s) 1706
MspA1I CMGCKG 4 cut(s) 38, 65, 114, 663
MspCI CTTAAG 2 cut(s) 1268, 2050
MspI CCGG 4 cut(s) 378, 522, 805, 2307
MspR9I CCNGG 7 cut(s) 232, 523, 825, 849, 945, 1394, 1910
MunI CAATTG 3 cut(s) 1187, 1199, 1947
Mva1269I GAATGC 2 cut(s) 766, 1891
MvaI CCWGG 6 cut(s) 232, 825, 849, 945, 1394, 1910
MvnI CGCG 1 cut(s) 1101
MwoI GCNNNNNNNGC 8 cut(s) 62, 111, 849, 1317, 2036, 2238, 2247, 2261
NciI CCSGG 1 cut(s) 523
NcoI CCATGG 1 cut(s) 2079
NdeII GATC 5 cut(s) 711, 1405, 1422, 1537, 2016
NlaIII CATG 7 cut(s) 169, 460, 744, 780, 1451, 2083, 2284
NlaIV GGNNCC 8 cut(s) 360, 467, 520, 863, 1243, 1244, 1740, 1797
NmuCI GTSAC 4 cut(s) 152, 244, 1835, 2190
NruI TCGCGA 1 cut(s) 1101
NsiI ATGCAT 1 cut(s) 778
NspI RCATGY 3 cut(s) 169, 780, 2284
NspV TTCGAA 3 cut(s) 678, 978, 1182
OliI CACNNNNGTG 1 cut(s) 114
PaeI GCATGC 2 cut(s) 780, 2284
PagI TCATGA 2 cut(s) 456, 1447
PceI AGGCCT 1 cut(s) 2074
PciSI GCTCTTC 1 cut(s) 675
PctI GAATGC 2 cut(s) 766, 1891
PdmI GAANNNNTTC 3 cut(s) 179, 674, 1806
PfeI GAWTC 5 cut(s) 180, 200, 975, 1696, 1920
PflMI CCANNNNNTGG 1 cut(s) 824
PfoI TCCNGGA 1 cut(s) 230
PkrI GCNGC 9 cut(s) 37, 40, 58, 61, 64, 320, 410, 623, 1467
PleI GAGTC 2 cut(s) 2072, 2197
PmaCI CACGTG 1 cut(s) 795
PmlI CACGTG 1 cut(s) 795
PpsI GAGTC 2 cut(s) 2072, 2197
Ppu21I YACGTR 1 cut(s) 795
PpuMI RGGWCCY 1 cut(s) 359
PsiI TTATAA 1 cut(s) 293
Psp124BI GAGCTC 1 cut(s) 687
Psp5II RGGWCCY 1 cut(s) 359
Psp6I CCWGG 6 cut(s) 230, 823, 847, 943, 1392, 1908
PspCI CACGTG 1 cut(s) 795
PspEI GGTNACC 1 cut(s) 119
PspGI CCWGG 6 cut(s) 230, 823, 847, 943, 1392, 1908
PspN4I GGNNCC 8 cut(s) 360, 467, 520, 863, 1243, 1244, 1740, 1797
PspOMI GGGCCC 1 cut(s) 1241
PspPI GGNCC 7 cut(s) 234, 359, 807, 1160, 1241, 1242, 1795
PspPPI RGGWCCY 1 cut(s) 359
PstI CTGCAG 1 cut(s) 623
PstNI CAGNNNCTG 1 cut(s) 65
PvuII CAGCTG 1 cut(s) 65
RruI TCGCGA 1 cut(s) 1101
RsaI GTAC 5 cut(s) 565, 667, 1142, 1344, 1780
RsaNI GTAC 5 cut(s) 564, 666, 1141, 1343, 1779
RseI CAYNNNNRTG 3 cut(s) 114, 1452, 1662
SacI GAGCTC 1 cut(s) 687
SapI GCTCTTC 1 cut(s) 675
SatI GCNGC 9 cut(s) 36, 39, 57, 60, 63, 319, 409, 622, 1466
Sau3AI GATC 5 cut(s) 711, 1405, 1422, 1537, 2016
Sau96I GGNCC 7 cut(s) 234, 359, 807, 1160, 1241, 1242, 1795
ScaI AGTACT 1 cut(s) 1142
SchI GAGTC 2 cut(s) 2073, 2198
ScrFI CCNGG 7 cut(s) 232, 523, 825, 849, 945, 1394, 1910
SduI GDGCHC 3 cut(s) 687, 818, 1245
SfaNI GCATC 3 cut(s) 156, 693, 2036
SfcI CTRYAG 3 cut(s) 619, 1123, 1257
SfuI TTCGAA 3 cut(s) 678, 978, 1182
SinI GGWCC 4 cut(s) 234, 359, 807, 1795
SmiMI CAYNNNNRTG 3 cut(s) 114, 1452, 1662
SmlI CTYRAG 4 cut(s) 87, 686, 1268, 2050
SmoI CTYRAG 4 cut(s) 87, 686, 1268, 2050
SphI GCATGC 2 cut(s) 780, 2284
SseBI AGGCCT 1 cut(s) 2074
SsiI CCGC 6 cut(s) 36, 83, 112, 318, 663, 1158
SspI AATATT 1 cut(s) 259
SspMI CTAG 6 cut(s) 53, 1206, 1221, 1469, 1502, 1925
SstI GAGCTC 1 cut(s) 687
StuI AGGCCT 1 cut(s) 2074
StyD4I CCNGG 7 cut(s) 230, 521, 823, 847, 943, 1392, 1908
StyI CCWWGG 2 cut(s) 1014, 2079
TaaI ACNGT 3 cut(s) 453, 496, 1119
TaiI ACGT 4 cut(s) 797, 932, 1405, 1842
TatI WGTACW 1 cut(s) 1140
TauI GCSGC 2 cut(s) 38, 321
TfiI GAWTC 5 cut(s) 180, 200, 975, 1696, 1920
TscAI CASTG 4 cut(s) 177, 253, 845, 2266
TseFI GTSAC 4 cut(s) 152, 244, 1835, 2190
TseI GCWGC 7 cut(s) 38, 56, 59, 62, 408, 621, 1465
Tsp45I GTSAC 4 cut(s) 152, 244, 1835, 2190
TspGWI ACGGA 3 cut(s) 230, 463, 939
TspRI CASTG 4 cut(s) 177, 253, 845, 2266
Van91I CCANNNNNTGG 1 cut(s) 824
Vha464I CTTAAG 2 cut(s) 1268, 2050
VpaK11BI GGWCC 4 cut(s) 234, 359, 807, 1795
XapI RAATTY 6 cut(s) 286, 325, 608, 787, 1038, 1974
XceI RCATGY 3 cut(s) 169, 780, 2284
XcmI CCANNNNNNNNNTGG 1 cut(s) 941
XmnI GAANNNNTTC 3 cut(s) 179, 674, 1806
XspI CTAG 6 cut(s) 53, 1206, 1221, 1469, 1502, 1925
ZrmI AGTACT 1 cut(s) 1142
Zsp2I ATGCAT 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.