Rroxscaffold_2G00100390

Ethylene-responsive nuclear protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002810
Physical Location & Seq
Reverse (-)
22266711 .. 22267448
738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00100390.1

Sequence Viewer

Length: 738 bp
ATGGCCGGCGAAGATGATCATGGTGTTTCGGAAAACACTGATTCTCGGATTTCGGATGCGGAAAGTTCTGCTGAGGAATCGGCCGGTGGCAGGTGTAGATATTTGACTTCGGTTATTGTGGCGGTGGCGAAGGTGTTTGCGGTGGTGGTTTTGGCTATGTGCACCACCAAGCTCGCCGTTGGGATCACATTGTCAGCTTTTCTGCTTCTGCTTTTGTTTATGGAAGGTAAGCACTTTTCTTGTTTAATGAAAACTTGCTCGAATGCAAAATCGGCTTTGCAATATATGGCTAAAAGGATTGGCTATGCAATATTGGTTTTGAGGAGTGATGTGGTTGTGAAAGAAAAGAGTGATGGTGTTGTGTTTGAATTGGTTGAACCTGGGGTTGAATCAGACGTTGAATCAGAATCTTCAATTGAAGAGATTGAGGTTGTGGAGGTTAAGAGTAGTGGTAGTGTGTGTATTGATCAAGGTAATAGTCATATGAGCTTGATTGAGTATTTGGTTGTGGATGATGAGAAGTGGAAGAAGATTGAGGCGGGTGATCATACTGATGACTTATCGGTTTCGGTTGGTAGGAAGAGTAAGGGTAGAAAGAAGTCTGTGCCTGAGATGGGAGCAAAGTTTATCAAAAAGTTCGTACCTAAGAAATTGCGTAAGAAGAGTAAGAAGCATCAGTATGGGACGGAGCCGGAGCCAAATCCAAAGCCAGGTACTGTAGTGAAGTTTGTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

245

Amino Acids

26.72

Weight (kDa)

6.96

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 81
Acc36I ACCTGC 1 cut(s) 81
AciI CCGC 4 cut(s) 59, 122, 140, 539
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 2 cut(s) 3, 81
AfaI GTAC 2 cut(s) 642, 715
AfiI CCNNNNNNNGG 3 cut(s) 90, 614, 710
AgsI TTSAA 6 cut(s) 368, 377, 389, 401, 414, 419
AjnI CCWGG 2 cut(s) 379, 709
AluBI AGCT 3 cut(s) 172, 197, 489
AluI AGCT 3 cut(s) 172, 197, 489
Alw21I GWGCWC 1 cut(s) 164
Alw44I GTGCAC 1 cut(s) 160
AlwI GGATC 1 cut(s) 191
AlwNI CAGNNNCTG 1 cut(s) 716
AoxI GGCC 2 cut(s) 3, 81
ApaLI GTGCAC 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 554
BaeGI GKGCMC 1 cut(s) 164
BaeI ACNNNNGTAYC 2 cut(s) 705, 738
Bbv12I GWGCWC 1 cut(s) 164
BbvCI CCTCAGC 1 cut(s) 72
BccI CCATC 2 cut(s) 347, 607
BceAI ACGGC 1 cut(s) 161
BciT130I CCWGG 2 cut(s) 381, 711
BclI TGATCA 3 cut(s) 16, 466, 544
BfmI CTRYAG 1 cut(s) 717
BfuAI ACCTGC 1 cut(s) 81
Bme1390I CCNGG 2 cut(s) 381, 711
BmiI GGNNCC 2 cut(s) 690, 696
BmrFI CCNGG 2 cut(s) 381, 711
BmsI GCATC 2 cut(s) 46, 682
Bpu10I CCTNAGC 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 380
Bsc4I CCNNNNNNNGG 3 cut(s) 90, 614, 710
Bse118I RCCGGY 2 cut(s) 5, 83
BseBI CCWGG 2 cut(s) 381, 711
BseDI CCNNGG 1 cut(s) 380
BseGI GGATG 2 cut(s) 61, 517
BseLI CCNNNNNNNGG 3 cut(s) 90, 614, 710
BseMII CTCAG 2 cut(s) 63, 600
BseRI GAGGAG 1 cut(s) 337
BseSI GKGCMC 1 cut(s) 164
BseX3I CGGCCG 1 cut(s) 81
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 2 cut(s) 5, 83
BsiEI CGRYCG 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 3 cut(s) 6, 84, 692
BslFI GGGAC 1 cut(s) 697
BslI CCNNNNNNNGG 3 cut(s) 90, 614, 710
BsmFI GGGAC 1 cut(s) 697
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 2 cut(s) 5, 83
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 4 cut(s) 16, 183, 466, 544
BspACI CCGC 4 cut(s) 59, 122, 140, 539
BspANI GGCC 2 cut(s) 5, 83
BspCNI CTCAG 2 cut(s) 64, 601
BspLI GGNNCC 2 cut(s) 690, 696
BspMI ACCTGC 1 cut(s) 81
BspPI GGATC 1 cut(s) 191
BsrFI RCCGGY 2 cut(s) 5, 83
BssAI RCCGGY 2 cut(s) 5, 83
BssECI CCNNGG 1 cut(s) 380
BssMI GATC 4 cut(s) 16, 183, 466, 544
Bst2UI CCWGG 2 cut(s) 381, 711
Bst4CI ACNGT 1 cut(s) 718
Bst6I CTCTTC 3 cut(s) 414, 575, 656
BstC8I GCNNGC 2 cut(s) 7, 174
BstDEI CTNAG 3 cut(s) 72, 609, 645
BstF5I GGATG 2 cut(s) 61, 517
BstKTI GATC 4 cut(s) 19, 186, 469, 547
BstMBI GATC 4 cut(s) 16, 183, 466, 544
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNI CCWGG 2 cut(s) 381, 711
BstSCI CCNGG 2 cut(s) 379, 709
BstSFI CTRYAG 1 cut(s) 717
BstSLI GKGCMC 1 cut(s) 164
BstZI CGGCCG 1 cut(s) 81
BsuRI GGCC 2 cut(s) 5, 83
BtsCI GGATG 2 cut(s) 61, 517
BtsIMutI CAGTG 1 cut(s) 36
BveI ACCTGC 1 cut(s) 81
Cac8I GCNNGC 2 cut(s) 7, 174
CaiI CAGNNNCTG 1 cut(s) 716
Cfr10I RCCGGY 2 cut(s) 5, 83
Csp6I GTAC 2 cut(s) 641, 714
CviAII CATG 1 cut(s) 20
CviQI GTAC 2 cut(s) 641, 714
DdeI CTNAG 3 cut(s) 72, 609, 645
DpnI GATC 4 cut(s) 18, 185, 468, 546
DpnII GATC 4 cut(s) 16, 183, 466, 544
EaeI YGGCCR 2 cut(s) 3, 81
EagI CGGCCG 1 cut(s) 81
Eam1104I CTCTTC 3 cut(s) 414, 575, 656
EarI CTCTTC 3 cut(s) 414, 575, 656
EclXI CGGCCG 1 cut(s) 81
Eco52I CGGCCG 1 cut(s) 81
EcoRII CCWGG 2 cut(s) 379, 709
FaeI CATG 1 cut(s) 23
FaqI GGGAC 1 cut(s) 697
FatI CATG 1 cut(s) 19
FauI CCCGC 1 cut(s) 532
FauNDI CATATG 1 cut(s) 483
FbaI TGATCA 3 cut(s) 16, 466, 544
FokI GGATG 2 cut(s) 68, 524
HaeIII GGCC 2 cut(s) 5, 83
HapII CCGG 3 cut(s) 6, 84, 692
Hin1II CATG 1 cut(s) 23
HinfI GANTC 5 cut(s) 41, 77, 389, 401, 407
HpaII CCGG 3 cut(s) 6, 84, 692
HphI GGTGA 1 cut(s) 554
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 5 cut(s) 31, 48, 55, 394, 406
Hpy8I GTNNAC 1 cut(s) 162
HpyAV CCTTC 2 cut(s) 124, 218
HpyCH4III ACNGT 1 cut(s) 718
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 4 cut(s) 162, 266, 280, 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 3 cut(s) 72, 609, 645
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 1 cut(s) 23
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
Ksp22I TGATCA 3 cut(s) 16, 466, 544
Kzo9I GATC 4 cut(s) 16, 183, 466, 544
LmnI GCTCC 3 cut(s) 617, 688, 694
LpnPI CCDG 9 cut(s) 19, 76, 97, 366, 393, 621, 696, 705, 723
LweI GCATC 2 cut(s) 46, 682
MaeII ACGT 1 cut(s) 396
MalI GATC 4 cut(s) 18, 185, 468, 546
MboI GATC 4 cut(s) 16, 183, 466, 544
MboII GAAGA 7 cut(s) 23, 402, 431, 538, 541, 592, 673
MfeI CAATTG 1 cut(s) 414
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 4 cut(s) 368, 414, 650, 733
MnlI CCTC 5 cut(s) 67, 315, 421, 430, 529
MroNI GCCGGC 1 cut(s) 5
MseI TTAA 2 cut(s) 245, 441
MslI CAYNNNNRTG 2 cut(s) 552, 678
MspI CCGG 3 cut(s) 6, 84, 692
MspR9I CCNGG 2 cut(s) 381, 711
MunI CAATTG 1 cut(s) 414
Mva1269I GAATGC 1 cut(s) 268
MvaI CCWGG 2 cut(s) 381, 711
MwoI GCNNNNNNNGC 1 cut(s) 272
NaeI GCCGGC 1 cut(s) 7
NdeI CATATG 1 cut(s) 483
NdeII GATC 4 cut(s) 16, 183, 466, 544
NgoMIV GCCGGC 1 cut(s) 5
NlaIII CATG 1 cut(s) 23
NlaIV GGNNCC 2 cut(s) 690, 696
PaqCI CACCTGC 1 cut(s) 81
PctI GAATGC 1 cut(s) 268
PdiI GCCGGC 1 cut(s) 7
PfeI GAWTC 5 cut(s) 41, 77, 389, 401, 407
Psp6I CCWGG 2 cut(s) 379, 709
PspGI CCWGG 2 cut(s) 379, 709
PspN4I GGNNCC 2 cut(s) 690, 696
PstNI CAGNNNCTG 1 cut(s) 716
RsaI GTAC 2 cut(s) 642, 715
RsaNI GTAC 2 cut(s) 641, 714
RseI CAYNNNNRTG 2 cut(s) 552, 678
SaqAI TTAA 2 cut(s) 245, 441
Sau3AI GATC 4 cut(s) 16, 183, 466, 544
ScrFI CCNGG 2 cut(s) 381, 711
SduI GDGCHC 1 cut(s) 164
SfaNI GCATC 2 cut(s) 46, 682
SfcI CTRYAG 1 cut(s) 717
SmiMI CAYNNNNRTG 2 cut(s) 552, 678
Sse9I AATT 4 cut(s) 368, 414, 650, 733
SsiI CCGC 4 cut(s) 59, 122, 140, 539
SspI AATATT 1 cut(s) 312
StyD4I CCNGG 2 cut(s) 379, 709
TaaI ACNGT 1 cut(s) 718
TaiI ACGT 1 cut(s) 399
TaqI TCGA 1 cut(s) 260
TasI AATT 4 cut(s) 368, 414, 650, 733
TfiI GAWTC 5 cut(s) 41, 77, 389, 401, 407
Tru1I TTAA 2 cut(s) 245, 441
Tru9I TTAA 2 cut(s) 245, 441
TscAI CASTG 1 cut(s) 43
TspDTI ATGAA 1 cut(s) 263
TspGWI ACGGA 1 cut(s) 701
TspRI CASTG 1 cut(s) 43
VneI GTGCAC 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.