Rroxscaffold_1G00045090

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005528
Physical Location & Seq
Forward (+)
64145339 .. 64146746
1408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00045090.1

Sequence Viewer

Length: 495 bp
ATGGGAAGTAGTATCTTTTCACCAGCCACAACACAGGTTGGAGCAACTGAGAAAATGGGGATGTGGGATATCGTTTCTGGGGCATCCGATTCATTGAAACGGAACGTGCCGGATCAAACGCCCGTTACCAAGGTCGCCAAGAACGTCTTCTCCACCGCTTACGGATTTGGCTCCGCCGCCGTTACCAAGGTCGACGGCGCCGTCAGGGATAAAATGATCCCCTACTTTCGAAACGAGCAAGTGTGGTCCAAGATCTCCCTATATGGCCCGATCCTCCTTAGAAACGGAGCCTCTGAGTTCTCTAAATTAATTCCAGGTGGAAATACATTTTATAACTTTGCTGCCGGATGCTTCTGTGACTATAACAATTCTAATAAACAAAAAGTGGATGTGAAAGAATTGCTGGCTAAAGTATCGAGACTAGAGAAAGAATTCGGTGAATTCCAGAAAAGGAAGACCAAGAAGGCTTGGTGGCAGGAAATTCTGAAGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

164

Amino Acids

18.27

Weight (kDa)

9.59

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 333
AasI GACNNNNNNGTC 1 cut(s) 200
AccB1I GGYRCC 1 cut(s) 197
AccI GTMKAC 1 cut(s) 192
AciI CCGC 3 cut(s) 156, 174, 177
AclWI GGATC 3 cut(s) 120, 211, 265
AcsI RAATTY 3 cut(s) 431, 440, 480
AcyI GRCGYC 1 cut(s) 198
AgsI TTSAA 1 cut(s) 97
AjnI CCWGG 1 cut(s) 313
AjuI GAANNNNNNNTTGG 2 cut(s) 131, 163
AloI GAACNNNNNNTCC 2 cut(s) 134, 166
Alw26I GTCTC 1 cut(s) 412
AlwI GGATC 3 cut(s) 120, 211, 265
AoxI GGCC 1 cut(s) 265
ApeKI GCWGC 1 cut(s) 341
ApoI RAATTY 3 cut(s) 431, 440, 480
AseI ATTAAT 1 cut(s) 308
Asp700I GAANNNNTTC 2 cut(s) 146, 431
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 2 cut(s) 246, 266
AsuHPI GGTGA 2 cut(s) 12, 449
AsuII TTCGAA 1 cut(s) 229
AvaII GGWCC 1 cut(s) 246
BanI GGYRCC 1 cut(s) 197
BbsI GAAGAC 2 cut(s) 139, 461
BbvI GCAGC 1 cut(s) 328
BceAI ACGGC 3 cut(s) 164, 185, 211
BciT130I CCWGG 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 412
BfaI CTAG 1 cut(s) 422
BfoI RGCGCY 1 cut(s) 201
BglII AGATCT 1 cut(s) 252
BisI GCNGC 2 cut(s) 177, 342
BlsI GCNGC 2 cut(s) 178, 343
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 246
BmgT120I GGNCC 2 cut(s) 246, 266
BmiI GGNNCC 3 cut(s) 172, 199, 289
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 2 cut(s) 92, 338
BpiI GAAGAC 2 cut(s) 139, 461
Bpu14I TTCGAA 1 cut(s) 229
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 129, 186
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 2 cut(s) 129, 186
BseGI GGATG 4 cut(s) 66, 83, 353, 394
BseMII CTCAG 2 cut(s) 39, 285
BseXI GCAGC 1 cut(s) 328
BshFI GGCC 1 cut(s) 267
BshNI GGYRCC 1 cut(s) 197
BsiSI CCGG 2 cut(s) 110, 345
BsmAI GTCTC 1 cut(s) 412
BsnI GGCC 1 cut(s) 267
Bsp119I TTCGAA 1 cut(s) 229
Bsp143I GATC 4 cut(s) 112, 216, 252, 270
BspACI CCGC 3 cut(s) 156, 174, 177
BspANI GGCC 1 cut(s) 267
BspCNI CTCAG 2 cut(s) 40, 286
BspLI GGNNCC 3 cut(s) 172, 199, 289
BspPI GGATC 3 cut(s) 120, 211, 265
BspT104I TTCGAA 1 cut(s) 229
BspT107I GGYRCC 1 cut(s) 197
BssECI CCNNGG 2 cut(s) 129, 186
BssMI GATC 4 cut(s) 112, 216, 252, 270
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 2 cut(s) 129, 186
Bst2UI CCWGG 1 cut(s) 315
BstACI GRCGYC 1 cut(s) 198
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 1 cut(s) 405
BstDEI CTNAG 3 cut(s) 48, 278, 294
BstF5I GGATG 4 cut(s) 66, 83, 353, 394
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 4 cut(s) 115, 219, 255, 273
BstMAI GTCTC 1 cut(s) 412
BstMBI GATC 4 cut(s) 112, 216, 252, 270
BstNI CCWGG 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 313
BstV1I GCAGC 1 cut(s) 328
BstV2I GAAGAC 2 cut(s) 139, 461
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BsuRI GGCC 1 cut(s) 267
BtsCI GGATG 4 cut(s) 66, 83, 353, 394
Cac8I GCNNGC 1 cut(s) 405
CfoI GCGC 1 cut(s) 200
Cfr13I GGNCC 2 cut(s) 246, 266
CviJI RGCY 6 cut(s) 26, 171, 267, 290, 407, 467
CviKI_1 RGCY 6 cut(s) 26, 171, 267, 290, 407, 467
DdeI CTNAG 3 cut(s) 48, 278, 294
DinI GGCGCC 1 cut(s) 199
DpnI GATC 4 cut(s) 114, 218, 254, 272
DpnII GATC 4 cut(s) 112, 216, 252, 270
DrdI GACNNNNNNGTC 1 cut(s) 200
DseDI GACNNNNNNGTC 1 cut(s) 200
EciI GGCGGA 1 cut(s) 163
Eco130I CCWWGG 2 cut(s) 129, 186
Eco32I GATATC 1 cut(s) 70
Eco47I GGWCC 1 cut(s) 246
EcoRI GAATTC 2 cut(s) 431, 440
EcoRII CCWGG 1 cut(s) 313
EcoRV GATATC 1 cut(s) 70
EcoT14I CCWWGG 2 cut(s) 129, 186
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
ErhI CCWWGG 2 cut(s) 129, 186
FaiI YATR 4 cut(s) 262, 264, 333, 363
FalI AAGNNNNNCTT 2 cut(s) 131, 163
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 2 cut(s) 177, 342
FokI GGATG 4 cut(s) 70, 73, 360, 401
Fsp4HI GCNGC 2 cut(s) 177, 342
FspBI CTAG 1 cut(s) 422
GlaI GCGC 1 cut(s) 199
GluI GCNGC 2 cut(s) 177, 342
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 1 cut(s) 267
HapII CCGG 2 cut(s) 110, 345
HhaI GCGC 1 cut(s) 200
Hin1I GRCGYC 1 cut(s) 198
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HinfI GANTC 1 cut(s) 89
HpaII CCGG 2 cut(s) 110, 345
HphI GGTGA 2 cut(s) 12, 449
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 3 cut(s) 88, 295, 486
Hpy188III TCNNGA 2 cut(s) 417, 445
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 1 cut(s) 457
HpyCH4IV ACGT 2 cut(s) 105, 144
HpyF3I CTNAG 3 cut(s) 48, 278, 294
HpySE526I ACGT 2 cut(s) 105, 144
Hsp92I GRCGYC 1 cut(s) 198
HspAI GCGC 1 cut(s) 198
KasI GGCGCC 1 cut(s) 197
Kzo9I GATC 4 cut(s) 112, 216, 252, 270
LmnI GCTCC 3 cut(s) 41, 176, 287
Lsp1109I GCAGC 1 cut(s) 328
LweI GCATC 2 cut(s) 92, 338
MaeI CTAG 1 cut(s) 422
MaeII ACGT 2 cut(s) 105, 144
MaeIII GTNAC 3 cut(s) 124, 181, 356
MalI GATC 4 cut(s) 114, 218, 254, 272
MboI GATC 4 cut(s) 112, 216, 252, 270
MboII GAAGA 2 cut(s) 139, 466
MflI RGATCY 1 cut(s) 252
MluCI AATT 7 cut(s) 305, 309, 367, 398, 431, 440, 480
Mly113I GGCGCC 1 cut(s) 198
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 2 cut(s) 284, 301
MroXI GAANNNNTTC 2 cut(s) 146, 431
MseI TTAA 1 cut(s) 308
MspI CCGG 2 cut(s) 110, 345
MspR9I CCNGG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 315
NarI GGCGCC 1 cut(s) 198
NdeII GATC 4 cut(s) 112, 216, 252, 270
NlaIV GGNNCC 3 cut(s) 172, 199, 289
NmuCI GTSAC 1 cut(s) 356
NspV TTCGAA 1 cut(s) 229
PcsI WCGNNNNNNNCGW 2 cut(s) 141, 198
PdmI GAANNNNTTC 2 cut(s) 146, 431
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 2 cut(s) 178, 343
PluTI GGCGCC 1 cut(s) 201
PshBI ATTAAT 1 cut(s) 308
PsiI TTATAA 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspN4I GGNNCC 3 cut(s) 172, 199, 289
PspPI GGNCC 2 cut(s) 246, 266
PsuI RGATCY 1 cut(s) 252
SalI GTCGAC 1 cut(s) 191
SaqAI TTAA 1 cut(s) 308
SatI GCNGC 2 cut(s) 177, 342
Sau3AI GATC 4 cut(s) 112, 216, 252, 270
Sau96I GGNCC 2 cut(s) 246, 266
ScrFI CCNGG 1 cut(s) 315
SetI ASST 6 cut(s) 39, 108, 135, 147, 192, 319
SfaNI GCATC 2 cut(s) 92, 338
SfoI GGCGCC 1 cut(s) 199
SfuI TTCGAA 1 cut(s) 229
SinI GGWCC 1 cut(s) 246
Sse9I AATT 7 cut(s) 305, 309, 367, 398, 431, 440, 480
SsiI CCGC 3 cut(s) 156, 174, 177
SspDI GGCGCC 1 cut(s) 197
SspMI CTAG 1 cut(s) 422
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 2 cut(s) 129, 186
TaiI ACGT 2 cut(s) 108, 147
TaqI TCGA 3 cut(s) 192, 229, 416
TasI AATT 7 cut(s) 305, 309, 367, 398, 431, 440, 480
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TseFI GTSAC 1 cut(s) 356
TseI GCWGC 1 cut(s) 341
Tsp45I GTSAC 1 cut(s) 356
TspDTI ATGAA 1 cut(s) 81
TspGWI ACGGA 3 cut(s) 115, 177, 300
VpaK11BI GGWCC 1 cut(s) 246
VspI ATTAAT 1 cut(s) 308
XapI RAATTY 3 cut(s) 431, 440, 480
XmiI GTMKAC 1 cut(s) 192
XmnI GAANNNNTTC 2 cut(s) 146, 431
XspI CTAG 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.