Rmu_sc0007150.1_g000008

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007150.1
OG0006404
Physical Location & Seq
Reverse (-)
37381 .. 38389
1009 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
atggcaacagctgcgctgctcattgaagccaacccattaatgaaattgaagcaaaacaccctattacgcacttttcgtcctcttccatcaaaaccaatcgcattaacgtttagcaatcaacgttgctttgctcctagaatatttaagaaccctaaacctcgatgtttcttgcccgccgactctaaatgtcatccctctcaatctcagtccctcaatgttcttaaagataatcggccagcggcagcaaaccctaatttcacagtcgccgttaataaggtatgggaaaagtgtcacagggcattgagggaaccagcaatggctgccgtgctgtttggtttgttagtcatgtgcaaccccaactcagcagcgttggctgctccggtgggtcgggtcggtggaagcttgacactcacgcgtacgacgttgcaccatccatctaaatattcttcgaccacaacggccgaccactacgatgatccgatctgggaaattgcattccattttttctttattttatactgtctctggggattgtttgcgttgctttcgccttaccccaagttttacacggctgctttgtttattttcgggacagttttcaccgctatttgggttcactctctaggtcaaacaagtgttgtcaagctccagcttcaggttactttatcgaacacaggacgagcagtgcaaacggatcttaatcggatcgctgaaactgcagatacctctacacgtacgggtttgagatacagcggtagctctatttcggcaacctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

258

Amino Acids

28.46

Weight (kDa)

9.87

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 216 - 250 1.3e-06 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 415
AciI CCGC 4 cut(s) 174, 239, 603, 753
AclI AACGTT 2 cut(s) 107, 121
AclWI GGATC 3 cut(s) 470, 702, 713
AcoI YGGCCR 2 cut(s) 233, 459
AcuI CTGAAG 1 cut(s) 638
AfaI GTAC 2 cut(s) 418, 736
AfiI CCNNNNNNNGG 2 cut(s) 609, 655
AflIII ACRYGT 2 cut(s) 413, 731
AgsI TTSAA 2 cut(s) 26, 49
AluBI AGCT 5 cut(s) 11, 402, 646, 652, 759
AluI AGCT 5 cut(s) 11, 402, 646, 652, 759
Alw26I GTCTC 1 cut(s) 527
AlwI GGATC 3 cut(s) 470, 702, 713
AoxI GGCC 2 cut(s) 233, 459
ApeKI GCWGC 7 cut(s) 11, 16, 242, 320, 365, 374, 572
AseI ATTAAT 1 cut(s) 38
AspLEI GCGC 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 592
BaeI ACNNNNGTAYC 2 cut(s) 739, 772
BbvI GCAGC 6 cut(s) 3, 254, 307, 361, 377, 559
BccI CCATC 3 cut(s) 94, 438, 442
BceAI ACGGC 4 cut(s) 251, 308, 474, 585
BcoDI GTCTC 1 cut(s) 527
BfaI CTAG 2 cut(s) 135, 623
BfmI CTRYAG 1 cut(s) 717
BisI GCNGC 8 cut(s) 12, 17, 240, 243, 321, 366, 375, 573
BlsI GCNGC 8 cut(s) 13, 18, 241, 244, 322, 367, 376, 574
BmiI GGNNCC 1 cut(s) 309
BpmI CTGGAG 1 cut(s) 632
BsaAI YACGTR 1 cut(s) 734
BsaBI GATNNNNATC 1 cut(s) 699
BsaWI WCCGGW 1 cut(s) 379
Bsc4I CCNNNNNNNGG 2 cut(s) 609, 655
Bse3DI GCAATG 1 cut(s) 321
Bse8I GATNNNNATC 1 cut(s) 699
BseGI GGATG 2 cut(s) 190, 430
BseJI GATNNNNATC 1 cut(s) 699
BseLI CCNNNNNNNGG 2 cut(s) 609, 655
BseMI GCAATG 1 cut(s) 321
BseMII CTCAG 2 cut(s) 218, 375
BseX3I CGGCCG 1 cut(s) 459
BseXI GCAGC 6 cut(s) 3, 254, 307, 361, 377, 559
Bsh1236I CGCG 1 cut(s) 415
Bsh1285I CGRYCG 1 cut(s) 462
BshFI GGCC 2 cut(s) 235, 461
BsiEI CGRYCG 1 cut(s) 462
BsiSI CCGG 1 cut(s) 380
BsiWI CGTACG 2 cut(s) 416, 734
BslFI GGGAC 2 cut(s) 193, 604
BslI CCNNNNNNNGG 2 cut(s) 609, 655
BsmAI GTCTC 1 cut(s) 527
BsmFI GGGAC 2 cut(s) 193, 604
BsmI GAATGC 1 cut(s) 494
BsnI GGCC 2 cut(s) 235, 461
Bsp143I GATC 4 cut(s) 475, 480, 694, 705
BspACI CCGC 4 cut(s) 174, 239, 603, 753
BspANI GGCC 2 cut(s) 235, 461
BspCNI CTCAG 2 cut(s) 217, 374
BspFNI CGCG 1 cut(s) 415
BspLI GGNNCC 1 cut(s) 309
BspMAI CTGCAG 1 cut(s) 721
BspPI GGATC 3 cut(s) 470, 702, 713
BsrDI GCAATG 1 cut(s) 321
BssMI GATC 4 cut(s) 475, 480, 694, 705
Bst4CI ACNGT 3 cut(s) 262, 521, 595
Bst6I CTCTTC 1 cut(s) 87
BstAPI GCANNNNNTGC 2 cut(s) 11, 320
BstBAI YACGTR 1 cut(s) 734
BstC8I GCNNGC 2 cut(s) 174, 237
BstDEI CTNAG 2 cut(s) 204, 361
BstF5I GGATG 2 cut(s) 190, 430
BstFNI CGCG 1 cut(s) 415
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 4 cut(s) 478, 483, 697, 708
BstMAI GTCTC 1 cut(s) 527
BstMBI GATC 4 cut(s) 475, 480, 694, 705
BstMCI CGRYCG 1 cut(s) 462
BstMWI GCNNNNNNNGC 5 cut(s) 11, 320, 371, 374, 716
BstSFI CTRYAG 1 cut(s) 717
BstUI CGCG 1 cut(s) 415
BstV1I GCAGC 6 cut(s) 3, 254, 307, 361, 377, 559
BstX2I RGATCY 1 cut(s) 694
BstYI RGATCY 1 cut(s) 694
BstZI CGGCCG 1 cut(s) 459
BsuRI GGCC 2 cut(s) 235, 461
BtsCI GGATG 2 cut(s) 190, 430
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 1 cut(s) 690
Cac8I GCNNGC 2 cut(s) 174, 237
CfoI GCGC 1 cut(s) 16
Csp6I GTAC 2 cut(s) 417, 735
CviAII CATG 1 cut(s) 346
CviQI GTAC 2 cut(s) 417, 735
DdeI CTNAG 2 cut(s) 204, 361
DpnI GATC 4 cut(s) 477, 482, 696, 707
DpnII GATC 4 cut(s) 475, 480, 694, 705
EaeI YGGCCR 2 cut(s) 233, 459
EagI CGGCCG 1 cut(s) 459
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
EclXI CGGCCG 1 cut(s) 459
Eco52I CGGCCG 1 cut(s) 459
Eco57I CTGAAG 1 cut(s) 638
FaeI CATG 1 cut(s) 349
FaiI YATR 3 cut(s) 280, 347, 517
FaqI GGGAC 2 cut(s) 193, 604
FatI CATG 1 cut(s) 345
FauI CCCGC 1 cut(s) 181
Fnu4HI GCNGC 8 cut(s) 12, 17, 240, 243, 321, 366, 375, 573
FokI GGATG 2 cut(s) 177, 417
Fsp4HI GCNGC 8 cut(s) 12, 17, 240, 243, 321, 366, 375, 573
FspBI CTAG 2 cut(s) 135, 623
GlaI GCGC 1 cut(s) 15
GluI GCNGC 8 cut(s) 12, 17, 240, 243, 321, 366, 375, 573
GsuI CTGGAG 1 cut(s) 632
HaeIII GGCC 2 cut(s) 235, 461
HapII CCGG 1 cut(s) 380
HhaI GCGC 1 cut(s) 16
Hin1II CATG 1 cut(s) 349
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 1 cut(s) 179
HpaII CCGG 1 cut(s) 380
HphI GGTGA 1 cut(s) 592
Hpy166II GTNNAC 1 cut(s) 616
Hpy188I TCNGA 2 cut(s) 480, 705
Hpy188III TCNNGA 1 cut(s) 589
Hpy8I GTNNAC 1 cut(s) 616
Hpy99I CGWCG 1 cut(s) 424
HpyCH4III ACNGT 3 cut(s) 262, 521, 595
HpyCH4IV ACGT 4 cut(s) 107, 121, 422, 733
HpyCH4V TGCA 5 cut(s) 351, 427, 494, 688, 719
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 320, 371, 374, 716
HpyF3I CTNAG 2 cut(s) 204, 361
HpySE526I ACGT 4 cut(s) 107, 121, 422, 733
Hsp92II CATG 1 cut(s) 349
HspAI GCGC 1 cut(s) 14
Kzo9I GATC 4 cut(s) 475, 480, 694, 705
LmnI GCTCC 3 cut(s) 136, 382, 651
LpnPI CCDG 9 cut(s) 249, 280, 324, 393, 469, 511, 641, 660, 662
Lsp1109I GCAGC 6 cut(s) 3, 254, 307, 361, 377, 559
MaeI CTAG 2 cut(s) 135, 623
MaeII ACGT 4 cut(s) 107, 121, 422, 733
MaeIII GTNAC 2 cut(s) 290, 658
MalI GATC 4 cut(s) 477, 482, 696, 707
MboI GATC 4 cut(s) 475, 480, 694, 705
MboII GAAGA 2 cut(s) 74, 438
MflI RGATCY 1 cut(s) 694
MluCI AATT 3 cut(s) 44, 253, 489
MluI ACGCGT 1 cut(s) 413
MlyI GAGTC 1 cut(s) 173
MnlI CCTC 6 cut(s) 90, 168, 205, 221, 297, 736
MseI TTAA 6 cut(s) 38, 104, 144, 222, 270, 699
MslI CAYNNNNRTG 1 cut(s) 471
MspA1I CMGCKG 3 cut(s) 11, 239, 753
MspI CCGG 1 cut(s) 380
Mva1269I GAATGC 1 cut(s) 494
MvnI CGCG 1 cut(s) 415
MwoI GCNNNNNNNGC 5 cut(s) 11, 320, 371, 374, 716
NdeII GATC 4 cut(s) 475, 480, 694, 705
NlaIII CATG 1 cut(s) 349
NlaIV GGNNCC 1 cut(s) 309
NmuCI GTSAC 1 cut(s) 290
PcsI WCGNNNNNNNCGW 2 cut(s) 73, 419
PctI GAATGC 1 cut(s) 494
Pfl23II CGTACG 2 cut(s) 416, 734
PkrI GCNGC 8 cut(s) 13, 18, 241, 244, 322, 367, 376, 574
PleI GAGTC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 173
Ppu21I YACGTR 1 cut(s) 734
PshBI ATTAAT 1 cut(s) 38
Psp1406I AACGTT 2 cut(s) 107, 121
PspLI CGTACG 2 cut(s) 416, 734
PspN4I GGNNCC 1 cut(s) 309
PstI CTGCAG 1 cut(s) 721
PsuI RGATCY 1 cut(s) 694
PvuII CAGCTG 1 cut(s) 11
RsaI GTAC 2 cut(s) 418, 736
RsaNI GTAC 2 cut(s) 417, 735
RseI CAYNNNNRTG 1 cut(s) 471
SaqAI TTAA 6 cut(s) 38, 104, 144, 222, 270, 699
SatI GCNGC 8 cut(s) 12, 17, 240, 243, 321, 366, 375, 573
Sau3AI GATC 4 cut(s) 475, 480, 694, 705
SchI GAGTC 1 cut(s) 173
SfcI CTRYAG 1 cut(s) 717
SmiMI CAYNNNNRTG 1 cut(s) 471
Sse9I AATT 3 cut(s) 44, 253, 489
SsiI CCGC 4 cut(s) 174, 239, 603, 753
SspI AATATT 2 cut(s) 141, 443
SspMI CTAG 2 cut(s) 135, 623
TaaI ACNGT 3 cut(s) 262, 521, 595
TaiI ACGT 4 cut(s) 110, 124, 425, 736
TaqI TCGA 3 cut(s) 160, 449, 668
TasI AATT 3 cut(s) 44, 253, 489
TauI GCSGC 1 cut(s) 242
Tru1I TTAA 6 cut(s) 38, 104, 144, 222, 270, 699
Tru9I TTAA 6 cut(s) 38, 104, 144, 222, 270, 699
TscAI CASTG 1 cut(s) 690
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 7 cut(s) 11, 16, 242, 320, 365, 374, 572
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 1 cut(s) 56
TspGWI ACGGA 1 cut(s) 707
TspRI CASTG 1 cut(s) 690
VspI ATTAAT 1 cut(s) 38
XspI CTAG 2 cut(s) 135, 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.