Rroxscaffold_2G00078580

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006404
Physical Location & Seq
Forward (+)
1844825 .. 1863172
18348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00078580.1

Sequence Viewer

Length: 786 bp
ATGGCAACAGCTGCGCTGCTCATCGAAGCCAACCCATTAATGAAATTGAAGCAAAACACCCTATTACGCACTTTTCGTCCTCTTCCATCAAAACCAGTCACATTAACGTTAAGCAATCAACGTTGCTTTGCTCCCAGAATATTTAAGAACCCTATACCTCAATGTTTCTTGCCCTCCGACTCTAAATCAGGCCATCCCTCTCAGTCACAGTTCCACGATATTCTTAAAGACAATTGGCCAGCGGCAGCAAACCCTAATTTCACAGTCGCCCTTAATAAGTTATGGGAAAAGTGTCACAAGGCATTGAGGAAACCAGCAATGGCTGCCGTGCTATTCGGTTTGTTAGTCATGTACAACCCCAAGTCAGCAGCGTTGGCTGCTCCGGTGGGTCGGGTCGGTGGAAGCTTGACACCCACGTGTACGACGTCGCACCGTCCATCTAAATATTCTTCGACCACAATCAAAACGTCCGACCACTACGATGATCCGATCTGGGAGTTTGCAGTCTTATGGTTCACTATTTTCTGCTGTTACGCTGGATTTTTTGCTTGGTCTTTCGCCTCTTTGAAGTTTAACAAGGCTGCCCTGTTTACTTTCGGGACAGTTTTCATCGCTATTTGGGTTCACTCTCTAGGTCAAACAAGTGTTGTCAATCTCCAGCTTCAGGTTACTATATGGAACACGGGACGAGCAGTGCAAACGGATCTTAATCGGATCGCTGAAACTGCAGATACCTCTACACCTACGGGTTTGAGATACAGCAGCAGCTCTATCTCGGCAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

261

Amino Acids

28.79

Weight (kDa)

9.72

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 219 - 254 6.7e-06 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 428
AciI CCGC 1 cut(s) 242
AclI AACGTT 2 cut(s) 107, 121
AclWI GGATC 3 cut(s) 479, 711, 722
AcoI YGGCCR 1 cut(s) 236
AcuI CTGAAG 1 cut(s) 647
AcvI CACGTG 1 cut(s) 417
AcyI GRCGYC 1 cut(s) 425
AfaI GTAC 2 cut(s) 353, 421
AfiI CCNNNNNNNGG 1 cut(s) 664
AflIII ACRYGT 1 cut(s) 416
AgsI TTSAA 2 cut(s) 49, 568
AleI CACNNNNGTG 1 cut(s) 415
AluBI AGCT 4 cut(s) 11, 405, 661, 768
AluI AGCT 4 cut(s) 11, 405, 661, 768
AlwI GGATC 3 cut(s) 479, 711, 722
AoxI GGCC 2 cut(s) 190, 236
ApeKI GCWGC 9 cut(s) 11, 16, 245, 323, 368, 377, 581, 762, 765
AseI ATTAAT 1 cut(s) 38
AspLEI GCGC 1 cut(s) 16
BalI TGGCCA 1 cut(s) 238
BbrPI CACGTG 1 cut(s) 417
BbvI GCAGC 8 cut(s) 3, 257, 310, 364, 380, 568, 774, 777
BccI CCATC 3 cut(s) 94, 201, 445
BceAI ACGGC 1 cut(s) 311
BfaI CTAG 1 cut(s) 632
BfmI CTRYAG 1 cut(s) 726
BpmI CTGGAG 1 cut(s) 641
BsaAI YACGTR 1 cut(s) 417
BsaBI GATNNNNATC 1 cut(s) 708
BsaHI GRCGYC 1 cut(s) 425
BsaWI WCCGGW 1 cut(s) 382
Bsc4I CCNNNNNNNGG 1 cut(s) 664
Bse1I ACTGG 1 cut(s) 95
Bse3DI GCAATG 1 cut(s) 324
Bse8I GATNNNNATC 1 cut(s) 708
BseGI GGATG 1 cut(s) 193
BseJI GATNNNNATC 1 cut(s) 708
BseLI CCNNNNNNNGG 1 cut(s) 664
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 1 cut(s) 95
BseXI GCAGC 8 cut(s) 3, 257, 310, 364, 380, 568, 774, 777
BshFI GGCC 2 cut(s) 192, 238
BsiSI CCGG 1 cut(s) 383
BslFI GGGAC 2 cut(s) 613, 699
BslI CCNNNNNNNGG 1 cut(s) 664
BsmFI GGGAC 2 cut(s) 613, 699
BsnI GGCC 2 cut(s) 192, 238
Bsp1407I TGTACA 1 cut(s) 351
Bsp143I GATC 4 cut(s) 484, 489, 703, 714
BspACI CCGC 1 cut(s) 242
BspANI GGCC 2 cut(s) 192, 238
BspCNI CTCAG 1 cut(s) 214
BspMAI CTGCAG 1 cut(s) 730
BspPI GGATC 3 cut(s) 479, 711, 722
BsrDI GCAATG 1 cut(s) 324
BsrGI TGTACA 1 cut(s) 351
BsrI ACTGG 1 cut(s) 95
BssMI GATC 4 cut(s) 484, 489, 703, 714
BssNI GRCGYC 1 cut(s) 425
Bst4CI ACNGT 4 cut(s) 210, 265, 434, 604
Bst6I CTCTTC 1 cut(s) 87
BstACI GRCGYC 1 cut(s) 425
BstAPI GCANNNNNTGC 2 cut(s) 11, 323
BstAUI TGTACA 1 cut(s) 351
BstBAI YACGTR 1 cut(s) 417
BstC8I GCNNGC 1 cut(s) 240
BstDEI CTNAG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 193
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 4 cut(s) 487, 492, 706, 717
BstMBI GATC 4 cut(s) 484, 489, 703, 714
BstMWI GCNNNNNNNGC 5 cut(s) 11, 323, 374, 377, 725
BstSFI CTRYAG 1 cut(s) 726
BstV1I GCAGC 8 cut(s) 3, 257, 310, 364, 380, 568, 774, 777
BstX2I RGATCY 1 cut(s) 703
BstYI RGATCY 1 cut(s) 703
BsuRI GGCC 2 cut(s) 192, 238
BtgZI GCGATG 1 cut(s) 595
BtsCI GGATG 1 cut(s) 193
BtsI GCAGTG 1 cut(s) 699
BtsIMutI CAGTG 1 cut(s) 699
Cac8I GCNNGC 1 cut(s) 240
CfoI GCGC 1 cut(s) 16
Csp6I GTAC 2 cut(s) 352, 420
CviAII CATG 1 cut(s) 349
CviQI GTAC 2 cut(s) 352, 420
DdeI CTNAG 1 cut(s) 201
DpnI GATC 4 cut(s) 486, 491, 705, 716
DpnII GATC 4 cut(s) 484, 489, 703, 714
EaeI YGGCCR 1 cut(s) 236
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco57I CTGAAG 1 cut(s) 647
Eco72I CACGTG 1 cut(s) 417
FaeI CATG 1 cut(s) 352
FaiI YATR 6 cut(s) 155, 283, 350, 511, 674, 676
FaqI GGGAC 2 cut(s) 613, 699
FatI CATG 1 cut(s) 348
FokI GGATG 1 cut(s) 180
FspBI CTAG 1 cut(s) 632
GlaI GCGC 1 cut(s) 15
GsuI CTGGAG 1 cut(s) 641
HaeIII GGCC 2 cut(s) 192, 238
HapII CCGG 1 cut(s) 383
HhaI GCGC 1 cut(s) 16
Hin1I GRCGYC 1 cut(s) 425
Hin1II CATG 1 cut(s) 352
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HindIII AAGCTT 1 cut(s) 403
HinfI GANTC 1 cut(s) 179
HpaII CCGG 1 cut(s) 383
Hpy166II GTNNAC 4 cut(s) 420, 516, 591, 625
Hpy188I TCNGA 4 cut(s) 178, 472, 489, 714
Hpy188III TCNNGA 1 cut(s) 598
Hpy8I GTNNAC 4 cut(s) 420, 516, 591, 625
Hpy99I CGWCG 2 cut(s) 427, 430
HpyCH4III ACNGT 4 cut(s) 210, 265, 434, 604
HpyCH4IV ACGT 5 cut(s) 107, 121, 416, 425, 467
HpyCH4V TGCA 3 cut(s) 503, 697, 728
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 323, 374, 377, 725
HpyF3I CTNAG 1 cut(s) 201
HpySE526I ACGT 5 cut(s) 107, 121, 416, 425, 467
Hsp92I GRCGYC 1 cut(s) 425
Hsp92II CATG 1 cut(s) 352
HspAI GCGC 1 cut(s) 14
Kzo9I GATC 4 cut(s) 484, 489, 703, 714
LmnI GCTCC 2 cut(s) 136, 385
Lsp1109I GCAGC 8 cut(s) 3, 257, 310, 364, 380, 568, 774, 777
MaeI CTAG 1 cut(s) 632
MaeII ACGT 5 cut(s) 107, 121, 416, 425, 467
MaeIII GTNAC 5 cut(s) 97, 204, 293, 530, 667
MalI GATC 4 cut(s) 486, 491, 705, 716
MboI GATC 4 cut(s) 484, 489, 703, 714
MboII GAAGA 2 cut(s) 74, 441
MfeI CAATTG 1 cut(s) 232
MflI RGATCY 1 cut(s) 703
MlsI TGGCCA 1 cut(s) 238
MluCI AATT 3 cut(s) 44, 232, 256
MluNI TGGCCA 1 cut(s) 238
MlyI GAGTC 1 cut(s) 173
MmeI TCCRAC 2 cut(s) 201, 495
MnlI CCTC 7 cut(s) 90, 168, 184, 208, 300, 571, 745
Mox20I TGGCCA 1 cut(s) 238
MscI TGGCCA 1 cut(s) 238
MseI TTAA 8 cut(s) 38, 104, 110, 144, 225, 273, 573, 708
MslI CAYNNNNRTG 2 cut(s) 415, 480
Msp20I TGGCCA 1 cut(s) 238
MspA1I CMGCKG 2 cut(s) 11, 242
MspI CCGG 1 cut(s) 383
MunI CAATTG 1 cut(s) 232
MwoI GCNNNNNNNGC 5 cut(s) 11, 323, 374, 377, 725
NdeII GATC 4 cut(s) 484, 489, 703, 714
NlaIII CATG 1 cut(s) 352
NmeAIII GCCGAG 1 cut(s) 755
NmuCI GTSAC 3 cut(s) 97, 204, 293
OliI CACNNNNGTG 1 cut(s) 415
PcsI WCGNNNNNNNCGW 2 cut(s) 73, 422
PleI GAGTC 1 cut(s) 173
PmaCI CACGTG 1 cut(s) 417
PmlI CACGTG 1 cut(s) 417
PpsI GAGTC 1 cut(s) 173
Ppu21I YACGTR 1 cut(s) 417
PshBI ATTAAT 1 cut(s) 38
Psp1406I AACGTT 2 cut(s) 107, 121
PspCI CACGTG 1 cut(s) 417
PstI CTGCAG 1 cut(s) 730
PsuI RGATCY 1 cut(s) 703
PvuII CAGCTG 1 cut(s) 11
RsaI GTAC 2 cut(s) 353, 421
RsaNI GTAC 2 cut(s) 352, 420
RseI CAYNNNNRTG 2 cut(s) 415, 480
SaqAI TTAA 8 cut(s) 38, 104, 110, 144, 225, 273, 573, 708
Sau3AI GATC 4 cut(s) 484, 489, 703, 714
SchI GAGTC 1 cut(s) 173
SfcI CTRYAG 1 cut(s) 726
SmiMI CAYNNNNRTG 2 cut(s) 415, 480
Sse9I AATT 3 cut(s) 44, 232, 256
SsiI CCGC 1 cut(s) 242
SspI AATATT 2 cut(s) 141, 446
SspMI CTAG 1 cut(s) 632
TaaI ACNGT 4 cut(s) 210, 265, 434, 604
TaiI ACGT 5 cut(s) 110, 124, 419, 428, 470
TaqI TCGA 2 cut(s) 24, 452
TasI AATT 3 cut(s) 44, 232, 256
TatI WGTACW 1 cut(s) 351
TauI GCSGC 1 cut(s) 245
Tru1I TTAA 8 cut(s) 38, 104, 110, 144, 225, 273, 573, 708
Tru9I TTAA 8 cut(s) 38, 104, 110, 144, 225, 273, 573, 708
TscAI CASTG 1 cut(s) 699
TseFI GTSAC 3 cut(s) 97, 204, 293
TseI GCWGC 9 cut(s) 11, 16, 245, 323, 368, 377, 581, 762, 765
Tsp45I GTSAC 3 cut(s) 97, 204, 293
TspDTI ATGAA 2 cut(s) 56, 598
TspGWI ACGGA 1 cut(s) 716
TspRI CASTG 1 cut(s) 699
VspI ATTAAT 1 cut(s) 38
XspI CTAG 1 cut(s) 632
ZraI GACGTC 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.