Rorug01G0449000

Alcohol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001983
Physical Location & Seq
Forward (+)
54493499 .. 54494488
990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0449000.1

Sequence Viewer

Length: 990 bp
ATGCTCGCCGTTCAACTCCTCCCCACCCTAAATCCCAAACCCTCCATCACCACCACCCCAAACCGCCGCACCGTCCTTAAACCCTTCCACAACCCCAAAACCCTAAGACCCAACTCCTCCCCATTCACCGTCCGCTCCGTCCTCCAATACAACAGAAAGCCCCAATTGTCCGGCGACACTCCCCGCGTGGTCGTCATCACCTCCGGCAAGGGCGGCGTCGGCAAGACCACCACCACCGCCAATGTCGGCCTCTCCCTCGCCCGCCTCGGCTTCTCCGTCGTCGCCATCGACGCCGACGTCGGCCTCCGCAACCTCGACCTCCTCCTCGGCCTCGAGAACCGCGTCAACTACACCGTCGTCGAGGTCCTCAACGGCGACTGCCGCCTCGACCAGGCTCTCGTCAGGGACAAGCGCTGGTCCAACTTCGAATTGCTCTGCATTTCCAAGCCCAGATCCAAATTGCCCATGGGCTTCGGCGGCAAAGCCCTCGTCTGGGTCGTCGACGCCCTAAAGGCCCGGCAGGAGGGCTGCCCGGACTTCATCCTCATCGACTGCCCGGCCGGCATTGATGCTGGATTCATTACCGCCATCACTCCGGCCAATGAGGCTGTGCTGGTGACCACGCCGGACATTACCAGCTTGCGAGACGCCGACAGGGTCATTGGGCTGCTGGAGTGTGATGGAATCAGAGACATTAAGATGATGGTGAACCGGGTTCGGACTGATATGATCAAAGGTGAGGACATGATGTCTGTGCTGGATGTGCAGGAGATGCTGGGATTGTCATTATTGGGGATGATTCCGGAGGACACGGAGGTGATTCGAAGCACCAACAGAGGGTACCCTCTGGTTTTGAATAGGCCACCCACATTGGCTGGACTGGCTTTCGAGCAGGCTGCATGGAGACTTGTTGAGCAGGACACTATGAAGGCTGTCATGGTTGAGGAAGAGCCCAAGAAGCGCGGCTTCTTCTCATTTTTCGGAGGGTGA

Protein Analysis

329

Amino Acids

36.09

Weight (kDa)

8.79

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ParA PF10609 62 - 300 3.2e-17 NUBPL iron-transfer P-loop NTPase
AAA_31 PF13614 62 - 215 2.1e-14 AAA domain
MipZ PF09140 62 - 213 2.3e-07 ATPase MipZ
CbiA PF01656 64 - 282 1.7e-19 CobQ/CobB/MinD/ParA nucleotide binding domain
Fer4_NifH PF00142 68 - 307 9.1e-08 4Fe-4S iron sulfur cluster binding proteins, NifH/frxC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 296
AatII GACGTC 1 cut(s) 300
Acc65I GGTACC 1 cut(s) 840
AccB1I GGYRCC 1 cut(s) 840
AccBSI CCGCTC 1 cut(s) 135
AccI GTMKAC 1 cut(s) 501
AccII CGCG 3 cut(s) 186, 342, 963
AccIII TCCGGA 1 cut(s) 802
AclWI GGATC 1 cut(s) 447
AcoI YGGCCR 2 cut(s) 558, 597
AcyI GRCGYC 5 cut(s) 216, 291, 297, 504, 648
AfaI GTAC 1 cut(s) 842
AfeI AGCGCT 1 cut(s) 413
AfiI CCNNNNNNNGG 5 cut(s) 391, 492, 493, 523, 837
AgsI TTSAA 2 cut(s) 14, 856
AhdI GACNNNNNGTC 1 cut(s) 748
AjnI CCWGG 1 cut(s) 390
AleI CACNNNNGTG 1 cut(s) 815
AluBI AGCT 1 cut(s) 639
AluI AGCT 1 cut(s) 639
Alw26I GTCTC 3 cut(s) 639, 684, 898
AlwI GGATC 1 cut(s) 447
Ama87I CYCGRG 1 cut(s) 332
Aor13HI TCCGGA 1 cut(s) 802
Aor51HI AGCGCT 1 cut(s) 413
AoxI GGCC 7 cut(s) 247, 301, 328, 513, 558, 597, 860
ApeKI GCWGC 3 cut(s) 528, 667, 896
ArsI GACNNNNNNTTYG 2 cut(s) 474, 506
Asp718I GGTACC 1 cut(s) 840
AspLEI GCGC 2 cut(s) 414, 963
AspS9I GGNCC 3 cut(s) 364, 417, 514
AsuC2I CCSGG 4 cut(s) 517, 533, 557, 713
AsuHPI GGTGA 7 cut(s) 40, 118, 190, 628, 718, 749, 829
AsuII TTCGAA 2 cut(s) 426, 823
AvaI CYCGRG 1 cut(s) 332
AvaII GGWCC 2 cut(s) 364, 417
BanI GGYRCC 1 cut(s) 840
BanII GRGCYC 1 cut(s) 954
BbvI GCAGC 3 cut(s) 515, 654, 883
BccI CCATC 5 cut(s) 53, 293, 596, 674, 697
BceAI ACGGC 1 cut(s) 388
BcgI CGANNNNNNTGC 4 cut(s) 469, 503, 878, 912
BciT130I CCWGG 1 cut(s) 392
BclI TGATCA 1 cut(s) 729
BcnI CCSGG 4 cut(s) 517, 533, 557, 713
BcoDI GTCTC 3 cut(s) 639, 684, 898
BfoI RGCGCY 1 cut(s) 415
BglI GCCNNNNNGGC 3 cut(s) 512, 561, 605
BisI GCNGC 8 cut(s) 67, 214, 382, 478, 529, 668, 897, 964
BlsI GCNGC 8 cut(s) 68, 215, 383, 479, 530, 669, 898, 965
Bme1390I CCNGG 5 cut(s) 392, 517, 533, 557, 713
Bme18I GGWCC 2 cut(s) 364, 417
BmeRI GACNNNNNGTC 1 cut(s) 748
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 3 cut(s) 364, 417, 514
BmiI GGNNCC 1 cut(s) 842
BmrFI CCNGG 5 cut(s) 392, 517, 533, 557, 713
BmsI GCATC 2 cut(s) 559, 762
BpmI CTGGAG 1 cut(s) 692
Bpu14I TTCGAA 2 cut(s) 426, 823
BpuMI CCSGG 4 cut(s) 517, 533, 557, 713
BsaHI GRCGYC 5 cut(s) 216, 291, 297, 504, 648
BsaJI CCNNGG 3 cut(s) 265, 325, 465
BsaWI WCCGGW 1 cut(s) 802
Bsc4I CCNNNNNNNGG 5 cut(s) 391, 492, 493, 523, 837
Bse118I RCCGGY 1 cut(s) 560
Bse1I ACTGG 1 cut(s) 885
BseAI TCCGGA 1 cut(s) 802
BseBI CCWGG 1 cut(s) 392
BseDI CCNNGG 3 cut(s) 265, 325, 465
BseGI GGATG 3 cut(s) 540, 766, 801
BseLI CCNNNNNNNGG 5 cut(s) 391, 492, 493, 523, 837
BseNI ACTGG 1 cut(s) 885
BseRI GAGGAG 4 cut(s) 8, 106, 311, 314
BseX3I CGGCCG 1 cut(s) 558
BseXI GCAGC 3 cut(s) 515, 654, 883
BseYI CCCAGC 1 cut(s) 775
BsgI GTGCAG 1 cut(s) 785
Bsh1236I CGCG 3 cut(s) 186, 342, 963
Bsh1285I CGRYCG 1 cut(s) 561
BshFI GGCC 7 cut(s) 249, 303, 330, 515, 560, 599, 862
BshNI GGYRCC 1 cut(s) 840
BsiEI CGRYCG 1 cut(s) 561
BsiHKCI CYCGRG 1 cut(s) 332
BslFI GGGAC 1 cut(s) 419
BslI CCNNNNNNNGG 5 cut(s) 391, 492, 493, 523, 837
BsmAI GTCTC 3 cut(s) 639, 684, 898
BsmBI CGTCTC 1 cut(s) 639
BsmFI GGGAC 1 cut(s) 419
BsnI GGCC 7 cut(s) 249, 303, 330, 515, 560, 599, 862
BsoBI CYCGRG 1 cut(s) 332
Bsp119I TTCGAA 2 cut(s) 426, 823
Bsp1286I GDGCHC 1 cut(s) 954
Bsp13I TCCGGA 1 cut(s) 802
Bsp143I GATC 2 cut(s) 452, 729
Bsp19I CCATGG 1 cut(s) 465
BspANI GGCC 7 cut(s) 249, 303, 330, 515, 560, 599, 862
BspEI TCCGGA 1 cut(s) 802
BspFNI CGCG 3 cut(s) 186, 342, 963
BspLI GGNNCC 1 cut(s) 842
BspPI GGATC 1 cut(s) 447
BspQI GCTCTTC 1 cut(s) 942
BspT104I TTCGAA 2 cut(s) 426, 823
BspT107I GGYRCC 1 cut(s) 840
BsrBI CCGCTC 1 cut(s) 135
BsrFI RCCGGY 1 cut(s) 560
BsrI ACTGG 1 cut(s) 885
BssAI RCCGGY 1 cut(s) 560
BssECI CCNNGG 3 cut(s) 265, 325, 465
BssMI GATC 2 cut(s) 452, 729
BssNI GRCGYC 5 cut(s) 216, 291, 297, 504, 648
BssT1I CCWWGG 1 cut(s) 465
Bst2UI CCWGG 1 cut(s) 392
Bst4CI ACNGT 3 cut(s) 73, 130, 355
Bst6I CTCTTC 1 cut(s) 942
BstACI GRCGYC 5 cut(s) 216, 291, 297, 504, 648
BstAPI GCANNNNNTGC 1 cut(s) 772
BstBI TTCGAA 2 cut(s) 426, 823
BstC8I GCNNGC 5 cut(s) 6, 262, 562, 641, 894
BstDEI CTNAG 1 cut(s) 104
BstDSI CCRYGG 1 cut(s) 465
BstEII GGTNACC 1 cut(s) 616
BstENI CCTNNNNNAGG 1 cut(s) 389
BstF5I GGATG 3 cut(s) 540, 766, 801
BstFNI CGCG 3 cut(s) 186, 342, 963
BstH2I RGCGCY 1 cut(s) 415
BstHHI GCGC 2 cut(s) 414, 963
BstKTI GATC 2 cut(s) 455, 732
BstMAI GTCTC 3 cut(s) 639, 684, 898
BstMBI GATC 2 cut(s) 452, 729
BstMCI CGRYCG 1 cut(s) 561
BstNI CCWGG 1 cut(s) 392
BstPI GGTNACC 1 cut(s) 616
BstSCI CCNGG 5 cut(s) 390, 515, 531, 555, 711
BstUI CGCG 3 cut(s) 186, 342, 963
BstV1I GCAGC 3 cut(s) 515, 654, 883
BstX2I RGATCY 1 cut(s) 452
BstYI RGATCY 1 cut(s) 452
BstZI CGGCCG 1 cut(s) 558
BsuRI GGCC 7 cut(s) 249, 303, 330, 515, 560, 599, 862
BtgI CCRYGG 1 cut(s) 465
BtsCI GGATG 3 cut(s) 540, 766, 801
Cac8I GCNNGC 5 cut(s) 6, 262, 562, 641, 894
CfoI GCGC 2 cut(s) 414, 963
Cfr10I RCCGGY 1 cut(s) 560
Cfr13I GGNCC 3 cut(s) 364, 417, 514
CseI GACGC 5 cut(s) 205, 299, 331, 512, 656
Csp6I GTAC 1 cut(s) 841
CspCI CAANNNNNGTGG 2 cut(s) 852, 887
CviAII CATG 4 cut(s) 466, 745, 900, 937
CviQI GTAC 1 cut(s) 841
DdeI CTNAG 1 cut(s) 104
DpnI GATC 2 cut(s) 454, 731
DpnII GATC 2 cut(s) 452, 729
DrdI GACNNNNNNGTC 1 cut(s) 296
DriI GACNNNNNGTC 1 cut(s) 748
DseDI GACNNNNNNGTC 1 cut(s) 296
EaeI YGGCCR 2 cut(s) 558, 597
EagI CGGCCG 1 cut(s) 558
Eam1104I CTCTTC 1 cut(s) 942
Eam1105I GACNNNNNGTC 1 cut(s) 748
EarI CTCTTC 1 cut(s) 942
EclXI CGGCCG 1 cut(s) 558
Eco130I CCWWGG 1 cut(s) 465
Eco24I GRGCYC 1 cut(s) 954
Eco47I GGWCC 2 cut(s) 364, 417
Eco47III AGCGCT 1 cut(s) 413
Eco52I CGGCCG 1 cut(s) 558
Eco88I CYCGRG 1 cut(s) 332
Eco91I GGTNACC 1 cut(s) 616
EcoNI CCTNNNNNAGG 1 cut(s) 389
EcoO109I RGGNCCY 1 cut(s) 364
EcoO65I GGTNACC 1 cut(s) 616
EcoRII CCWGG 1 cut(s) 390
EcoT14I CCWWGG 1 cut(s) 465
EcoT38I GRGCYC 1 cut(s) 954
ErhI CCWWGG 1 cut(s) 465
Esp3I CGTCTC 1 cut(s) 639
FaeI CATG 4 cut(s) 469, 748, 903, 940
FaiI YATR 6 cut(s) 467, 728, 746, 901, 926, 938
FalI AAGNNNNNCTT 2 cut(s) 950, 982
FaqI GGGAC 1 cut(s) 419
FatI CATG 4 cut(s) 465, 744, 899, 936
FauI CCCGC 2 cut(s) 191, 269
FbaI TGATCA 1 cut(s) 729
FblI GTMKAC 1 cut(s) 501
Fnu4HI GCNGC 8 cut(s) 67, 214, 382, 478, 529, 668, 897, 964
FokI GGATG 3 cut(s) 527, 773, 808
FriOI GRGCYC 1 cut(s) 954
Fsp4HI GCNGC 8 cut(s) 67, 214, 382, 478, 529, 668, 897, 964
GlaI GCGC 2 cut(s) 413, 962
GluI GCNGC 8 cut(s) 67, 214, 382, 478, 529, 668, 897, 964
GsaI CCCAGC 1 cut(s) 779
GsuI CTGGAG 1 cut(s) 692
HaeII RGCGCY 1 cut(s) 415
HaeIII GGCC 7 cut(s) 249, 303, 330, 515, 560, 599, 862
HgaI GACGC 5 cut(s) 205, 299, 331, 512, 656
HhaI GCGC 2 cut(s) 414, 963
Hin1I GRCGYC 5 cut(s) 216, 291, 297, 504, 648
Hin1II CATG 4 cut(s) 469, 748, 903, 940
Hin6I GCGC 2 cut(s) 412, 961
HinP1I GCGC 2 cut(s) 412, 961
HincII GTYRAC 2 cut(s) 346, 502
HindII GTYRAC 2 cut(s) 346, 502
HinfI GANTC 4 cut(s) 576, 684, 799, 820
HphI GGTGA 7 cut(s) 40, 118, 190, 628, 718, 749, 829
Hpy166II GTNNAC 3 cut(s) 346, 502, 709
Hpy188I TCNGA 3 cut(s) 689, 720, 983
Hpy188III TCNNGA 2 cut(s) 334, 803
Hpy8I GTNNAC 3 cut(s) 346, 502, 709
HpyAV CCTTC 2 cut(s) 94, 922
HpyCH4III ACNGT 3 cut(s) 73, 130, 355
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 3 cut(s) 438, 766, 899
HpyF3I CTNAG 1 cut(s) 104
HpySE526I ACGT 1 cut(s) 297
Hsp92I GRCGYC 5 cut(s) 216, 291, 297, 504, 648
Hsp92II CATG 4 cut(s) 469, 748, 903, 940
HspAI GCGC 2 cut(s) 412, 961
Kpn2I TCCGGA 1 cut(s) 802
KpnI GGTACC 1 cut(s) 844
KroI GCCGGC 1 cut(s) 560
KroNI GCCGGC 1 cut(s) 562
Ksp22I TGATCA 1 cut(s) 729
Kzo9I GATC 2 cut(s) 452, 729
LguI GCTCTTC 1 cut(s) 942
LmnI GCTCC 1 cut(s) 140
Lsp1109I GCAGC 3 cut(s) 515, 654, 883
LweI GCATC 2 cut(s) 559, 762
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 1 cut(s) 616
MalI GATC 2 cut(s) 454, 731
MbiI CCGCTC 1 cut(s) 135
MboI GATC 2 cut(s) 452, 729
MboII GAAGA 2 cut(s) 959, 961
MfeI CAATTG 1 cut(s) 164
MflI RGATCY 1 cut(s) 452
MhlI GDGCHC 1 cut(s) 954
MluCI AATT 3 cut(s) 164, 428, 458
MmeI TCCRAC 1 cut(s) 444
MroI TCCGGA 1 cut(s) 802
MroNI GCCGGC 1 cut(s) 560
MseI TTAA 2 cut(s) 78, 696
MslI CAYNNNNRTG 2 cut(s) 698, 815
MspR9I CCNGG 5 cut(s) 392, 517, 533, 557, 713
MunI CAATTG 1 cut(s) 164
MvaI CCWGG 1 cut(s) 392
MvnI CGCG 3 cut(s) 186, 342, 963
NaeI GCCGGC 1 cut(s) 562
NciI CCSGG 4 cut(s) 517, 533, 557, 713
NcoI CCATGG 1 cut(s) 465
NdeII GATC 2 cut(s) 452, 729
NgoMIV GCCGGC 1 cut(s) 560
NlaIII CATG 4 cut(s) 469, 748, 903, 940
NlaIV GGNNCC 1 cut(s) 842
NmeAIII GCCGAG 2 cut(s) 246, 306
NmuCI GTSAC 1 cut(s) 616
NspV TTCGAA 2 cut(s) 426, 823
OliI CACNNNNGTG 1 cut(s) 815
PaeR7I CTCGAG 1 cut(s) 332
PciSI GCTCTTC 1 cut(s) 942
PcsI WCGNNNNNNNCGW 5 cut(s) 273, 285, 294, 339, 495
PdiI GCCGGC 1 cut(s) 562
PfeI GAWTC 4 cut(s) 576, 684, 799, 820
PflFI GACNNNGTC 1 cut(s) 656
PkrI GCNGC 8 cut(s) 68, 215, 383, 479, 530, 669, 898, 965
PpuMI RGGWCCY 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 364
Psp6I CCWGG 1 cut(s) 390
PspEI GGTNACC 1 cut(s) 616
PspFI CCCAGC 1 cut(s) 775
PspGI CCWGG 1 cut(s) 390
PspN4I GGNNCC 1 cut(s) 842
PspPI GGNCC 3 cut(s) 364, 417, 514
PspPPI RGGWCCY 1 cut(s) 364
PsuI RGATCY 1 cut(s) 452
PsyI GACNNNGTC 1 cut(s) 656
RsaI GTAC 1 cut(s) 842
RsaNI GTAC 1 cut(s) 841
RseI CAYNNNNRTG 2 cut(s) 698, 815
SalI GTCGAC 1 cut(s) 500
SapI GCTCTTC 1 cut(s) 942
SaqAI TTAA 2 cut(s) 78, 696
SatI GCNGC 8 cut(s) 67, 214, 382, 478, 529, 668, 897, 964
Sau3AI GATC 2 cut(s) 452, 729
Sau96I GGNCC 3 cut(s) 364, 417, 514
ScrFI CCNGG 5 cut(s) 392, 517, 533, 557, 713
SduI GDGCHC 1 cut(s) 954
SetI ASST 8 cut(s) 203, 300, 315, 321, 366, 641, 739, 819
SfaNI GCATC 2 cut(s) 559, 762
Sfr274I CTCGAG 1 cut(s) 332
SfuI TTCGAA 2 cut(s) 426, 823
SgrDI CGTCGACG 1 cut(s) 500
SinI GGWCC 2 cut(s) 364, 417
SlaI CTCGAG 1 cut(s) 332
SmiMI CAYNNNNRTG 2 cut(s) 698, 815
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 3 cut(s) 164, 428, 458
StyD4I CCNGG 5 cut(s) 390, 515, 531, 555, 711
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 3 cut(s) 73, 130, 355
TaiI ACGT 1 cut(s) 300
TasI AATT 3 cut(s) 164, 428, 458
TauI GCSGC 5 cut(s) 69, 216, 384, 480, 966
TfiI GAWTC 4 cut(s) 576, 684, 799, 820
Tru1I TTAA 2 cut(s) 78, 696
Tru9I TTAA 2 cut(s) 78, 696
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 3 cut(s) 528, 667, 896
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 3 cut(s) 529, 568, 941
TspGWI ACGGA 3 cut(s) 127, 265, 827
Tth111I GACNNNGTC 1 cut(s) 656
VpaK11BI GGWCC 2 cut(s) 364, 417
XagI CCTNNNNNAGG 1 cut(s) 389
XcmI CCANNNNNNNNNTGG 1 cut(s) 463
XhoI CTCGAG 1 cut(s) 332
XmiI GTMKAC 1 cut(s) 501
ZraI GACGTC 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.