Rorug05G0035100

Ethylene-responsive nuclear protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002810
Physical Location & Seq
Forward (+)
2442544 .. 2443893
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0035100.1

Sequence Viewer

Length: 705 bp
ATGGCACAAGCTATGGCTTCAATGGCTGGTCTACACGGTTCCTCCCAGGCTGTGTTGGAAGGCAGCAGCCTCCATCTAAGCGGCTCATCTCGCTTGAACATAGTTGGTAACAGCACCAGGCTAGCCGTGACAAGGCCCGGCTTTGCCATTAGAGCTCAGCAAGTGCCCGCTGAGCCTGAGACTAGCCGTCGCGCTATGCTCGGTCTTGTGGCAGTTGGTTTGGCCTCGGGATCTTTCGTTCAGGCTGTGCTTGCTGAGGCTAAGCCCATCAAAATCGGCCCTCCTCCCCCCCCATCCGGTGGATTGCCTGGAACTGAGAACTCGGATGAGGCAAGAGACCTTGATCTGCCATTGCAGAAGAGGTTCTTCTTGCAGCCACTGACTCCAGCTCAGGCTGCCGCGAGGGCAAAGGAGTCAGCCAAGGAAATCCTTGCCGTCAAGTCGTTCATAGACAAGAAGGCATGGCCATTTGTCCAGAACGATCTTCGTCTCAGGGCTTCATATCTCCGATATGACCTCAAAACAGTCCTCGCAGCTGCCAAGTCCAAGGAAGAGAAGGAGTCATTGAAGACACTCACCGGAAAGCTCTTCCAAGACCTTGACAATTTGGACTATGCAGCAAAGATCAAAAGCACCCCACAAGCGGAGAAGTACTATGCCGAAGCTGTATCTAGCCTTAATAATGTTGTTGCAAAGCTTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

234

Amino Acids

24.97

Weight (kDa)

9.64

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbQ PF05757 1 - 234 7e-73 Oxygen evolving enhancer protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 299
AccI GTMKAC 1 cut(s) 31
AccII CGCG 2 cut(s) 192, 401
AciI CCGC 4 cut(s) 81, 168, 399, 644
AclWI GGATC 1 cut(s) 238
AcoI YGGCCR 1 cut(s) 464
AfaI GTAC 1 cut(s) 653
AfiI CCNNNNNNNGG 4 cut(s) 132, 296, 299, 643
AgsI TTSAA 3 cut(s) 21, 97, 568
AhdI GACNNNNNGTC 1 cut(s) 186
AjnI CCWGG 3 cut(s) 45, 116, 307
AloI GAACNNNNNNTCC 2 cut(s) 222, 254
AluBI AGCT 7 cut(s) 11, 155, 389, 536, 586, 665, 697
AluI AGCT 7 cut(s) 11, 155, 389, 536, 586, 665, 697
Alw21I GWGCWC 1 cut(s) 157
Alw26I GTCTC 3 cut(s) 173, 330, 494
AlwI GGATC 1 cut(s) 238
AlwNI CAGNNNCTG 1 cut(s) 379
Ama87I CYCGRG 1 cut(s) 226
AoxI GGCC 4 cut(s) 134, 222, 277, 464
ApeKI GCWGC 7 cut(s) 63, 66, 373, 395, 533, 536, 617
Asp700I GAANNNNTTC 1 cut(s) 362
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 2 cut(s) 135, 278
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 1 cut(s) 568
AsuNHI GCTAGC 1 cut(s) 121
AvaI CYCGRG 1 cut(s) 226
BaeGI GKGCMC 1 cut(s) 168
BalI TGGCCA 1 cut(s) 466
BanII GRGCYC 1 cut(s) 157
BbsI GAAGAC 1 cut(s) 575
Bbv12I GWGCWC 1 cut(s) 157
BbvCI CCTCAGC 1 cut(s) 255
BbvI GCAGC 7 cut(s) 75, 78, 382, 385, 523, 545, 629
BccI CCATC 3 cut(s) 81, 275, 301
BceAI ACGGC 3 cut(s) 110, 171, 419
BciT130I CCWGG 3 cut(s) 47, 118, 309
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 3 cut(s) 173, 330, 494
BfaI CTAG 3 cut(s) 122, 183, 672
BglI GCCNNNNNGGC 1 cut(s) 404
BisI GCNGC 9 cut(s) 64, 67, 82, 374, 396, 399, 534, 537, 618
BlpI GCTNAGC 3 cut(s) 156, 171, 261
BlsI GCNGC 9 cut(s) 65, 68, 83, 375, 397, 400, 535, 538, 619
BmcAI AGTACT 1 cut(s) 653
Bme1390I CCNGG 4 cut(s) 47, 118, 138, 309
BmeRI GACNNNNNGTC 1 cut(s) 186
BmeT110I CYCGRG 1 cut(s) 226
BmgT120I GGNCC 2 cut(s) 135, 278
BmiI GGNNCC 1 cut(s) 40
BmrFI CCNGG 4 cut(s) 47, 118, 138, 309
BmtI GCTAGC 1 cut(s) 125
BpiI GAAGAC 1 cut(s) 575
BpmI CTGGAG 1 cut(s) 369
Bpu10I CCTNAGC 2 cut(s) 255, 390
Bpu1102I GCTNAGC 3 cut(s) 156, 171, 261
BpuMI CCSGG 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 330
BsaJI CCNNGG 4 cut(s) 45, 225, 420, 546
BsaWI WCCGGW 2 cut(s) 296, 578
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 296, 299, 643
Bse3DI GCAATG 1 cut(s) 350
BseBI CCWGG 3 cut(s) 47, 118, 309
BseDI CCNNGG 4 cut(s) 45, 225, 420, 546
BseGI GGATG 2 cut(s) 293, 331
BseLI CCNNNNNNNGG 4 cut(s) 132, 296, 299, 643
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 7 cut(s) 162, 168, 170, 246, 306, 404, 505
BseRI GAGGAG 1 cut(s) 273
BseSI GKGCMC 1 cut(s) 168
BseXI GCAGC 7 cut(s) 75, 78, 382, 385, 523, 545, 629
Bsh1236I CGCG 2 cut(s) 192, 401
BshFI GGCC 4 cut(s) 136, 224, 279, 466
BsiHKAI GWGCWC 1 cut(s) 157
BsiHKCI CYCGRG 1 cut(s) 226
BsiSI CCGG 3 cut(s) 138, 297, 579
BslI CCNNNNNNNGG 4 cut(s) 132, 296, 299, 643
BsmAI GTCTC 3 cut(s) 173, 330, 494
BsmBI CGTCTC 1 cut(s) 494
BsnI GGCC 4 cut(s) 136, 224, 279, 466
Bso31I GGTCTC 1 cut(s) 330
BsoBI CYCGRG 1 cut(s) 226
Bsp1286I GDGCHC 2 cut(s) 157, 168
Bsp143I GATC 4 cut(s) 230, 343, 481, 624
Bsp1720I GCTNAGC 3 cut(s) 156, 171, 261
BspACI CCGC 4 cut(s) 81, 168, 399, 644
BspANI GGCC 4 cut(s) 136, 224, 279, 466
BspCNI CTCAG 7 cut(s) 163, 169, 169, 247, 307, 403, 504
BspFNI CGCG 2 cut(s) 192, 401
BspLI GGNNCC 1 cut(s) 40
BspOI GCTAGC 1 cut(s) 125
BspPI GGATC 1 cut(s) 238
BspQI GCTCTTC 1 cut(s) 593
BspTNI GGTCTC 1 cut(s) 330
BsrDI GCAATG 1 cut(s) 350
BssECI CCNNGG 4 cut(s) 45, 225, 420, 546
BssMI GATC 4 cut(s) 230, 343, 481, 624
BssT1I CCWWGG 2 cut(s) 420, 546
Bst2UI CCWGG 3 cut(s) 47, 118, 309
Bst4CI ACNGT 2 cut(s) 38, 526
Bst6I CTCTTC 3 cut(s) 353, 546, 593
BstC8I GCNNGC 3 cut(s) 123, 168, 252
BstDEI CTNAG 9 cut(s) 77, 156, 171, 177, 255, 261, 315, 390, 491
BstF5I GGATG 2 cut(s) 293, 331
BstFNI CGCG 2 cut(s) 192, 401
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 4 cut(s) 233, 346, 484, 627
BstMAI GTCTC 3 cut(s) 173, 330, 494
BstMBI GATC 4 cut(s) 230, 343, 481, 624
BstMWI GCNNNNNNNGC 7 cut(s) 23, 90, 152, 172, 251, 395, 404
BstNI CCWGG 3 cut(s) 47, 118, 309
BstSCI CCNGG 4 cut(s) 45, 116, 136, 307
BstSLI GKGCMC 1 cut(s) 168
BstUI CGCG 2 cut(s) 192, 401
BstV1I GCAGC 7 cut(s) 75, 78, 382, 385, 523, 545, 629
BstV2I GAAGAC 1 cut(s) 575
BstX2I RGATCY 1 cut(s) 230
BstYI RGATCY 1 cut(s) 230
BsuRI GGCC 4 cut(s) 136, 224, 279, 466
BtsCI GGATG 2 cut(s) 293, 331
BtsIMutI CAGTG 1 cut(s) 377
Cac8I GCNNGC 3 cut(s) 123, 168, 252
CaiI CAGNNNCTG 1 cut(s) 379
CfoI GCGC 1 cut(s) 194
Cfr13I GGNCC 2 cut(s) 135, 278
Csp6I GTAC 1 cut(s) 652
CviAII CATG 1 cut(s) 462
CviQI GTAC 1 cut(s) 652
DdeI CTNAG 9 cut(s) 77, 156, 171, 177, 255, 261, 315, 390, 491
DpnI GATC 4 cut(s) 232, 345, 483, 626
DpnII GATC 4 cut(s) 230, 343, 481, 624
DriI GACNNNNNGTC 1 cut(s) 186
EaeI YGGCCR 1 cut(s) 464
Eam1104I CTCTTC 3 cut(s) 353, 546, 593
Eam1105I GACNNNNNGTC 1 cut(s) 186
EarI CTCTTC 3 cut(s) 353, 546, 593
Ecl136II GAGCTC 1 cut(s) 155
Eco130I CCWWGG 2 cut(s) 420, 546
Eco24I GRGCYC 1 cut(s) 157
Eco31I GGTCTC 1 cut(s) 330
Eco53kI GAGCTC 1 cut(s) 155
Eco88I CYCGRG 1 cut(s) 226
EcoICRI GAGCTC 1 cut(s) 155
EcoRII CCWGG 3 cut(s) 45, 116, 307
EcoT14I CCWWGG 2 cut(s) 420, 546
EcoT38I GRGCYC 1 cut(s) 157
ErhI CCWWGG 2 cut(s) 420, 546
Esp3I CGTCTC 1 cut(s) 494
FaeI CATG 1 cut(s) 465
FaiI YATR 9 cut(s) 14, 101, 197, 449, 463, 502, 513, 615, 657
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FatI CATG 1 cut(s) 461
FauI CCCGC 1 cut(s) 175
FblI GTMKAC 1 cut(s) 31
Fnu4HI GCNGC 9 cut(s) 64, 67, 82, 374, 396, 399, 534, 537, 618
FokI GGATG 2 cut(s) 280, 338
FriOI GRGCYC 1 cut(s) 157
Fsp4HI GCNGC 9 cut(s) 64, 67, 82, 374, 396, 399, 534, 537, 618
FspBI CTAG 3 cut(s) 122, 183, 672
GlaI GCGC 1 cut(s) 193
GluI GCNGC 9 cut(s) 64, 67, 82, 374, 396, 399, 534, 537, 618
GsuI CTGGAG 1 cut(s) 369
HaeIII GGCC 4 cut(s) 136, 224, 279, 466
HapII CCGG 3 cut(s) 138, 297, 579
HhaI GCGC 1 cut(s) 194
Hin1II CATG 1 cut(s) 465
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HindIII AAGCTT 1 cut(s) 695
HinfI GANTC 3 cut(s) 382, 413, 560
HpaII CCGG 3 cut(s) 138, 297, 579
HphI GGTGA 1 cut(s) 568
Hpy166II GTNNAC 1 cut(s) 32
Hpy188I TCNGA 2 cut(s) 325, 509
Hpy188III TCNNGA 2 cut(s) 228, 475
Hpy8I GTNNAC 1 cut(s) 32
Hpy99I CGWCG 1 cut(s) 192
HpyAV CCTTC 3 cut(s) 53, 451, 550
HpyCH4III ACNGT 2 cut(s) 38, 526
HpyCH4V TGCA 4 cut(s) 355, 373, 617, 692
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 90, 152, 172, 251, 395, 404
HpyF3I CTNAG 9 cut(s) 77, 156, 171, 177, 255, 261, 315, 390, 491
Hsp92II CATG 1 cut(s) 465
HspAI GCGC 1 cut(s) 192
Kzo9I GATC 4 cut(s) 230, 343, 481, 624
LguI GCTCTTC 1 cut(s) 593
Lsp1109I GCAGC 7 cut(s) 75, 78, 382, 385, 523, 545, 629
MaeI CTAG 3 cut(s) 122, 183, 672
MaeIII GTNAC 2 cut(s) 107, 127
MalI GATC 4 cut(s) 232, 345, 483, 626
MboI GATC 4 cut(s) 230, 343, 481, 624
MboII GAAGA 6 cut(s) 358, 370, 476, 563, 580, 580
MflI RGATCY 1 cut(s) 230
MhlI GDGCHC 2 cut(s) 157, 168
MlsI TGGCCA 1 cut(s) 466
MluCI AATT 1 cut(s) 604
MluNI TGGCCA 1 cut(s) 466
MlyI GAGTC 3 cut(s) 376, 422, 569
MmeI TCCRAC 1 cut(s) 36
Mox20I TGGCCA 1 cut(s) 466
MroXI GAANNNNTTC 1 cut(s) 362
MscI TGGCCA 1 cut(s) 466
MseI TTAA 2 cut(s) 678, 703
Msp20I TGGCCA 1 cut(s) 466
MspA1I CMGCKG 2 cut(s) 170, 536
MspI CCGG 3 cut(s) 138, 297, 579
MspR9I CCNGG 4 cut(s) 47, 118, 138, 309
MvaI CCWGG 3 cut(s) 47, 118, 309
MvnI CGCG 2 cut(s) 192, 401
MwoI GCNNNNNNNGC 7 cut(s) 23, 90, 152, 172, 251, 395, 404
NciI CCSGG 1 cut(s) 138
NdeII GATC 4 cut(s) 230, 343, 481, 624
NheI GCTAGC 1 cut(s) 121
NlaIII CATG 1 cut(s) 465
NlaIV GGNNCC 1 cut(s) 40
NmuCI GTSAC 1 cut(s) 127
PciSI GCTCTTC 1 cut(s) 593
PdmI GAANNNNTTC 1 cut(s) 362
PflMI CCANNNNNTGG 1 cut(s) 299
PkrI GCNGC 9 cut(s) 65, 68, 83, 375, 397, 400, 535, 538, 619
PleI GAGTC 3 cut(s) 376, 421, 568
PpsI GAGTC 3 cut(s) 376, 421, 568
Psp124BI GAGCTC 1 cut(s) 157
Psp6I CCWGG 3 cut(s) 45, 116, 307
PspGI CCWGG 3 cut(s) 45, 116, 307
PspN4I GGNNCC 1 cut(s) 40
PspPI GGNCC 2 cut(s) 135, 278
PstNI CAGNNNCTG 1 cut(s) 379
PsuI RGATCY 1 cut(s) 230
PvuII CAGCTG 1 cut(s) 536
RsaI GTAC 1 cut(s) 653
RsaNI GTAC 1 cut(s) 652
SacI GAGCTC 1 cut(s) 157
SapI GCTCTTC 1 cut(s) 593
SaqAI TTAA 2 cut(s) 678, 703
SatI GCNGC 9 cut(s) 64, 67, 82, 374, 396, 399, 534, 537, 618
Sau3AI GATC 4 cut(s) 230, 343, 481, 624
Sau96I GGNCC 2 cut(s) 135, 278
ScaI AGTACT 1 cut(s) 653
SchI GAGTC 3 cut(s) 376, 422, 569
ScrFI CCNGG 4 cut(s) 47, 118, 138, 309
SduI GDGCHC 2 cut(s) 157, 168
Sse9I AATT 1 cut(s) 604
SsiI CCGC 4 cut(s) 81, 168, 399, 644
SspMI CTAG 3 cut(s) 122, 183, 672
SstI GAGCTC 1 cut(s) 157
StyD4I CCNGG 4 cut(s) 45, 116, 136, 307
StyI CCWWGG 2 cut(s) 420, 546
TaaI ACNGT 2 cut(s) 38, 526
TaqII GACCGA 1 cut(s) 191
TasI AATT 1 cut(s) 604
TatI WGTACW 1 cut(s) 651
TauI GCSGC 2 cut(s) 84, 401
Tru1I TTAA 2 cut(s) 678, 703
Tru9I TTAA 2 cut(s) 678, 703
TscAI CASTG 1 cut(s) 384
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 7 cut(s) 63, 66, 373, 395, 533, 536, 617
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 2 cut(s) 436, 489
TspRI CASTG 1 cut(s) 384
Van91I CCANNNNNTGG 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 31
XmnI GAANNNNTTC 1 cut(s) 362
XspI CTAG 3 cut(s) 122, 183, 672
ZrmI AGTACT 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.