Rmu_sc0003872.1_g000004

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003872.1
OG0002883
Physical Location & Seq
Forward (+)
8102 .. 10401
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1152 bp
atgggacagatggttgatgaagataagaaatggacgattacatttgcgagagagttgaagaaggagggtattatagctgcaccaatggttgcagtgacggtactgcagtatcttctgcaactagtatcgatgattatggtcggacatcttgatcagctctcgctatccagtgtagcaattgccatttctctcaccaatgtcactggtttcagccttcttgcacgcaaatattcaatgtggcttattcctgcactatttggtgcttcaattattaaaccgttaactcgctacttccagtctcagagtctcattttcccaatgctcctatcctctctcgccattcttctcttccatatatctgcctcatgggcttttgtgtataagctgggatttggaagtaaaggttcagcaatagccttcagtttatccacttggttatatgtggtaatgctgggttcctatgcaatgtattcctcagtcttcgagaaaacccgtattacaatttcaaaagattctattcttcatgttggagagttctttcgcttggctatcccgtctgctgtaatggtttgtactcgggtagcaaatgaattaggagctggtaatccacaagcagctcgagtagctgttcaggctgcagtgtttctctcagtcactgaagcgcttattgtaagcactactctcttttgcggtcgttctgttttgggacgtgctttcagcagtgagaagcaggtagtggactatgttgcagttatggcacctctgatttgcctctcagtttttatggacagcttacaagcagtactctcaggtacatacctagctatgttcgaaatgcttgtcttatttagaatacattatctcaccataagaacacatgtctataaaggggtagctagaggaagtggatggcagcatttgggggcgtatgtcaatcttggggcattttatttggttggacttcctgtggctatggtactcgggtttcctgtgcacttaagggggaaaggcctttggattggaatagtggtcggctcggttgtgcaatcaactcttctgggttgcatcacctatttcacaaattggactaaacaggttcataactctattttagatcatatcaaccaattcattttacatgaacttacataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

383

Amino Acids

42.09

Weight (kDa)

9.44

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 192 - 281 3.6e-11 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 721
AccB1I GGYRCC 1 cut(s) 757
AciI CCGC 1 cut(s) 690
AcuI CTGAAG 2 cut(s) 403, 678
AfaI GTAC 5 cut(s) 102, 574, 804, 814, 978
AfeI AGCGCT 1 cut(s) 663
AflII CTTAAG 1 cut(s) 997
AflIII ACRYGT 1 cut(s) 877
AgsI TTSAA 4 cut(s) 58, 234, 267, 507
AhlI ACTAGT 1 cut(s) 121
AjiI CACGTC 1 cut(s) 710
AluBI AGCT 9 cut(s) 77, 157, 385, 599, 617, 626, 792, 824, 896
AluI AGCT 9 cut(s) 77, 157, 385, 599, 617, 626, 792, 824, 896
Alw21I GWGCWC 1 cut(s) 996
Alw26I GTCTC 2 cut(s) 303, 311
Alw44I GTGCAC 1 cut(s) 992
AlwNI CAGNNNCTG 1 cut(s) 656
Ama87I CYCGRG 3 cut(s) 576, 618, 980
Aor51HI AGCGCT 1 cut(s) 663
AoxI GGCC 1 cut(s) 1009
ApaLI GTGCAC 1 cut(s) 992
ApeKI GCWGC 4 cut(s) 77, 614, 635, 913
ArsI GACNNNNNNTTYG 2 cut(s) 825, 857
AspLEI GCGC 1 cut(s) 664
AsuHPI GGTGA 3 cut(s) 184, 856, 1060
AsuII TTCGAA 1 cut(s) 831
AvaI CYCGRG 3 cut(s) 576, 618, 980
BaeGI GKGCMC 1 cut(s) 996
BaeI ACNNNNGTAYC 6 cut(s) 92, 92, 125, 125, 968, 1001
BanI GGYRCC 1 cut(s) 757
BbsI GAAGAC 1 cut(s) 472
Bbv12I GWGCWC 1 cut(s) 996
BbvI GCAGC 4 cut(s) 64, 622, 626, 925
BccI CCATC 2 cut(s) 4, 903
BclI TGATCA 1 cut(s) 151
BcoDI GTCTC 2 cut(s) 303, 311
BcuI ACTAGT 1 cut(s) 121
BfaI CTAG 3 cut(s) 122, 821, 897
BfmI CTRYAG 2 cut(s) 104, 636
BfoI RGCGCY 1 cut(s) 665
BfrI CTTAAG 1 cut(s) 997
BfuAI ACCTGC 1 cut(s) 721
BglI GCCNNNNNGGC 1 cut(s) 368
BisI GCNGC 4 cut(s) 78, 615, 636, 914
BlsI GCNGC 4 cut(s) 79, 616, 637, 915
BmcAI AGTACT 1 cut(s) 804
BmeT110I CYCGRG 3 cut(s) 576, 618, 980
BmgBI CACGTC 1 cut(s) 710
BmiI GGNNCC 2 cut(s) 457, 759
BmsI GCATC 1 cut(s) 1074
BpiI GAAGAC 1 cut(s) 472
Bpu14I TTCGAA 1 cut(s) 831
Bsa29I ATCGAT 1 cut(s) 128
Bse1I ACTGG 3 cut(s) 168, 208, 295
Bse3DI GCAATG 1 cut(s) 471
BseCI ATCGAT 1 cut(s) 128
BseGI GGATG 1 cut(s) 914
BseMI GCAATG 1 cut(s) 471
BseMII CTCAG 5 cut(s) 314, 489, 663, 789, 822
BseNI ACTGG 3 cut(s) 168, 208, 295
BseSI GKGCMC 1 cut(s) 996
BseXI GCAGC 4 cut(s) 64, 622, 626, 925
BseYI CCCAGC 2 cut(s) 385, 451
BsgI GTGCAG 2 cut(s) 63, 234
Bsh1285I CGRYCG 1 cut(s) 694
BshFI GGCC 1 cut(s) 1011
BshNI GGYRCC 1 cut(s) 757
BshVI ATCGAT 1 cut(s) 128
BsiEI CGRYCG 1 cut(s) 694
BsiHKAI GWGCWC 1 cut(s) 996
BsiHKCI CYCGRG 3 cut(s) 576, 618, 980
BslFI GGGAC 2 cut(s) 18, 720
BsmAI GTCTC 2 cut(s) 303, 311
BsmFI GGGAC 2 cut(s) 18, 720
BsnI GGCC 1 cut(s) 1011
BsoBI CYCGRG 3 cut(s) 576, 618, 980
Bsp119I TTCGAA 1 cut(s) 831
Bsp1286I GDGCHC 1 cut(s) 996
Bsp143I GATC 2 cut(s) 151, 1114
BspACI CCGC 1 cut(s) 690
BspANI GGCC 1 cut(s) 1011
BspCNI CTCAG 5 cut(s) 313, 488, 662, 788, 821
BspDI ATCGAT 1 cut(s) 128
BspLI GGNNCC 2 cut(s) 457, 759
BspMAI CTGCAG 2 cut(s) 108, 640
BspMI ACCTGC 1 cut(s) 721
BspT104I TTCGAA 1 cut(s) 831
BspT107I GGYRCC 1 cut(s) 757
BspTI CTTAAG 1 cut(s) 997
BsrDI GCAATG 1 cut(s) 471
BsrI ACTGG 3 cut(s) 168, 208, 295
BssMI GATC 2 cut(s) 151, 1114
Bst4CI ACNGT 2 cut(s) 100, 279
Bst6I CTCTTC 2 cut(s) 353, 1059
BstAFI CTTAAG 1 cut(s) 997
BstBI TTCGAA 1 cut(s) 831
BstC8I GCNNGC 1 cut(s) 223
BstDEI CTNAG 5 cut(s) 300, 475, 649, 775, 808
BstF5I GGATG 1 cut(s) 914
BstH2I RGCGCY 1 cut(s) 665
BstHHI GCGC 1 cut(s) 664
BstKTI GATC 2 cut(s) 154, 1117
BstMAI GTCTC 2 cut(s) 303, 311
BstMBI GATC 2 cut(s) 151, 1114
BstMCI CGRYCG 1 cut(s) 694
BstMWI GCNNNNNNNGC 4 cut(s) 368, 623, 632, 755
BstNSI RCATGY 1 cut(s) 881
BstSFI CTRYAG 2 cut(s) 104, 636
BstSLI GKGCMC 1 cut(s) 996
BstV1I GCAGC 4 cut(s) 64, 622, 626, 925
BstV2I GAAGAC 1 cut(s) 472
Bsu15I ATCGAT 1 cut(s) 128
BsuRI GGCC 1 cut(s) 1011
BsuTUI ATCGAT 1 cut(s) 128
BtrI CACGTC 1 cut(s) 710
BtsCI GGATG 1 cut(s) 914
BtsI GCAGTG 3 cut(s) 99, 645, 727
BtsIMutI CAGTG 6 cut(s) 99, 175, 201, 645, 654, 727
BveI ACCTGC 1 cut(s) 721
Cac8I GCNNGC 1 cut(s) 223
CaiI CAGNNNCTG 1 cut(s) 656
CfoI GCGC 1 cut(s) 664
ClaI ATCGAT 1 cut(s) 128
Csp6I GTAC 5 cut(s) 101, 573, 803, 813, 977
CviAII CATG 4 cut(s) 366, 524, 878, 1139
CviQI GTAC 5 cut(s) 101, 573, 803, 813, 977
DdeI CTNAG 5 cut(s) 300, 475, 649, 775, 808
DpnI GATC 2 cut(s) 153, 1116
DpnII GATC 2 cut(s) 151, 1114
Eam1104I CTCTTC 2 cut(s) 353, 1059
EarI CTCTTC 2 cut(s) 353, 1059
Eco147I AGGCCT 1 cut(s) 1011
Eco47III AGCGCT 1 cut(s) 663
Eco57I CTGAAG 2 cut(s) 403, 678
Eco88I CYCGRG 3 cut(s) 576, 618, 980
FaeI CATG 4 cut(s) 369, 527, 881, 1142
FaqI GGGAC 2 cut(s) 18, 720
FatI CATG 4 cut(s) 365, 523, 877, 1138
FbaI TGATCA 1 cut(s) 151
Fnu4HI GCNGC 4 cut(s) 78, 615, 636, 914
FokI GGATG 1 cut(s) 921
Fsp4HI GCNGC 4 cut(s) 78, 615, 636, 914
FspBI CTAG 3 cut(s) 122, 821, 897
GlaI GCGC 1 cut(s) 663
GluI GCNGC 4 cut(s) 78, 615, 636, 914
GsaI CCCAGC 2 cut(s) 389, 455
HaeII RGCGCY 1 cut(s) 665
HaeIII GGCC 1 cut(s) 1011
HhaI GCGC 1 cut(s) 664
Hin1II CATG 4 cut(s) 369, 527, 881, 1142
Hin6I GCGC 1 cut(s) 662
HinP1I GCGC 1 cut(s) 662
HincII GTYRAC 1 cut(s) 282
HindII GTYRAC 1 cut(s) 282
HinfI GANTC 2 cut(s) 304, 512
HpaI GTTAAC 1 cut(s) 282
HphI GGTGA 3 cut(s) 184, 856, 1060
Hpy166II GTNNAC 3 cut(s) 282, 739, 994
Hpy188I TCNGA 3 cut(s) 143, 303, 765
Hpy188III TCNNGA 2 cut(s) 149, 484
Hpy8I GTNNAC 3 cut(s) 282, 739, 994
HpyAV CCTTC 3 cut(s) 55, 224, 427
HpyCH4III ACNGT 2 cut(s) 100, 279
HpyCH4IV ACGT 1 cut(s) 709
HpyF10VI GCNNNNNNNGC 4 cut(s) 368, 623, 632, 755
HpyF3I CTNAG 5 cut(s) 300, 475, 649, 775, 808
HpySE526I ACGT 1 cut(s) 709
Hsp92II CATG 4 cut(s) 369, 527, 881, 1142
HspAI GCGC 1 cut(s) 662
Ksp22I TGATCA 1 cut(s) 151
KspAI GTTAAC 1 cut(s) 282
Kzo9I GATC 2 cut(s) 151, 1114
LmnI GCTCC 2 cut(s) 327, 596
Lsp1109I GCAGC 4 cut(s) 64, 622, 626, 925
LweI GCATC 1 cut(s) 1074
MaeI CTAG 3 cut(s) 122, 821, 897
MaeII ACGT 1 cut(s) 709
MaeIII GTNAC 3 cut(s) 94, 199, 652
MalI GATC 2 cut(s) 153, 1116
MboI GATC 2 cut(s) 151, 1114
MboII GAAGA 8 cut(s) 32, 70, 104, 335, 340, 472, 512, 1046
MfeI CAATTG 1 cut(s) 177
MhlI GDGCHC 1 cut(s) 996
MluCI AATT 6 cut(s) 177, 267, 501, 590, 1081, 1127
MlyI GAGTC 1 cut(s) 313
MmeI TCCRAC 3 cut(s) 121, 508, 937
MnlI CCTC 7 cut(s) 58, 340, 373, 484, 771, 782, 893
MseI TTAA 3 cut(s) 273, 281, 998
MspCI CTTAAG 1 cut(s) 997
MunI CAATTG 1 cut(s) 177
MwoI GCNNNNNNNGC 4 cut(s) 368, 623, 632, 755
NdeII GATC 2 cut(s) 151, 1114
NlaIII CATG 4 cut(s) 369, 527, 881, 1142
NlaIV GGNNCC 2 cut(s) 457, 759
NmuCI GTSAC 3 cut(s) 94, 199, 652
NspI RCATGY 1 cut(s) 881
NspV TTCGAA 1 cut(s) 831
PaeR7I CTCGAG 1 cut(s) 618
PceI AGGCCT 1 cut(s) 1011
PciI ACATGT 1 cut(s) 877
PfeI GAWTC 1 cut(s) 512
PkrI GCNGC 4 cut(s) 79, 616, 637, 915
PleI GAGTC 1 cut(s) 312
PpsI GAGTC 1 cut(s) 312
PscI ACATGT 1 cut(s) 877
PspFI CCCAGC 2 cut(s) 385, 451
PspN4I GGNNCC 2 cut(s) 457, 759
PspXI VCTCGAGB 1 cut(s) 618
PstI CTGCAG 2 cut(s) 108, 640
PstNI CAGNNNCTG 1 cut(s) 656
RsaI GTAC 5 cut(s) 102, 574, 804, 814, 978
RsaNI GTAC 5 cut(s) 101, 573, 803, 813, 977
SaqAI TTAA 3 cut(s) 273, 281, 998
SatI GCNGC 4 cut(s) 78, 615, 636, 914
Sau3AI GATC 2 cut(s) 151, 1114
ScaI AGTACT 1 cut(s) 804
SchI GAGTC 1 cut(s) 313
SduI GDGCHC 1 cut(s) 996
SfaNI GCATC 1 cut(s) 1074
SfcI CTRYAG 2 cut(s) 104, 636
Sfr274I CTCGAG 1 cut(s) 618
SfuI TTCGAA 1 cut(s) 831
SlaI CTCGAG 1 cut(s) 618
SmlI CTYRAG 2 cut(s) 618, 997
SmoI CTYRAG 2 cut(s) 618, 997
SpeI ACTAGT 1 cut(s) 121
Sse9I AATT 6 cut(s) 177, 267, 501, 590, 1081, 1127
SseBI AGGCCT 1 cut(s) 1011
SsiI CCGC 1 cut(s) 690
SspI AATATT 1 cut(s) 230
SspMI CTAG 3 cut(s) 122, 821, 897
StuI AGGCCT 1 cut(s) 1011
TaaI ACNGT 2 cut(s) 100, 279
TaiI ACGT 1 cut(s) 712
TaqI TCGA 4 cut(s) 128, 483, 619, 831
TasI AATT 6 cut(s) 177, 267, 501, 590, 1081, 1127
TatI WGTACW 2 cut(s) 572, 802
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 3 cut(s) 273, 281, 998
Tru9I TTAA 3 cut(s) 273, 281, 998
TscAI CASTG 6 cut(s) 99, 175, 208, 645, 661, 727
TseFI GTSAC 3 cut(s) 94, 199, 652
TseI GCWGC 4 cut(s) 77, 614, 635, 913
Tsp45I GTSAC 3 cut(s) 94, 199, 652
TspDTI ATGAA 5 cut(s) 33, 512, 603, 1088, 1120
TspRI CASTG 6 cut(s) 99, 175, 208, 645, 661, 727
Vha464I CTTAAG 1 cut(s) 997
VneI GTGCAC 1 cut(s) 992
XceI RCATGY 1 cut(s) 881
XhoI CTCGAG 1 cut(s) 618
XspI CTAG 3 cut(s) 122, 821, 897
ZrmI AGTACT 1 cut(s) 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.