Rroxscaffold_5G00372130

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006404
Physical Location & Seq
Reverse (-)
53596863 .. 53598882
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00372130.1

Sequence Viewer

Length: 1275 bp
ATGTATGAGGAGAAGAATAAAATCTTAACAAAGCTTCTACTATCCTTTCAATTCAAAGTTCGATTACTCGATACAAGTTTCATGGCAACAACTGCGCTGCTCATCGAAGCCAGCCCATTGAAATTGAAGCAAAGCACCCTATTACGCACTTTTCGTCCTTTTCCATCAAAACCAATCAAATCAAGCACAGCAACGTTTAGCTATCAACGTTGCTTTGCTCCTAGATTTAAGAACCCTAAACCTCAATGTTCCTTGCCAGCCGAGAAGACCCACTTCACCGCCGACACTAAATCAGAGAGGGATCTGTCTCAGTGCTTCAATGTTTTCAAAGATAATCGGCCAGCAGCAGCAAACCCTAATTTCTCAGCCGCCGTTAATAAGTTATGGGAAAAGTCTCTCAAGACATTGAGGAAACCGGCAATGGCTGCCGTGCTGTTGGGTTTGTTAGTCGTGTACAACCCCAACTCAGCAGCGGTGGCTGCTCCGGTGAGTCGGTTCGGTGGAAGCTTGACATCCAACAGTAGGACCTCATACTATCCATCTGAAAATTCTTCCACCAGAATCAAAACGTACGACGGATACTCTGAACCGATCTGGGACTGGGGAGTCATTGTCTTTCTTGCATACTTTTTCGGGGGAGGTTCTGTGTGCATGTTACCTTTGCCCAAGTTTTTCCGGGCTGAATTGTTCATTTTGTGGACAGTTGTTATGGTTAATTGGTTTCACTCTCTAGGTCAAACAAGTGTTGTAAAGCTCCAGCTTCAGGTTAATTTGTCGAACGCGGGGCGATCAGTGCAAATGGATCTTAATCGGATGGCTGAAACTGCAGATCCCAATACGCATGAGGGTTTGAGACATGTTTTGAGAGAGACTGCAGTAGCTCTACTTCGGCAACCTGATTATTGCATTTCCGGTTACTCATCTGTGGTTATACCAAAGTTCACTAGAGCGGCTGCGCAGCAATGCTTTGATAAACTTGTTGTCAGAGAGAGGGTTAGATATGACGAAGAGACCATTGTCAGTGTGGACAATATAAAAACTCAAAGCACAGCAAGTCAGAGATCCACTAATGAATCGCACAATGACGATGTAGTGGTTACAGTATTACTTGCTGCTGAAGGGAAGTCTAAGTCGCTCACAATCATTGACAGCAGTGACGTGAAAAGAGTGTTGGAAGAGCTTGCATCCATTGATCCCAGCAAACGAGTATTAGCAGTACAGGTACTGTGGACTCCTCAGTTATCTTCAAATGCAGCAAGCAAATGTATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

424

Amino Acids

47.33

Weight (kDa)

9.51

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 252 - 417 2.2e-42 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 605
Acc16I TGCGCA 1 cut(s) 957
AccBSI CCGCTC 1 cut(s) 950
AccII CGCG 1 cut(s) 782
AciI CCGC 5 cut(s) 279, 369, 473, 782, 950
AclI AACGTT 2 cut(s) 194, 208
AclWI GGATC 5 cut(s) 309, 810, 824, 1056, 1187
AcoI YGGCCR 1 cut(s) 338
AcsI RAATTY 1 cut(s) 547
AcuI CTGAAG 2 cut(s) 746, 1137
AfaI GTAC 4 cut(s) 455, 572, 1218, 1224
AfiI CCNNNNNNNGG 2 cut(s) 522, 763
AflIII ACRYGT 1 cut(s) 856
AgsI TTSAA 7 cut(s) 50, 55, 121, 127, 319, 328, 1248
AjiI CACGTC 1 cut(s) 1159
AjuI GAANNNNNNNTTGG 2 cut(s) 1154, 1186
AluBI AGCT 7 cut(s) 34, 201, 507, 754, 760, 881, 1180
AluI AGCT 7 cut(s) 34, 201, 507, 754, 760, 881, 1180
Alw26I GTCTC 5 cut(s) 312, 399, 847, 863, 1004
AlwI GGATC 5 cut(s) 309, 810, 824, 1056, 1187
AlwNI CAGNNNCTG 1 cut(s) 1225
AoxI GGCC 1 cut(s) 338
ApoI RAATTY 1 cut(s) 547
AseI ATTAAT 1 cut(s) 1269
AspLEI GCGC 2 cut(s) 97, 958
AspS9I GGNCC 1 cut(s) 525
AsuC2I CCSGG 1 cut(s) 677
AsuHPI GGTGA 2 cut(s) 268, 499
AvaII GGWCC 1 cut(s) 525
BarI GAAGNNNNNNTAC 2 cut(s) 870, 902
BbsI GAAGAC 1 cut(s) 272
BccI CCATC 3 cut(s) 172, 547, 808
BceAI ACGGC 2 cut(s) 356, 413
BciVI GTATCC 1 cut(s) 572
BcnI CCSGG 1 cut(s) 677
BcoDI GTCTC 5 cut(s) 312, 399, 847, 863, 1004
BfaI CTAG 3 cut(s) 222, 731, 945
BfmI CTRYAG 2 cut(s) 825, 873
BfuI GTATCC 1 cut(s) 572
Bme1390I CCNGG 1 cut(s) 677
Bme18I GGWCC 1 cut(s) 525
BmgBI CACGTC 1 cut(s) 1159
BmgT120I GGNCC 1 cut(s) 525
BmrFI CCNGG 1 cut(s) 677
BmrI ACTGGG 1 cut(s) 610
BmsI GCATC 1 cut(s) 1193
BmuI ACTGGG 1 cut(s) 610
BoxI GACNNNNGTC 1 cut(s) 1016
BpiI GAAGAC 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 740
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 1 cut(s) 677
BsaBI GATNNNNATC 1 cut(s) 807
BsaI GGTCTC 1 cut(s) 1004
BsaWI WCCGGW 2 cut(s) 484, 911
BsaXI ACNNNNNCTCC 4 cut(s) 597, 627, 630, 660
Bsc4I CCNNNNNNNGG 2 cut(s) 522, 763
Bse118I RCCGGY 1 cut(s) 415
Bse1I ACTGG 1 cut(s) 605
Bse3DI GCAATG 2 cut(s) 426, 968
Bse8I GATNNNNATC 1 cut(s) 807
BseGI GGATG 3 cut(s) 512, 819, 1184
BseJI GATNNNNATC 1 cut(s) 807
BseLI CCNNNNNNNGG 2 cut(s) 522, 763
BseMI GCAATG 2 cut(s) 426, 968
BseMII CTCAG 4 cut(s) 323, 378, 480, 1250
BseNI ACTGG 1 cut(s) 605
BseRI GAGGAG 2 cut(s) 23, 1224
BseYI CCCAGC 1 cut(s) 1196
Bsh1236I CGCG 1 cut(s) 782
BshFI GGCC 1 cut(s) 340
BsiSI CCGG 4 cut(s) 416, 485, 676, 912
BsiWI CGTACG 1 cut(s) 570
BslFI GGGAC 1 cut(s) 611
BslI CCNNNNNNNGG 2 cut(s) 522, 763
BsmAI GTCTC 5 cut(s) 312, 399, 847, 863, 1004
BsmFI GGGAC 1 cut(s) 611
BsnI GGCC 1 cut(s) 340
Bso31I GGTCTC 1 cut(s) 1004
Bsp1407I TGTACA 1 cut(s) 453
Bsp143I GATC 7 cut(s) 301, 591, 788, 802, 829, 1061, 1192
BspACI CCGC 5 cut(s) 279, 369, 473, 782, 950
BspANI GGCC 1 cut(s) 340
BspCNI CTCAG 4 cut(s) 322, 377, 479, 1249
BspFNI CGCG 1 cut(s) 782
BspMAI CTGCAG 2 cut(s) 829, 877
BspPI GGATC 5 cut(s) 309, 810, 824, 1056, 1187
BspQI GCTCTTC 1 cut(s) 1170
BspTNI GGTCTC 1 cut(s) 1004
BsrBI CCGCTC 1 cut(s) 950
BsrDI GCAATG 2 cut(s) 426, 968
BsrFI RCCGGY 1 cut(s) 415
BsrGI TGTACA 1 cut(s) 453
BsrI ACTGG 1 cut(s) 605
BssAI RCCGGY 1 cut(s) 415
BssMI GATC 7 cut(s) 301, 591, 788, 802, 829, 1061, 1192
Bst4CI ACNGT 4 cut(s) 521, 703, 1102, 1227
Bst6I CTCTTC 2 cut(s) 1002, 1170
BstAPI GCANNNNNTGC 2 cut(s) 92, 425
BstAUI TGTACA 1 cut(s) 453
BstC8I GCNNGC 5 cut(s) 112, 258, 342, 1182, 1258
BstDEI CTNAG 5 cut(s) 309, 364, 466, 1128, 1236
BstF5I GGATG 3 cut(s) 512, 819, 1184
BstFNI CGCG 1 cut(s) 782
BstHHI GCGC 2 cut(s) 97, 958
BstKTI GATC 7 cut(s) 304, 594, 791, 805, 832, 1064, 1195
BstMAI GTCTC 5 cut(s) 312, 399, 847, 863, 1004
BstMBI GATC 7 cut(s) 301, 591, 788, 802, 829, 1061, 1192
BstMWI GCNNNNNNNGC 6 cut(s) 92, 425, 476, 479, 793, 824
BstNSI RCATGY 2 cut(s) 655, 860
BstPAI GACNNNNGTC 1 cut(s) 1016
BstSCI CCNGG 1 cut(s) 675
BstSFI CTRYAG 2 cut(s) 825, 873
BstUI CGCG 1 cut(s) 782
BstV2I GAAGAC 1 cut(s) 272
BstX2I RGATCY 4 cut(s) 301, 802, 829, 1061
BstYI RGATCY 4 cut(s) 301, 802, 829, 1061
BsuI GTATCC 1 cut(s) 572
BsuRI GGCC 1 cut(s) 340
BtrI CACGTC 1 cut(s) 1159
BtsCI GGATG 3 cut(s) 512, 819, 1184
BtsI GCAGTG 1 cut(s) 1159
BtsIMutI CAGTG 4 cut(s) 317, 798, 1027, 1159
Cac8I GCNNGC 5 cut(s) 112, 258, 342, 1182, 1258
CaiI CAGNNNCTG 1 cut(s) 1225
CfoI GCGC 2 cut(s) 97, 958
Cfr10I RCCGGY 1 cut(s) 415
Cfr13I GGNCC 1 cut(s) 525
Csp6I GTAC 4 cut(s) 454, 571, 1217, 1223
CviAII CATG 4 cut(s) 82, 652, 842, 857
CviQI GTAC 4 cut(s) 454, 571, 1217, 1223
DdeI CTNAG 5 cut(s) 309, 364, 466, 1128, 1236
DpnI GATC 7 cut(s) 303, 593, 790, 804, 831, 1063, 1194
DpnII GATC 7 cut(s) 301, 591, 788, 802, 829, 1061, 1192
DrdI GACNNNNNNGTC 1 cut(s) 605
DseDI GACNNNNNNGTC 1 cut(s) 605
EaeI YGGCCR 1 cut(s) 338
Eam1104I CTCTTC 2 cut(s) 1002, 1170
EarI CTCTTC 2 cut(s) 1002, 1170
Eco31I GGTCTC 1 cut(s) 1004
Eco47I GGWCC 1 cut(s) 525
Eco57I CTGAAG 2 cut(s) 746, 1137
EcoO109I RGGNCCY 1 cut(s) 525
FaeI CATG 4 cut(s) 85, 655, 845, 860
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FaqI GGGAC 1 cut(s) 611
FatI CATG 4 cut(s) 81, 651, 841, 856
FauI CCCGC 1 cut(s) 775
FokI GGATG 3 cut(s) 499, 826, 1171
FspBI CTAG 3 cut(s) 222, 731, 945
FspI TGCGCA 1 cut(s) 957
GlaI GCGC 2 cut(s) 96, 957
GsaI CCCAGC 1 cut(s) 1200
GsuI CTGGAG 1 cut(s) 740
HaeIII GGCC 1 cut(s) 340
HapII CCGG 4 cut(s) 416, 485, 676, 912
HhaI GCGC 2 cut(s) 97, 958
Hin1II CATG 4 cut(s) 85, 655, 845, 860
Hin6I GCGC 2 cut(s) 95, 956
HinP1I GCGC 2 cut(s) 95, 956
HindIII AAGCTT 2 cut(s) 32, 505
HinfI GANTC 5 cut(s) 490, 561, 606, 1073, 1231
HpaII CCGG 4 cut(s) 416, 485, 676, 912
HphI GGTGA 2 cut(s) 268, 499
Hpy166II GTNNAC 5 cut(s) 454, 699, 942, 1027, 1230
Hpy188I TCNGA 6 cut(s) 295, 544, 586, 813, 986, 1059
Hpy188III TCNNGA 1 cut(s) 400
Hpy8I GTNNAC 5 cut(s) 454, 699, 942, 1027, 1230
Hpy99I CGWCG 1 cut(s) 578
HpyAV CCTTC 1 cut(s) 1112
HpyCH4III ACNGT 4 cut(s) 521, 703, 1102, 1227
HpyCH4IV ACGT 4 cut(s) 194, 208, 569, 1158
HpyCH4V TGCA 8 cut(s) 623, 651, 796, 827, 875, 906, 1184, 1253
HpyF10VI GCNNNNNNNGC 6 cut(s) 92, 425, 476, 479, 793, 824
HpyF3I CTNAG 5 cut(s) 309, 364, 466, 1128, 1236
HpySE526I ACGT 4 cut(s) 194, 208, 569, 1158
Hsp92II CATG 4 cut(s) 85, 655, 845, 860
HspAI GCGC 2 cut(s) 95, 956
Kzo9I GATC 7 cut(s) 301, 591, 788, 802, 829, 1061, 1192
LguI GCTCTTC 1 cut(s) 1170
LmnI GCTCC 3 cut(s) 223, 487, 759
LweI GCATC 1 cut(s) 1193
MaeI CTAG 3 cut(s) 222, 731, 945
MaeII ACGT 4 cut(s) 194, 208, 569, 1158
MaeIII GTNAC 4 cut(s) 654, 914, 1096, 1154
MalI GATC 7 cut(s) 303, 593, 790, 804, 831, 1063, 1194
MbiI CCGCTC 1 cut(s) 950
MboI GATC 7 cut(s) 301, 591, 788, 802, 829, 1061, 1192
MboII GAAGA 6 cut(s) 25, 277, 543, 1019, 1187, 1236
MflI RGATCY 4 cut(s) 301, 802, 829, 1061
MluCI AATT 8 cut(s) 50, 122, 358, 547, 683, 715, 769, 1270
MlyI GAGTC 3 cut(s) 499, 615, 1225
MmeI TCCRAC 2 cut(s) 540, 1152
MnlI CCTC 8 cut(s) 252, 291, 402, 538, 632, 838, 984, 1245
MseI TTAA 8 cut(s) 26, 228, 375, 714, 768, 807, 1269, 1273
MspA1I CMGCKG 1 cut(s) 473
MspI CCGG 4 cut(s) 416, 485, 676, 912
MspR9I CCNGG 1 cut(s) 677
MvnI CGCG 1 cut(s) 782
MwoI GCNNNNNNNGC 6 cut(s) 92, 425, 476, 479, 793, 824
NciI CCSGG 1 cut(s) 677
NdeII GATC 7 cut(s) 301, 591, 788, 802, 829, 1061, 1192
NlaIII CATG 4 cut(s) 85, 655, 845, 860
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 1 cut(s) 1154
NsbI TGCGCA 1 cut(s) 957
NspI RCATGY 2 cut(s) 655, 860
PacI TTAATTAA 1 cut(s) 1273
PciI ACATGT 1 cut(s) 856
PciSI GCTCTTC 1 cut(s) 1170
PcsI WCGNNNNNNNCGW 1 cut(s) 151
PfeI GAWTC 2 cut(s) 561, 1073
Pfl23II CGTACG 1 cut(s) 570
PleI GAGTC 3 cut(s) 498, 614, 1225
PpsI GAGTC 3 cut(s) 498, 614, 1225
PpuMI RGGWCCY 1 cut(s) 525
PscI ACATGT 1 cut(s) 856
PshAI GACNNNNGTC 1 cut(s) 1016
PshBI ATTAAT 1 cut(s) 1269
Psp1406I AACGTT 2 cut(s) 194, 208
Psp5II RGGWCCY 1 cut(s) 525
PspFI CCCAGC 1 cut(s) 1196
PspLI CGTACG 1 cut(s) 570
PspPI GGNCC 1 cut(s) 525
PspPPI RGGWCCY 1 cut(s) 525
PstI CTGCAG 2 cut(s) 829, 877
PstNI CAGNNNCTG 1 cut(s) 1225
PsuI RGATCY 4 cut(s) 301, 802, 829, 1061
RsaI GTAC 4 cut(s) 455, 572, 1218, 1224
RsaNI GTAC 4 cut(s) 454, 571, 1217, 1223
SapI GCTCTTC 1 cut(s) 1170
SaqAI TTAA 8 cut(s) 26, 228, 375, 714, 768, 807, 1269, 1273
Sau3AI GATC 7 cut(s) 301, 591, 788, 802, 829, 1061, 1192
Sau96I GGNCC 1 cut(s) 525
SchI GAGTC 3 cut(s) 499, 615, 1225
ScrFI CCNGG 1 cut(s) 677
SfaNI GCATC 1 cut(s) 1193
SfcI CTRYAG 2 cut(s) 825, 873
SinI GGWCC 1 cut(s) 525
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 8 cut(s) 50, 122, 358, 547, 683, 715, 769, 1270
SsiI CCGC 5 cut(s) 279, 369, 473, 782, 950
SspMI CTAG 3 cut(s) 222, 731, 945
StyD4I CCNGG 1 cut(s) 675
TaaI ACNGT 4 cut(s) 521, 703, 1102, 1227
TaiI ACGT 4 cut(s) 197, 211, 572, 1161
TaqI TCGA 4 cut(s) 61, 69, 105, 776
TasI AATT 8 cut(s) 50, 122, 358, 547, 683, 715, 769, 1270
TatI WGTACW 2 cut(s) 453, 1216
TauI GCSGC 2 cut(s) 371, 953
TfiI GAWTC 2 cut(s) 561, 1073
Tru1I TTAA 8 cut(s) 26, 228, 375, 714, 768, 807, 1269, 1273
Tru9I TTAA 8 cut(s) 26, 228, 375, 714, 768, 807, 1269, 1273
TscAI CASTG 4 cut(s) 317, 798, 1027, 1159
TseFI GTSAC 1 cut(s) 1154
Tsp45I GTSAC 1 cut(s) 1154
TspDTI ATGAA 3 cut(s) 70, 679, 1086
TspGWI ACGGA 1 cut(s) 591
TspRI CASTG 4 cut(s) 317, 798, 1027, 1159
VpaK11BI GGWCC 1 cut(s) 525
VspI ATTAAT 1 cut(s) 1269
XapI RAATTY 1 cut(s) 547
XceI RCATGY 2 cut(s) 655, 860
XcmI CCANNNNNNNNNTGG 1 cut(s) 1021
XspI CTAG 3 cut(s) 222, 731, 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.