Rorug04G0062100

Alcohol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001983
Physical Location & Seq
Reverse (-)
9718903 .. 9723408
4506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0062100.1

Sequence Viewer

Length: 1734 bp
ATGGCGTTCAGCATCACACACCAGATCGGAGCACTGGCGGGCACGCCGATATCGGTGACGGAGCCGACCACGGCCTCCGGCGAATCGACGGCGAGCGTGAGCGCGTCCGCGGTGAGGAAGTCACCGGCGACGACGAGCCTGACGTGCAAAATTCAGAAGCCGGAGGTGAAGGACGGGATGCTAACCCCGCCGTTGAGCCCGTGCCGGTCGCCGGTTCTCGGGGCGACCCGGCCGGATCTGTCGGTGGCGTGCCAGGCGTACGCGGCGGAGGCGGAGGCTCCGGTTTTGGAGCACGAGGCGAGGGCCGCCGGGGAGAGGATAAACGGCGTGCCGGTTTTTGTAATGATGCCGTTGGACAGCGTGACGATGAACCACACGGTGAATCGGAAGAAGGCGATGAACGCGAGTCTTCAGGCGTTGAAGAGCGCAGGGGTGGAGGGGATCATGATGGACGTGTGGTGGGGGTTGGTGGAGAGGGACGCGCCCGGCGCGTACAATTGGGGCGGTTACGCCGAGCTCTTGGAGATGGCCAAGAAACACGGCCTCAAAGTTCAGGCCGTCATGTCGTACCACCAGTGTGGCGGTAACGTCGGGGACTCTTGCACTATTCCTTTACCCAGTTGGGTTGTGGAGGAGGTTAACAACGATCCAGACCTTACATACACTGATCAATGGGGAAATAGGAACCACGAGTATCTTTCACTTGGCGCTGATACACTTCCGGTCCTTAAAGGCCGCACGCCAGTGCAATGTTACGCTGACTTCATGCGTGCCTTCAGAGATAACTTCAAACACCTTCTTGGTGACACCATTGTGGAAATCCAAGTTGGAATGGGTCCAGCAGGTGAGCTCCGTTACCCTTCATATCCAGAGGGGGATGGGAGATGGTCATTTCCTGGAATTGGTGGCTTCCAATGTTTTGACAAGTATATGCTGAGTAGCTTAAAAGCTTATTGTGAATCTAAGGGTAAGCCAGAATGGGGTAGCACAGGCCCCACTGATGCCGGTCACTACAATAACTGGCCGGAAGATACCCCCTTCTTCAGAAGAGAAGGTGGAGGCTGGAATAGTAGTTACGGTGAATTCTTCCTCGGCTGGTACTCCCAGTTGCTCATGGACCATGGTGAGAGGATCCTTTCCTCAGCCAAGTCTATCTATGAGAATGAGGGCGTGAAAATCTCAGTAAAGATTGCAGGCATCCATTGGCATTATGGAACCCGGTCCCACGCCCCCGAGCTCACAGCAGGGTACTACAACACACGATTCCGGGATGGTTACCTACCCATTGCTCAGATGTTAGCTCGCCATGGTGCCATTTTCAATTTCACTTGCATCGAGATGCACGACCACGAGCAGCCACAGGACGCCCAATGTTCCCCTGAAAAGCTTGTTAGGCAAGTGGCCTTGGCCACTCAGAAGGCAGGGGTACCACTTGCAGGTGAAAATGCATTGCCAAGGTATGATGACTATGCACACGAGCAGATCTTGCAAGCATCAGCGTTGAACATTGAGGGCAACACTGAAGGCAAGCAGATGTGCGCGTTCACATACTTGAGGATGAACCCGCAACTATTCCAGGCCGACAACTGGAGGCGTTTTGTGTCATTTGTGAAGAAAATGAAGGAAGGAAAGAACACTCAAAAGTGTAGGGAACAGGTGGAGAGGGAAGCCGAGCATTTCTTGCACATTACAACACCTTTGGTGCAGGAGGCTGCTGTTGCTCTGACCCACTGA

Protein Analysis

577

Amino Acids

63.8

Weight (kDa)

6.04

Isoelectric Point (pI)

38.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 113 - 536 3.1e-173 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 833, 1427
Acc36I ACCTGC 2 cut(s) 833, 1427
Acc65I GGTACC 1 cut(s) 1426
AccB1I GGYRCC 2 cut(s) 1310, 1426
AccII CGCG 7 cut(s) 104, 110, 263, 404, 482, 491, 1541
AclWI GGATC 5 cut(s) 243, 449, 641, 1126, 1139
AcoI YGGCCR 4 cut(s) 230, 528, 1022, 1407
AcsI RAATTY 2 cut(s) 150, 1082
AcuI CTGAAG 4 cut(s) 395, 760, 1027, 1542
AcyI GRCGYC 1 cut(s) 1365
AdeI CACNNNGTG 1 cut(s) 379
AfaI GTAC 6 cut(s) 260, 494, 569, 1100, 1250, 1428
AfiI CCNNNNNNNGG 8 cut(s) 114, 211, 218, 315, 902, 980, 1436, 1587
AflIII ACRYGT 1 cut(s) 453
AgsI TTSAA 4 cut(s) 421, 790, 1321, 1504
AjiI CACGTC 2 cut(s) 144, 454
AjnI CCWGG 3 cut(s) 252, 895, 1575
AjuI GAANNNNNNNTTGG 2 cut(s) 810, 842
AleI CACNNNNGTG 3 cut(s) 576, 743, 812
AluBI AGCT 7 cut(s) 517, 850, 942, 950, 1237, 1301, 1387
AluI AGCT 7 cut(s) 517, 850, 942, 950, 1237, 1301, 1387
Alw21I GWGCWC 5 cut(s) 34, 294, 519, 852, 1239
AlwI GGATC 5 cut(s) 243, 449, 641, 1126, 1139
Ama87I CYCGRG 2 cut(s) 218, 1232
ApeKI GCWGC 2 cut(s) 1354, 1712
ApoI RAATTY 2 cut(s) 150, 1082
Asp718I GGTACC 1 cut(s) 1426
AspLEI GCGC 6 cut(s) 104, 428, 484, 491, 710, 1541
AspS9I GGNCC 6 cut(s) 303, 724, 836, 992, 1117, 1221
AsuC2I CCSGG 5 cut(s) 229, 310, 486, 1219, 1268
AvaI CYCGRG 2 cut(s) 218, 1232
AvaII GGWCC 4 cut(s) 724, 836, 1117, 1221
BaeGI GKGCMC 1 cut(s) 44
BalI TGGCCA 2 cut(s) 530, 1409
BamHI GGATCC 1 cut(s) 1131
BanI GGYRCC 2 cut(s) 1310, 1426
BanII GRGCYC 4 cut(s) 200, 519, 852, 1239
BauI CACGAG 4 cut(s) 293, 689, 1349, 1475
BbsI GAAGAC 1 cut(s) 401
Bbv12I GWGCWC 5 cut(s) 34, 294, 519, 852, 1239
BbvCI CCTCAGC 1 cut(s) 1141
BbvI GCAGC 2 cut(s) 1366, 1699
BccI CCATC 5 cut(s) 442, 520, 872, 879, 1265
BceAI ACGGC 7 cut(s) 87, 105, 175, 334, 340, 542, 556
BciT130I CCWGG 3 cut(s) 254, 897, 1577
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 5 cut(s) 229, 310, 486, 1219, 1268
BfoI RGCGCY 1 cut(s) 711
BfuAI ACCTGC 2 cut(s) 833, 1427
BglII AGATCT 1 cut(s) 1482
BisI GCNGC 5 cut(s) 264, 306, 736, 1355, 1713
BlsI GCNGC 5 cut(s) 265, 307, 737, 1356, 1714
Bme1390I CCNGG 8 cut(s) 229, 254, 310, 486, 897, 1219, 1268, 1577
Bme18I GGWCC 4 cut(s) 724, 836, 1117, 1221
BmeT110I CYCGRG 2 cut(s) 218, 1232
BmgBI CACGTC 2 cut(s) 144, 454
BmgT120I GGNCC 6 cut(s) 303, 724, 836, 992, 1117, 1221
BmrFI CCNGG 8 cut(s) 229, 254, 310, 486, 897, 1219, 1268, 1577
BmrI ACTGGG 2 cut(s) 612, 1099
BmsI GCATC 8 cut(s) 21, 168, 336, 991, 1206, 1329, 1341, 1502
BmuI ACTGGG 2 cut(s) 612, 1099
BpiI GAAGAC 1 cut(s) 401
BpmI CTGGAG 1 cut(s) 1609
Bpu10I CCTNAGC 1 cut(s) 1141
BpuEI CTTGAG 1 cut(s) 1573
BpuMI CCSGG 5 cut(s) 229, 310, 486, 1219, 1268
BsaHI GRCGYC 1 cut(s) 1365
BsaJI CCNNGG 8 cut(s) 69, 108, 309, 1090, 1120, 1306, 1404, 1454
BsaWI WCCGGW 2 cut(s) 280, 721
Bsc4I CCNNNNNNNGG 8 cut(s) 114, 211, 218, 315, 902, 980, 1436, 1587
Bse118I RCCGGY 5 cut(s) 124, 204, 211, 331, 1004
Bse1I ACTGG 7 cut(s) 39, 574, 618, 743, 1025, 1105, 1592
Bse3DI GCAATG 3 cut(s) 755, 1284, 1448
BseBI CCWGG 3 cut(s) 254, 897, 1577
BseDI CCNNGG 8 cut(s) 69, 108, 309, 1090, 1120, 1306, 1404, 1454
BseGI GGATG 5 cut(s) 183, 883, 1197, 1276, 1563
BseLI CCNNNNNNNGG 8 cut(s) 114, 211, 218, 315, 902, 980, 1436, 1587
BseMI GCAATG 3 cut(s) 755, 1284, 1448
BseMII CTCAG 5 cut(s) 926, 1155, 1194, 1304, 1427
BseNI ACTGG 7 cut(s) 39, 574, 618, 743, 1025, 1105, 1592
BseRI GAGGAG 1 cut(s) 647
BseSI GKGCMC 1 cut(s) 44
BseX3I CGGCCG 1 cut(s) 230
BseXI GCAGC 2 cut(s) 1366, 1699
BsgI GTGCAG 1 cut(s) 1724
Bsh1236I CGCG 7 cut(s) 104, 110, 263, 404, 482, 491, 1541
Bsh1285I CGRYCG 2 cut(s) 209, 233
BshNI GGYRCC 2 cut(s) 1310, 1426
BsiEI CGRYCG 2 cut(s) 209, 233
BsiHKAI GWGCWC 5 cut(s) 34, 294, 519, 852, 1239
BsiHKCI CYCGRG 2 cut(s) 218, 1232
BsiWI CGTACG 1 cut(s) 258
BslFI GGGAC 3 cut(s) 491, 608, 1207
BslI CCNNNNNNNGG 8 cut(s) 114, 211, 218, 315, 902, 980, 1436, 1587
BsmFI GGGAC 3 cut(s) 491, 608, 1207
BsoBI CYCGRG 2 cut(s) 218, 1232
Bsp1286I GDGCHC 7 cut(s) 34, 44, 200, 294, 519, 852, 1239
Bsp143I GATC 7 cut(s) 24, 235, 441, 646, 667, 1131, 1482
Bsp19I CCATGG 2 cut(s) 1120, 1306
BspCNI CTCAG 5 cut(s) 927, 1154, 1193, 1303, 1426
BspFNI CGCG 7 cut(s) 104, 110, 263, 404, 482, 491, 1541
BspHI TCATGA 1 cut(s) 444
BspMI ACCTGC 2 cut(s) 833, 1427
BspPI GGATC 5 cut(s) 243, 449, 641, 1126, 1139
BspQI GCTCTTC 1 cut(s) 416
BspT107I GGYRCC 2 cut(s) 1310, 1426
BsrDI GCAATG 3 cut(s) 755, 1284, 1448
BsrFI RCCGGY 5 cut(s) 124, 204, 211, 331, 1004
BsrI ACTGG 7 cut(s) 39, 574, 618, 743, 1025, 1105, 1592
BssAI RCCGGY 5 cut(s) 124, 204, 211, 331, 1004
BssECI CCNNGG 8 cut(s) 69, 108, 309, 1090, 1120, 1306, 1404, 1454
BssMI GATC 7 cut(s) 24, 235, 441, 646, 667, 1131, 1482
BssNI GRCGYC 1 cut(s) 1365
BssSI CACGAG 4 cut(s) 293, 689, 1349, 1475
BssT1I CCWWGG 4 cut(s) 1120, 1306, 1404, 1454
Bst2BI CACGAG 4 cut(s) 293, 689, 1349, 1475
Bst2UI CCWGG 3 cut(s) 254, 897, 1577
Bst4CI ACNGT 2 cut(s) 379, 1079
Bst6I CTCTTC 2 cut(s) 416, 1042
BstACI GRCGYC 1 cut(s) 1365
BstAPI GCANNNNNTGC 2 cut(s) 1486, 1681
BstDEI CTNAG 6 cut(s) 935, 963, 1141, 1180, 1290, 1413
BstDSI CCRYGG 4 cut(s) 69, 108, 1120, 1306
BstEII GGTNACC 1 cut(s) 1274
BstF5I GGATG 5 cut(s) 183, 883, 1197, 1276, 1563
BstFNI CGCG 7 cut(s) 104, 110, 263, 404, 482, 491, 1541
BstH2I RGCGCY 1 cut(s) 711
BstHHI GCGC 6 cut(s) 104, 428, 484, 491, 710, 1541
BstKTI GATC 7 cut(s) 27, 238, 444, 649, 670, 1134, 1485
BstMBI GATC 7 cut(s) 24, 235, 441, 646, 667, 1131, 1482
BstMCI CGRYCG 2 cut(s) 209, 233
BstNI CCWGG 3 cut(s) 254, 897, 1577
BstPI GGTNACC 1 cut(s) 1274
BstSCI CCNGG 8 cut(s) 227, 252, 308, 484, 895, 1217, 1266, 1575
BstSLI GKGCMC 1 cut(s) 44
BstUI CGCG 7 cut(s) 104, 110, 263, 404, 482, 491, 1541
BstV1I GCAGC 2 cut(s) 1366, 1699
BstV2I GAAGAC 1 cut(s) 401
BstX2I RGATCY 3 cut(s) 235, 1131, 1482
BstXI CCANNNNNNTGG 1 cut(s) 578
BstYI RGATCY 3 cut(s) 235, 1131, 1482
BstZI CGGCCG 1 cut(s) 230
BtgI CCRYGG 4 cut(s) 69, 108, 1120, 1306
BtgZI GCGATG 1 cut(s) 410
BtrI CACGTC 2 cut(s) 144, 454
BtsCI GGATG 5 cut(s) 183, 883, 1197, 1276, 1563
BtsIMutI CAGTG 7 cut(s) 32, 581, 663, 750, 996, 1518, 1729
BveI ACCTGC 2 cut(s) 833, 1427
CciI TCATGA 1 cut(s) 444
CfoI GCGC 6 cut(s) 104, 428, 484, 491, 710, 1541
Cfr10I RCCGGY 5 cut(s) 124, 204, 211, 331, 1004
Cfr13I GGNCC 6 cut(s) 303, 724, 836, 992, 1117, 1221
Cfr42I CCGCGG 1 cut(s) 111
CseI GACGC 3 cut(s) 93, 488, 1373
Csp6I GTAC 6 cut(s) 259, 493, 568, 1099, 1249, 1427
CviAII CATG 6 cut(s) 445, 562, 766, 1114, 1121, 1307
CviQI GTAC 6 cut(s) 259, 493, 568, 1099, 1249, 1427
DdeI CTNAG 6 cut(s) 935, 963, 1141, 1180, 1290, 1413
DpnI GATC 7 cut(s) 26, 237, 443, 648, 669, 1133, 1484
DpnII GATC 7 cut(s) 24, 235, 441, 646, 667, 1131, 1482
DraIII CACNNNGTG 1 cut(s) 379
EaeI YGGCCR 4 cut(s) 230, 528, 1022, 1407
EagI CGGCCG 1 cut(s) 230
Eam1104I CTCTTC 2 cut(s) 416, 1042
EarI CTCTTC 2 cut(s) 416, 1042
EciI GGCGGA 2 cut(s) 281, 287
Ecl136II GAGCTC 3 cut(s) 517, 850, 1237
EclXI CGGCCG 1 cut(s) 230
Eco130I CCWWGG 4 cut(s) 1120, 1306, 1404, 1454
Eco24I GRGCYC 4 cut(s) 200, 519, 852, 1239
Eco32I GATATC 1 cut(s) 51
Eco47I GGWCC 4 cut(s) 724, 836, 1117, 1221
Eco52I CGGCCG 1 cut(s) 230
Eco53kI GAGCTC 3 cut(s) 517, 850, 1237
Eco57I CTGAAG 4 cut(s) 395, 760, 1027, 1542
Eco88I CYCGRG 2 cut(s) 218, 1232
Eco91I GGTNACC 1 cut(s) 1274
EcoICRI GAGCTC 3 cut(s) 517, 850, 1237
EcoO109I RGGNCCY 1 cut(s) 992
EcoO65I GGTNACC 1 cut(s) 1274
EcoRI GAATTC 1 cut(s) 1082
EcoRII CCWGG 3 cut(s) 252, 895, 1575
EcoRV GATATC 1 cut(s) 51
EcoT14I CCWWGG 4 cut(s) 1120, 1306, 1404, 1454
EcoT22I ATGCAT 1 cut(s) 1450
EcoT38I GRGCYC 4 cut(s) 200, 519, 852, 1239
ErhI CCWWGG 4 cut(s) 1120, 1306, 1404, 1454
FaeI CATG 6 cut(s) 448, 565, 769, 1117, 1124, 1310
FaqI GGGAC 3 cut(s) 491, 608, 1207
FatI CATG 6 cut(s) 444, 561, 765, 1113, 1120, 1306
FauI CCCGC 3 cut(s) 31, 195, 1572
FbaI TGATCA 1 cut(s) 667
Fnu4HI GCNGC 5 cut(s) 264, 306, 736, 1355, 1713
FokI GGATG 5 cut(s) 190, 890, 1184, 1283, 1570
FriOI GRGCYC 4 cut(s) 200, 519, 852, 1239
Fsp4HI GCNGC 5 cut(s) 264, 306, 736, 1355, 1713
GlaI GCGC 6 cut(s) 103, 427, 483, 490, 709, 1540
GluI GCNGC 5 cut(s) 264, 306, 736, 1355, 1713
GsuI CTGGAG 1 cut(s) 1609
HaeII RGCGCY 1 cut(s) 711
HgaI GACGC 3 cut(s) 93, 488, 1373
HhaI GCGC 6 cut(s) 104, 428, 484, 491, 710, 1541
Hin1I GRCGYC 1 cut(s) 1365
Hin1II CATG 6 cut(s) 448, 565, 769, 1117, 1124, 1310
Hin6I GCGC 6 cut(s) 102, 426, 482, 489, 708, 1539
HinP1I GCGC 6 cut(s) 102, 426, 482, 489, 708, 1539
HincII GTYRAC 1 cut(s) 640
HindII GTYRAC 1 cut(s) 640
HindIII AAGCTT 2 cut(s) 948, 1385
HinfI GANTC 6 cut(s) 83, 382, 406, 596, 959, 1263
HpaI GTTAAC 1 cut(s) 640
Hpy166II GTNNAC 2 cut(s) 640, 1545
Hpy188I TCNGA 8 cut(s) 29, 156, 387, 779, 1046, 1293, 1416, 1725
Hpy188III TCNNGA 4 cut(s) 445, 650, 869, 1336
Hpy8I GTNNAC 2 cut(s) 640, 1545
Hpy99I CGWCG 4 cut(s) 91, 133, 136, 593
HpyCH4III ACNGT 2 cut(s) 379, 1079
HpyCH4IV ACGT 3 cut(s) 143, 453, 588
HpyF3I CTNAG 6 cut(s) 935, 963, 1141, 1180, 1290, 1413
HpySE526I ACGT 3 cut(s) 143, 453, 588
Hsp92I GRCGYC 1 cut(s) 1365
Hsp92II CATG 6 cut(s) 448, 565, 769, 1117, 1124, 1310
HspAI GCGC 6 cut(s) 102, 426, 482, 489, 708, 1539
KpnI GGTACC 1 cut(s) 1430
Ksp22I TGATCA 1 cut(s) 667
KspAI GTTAAC 1 cut(s) 640
KspI CCGCGG 1 cut(s) 111
Kzo9I GATC 7 cut(s) 24, 235, 441, 646, 667, 1131, 1482
LguI GCTCTTC 1 cut(s) 416
LmnI GCTCC 5 cut(s) 29, 61, 283, 289, 855
Lsp1109I GCAGC 2 cut(s) 1366, 1699
LweI GCATC 8 cut(s) 21, 168, 336, 991, 1206, 1329, 1341, 1502
MaeII ACGT 3 cut(s) 143, 453, 588
MalI GATC 7 cut(s) 26, 237, 443, 648, 669, 1133, 1484
MboI GATC 7 cut(s) 24, 235, 441, 646, 667, 1131, 1482
MboII GAAGA 8 cut(s) 400, 401, 433, 1033, 1040, 1059, 1078, 1624
MfeI CAATTG 1 cut(s) 496
MflI RGATCY 3 cut(s) 235, 1131, 1482
MhlI GDGCHC 7 cut(s) 34, 44, 200, 294, 519, 852, 1239
MlsI TGGCCA 2 cut(s) 530, 1409
MluCI AATT 5 cut(s) 150, 496, 900, 1082, 1321
MluNI TGGCCA 2 cut(s) 530, 1409
MlyI GAGTC 2 cut(s) 415, 590
MmeI TCCRAC 2 cut(s) 333, 808
Mox20I TGGCCA 2 cut(s) 530, 1409
Mph1103I ATGCAT 1 cut(s) 1450
MscI TGGCCA 2 cut(s) 530, 1409
MseI TTAA 3 cut(s) 639, 729, 944
MslI CAYNNNNRTG 4 cut(s) 576, 743, 812, 1337
Msp20I TGGCCA 2 cut(s) 530, 1409
MspA1I CMGCKG 1 cut(s) 110
MspR9I CCNGG 8 cut(s) 229, 254, 310, 486, 897, 1219, 1268, 1577
MunI CAATTG 1 cut(s) 496
MvaI CCWGG 3 cut(s) 254, 897, 1577
MvnI CGCG 7 cut(s) 104, 110, 263, 404, 482, 491, 1541
NciI CCSGG 5 cut(s) 229, 310, 486, 1219, 1268
NcoI CCATGG 2 cut(s) 1120, 1306
NdeII GATC 7 cut(s) 24, 235, 441, 646, 667, 1131, 1482
NlaIII CATG 6 cut(s) 448, 565, 769, 1117, 1124, 1310
NmeAIII GCCGAG 3 cut(s) 538, 1071, 1696
NmuCI GTSAC 5 cut(s) 55, 120, 361, 803, 1007
NsiI ATGCAT 1 cut(s) 1450
OliI CACNNNNGTG 3 cut(s) 576, 743, 812
PagI TCATGA 1 cut(s) 444
PaqCI CACCTGC 2 cut(s) 833, 1427
PciSI GCTCTTC 1 cut(s) 416
PcsI WCGNNNNNNNCGW 2 cut(s) 140, 1341
PfeI GAWTC 4 cut(s) 83, 382, 959, 1263
Pfl23II CGTACG 1 cut(s) 258
PfoI TCCNGGA 2 cut(s) 895, 1266
PkrI GCNGC 5 cut(s) 265, 307, 737, 1356, 1714
PleI GAGTC 2 cut(s) 414, 590
PpsI GAGTC 2 cut(s) 414, 590
Psp124BI GAGCTC 3 cut(s) 519, 852, 1239
Psp6I CCWGG 3 cut(s) 252, 895, 1575
PspEI GGTNACC 1 cut(s) 1274
PspGI CCWGG 3 cut(s) 252, 895, 1575
PspLI CGTACG 1 cut(s) 258
PspPI GGNCC 6 cut(s) 303, 724, 836, 992, 1117, 1221
PsuI RGATCY 3 cut(s) 235, 1131, 1482
RsaI GTAC 6 cut(s) 260, 494, 569, 1100, 1250, 1428
RsaNI GTAC 6 cut(s) 259, 493, 568, 1099, 1249, 1427
RseI CAYNNNNRTG 4 cut(s) 576, 743, 812, 1337
SacI GAGCTC 3 cut(s) 519, 852, 1239
SacII CCGCGG 1 cut(s) 111
SapI GCTCTTC 1 cut(s) 416
SaqAI TTAA 3 cut(s) 639, 729, 944
SatI GCNGC 5 cut(s) 264, 306, 736, 1355, 1713
Sau3AI GATC 7 cut(s) 24, 235, 441, 646, 667, 1131, 1482
Sau96I GGNCC 6 cut(s) 303, 724, 836, 992, 1117, 1221
SchI GAGTC 2 cut(s) 415, 590
ScrFI CCNGG 8 cut(s) 229, 254, 310, 486, 897, 1219, 1268, 1577
SduI GDGCHC 7 cut(s) 34, 44, 200, 294, 519, 852, 1239
SfaNI GCATC 8 cut(s) 21, 168, 336, 991, 1206, 1329, 1341, 1502
Sfr303I CCGCGG 1 cut(s) 111
SgrAI CRCCGGYG 1 cut(s) 124
SgrBI CCGCGG 1 cut(s) 111
SinI GGWCC 4 cut(s) 724, 836, 1117, 1221
SmiMI CAYNNNNRTG 4 cut(s) 576, 743, 812, 1337
SmlI CTYRAG 1 cut(s) 1552
SmoI CTYRAG 1 cut(s) 1552
Sse9I AATT 5 cut(s) 150, 496, 900, 1082, 1321
SstI GAGCTC 3 cut(s) 519, 852, 1239
StyD4I CCNGG 8 cut(s) 227, 252, 308, 484, 895, 1217, 1266, 1575
StyI CCWWGG 4 cut(s) 1120, 1306, 1404, 1454
TaaI ACNGT 2 cut(s) 379, 1079
TaiI ACGT 3 cut(s) 146, 456, 591
TaqI TCGA 2 cut(s) 86, 1335
TasI AATT 5 cut(s) 150, 496, 900, 1082, 1321
TauI GCSGC 3 cut(s) 266, 308, 738
TfiI GAWTC 4 cut(s) 83, 382, 959, 1263
Tru1I TTAA 3 cut(s) 639, 729, 944
Tru9I TTAA 3 cut(s) 639, 729, 944
TscAI CASTG 6 cut(s) 39, 581, 670, 750, 1003, 1525
TseFI GTSAC 5 cut(s) 55, 120, 361, 803, 1007
TseI GCWGC 2 cut(s) 1354, 1712
Tsp45I GTSAC 5 cut(s) 55, 120, 361, 803, 1007
TspDTI ATGAA 6 cut(s) 383, 413, 754, 852, 1574, 1634
TspGWI ACGGA 2 cut(s) 74, 842
TspRI CASTG 6 cut(s) 39, 581, 670, 750, 1003, 1525
VpaK11BI GGWCC 4 cut(s) 724, 836, 1117, 1221
XapI RAATTY 2 cut(s) 150, 1082
XcmI CCANNNNNNNNNTGG 2 cut(s) 625, 1208
Zsp2I ATGCAT 1 cut(s) 1450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.