Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0002268.1

Orthogroup ID

OG0002914

Physical Location & Seq

Genomic Coordinates Reverse (-)

106300 .. 111217

Gene Structure 4918 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2163 bp

atgcacggatacggcattttgccttcgtatcccgtatcgatccgggatatgggtacgatacgccccggatacgcccggatacgtctaccacctacagagttctcatctacttcgtttcgcttggagcttcaatcaaactcgctccggagggatatgtctggtggtgaaggatctgacttagaggattatccatcaatcagagacacagactcggaagatgaagagagtggttcctctgaaaatgagggtgagctcaacaccagcagaaatgatggtttggccattgatcatggtaatgaggaggaaagtgatagttctggacttcatgaagcagccgaggaaagtgattcatctgaagatgaggttgctccccgaaatacaattggtgctgttcccttggagtggtatcgggatgagaaacatattggttatgatcttacagggaagaagattaaaaagaaggaaagagaagataaattacaatcctttcttgctagtgctgatgattccaaaaattggcgcaaatttttggatgaatacaatgacgaggaagtagaagtgacaaaagaagatatgaaaactataaatagattgcttgaaggaaaggcgccacacggtgactttgatccatatgcgccttatgttgattggtttaaatgggatgactcaaagcatccactgtcaaatgcccctgaacccaagagacgattcattccttcaaaatctgaaagtaaactggtgaagaggttagtattagcaattcggaagggtttaattaagcctacaaaggatgaagaagaggaagaagaaagtgtttatcccttgtggggagacgactctaattcaacggaaaaggatggtagccatttatcttacattccagcgccaaagccaaaattgccagggtatgaggagtccttcaatcctcctccggaattcatcccaacacaagaagagattgatgcttatcagttaatgtatgaggaagaccgccctaagtttataccgaaaaggtttacatctatgagaagcatccctgcatatgagaatgctattaaggagtgcttcgaaagatgtttggatctatacttgtgccccagagttcggaagaaacgtatcaatattgatcctgaatctctgaagcccaagctaccaagccgaaaggatctcaagccttatcctgtgaaatgttatctagagtatagaggtcacaaagacacagttacatcagtttccacagaagcttcagggcagtggattgcatcaggttcatcggatggaactgtgcgtatttgggaggttgaaactggtagatgtctcaaactctgggagattggagaagctgtcacatatgtagcttggaatcccaatcctgagtattccgtattggctgtctctgtgggacaagatgtacttattctcaacactggttttgggaatgaagaagtacagaaaaaaactaaagaacttctttcggctgaaagacctaccacaccagatgacacggctaccttcgtgagctggattcaagatgaaacacatgagggcaccaggttaagacattttaagactgtgtcttcagtggaatggcatcgtagaggagactacttttcaacggtgaagtctacccagacattttcaatcaagttgggtgggcttgcagttacttcagttttccatcctgttcgctcccacatcttcatttctacaaagaaaactattcgccaatatgatttggtgaagggaaagcaaatcagaaagcttgatacaggactaaaagaaatctcctccattgcagttcatccttctggtgatcatgtaattgtggggagcagagaaggaaaattttgttggtttgatatggacctttcaaccaaaaaaccttaccttattcgccagtggcataagaaggacatcaacaatgtgtctatccatcgtttatatccgttgtttgcaacatgctctgatgattgcactgtacaagtttctcatggcatggtttattcagatcttaaccagaaccctcttatagttccactggaaatactccgaggtcatgcaagctcagatgggagaggtgtaatggattgcaaattccacccaaggcagccctggttgttcacggccggtgctgactcggtgatcagactttactgcgattga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000463 GO:0000470 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0030684 GO:0030686 GO:0030687 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042273 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0044877 GO:0046483 GO:0070013 GO:0070545 GO:0071704 GO:0071840 GO:0090304 GO:1901360 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BOP1NT WD40

Protein Analysis

720

Amino Acids

82.06

Weight (kDa)

5.55

Isoelectric Point (pI)

46.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BOP1NT	PF08145	136 - 394	3.8e-100	BOP1NT (NUC169) domain
WD40_CDC20-Fz	PF24807	391 - 466	3.4e-06	CDC20/Fizzy WD40 domain
WD40	PF00400	396 - 432	9.6e-10	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	397 - 476	1.2e-12	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	399 - 533	4.1e-11	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	399 - 470	4.4e-08	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	399 - 481	1.1e-10	WDHD1 first WD40 domain
WD40_Prp19	PF24814	400 - 463	4.5e-06	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	400 - 461	1.2e-06	WDR36/Utp21 second beta-propeller domain
EIF3I	PF24805	401 - 465	5.3e-09	EIF3I
WD40_CDC20-Fz	PF24807	543 - 667	8.1e-07	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	559 - 718	4.9e-11	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	567 - 718	2.1e-08	WDR3 first beta-propeller domain
Beta-prop_EML_2	PF23414	568 - 662	3.4e-07	Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19	PF24814	572 - 718	8.4e-09	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	593 - 718	1.3e-06	WDR5 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002914)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40360 AT2G40360
fragaria_vesca	FvH4_5g21650 FvH4_5g21650 FvH4_5g21650
malus_domestica	MD05G1062400.v1.1 MD10G1070100.v1.1
prunus_persica	Prupe.6G114000_v2.0.a1 Prupe.7G053600_v2.0.a1 Prupe.7G053600_v2.0.a1 Prupe.7G053600_v2.0.a1
pyrus_communis	pycom05g05370 pycom05g05390 pycom10g05570
rosa_chinensis	RchiOBHm_Chr7g0209131 RchiOBHm_Chr7g0241521 RchiOBHm_Chr7g0241531 RchiOBHm_Chr7g0241541
rosa_laevigata	RLG00000000568
rosa_multiflora	Rmu_sc0000821.1_g000025 Rmu_sc0002268.1_g000023
rosa_roxburghii	Rroxscaffold_3G00218950 Rroxscaffold_3G00249700
rosa_rugosa	Rorug07G0109700 Rorug07G0109800 Rorug07G0345000
rosa_samantha	Rh7AG243600 Rh7AG499500 Rh7AG499600 Rh7BG238700 Rh7BG470600 Rh7BG470700 Rh7CG259100 Rh7DG250600 Rh7DG484000 Rh7DG484200
rosa_wichuraiana	Rw7G020660 Rw7G042200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	607, 1547
AccB7I	CCANNNNNTGG	1 cut(s)	516
AccI	GTMKAC	2 cut(s)	85, 1625
AccIII	TCCGGA	2 cut(s)	144, 931
AciI	CCGC	1 cut(s)	991
AclWI	GGATC	6 cut(s)	34, 178, 620, 1089, 1121, 1173
AcoI	YGGCCR	2 cut(s)	279, 2124
AcsI	RAATTY	4 cut(s)	524, 935, 1844, 2093
AcuI	CTGAAG	5 cut(s)	375, 1160, 1230, 1563, 1653
AcyI	GRCGYC	1 cut(s)	608
AdeI	CACNNNGTG	1 cut(s)	617
AfaI	GTAC	4 cut(s)	55, 1413, 1449, 1980
AfiI	CCNNNNNNNGG	5 cut(s)	49, 402, 516, 827, 1104
AjnI	CCWGG	3 cut(s)	901, 1550, 2111
AjuI	GAANNNNNNNTTGG	4 cut(s)	692, 724, 1834, 1866
AluBI	AGCT	9 cut(s)	127, 253, 1150, 1244, 1343, 1358, 1521, 1762, 2064
AluI	AGCT	9 cut(s)	127, 253, 1150, 1244, 1343, 1358, 1521, 1762, 2064
Alw21I	GWGCWC	1 cut(s)	255
Alw26I	GTCTC	6 cut(s)	195, 697, 825, 1322, 1399, 1596
AlwI	GGATC	6 cut(s)	34, 178, 620, 1089, 1121, 1173
Aor13HI	TCCGGA	2 cut(s)	144, 931
AoxI	GGCC	2 cut(s)	279, 2124
ApeKI	GCWGC	2 cut(s)	332, 2107
ApoI	RAATTY	4 cut(s)	524, 935, 1844, 2093
AspLEI	GCGC	4 cut(s)	522, 610, 637, 886
AspS9I	GGNCC	1 cut(s)	1864
AsuC2I	CCSGG	3 cut(s)	44, 66, 76
AsuHPI	GGTGA	8 cut(s)	176, 260, 629, 751, 1630, 1750, 1823, 2152
AsuII	TTCGAA	1 cut(s)	1068
AvaII	GGWCC	1 cut(s)	1864
BaeGI	GKGCMC	2 cut(s)	1097, 1550
BalI	TGGCCA	1 cut(s)	281
BanI	GGYRCC	2 cut(s)	607, 1547
BanII	GRGCYC	1 cut(s)	255
BarI	GAAGNNNNNNTAC	2 cut(s)	462, 494
BbsI	GAAGAC	2 cut(s)	993, 1569
Bbv12I	GWGCWC	1 cut(s)	255
BbvI	GCAGC	2 cut(s)	344, 2119
BccI	CCATC	7 cut(s)	199, 266, 851, 1271, 1686, 1941, 2063
BceAI	ACGGC	3 cut(s)	28, 1521, 2139
BciT130I	CCWGG	3 cut(s)	903, 1552, 2113
BciVI	GTATCC	3 cut(s)	39, 62, 72
BclI	TGATCA	3 cut(s)	286, 1813, 2142
BcnI	CCSGG	3 cut(s)	44, 66, 76
BcoDI	GTCTC	6 cut(s)	195, 697, 825, 1322, 1399, 1596
BfaI	CTAG	2 cut(s)	495, 1196
BfmI	CTRYAG	1 cut(s)	93
BfoI	RGCGCY	2 cut(s)	611, 887
BfuI	GTATCC	3 cut(s)	39, 62, 72
BglII	AGATCT	1 cut(s)	2008
BisI	GCNGC	2 cut(s)	333, 2108
BlsI	GCNGC	2 cut(s)	334, 2109
Bme1390I	CCNGG	6 cut(s)	44, 66, 76, 903, 1552, 2113
Bme18I	GGWCC	1 cut(s)	1864
BmgT120I	GGNCC	1 cut(s)	1864
BmiI	GGNNCC	3 cut(s)	232, 609, 1549
BmrFI	CCNGG	6 cut(s)	44, 66, 76, 903, 1552, 2113
BmsI	GCATC	5 cut(s)	682, 952, 1041, 1271, 1600
BpiI	GAAGAC	2 cut(s)	993, 1569
Bpu14I	TTCGAA	1 cut(s)	1068
BpuEI	CTTGAG	1 cut(s)	1154
BpuMI	CCSGG	3 cut(s)	44, 66, 76
Bsa29I	ATCGAT	1 cut(s)	38
BsaBI	GATNNNNATC	1 cut(s)	966
BsaHI	GRCGYC	1 cut(s)	608
BsaJI	CCNNGG	7 cut(s)	64, 336, 396, 902, 2050, 2102, 2111
BsaWI	WCCGGW	2 cut(s)	144, 931
BsaXI	ACNNNNNCTCC	2 cut(s)	1329, 1359
Bsc4I	CCNNNNNNNGG	5 cut(s)	49, 402, 516, 827, 1104
Bse118I	RCCGGY	1 cut(s)	2126
Bse1I	ACTGG	5 cut(s)	741, 1312, 1432, 1897, 2043
Bse3DI	GCAATG	1 cut(s)	1791
Bse8I	GATNNNNATC	1 cut(s)	966
BseAI	TCCGGA	2 cut(s)	144, 931
BseBI	CCWGG	3 cut(s)	903, 1552, 2113
BseCI	ATCGAT	1 cut(s)	38
BseDI	CCNNGG	7 cut(s)	64, 336, 396, 902, 2050, 2102, 2111
BseJI	GATNNNNATC	1 cut(s)	966
BseLI	CCNNNNNNNGG	5 cut(s)	49, 402, 516, 827, 1104
BseMI	GCAATG	1 cut(s)	1791
BseMII	CTCAG	2 cut(s)	1365, 2079
BseNI	ACTGG	5 cut(s)	741, 1312, 1432, 1897, 2043
BseRI	GAGGAG	5 cut(s)	314, 918, 926, 1614, 1777
BseSI	GKGCMC	2 cut(s)	1097, 1550
BseX3I	CGGCCG	1 cut(s)	2124
BseXI	GCAGC	2 cut(s)	344, 2119
Bsh1285I	CGRYCG	1 cut(s)	2127
BshFI	GGCC	2 cut(s)	281, 2126
BshNI	GGYRCC	2 cut(s)	607, 1547
BshVI	ATCGAT	1 cut(s)	38
BsiEI	CGRYCG	1 cut(s)	2127
BsiHKAI	GWGCWC	1 cut(s)	255
BsiSI	CCGG	6 cut(s)	43, 66, 76, 145, 932, 2127
BslFI	GGGAC	1 cut(s)	1416
BslI	CCNNNNNNNGG	5 cut(s)	49, 402, 516, 827, 1104
BsmAI	GTCTC	6 cut(s)	195, 697, 825, 1322, 1399, 1596
BsmBI	CGTCTC	2 cut(s)	697, 825
BsmFI	GGGAC	1 cut(s)	1416
BsmI	GAATGC	1 cut(s)	1054
BsnI	GGCC	2 cut(s)	281, 2126
Bsp119I	TTCGAA	1 cut(s)	1068
Bsp1286I	GDGCHC	3 cut(s)	255, 1097, 1550
Bsp13I	TCCGGA	2 cut(s)	144, 931
Bsp1407I	TGTACA	1 cut(s)	1978
BspACI	CCGC	1 cut(s)	991
BspANI	GGCC	2 cut(s)	281, 2126
BspCNI	CTCAG	2 cut(s)	1366, 2078
BspDI	ATCGAT	1 cut(s)	38
BspEI	TCCGGA	2 cut(s)	144, 931
BspHI	TCATGA	1 cut(s)	325
BspLI	GGNNCC	3 cut(s)	232, 609, 1549
BspPI	GGATC	6 cut(s)	34, 178, 620, 1089, 1121, 1173
BspT104I	TTCGAA	1 cut(s)	1068
BspT107I	GGYRCC	2 cut(s)	607, 1547
BsrDI	GCAATG	1 cut(s)	1791
BsrFI	RCCGGY	1 cut(s)	2126
BsrGI	TGTACA	1 cut(s)	1978
BsrI	ACTGG	5 cut(s)	741, 1312, 1432, 1897, 2043
BssAI	RCCGGY	1 cut(s)	2126
BssECI	CCNNGG	7 cut(s)	64, 336, 396, 902, 2050, 2102, 2111
BssNI	GRCGYC	1 cut(s)	608
BssT1I	CCWWGG	2 cut(s)	396, 2102
Bst2UI	CCWGG	3 cut(s)	903, 1552, 2113
Bst4CI	ACNGT	7 cut(s)	617, 681, 1222, 1285, 1573, 1618, 1978
Bst6I	CTCTTC	4 cut(s)	216, 737, 792, 948
BstACI	GRCGYC	1 cut(s)	608
BstAUI	TGTACA	1 cut(s)	1978
BstBI	TTCGAA	1 cut(s)	1068
BstC8I	GCNNGC	2 cut(s)	1659, 2062
BstDEI	CTNAG	4 cut(s)	178, 996, 1374, 2065
BstH2I	RGCGCY	2 cut(s)	611, 887
BstHHI	GCGC	4 cut(s)	522, 610, 637, 886
BstMAI	GTCTC	6 cut(s)	195, 697, 825, 1322, 1399, 1596
BstMCI	CGRYCG	1 cut(s)	2127
BstMWI	GCNNNNNNNGC	1 cut(s)	898
BstNI	CCWGG	3 cut(s)	903, 1552, 2113
BstNSI	RCATGY	1 cut(s)	1962
BstSCI	CCNGG	6 cut(s)	42, 64, 74, 901, 1550, 2111
BstSFI	CTRYAG	1 cut(s)	93
BstSLI	GKGCMC	2 cut(s)	1097, 1550
BstV1I	GCAGC	2 cut(s)	344, 2119
BstV2I	GAAGAC	2 cut(s)	993, 1569
BstX2I	RGATCY	4 cut(s)	170, 1081, 1165, 2008
BstYI	RGATCY	4 cut(s)	170, 1081, 1165, 2008
BstZI	CGGCCG	1 cut(s)	2124
Bsu15I	ATCGAT	1 cut(s)	38
BsuI	GTATCC	3 cut(s)	39, 62, 72
BsuRI	GGCC	2 cut(s)	281, 2126
BsuTUI	ATCGAT	1 cut(s)	38
BtsI	GCAGTG	1 cut(s)	1259
BtsIMutI	CAGTG	7 cut(s)	677, 1259, 1425, 1587, 1904, 1974, 2036
Cac8I	GCNNGC	2 cut(s)	1659, 2062
CciI	TCATGA	1 cut(s)	325
CfoI	GCGC	4 cut(s)	522, 610, 637, 886
Cfr10I	RCCGGY	1 cut(s)	2126
Cfr13I	GGNCC	1 cut(s)	1864
ClaI	ATCGAT	1 cut(s)	38
CsiI	ACCWGGT	1 cut(s)	1550
Csp6I	GTAC	4 cut(s)	54, 1412, 1448, 1979
CspCI	CAANNNNNGTGG	2 cut(s)	1633, 1668
CviAII	CATG	8 cut(s)	290, 326, 1541, 1817, 1959, 1991, 1996, 2057
CviQI	GTAC	4 cut(s)	54, 1412, 1448, 1979
DdeI	CTNAG	4 cut(s)	178, 996, 1374, 2065
DinI	GGCGCC	1 cut(s)	609
DraI	TTTAAA	1 cut(s)	655
DraIII	CACNNNGTG	1 cut(s)	617
EaeI	YGGCCR	2 cut(s)	279, 2124
EagI	CGGCCG	1 cut(s)	2124
Eam1104I	CTCTTC	4 cut(s)	216, 737, 792, 948
EarI	CTCTTC	4 cut(s)	216, 737, 792, 948
Ecl136II	GAGCTC	1 cut(s)	253
EclXI	CGGCCG	1 cut(s)	2124
Eco130I	CCWWGG	2 cut(s)	396, 2102
Eco24I	GRGCYC	1 cut(s)	255
Eco47I	GGWCC	1 cut(s)	1864
Eco52I	CGGCCG	1 cut(s)	2124
Eco53kI	GAGCTC	1 cut(s)	253
Eco57I	CTGAAG	5 cut(s)	375, 1160, 1230, 1563, 1653
EcoICRI	GAGCTC	1 cut(s)	253
EcoRI	GAATTC	1 cut(s)	935
EcoRII	CCWGG	3 cut(s)	901, 1550, 2111
EcoT14I	CCWWGG	2 cut(s)	396, 2102
EcoT38I	GRGCYC	1 cut(s)	255
EgeI	GGCGCC	1 cut(s)	609
EheI	GGCGCC	1 cut(s)	609
ErhI	CCWWGG	2 cut(s)	396, 2102
Esp3I	CGTCTC	2 cut(s)	697, 825
FaeI	CATG	8 cut(s)	293, 329, 1544, 1820, 1962, 1994, 1999, 2060
FalI	AAGNNNNNCTT	6 cut(s)	1049, 1081, 1398, 1430, 1455, 1487
FaqI	GGGAC	1 cut(s)	1416
FatI	CATG	8 cut(s)	289, 325, 1540, 1816, 1958, 1990, 1995, 2056
FauNDI	CATATG	3 cut(s)	631, 1042, 1351
FbaI	TGATCA	3 cut(s)	286, 1813, 2142
FblI	GTMKAC	2 cut(s)	85, 1625
Fnu4HI	GCNGC	2 cut(s)	333, 2108
FriOI	GRGCYC	1 cut(s)	255
Fsp4HI	GCNGC	2 cut(s)	333, 2108
FspBI	CTAG	2 cut(s)	495, 1196
GlaI	GCGC	4 cut(s)	521, 609, 636, 885
GluI	GCNGC	2 cut(s)	333, 2108
HaeII	RGCGCY	2 cut(s)	611, 887
HaeIII	GGCC	2 cut(s)	281, 2126
HapII	CCGG	6 cut(s)	43, 66, 76, 145, 932, 2127
HhaI	GCGC	4 cut(s)	522, 610, 637, 886
Hin1I	GRCGYC	1 cut(s)	608
Hin1II	CATG	8 cut(s)	293, 329, 1544, 1820, 1962, 1994, 1999, 2060
Hin6I	GCGC	4 cut(s)	520, 608, 635, 884
HinP1I	GCGC	4 cut(s)	520, 608, 635, 884
HindIII	AAGCTT	2 cut(s)	1242, 1760
HpaII	CCGG	6 cut(s)	43, 66, 76, 145, 932, 2127
HphI	GGTGA	8 cut(s)	176, 260, 629, 751, 1630, 1750, 1823, 2152
Hpy166II	GTNNAC	5 cut(s)	86, 734, 1017, 1626, 2121
Hpy8I	GTNNAC	5 cut(s)	86, 734, 1017, 1626, 2121
HpyCH4III	ACNGT	7 cut(s)	617, 681, 1222, 1285, 1573, 1618, 1978
HpyCH4IV	ACGT	2 cut(s)	82, 1114
HpyCH4V	TGCA	9 cut(s)	4, 1040, 1262, 1661, 1796, 1955, 1974, 2060, 2091
HpyF10VI	GCNNNNNNNGC	1 cut(s)	898
HpyF3I	CTNAG	4 cut(s)	178, 996, 1374, 2065
HpySE526I	ACGT	2 cut(s)	82, 1114
Hsp92I	GRCGYC	1 cut(s)	608
Hsp92II	CATG	8 cut(s)	293, 329, 1544, 1820, 1962, 1994, 1999, 2060
HspAI	GCGC	4 cut(s)	520, 608, 635, 884
KasI	GGCGCC	1 cut(s)	607
Kpn2I	TCCGGA	2 cut(s)	144, 931
Ksp22I	TGATCA	3 cut(s)	286, 1813, 2142
LmnI	GCTCC	5 cut(s)	124, 147, 373, 1694, 1830
Lsp1109I	GCAGC	2 cut(s)	344, 2119
LweI	GCATC	5 cut(s)	682, 952, 1041, 1271, 1600
MabI	ACCWGGT	1 cut(s)	1550
MaeI	CTAG	2 cut(s)	495, 1196
MaeII	ACGT	2 cut(s)	82, 1114
MaeIII	GTNAC	6 cut(s)	559, 617, 1208, 1222, 1345, 1663
MfeI	CAATTG	1 cut(s)	381
MflI	RGATCY	4 cut(s)	170, 1081, 1165, 2008
MhlI	GDGCHC	3 cut(s)	255, 1097, 1550
MlsI	TGGCCA	1 cut(s)	281
MluNI	TGGCCA	1 cut(s)	281
Mly113I	GGCGCC	1 cut(s)	608
MlyI	GAGTC	5 cut(s)	203, 659, 830, 923, 2129
Mox20I	TGGCCA	1 cut(s)	281
MroI	TCCGGA	2 cut(s)	144, 931
MscI	TGGCCA	1 cut(s)	281
MseI	TTAA	9 cut(s)	453, 654, 773, 777, 974, 1056, 1556, 1566, 2013
MslI	CAYNNNNRTG	1 cut(s)	294
Msp20I	TGGCCA	1 cut(s)	281
MspI	CCGG	6 cut(s)	43, 66, 76, 145, 932, 2127
MspR9I	CCNGG	6 cut(s)	44, 66, 76, 903, 1552, 2113
MunI	CAATTG	1 cut(s)	381
Mva1269I	GAATGC	1 cut(s)	1054
MvaI	CCWGG	3 cut(s)	903, 1552, 2113
MwoI	GCNNNNNNNGC	1 cut(s)	898
NarI	GGCGCC	1 cut(s)	608
NciI	CCSGG	3 cut(s)	44, 66, 76
NdeI	CATATG	3 cut(s)	631, 1042, 1351
NlaIII	CATG	8 cut(s)	293, 329, 1544, 1820, 1962, 1994, 1999, 2060
NlaIV	GGNNCC	3 cut(s)	232, 609, 1549
NmeAIII	GCCGAG	1 cut(s)	361
NmuCI	GTSAC	4 cut(s)	559, 617, 1208, 1345
NspI	RCATGY	1 cut(s)	1962
NspV	TTCGAA	1 cut(s)	1068
PacI	TTAATTAA	1 cut(s)	777
PagI	TCATGA	1 cut(s)	325
PcsI	WCGNNNNNNNCGW	1 cut(s)	1111
PctI	GAATGC	1 cut(s)	1054
PfeI	GAWTC	6 cut(s)	347, 506, 708, 1133, 1363, 1525
PflFI	GACNNNGTC	1 cut(s)	1573
PflMI	CCANNNNNTGG	1 cut(s)	516
PfoI	TCCNGGA	1 cut(s)	42
PkrI	GCNGC	2 cut(s)	334, 2109
PleI	GAGTC	5 cut(s)	203, 659, 830, 922, 2129
PluTI	GGCGCC	1 cut(s)	611
PpsI	GAGTC	5 cut(s)	203, 659, 830, 922, 2129
Psp124BI	GAGCTC	1 cut(s)	255
Psp6I	CCWGG	3 cut(s)	901, 1550, 2111
PspGI	CCWGG	3 cut(s)	901, 1550, 2111
PspN4I	GGNNCC	3 cut(s)	232, 609, 1549
PspPI	GGNCC	1 cut(s)	1864
PsuI	RGATCY	4 cut(s)	170, 1081, 1165, 2008
PsyI	GACNNNGTC	1 cut(s)	1573
RsaI	GTAC	4 cut(s)	55, 1413, 1449, 1980
RsaNI	GTAC	4 cut(s)	54, 1412, 1448, 1979
RseI	CAYNNNNRTG	1 cut(s)	294
SacI	GAGCTC	1 cut(s)	255
SaqAI	TTAA	9 cut(s)	453, 654, 773, 777, 974, 1056, 1556, 1566, 2013
SatI	GCNGC	2 cut(s)	333, 2108
Sau96I	GGNCC	1 cut(s)	1864
SchI	GAGTC	5 cut(s)	203, 659, 830, 923, 2129
ScrFI	CCNGG	6 cut(s)	44, 66, 76, 903, 1552, 2113
SduI	GDGCHC	3 cut(s)	255, 1097, 1550
SexAI	ACCWGGT	1 cut(s)	1550
SfaNI	GCATC	5 cut(s)	682, 952, 1041, 1271, 1600
SfcI	CTRYAG	1 cut(s)	93
SfoI	GGCGCC	1 cut(s)	609
SfuI	TTCGAA	1 cut(s)	1068
SinI	GGWCC	1 cut(s)	1864
SmiMI	CAYNNNNRTG	1 cut(s)	294
SmlI	CTYRAG	1 cut(s)	1169
SmoI	CTYRAG	1 cut(s)	1169
SsiI	CCGC	1 cut(s)	991
SspDI	GGCGCC	1 cut(s)	607
SspI	AATATT	1 cut(s)	1123
SspMI	CTAG	2 cut(s)	495, 1196
SstI	GAGCTC	1 cut(s)	255
StyD4I	CCNGG	6 cut(s)	42, 64, 74, 901, 1550, 2111
StyI	CCWWGG	2 cut(s)	396, 2102
TaaI	ACNGT	7 cut(s)	617, 681, 1222, 1285, 1573, 1618, 1978
TaiI	ACGT	2 cut(s)	85, 1117
TaqI	TCGA	2 cut(s)	38, 1068
TatI	WGTACW	3 cut(s)	1411, 1447, 1978
TfiI	GAWTC	6 cut(s)	347, 506, 708, 1133, 1363, 1525
Tru1I	TTAA	9 cut(s)	453, 654, 773, 777, 974, 1056, 1556, 1566, 2013
Tru9I	TTAA	9 cut(s)	453, 654, 773, 777, 974, 1056, 1556, 1566, 2013
TscAI	CASTG	7 cut(s)	684, 1259, 1432, 1587, 1904, 1981, 2043
TseFI	GTSAC	4 cut(s)	559, 617, 1208, 1345
TseI	GCWGC	2 cut(s)	332, 2107
Tsp45I	GTSAC	4 cut(s)	559, 617, 1208, 1345
TspGWI	ACGGA	4 cut(s)	21, 863, 1372, 1935
TspRI	CASTG	7 cut(s)	684, 1259, 1432, 1587, 1904, 1981, 2043
Tth111I	GACNNNGTC	1 cut(s)	1573
Van91I	CCANNNNNTGG	1 cut(s)	516
VpaK11BI	GGWCC	1 cut(s)	1864
XapI	RAATTY	4 cut(s)	524, 935, 1844, 2093
XbaI	TCTAGA	1 cut(s)	1195
XceI	RCATGY	1 cut(s)	1962
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2109
XmiI	GTMKAC	2 cut(s)	85, 1625
XspI	CTAG	2 cut(s)	495, 1196

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.