Rh2BG663000

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0006404
Physical Location & Seq
Reverse (-)
87707699 .. 87709118
1420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG663000.1

Sequence Viewer

Length: 603 bp
ATGGCTGCCGTGCTGTTGGGTTTGTTAGTCATGTACAATCCCAACTCAGCAGCGTTGGCTGCTCCTGTAGGTCGGGTCGGTAGAAGCTTGACACCCATCCGTACGACGTTGCACAATCCATCTAAATGTTTGACCACAATCACAACGTCCTACGATGATCCGATCTGGAGTTTGCGGCCTTATCGTCAAACAAGTGTTGTCAAGCTCCAGCTTCAGATACAGCACCAGCTCTATCTCGGCAACCTGATTATTGCATTTCCGGTGGTTATACCAAAGTTCTCTAGAGCAGCGGCACAGCAATGCTTTTATCAACTTGTGTTCAGAGAGACGGTTAGATATGATGAAGAGACCATTGTCAATGTGGACAATATGAAAACTCAAAGCAGAACAAGTCAGAGATCAACTAATGAATCTCACAATGACAATGTAGAGGTTACAATTTTACTTGCTGTTGAAGGAAAGTCTAAGTTGCCCACTATCATTGACAGCAGTGACTTGAAGAAAGTGATGGAAGAGCTTGCATCCATTGATCCCAGCAGACTAATATTTGGATTAGAGGTACGGTGGACTCCTCAGTTCTCTTCAAATGCGGCAAGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

200

Amino Acids

22.38

Weight (kDa)

9.14

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 93 - 195 6.4e-20 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 175, 290, 590
AclWI GGATC 2 cut(s) 152, 524
AcuI CTGAAG 1 cut(s) 197
AfaI GTAC 3 cut(s) 35, 103, 561
AgsI TTSAA 3 cut(s) 455, 499, 585
AluBI AGCT 5 cut(s) 87, 205, 211, 229, 517
AluI AGCT 5 cut(s) 87, 205, 211, 229, 517
Alw26I GTCTC 2 cut(s) 320, 341
AlwI GGATC 2 cut(s) 152, 524
AoxI GGCC 1 cut(s) 176
ApeKI GCWGC 4 cut(s) 5, 50, 59, 287
BbvI GCAGC 3 cut(s) 46, 62, 299
BccI CCATC 3 cut(s) 104, 127, 502
BcoDI GTCTC 2 cut(s) 320, 341
BfaI CTAG 1 cut(s) 282
BfmI CTRYAG 1 cut(s) 66
BisI GCNGC 7 cut(s) 6, 51, 60, 176, 288, 291, 591
BlsI GCNGC 7 cut(s) 7, 52, 61, 177, 289, 292, 592
BmsI GCATC 1 cut(s) 530
BoxI GACNNNNGTC 1 cut(s) 353
BpmI CTGGAG 2 cut(s) 187, 191
BsaI GGTCTC 1 cut(s) 341
BsaWI WCCGGW 1 cut(s) 259
Bse3DI GCAATG 1 cut(s) 305
BseGI GGATG 2 cut(s) 96, 521
BseMI GCAATG 1 cut(s) 305
BseMII CTCAG 2 cut(s) 60, 587
BseRI GAGGAG 1 cut(s) 561
BseXI GCAGC 3 cut(s) 46, 62, 299
BseYI CCCAGC 1 cut(s) 533
BshFI GGCC 1 cut(s) 178
BsiSI CCGG 1 cut(s) 260
BsiWI CGTACG 1 cut(s) 101
BsmAI GTCTC 2 cut(s) 320, 341
BsmBI CGTCTC 1 cut(s) 320
BsnI GGCC 1 cut(s) 178
Bso31I GGTCTC 1 cut(s) 341
Bsp1407I TGTACA 1 cut(s) 33
Bsp143I GATC 4 cut(s) 157, 162, 398, 529
BspACI CCGC 3 cut(s) 175, 290, 590
BspANI GGCC 1 cut(s) 178
BspCNI CTCAG 2 cut(s) 59, 586
BspPI GGATC 2 cut(s) 152, 524
BspQI GCTCTTC 1 cut(s) 507
BspTNI GGTCTC 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 305
BsrGI TGTACA 1 cut(s) 33
BssMI GATC 4 cut(s) 157, 162, 398, 529
Bst4CI ACNGT 2 cut(s) 331, 564
Bst6I CTCTTC 3 cut(s) 339, 507, 586
BstAUI TGTACA 1 cut(s) 33
BstC8I GCNNGC 2 cut(s) 519, 595
BstDEI CTNAG 3 cut(s) 46, 465, 573
BstF5I GGATG 2 cut(s) 96, 521
BstKTI GATC 4 cut(s) 160, 165, 401, 532
BstMAI GTCTC 2 cut(s) 320, 341
BstMBI GATC 4 cut(s) 157, 162, 398, 529
BstMWI GCNNNNNNNGC 2 cut(s) 56, 59
BstPAI GACNNNNGTC 1 cut(s) 353
BstSFI CTRYAG 1 cut(s) 66
BstV1I GCAGC 3 cut(s) 46, 62, 299
BsuRI GGCC 1 cut(s) 178
BtsCI GGATG 2 cut(s) 96, 521
BtsI GCAGTG 1 cut(s) 496
BtsIMutI CAGTG 1 cut(s) 496
Cac8I GCNNGC 2 cut(s) 519, 595
Csp6I GTAC 3 cut(s) 34, 102, 560
CspCI CAANNNNNGTGG 2 cut(s) 463, 498
CviAII CATG 1 cut(s) 31
CviJI RGCY 8 cut(s) 5, 59, 87, 178, 205, 211, 229, 517
CviKI_1 RGCY 8 cut(s) 5, 59, 87, 178, 205, 211, 229, 517
CviQI GTAC 3 cut(s) 34, 102, 560
DdeI CTNAG 3 cut(s) 46, 465, 573
DpnI GATC 4 cut(s) 159, 164, 400, 531
DpnII GATC 4 cut(s) 157, 162, 398, 529
Eam1104I CTCTTC 3 cut(s) 339, 507, 586
EarI CTCTTC 3 cut(s) 339, 507, 586
Eco31I GGTCTC 1 cut(s) 341
Eco57I CTGAAG 1 cut(s) 197
Esp3I CGTCTC 1 cut(s) 320
FaeI CATG 1 cut(s) 34
FaiI YATR 4 cut(s) 32, 269, 339, 371
FatI CATG 1 cut(s) 30
Fnu4HI GCNGC 7 cut(s) 6, 51, 60, 176, 288, 291, 591
FokI GGATG 2 cut(s) 83, 508
Fsp4HI GCNGC 7 cut(s) 6, 51, 60, 176, 288, 291, 591
FspBI CTAG 1 cut(s) 282
GluI GCNGC 7 cut(s) 6, 51, 60, 176, 288, 291, 591
GsaI CCCAGC 1 cut(s) 537
GsuI CTGGAG 2 cut(s) 187, 191
HaeIII GGCC 1 cut(s) 178
HapII CCGG 1 cut(s) 260
Hin1II CATG 1 cut(s) 34
HindIII AAGCTT 1 cut(s) 85
HinfI GANTC 2 cut(s) 410, 568
HpaII CCGG 1 cut(s) 260
Hpy166II GTNNAC 2 cut(s) 364, 567
Hpy188I TCNGA 4 cut(s) 162, 216, 323, 396
Hpy188III TCNNGA 2 cut(s) 166, 282
Hpy8I GTNNAC 2 cut(s) 364, 567
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 1 cut(s) 449
HpyCH4III ACNGT 2 cut(s) 331, 564
HpyCH4IV ACGT 2 cut(s) 107, 146
HpyCH4V TGCA 3 cut(s) 112, 254, 521
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 59
HpyF3I CTNAG 3 cut(s) 46, 465, 573
HpySE526I ACGT 2 cut(s) 107, 146
Hsp92II CATG 1 cut(s) 34
Kzo9I GATC 4 cut(s) 157, 162, 398, 529
LguI GCTCTTC 1 cut(s) 507
LmnI GCTCC 2 cut(s) 67, 210
LpnPI CCDG 7 cut(s) 78, 151, 221, 239, 257, 273, 547
Lsp1109I GCAGC 3 cut(s) 46, 62, 299
LweI GCATC 1 cut(s) 530
MaeI CTAG 1 cut(s) 282
MaeII ACGT 2 cut(s) 107, 146
MaeIII GTNAC 2 cut(s) 433, 491
MalI GATC 4 cut(s) 159, 164, 400, 531
MboI GATC 4 cut(s) 157, 162, 398, 529
MboII GAAGA 4 cut(s) 356, 511, 524, 573
MluCI AATT 1 cut(s) 438
MlyI GAGTC 1 cut(s) 562
MnlI CCTC 3 cut(s) 424, 550, 582
MslI CAYNNNNRTG 2 cut(s) 124, 298
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 1 cut(s) 260
MwoI GCNNNNNNNGC 2 cut(s) 56, 59
NdeII GATC 4 cut(s) 157, 162, 398, 529
NlaIII CATG 1 cut(s) 34
NmeAIII GCCGAG 1 cut(s) 216
NmuCI GTSAC 1 cut(s) 491
PciSI GCTCTTC 1 cut(s) 507
PfeI GAWTC 1 cut(s) 410
Pfl23II CGTACG 1 cut(s) 101
PkrI GCNGC 7 cut(s) 7, 52, 61, 177, 289, 292, 592
PleI GAGTC 1 cut(s) 562
PpsI GAGTC 1 cut(s) 562
PshAI GACNNNNGTC 1 cut(s) 353
PspFI CCCAGC 1 cut(s) 533
PspLI CGTACG 1 cut(s) 101
RsaI GTAC 3 cut(s) 35, 103, 561
RsaNI GTAC 3 cut(s) 34, 102, 560
RseI CAYNNNNRTG 2 cut(s) 124, 298
SapI GCTCTTC 1 cut(s) 507
SatI GCNGC 7 cut(s) 6, 51, 60, 176, 288, 291, 591
Sau3AI GATC 4 cut(s) 157, 162, 398, 529
SchI GAGTC 1 cut(s) 562
SfaNI GCATC 1 cut(s) 530
SfcI CTRYAG 1 cut(s) 66
SmiMI CAYNNNNRTG 2 cut(s) 124, 298
Sse9I AATT 1 cut(s) 438
SsiI CCGC 3 cut(s) 175, 290, 590
SspI AATATT 1 cut(s) 546
SspMI CTAG 1 cut(s) 282
TaaI ACNGT 2 cut(s) 331, 564
TaiI ACGT 2 cut(s) 110, 149
TasI AATT 1 cut(s) 438
TatI WGTACW 1 cut(s) 33
TauI GCSGC 3 cut(s) 178, 293, 593
TfiI GAWTC 1 cut(s) 410
TscAI CASTG 1 cut(s) 496
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 4 cut(s) 5, 50, 59, 287
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 3 cut(s) 357, 386, 423
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 1 cut(s) 496
XbaI TCTAGA 1 cut(s) 281
XcmI CCANNNNNNNNNTGG 1 cut(s) 358
XspI CTAG 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.