Rorug01G0066000

Probable zinc-ribbon domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001942
Physical Location & Seq
Forward (+)
10710258 .. 10717714
7457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0066000.1

Sequence Viewer

Length: 1560 bp
ATGAAGCTGAGTCCGATGAAATGCAAGCGCGTTGGAATTTCTTCCGTCGCGAAATTACGCAACGTTGAGGTCGAATCGGAGAGGAGCGTGGAGGAGGTAGTCGGATCCGGCGGTGATTTGACTCCTCAGAAGCGGAAGTTGAGGTCCAATTCGTCGTTGCCGATGAAGTGTAAATCGGCTCGCCGGAACCTCAGTGCTTGCACCAGCACTCCTGCAAATGGAGTTGAGAAGTATTTCAGTCAGAAAAAGCCTCAAGCCTTTTGCAAACCACCGATTGTTGCTAAGCCGAATTGGAATCCCAGAGATAAGGAGCAGATGAGGATAGTGAAGGAGGCATTGCACTTGTCAACGGTCCCCTCTACTGTTGCGTGCCGCGATGTGGAGCAGAAGAGGGTTCTGGAGTTCTGCAAGTCATGCATAGAGCAAGAGAAGGCCGGGAGTTTATATGTATGCGGGTGTCCTGGGACCGGAAAGTCCTTGTCCATGGAGAAAGTGAAACAGGTTTTGGTGAATTGGGCAGGAGAGGGTGGATTTCAGGAACGGGATGTGTTGGAATTGAACTGTACATCGCTAACAAAAACTTCTGATATTTTCGCAAAGATACTTGGTAAATACCAACCTCAAAAGAAAGCTAATGGCACTACCTCCCTGCAACTTCTTCAGAAATTATATTCTCAAAAGCCTCAGTCAGATGGGACTAAGATGATGTTGATAATAGCTGATGAGTTGGATTACTTAATCACTAAAGACCGGGCAGTGCTCCATGACCTTTTCATGCTCACAACATACCCATTCTCAAGATGTATACTTTTAGGAGTAGCAAATGCAATCGACTTAGCTGATCGTTTTGTTGCAAAACTTCAGTCATTAAATTGTAAGCCTATGGTTATAACTTTTCGGGCATACTCTAAGGATCAGATTCTCAAGATTCTCCAAGAGAGGCTTATGGCACTACCTTACACTGTTTTTCAACCACATGCATTGGAACTTTGTGCCAGAAAAGTTGCTGCTGCATCTGGAGATATGAGGAAAGCTCTCTGTATTTCCAGGAGTGCAATTGAGATGCTGGAAGCAGAGTTGAGAGAATCTACTGACAAGTTAAATTCATCAACAGCAGAGAATCCATTCTCTGAGCAACAGACAGCTCCAGCTCTTGCTCTGGTGAAAAAACAGGAGATTGATATTGTGAGAATTGATCACATGGCCATTGCTTTGTCAAAGACATTCAAGTCACCAGTAGTGGATACCATACAATGTCTTCCTCAACATCAACAGATTATAATGTGCTCTGCTGCAAAGCATTTCCGTGGTGTGAAGAAAGATACAACTATAGGAGAGTTAAACAAATCTTACATAGACATCTGCAAAGCAACACTAATTCCCCCGGTTGGGAGTTTTGAATTTTCAAGTATGTGCAAAGTGCTAAATGACCAGGGCCTCCTCAAACTCAGTGGCCAATCCAGAGATATAAAATCCAAGAGAGTGACATTGAATGTAGATGAAGGGGACATCACATTTGCATTGCAGGGAATCCGGTTTTTTCGGACTTGTCTTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zinc_ribbon_12

Protein Analysis

519

Amino Acids

57.77

Weight (kDa)

9.28

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_22 PF13401 144 - 261 7.5e-12 AAA domain
Cdc6-ORC-like_ATPase_lid PF22606 302 - 359 1.9e-11 Cdc6/ORC-like, ATPase lid domain
WHD_Cdc6 PF09079 415 - 507 4.1e-20 CDC6, C terminal winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 890, 1280
AasI GACNNNNNNGTC 2 cut(s) 472, 1228
AccI GTMKAC 1 cut(s) 805
AccII CGCG 3 cut(s) 30, 50, 375
AciI CCGC 4 cut(s) 111, 133, 373, 453
AclI AACGTT 1 cut(s) 63
AclWI GGATC 3 cut(s) 99, 112, 921
AcoI YGGCCR 2 cut(s) 1203, 1453
AcsI RAATTY 3 cut(s) 36, 1102, 1400
AcuI CTGAAG 2 cut(s) 644, 845
AfaI GTAC 1 cut(s) 565
AfiI CCNNNNNNNGG 5 cut(s) 218, 379, 467, 1388, 1389
AgsI TTSAA 6 cut(s) 559, 971, 1228, 1400, 1407, 1492
AjnI CCWGG 3 cut(s) 460, 1046, 1431
AjuI GAANNNNNNNTTGG 2 cut(s) 488, 520
AluBI AGCT 7 cut(s) 7, 632, 719, 839, 1034, 1145, 1151
AluI AGCT 7 cut(s) 7, 632, 719, 839, 1034, 1145, 1151
Alw21I GWGCWC 2 cut(s) 762, 1289
AlwI GGATC 3 cut(s) 99, 112, 921
AoxI GGCC 4 cut(s) 432, 1203, 1435, 1453
ApeKI GCWGC 3 cut(s) 1007, 1010, 1292
ApoI RAATTY 3 cut(s) 36, 1102, 1400
ArsI GACNNNNNNTTYG 4 cut(s) 848, 880, 1499, 1531
Asp700I GAANNNNTTC 3 cut(s) 40, 233, 1124
AspLEI GCGC 1 cut(s) 30
AspS9I GGNCC 4 cut(s) 144, 352, 465, 1435
AsuC2I CCSGG 3 cut(s) 436, 752, 1385
AsuHPI GGTGA 4 cut(s) 125, 520, 1174, 1224
AvaII GGWCC 3 cut(s) 144, 352, 465
BalI TGGCCA 2 cut(s) 1205, 1455
BamHI GGATCC 1 cut(s) 104
BbsI GAAGAC 1 cut(s) 1250
Bbv12I GWGCWC 2 cut(s) 762, 1289
BbvI GCAGC 3 cut(s) 994, 997, 1279
BccI CCATC 1 cut(s) 686
BciT130I CCWGG 3 cut(s) 462, 1048, 1433
BciVI GTATCC 1 cut(s) 1237
BclI TGATCA 1 cut(s) 1195
BcnI CCSGG 3 cut(s) 436, 752, 1385
BfmI CTRYAG 1 cut(s) 1329
BfuI GTATCC 1 cut(s) 1237
BisI GCNGC 4 cut(s) 373, 1008, 1011, 1293
BlpI GCTNAGC 1 cut(s) 282
BlsI GCNGC 4 cut(s) 374, 1009, 1012, 1294
Bme1390I CCNGG 6 cut(s) 436, 462, 752, 1048, 1385, 1433
Bme18I GGWCC 3 cut(s) 144, 352, 465
BmgT120I GGNCC 4 cut(s) 144, 352, 465, 1435
BmiI GGNNCC 4 cut(s) 106, 188, 354, 466
BmrFI CCNGG 6 cut(s) 436, 462, 752, 1048, 1385, 1433
BmsI GCATC 2 cut(s) 1022, 1053
BpiI GAAGAC 1 cut(s) 1250
BplI GAGNNNNNCTC 2 cut(s) 1018, 1050
BpmI CTGGAG 3 cut(s) 419, 1038, 1131
Bpu1102I GCTNAGC 1 cut(s) 282
BpuEI CTTGAG 3 cut(s) 237, 781, 908
BpuMI CCSGG 3 cut(s) 436, 752, 1385
BsaJI CCNNGG 5 cut(s) 461, 483, 1306, 1383, 1432
BsaWI WCCGGW 2 cut(s) 467, 1533
BsaXI ACNNNNNCTCC 2 cut(s) 193, 223
Bsc4I CCNNNNNNNGG 5 cut(s) 218, 379, 467, 1388, 1389
Bse1I ACTGG 1 cut(s) 1235
Bse3DI GCAATG 3 cut(s) 335, 1206, 1520
BseBI CCWGG 3 cut(s) 462, 1048, 1433
BseDI CCNNGG 5 cut(s) 461, 483, 1306, 1383, 1432
BseGI GGATG 1 cut(s) 550
BseLI CCNNNNNNNGG 5 cut(s) 218, 379, 467, 1388, 1389
BseMI GCAATG 3 cut(s) 335, 1206, 1520
BseMII CTCAG 5 cut(s) 140, 205, 698, 1122, 1462
BseNI ACTGG 1 cut(s) 1235
BseRI GAGGAG 4 cut(s) 97, 107, 114, 1430
BseXI GCAGC 3 cut(s) 994, 997, 1279
Bsh1236I CGCG 3 cut(s) 30, 50, 375
BshFI GGCC 4 cut(s) 434, 1205, 1437, 1455
BsiHKAI GWGCWC 2 cut(s) 762, 1289
BsiSI CCGG 7 cut(s) 108, 184, 435, 468, 751, 1385, 1534
BslFI GGGAC 4 cut(s) 338, 478, 709, 1520
BslI CCNNNNNNNGG 5 cut(s) 218, 379, 467, 1388, 1389
BsmFI GGGAC 4 cut(s) 338, 478, 709, 1520
BsnI GGCC 4 cut(s) 434, 1205, 1437, 1455
Bsp1286I GDGCHC 2 cut(s) 762, 1289
Bsp1407I TGTACA 1 cut(s) 563
Bsp143I GATC 4 cut(s) 104, 841, 913, 1195
Bsp1720I GCTNAGC 1 cut(s) 282
Bsp19I CCATGG 1 cut(s) 483
Bsp68I TCGCGA 1 cut(s) 50
BspACI CCGC 4 cut(s) 111, 133, 373, 453
BspANI GGCC 4 cut(s) 434, 1205, 1437, 1455
BspCNI CTCAG 5 cut(s) 139, 204, 697, 1123, 1461
BspFNI CGCG 3 cut(s) 30, 50, 375
BspLI GGNNCC 4 cut(s) 106, 188, 354, 466
BspPI GGATC 3 cut(s) 99, 112, 921
BsrDI GCAATG 3 cut(s) 335, 1206, 1520
BsrGI TGTACA 1 cut(s) 563
BsrI ACTGG 1 cut(s) 1235
BssECI CCNNGG 5 cut(s) 461, 483, 1306, 1383, 1432
BssMI GATC 4 cut(s) 104, 841, 913, 1195
BssNAI GTATAC 1 cut(s) 806
BssT1I CCWWGG 1 cut(s) 483
Bst1107I GTATAC 1 cut(s) 806
Bst2UI CCWGG 3 cut(s) 462, 1048, 1433
Bst4CI ACNGT 4 cut(s) 352, 364, 563, 964
Bst6I CTCTTC 1 cut(s) 383
BstAPI GCANNNNNTGC 1 cut(s) 414
BstAUI TGTACA 1 cut(s) 563
BstC8I GCNNGC 4 cut(s) 26, 181, 199, 370
BstDSI CCRYGG 2 cut(s) 483, 1306
BstF5I GGATG 1 cut(s) 550
BstFNI CGCG 3 cut(s) 30, 50, 375
BstHHI GCGC 1 cut(s) 30
BstKTI GATC 4 cut(s) 107, 844, 916, 1198
BstMBI GATC 4 cut(s) 104, 841, 913, 1195
BstMWI GCNNNNNNNGC 1 cut(s) 414
BstNI CCWGG 3 cut(s) 462, 1048, 1433
BstNSI RCATGY 1 cut(s) 980
BstSCI CCNGG 6 cut(s) 434, 460, 750, 1046, 1383, 1431
BstSFI CTRYAG 1 cut(s) 1329
BstUI CGCG 3 cut(s) 30, 50, 375
BstV1I GCAGC 3 cut(s) 994, 997, 1279
BstV2I GAAGAC 1 cut(s) 1250
BstX2I RGATCY 1 cut(s) 104
BstYI RGATCY 1 cut(s) 104
BstZ17I GTATAC 1 cut(s) 806
BsuI GTATCC 1 cut(s) 1237
BsuRI GGCC 4 cut(s) 434, 1205, 1437, 1455
BtgI CCRYGG 2 cut(s) 483, 1306
BtgZI GCGATG 2 cut(s) 390, 552
BtsCI GGATG 1 cut(s) 550
BtsI GCAGTG 1 cut(s) 762
BtsIMutI CAGTG 4 cut(s) 199, 762, 960, 1456
BtuMI TCGCGA 1 cut(s) 50
Cac8I GCNNGC 4 cut(s) 26, 181, 199, 370
CfoI GCGC 1 cut(s) 30
Cfr13I GGNCC 4 cut(s) 144, 352, 465, 1435
Csp6I GTAC 1 cut(s) 564
CspCI CAANNNNNGTGG 4 cut(s) 963, 998, 1432, 1467
CviAII CATG 6 cut(s) 414, 484, 764, 775, 977, 1201
CviQI GTAC 1 cut(s) 564
DpnI GATC 4 cut(s) 106, 843, 915, 1197
DpnII GATC 4 cut(s) 104, 841, 913, 1195
DrdI GACNNNNNNGTC 2 cut(s) 472, 1228
DseDI GACNNNNNNGTC 2 cut(s) 472, 1228
EaeI YGGCCR 2 cut(s) 1203, 1453
Eam1104I CTCTTC 1 cut(s) 383
EarI CTCTTC 1 cut(s) 383
Eco130I CCWWGG 1 cut(s) 483
Eco47I GGWCC 3 cut(s) 144, 352, 465
Eco57I CTGAAG 2 cut(s) 644, 845
EcoO109I RGGNCCY 1 cut(s) 1435
EcoRII CCWGG 3 cut(s) 460, 1046, 1431
EcoT14I CCWWGG 1 cut(s) 483
EcoT22I ATGCAT 2 cut(s) 419, 982
ErhI CCWWGG 1 cut(s) 483
FaeI CATG 6 cut(s) 417, 487, 767, 778, 980, 1204
FalI AAGNNNNNCTT 2 cut(s) 927, 959
FaqI GGGAC 4 cut(s) 338, 478, 709, 1520
FatI CATG 6 cut(s) 413, 483, 763, 774, 976, 1200
FauI CCCGC 1 cut(s) 446
FbaI TGATCA 1 cut(s) 1195
FblI GTMKAC 1 cut(s) 805
Fnu4HI GCNGC 4 cut(s) 373, 1008, 1011, 1293
FokI GGATG 1 cut(s) 557
Fsp4HI GCNGC 4 cut(s) 373, 1008, 1011, 1293
GlaI GCGC 1 cut(s) 29
GluI GCNGC 4 cut(s) 373, 1008, 1011, 1293
GsuI CTGGAG 3 cut(s) 419, 1038, 1131
HaeIII GGCC 4 cut(s) 434, 1205, 1437, 1455
HapII CCGG 7 cut(s) 108, 184, 435, 468, 751, 1385, 1534
HhaI GCGC 1 cut(s) 30
Hin1II CATG 6 cut(s) 417, 487, 767, 778, 980, 1204
Hin6I GCGC 1 cut(s) 28
HinP1I GCGC 1 cut(s) 28
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HinfI GANTC 9 cut(s) 10, 74, 121, 295, 919, 928, 1085, 1120, 1530
HpaII CCGG 7 cut(s) 108, 184, 435, 468, 751, 1385, 1534
HphI GGTGA 4 cut(s) 125, 520, 1174, 1224
Hpy166II GTNNAC 2 cut(s) 348, 806
Hpy188III TCNNGA 8 cut(s) 49, 398, 536, 798, 925, 1017, 1461, 1553
Hpy8I GTNNAC 2 cut(s) 348, 806
Hpy99I CGWCG 2 cut(s) 50, 157
HpyAV CCTTC 3 cut(s) 322, 424, 1496
HpyCH4III ACNGT 4 cut(s) 352, 364, 563, 964
HpyCH4IV ACGT 1 cut(s) 63
HpyF10VI GCNNNNNNNGC 1 cut(s) 414
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 6 cut(s) 417, 487, 767, 778, 980, 1204
HspAI GCGC 1 cut(s) 28
Ksp22I TGATCA 1 cut(s) 1195
Kzo9I GATC 4 cut(s) 104, 841, 913, 1195
LmnI GCTCC 5 cut(s) 84, 310, 382, 765, 1150
Lsp1109I GCAGC 3 cut(s) 994, 997, 1279
LweI GCATC 2 cut(s) 1022, 1053
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 2 cut(s) 1230, 1483
MalI GATC 4 cut(s) 106, 843, 915, 1197
MboI GATC 4 cut(s) 104, 841, 913, 1195
MboII GAAGA 5 cut(s) 33, 400, 650, 1250, 1327
MfeI CAATTG 1 cut(s) 1056
MflI RGATCY 1 cut(s) 104
MhlI GDGCHC 2 cut(s) 762, 1289
MlsI TGGCCA 2 cut(s) 1205, 1455
MluNI TGGCCA 2 cut(s) 1205, 1455
MlyI GAGTC 2 cut(s) 19, 115
MmeI TCCRAC 4 cut(s) 13, 82, 531, 708
Mox20I TGGCCA 2 cut(s) 1205, 1455
Mph1103I ATGCAT 2 cut(s) 419, 982
MroXI GAANNNNTTC 3 cut(s) 40, 233, 1124
MscI TGGCCA 2 cut(s) 1205, 1455
MseI TTAA 4 cut(s) 737, 869, 1100, 1340
MslI CAYNNNNRTG 1 cut(s) 1305
Msp20I TGGCCA 2 cut(s) 1205, 1455
MspI CCGG 7 cut(s) 108, 184, 435, 468, 751, 1385, 1534
MspR9I CCNGG 6 cut(s) 436, 462, 752, 1048, 1385, 1433
MunI CAATTG 1 cut(s) 1056
MvaI CCWGG 3 cut(s) 462, 1048, 1433
MvnI CGCG 3 cut(s) 30, 50, 375
MwoI GCNNNNNNNGC 1 cut(s) 414
NciI CCSGG 3 cut(s) 436, 752, 1385
NcoI CCATGG 1 cut(s) 483
NdeII GATC 4 cut(s) 104, 841, 913, 1195
NlaIII CATG 6 cut(s) 417, 487, 767, 778, 980, 1204
NlaIV GGNNCC 4 cut(s) 106, 188, 354, 466
NmuCI GTSAC 2 cut(s) 1230, 1483
NruI TCGCGA 1 cut(s) 50
NsiI ATGCAT 2 cut(s) 419, 982
NspI RCATGY 1 cut(s) 980
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 158
PdmI GAANNNNTTC 3 cut(s) 40, 233, 1124
PfeI GAWTC 7 cut(s) 74, 295, 919, 928, 1085, 1120, 1530
PfoI TCCNGGA 1 cut(s) 1046
PkrI GCNGC 4 cut(s) 374, 1009, 1012, 1294
PleI GAGTC 2 cut(s) 18, 115
PpsI GAGTC 2 cut(s) 18, 115
PsiI TTATAA 2 cut(s) 890, 1280
Psp1406I AACGTT 1 cut(s) 63
Psp6I CCWGG 3 cut(s) 460, 1046, 1431
PspGI CCWGG 3 cut(s) 460, 1046, 1431
PspN4I GGNNCC 4 cut(s) 106, 188, 354, 466
PspPI GGNCC 4 cut(s) 144, 352, 465, 1435
PsuI RGATCY 1 cut(s) 104
RruI TCGCGA 1 cut(s) 50
RsaI GTAC 1 cut(s) 565
RsaNI GTAC 1 cut(s) 564
RseI CAYNNNNRTG 1 cut(s) 1305
SaqAI TTAA 4 cut(s) 737, 869, 1100, 1340
SatI GCNGC 4 cut(s) 373, 1008, 1011, 1293
Sau3AI GATC 4 cut(s) 104, 841, 913, 1195
Sau96I GGNCC 4 cut(s) 144, 352, 465, 1435
SchI GAGTC 2 cut(s) 19, 115
ScrFI CCNGG 6 cut(s) 436, 462, 752, 1048, 1385, 1433
SduI GDGCHC 2 cut(s) 762, 1289
SfaNI GCATC 2 cut(s) 1022, 1053
SfcI CTRYAG 1 cut(s) 1329
SinI GGWCC 3 cut(s) 144, 352, 465
SmiMI CAYNNNNRTG 1 cut(s) 1305
SmlI CTYRAG 4 cut(s) 252, 796, 923, 1553
SmoI CTYRAG 4 cut(s) 252, 796, 923, 1553
SsiI CCGC 4 cut(s) 111, 133, 373, 453
StyD4I CCNGG 6 cut(s) 434, 460, 750, 1046, 1383, 1431
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 4 cut(s) 352, 364, 563, 964
TaiI ACGT 1 cut(s) 66
TaqI TCGA 2 cut(s) 72, 831
TatI WGTACW 1 cut(s) 563
TauI GCSGC 1 cut(s) 375
TfiI GAWTC 7 cut(s) 74, 295, 919, 928, 1085, 1120, 1530
Tru1I TTAA 4 cut(s) 737, 869, 1100, 1340
Tru9I TTAA 4 cut(s) 737, 869, 1100, 1340
TscAI CASTG 4 cut(s) 199, 762, 967, 1456
TseFI GTSAC 2 cut(s) 1230, 1483
TseI GCWGC 3 cut(s) 1007, 1010, 1292
Tsp45I GTSAC 2 cut(s) 1230, 1483
TspDTI ATGAA 6 cut(s) 17, 32, 179, 763, 1095, 1515
TspGWI ACGGA 2 cut(s) 34, 1295
TspRI CASTG 4 cut(s) 199, 762, 967, 1456
VpaK11BI GGWCC 3 cut(s) 144, 352, 465
XapI RAATTY 3 cut(s) 36, 1102, 1400
XceI RCATGY 1 cut(s) 980
XmiI GTMKAC 1 cut(s) 805
XmnI GAANNNNTTC 3 cut(s) 40, 233, 1124
Zsp2I ATGCAT 2 cut(s) 419, 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.