Rh2AG652200

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0006404
Physical Location & Seq
Reverse (-)
87014890 .. 87016309
1420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG652200.1

Sequence Viewer

Length: 717 bp
ATGGCTGCCGTGCTGTTGGGTTTGTTAGTCATGTACAATCCCAACTCAGCAGCGTTGGCTGCTCCTGTAGGTCGGGTCGGTAGAAGCTTGACACCCATCCGTACGACGTTGCACAATCCATCTAAATGTTTGACCACAATCACAACGTCCTACGATGATCCGATCTGGAGTTTGCGGCCTTATCGTCAAACAAGTGTTGTCAAGCTCCAGCTTCAGGTTACTTCATCGAACACGGGACGAGCAGTGCAAACGGATCTTAATCGGATCGCTGAAACTGCAGATACCTCTACACCTACGGGTTTGAGATGTGTTTTGAGTGATACAGCACCAGCTCTATCTCGGCAACCTGATTATTGCATTTCCGGTTACTCATGTGTGGTTATACCAAAGTTCTCTAGAGCAGCGGCACAGCAATGCTTTTATCAACTTGTGTTCAGAGAGACGGTTAGATATGATGAAGAGACCATTGTCAATGTGGACAATATGAAAACTCAAAGCAGAACAAGTCAGAGATCAACTAATGAATCTCACAATGACAATGTAGAGGTTACAATTTTACTTGCTGTTGAAGGAAAGTCTAAGTTGCCCACTATCATTGACAGCAGTGACTTGAAGAAAGTGATGGAAGAGCTTGCATCCATTGATCCCAGCAGACTAATATTTGGATTAGAGGTACGGTGGACTCCTCAGTTCTCTTCAAATGCGGCAAGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

238

Amino Acids

26.2

Weight (kDa)

8.6

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 64 - 233 6.1e-39 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 175, 404, 704
AclWI GGATC 4 cut(s) 152, 261, 272, 638
AcuI CTGAAG 1 cut(s) 197
AfaI GTAC 3 cut(s) 35, 103, 675
AfiI CCNNNNNNNGG 1 cut(s) 214
AgsI TTSAA 3 cut(s) 569, 613, 699
AluBI AGCT 5 cut(s) 87, 205, 211, 332, 631
AluI AGCT 5 cut(s) 87, 205, 211, 332, 631
Alw26I GTCTC 2 cut(s) 434, 455
AlwI GGATC 4 cut(s) 152, 261, 272, 638
AoxI GGCC 1 cut(s) 176
ApeKI GCWGC 4 cut(s) 5, 50, 59, 401
BbvI GCAGC 3 cut(s) 46, 62, 413
BccI CCATC 3 cut(s) 104, 127, 616
BcoDI GTCTC 2 cut(s) 434, 455
BfaI CTAG 1 cut(s) 396
BfmI CTRYAG 2 cut(s) 66, 276
BisI GCNGC 7 cut(s) 6, 51, 60, 176, 402, 405, 705
BlsI GCNGC 7 cut(s) 7, 52, 61, 177, 403, 406, 706
BmsI GCATC 1 cut(s) 644
BoxI GACNNNNGTC 1 cut(s) 467
BpmI CTGGAG 2 cut(s) 187, 191
BsaBI GATNNNNATC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 455
BsaWI WCCGGW 1 cut(s) 362
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 419
Bse8I GATNNNNATC 1 cut(s) 258
BseGI GGATG 2 cut(s) 96, 635
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMI GCAATG 1 cut(s) 419
BseMII CTCAG 2 cut(s) 60, 701
BseRI GAGGAG 1 cut(s) 675
BseXI GCAGC 3 cut(s) 46, 62, 413
BseYI CCCAGC 1 cut(s) 647
BshFI GGCC 1 cut(s) 178
BsiSI CCGG 1 cut(s) 363
BsiWI CGTACG 1 cut(s) 101
BslFI GGGAC 1 cut(s) 249
BslI CCNNNNNNNGG 1 cut(s) 214
BsmAI GTCTC 2 cut(s) 434, 455
BsmBI CGTCTC 1 cut(s) 434
BsmFI GGGAC 1 cut(s) 249
BsnI GGCC 1 cut(s) 178
Bso31I GGTCTC 1 cut(s) 455
Bsp1407I TGTACA 1 cut(s) 33
Bsp143I GATC 6 cut(s) 157, 162, 253, 264, 512, 643
BspACI CCGC 3 cut(s) 175, 404, 704
BspANI GGCC 1 cut(s) 178
BspCNI CTCAG 2 cut(s) 59, 700
BspMAI CTGCAG 1 cut(s) 280
BspPI GGATC 4 cut(s) 152, 261, 272, 638
BspQI GCTCTTC 1 cut(s) 621
BspTNI GGTCTC 1 cut(s) 455
BsrDI GCAATG 1 cut(s) 419
BsrGI TGTACA 1 cut(s) 33
BssMI GATC 6 cut(s) 157, 162, 253, 264, 512, 643
Bst4CI ACNGT 2 cut(s) 445, 678
Bst6I CTCTTC 3 cut(s) 453, 621, 700
BstAUI TGTACA 1 cut(s) 33
BstC8I GCNNGC 2 cut(s) 633, 709
BstDEI CTNAG 3 cut(s) 46, 579, 687
BstF5I GGATG 2 cut(s) 96, 635
BstKTI GATC 6 cut(s) 160, 165, 256, 267, 515, 646
BstMAI GTCTC 2 cut(s) 434, 455
BstMBI GATC 6 cut(s) 157, 162, 253, 264, 512, 643
BstMWI GCNNNNNNNGC 3 cut(s) 56, 59, 275
BstPAI GACNNNNGTC 1 cut(s) 467
BstSFI CTRYAG 2 cut(s) 66, 276
BstV1I GCAGC 3 cut(s) 46, 62, 413
BstX2I RGATCY 1 cut(s) 253
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 1 cut(s) 178
BtsCI GGATG 2 cut(s) 96, 635
BtsI GCAGTG 2 cut(s) 249, 610
BtsIMutI CAGTG 2 cut(s) 249, 610
Cac8I GCNNGC 2 cut(s) 633, 709
Csp6I GTAC 3 cut(s) 34, 102, 674
CspCI CAANNNNNGTGG 2 cut(s) 577, 612
CviAII CATG 2 cut(s) 31, 372
CviJI RGCY 8 cut(s) 5, 59, 87, 178, 205, 211, 332, 631
CviKI_1 RGCY 8 cut(s) 5, 59, 87, 178, 205, 211, 332, 631
CviQI GTAC 3 cut(s) 34, 102, 674
DdeI CTNAG 3 cut(s) 46, 579, 687
DpnI GATC 6 cut(s) 159, 164, 255, 266, 514, 645
DpnII GATC 6 cut(s) 157, 162, 253, 264, 512, 643
Eam1104I CTCTTC 3 cut(s) 453, 621, 700
EarI CTCTTC 3 cut(s) 453, 621, 700
Eco31I GGTCTC 1 cut(s) 455
Eco57I CTGAAG 1 cut(s) 197
Esp3I CGTCTC 1 cut(s) 434
FaeI CATG 2 cut(s) 34, 375
FaiI YATR 5 cut(s) 32, 373, 383, 453, 485
FaqI GGGAC 1 cut(s) 249
FatI CATG 2 cut(s) 30, 371
Fnu4HI GCNGC 7 cut(s) 6, 51, 60, 176, 402, 405, 705
FokI GGATG 2 cut(s) 83, 622
Fsp4HI GCNGC 7 cut(s) 6, 51, 60, 176, 402, 405, 705
FspBI CTAG 1 cut(s) 396
GluI GCNGC 7 cut(s) 6, 51, 60, 176, 402, 405, 705
GsaI CCCAGC 1 cut(s) 651
GsuI CTGGAG 2 cut(s) 187, 191
HaeIII GGCC 1 cut(s) 178
HapII CCGG 1 cut(s) 363
Hin1II CATG 2 cut(s) 34, 375
HindIII AAGCTT 1 cut(s) 85
HinfI GANTC 2 cut(s) 524, 682
HpaII CCGG 1 cut(s) 363
Hpy166II GTNNAC 2 cut(s) 478, 681
Hpy188I TCNGA 4 cut(s) 162, 264, 437, 510
Hpy188III TCNNGA 2 cut(s) 166, 396
Hpy8I GTNNAC 2 cut(s) 478, 681
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 1 cut(s) 563
HpyCH4III ACNGT 2 cut(s) 445, 678
HpyCH4IV ACGT 2 cut(s) 107, 146
HpyCH4V TGCA 5 cut(s) 112, 247, 278, 357, 635
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 59, 275
HpyF3I CTNAG 3 cut(s) 46, 579, 687
HpySE526I ACGT 2 cut(s) 107, 146
Hsp92II CATG 2 cut(s) 34, 375
Kzo9I GATC 6 cut(s) 157, 162, 253, 264, 512, 643
LguI GCTCTTC 1 cut(s) 621
LmnI GCTCC 2 cut(s) 67, 210
LpnPI CCDG 8 cut(s) 78, 151, 200, 221, 342, 360, 376, 661
Lsp1109I GCAGC 3 cut(s) 46, 62, 413
LweI GCATC 1 cut(s) 644
MaeI CTAG 1 cut(s) 396
MaeII ACGT 2 cut(s) 107, 146
MaeIII GTNAC 4 cut(s) 217, 365, 547, 605
MalI GATC 6 cut(s) 159, 164, 255, 266, 514, 645
MboI GATC 6 cut(s) 157, 162, 253, 264, 512, 643
MboII GAAGA 4 cut(s) 470, 625, 638, 687
MflI RGATCY 1 cut(s) 253
MluCI AATT 1 cut(s) 552
MlyI GAGTC 1 cut(s) 676
MnlI CCTC 4 cut(s) 295, 538, 664, 696
MseI TTAA 1 cut(s) 258
MslI CAYNNNNRTG 2 cut(s) 124, 412
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 1 cut(s) 363
MwoI GCNNNNNNNGC 3 cut(s) 56, 59, 275
NdeII GATC 6 cut(s) 157, 162, 253, 264, 512, 643
NlaIII CATG 2 cut(s) 34, 375
NmeAIII GCCGAG 1 cut(s) 319
NmuCI GTSAC 1 cut(s) 605
PciSI GCTCTTC 1 cut(s) 621
PfeI GAWTC 1 cut(s) 524
Pfl23II CGTACG 1 cut(s) 101
PkrI GCNGC 7 cut(s) 7, 52, 61, 177, 403, 406, 706
PleI GAGTC 1 cut(s) 676
PpsI GAGTC 1 cut(s) 676
PshAI GACNNNNGTC 1 cut(s) 467
PspFI CCCAGC 1 cut(s) 647
PspLI CGTACG 1 cut(s) 101
PstI CTGCAG 1 cut(s) 280
PsuI RGATCY 1 cut(s) 253
RsaI GTAC 3 cut(s) 35, 103, 675
RsaNI GTAC 3 cut(s) 34, 102, 674
RseI CAYNNNNRTG 2 cut(s) 124, 412
SapI GCTCTTC 1 cut(s) 621
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 7 cut(s) 6, 51, 60, 176, 402, 405, 705
Sau3AI GATC 6 cut(s) 157, 162, 253, 264, 512, 643
SchI GAGTC 1 cut(s) 676
SfaNI GCATC 1 cut(s) 644
SfcI CTRYAG 2 cut(s) 66, 276
SmiMI CAYNNNNRTG 2 cut(s) 124, 412
Sse9I AATT 1 cut(s) 552
SsiI CCGC 3 cut(s) 175, 404, 704
SspI AATATT 1 cut(s) 660
SspMI CTAG 1 cut(s) 396
TaaI ACNGT 2 cut(s) 445, 678
TaiI ACGT 2 cut(s) 110, 149
TaqI TCGA 1 cut(s) 227
TasI AATT 1 cut(s) 552
TatI WGTACW 1 cut(s) 33
TauI GCSGC 3 cut(s) 178, 407, 707
TfiI GAWTC 1 cut(s) 524
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 2 cut(s) 249, 610
TseFI GTSAC 1 cut(s) 605
TseI GCWGC 4 cut(s) 5, 50, 59, 401
Tsp45I GTSAC 1 cut(s) 605
TspDTI ATGAA 4 cut(s) 213, 471, 500, 537
TspGWI ACGGA 2 cut(s) 89, 266
TspRI CASTG 2 cut(s) 249, 610
XbaI TCTAGA 1 cut(s) 395
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XspI CTAG 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.