Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0000712

Physical Location & Seq

Genomic Coordinates Reverse (-)

18167865 .. 18171992

Gene Structure 4128 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00178510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1350 bp

ATGGAAAGCCAGCAACAGAGAATAGGTGTTGGTGAGCGTGGAAGCAACCGCATCTATTCAGCAGAGGTCGAGACACCACCACCCTCCGATATCAAACAATTTGATGACCATTATGATGATCCTGAAAATCAGAAGCCTGGATGGAGGAAGTTTCTGGCACATGTAGGACCTGGGTTTCTCGTCTCCTTGGCTTACCTTGACCCCGGCAACTGCAAACATTTGTCGGAATTATGTAAGGCGGAGTACCCACCATTTGTAAAGTACTGTCTCTGGTTGCTTGCAGAGGTTGCTGTCATAGCTGCTGATATACCCGAAGTGATTGGAACAGCGTTTGCTCTAAATATACTGTTCAACATTCCAGTTTGGACTGGAGTACTATTGACTGGTTTCAGTACTCTCCTTCTTCTTGGCCTGCAGAGATATGGGGTTAGGAAGCTGGAAATGCTGATAGCAGTACTAGTGTTTGTAATGGCAGCCTGTTTCTTTGGTGAAATGGGTTATGTGAAGCCTCCTGCATCCGGTGTGATTGAAGGAATGTTTGTCCCCAAGCTCAGCGGCCAAGGAGCCACCGGTGACGCCATCGCCCTCTTGGGTGCCCTTGTCATGCCCCACAATCTTTTTCTCCACTCTGCTCTTGTGCTTTCAAGGAAAGTCCCGAACTCTGTCCGTGGCATCAATGATGCATGTCGATATTTCTTGATAGAAAGTGGATTTGCATTATTCATAGCATTTCTAATCAATGTTGCCATTGTCTCTGTGTCGGGAACCGTTTGCCACGGAGATAACCTCTCTGACAAGGATGCTAAAACATGTAGCGATCTGACACTCAATTCTGCTTCTTTTCTTCTCCAGAATGTGTTGGGAAAGTCAAGCAAAGTCATCTATGCTATTGCACTATTAGCCTCAGGACAAAGCTCAACCATTACAGGCACTTACGCAGGACAATTTGTCATGCAGGGTTTCTTGGACATTAAGATGAAAAAATGGCTTAGAAACCTGATGACTAGGTGTATTGCCATTACACCTAGTCTTGTTGTTTCCATCATCGGGATGGGAATCATTGGAATCAACATCTATTACCTCAGCACAGGCTTTGTGGGCTGGATAATCCATAGCAGTCTCCCCAAAGTTGCAACTGTGTTTATTGGGATCTTGGTGTTCCCCTTAATGGCAATTTACATCCTTGCAGTCCTTTACTTAACTGTCAGAAAAGACACTGTTATAACATTCATTGAGCCAACAAAGAACGACCCAGCGGCCCAGAACCAAATGGAAAGTGGGATCACCAACACGCATGGACCTCCTGTACCTTTCAGGGAGGATTTGGCTGACGTTCCTCTACCAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0010035 GO:0010038 GO:0010044 GO:0015075 GO:0015083 GO:0015318 GO:0015690 GO:0016020 GO:0022857 GO:0030001 GO:0034220 GO:0042221 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0071944 GO:0072512 GO:0098660 GO:1902602

KEGG Pathways

Metabolic & Signaling

ko04142 ko04216 ko04978 map04142 map04216 map04978

Pfam Domains

Protein Families

Nramp

Protein Analysis

449

Amino Acids

48.81

Weight (kDa)

6.46

Isoelectric Point (pI)

36.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nramp	PF01566	72 - 363	2.2e-83	Natural resistance-associated macrophage protein-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000712)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15960 AT1G80830 AT1G80830
fragaria_vesca	FvH4_2g27530 FvH4_6g28670 FvH4_6g28670 FvH4_6g28670 FvH4_6g28670 FvH4_6g28670 FvH4_6g28670 FvH4_6g28670 FvH4_6g28670 FvH4_6g28670 FvH4_6g28670
malus_domestica	MD00G1030300.v1.1 MD00G1042000.v1.1 MD14G1158400.v1.1 MD17G1222500.v1.1
prunus_persica	Prupe.1G492500_v2.0.a1 Prupe.3G097800_v2.0.a1 Prupe.3G097800_v2.0.a1 Prupe.3G097800_v2.0.a1 Prupe.3G097800_v2.0.a1 Prupe.3G097800_v2.0.a1 Prupe.3G097800_v2.0.a1 Prupe.3G097800_v2.0.a1 Prupe.3G097800_v2.0.a1 Prupe.3G097800_v2.0.a1
pyrus_communis	pycom116g00050 pycom14g13220 pycom17g22680
rosa_chinensis	RchiOBHm_Chr2g0133071 RchiOBHm_Chr6g0271151 RchiOBHm_Chr6g0296361 RchiOBHm_Chr6g0310261
rosa_laevigata	RLG00000011698 RLG00000011701 RLG00000019306
rosa_multiflora	Rmu_sc0000127.1_g000013 Rmu_sc0000279.1_g000004 Rmu_sc0008096.1_g000001 Rmu_sc0037233.1_g000001
rosa_roxburghii	Rroxscaffold_2G00111550 Rroxscaffold_2G00136120 Rroxscaffold_7G00176290 Rroxscaffold_7G00178510
rosa_rugosa	Rorug01G0010700 Rorug01G0010800 Rorug01G0062600 Rorug01G0087600 Rorug02G0179300 Rorug02G0309300 Rorug02G0309400 Rorug02G0309500 Rorug02G0309600 Rorug03G0345700 Rorug05G0313000 Rorug06G0261600.1
rosa_samantha	Rh2AG361900 Rh2BG367600 Rh2CG345100 Rh2DG384500 Rh3AG218600 Rh4AG271200 Rh5AG321100 Rh6AG181200 Rh6AG373200 Rh6AG492800 Rh6CG386600 Rh7DG314800
rosa_wichuraiana	Rw2G029420 Rw6G032540 Rw6G045020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1223
AccB1I	GGYRCC	1 cut(s)	593
AciI	CCGC	4 cut(s)	49, 239, 555, 1256
AclWI	GGATC	3 cut(s)	113, 1157, 1289
AcoI	YGGCCR	1 cut(s)	556
AcyI	GRCGYC	1 cut(s)	576
AfaI	GTAC	6 cut(s)	245, 263, 375, 394, 456, 1308
AfiI	CCNNNNNNNGG	3 cut(s)	518, 1047, 1168
AflIII	ACRYGT	2 cut(s)	160, 809
AgeI	ACCGGT	1 cut(s)	569
AgsI	TTSAA	3 cut(s)	352, 530, 645
AhdI	GACNNNNNGTC	1 cut(s)	947
AhlI	ACTAGT	1 cut(s)	457
AjnI	CCWGG	2 cut(s)	136, 169
AluBI	AGCT	4 cut(s)	299, 436, 550, 915
AluI	AGCT	4 cut(s)	299, 436, 550, 915
Alw26I	GTCTC	5 cut(s)	65, 187, 272, 757, 1124
AlwI	GGATC	3 cut(s)	113, 1157, 1289
AoxI	GGCC	3 cut(s)	409, 556, 1257
ApeKI	GCWGC	2 cut(s)	299, 473
AsiGI	ACCGGT	1 cut(s)	569
AspS9I	GGNCC	3 cut(s)	167, 1258, 1298
AsuC2I	CCSGG	1 cut(s)	204
AsuHPI	GGTGA	4 cut(s)	44, 500, 584, 1276
AvaII	GGWCC	2 cut(s)	167, 1298
AxyI	CCTNAGG	1 cut(s)	904
BaeGI	GKGCMC	1 cut(s)	598
BanI	GGYRCC	1 cut(s)	593
BbvCI	CCTCAGC	1 cut(s)	1082
BbvI	GCAGC	2 cut(s)	286, 485
BccI	CCATC	4 cut(s)	135, 587, 1045, 1049
BciT130I	CCWGG	2 cut(s)	138, 171
BcnI	CCSGG	1 cut(s)	204
BcoDI	GTCTC	5 cut(s)	65, 187, 272, 757, 1124
BcuI	ACTAGT	1 cut(s)	457
BfaI	CTAG	3 cut(s)	458, 1005, 1026
BfmI	CTRYAG	1 cut(s)	413
BisI	GCNGC	4 cut(s)	300, 474, 556, 1257
BlpI	GCTNAGC	1 cut(s)	551
BlsI	GCNGC	4 cut(s)	301, 475, 557, 1258
BmcAI	AGTACT	4 cut(s)	263, 375, 394, 456
Bme1390I	CCNGG	3 cut(s)	138, 171, 204
Bme18I	GGWCC	2 cut(s)	167, 1298
BmeRI	GACNNNNNGTC	1 cut(s)	947
BmgT120I	GGNCC	3 cut(s)	167, 1258, 1298
BmiI	GGNNCC	3 cut(s)	565, 595, 766
BmrFI	CCNGG	3 cut(s)	138, 171, 204
BmsI	GCATC	5 cut(s)	60, 524, 670, 681, 790
BplI	GAGNNNNNCTC	2 cut(s)	771, 803
BpmI	CTGGAG	2 cut(s)	390, 833
Bpu10I	CCTNAGC	1 cut(s)	1082
Bpu1102I	GCTNAGC	1 cut(s)	551
BpuMI	CCSGG	1 cut(s)	204
BsaBI	GATNNNNATC	1 cut(s)	1055
BsaHI	GRCGYC	1 cut(s)	576
BsaJI	CCNNGG	6 cut(s)	170, 186, 202, 559, 667, 775
BsaWI	WCCGGW	2 cut(s)	518, 569
Bsc4I	CCNNNNNNNGG	3 cut(s)	518, 1047, 1168
Bse118I	RCCGGY	1 cut(s)	569
Bse1I	ACTGG	3 cut(s)	359, 373, 388
Bse21I	CCTNAGG	1 cut(s)	904
Bse8I	GATNNNNATC	1 cut(s)	1055
BseBI	CCWGG	2 cut(s)	138, 171
BseDI	CCNNGG	6 cut(s)	170, 186, 202, 559, 667, 775
BseGI	GGATG	5 cut(s)	146, 515, 805, 1056, 1179
BseJI	GATNNNNATC	1 cut(s)	1055
BseLI	CCNNNNNNNGG	3 cut(s)	518, 1047, 1168
BseMII	CTCAG	3 cut(s)	565, 918, 1096
BseNI	ACTGG	3 cut(s)	359, 373, 388
BseSI	GKGCMC	1 cut(s)	598
BseXI	GCAGC	2 cut(s)	286, 485
BseYI	CCCAGC	1 cut(s)	1252
BshFI	GGCC	3 cut(s)	411, 558, 1259
BshNI	GGYRCC	1 cut(s)	593
BshTI	ACCGGT	1 cut(s)	569
BsiSI	CCGG	3 cut(s)	204, 519, 570
BslFI	GGGAC	2 cut(s)	527, 638
BslI	CCNNNNNNNGG	3 cut(s)	518, 1047, 1168
BsmAI	GTCTC	5 cut(s)	65, 187, 272, 757, 1124
BsmBI	CGTCTC	1 cut(s)	187
BsmFI	GGGAC	2 cut(s)	527, 638
BsnI	GGCC	3 cut(s)	411, 558, 1259
Bsp1286I	GDGCHC	1 cut(s)	598
Bsp143I	GATC	4 cut(s)	118, 817, 1149, 1281
Bsp1720I	GCTNAGC	1 cut(s)	551
BspACI	CCGC	4 cut(s)	49, 239, 555, 1256
BspANI	GGCC	3 cut(s)	411, 558, 1259
BspCNI	CTCAG	3 cut(s)	564, 917, 1095
BspLI	GGNNCC	3 cut(s)	565, 595, 766
BspMAI	CTGCAG	1 cut(s)	417
BspPI	GGATC	3 cut(s)	113, 1157, 1289
BspT107I	GGYRCC	1 cut(s)	593
BsrFI	RCCGGY	1 cut(s)	569
BsrI	ACTGG	3 cut(s)	359, 373, 388
BssAI	RCCGGY	1 cut(s)	569
BssECI	CCNNGG	6 cut(s)	170, 186, 202, 559, 667, 775
BssMI	GATC	4 cut(s)	118, 817, 1149, 1281
BssNI	GRCGYC	1 cut(s)	576
BssT1I	CCWWGG	2 cut(s)	186, 559
Bst2UI	CCWGG	2 cut(s)	138, 171
Bst4CI	ACNGT	6 cut(s)	266, 348, 769, 1138, 1204, 1219
BstACI	GRCGYC	1 cut(s)	576
BstAPI	GCANNNNNTGC	1 cut(s)	287
BstC8I	GCNNGC	3 cut(s)	11, 279, 413
BstDEI	CTNAG	4 cut(s)	551, 904, 989, 1082
BstDSI	CCRYGG	2 cut(s)	667, 775
BstF5I	GGATG	5 cut(s)	146, 515, 805, 1056, 1179
BstKTI	GATC	4 cut(s)	121, 820, 1152, 1284
BstMAI	GTCTC	5 cut(s)	65, 187, 272, 757, 1124
BstMBI	GATC	4 cut(s)	118, 817, 1149, 1281
BstMWI	GCNNNNNNNGC	5 cut(s)	287, 296, 442, 899, 1098
BstNI	CCWGG	2 cut(s)	138, 171
BstNSI	RCATGY	3 cut(s)	164, 687, 813
BstSCI	CCNGG	3 cut(s)	136, 169, 202
BstSFI	CTRYAG	1 cut(s)	413
BstSLI	GKGCMC	1 cut(s)	598
BstV1I	GCAGC	2 cut(s)	286, 485
BstX2I	RGATCY	1 cut(s)	1149
BstYI	RGATCY	1 cut(s)	1149
Bsu36I	CCTNAGG	1 cut(s)	904
BsuRI	GGCC	3 cut(s)	411, 558, 1259
BtgI	CCRYGG	2 cut(s)	667, 775
BtgZI	GCGATG	1 cut(s)	565
BtsCI	GGATG	5 cut(s)	146, 515, 805, 1056, 1179
BtsIMutI	CAGTG	1 cut(s)	1215
Cac8I	GCNNGC	3 cut(s)	11, 279, 413
Cfr10I	RCCGGY	1 cut(s)	569
Cfr13I	GGNCC	3 cut(s)	167, 1258, 1298
CseI	GACGC	1 cut(s)	584
Csp6I	GTAC	6 cut(s)	244, 262, 374, 393, 455, 1307
CspAI	ACCGGT	1 cut(s)	569
CviAII	CATG	6 cut(s)	161, 604, 684, 810, 952, 1295
CviQI	GTAC	6 cut(s)	244, 262, 374, 393, 455, 1307
DdeI	CTNAG	4 cut(s)	551, 904, 989, 1082
DpnI	GATC	4 cut(s)	120, 819, 1151, 1283
DpnII	GATC	4 cut(s)	118, 817, 1149, 1281
DriI	GACNNNNNGTC	1 cut(s)	947
EaeI	YGGCCR	1 cut(s)	556
Eam1105I	GACNNNNNGTC	1 cut(s)	947
EciI	GGCGGA	1 cut(s)	254
Eco130I	CCWWGG	2 cut(s)	186, 559
Eco32I	GATATC	1 cut(s)	91
Eco47I	GGWCC	2 cut(s)	167, 1298
Eco81I	CCTNAGG	1 cut(s)	904
EcoO109I	RGGNCCY	1 cut(s)	167
EcoRII	CCWGG	2 cut(s)	136, 169
EcoRV	GATATC	1 cut(s)	91
EcoT14I	CCWWGG	2 cut(s)	186, 559
EcoT22I	ATGCAT	1 cut(s)	685
ErhI	CCWWGG	2 cut(s)	186, 559
Esp3I	CGTCTC	1 cut(s)	187
FaeI	CATG	6 cut(s)	164, 607, 687, 813, 955, 1298
FaqI	GGGAC	2 cut(s)	527, 638
FatI	CATG	6 cut(s)	160, 603, 683, 809, 951, 1294
Fnu4HI	GCNGC	4 cut(s)	300, 474, 556, 1257
FokI	GGATG	5 cut(s)	153, 502, 812, 1063, 1166
Fsp4HI	GCNGC	4 cut(s)	300, 474, 556, 1257
FspBI	CTAG	3 cut(s)	458, 1005, 1026
GluI	GCNGC	4 cut(s)	300, 474, 556, 1257
GsaI	CCCAGC	1 cut(s)	1256
GsuI	CTGGAG	2 cut(s)	390, 833
HaeIII	GGCC	3 cut(s)	411, 558, 1259
HapII	CCGG	3 cut(s)	204, 519, 570
HgaI	GACGC	1 cut(s)	584
Hin1I	GRCGYC	1 cut(s)	576
Hin1II	CATG	6 cut(s)	164, 607, 687, 813, 955, 1298
HinfI	GANTC	2 cut(s)	1056, 1065
HpaII	CCGG	3 cut(s)	204, 519, 570
HphI	GGTGA	4 cut(s)	44, 500, 584, 1276
Hpy188I	TCNGA	6 cut(s)	88, 132, 226, 793, 822, 1208
Hpy188III	TCNNGA	8 cut(s)	70, 122, 655, 697, 762, 850, 906, 1048
HpyAV	CCTTC	2 cut(s)	410, 524
HpyCH4III	ACNGT	6 cut(s)	266, 348, 769, 1138, 1204, 1219
HpyCH4IV	ACGT	1 cut(s)	1332
HpyF10VI	GCNNNNNNNGC	5 cut(s)	287, 296, 442, 899, 1098
HpyF3I	CTNAG	4 cut(s)	551, 904, 989, 1082
HpySE526I	ACGT	1 cut(s)	1332
Hsp92I	GRCGYC	1 cut(s)	576
Hsp92II	CATG	6 cut(s)	164, 607, 687, 813, 955, 1298
Kzo9I	GATC	4 cut(s)	118, 817, 1149, 1281
LmnI	GCTCC	1 cut(s)	563
Lsp1109I	GCAGC	2 cut(s)	286, 485
LweI	GCATC	5 cut(s)	60, 524, 670, 681, 790
MaeI	CTAG	3 cut(s)	458, 1005, 1026
MaeII	ACGT	1 cut(s)	1332
MaeIII	GTNAC	1 cut(s)	572
MalI	GATC	4 cut(s)	120, 819, 1151, 1283
MboI	GATC	4 cut(s)	118, 817, 1149, 1281
MboII	GAAGA	2 cut(s)	395, 836
MflI	RGATCY	1 cut(s)	1149
MhlI	GDGCHC	1 cut(s)	598
MluCI	AATT	5 cut(s)	98, 227, 829, 944, 1173
MmeI	TCCRAC	1 cut(s)	204
Mph1103I	ATGCAT	1 cut(s)	685
MseI	TTAA	3 cut(s)	972, 1166, 1199
MslI	CAYNNNNRTG	3 cut(s)	114, 974, 1049
MspA1I	CMGCKG	2 cut(s)	555, 1256
MspI	CCGG	3 cut(s)	204, 519, 570
MspR9I	CCNGG	3 cut(s)	138, 171, 204
MvaI	CCWGG	2 cut(s)	138, 171
MwoI	GCNNNNNNNGC	5 cut(s)	287, 296, 442, 899, 1098
NciI	CCSGG	1 cut(s)	204
NdeII	GATC	4 cut(s)	118, 817, 1149, 1281
NlaIII	CATG	6 cut(s)	164, 607, 687, 813, 955, 1298
NlaIV	GGNNCC	3 cut(s)	565, 595, 766
NmuCI	GTSAC	1 cut(s)	572
NsiI	ATGCAT	1 cut(s)	685
NspI	RCATGY	3 cut(s)	164, 687, 813
PciI	ACATGT	2 cut(s)	160, 809
PfeI	GAWTC	2 cut(s)	1056, 1065
PinAI	ACCGGT	1 cut(s)	569
PkrI	GCNGC	4 cut(s)	301, 475, 557, 1258
PpuMI	RGGWCCY	1 cut(s)	167
PscI	ACATGT	2 cut(s)	160, 809
PsiI	TTATAA	1 cut(s)	1223
Psp5II	RGGWCCY	1 cut(s)	167
Psp6I	CCWGG	2 cut(s)	136, 169
PspFI	CCCAGC	1 cut(s)	1252
PspGI	CCWGG	2 cut(s)	136, 169
PspN4I	GGNNCC	3 cut(s)	565, 595, 766
PspPI	GGNCC	3 cut(s)	167, 1258, 1298
PspPPI	RGGWCCY	1 cut(s)	167
PstI	CTGCAG	1 cut(s)	417
PsuI	RGATCY	1 cut(s)	1149
RsaI	GTAC	6 cut(s)	245, 263, 375, 394, 456, 1308
RsaNI	GTAC	6 cut(s)	244, 262, 374, 393, 455, 1307
RseI	CAYNNNNRTG	3 cut(s)	114, 974, 1049
SaqAI	TTAA	3 cut(s)	972, 1166, 1199
SatI	GCNGC	4 cut(s)	300, 474, 556, 1257
Sau3AI	GATC	4 cut(s)	118, 817, 1149, 1281
Sau96I	GGNCC	3 cut(s)	167, 1258, 1298
ScaI	AGTACT	4 cut(s)	263, 375, 394, 456
ScrFI	CCNGG	3 cut(s)	138, 171, 204
SduI	GDGCHC	1 cut(s)	598
SfaNI	GCATC	5 cut(s)	60, 524, 670, 681, 790
SfcI	CTRYAG	1 cut(s)	413
SgrAI	CRCCGGYG	1 cut(s)	569
SinI	GGWCC	2 cut(s)	167, 1298
SmiMI	CAYNNNNRTG	3 cut(s)	114, 974, 1049
SpeI	ACTAGT	1 cut(s)	457
Sse9I	AATT	5 cut(s)	98, 227, 829, 944, 1173
SsiI	CCGC	4 cut(s)	49, 239, 555, 1256
SspMI	CTAG	3 cut(s)	458, 1005, 1026
StyD4I	CCNGG	3 cut(s)	136, 169, 202
StyI	CCWWGG	2 cut(s)	186, 559
TaaI	ACNGT	6 cut(s)	266, 348, 769, 1138, 1204, 1219
TaiI	ACGT	1 cut(s)	1335
TaqI	TCGA	2 cut(s)	69, 688
TasI	AATT	5 cut(s)	98, 227, 829, 944, 1173
TatI	WGTACW	4 cut(s)	261, 373, 392, 454
TauI	GCSGC	2 cut(s)	558, 1259
TfiI	GAWTC	2 cut(s)	1056, 1065
Tru1I	TTAA	3 cut(s)	972, 1166, 1199
Tru9I	TTAA	3 cut(s)	972, 1166, 1199
TscAI	CASTG	1 cut(s)	1222
TseFI	GTSAC	1 cut(s)	572
TseI	GCWGC	2 cut(s)	299, 473
Tsp45I	GTSAC	1 cut(s)	572
TspDTI	ATGAA	3 cut(s)	712, 992, 1219
TspGWI	ACGGA	2 cut(s)	656, 792
TspRI	CASTG	1 cut(s)	1222
VpaK11BI	GGWCC	2 cut(s)	167, 1298
XceI	RCATGY	3 cut(s)	164, 687, 813
XcmI	CCANNNNNNNNNTGG	3 cut(s)	586, 1048, 1274
XspI	CTAG	3 cut(s)	458, 1005, 1026
ZrmI	AGTACT	4 cut(s)	263, 375, 394, 456
Zsp2I	ATGCAT	1 cut(s)	685

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.