Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0002490

Physical Location & Seq

Genomic Coordinates Reverse (-)

66230068 .. 66236772

Gene Structure 6705 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00274620.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3132 bp

ATGTCTCCTCTCGCCGAGAACGACGAGCTTTCTTACTACGACGACGACGACGATGACAACGACGGCTTCAACGCCGACATCGAGGCCCTCCGCCGCGCCTGTATGCTCACCGGCAAAGACCCCGACGACATCACCACCAAAAACGACGACGAAAACCCTAGCAGCAACCAAAATGACGACGTAGACCTCTCCTCGGCTTCCAATTCCGACTCCGATTTCGACGAGGACCTCGAGCTGCTCCGCAAAATCAAGAGCCAATTCTGCAACACTTCTATCAAGCCTCTTTCGCTGAAGCCTCTCTGTACTTTGCTTCCGCCTTCTACCTCCGACGAGGAGGAGGACGACTACCAGACGCTGCTCGCCGTTCGGAAACGTTTCGCCGCCTACGAGAACGATACTCAGGAAAAAAGTGCAGAGCAGATTGATCCTTCCTGCAAATCAGTCGAGGAAACTTGCAATGAAGTATCTGGAACTGATGCTCATGAAAGGTTTCTTACTCTGGTCACCGATAATGAAGAGGGACAGCCTTCCACTTCCATTGAGTGGGTTCAGTCAGATTCTTGTGAGCCAACTCTGTTGCCAACCAAAAGTTCAGGGTTCCCAAAGTCTGCACTGGCTTTTCTGGATGCTATTAATAAGAATAGGTCCTGTCAGAAGTTTCTTCGAAGTAAGCTAATACAAATCCAAGCGAAAATTGAGGAGAACAACAAACTGAAGCAGCGTGTCAAAATCCTTAGAGACTTTCAAGCTCATTGCAAAAGACGAACAGGAGAGGCGCTGTCCCAAAAGAAGGATCCTCGTGTCCAGTTAATTTTAACGAAAAAGCCAAGGGCTGATTCAAAGGCTCATGACAGAGGTATCTCAGCTATGTCTTATGGCCCAGAAGAAAATTCACATGTTGCCAACTACAGAAAGGCATCGACAAAATTTCCACTTTCATTAGATCGAAAAAATTGGTCAGAGGTAGAAAAGAAAGATCTTCAAAATGGAATAAGACAACAATTTCAGGAAATGGTGCTTCAATCTTCCGTAGATGATTCAAGTTATTCTGAGACACCTCATGGAGACTCGAATGACATTGATAAAATTTTGGCTTCAATCAAGGATCTCGAAATTACCCCAAATAGCATTAGAGAGTTCCTGCCTAAGGTTAACTGGGAGCAGTTGGCTTCCATGTATGTTCCTGGTCGCTCTGGTGGAGAATGTGAAGCAAGGTGGTTGAACTGGGAGGATCCCTTAATCAATCATGGACCATGGACAACAGATGAGGATAAGATGATTCTGTATCTTGTCCAGAAGAAGAATGGGGTCAATGATTGGAATAATATTGCAGAAGCAGTGGGGACCAACAGGACTCCATTTCAGTGCTTGGCACGCTACCAAAGGAGCCTAAATGCTTCTATACTGAAACGGGAGTGGACCAAGGATGAGGATGCAAAACTTGCTTCTGCTGTAGAAGCTTTTGGTGAGGGTAATTGGCAGTCTGTAGCTTCTGCTTTGAACGGACGAACTGGTACTCAATGCTCTAATAGATGGAAGAAATCACTTCATCCAACCAGGACAAGAGCAGGTAAGTGGACTCCAGAAGAAGACAAACGCCTGATAGTATCTCAAATGCTTTTTGAATCAAAAAGTTGGAATAAGACAGCTCAGTTTGTGCCAGGCCGAACTCAGGCACAATGCAGAGATAGATATGTCAACTCTTTAGAACCTTCTTTGAGGTGTGGTGAATGGACTGAAGAAGAGGACTCAAGGTTGATAGCAGCAATTGAAGAATATGGACATTGCTGGTCAAAAGTTGCTGCACGTGTGCCTCACCGGACTGATAATATGTGCTGGAGGAGATGGAAGGTGTTGTTTCCGGATGAATTGCCCTTGCTCAGAGAAGAGAAAAGAATACGTAAGACTGCTCTCATCCGCAACTTTGTTGATCGGGAGAAAGAACGTCCAGCCCTTGGCCCTAATGATTTTCTTCTACAAGATGTTACCTCAATGAAAACTTTAACTTATCCTAGGAAGACGGGAGGATTTAGCCAAGTTCCAAAGAAGATTAAACCCAGGAGGCATAGAAACGATGCTCAGCTTTGCTCTGAGGAAGAACCTGGGACAAGCAATTCTGAGGTTGAGACTTATGATGGGGAAGATGTGTCAAAGAAGAAGAAGAAGAAGAAAGTTCTTAAACAGCGTCAAAGAAGGCACAAAGACAATGAACCCACAGGAGACTGTGAGGCTGTTGTACCTGTACCAAAGAAGATTAGACCCAGGAGGAATAGAAACAACGGTCAAATTCGTGGGACAAGCAATTCTGAGGTTCGGACATGTGATGGGGATGATGGCCCGAAGAAGAAGAAAGTTCTTAAACAGCGTCACAGAAGGCACAAAGGCACTGAACTAACAGGAGAGTGTAAGGCTATTGTACCTGTTAGTAATGAGGATGACACACACAAAGAACCCACAGAAATGTCTTGTTCAGATGCCCTGAAGAAGAAGAAAGTTCTTAAACATCGTCACAGAAAGCACAAAGGCACTGAACCAACAGGAGAGTGTAAGGCTATTGTACCTGTTAGTAACGAGGATGACGCACGCAAAGAACCCACAGCAATGTCTTATTCAGATCAAATTATAGGAACATCAGATGAAGATGATACTGTTTCTTACTATCTACATAAATCAAGGAAGAGAAAGCTTGAAGCTGATCTGGCTGTCCAGGAGTCCCCCTTCTACTGTATAAGGGATCGTGAAGCTTTAACACAACATGAGCCAGCCATAGCTTGCAATAAACCTGGTCGACGAAAATTAGTCAGAGGGCAAAAGCTTGGCCTCCAAGAACCAATGAGGGGAGTGTTGAGAATTTGTCCAGGAAGTGATCATGAGACTGATGATCCTGTTGTGGCTTCCACGGGGAAAAAGAATTCAAAGAAGAGGCATGCTCAAACTACCAAACGGCATGTCATTTGTAATGATCATGATGCAGGAGATCTACCTAAACAAGAGGAGGTCGCAATCAAATCTGGGAAGGAACAAGTGAGCGCTGGTACAGACAGCGGCGATGATATAACACTAGCTTGTTTTCTTAATAGATTAAAGAAGCGATGTGAATCTGGCAGAGGAGATATTCAGATGCCGAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0022402 GO:0022607 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044085 GO:0044212 GO:0044424 GO:0044464 GO:0048285 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051225 GO:0051252 GO:0060255 GO:0065007 GO:0070925 GO:0071840 GO:0080090 GO:0090307 GO:0097159 GO:0140014 GO:0140110 GO:1901363 GO:1902850 GO:1903047 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

1043

Amino Acids

118.58

Weight (kDa)

8.26

Isoelectric Point (pI)

51.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	417 - 462	9.6e-10	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	419 - 478	2.3e-13	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	470 - 514	6.9e-14	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	473 - 522	2.1e-11	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	524 - 567	3.2e-09	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	526 - 581	3.9e-10	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	576 - 617	1e-13	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	578 - 621	3.1e-07	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0002490)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18100 AT3G18100 AT3G18100 AT3G18100
fragaria_vesca	FvH4_5g17070 FvH4_5g17070 FvH4_5g17070 FvH4_5g17070 FvH4_5g17070 FvH4_5g17070 FvH4_5g17070 FvH4_5g17070
malus_domestica	MD14G1233900.v1.1
prunus_persica	Prupe.5G230100_v2.0.a1 Prupe.5G230100_v2.0.a1
pyrus_communis	pycom14g19490
rosa_chinensis	RchiOBHm_Chr7g0178781 RchiOBHm_Chr7g0230201
rosa_laevigata	RLG00000005416
rosa_multiflora	Rmu_sc0001901.1_g000001 Rmu_sc0002822.1_g000027 Rmu_sc0004110.1_g000007 Rmu_sc0004110.1_g000008 Rmu_sc0015029.1_g000002 Rmu_sc0025325.1_g000009
rosa_roxburghii	Rroxscaffold_3G00230300 Rroxscaffold_3G00274620
rosa_rugosa	Rorug06G0419400 Rorug06G0419500 Rorug06G0419600
rosa_samantha	Rh7AG018900 Rh7AG408900 Rh7AG409000 Rh7BG018200 Rh7BG388800 Rh7CG019000 Rh7DG018800 Rh7DG406800
rosa_wichuraiana	Rw0G022580 Rw0G022590 Rw7G001530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1559
AccB7I	CCANNNNNTGG	2 cut(s)	543, 1955
AccI	GTMKAC	2 cut(s)	183, 2788
AccII	CGCG	1 cut(s)	96
AccIII	TCCGGA	1 cut(s)	1861
AciI	CCGC	7 cut(s)	91, 94, 241, 314, 381, 1918, 3045
AclI	AACGTT	1 cut(s)	373
AclWI	GGATC	8 cut(s)	419, 788, 801, 1113, 1226, 1239, 2742, 2876
AcsI	RAATTY	6 cut(s)	889, 926, 1086, 2286, 2850, 2911
AcuI	CTGAAG	4 cut(s)	311, 734, 1758, 2501
AcvI	CACGTG	1 cut(s)	1808
AfaI	GTAC	7 cut(s)	304, 1516, 2238, 2244, 2418, 2559, 3037
AfeI	AGCGCT	1 cut(s)	3031
AfiI	CCNNNNNNNGG	7 cut(s)	193, 543, 790, 1147, 1672, 1955, 2837
AflIII	ACRYGT	3 cut(s)	895, 1807, 2318
AjnI	CCWGG	9 cut(s)	1183, 1556, 1660, 2057, 2101, 2261, 2706, 2782, 2857
AjuI	GAANNNNNNNTTGG	4 cut(s)	574, 606, 820, 852
Alw26I	GTCTC	7 cut(s)	9, 732, 1046, 1059, 2120, 2214, 2867
AlwI	GGATC	8 cut(s)	419, 788, 801, 1113, 1226, 1239, 2742, 2876
AlwNI	CAGNNNCTG	1 cut(s)	355
Ama87I	CYCGRG	1 cut(s)	230
Aor13HI	TCCGGA	1 cut(s)	1861
Aor51HI	AGCGCT	1 cut(s)	3031
AoxI	GGCC	6 cut(s)	84, 877, 1663, 1957, 2335, 2818
ApeKI	GCWGC	6 cut(s)	162, 235, 355, 718, 1763, 1802
ApoI	RAATTY	6 cut(s)	889, 926, 1086, 2286, 2850, 2911
ArsI	GACNNNNNNTTYG	4 cut(s)	200, 232, 2934, 2966
AseI	ATTAAT	1 cut(s)	633
Asp700I	GAANNNNTTC	2 cut(s)	374, 489
AspA2I	CCTAGG	1 cut(s)	2012
AspLEI	GCGC	3 cut(s)	98, 778, 3032
AspS9I	GGNCC	9 cut(s)	85, 226, 645, 878, 1250, 1344, 1419, 1958, 2336
AsuHPI	GGTGA	6 cut(s)	100, 124, 496, 1478, 1739, 1808
AsuII	TTCGAA	1 cut(s)	664
AvaI	CYCGRG	1 cut(s)	230
AvaII	GGWCC	5 cut(s)	226, 645, 1250, 1344, 1419
AvrII	CCTAGG	1 cut(s)	2012
AxyI	CCTNAGG	1 cut(s)	1146
BaeI	ACNNNNGTAYC	2 cut(s)	841, 874
BamHI	GGATCC	2 cut(s)	793, 1231
BauI	CACGAG	1 cut(s)	798
BbrPI	CACGTG	1 cut(s)	1808
BbsI	GAAGAC	2 cut(s)	1596, 2024
BbvI	GCAGC	6 cut(s)	174, 222, 342, 730, 1775, 1789
BccI	CCATC	5 cut(s)	1527, 1839, 2129, 2318, 2327
BceAI	ACGGC	3 cut(s)	79, 347, 2960
BcgI	CGANNNNNNTGC	2 cut(s)	424, 458
BciT130I	CCWGG	9 cut(s)	1185, 1558, 1662, 2059, 2103, 2263, 2708, 2784, 2859
BclI	TGATCA	2 cut(s)	2866, 2962
BcoDI	GTCTC	7 cut(s)	9, 732, 1046, 1059, 2120, 2214, 2867
BfaI	CTAG	3 cut(s)	159, 2013, 3062
BfmI	CTRYAG	3 cut(s)	907, 1452, 1485
BfoI	RGCGCY	2 cut(s)	779, 3033
BfuAI	ACCTGC	1 cut(s)	1559
BglII	AGATCT	2 cut(s)	976, 2977
BisI	GCNGC	9 cut(s)	94, 163, 236, 356, 381, 719, 1764, 1803, 3046
BlnI	CCTAGG	1 cut(s)	2012
BlpI	GCTNAGC	1 cut(s)	2079
BlsI	GCNGC	9 cut(s)	95, 164, 237, 357, 382, 720, 1765, 1804, 3047
Bme1390I	CCNGG	9 cut(s)	1185, 1558, 1662, 2059, 2103, 2263, 2708, 2784, 2859
Bme18I	GGWCC	5 cut(s)	226, 645, 1250, 1344, 1419
BmeT110I	CYCGRG	1 cut(s)	230
BmgT120I	GGNCC	9 cut(s)	85, 226, 645, 878, 1250, 1344, 1419, 1958, 2336
BmiI	GGNNCC	5 cut(s)	599, 795, 1233, 1345, 1388
BmrFI	CCNGG	9 cut(s)	1185, 1558, 1662, 2059, 2103, 2263, 2708, 2784, 2859
BmrI	ACTGGG	2 cut(s)	1165, 1234
BmsI	GCATC	8 cut(s)	466, 616, 926, 1423, 2065, 2464, 2959, 3111
BmuI	ACTGGG	2 cut(s)	1165, 1234
BpiI	GAAGAC	2 cut(s)	1596, 2024
BplI	GAGNNNNNCTC	2 cut(s)	2914, 2946
BpmI	CTGGAG	2 cut(s)	1566, 1858
Bpu1102I	GCTNAGC	1 cut(s)	2079
Bpu14I	TTCGAA	1 cut(s)	664
BpuEI	CTTGAG	1 cut(s)	1735
BsaAI	YACGTR	2 cut(s)	1808, 1901
BsaBI	GATNNNNATC	1 cut(s)	3097
BsaWI	WCCGGW	2 cut(s)	1818, 1861
Bsc4I	CCNNNNNNNGG	7 cut(s)	193, 543, 790, 1147, 1672, 1955, 2837
Bse118I	RCCGGY	1 cut(s)	110
Bse1I	ACTGG	5 cut(s)	618, 805, 1160, 1229, 1516
Bse21I	CCTNAGG	1 cut(s)	1146
Bse3DI	GCAATG	4 cut(s)	463, 751, 1783, 2607
Bse8I	GATNNNNATC	1 cut(s)	3097
BseAI	TCCGGA	1 cut(s)	1861
BseBI	CCWGG	9 cut(s)	1185, 1558, 1662, 2059, 2103, 2263, 2708, 2784, 2859
BseGI	GGATG	9 cut(s)	631, 1432, 1438, 1549, 1870, 1914, 2335, 2440, 2581
BseJI	GATNNNNATC	1 cut(s)	3097
BseLI	CCNNNNNNNGG	7 cut(s)	193, 543, 790, 1147, 1672, 1955, 2837
BseMI	GCAATG	4 cut(s)	463, 751, 1783, 2607
BseNI	ACTGG	5 cut(s)	618, 805, 1160, 1229, 1516
BseRI	GAGGAG	7 cut(s)	181, 347, 350, 713, 1855, 3008, 3123
BseXI	GCAGC	6 cut(s)	174, 222, 342, 730, 1775, 1789
BsgI	GTGCAG	3 cut(s)	432, 594, 1788
Bsh1236I	CGCG	1 cut(s)	96
BshFI	GGCC	6 cut(s)	86, 879, 1665, 1959, 2337, 2820
BsiHKCI	CYCGRG	1 cut(s)	230
BsiSI	CCGG	3 cut(s)	111, 1819, 1862
BslFI	GGGAC	6 cut(s)	534, 766, 1357, 2119, 2308, 2698
BslI	CCNNNNNNNGG	7 cut(s)	193, 543, 790, 1147, 1672, 1955, 2837
BsmAI	GTCTC	7 cut(s)	9, 732, 1046, 1059, 2120, 2214, 2867
BsmFI	GGGAC	6 cut(s)	534, 766, 1357, 2119, 2308, 2698
BsnI	GGCC	6 cut(s)	86, 879, 1665, 1959, 2337, 2820
BsoBI	CYCGRG	1 cut(s)	230
Bsp119I	TTCGAA	1 cut(s)	664
Bsp13I	TCCGGA	1 cut(s)	1861
Bsp1720I	GCTNAGC	1 cut(s)	2079
Bsp19I	CCATGG	1 cut(s)	1253
BspACI	CCGC	7 cut(s)	91, 94, 241, 314, 381, 1918, 3045
BspANI	GGCC	6 cut(s)	86, 879, 1665, 1959, 2337, 2820
BspEI	TCCGGA	1 cut(s)	1861
BspFNI	CGCG	1 cut(s)	96
BspHI	TCATGA	4 cut(s)	481, 847, 2869, 2965
BspLI	GGNNCC	5 cut(s)	599, 795, 1233, 1345, 1388
BspMI	ACCTGC	1 cut(s)	1559
BspPI	GGATC	8 cut(s)	419, 788, 801, 1113, 1226, 1239, 2742, 2876
BspT104I	TTCGAA	1 cut(s)	664
BsrDI	GCAATG	4 cut(s)	463, 751, 1783, 2607
BsrFI	RCCGGY	1 cut(s)	110
BsrI	ACTGG	5 cut(s)	618, 805, 1160, 1229, 1516
BssAI	RCCGGY	1 cut(s)	110
BssSI	CACGAG	1 cut(s)	798
BssT1I	CCWWGG	5 cut(s)	827, 1253, 1422, 1954, 2012
Bst2BI	CACGAG	1 cut(s)	798
Bst2UI	CCWGG	9 cut(s)	1185, 1558, 1662, 2059, 2103, 2263, 2708, 2784, 2859
Bst4CI	ACNGT	4 cut(s)	2225, 2282, 2650, 2726
Bst6I	CTCTTC	5 cut(s)	510, 1737, 1881, 2672, 2915
BstAPI	GCANNNNNTGC	1 cut(s)	1442
BstBAI	YACGTR	2 cut(s)	1808, 1901
BstBI	TTCGAA	1 cut(s)	664
BstC8I	GCNNGC	6 cut(s)	360, 1375, 2584, 2763, 2773, 2928
BstDSI	CCRYGG	2 cut(s)	1253, 2898
BstEII	GGTNACC	1 cut(s)	502
BstENI	CCTNNNNNAGG	1 cut(s)	1145
BstF5I	GGATG	9 cut(s)	631, 1432, 1438, 1549, 1870, 1914, 2335, 2440, 2581
BstFNI	CGCG	1 cut(s)	96
BstH2I	RGCGCY	2 cut(s)	779, 3033
BstHHI	GCGC	3 cut(s)	98, 778, 3032
BstMAI	GTCTC	7 cut(s)	9, 732, 1046, 1059, 2120, 2214, 2867
BstMWI	GCNNNNNNNGC	6 cut(s)	261, 286, 1374, 1442, 1457, 2699
BstNI	CCWGG	9 cut(s)	1185, 1558, 1662, 2059, 2103, 2263, 2708, 2784, 2859
BstNSI	RCATGY	4 cut(s)	899, 2322, 2930, 2951
BstPI	GGTNACC	1 cut(s)	502
BstSCI	CCNGG	9 cut(s)	1183, 1556, 1660, 2057, 2101, 2261, 2706, 2782, 2857
BstSFI	CTRYAG	3 cut(s)	907, 1452, 1485
BstSNI	TACGTA	1 cut(s)	1901
BstUI	CGCG	1 cut(s)	96
BstV1I	GCAGC	6 cut(s)	174, 222, 342, 730, 1775, 1789
BstV2I	GAAGAC	2 cut(s)	1596, 2024
BstX2I	RGATCY	5 cut(s)	793, 976, 1105, 1231, 2977
BstYI	RGATCY	5 cut(s)	793, 976, 1105, 1231, 2977
Bsu36I	CCTNAGG	1 cut(s)	1146
BsuRI	GGCC	6 cut(s)	86, 879, 1665, 1959, 2337, 2820
BtgI	CCRYGG	2 cut(s)	1253, 2898
BtgZI	GCGATG	2 cut(s)	3063, 3106
BtsCI	GGATG	9 cut(s)	631, 1432, 1438, 1549, 1870, 1914, 2335, 2440, 2581
BtsI	GCAGTG	1 cut(s)	1344
BtsIMutI	CAGTG	5 cut(s)	611, 1344, 1370, 2385, 2526
BveI	ACCTGC	1 cut(s)	1559
Cac8I	GCNNGC	6 cut(s)	360, 1375, 2584, 2763, 2773, 2928
CaiI	CAGNNNCTG	1 cut(s)	355
CciI	TCATGA	4 cut(s)	481, 847, 2869, 2965
CfoI	GCGC	3 cut(s)	98, 778, 3032
Cfr10I	RCCGGY	1 cut(s)	110
Cfr13I	GGNCC	9 cut(s)	85, 226, 645, 878, 1250, 1344, 1419, 1958, 2336
CseI	GACGC	4 cut(s)	361, 2174, 2354, 2588
CsiI	ACCWGGT	1 cut(s)	2782
Csp6I	GTAC	7 cut(s)	303, 1515, 2237, 2243, 2417, 2558, 3036
CspCI	CAANNNNNGTGG	2 cut(s)	520, 555
CviQI	GTAC	7 cut(s)	303, 1515, 2237, 2243, 2417, 2558, 3036
Eam1104I	CTCTTC	5 cut(s)	510, 1737, 1881, 2672, 2915
EarI	CTCTTC	5 cut(s)	510, 1737, 1881, 2672, 2915
EciI	GGCGGA	2 cut(s)	80, 303
Eco105I	TACGTA	1 cut(s)	1901
Eco130I	CCWWGG	5 cut(s)	827, 1253, 1422, 1954, 2012
Eco47I	GGWCC	5 cut(s)	226, 645, 1250, 1344, 1419
Eco47III	AGCGCT	1 cut(s)	3031
Eco57I	CTGAAG	4 cut(s)	311, 734, 1758, 2501
Eco72I	CACGTG	1 cut(s)	1808
Eco81I	CCTNAGG	1 cut(s)	1146
Eco88I	CYCGRG	1 cut(s)	230
Eco91I	GGTNACC	1 cut(s)	502
EcoNI	CCTNNNNNAGG	1 cut(s)	1145
EcoO109I	RGGNCCY	3 cut(s)	85, 226, 645
EcoO65I	GGTNACC	1 cut(s)	502
EcoRI	GAATTC	1 cut(s)	2911
EcoRII	CCWGG	9 cut(s)	1183, 1556, 1660, 2057, 2101, 2261, 2706, 2782, 2857
EcoT14I	CCWWGG	5 cut(s)	827, 1253, 1422, 1954, 2012
ErhI	CCWWGG	5 cut(s)	827, 1253, 1422, 1954, 2012
FaqI	GGGAC	6 cut(s)	534, 766, 1357, 2119, 2308, 2698
FbaI	TGATCA	2 cut(s)	2866, 2962
FblI	GTMKAC	2 cut(s)	183, 2788
Fnu4HI	GCNGC	9 cut(s)	94, 163, 236, 356, 381, 719, 1764, 1803, 3046
FokI	GGATG	9 cut(s)	638, 1439, 1445, 1536, 1877, 1901, 2342, 2447, 2588
Fsp4HI	GCNGC	9 cut(s)	94, 163, 236, 356, 381, 719, 1764, 1803, 3046
FspBI	CTAG	3 cut(s)	159, 2013, 3062
GlaI	GCGC	3 cut(s)	97, 777, 3031
GluI	GCNGC	9 cut(s)	94, 163, 236, 356, 381, 719, 1764, 1803, 3046
GsuI	CTGGAG	2 cut(s)	1566, 1858
HaeII	RGCGCY	2 cut(s)	779, 3033
HaeIII	GGCC	6 cut(s)	86, 879, 1665, 1959, 2337, 2820
HapII	CCGG	3 cut(s)	111, 1819, 1862
HgaI	GACGC	4 cut(s)	361, 2174, 2354, 2588
HhaI	GCGC	3 cut(s)	98, 778, 3032
Hin6I	GCGC	3 cut(s)	96, 776, 3030
HinP1I	GCGC	3 cut(s)	96, 776, 3030
HincII	GTYRAC	3 cut(s)	1153, 1699, 2789
HindII	GTYRAC	3 cut(s)	1153, 1699, 2789
HindIII	AAGCTT	4 cut(s)	1458, 2684, 2742, 2813
HpaI	GTTAAC	1 cut(s)	1153
HpaII	CCGG	3 cut(s)	111, 1819, 1862
HphI	GGTGA	6 cut(s)	100, 124, 496, 1478, 1739, 1808
Hpy166II	GTNNAC	6 cut(s)	184, 1153, 1419, 1578, 1699, 2789
Hpy8I	GTNNAC	6 cut(s)	184, 1153, 1419, 1578, 1699, 2789
HpyCH4III	ACNGT	4 cut(s)	2225, 2282, 2650, 2726
HpyCH4IV	ACGT	5 cut(s)	180, 373, 1807, 1900, 1945
HpyF10VI	GCNNNNNNNGC	6 cut(s)	261, 286, 1374, 1442, 1457, 2699
HpySE526I	ACGT	5 cut(s)	180, 373, 1807, 1900, 1945
HspAI	GCGC	3 cut(s)	96, 776, 3030
Kpn2I	TCCGGA	1 cut(s)	1861
Ksp22I	TGATCA	2 cut(s)	2866, 2962
KspAI	GTTAAC	1 cut(s)	1153
LmnI	GCTCC	3 cut(s)	243, 1159, 1386
Lsp1109I	GCAGC	6 cut(s)	174, 222, 342, 730, 1775, 1789
LweI	GCATC	8 cut(s)	466, 616, 926, 1423, 2065, 2464, 2959, 3111
MabI	ACCWGGT	1 cut(s)	2782
MaeI	CTAG	3 cut(s)	159, 2013, 3062
MaeII	ACGT	5 cut(s)	180, 373, 1807, 1900, 1945
MaeIII	GTNAC	5 cut(s)	502, 1984, 2366, 2507, 2567
MfeI	CAATTG	1 cut(s)	1767
MflI	RGATCY	5 cut(s)	793, 976, 1105, 1231, 2977
MlyI	GAGTC	6 cut(s)	203, 1061, 1348, 1573, 1742, 2720
MmeI	TCCRAC	4 cut(s)	231, 351, 1577, 1616
MroI	TCCGGA	1 cut(s)	1861
MroXI	GAANNNNTTC	2 cut(s)	374, 489
MslI	CAYNNNNRTG	3 cut(s)	1363, 2459, 2600
MspA1I	CMGCKG	1 cut(s)	3045
MspI	CCGG	3 cut(s)	111, 1819, 1862
MspR9I	CCNGG	9 cut(s)	1185, 1558, 1662, 2059, 2103, 2263, 2708, 2784, 2859
MunI	CAATTG	1 cut(s)	1767
MvaI	CCWGG	9 cut(s)	1185, 1558, 1662, 2059, 2103, 2263, 2708, 2784, 2859
MvnI	CGCG	1 cut(s)	96
MwoI	GCNNNNNNNGC	6 cut(s)	261, 286, 1374, 1442, 1457, 2699
NcoI	CCATGG	1 cut(s)	1253
NlaIV	GGNNCC	5 cut(s)	599, 795, 1233, 1345, 1388
NmeAIII	GCCGAG	2 cut(s)	40, 173
NmuCI	GTSAC	3 cut(s)	502, 2366, 2507
NspI	RCATGY	4 cut(s)	899, 2322, 2930, 2951
NspV	TTCGAA	1 cut(s)	664
PaeI	GCATGC	1 cut(s)	2930
PaeR7I	CTCGAG	1 cut(s)	230
PagI	TCATGA	4 cut(s)	481, 847, 2869, 2965
PciI	ACATGT	2 cut(s)	895, 2318
PcsI	WCGNNNNNNNCGW	7 cut(s)	18, 45, 48, 57, 78, 228, 384
PdmI	GAANNNNTTC	2 cut(s)	374, 489
PfeI	GAWTC	6 cut(s)	557, 836, 1037, 1279, 1625, 3098
PflMI	CCANNNNNTGG	2 cut(s)	543, 1955
PfoI	TCCNGGA	2 cut(s)	2706, 2857
PkrI	GCNGC	9 cut(s)	95, 164, 237, 357, 382, 720, 1765, 1804, 3047
PleI	GAGTC	6 cut(s)	203, 1061, 1348, 1573, 1742, 2719
PmaCI	CACGTG	1 cut(s)	1808
PmlI	CACGTG	1 cut(s)	1808
PpsI	GAGTC	6 cut(s)	203, 1061, 1348, 1573, 1742, 2719
Ppu21I	YACGTR	2 cut(s)	1808, 1901
PpuMI	RGGWCCY	2 cut(s)	226, 645
PscI	ACATGT	2 cut(s)	895, 2318
PshBI	ATTAAT	1 cut(s)	633
Psp1406I	AACGTT	1 cut(s)	373
Psp5II	RGGWCCY	2 cut(s)	226, 645
Psp6I	CCWGG	9 cut(s)	1183, 1556, 1660, 2057, 2101, 2261, 2706, 2782, 2857
PspCI	CACGTG	1 cut(s)	1808
PspEI	GGTNACC	1 cut(s)	502
PspGI	CCWGG	9 cut(s)	1183, 1556, 1660, 2057, 2101, 2261, 2706, 2782, 2857
PspN4I	GGNNCC	5 cut(s)	599, 795, 1233, 1345, 1388
PspPI	GGNCC	9 cut(s)	85, 226, 645, 878, 1250, 1344, 1419, 1958, 2336
PspPPI	RGGWCCY	2 cut(s)	226, 645
PspXI	VCTCGAGB	1 cut(s)	230
PstNI	CAGNNNCTG	1 cut(s)	355
PsuI	RGATCY	5 cut(s)	793, 976, 1105, 1231, 2977
RsaI	GTAC	7 cut(s)	304, 1516, 2238, 2244, 2418, 2559, 3037
RsaNI	GTAC	7 cut(s)	303, 1515, 2237, 2243, 2417, 2558, 3036
RseI	CAYNNNNRTG	3 cut(s)	1363, 2459, 2600
SalI	GTCGAC	1 cut(s)	2787
SatI	GCNGC	9 cut(s)	94, 163, 236, 356, 381, 719, 1764, 1803, 3046
Sau96I	GGNCC	9 cut(s)	85, 226, 645, 878, 1250, 1344, 1419, 1958, 2336
SchI	GAGTC	6 cut(s)	203, 1061, 1348, 1573, 1742, 2720
ScrFI	CCNGG	9 cut(s)	1185, 1558, 1662, 2059, 2103, 2263, 2708, 2784, 2859
SexAI	ACCWGGT	1 cut(s)	2782
SfaNI	GCATC	8 cut(s)	466, 616, 926, 1423, 2065, 2464, 2959, 3111
SfcI	CTRYAG	3 cut(s)	907, 1452, 1485
Sfr274I	CTCGAG	1 cut(s)	230
SfuI	TTCGAA	1 cut(s)	664
SinI	GGWCC	5 cut(s)	226, 645, 1250, 1344, 1419
SlaI	CTCGAG	1 cut(s)	230
SmiMI	CAYNNNNRTG	3 cut(s)	1363, 2459, 2600
SmlI	CTYRAG	2 cut(s)	230, 1750
SmoI	CTYRAG	2 cut(s)	230, 1750
SnaBI	TACGTA	1 cut(s)	1901
SphI	GCATGC	1 cut(s)	2930
SsiI	CCGC	7 cut(s)	91, 94, 241, 314, 381, 1918, 3045
SspI	AATATT	1 cut(s)	1327
SspMI	CTAG	3 cut(s)	159, 2013, 3062
StyD4I	CCNGG	9 cut(s)	1183, 1556, 1660, 2057, 2101, 2261, 2706, 2782, 2857
StyI	CCWWGG	5 cut(s)	827, 1253, 1422, 1954, 2012
TaaI	ACNGT	4 cut(s)	2225, 2282, 2650, 2726
TaiI	ACGT	5 cut(s)	183, 376, 1810, 1903, 1948
TatI	WGTACW	1 cut(s)	302
TauI	GCSGC	3 cut(s)	96, 383, 3048
TfiI	GAWTC	6 cut(s)	557, 836, 1037, 1279, 1625, 3098
TscAI	CASTG	5 cut(s)	618, 1344, 1370, 2392, 2533
TseFI	GTSAC	3 cut(s)	502, 2366, 2507
TseI	GCWGC	6 cut(s)	162, 235, 355, 718, 1763, 1802
Tsp45I	GTSAC	3 cut(s)	502, 2366, 2507
TspDTI	ATGAA	9 cut(s)	474, 498, 528, 927, 1538, 1881, 2009, 2223, 2652
TspGWI	ACGGA	2 cut(s)	1018, 1518
TspRI	CASTG	5 cut(s)	618, 1344, 1370, 2392, 2533
Van91I	CCANNNNNTGG	2 cut(s)	543, 1955
VpaK11BI	GGWCC	5 cut(s)	226, 645, 1250, 1344, 1419
VspI	ATTAAT	1 cut(s)	633
XagI	CCTNNNNNAGG	1 cut(s)	1145
XapI	RAATTY	6 cut(s)	889, 926, 1086, 2286, 2850, 2911
XceI	RCATGY	4 cut(s)	899, 2322, 2930, 2951
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1301
XhoI	CTCGAG	1 cut(s)	230
XmaJI	CCTAGG	1 cut(s)	2012
XmiI	GTMKAC	2 cut(s)	183, 2788
XmnI	GAANNNNTTC	2 cut(s)	374, 489
XspI	CTAG	3 cut(s)	159, 2013, 3062

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.