Rroxscaffold_4G00327970

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006404
Physical Location & Seq
Reverse (-)
60842310 .. 60849849
7540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00327970.1

Sequence Viewer

Length: 1191 bp
ATGGCAACAGCTGCGCTGCTCATTGAAGCCAACCCATTAATGAAATTGAAGCAAAACACCCTATTACGCACTTTTCGTCCTCTTCCATCAAAACCAATCGCATTAACGTTTAGCAATCAACGTTGCTTTGCTCCTAGAATATTTAAGAACCCTAAACCTCAATGTTTCTTGCCCGCCGACTCTAAATGTCATCCCTCTCAGTCTCAGTCCCTCAATGTTCTTAAAGATAATCGGCCAGCAGCAGCAAACCCTAATTTCACAGGCGCCGTTAATAAGGTATGGGAAAAGTGTCACAAGGCATTGAGGGAACCAGCAATGGCTGCCGTGCTGTTTGGTTTGTTAGTCATGTGCAACCCCAACTCAGCAGCGTTGGCTGCTCCGGTGGGTCGGGTCGGTGGAAGCTTGACACCCACGCGTACGACGTTGCACCATCCATCTAAATATTCTTCGACCACAATCACAACGTCCGACCACTACGATGATCCGATCTGGGAAATTGCATTCTATTTTTTCTTTATTTTATACTTTCTCTGGGGATTGTTTGCGTTGCTTTCGCCTTACCCCAAGTTTTACACGGCTGCTTTGTTTATTTTCGGGACAGTTTTCACCGCTATTTGGGTTCACTCTCTAGGTCAAACAAGTGTTGTCAAGCTCCAGCTTCAGGTATATGAGAATAACACGGGACGAGCAGTGCAAACGGATCTTAATCGGATCGCTGAAACTGCAGATACCTCTACACGTACGGGTTTGAGGTATGTTTTGAGAGATACAGCTGTAGCTCTATTTCGGCAACCTGATTATTGCATTTCCGGTTACTCATGTGTGGGTGTACCAAAGTTCACTAGAGCGGCTGCACAGCAATGCTTTGATAAAATTGTGTTCAGAGAGAGGGTTAGATATGACGAAGAGACCATTGTCAATGTGGACAATATAAAAACTCAAAGCACAACAAGTCAGAGATCAACTACTGAATCTCACAATGACAATGTAGAGGTTACAATTTTACTTGCTGTTGATGGAAAGTCTAAGTTGCCCACTATCATTGACAGCAGTGATTTGAAGAAAGTGATGGAAGAGCTTGCATCCATTGATCCCAGCAGACTACTTTTAGTAAGAATAAAAATTTCCACTAAATTGGCAAGCAGCAAATCCAGGAAGTCTCAAGCTGTTCGCAGTGGCGGTTCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

396

Amino Acids

43.95

Weight (kDa)

9.58

Isoelectric Point (pI)

41.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 213 - 372 1.7e-36 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 263
AccBSI CCGCTC 1 cut(s) 848
AccII CGCG 1 cut(s) 415
AciI CCGC 4 cut(s) 174, 609, 848, 1179
AclI AACGTT 2 cut(s) 107, 121
AclWI GGATC 4 cut(s) 476, 708, 719, 1085
AcoI YGGCCR 1 cut(s) 233
AcsI RAATTY 1 cut(s) 1122
AcuI CTGAAG 1 cut(s) 644
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 3 cut(s) 418, 742, 831
AfiI CCNNNNNNNGG 2 cut(s) 615, 661
AflIII ACRYGT 2 cut(s) 413, 737
AgsI TTSAA 3 cut(s) 26, 49, 1060
AjnI CCWGG 1 cut(s) 1151
AluBI AGCT 8 cut(s) 11, 402, 652, 658, 773, 779, 1078, 1166
AluI AGCT 8 cut(s) 11, 402, 652, 658, 773, 779, 1078, 1166
Alw26I GTCTC 3 cut(s) 207, 902, 1164
AlwI GGATC 4 cut(s) 476, 708, 719, 1085
AoxI GGCC 1 cut(s) 233
ApoI RAATTY 1 cut(s) 1122
AseI ATTAAT 1 cut(s) 38
AspLEI GCGC 2 cut(s) 16, 266
AsuHPI GGTGA 1 cut(s) 598
BaeI ACNNNNGTAYC 2 cut(s) 759, 792
BanI GGYRCC 1 cut(s) 263
BbvI GCAGC 9 cut(s) 3, 251, 254, 307, 361, 377, 565, 838, 1155
BccI CCATC 5 cut(s) 94, 438, 442, 1010, 1063
BceAI ACGGC 3 cut(s) 251, 308, 591
BciT130I CCWGG 1 cut(s) 1153
BcoDI GTCTC 3 cut(s) 207, 902, 1164
BfaI CTAG 3 cut(s) 135, 629, 843
BfmI CTRYAG 2 cut(s) 723, 774
BfoI RGCGCY 1 cut(s) 267
Bme1390I CCNGG 1 cut(s) 1153
BmiI GGNNCC 2 cut(s) 265, 309
BmrFI CCNGG 1 cut(s) 1153
BmsI GCATC 1 cut(s) 1091
BoxI GACNNNNGTC 1 cut(s) 914
BpmI CTGGAG 1 cut(s) 638
BpuEI CTTGAG 1 cut(s) 1146
BsaAI YACGTR 1 cut(s) 740
BsaBI GATNNNNATC 1 cut(s) 705
BsaHI GRCGYC 1 cut(s) 264
BsaI GGTCTC 1 cut(s) 902
BsaWI WCCGGW 2 cut(s) 379, 809
Bsc4I CCNNNNNNNGG 2 cut(s) 615, 661
Bse3DI GCAATG 2 cut(s) 321, 866
Bse8I GATNNNNATC 1 cut(s) 705
BseBI CCWGG 1 cut(s) 1153
BseGI GGATG 3 cut(s) 190, 430, 1082
BseJI GATNNNNATC 1 cut(s) 705
BseLI CCNNNNNNNGG 2 cut(s) 615, 661
BseMI GCAATG 2 cut(s) 321, 866
BseMII CTCAG 3 cut(s) 212, 218, 375
BseXI GCAGC 9 cut(s) 3, 251, 254, 307, 361, 377, 565, 838, 1155
BseYI CCCAGC 1 cut(s) 1094
BsgI GTGCAG 1 cut(s) 837
Bsh1236I CGCG 1 cut(s) 415
BshFI GGCC 1 cut(s) 235
BshNI GGYRCC 1 cut(s) 263
BsiSI CCGG 2 cut(s) 380, 810
BsiWI CGTACG 2 cut(s) 416, 740
BslFI GGGAC 3 cut(s) 193, 610, 696
BslI CCNNNNNNNGG 2 cut(s) 615, 661
BsmAI GTCTC 3 cut(s) 207, 902, 1164
BsmFI GGGAC 3 cut(s) 193, 610, 696
BsmI GAATGC 1 cut(s) 500
BsnI GGCC 1 cut(s) 235
Bso31I GGTCTC 1 cut(s) 902
Bsp143I GATC 6 cut(s) 481, 486, 700, 711, 959, 1090
BspACI CCGC 4 cut(s) 174, 609, 848, 1179
BspANI GGCC 1 cut(s) 235
BspCNI CTCAG 3 cut(s) 211, 217, 374
BspFNI CGCG 1 cut(s) 415
BspLI GGNNCC 2 cut(s) 265, 309
BspMAI CTGCAG 1 cut(s) 727
BspPI GGATC 4 cut(s) 476, 708, 719, 1085
BspQI GCTCTTC 1 cut(s) 1068
BspT107I GGYRCC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 902
BsrBI CCGCTC 1 cut(s) 848
BsrDI GCAATG 2 cut(s) 321, 866
BssMI GATC 6 cut(s) 481, 486, 700, 711, 959, 1090
BssNI GRCGYC 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 1153
Bst4CI ACNGT 1 cut(s) 601
Bst6I CTCTTC 3 cut(s) 87, 900, 1068
BstACI GRCGYC 1 cut(s) 264
BstAPI GCANNNNNTGC 2 cut(s) 11, 320
BstBAI YACGTR 1 cut(s) 740
BstC8I GCNNGC 4 cut(s) 174, 237, 1080, 1141
BstDEI CTNAG 4 cut(s) 198, 204, 361, 1026
BstF5I GGATG 3 cut(s) 190, 430, 1082
BstFNI CGCG 1 cut(s) 415
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 2 cut(s) 16, 266
BstKTI GATC 6 cut(s) 484, 489, 703, 714, 962, 1093
BstMAI GTCTC 3 cut(s) 207, 902, 1164
BstMBI GATC 6 cut(s) 481, 486, 700, 711, 959, 1090
BstMWI GCNNNNNNNGC 5 cut(s) 11, 320, 371, 374, 722
BstNI CCWGG 1 cut(s) 1153
BstPAI GACNNNNGTC 1 cut(s) 914
BstSCI CCNGG 1 cut(s) 1151
BstSFI CTRYAG 2 cut(s) 723, 774
BstUI CGCG 1 cut(s) 415
BstV1I GCAGC 9 cut(s) 3, 251, 254, 307, 361, 377, 565, 838, 1155
BstX2I RGATCY 1 cut(s) 700
BstXI CCANNNNNNTGG 1 cut(s) 1135
BstYI RGATCY 1 cut(s) 700
BsuRI GGCC 1 cut(s) 235
BtsCI GGATG 3 cut(s) 190, 430, 1082
BtsI GCAGTG 3 cut(s) 696, 1057, 1180
BtsIMutI CAGTG 3 cut(s) 696, 1057, 1180
Cac8I GCNNGC 4 cut(s) 174, 237, 1080, 1141
CfoI GCGC 2 cut(s) 16, 266
Csp6I GTAC 3 cut(s) 417, 741, 830
CspCI CAANNNNNGTGG 2 cut(s) 1024, 1059
CviAII CATG 2 cut(s) 346, 819
CviQI GTAC 3 cut(s) 417, 741, 830
DdeI CTNAG 4 cut(s) 198, 204, 361, 1026
DinI GGCGCC 1 cut(s) 265
DpnI GATC 6 cut(s) 483, 488, 702, 713, 961, 1092
DpnII GATC 6 cut(s) 481, 486, 700, 711, 959, 1090
EaeI YGGCCR 1 cut(s) 233
Eam1104I CTCTTC 3 cut(s) 87, 900, 1068
EarI CTCTTC 3 cut(s) 87, 900, 1068
Eco31I GGTCTC 1 cut(s) 902
Eco57I CTGAAG 1 cut(s) 644
EcoRII CCWGG 1 cut(s) 1151
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
FaeI CATG 2 cut(s) 349, 822
FaiI YATR 9 cut(s) 280, 347, 523, 667, 669, 756, 820, 900, 932
FaqI GGGAC 3 cut(s) 193, 610, 696
FatI CATG 2 cut(s) 345, 818
FauI CCCGC 1 cut(s) 181
FokI GGATG 3 cut(s) 177, 417, 1069
FspBI CTAG 3 cut(s) 135, 629, 843
GlaI GCGC 2 cut(s) 15, 265
GsaI CCCAGC 1 cut(s) 1098
GsuI CTGGAG 1 cut(s) 638
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 1 cut(s) 235
HapII CCGG 2 cut(s) 380, 810
HhaI GCGC 2 cut(s) 16, 266
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 2 cut(s) 349, 822
Hin6I GCGC 2 cut(s) 14, 264
HinP1I GCGC 2 cut(s) 14, 264
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 2 cut(s) 179, 971
HpaII CCGG 2 cut(s) 380, 810
HphI GGTGA 1 cut(s) 598
Hpy166II GTNNAC 4 cut(s) 622, 830, 840, 925
Hpy188I TCNGA 5 cut(s) 469, 486, 711, 884, 957
Hpy188III TCNNGA 1 cut(s) 595
Hpy8I GTNNAC 4 cut(s) 622, 830, 840, 925
Hpy99I CGWCG 1 cut(s) 424
HpyCH4III ACNGT 1 cut(s) 601
HpyCH4IV ACGT 5 cut(s) 107, 121, 422, 464, 739
HpyCH4V TGCA 8 cut(s) 351, 427, 500, 694, 725, 804, 854, 1082
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 320, 371, 374, 722
HpyF3I CTNAG 4 cut(s) 198, 204, 361, 1026
HpySE526I ACGT 5 cut(s) 107, 121, 422, 464, 739
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 2 cut(s) 349, 822
HspAI GCGC 2 cut(s) 14, 264
KasI GGCGCC 1 cut(s) 263
Kzo9I GATC 6 cut(s) 481, 486, 700, 711, 959, 1090
LguI GCTCTTC 1 cut(s) 1068
LmnI GCTCC 3 cut(s) 136, 382, 657
Lsp1109I GCAGC 9 cut(s) 3, 251, 254, 307, 361, 377, 565, 838, 1155
LweI GCATC 1 cut(s) 1091
MaeI CTAG 3 cut(s) 135, 629, 843
MaeII ACGT 5 cut(s) 107, 121, 422, 464, 739
MaeIII GTNAC 3 cut(s) 290, 812, 994
MalI GATC 6 cut(s) 483, 488, 702, 713, 961, 1092
MbiI CCGCTC 1 cut(s) 848
MboI GATC 6 cut(s) 481, 486, 700, 711, 959, 1090
MboII GAAGA 5 cut(s) 74, 438, 917, 1072, 1085
MflI RGATCY 1 cut(s) 700
MluCI AATT 7 cut(s) 44, 253, 495, 873, 999, 1122, 1133
MluI ACGCGT 1 cut(s) 413
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 9 cut(s) 90, 168, 205, 221, 297, 742, 744, 882, 985
MseI TTAA 6 cut(s) 38, 104, 144, 222, 270, 705
MslI CAYNNNNRTG 2 cut(s) 477, 859
MspA1I CMGCKG 2 cut(s) 11, 773
MspI CCGG 2 cut(s) 380, 810
MspR9I CCNGG 1 cut(s) 1153
Mva1269I GAATGC 1 cut(s) 500
MvaI CCWGG 1 cut(s) 1153
MvnI CGCG 1 cut(s) 415
MwoI GCNNNNNNNGC 5 cut(s) 11, 320, 371, 374, 722
NarI GGCGCC 1 cut(s) 264
NdeII GATC 6 cut(s) 481, 486, 700, 711, 959, 1090
NlaIII CATG 2 cut(s) 349, 822
NlaIV GGNNCC 2 cut(s) 265, 309
NmuCI GTSAC 1 cut(s) 290
PciSI GCTCTTC 1 cut(s) 1068
PcsI WCGNNNNNNNCGW 2 cut(s) 73, 419
PctI GAATGC 1 cut(s) 500
PfeI GAWTC 1 cut(s) 971
Pfl23II CGTACG 2 cut(s) 416, 740
PfoI TCCNGGA 1 cut(s) 1151
PleI GAGTC 1 cut(s) 173
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 173
Ppu21I YACGTR 1 cut(s) 740
PshAI GACNNNNGTC 1 cut(s) 914
PshBI ATTAAT 1 cut(s) 38
Psp1406I AACGTT 2 cut(s) 107, 121
Psp6I CCWGG 1 cut(s) 1151
PspFI CCCAGC 1 cut(s) 1094
PspGI CCWGG 1 cut(s) 1151
PspLI CGTACG 2 cut(s) 416, 740
PspN4I GGNNCC 2 cut(s) 265, 309
PstI CTGCAG 1 cut(s) 727
PsuI RGATCY 1 cut(s) 700
PvuII CAGCTG 2 cut(s) 11, 773
RsaI GTAC 3 cut(s) 418, 742, 831
RsaNI GTAC 3 cut(s) 417, 741, 830
RseI CAYNNNNRTG 2 cut(s) 477, 859
SapI GCTCTTC 1 cut(s) 1068
SaqAI TTAA 6 cut(s) 38, 104, 144, 222, 270, 705
Sau3AI GATC 6 cut(s) 481, 486, 700, 711, 959, 1090
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 1 cut(s) 1153
SfaNI GCATC 1 cut(s) 1091
SfcI CTRYAG 2 cut(s) 723, 774
SfoI GGCGCC 1 cut(s) 265
SmiMI CAYNNNNRTG 2 cut(s) 477, 859
SmlI CTYRAG 1 cut(s) 1161
SmoI CTYRAG 1 cut(s) 1161
Sse9I AATT 7 cut(s) 44, 253, 495, 873, 999, 1122, 1133
SsiI CCGC 4 cut(s) 174, 609, 848, 1179
SspDI GGCGCC 1 cut(s) 263
SspI AATATT 2 cut(s) 141, 443
SspMI CTAG 3 cut(s) 135, 629, 843
StyD4I CCNGG 1 cut(s) 1151
TaaI ACNGT 1 cut(s) 601
TaiI ACGT 5 cut(s) 110, 124, 425, 467, 742
TaqI TCGA 1 cut(s) 449
TasI AATT 7 cut(s) 44, 253, 495, 873, 999, 1122, 1133
TauI GCSGC 1 cut(s) 851
TfiI GAWTC 1 cut(s) 971
Tru1I TTAA 6 cut(s) 38, 104, 144, 222, 270, 705
Tru9I TTAA 6 cut(s) 38, 104, 144, 222, 270, 705
TscAI CASTG 3 cut(s) 696, 1057, 1180
TseFI GTSAC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 290
TspDTI ATGAA 1 cut(s) 56
TspGWI ACGGA 1 cut(s) 713
TspRI CASTG 3 cut(s) 696, 1057, 1180
VspI ATTAAT 1 cut(s) 38
XapI RAATTY 1 cut(s) 1122
XcmI CCANNNNNNNNNTGG 1 cut(s) 919
XspI CTAG 3 cut(s) 135, 629, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.