Rroxscaffold_2G00110770

AtCEST,CEST

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003520
Physical Location & Seq
Forward (+)
35241523 .. 35256502
14980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00110770.1

Sequence Viewer

Length: 339 bp
ATGTCACCAAATCTTGCCTCTATTCAATTACCCAGTTTGAATACAGCTGCTGGTACCATGTCTCCCTTTGAACATGGCGAGTTTTTTGTTCTTGATGATGGTGGAGAGCTCACCATTGGGATTGGGGACACAAGAGAAGTTGAGCGCAAGGAACTCCTAGGCATCGAAGACCCCGATGCTCCCACCAGGGAAGAGTTAGTTGTTGTTCTTGAAGAAGTCGGGAGAATCGGGGCAGAGATCGAGTGGATTAGAGTATTATTTTGGTCTTACAGTATTTGTAAATGGGGAGGAAATTATATATTGAGTGAGGTGTTGAGTTTATTGTTTGAGAAACAATAA

Protein Analysis

112

Amino Acids

12.48

Weight (kDa)

4.27

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTP_synth_N PF06418 11 - 43 2.5e-08 CTP synthase N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 53
AccB1I GGYRCC 1 cut(s) 53
AfaI GTAC 1 cut(s) 55
AgsI TTSAA 4 cut(s) 26, 40, 71, 212
AjnI CCWGG 1 cut(s) 185
AluBI AGCT 2 cut(s) 47, 109
AluI AGCT 2 cut(s) 47, 109
Alw21I GWGCWC 1 cut(s) 111
Alw26I GTCTC 1 cut(s) 66
AlwNI CAGNNNCTG 1 cut(s) 50
ApeKI GCWGC 1 cut(s) 47
Asp718I GGTACC 1 cut(s) 53
AspA2I CCTAGG 1 cut(s) 157
AspLEI GCGC 1 cut(s) 147
AsuHPI GGTGA 1 cut(s) 103
AvrII CCTAGG 1 cut(s) 157
BanI GGYRCC 1 cut(s) 53
BanII GRGCYC 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 111
BbvI GCAGC 1 cut(s) 34
BccI CCATC 1 cut(s) 92
BciT130I CCWGG 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 66
BfaI CTAG 1 cut(s) 158
BisI GCNGC 1 cut(s) 48
BlnI CCTAGG 1 cut(s) 157
BlsI GCNGC 1 cut(s) 49
Bme1390I CCNGG 1 cut(s) 187
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 187
BmrI ACTGGG 1 cut(s) 27
BmsI GCATC 2 cut(s) 166, 171
BmuI ACTGGG 1 cut(s) 27
BpiI GAAGAC 1 cut(s) 174
BsaJI CCNNGG 2 cut(s) 157, 186
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bse1I ACTGG 1 cut(s) 33
BseBI CCWGG 1 cut(s) 187
BseDI CCNNGG 2 cut(s) 157, 186
BseNI ACTGG 1 cut(s) 33
BseXI GCAGC 1 cut(s) 34
BshNI GGYRCC 1 cut(s) 53
BsiHKAI GWGCWC 1 cut(s) 111
BslFI GGGAC 1 cut(s) 140
BsmAI GTCTC 1 cut(s) 66
BsmFI GGGAC 1 cut(s) 140
Bsp1286I GDGCHC 1 cut(s) 111
Bsp143I GATC 1 cut(s) 237
BspLI GGNNCC 1 cut(s) 55
BspT107I GGYRCC 1 cut(s) 53
BsrI ACTGG 1 cut(s) 33
BssECI CCNNGG 2 cut(s) 157, 186
BssMI GATC 1 cut(s) 237
BssT1I CCWWGG 1 cut(s) 157
Bst2UI CCWGG 1 cut(s) 187
Bst4CI ACNGT 1 cut(s) 272
Bst6I CTCTTC 1 cut(s) 186
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 1 cut(s) 240
BstMAI GTCTC 1 cut(s) 66
BstMBI GATC 1 cut(s) 237
BstNI CCWGG 1 cut(s) 187
BstSCI CCNGG 1 cut(s) 185
BstV1I GCAGC 1 cut(s) 34
BstV2I GAAGAC 1 cut(s) 174
CaiI CAGNNNCTG 1 cut(s) 50
CfoI GCGC 1 cut(s) 147
Csp6I GTAC 1 cut(s) 54
CviAII CATG 2 cut(s) 58, 74
CviJI RGCY 2 cut(s) 47, 109
CviKI_1 RGCY 2 cut(s) 47, 109
CviQI GTAC 1 cut(s) 54
DpnI GATC 1 cut(s) 239
DpnII GATC 1 cut(s) 237
Eam1104I CTCTTC 1 cut(s) 186
EarI CTCTTC 1 cut(s) 186
Ecl136II GAGCTC 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 157
Eco24I GRGCYC 1 cut(s) 111
Eco53kI GAGCTC 1 cut(s) 109
EcoICRI GAGCTC 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 185
EcoT14I CCWWGG 1 cut(s) 157
EcoT38I GRGCYC 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 157
FaeI CATG 2 cut(s) 61, 77
FaiI YATR 4 cut(s) 59, 75, 297, 299
FaqI GGGAC 1 cut(s) 140
FatI CATG 2 cut(s) 57, 73
Fnu4HI GCNGC 1 cut(s) 48
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 1 cut(s) 48
FspBI CTAG 1 cut(s) 158
GlaI GCGC 1 cut(s) 146
GluI GCNGC 1 cut(s) 48
HhaI GCGC 1 cut(s) 147
Hin1II CATG 2 cut(s) 61, 77
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HinfI GANTC 1 cut(s) 225
HphI GGTGA 1 cut(s) 103
Hpy188III TCNNGA 3 cut(s) 92, 209, 220
HpyCH4III ACNGT 1 cut(s) 272
Hsp92II CATG 2 cut(s) 61, 77
HspAI GCGC 1 cut(s) 145
KpnI GGTACC 1 cut(s) 57
Kzo9I GATC 1 cut(s) 237
LmnI GCTCC 1 cut(s) 184
LpnPI CCDG 4 cut(s) 36, 46, 172, 199
Lsp1109I GCAGC 1 cut(s) 34
LweI GCATC 2 cut(s) 166, 171
MaeI CTAG 1 cut(s) 158
MaeIII GTNAC 1 cut(s) 3
MalI GATC 1 cut(s) 239
MboI GATC 1 cut(s) 237
MboII GAAGA 3 cut(s) 179, 203, 224
MhlI GDGCHC 1 cut(s) 111
MluCI AATT 2 cut(s) 26, 292
MnlI CCTC 3 cut(s) 28, 281, 301
MspA1I CMGCKG 1 cut(s) 47
MspR9I CCNGG 1 cut(s) 187
MvaI CCWGG 1 cut(s) 187
NdeII GATC 1 cut(s) 237
NlaIII CATG 2 cut(s) 61, 77
NlaIV GGNNCC 1 cut(s) 55
NmuCI GTSAC 1 cut(s) 3
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PfeI GAWTC 1 cut(s) 225
PkrI GCNGC 1 cut(s) 49
Psp124BI GAGCTC 1 cut(s) 111
Psp6I CCWGG 1 cut(s) 185
PspGI CCWGG 1 cut(s) 185
PspN4I GGNNCC 1 cut(s) 55
PstNI CAGNNNCTG 1 cut(s) 50
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
SacI GAGCTC 1 cut(s) 111
SatI GCNGC 1 cut(s) 48
Sau3AI GATC 1 cut(s) 237
ScrFI CCNGG 1 cut(s) 187
SduI GDGCHC 1 cut(s) 111
SetI ASST 3 cut(s) 49, 111, 312
SfaNI GCATC 2 cut(s) 166, 171
Sse9I AATT 2 cut(s) 26, 292
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 111
StyD4I CCNGG 1 cut(s) 185
StyI CCWWGG 1 cut(s) 157
TaaI ACNGT 1 cut(s) 272
TaqI TCGA 2 cut(s) 165, 240
TasI AATT 2 cut(s) 26, 292
TfiI GAWTC 1 cut(s) 225
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 47
Tsp45I GTSAC 1 cut(s) 3
XmaJI CCTAGG 1 cut(s) 157
XspI CTAG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.