Rmu_sc0004336.1_g000040

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004336.1
OG0004248
Physical Location & Seq
Forward (+)
149538 .. 155223
5686 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1197 bp
atgtggagtcaccgaaaccctcaaggcagcaccaataggcaagccggaggaagcaagggtttcgccaaaacccaaaaggtctttgtcccaaaaaatcaaggccagaatcgtcccagaagccctaaatccccaaattccactctctctagctctcttcggcaatcgctgtcccagccatccaatgccgaagtttcaagtgctgctgcttctgcgctgccggagagtagtagtagtagtagtagggttcggatgggagagagaggcgagtgggtgtcgaccaagggaagttttgtgaattacttgccccaagatgaggcggtttccgctagccttggcgccaatgaaggtggattagatgacatggagtctcagagagtcgtcgatcttctcaatagagagctttctcgattgctcaagcaaaatcctaaggaattttggaaacaagatgtttcaaaaaagaagaagaaaaataagagcaagaaaaagaaatcacaggcagagcatactgatccacaatctacaactaggcaaactgctccacaatctacaactaggcaaactgatccaccatccattcctcttattgatctgttcccacatggggagtttcctgagggcgaaattcaacaatacagagatgacaacttgtggaggactacttctgaagagaagagggagttggagcgccttgaaaaaccattatataattcagttcgtcgagctgcagaagttcatcgccaggtacggaaatacatcagagggattttaaagcctggaatgttgatgactgatttgtgtgagactttggagaacacagtccgtaagttaatatccgagaatggcctggaagcaggcattgcattccccacaggatgctctttgaattgggttgctgctcattggaccccaaatacaggggacaagatggtgcttcagtatgatgatgtgatgaaattagactttggaactcatgttgatggtcatatagttgattgtgcatttacagtagcattcaatcctatgtttgatccactacttgaagcctcacgtgaagcaacaaatacgggtatcaaggagtctggaattgatgtgcgtctttgtgttgttggtgctgcaatccaagaagtcatggaatcatatgaggttgaaataaatgggaaggtatatcaaggtaattttcctaatgatatgagatag

Protein Analysis

398

Amino Acids

44.44

Weight (kDa)

8.7

Isoelectric Point (pI)

35.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M24 PF00557 238 - 382 6.4e-18 Metallopeptidase family M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 335
AccI GTMKAC 1 cut(s) 275
AciI CCGC 2 cut(s) 317, 324
AclWI GGATC 3 cut(s) 503, 557, 1022
AcsI RAATTY 3 cut(s) 133, 431, 621
AcuI CTGAAG 2 cut(s) 684, 917
AcvI CACGTG 1 cut(s) 1049
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 1 cut(s) 744
AfiI CCNNNNNNNGG 3 cut(s) 313, 601, 914
AgsI TTSAA 8 cut(s) 195, 453, 626, 692, 883, 1015, 1040, 1148
AhdI GACNNNNNGTC 1 cut(s) 364
AjnI CCWGG 3 cut(s) 738, 772, 843
AjuI GAANNNNNNNTTGG 2 cut(s) 662, 694
AluBI AGCT 3 cut(s) 150, 400, 722
AluI AGCT 3 cut(s) 150, 400, 722
Alw26I GTCTC 2 cut(s) 372, 794
AlwI GGATC 3 cut(s) 503, 557, 1022
AoxI GGCC 2 cut(s) 100, 841
ApeKI GCWGC 7 cut(s) 27, 200, 203, 214, 722, 893, 1112
ApoI RAATTY 3 cut(s) 133, 431, 621
AspLEI GCGC 3 cut(s) 214, 338, 687
AspS9I GGNCC 1 cut(s) 903
AsuNHI GCTAGC 1 cut(s) 326
AvaII GGWCC 1 cut(s) 903
AxyI CCTNAGG 2 cut(s) 426, 612
BanI GGYRCC 1 cut(s) 335
BbrPI CACGTG 1 cut(s) 1049
BbvI GCAGC 7 cut(s) 39, 187, 190, 201, 709, 880, 1099
BccI CCATC 5 cut(s) 184, 244, 577, 919, 971
BcgI CGANNNNNNTGC 2 cut(s) 43, 77
BciT130I CCWGG 3 cut(s) 740, 774, 845
BcoDI GTCTC 2 cut(s) 372, 794
BfaI CTAG 4 cut(s) 147, 327, 525, 552
BfmI CTRYAG 1 cut(s) 723
BfoI RGCGCY 2 cut(s) 339, 688
BisI GCNGC 7 cut(s) 28, 201, 204, 215, 723, 894, 1113
BlsI GCNGC 7 cut(s) 29, 202, 205, 216, 724, 895, 1114
Bme1390I CCNGG 3 cut(s) 740, 774, 845
Bme18I GGWCC 1 cut(s) 903
BmeRI GACNNNNNGTC 1 cut(s) 364
BmgT120I GGNCC 1 cut(s) 903
BmiI GGNNCC 2 cut(s) 337, 905
BmrFI CCNGG 3 cut(s) 740, 774, 845
BmsI GCATC 1 cut(s) 863
BmtI GCTAGC 1 cut(s) 330
BpuEI CTTGAG 2 cut(s) 6, 398
BsaAI YACGTR 1 cut(s) 1049
BsaHI GRCGYC 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 279, 331
Bsc4I CCNNNNNNNGG 3 cut(s) 313, 601, 914
Bse21I CCTNAGG 2 cut(s) 426, 612
Bse3DI GCAATG 1 cut(s) 855
BseBI CCWGG 3 cut(s) 740, 774, 845
BseDI CCNNGG 2 cut(s) 279, 331
BseGI GGATG 4 cut(s) 176, 255, 569, 878
BseLI CCNNNNNNNGG 3 cut(s) 313, 601, 914
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 2 cut(s) 383, 603
BseXI GCAGC 7 cut(s) 39, 187, 190, 201, 709, 880, 1099
BseYI CCCAGC 1 cut(s) 171
BshFI GGCC 2 cut(s) 102, 843
BshNI GGYRCC 1 cut(s) 335
BsiSI CCGG 2 cut(s) 45, 218
BslFI GGGAC 4 cut(s) 71, 96, 154, 932
BslI CCNNNNNNNGG 3 cut(s) 313, 601, 914
BsmAI GTCTC 2 cut(s) 372, 794
BsmFI GGGAC 4 cut(s) 71, 96, 154, 932
BsmI GAATGC 2 cut(s) 860, 1010
BsnI GGCC 2 cut(s) 102, 843
Bsp143I GATC 5 cut(s) 382, 508, 562, 586, 1027
BspACI CCGC 2 cut(s) 317, 324
BspANI GGCC 2 cut(s) 102, 843
BspCNI CTCAG 2 cut(s) 382, 604
BspLI GGNNCC 2 cut(s) 337, 905
BspMAI CTGCAG 1 cut(s) 727
BspOI GCTAGC 1 cut(s) 330
BspPI GGATC 3 cut(s) 503, 557, 1022
BspT107I GGYRCC 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 855
BssECI CCNNGG 2 cut(s) 279, 331
BssMI GATC 5 cut(s) 382, 508, 562, 586, 1027
BssNI GRCGYC 1 cut(s) 336
BssT1I CCWWGG 2 cut(s) 279, 331
Bst2UI CCWGG 3 cut(s) 740, 774, 845
Bst4CI ACNGT 2 cut(s) 817, 1006
Bst6I CTCTTC 3 cut(s) 159, 660, 665
BstACI GRCGYC 1 cut(s) 336
BstAPI GCANNNNNTGC 1 cut(s) 857
BstBAI YACGTR 1 cut(s) 1049
BstC8I GCNNGC 3 cut(s) 42, 328, 853
BstDEI CTNAG 3 cut(s) 369, 426, 612
BstF5I GGATG 4 cut(s) 176, 255, 569, 878
BstH2I RGCGCY 2 cut(s) 339, 688
BstHHI GCGC 3 cut(s) 214, 338, 687
BstKTI GATC 5 cut(s) 385, 511, 565, 589, 1030
BstMAI GTCTC 2 cut(s) 372, 794
BstMBI GATC 5 cut(s) 382, 508, 562, 586, 1027
BstMWI GCNNNNNNNGC 4 cut(s) 172, 209, 323, 857
BstNI CCWGG 3 cut(s) 740, 774, 845
BstSCI CCNGG 3 cut(s) 738, 772, 843
BstSFI CTRYAG 1 cut(s) 723
BstV1I GCAGC 7 cut(s) 39, 187, 190, 201, 709, 880, 1099
Bsu36I CCTNAGG 2 cut(s) 426, 612
BsuRI GGCC 2 cut(s) 102, 843
BtgZI GCGATG 1 cut(s) 719
BtsCI GGATG 4 cut(s) 176, 255, 569, 878
Cac8I GCNNGC 3 cut(s) 42, 328, 853
CfoI GCGC 3 cut(s) 214, 338, 687
Cfr13I GGNCC 1 cut(s) 903
CseI GACGC 1 cut(s) 1082
Csp6I GTAC 1 cut(s) 743
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 4 cut(s) 361, 599, 971, 1129
CviQI GTAC 1 cut(s) 743
DdeI CTNAG 3 cut(s) 369, 426, 612
DinI GGCGCC 1 cut(s) 337
DpnI GATC 5 cut(s) 384, 510, 564, 588, 1029
DpnII GATC 5 cut(s) 382, 508, 562, 586, 1027
DraI TTTAAA 1 cut(s) 768
DriI GACNNNNNGTC 1 cut(s) 364
Eam1104I CTCTTC 3 cut(s) 159, 660, 665
Eam1105I GACNNNNNGTC 1 cut(s) 364
EarI CTCTTC 3 cut(s) 159, 660, 665
Eco130I CCWWGG 2 cut(s) 279, 331
Eco47I GGWCC 1 cut(s) 903
Eco57I CTGAAG 2 cut(s) 684, 917
Eco72I CACGTG 1 cut(s) 1049
Eco81I CCTNAGG 2 cut(s) 426, 612
EcoRII CCWGG 3 cut(s) 738, 772, 843
EcoT14I CCWWGG 2 cut(s) 279, 331
EgeI GGCGCC 1 cut(s) 337
EheI GGCGCC 1 cut(s) 337
ErhI CCWWGG 2 cut(s) 279, 331
FaeI CATG 4 cut(s) 364, 602, 974, 1132
FaqI GGGAC 4 cut(s) 71, 96, 154, 932
FatI CATG 4 cut(s) 360, 598, 970, 1128
FauNDI CATATG 1 cut(s) 1138
FblI GTMKAC 1 cut(s) 275
Fnu4HI GCNGC 7 cut(s) 28, 201, 204, 215, 723, 894, 1113
FokI GGATG 4 cut(s) 163, 262, 556, 885
Fsp4HI GCNGC 7 cut(s) 28, 201, 204, 215, 723, 894, 1113
FspBI CTAG 4 cut(s) 147, 327, 525, 552
GlaI GCGC 3 cut(s) 213, 337, 686
GluI GCNGC 7 cut(s) 28, 201, 204, 215, 723, 894, 1113
GsaI CCCAGC 1 cut(s) 175
HaeII RGCGCY 2 cut(s) 339, 688
HaeIII GGCC 2 cut(s) 102, 843
HapII CCGG 2 cut(s) 45, 218
HgaI GACGC 1 cut(s) 1082
HhaI GCGC 3 cut(s) 214, 338, 687
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 4 cut(s) 364, 602, 974, 1132
Hin6I GCGC 3 cut(s) 212, 336, 685
HinP1I GCGC 3 cut(s) 212, 336, 685
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HinfI GANTC 6 cut(s) 7, 106, 365, 375, 1076, 1133
HpaII CCGG 2 cut(s) 45, 218
Hpy166II GTNNAC 1 cut(s) 276
Hpy188I TCNGA 5 cut(s) 249, 372, 664, 758, 835
Hpy188III TCNNGA 3 cut(s) 405, 611, 1080
Hpy8I GTNNAC 1 cut(s) 276
Hpy99I CGWCG 2 cut(s) 383, 720
HpyAV CCTTC 2 cut(s) 338, 1153
HpyCH4III ACNGT 2 cut(s) 817, 1006
HpyCH4IV ACGT 1 cut(s) 1048
HpyCH4V TGCA 4 cut(s) 725, 860, 998, 1115
HpyF10VI GCNNNNNNNGC 4 cut(s) 172, 209, 323, 857
HpyF3I CTNAG 3 cut(s) 369, 426, 612
HpySE526I ACGT 1 cut(s) 1048
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 4 cut(s) 364, 602, 974, 1132
HspAI GCGC 3 cut(s) 212, 336, 685
KasI GGCGCC 1 cut(s) 335
Kzo9I GATC 5 cut(s) 382, 508, 562, 586, 1027
LmnI GCTCC 2 cut(s) 541, 682
Lsp1109I GCAGC 7 cut(s) 39, 187, 190, 201, 709, 880, 1099
LweI GCATC 1 cut(s) 863
MaeI CTAG 4 cut(s) 147, 327, 525, 552
MaeII ACGT 1 cut(s) 1048
MaeIII GTNAC 1 cut(s) 8
MalI GATC 5 cut(s) 384, 510, 564, 588, 1029
MboI GATC 5 cut(s) 382, 508, 562, 586, 1027
MboII GAAGA 6 cut(s) 146, 377, 472, 475, 677, 682
MluCI AATT 9 cut(s) 133, 295, 431, 621, 706, 883, 953, 1083, 1174
Mly113I GGCGCC 1 cut(s) 336
MlyI GAGTC 4 cut(s) 16, 374, 384, 1085
MmeI TCCRAC 1 cut(s) 660
MseI TTAA 2 cut(s) 767, 827
MslI CAYNNNNRTG 1 cut(s) 975
MspI CCGG 2 cut(s) 45, 218
MspR9I CCNGG 3 cut(s) 740, 774, 845
Mva1269I GAATGC 2 cut(s) 860, 1010
MvaI CCWGG 3 cut(s) 740, 774, 845
MwoI GCNNNNNNNGC 4 cut(s) 172, 209, 323, 857
NarI GGCGCC 1 cut(s) 336
NdeI CATATG 1 cut(s) 1138
NdeII GATC 5 cut(s) 382, 508, 562, 586, 1027
NheI GCTAGC 1 cut(s) 326
NlaIII CATG 4 cut(s) 364, 602, 974, 1132
NlaIV GGNNCC 2 cut(s) 337, 905
NmuCI GTSAC 1 cut(s) 8
PctI GAATGC 2 cut(s) 860, 1010
PfeI GAWTC 2 cut(s) 106, 1133
PkrI GCNGC 7 cut(s) 29, 202, 205, 216, 724, 895, 1114
PleI GAGTC 4 cut(s) 15, 373, 383, 1084
PluTI GGCGCC 1 cut(s) 339
PmaCI CACGTG 1 cut(s) 1049
PmlI CACGTG 1 cut(s) 1049
PpsI GAGTC 4 cut(s) 15, 373, 383, 1084
Ppu21I YACGTR 1 cut(s) 1049
Psp6I CCWGG 3 cut(s) 738, 772, 843
PspCI CACGTG 1 cut(s) 1049
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 3 cut(s) 738, 772, 843
PspN4I GGNNCC 2 cut(s) 337, 905
PspPI GGNCC 1 cut(s) 903
PstI CTGCAG 1 cut(s) 727
RsaI GTAC 1 cut(s) 744
RsaNI GTAC 1 cut(s) 743
RseI CAYNNNNRTG 1 cut(s) 975
SalI GTCGAC 1 cut(s) 274
SaqAI TTAA 2 cut(s) 767, 827
SatI GCNGC 7 cut(s) 28, 201, 204, 215, 723, 894, 1113
Sau3AI GATC 5 cut(s) 382, 508, 562, 586, 1027
Sau96I GGNCC 1 cut(s) 903
SchI GAGTC 4 cut(s) 16, 374, 384, 1085
ScrFI CCNGG 3 cut(s) 740, 774, 845
SfaNI GCATC 1 cut(s) 863
SfcI CTRYAG 1 cut(s) 723
SfoI GGCGCC 1 cut(s) 337
SinI GGWCC 1 cut(s) 903
SmiMI CAYNNNNRTG 1 cut(s) 975
SmlI CTYRAG 2 cut(s) 21, 413
SmoI CTYRAG 2 cut(s) 21, 413
Sse9I AATT 9 cut(s) 133, 295, 431, 621, 706, 883, 953, 1083, 1174
SsiI CCGC 2 cut(s) 317, 324
SspDI GGCGCC 1 cut(s) 335
SspMI CTAG 4 cut(s) 147, 327, 525, 552
StyD4I CCNGG 3 cut(s) 738, 772, 843
StyI CCWWGG 2 cut(s) 279, 331
TaaI ACNGT 2 cut(s) 817, 1006
TaiI ACGT 1 cut(s) 1051
TaqI TCGA 4 cut(s) 275, 381, 406, 718
TasI AATT 9 cut(s) 133, 295, 431, 621, 706, 883, 953, 1083, 1174
TfiI GAWTC 2 cut(s) 106, 1133
Tru1I TTAA 2 cut(s) 767, 827
Tru9I TTAA 2 cut(s) 767, 827
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 7 cut(s) 27, 200, 203, 214, 722, 893, 1112
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 3 cut(s) 357, 722, 965
TspGWI ACGGA 2 cut(s) 760, 809
VpaK11BI GGWCC 1 cut(s) 903
XapI RAATTY 3 cut(s) 133, 431, 621
XmiI GTMKAC 1 cut(s) 275
XspI CTAG 4 cut(s) 147, 327, 525, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.