Rorug01G0004700

Alcohol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001983
Physical Location & Seq
Reverse (-)
994227 .. 999501
5275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0004700.1

Sequence Viewer

Length: 771 bp
ATGGCTATAGATCCCAACCACGCCACCGCCACCGCCACCGCCACCGGCGCCGCCGCCACACGGCCGCTGAGACCCATAGCTCCCGCACCTCCTCCTGCTCATATGTACACGGTCCCTATTCGGGCCCAATCTTCACCTGGTGCACTTGTCTACCCATCTGCCCGCGCTCCCTACCCATCTCCCCTCAATTTTCATTCCCATCCTTATTCTCCTCATCTTCATGCTCCTCCTCCTCCTCCTCTTCCCTCGTACCAATCTCCTCTTCCGCCTCCAATTAAGCCCGACCTTCGCTTTCCTGGCCTCGTTGCTCCTCCGTCTTCAGTTCCCGACTCCAATGGCATCAGGGATAAAAGCAGAGATGACACTCTTTCTGTCATTCAAGATCGGAAAGTCAGGATAACAGATGGAGCTTCTCTATATGCGCTTTGTCGGTCATGGCTGAGGAACGGTTCTCCCGAAGAAAGTCAGACACGATATGGTGATGCTACAAGGTCTCTTCCAAAGCCTTCACCCATACCTATGGCAAGTGTGACTCCGCCAAAGAAGGAAGAAGGCGATAAAAAAGAAGACATTGAAGACAAGGATGAGGAATCCCTTGAACATGTATCACCAGAAGATCTGTTAAAAAGACATGTCAAACGTGCTAGAAAGGTTCGAGCACGATTAAGAGAGGAACGTTTGCGGCGCATTGCAAGGTACAAGTCTAGGCTTGCTCTTCTCCTTCCTCCACTGGTAGAACAGTTCCGAAATGATCTAGCTGCTGGAAATTAG

Protein Analysis

256

Amino Acids

28.22

Weight (kDa)

9.79

Isoelectric Point (pI)

73.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LIN37 PF15306 132 - 240 4e-08 LIN37
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 47
AccI GTMKAC 1 cut(s) 150
AccII CGCG 1 cut(s) 165
AclI AACGTT 1 cut(s) 676
AclWI GGATC 1 cut(s) 5
AcoI YGGCCR 1 cut(s) 62
AcuI CTGAAG 1 cut(s) 303
AcyI GRCGYC 1 cut(s) 48
AdeI CACNNNGTG 1 cut(s) 140
AfaI GTAC 3 cut(s) 107, 251, 698
AfiI CCNNNNNNNGG 2 cut(s) 60, 121
AflIII ACRYGT 2 cut(s) 601, 631
AgsI TTSAA 3 cut(s) 380, 575, 599
AjnI CCWGG 2 cut(s) 136, 295
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AluBI AGCT 3 cut(s) 80, 410, 758
AluI AGCT 3 cut(s) 80, 410, 758
Alw21I GWGCWC 2 cut(s) 145, 661
Alw26I GTCTC 2 cut(s) 64, 498
Alw44I GTGCAC 1 cut(s) 141
AlwI GGATC 1 cut(s) 5
AoxI GGCC 3 cut(s) 62, 123, 298
ApaI GGGCCC 1 cut(s) 127
ApaLI GTGCAC 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 758
AspLEI GCGC 4 cut(s) 50, 167, 424, 687
AspS9I GGNCC 3 cut(s) 112, 123, 124
AsuHPI GGTGA 4 cut(s) 126, 491, 501, 600
AvaII GGWCC 1 cut(s) 112
BaeGI GKGCMC 2 cut(s) 127, 145
BanI GGYRCC 1 cut(s) 47
BanII GRGCYC 1 cut(s) 127
BbsI GAAGAC 3 cut(s) 309, 573, 582
Bbv12I GWGCWC 2 cut(s) 145, 661
BbvCI CCTCAGC 1 cut(s) 440
BbvI GCAGC 1 cut(s) 745
BccI CCATC 4 cut(s) 163, 184, 207, 398
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 2 cut(s) 138, 297
BcoDI GTCTC 2 cut(s) 64, 498
BfaI CTAG 3 cut(s) 645, 705, 755
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 51
BglII AGATCT 1 cut(s) 616
BisI GCNGC 5 cut(s) 51, 54, 65, 683, 759
BlsI GCNGC 5 cut(s) 52, 55, 66, 684, 760
Bme1390I CCNGG 2 cut(s) 138, 297
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 3 cut(s) 112, 123, 124
BmiI GGNNCC 3 cut(s) 49, 114, 125
BmrFI CCNGG 2 cut(s) 138, 297
BmsI GCATC 2 cut(s) 348, 472
BpiI GAAGAC 3 cut(s) 309, 573, 582
Bpu10I CCTNAGC 1 cut(s) 440
BsaHI GRCGYC 1 cut(s) 48
BsaI GGTCTC 2 cut(s) 64, 498
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 121
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 1 cut(s) 735
Bse3DI GCAATG 1 cut(s) 687
BseBI CCWGG 2 cut(s) 138, 297
BseGI GGATG 2 cut(s) 199, 589
BseLI CCNNNNNNNGG 2 cut(s) 60, 121
BseMI GCAATG 1 cut(s) 687
BseMII CTCAG 2 cut(s) 59, 431
BseNI ACTGG 1 cut(s) 735
BseRI GAGGAG 9 cut(s) 81, 201, 216, 219, 222, 225, 228, 249, 300
BseSI GKGCMC 2 cut(s) 127, 145
BseX3I CGGCCG 1 cut(s) 62
BseXI GCAGC 1 cut(s) 745
Bsh1236I CGCG 1 cut(s) 165
Bsh1285I CGRYCG 1 cut(s) 65
BshFI GGCC 3 cut(s) 64, 125, 300
BshNI GGYRCC 1 cut(s) 47
BsiEI CGRYCG 1 cut(s) 65
BsiHKAI GWGCWC 2 cut(s) 145, 661
BsiSI CCGG 1 cut(s) 45
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 2 cut(s) 60, 121
BsmAI GTCTC 2 cut(s) 64, 498
BsmFI GGGAC 1 cut(s) 98
BsnI GGCC 3 cut(s) 64, 125, 300
Bso31I GGTCTC 2 cut(s) 64, 498
Bsp120I GGGCCC 1 cut(s) 123
Bsp1286I GDGCHC 3 cut(s) 127, 145, 661
Bsp1407I TGTACA 1 cut(s) 105
Bsp143I GATC 4 cut(s) 10, 382, 616, 751
BspANI GGCC 3 cut(s) 64, 125, 300
BspCNI CTCAG 2 cut(s) 60, 432
BspFNI CGCG 1 cut(s) 165
BspLI GGNNCC 3 cut(s) 49, 114, 125
BspPI GGATC 1 cut(s) 5
BspQI GCTCTTC 1 cut(s) 720
BspT107I GGYRCC 1 cut(s) 47
BspTNI GGTCTC 2 cut(s) 64, 498
BsrDI GCAATG 1 cut(s) 687
BsrFI RCCGGY 1 cut(s) 44
BsrGI TGTACA 1 cut(s) 105
BsrI ACTGG 1 cut(s) 735
BssAI RCCGGY 1 cut(s) 44
BssMI GATC 4 cut(s) 10, 382, 616, 751
BssNI GRCGYC 1 cut(s) 48
Bst2UI CCWGG 2 cut(s) 138, 297
Bst4CI ACNGT 3 cut(s) 112, 449, 741
Bst6I CTCTTC 4 cut(s) 246, 267, 501, 720
BstACI GRCGYC 1 cut(s) 48
BstAUI TGTACA 1 cut(s) 105
BstC8I GCNNGC 2 cut(s) 163, 711
BstDEI CTNAG 2 cut(s) 68, 440
BstF5I GGATG 2 cut(s) 199, 589
BstFNI CGCG 1 cut(s) 165
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 4 cut(s) 50, 167, 424, 687
BstKTI GATC 4 cut(s) 13, 385, 619, 754
BstMAI GTCTC 2 cut(s) 64, 498
BstMBI GATC 4 cut(s) 10, 382, 616, 751
BstMCI CGRYCG 1 cut(s) 65
BstMWI GCNNNNNNNGC 2 cut(s) 47, 297
BstNI CCWGG 2 cut(s) 138, 297
BstNSI RCATGY 2 cut(s) 605, 635
BstSCI CCNGG 2 cut(s) 136, 295
BstSFI CTRYAG 1 cut(s) 6
BstSLI GKGCMC 2 cut(s) 127, 145
BstUI CGCG 1 cut(s) 165
BstV1I GCAGC 1 cut(s) 745
BstV2I GAAGAC 3 cut(s) 309, 573, 582
BstX2I RGATCY 2 cut(s) 10, 616
BstXI CCANNNNNNTGG 1 cut(s) 520
BstYI RGATCY 2 cut(s) 10, 616
BstZI CGGCCG 1 cut(s) 62
BsuRI GGCC 3 cut(s) 64, 125, 300
BtsCI GGATG 2 cut(s) 199, 589
BtsIMutI CAGTG 1 cut(s) 728
Cac8I GCNNGC 2 cut(s) 163, 711
CfoI GCGC 4 cut(s) 50, 167, 424, 687
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 3 cut(s) 112, 123, 124
CsiI ACCWGGT 1 cut(s) 136
Csp6I GTAC 3 cut(s) 106, 250, 697
CviAII CATG 4 cut(s) 221, 435, 602, 632
CviQI GTAC 3 cut(s) 106, 250, 697
DdeI CTNAG 2 cut(s) 68, 440
DinI GGCGCC 1 cut(s) 49
DpnI GATC 4 cut(s) 12, 384, 618, 753
DpnII GATC 4 cut(s) 10, 382, 616, 751
DraIII CACNNNGTG 1 cut(s) 140
EaeI YGGCCR 1 cut(s) 62
EagI CGGCCG 1 cut(s) 62
Eam1104I CTCTTC 4 cut(s) 246, 267, 501, 720
EarI CTCTTC 4 cut(s) 246, 267, 501, 720
EciI GGCGGA 2 cut(s) 255, 525
EclXI CGGCCG 1 cut(s) 62
Eco24I GRGCYC 1 cut(s) 127
Eco31I GGTCTC 2 cut(s) 64, 498
Eco47I GGWCC 1 cut(s) 112
Eco52I CGGCCG 1 cut(s) 62
Eco57I CTGAAG 1 cut(s) 303
EcoRII CCWGG 2 cut(s) 136, 295
EcoT38I GRGCYC 1 cut(s) 127
EgeI GGCGCC 1 cut(s) 49
EheI GGCGCC 1 cut(s) 49
FaeI CATG 4 cut(s) 224, 438, 605, 635
FaqI GGGAC 1 cut(s) 98
FatI CATG 4 cut(s) 220, 434, 601, 631
FauI CCCGC 2 cut(s) 91, 170
FauNDI CATATG 1 cut(s) 102
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 5 cut(s) 51, 54, 65, 683, 759
FokI GGATG 2 cut(s) 186, 596
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 5 cut(s) 51, 54, 65, 683, 759
FspBI CTAG 3 cut(s) 645, 705, 755
GlaI GCGC 4 cut(s) 49, 166, 423, 686
GluI GCNGC 5 cut(s) 51, 54, 65, 683, 759
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 3 cut(s) 64, 125, 300
HapII CCGG 1 cut(s) 45
HhaI GCGC 4 cut(s) 50, 167, 424, 687
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 4 cut(s) 224, 438, 605, 635
Hin6I GCGC 4 cut(s) 48, 165, 422, 685
HinP1I GCGC 4 cut(s) 48, 165, 422, 685
HinfI GANTC 3 cut(s) 329, 532, 590
HpaII CCGG 1 cut(s) 45
HphI GGTGA 4 cut(s) 126, 491, 501, 600
Hpy166II GTNNAC 3 cut(s) 108, 143, 151
Hpy188I TCNGA 3 cut(s) 387, 468, 746
Hpy188III TCNNGA 4 cut(s) 326, 380, 394, 455
Hpy8I GTNNAC 3 cut(s) 108, 143, 151
HpyAV CCTTC 5 cut(s) 296, 516, 538, 545, 731
HpyCH4III ACNGT 3 cut(s) 112, 449, 741
HpyCH4IV ACGT 2 cut(s) 640, 676
HpyCH4V TGCA 2 cut(s) 143, 692
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 297
HpyF3I CTNAG 2 cut(s) 68, 440
HpySE526I ACGT 2 cut(s) 640, 676
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 4 cut(s) 224, 438, 605, 635
HspAI GCGC 4 cut(s) 48, 165, 422, 685
KasI GGCGCC 1 cut(s) 47
Kzo9I GATC 4 cut(s) 10, 382, 616, 751
LguI GCTCTTC 1 cut(s) 720
LmnI GCTCC 5 cut(s) 85, 172, 229, 313, 407
Lsp1109I GCAGC 1 cut(s) 745
LweI GCATC 2 cut(s) 348, 472
MabI ACCWGGT 1 cut(s) 136
MaeI CTAG 3 cut(s) 645, 705, 755
MaeII ACGT 2 cut(s) 640, 676
MaeIII GTNAC 1 cut(s) 529
MalI GATC 4 cut(s) 12, 384, 618, 753
MboI GATC 4 cut(s) 10, 382, 616, 751
MflI RGATCY 2 cut(s) 10, 616
MhlI GDGCHC 3 cut(s) 127, 145, 661
MluCI AATT 3 cut(s) 187, 273, 766
Mly113I GGCGCC 1 cut(s) 48
MlyI GAGTC 2 cut(s) 323, 526
MseI TTAA 3 cut(s) 276, 623, 665
MslI CAYNNNNRTG 2 cut(s) 219, 518
MspA1I CMGCKG 1 cut(s) 67
MspI CCGG 1 cut(s) 45
MspR9I CCNGG 2 cut(s) 138, 297
MvaI CCWGG 2 cut(s) 138, 297
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 2 cut(s) 47, 297
NarI GGCGCC 1 cut(s) 48
NdeI CATATG 1 cut(s) 102
NdeII GATC 4 cut(s) 10, 382, 616, 751
NlaIII CATG 4 cut(s) 224, 438, 605, 635
NlaIV GGNNCC 3 cut(s) 49, 114, 125
NmuCI GTSAC 1 cut(s) 529
NspI RCATGY 2 cut(s) 605, 635
PciI ACATGT 2 cut(s) 601, 631
PciSI GCTCTTC 1 cut(s) 720
PcsI WCGNNNNNNNCGW 1 cut(s) 453
PfeI GAWTC 1 cut(s) 590
PkrI GCNGC 5 cut(s) 52, 55, 66, 684, 760
PleI GAGTC 2 cut(s) 323, 526
PluTI GGCGCC 1 cut(s) 51
PpsI GAGTC 2 cut(s) 323, 526
PscI ACATGT 2 cut(s) 601, 631
Psp1406I AACGTT 1 cut(s) 676
Psp6I CCWGG 2 cut(s) 136, 295
PspGI CCWGG 2 cut(s) 136, 295
PspN4I GGNNCC 3 cut(s) 49, 114, 125
PspOMI GGGCCC 1 cut(s) 123
PspPI GGNCC 3 cut(s) 112, 123, 124
PsuI RGATCY 2 cut(s) 10, 616
RsaI GTAC 3 cut(s) 107, 251, 698
RsaNI GTAC 3 cut(s) 106, 250, 697
RseI CAYNNNNRTG 2 cut(s) 219, 518
SapI GCTCTTC 1 cut(s) 720
SaqAI TTAA 3 cut(s) 276, 623, 665
SatI GCNGC 5 cut(s) 51, 54, 65, 683, 759
Sau3AI GATC 4 cut(s) 10, 382, 616, 751
Sau96I GGNCC 3 cut(s) 112, 123, 124
SchI GAGTC 2 cut(s) 323, 526
ScrFI CCNGG 2 cut(s) 138, 297
SduI GDGCHC 3 cut(s) 127, 145, 661
SexAI ACCWGGT 1 cut(s) 136
SfaNI GCATC 2 cut(s) 348, 472
SfcI CTRYAG 1 cut(s) 6
SfoI GGCGCC 1 cut(s) 49
SgrAI CRCCGGYG 1 cut(s) 44
SinI GGWCC 1 cut(s) 112
SmiMI CAYNNNNRTG 2 cut(s) 219, 518
Sse9I AATT 3 cut(s) 187, 273, 766
SspDI GGCGCC 1 cut(s) 47
SspMI CTAG 3 cut(s) 645, 705, 755
StyD4I CCNGG 2 cut(s) 136, 295
TaaI ACNGT 3 cut(s) 112, 449, 741
TaiI ACGT 2 cut(s) 643, 679
TaqI TCGA 1 cut(s) 655
TaqII GACCGA 1 cut(s) 420
TasI AATT 3 cut(s) 187, 273, 766
TatI WGTACW 1 cut(s) 105
TauI GCSGC 4 cut(s) 53, 56, 67, 685
TfiI GAWTC 1 cut(s) 590
Tru1I TTAA 3 cut(s) 276, 623, 665
Tru9I TTAA 3 cut(s) 276, 623, 665
TscAI CASTG 1 cut(s) 735
TseFI GTSAC 1 cut(s) 529
TseI GCWGC 1 cut(s) 758
Tsp45I GTSAC 1 cut(s) 529
TspDTI ATGAA 2 cut(s) 182, 209
TspGWI ACGGA 1 cut(s) 303
TspRI CASTG 1 cut(s) 735
VneI GTGCAC 1 cut(s) 141
VpaK11BI GGWCC 1 cut(s) 112
XceI RCATGY 2 cut(s) 605, 635
XcmI CCANNNNNNNNNTGG 1 cut(s) 134
XmiI GTMKAC 1 cut(s) 150
XspI CTAG 3 cut(s) 645, 705, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.