Rmu_sc0002723.1_g000041

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002723.1
OG0002071
Physical Location & Seq
Reverse (-)
131088 .. 132332
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
atgtcgccgatttgtagccgagtgaagaggtcccgaataaagaagatgagattctatttatccgacccggaactcggatgtgataaaatgatggaggaacgcggcggatctgagcagcaagtcgaggtcctggagttggttggtgagggacgtggcagcgctacagagtggagaggaggttgggctggagagtggagaagaggggattctcggcgacggagaggcctcaaacccaacctcaaccgtccatccacctcgctccaatcaatcacagacgtccatgacctcctccccaagtacggcctcccaaggggtctgttacccgacaacgtaaggtcctacactctctccgacaatggaaccttcgagatctatctcgagaacccatgttatgttcactttgatcagttggtgtactacaacaagaacattaaagggaagctgagtttcggctctgtttccaatgtgtcggggattcaagccaagaagctattttatttgggttaccgtgacggggatgcatatggaacaagggtctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

179

Amino Acids

20.41

Weight (kDa)

9.46

Isoelectric Point (pI)

53.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 93 - 166 2.9e-20 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
AccII CGCG 1 cut(s) 102
AciI CCGC 2 cut(s) 102, 105
AclWI GGATC 1 cut(s) 115
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 2 cut(s) 299, 416
AfeI AGCGCT 1 cut(s) 160
AfiI CCNNNNNNNGG 5 cut(s) 74, 136, 299, 310, 514
AgsI TTSAA 1 cut(s) 479
AjiI CACGTC 1 cut(s) 152
AjnI CCWGG 1 cut(s) 129
AluBI AGCT 2 cut(s) 442, 490
AluI AGCT 2 cut(s) 442, 490
AlwI GGATC 1 cut(s) 115
Ama87I CYCGRG 1 cut(s) 377
Aor51HI AGCGCT 1 cut(s) 160
AoxI GGCC 2 cut(s) 223, 301
ApeKI GCWGC 2 cut(s) 115, 156
AspLEI GCGC 1 cut(s) 161
AspS9I GGNCC 3 cut(s) 30, 127, 336
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 3 cut(s) 30, 127, 336
BbvI GCAGC 2 cut(s) 127, 168
BccI CCATC 2 cut(s) 85, 256
BceAI ACGGC 1 cut(s) 316
BciT130I CCWGG 1 cut(s) 131
BclI TGATCA 1 cut(s) 403
BcnI CCSGG 1 cut(s) 68
BfmI CTRYAG 1 cut(s) 162
BfoI RGCGCY 1 cut(s) 162
BglII AGATCT 1 cut(s) 369
BisI GCNGC 3 cut(s) 103, 116, 157
BlsI GCNGC 3 cut(s) 104, 117, 158
Bme1390I CCNGG 2 cut(s) 68, 131
Bme18I GGWCC 3 cut(s) 30, 127, 336
BmeT110I CYCGRG 1 cut(s) 377
BmgBI CACGTC 1 cut(s) 152
BmgT120I GGNCC 3 cut(s) 30, 127, 336
BmiI GGNNCC 2 cut(s) 32, 361
BmrFI CCNGG 2 cut(s) 68, 131
BmsI GCATC 1 cut(s) 508
BpmI CTGGAG 2 cut(s) 152, 207
BpuMI CCSGG 1 cut(s) 68
BsaHI GRCGYC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 308
BsaXI ACNNNNNCTCC 4 cut(s) 163, 193, 332, 362
Bsc4I CCNNNNNNNGG 5 cut(s) 74, 136, 299, 310, 514
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 1 cut(s) 308
BseGI GGATG 3 cut(s) 83, 248, 523
BseLI CCNNNNNNNGG 5 cut(s) 74, 136, 299, 310, 514
BseMII CTCAG 2 cut(s) 102, 434
BseRI GAGGAG 2 cut(s) 189, 278
BseXI GCAGC 2 cut(s) 127, 168
Bsh1236I CGCG 1 cut(s) 102
BshFI GGCC 2 cut(s) 225, 303
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 1 cut(s) 68
BslFI GGGAC 2 cut(s) 16, 162
BslI CCNNNNNNNGG 5 cut(s) 74, 136, 299, 310, 514
BsmFI GGGAC 2 cut(s) 16, 162
BsnI GGCC 2 cut(s) 225, 303
BsoBI CYCGRG 1 cut(s) 377
Bsp143I GATC 3 cut(s) 107, 369, 403
BspACI CCGC 2 cut(s) 102, 105
BspANI GGCC 2 cut(s) 225, 303
BspCNI CTCAG 2 cut(s) 103, 435
BspFNI CGCG 1 cut(s) 102
BspLI GGNNCC 2 cut(s) 32, 361
BspPI GGATC 1 cut(s) 115
BssECI CCNNGG 1 cut(s) 308
BssMI GATC 3 cut(s) 107, 369, 403
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 1 cut(s) 308
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 2 cut(s) 245, 509
Bst6I CTCTTC 2 cut(s) 20, 193
BstACI GRCGYC 1 cut(s) 276
BstDEI CTNAG 2 cut(s) 111, 443
BstEII GGTNACC 1 cut(s) 503
BstF5I GGATG 3 cut(s) 83, 248, 523
BstFNI CGCG 1 cut(s) 102
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 3 cut(s) 110, 372, 406
BstMBI GATC 3 cut(s) 107, 369, 403
BstNI CCWGG 1 cut(s) 131
BstPI GGTNACC 1 cut(s) 503
BstSCI CCNGG 2 cut(s) 66, 129
BstSFI CTRYAG 1 cut(s) 162
BstUI CGCG 1 cut(s) 102
BstV1I GCAGC 2 cut(s) 127, 168
BstX2I RGATCY 2 cut(s) 107, 369
BstYI RGATCY 2 cut(s) 107, 369
BsuRI GGCC 2 cut(s) 225, 303
BtrI CACGTC 1 cut(s) 152
BtsCI GGATG 3 cut(s) 83, 248, 523
CfoI GCGC 1 cut(s) 161
Cfr13I GGNCC 3 cut(s) 30, 127, 336
Csp6I GTAC 2 cut(s) 298, 415
CviAII CATG 2 cut(s) 281, 387
CviJI RGCY 8 cut(s) 18, 185, 225, 303, 442, 453, 482, 490
CviKI_1 RGCY 8 cut(s) 18, 185, 225, 303, 442, 453, 482, 490
CviQI GTAC 2 cut(s) 298, 415
DdeI CTNAG 2 cut(s) 111, 443
DpnI GATC 3 cut(s) 109, 371, 405
DpnII GATC 3 cut(s) 107, 369, 403
Eam1104I CTCTTC 2 cut(s) 20, 193
EarI CTCTTC 2 cut(s) 20, 193
EciI GGCGGA 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 308
Eco147I AGGCCT 1 cut(s) 225
Eco47I GGWCC 3 cut(s) 30, 127, 336
Eco47III AGCGCT 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 377
Eco91I GGTNACC 1 cut(s) 503
EcoO109I RGGNCCY 3 cut(s) 30, 127, 336
EcoO65I GGTNACC 1 cut(s) 503
EcoRII CCWGG 1 cut(s) 129
EcoT14I CCWWGG 1 cut(s) 308
EcoT22I ATGCAT 1 cut(s) 523
ErhI CCWWGG 1 cut(s) 308
FaeI CATG 2 cut(s) 284, 390
FaiI YATR 5 cut(s) 282, 388, 393, 523, 525
FaqI GGGAC 2 cut(s) 16, 162
FatI CATG 2 cut(s) 280, 386
FauNDI CATATG 1 cut(s) 523
FbaI TGATCA 1 cut(s) 403
Fnu4HI GCNGC 3 cut(s) 103, 116, 157
FokI GGATG 3 cut(s) 90, 235, 530
Fsp4HI GCNGC 3 cut(s) 103, 116, 157
GlaI GCGC 1 cut(s) 160
GluI GCNGC 3 cut(s) 103, 116, 157
GsuI CTGGAG 2 cut(s) 152, 207
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 2 cut(s) 225, 303
HapII CCGG 1 cut(s) 68
HhaI GCGC 1 cut(s) 161
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 2 cut(s) 284, 390
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HinfI GANTC 3 cut(s) 51, 206, 475
HpaII CCGG 1 cut(s) 68
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 2 cut(s) 397, 415
Hpy188I TCNGA 5 cut(s) 64, 77, 112, 352, 539
Hpy188III TCNNGA 4 cut(s) 33, 367, 377, 379
Hpy8I GTNNAC 2 cut(s) 397, 415
Hpy99I CGWCG 1 cut(s) 219
HpyAV CCTTC 1 cut(s) 373
HpyCH4III ACNGT 2 cut(s) 245, 509
HpyCH4IV ACGT 3 cut(s) 151, 276, 330
HpyCH4V TGCA 1 cut(s) 521
HpyF3I CTNAG 2 cut(s) 111, 443
HpySE526I ACGT 3 cut(s) 151, 276, 330
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 2 cut(s) 284, 390
HspAI GCGC 1 cut(s) 159
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 3 cut(s) 107, 369, 403
LmnI GCTCC 1 cut(s) 264
LpnPI CCDG 4 cut(s) 81, 116, 143, 171
Lsp1109I GCAGC 2 cut(s) 127, 168
LweI GCATC 1 cut(s) 508
MaeII ACGT 3 cut(s) 151, 276, 330
MaeIII GTNAC 3 cut(s) 318, 503, 509
MalI GATC 3 cut(s) 109, 371, 405
MboI GATC 3 cut(s) 107, 369, 403
MboII GAAGA 3 cut(s) 37, 55, 210
MflI RGATCY 2 cut(s) 107, 369
MmeI TCCRAC 2 cut(s) 87, 375
Mph1103I ATGCAT 1 cut(s) 523
MseI TTAA 1 cut(s) 432
MspI CCGG 1 cut(s) 68
MspR9I CCNGG 2 cut(s) 68, 131
MvaI CCWGG 1 cut(s) 131
MvnI CGCG 1 cut(s) 102
NciI CCSGG 1 cut(s) 68
NdeI CATATG 1 cut(s) 523
NdeII GATC 3 cut(s) 107, 369, 403
NlaIII CATG 2 cut(s) 284, 390
NlaIV GGNNCC 2 cut(s) 32, 361
NmeAIII GCCGAG 2 cut(s) 44, 190
NmuCI GTSAC 1 cut(s) 509
NsiI ATGCAT 1 cut(s) 523
PaeR7I CTCGAG 1 cut(s) 377
PceI AGGCCT 1 cut(s) 225
PfeI GAWTC 3 cut(s) 51, 206, 475
PfoI TCCNGGA 1 cut(s) 129
PkrI GCNGC 3 cut(s) 104, 117, 158
PpuMI RGGWCCY 3 cut(s) 30, 127, 336
Psp5II RGGWCCY 3 cut(s) 30, 127, 336
Psp6I CCWGG 1 cut(s) 129
PspEI GGTNACC 1 cut(s) 503
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 2 cut(s) 32, 361
PspPI GGNCC 3 cut(s) 30, 127, 336
PspPPI RGGWCCY 3 cut(s) 30, 127, 336
PsuI RGATCY 2 cut(s) 107, 369
RsaI GTAC 2 cut(s) 299, 416
RsaNI GTAC 2 cut(s) 298, 415
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 3 cut(s) 103, 116, 157
Sau3AI GATC 3 cut(s) 107, 369, 403
Sau96I GGNCC 3 cut(s) 30, 127, 336
ScrFI CCNGG 2 cut(s) 68, 131
SfaNI GCATC 1 cut(s) 508
SfcI CTRYAG 1 cut(s) 162
Sfr274I CTCGAG 1 cut(s) 377
SinI GGWCC 3 cut(s) 30, 127, 336
SlaI CTCGAG 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SseBI AGGCCT 1 cut(s) 225
SsiI CCGC 2 cut(s) 102, 105
StuI AGGCCT 1 cut(s) 225
StyD4I CCNGG 2 cut(s) 66, 129
StyI CCWWGG 1 cut(s) 308
TaaI ACNGT 2 cut(s) 245, 509
TaiI ACGT 3 cut(s) 154, 279, 333
TaqI TCGA 3 cut(s) 123, 366, 378
TatI WGTACW 1 cut(s) 414
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 3 cut(s) 51, 206, 475
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 2 cut(s) 115, 156
Tsp45I GTSAC 1 cut(s) 509
TspGWI ACGGA 1 cut(s) 232
VpaK11BI GGWCC 3 cut(s) 30, 127, 336
XhoI CTCGAG 1 cut(s) 377
ZraI GACGTC 1 cut(s) 277
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.