Rorug04G0062200

Alcohol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001942
Physical Location & Seq
Forward (+)
9729036 .. 9737893
8858 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0062200.1

Sequence Viewer

Length: 2307 bp
ATGAGGGCAGGTTCGTTGTGGGCCGACGAGCCCAATTCTCCTTCTACCTCCGTGCCCCGCGTTCCCCAGCCTAATCCCTCCAAGCACAGTGTTAGCAATGTGTTTAGCTTGTTAGCACGGAGAGAGGTCTCTCCTCAAACAAAACATTCGGCAAAGAAGTTATGGGGGAAAGCTTCCAAGAGTCAATCTGATAACATTGGCCCAAGGTGTGAAGCAGCTAGAGATGCTAGAAATGGCCTTGTGTCATGGGTGGAGGCAGAGTCGCTCCAGCATTTATCTGCAAAATATTGCCCGCTTGTGCCTCCTCCACGGTCAACTATTGCAGCAGCCTTCAGCCCTGATGGAAAGACACTTGCTTCCACTCATGGAGATCATACTGTAAAGATCATTGATTGCCGAACTGGAAGATGCTTAAAGGTGTTGAGTGGTCACCGCAGGACACCTTGGGTGGTCAGGTTTCATCCTTTGCATCCAGATATACTGGCTAGTGGAAGTCTGGATCATGAAGTGAGGTTGTGGGATTCAAACACGGCAGAGTGTATAGGATCGCGTGATTTCTTACGCCCCATTGCTTCCATAGCTTTCCATGCCCAAGGCGATCTTCTTGCTGTTGCATCAGGTCACAAGTTATACATATGGCACTACAATAGAAGAGGAGAGTCATCCTCACCAACAATAGTACTGAAGACACGACGTTCGCTTCGGGCTGTGCATTTTCATCCTCATGCTGCTCCTTTCCTTTTAACTGCTGAGGTCAATGACCTTGACTCGTCAGATTCCTCGATGACACTCGCAACTTCTCCGGGTTACTTGCGCTATCCCCCACCTACTGTGTATTTGGCAGATGCTCATTCCAGTGATCGTACTAGTTTGGCAGATGGACTACCTCTTATGTCTTTACCATTCCTGATATGGCCTTCATTTGCTAGAGATAGTGGGAGAATATCTATGCAGCGTAGTGATGTGGATATTGGTTCAAGTAGTGCACAACAGAGAGTTGATCCTTCTGCTTCTGTGCGGCTCCTTACATATTCAACTCCATCAGGGCAGTATGAACTTCTGTTGTCCCATATTGAACCAAATAGCTCTTCTCCAGCGCCAGAAGACACGGGAAATAGTCCGTTTTTGAGTGAAATGGAAACTGAAGTTTCTCAATCTGCTATGGGTACTTTAGAGCCTATGGAAGTGCAACCAGAAAGCAGAAGTAATCATATTTTCCCTTTTGGTGACTCAACATATTGGGAACTTCCTTTCTTGCAAGGGTGGCTAATTGGTCAGACCCAAGCTAGCCAACGGAATATGCGGCTGGTAAGTGATTCTGCCCATGACAATCCATCTACAAATGGTGAGATGGACAGCCCAGCTCCTATCACTTCCTCAGTAATACCGACCGGTGTAAACCAACCTAGGGTTACTGGAAGATCTAGTTCCCGGCACCGTACTTCACGTTCTCATATGTTGTCAGCAATTGGGTCTAATGAGGGTGCTGGATTCAATAATATCGCTCATGGTGAAAGTGAGCCTCAACCTGTTGTCAGCAGAATCCAATCTGAGCTTGCTAACTCCCTTGCTGCAGCAGCGGCGGCAGAGTTACCTTGTACTGTGAAGCTCAGAGTTTGGCCACATGATGTGAAAAATCCGTGTGCTCCCCTTGATGCTGAAAGATGTTACTTAACTATTCCGCATGCCGTACTTTGTAGTGAAATGGGAGCCCATTTTTCACCTTGTGGAAGGTTTTTAGCAGCCTGTGTTGCATGTATGTTGCCTCATTTGGAAGGTGATCCTGGATTACAGGGCCAGGCCAACCATGATATCACAGGGGCAGCAACCTCCCCAACTCGTCACCCAATTTCAGCTCACCATGTCGTGTATGAGCTCCGGATATATTCCCTCGAGGAGGCAACGTTTGGCATGGTTCTTGCATCCCGGGCAATACGAGCTGCCCATTGCTTGACATCTATTCAGTTCTCTCCTACGTCAGAACACATATTACTTGCGTATGGCCGGCGTCACAGTTCACTTCTTAAGAGTGTTGTCATTGACGGAGAGACAACAGTGCCTATTTACACCATTCTGGAGGTATATAGAGTTTCTGATATGGAACTTGTGAGAATTCTCCCTAGTGCAGAAGATGAGGTCAACGTAGCTTGTTTTCATCCTTCTGTCGGAGGTGGCCTTGTTTATGGAACTAAGGAAGGGAAGTTAAGGATCCTCCAATATGATAGTTCTCATGCCGTGGGTCATACAACTTCCAGTTTTCTTGATGAAAACATGCTAGAGGTCCCAACTTATGCTTTAGAATGCTAG

Protein Analysis

768

Amino Acids

83.56

Weight (kDa)

6.42

Isoelectric Point (pI)

55.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 108 - 243 4.9e-20 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 108 - 213 3.4e-12 WDR3 first beta-propeller domain
WDR55 PF24796 108 - 210 5.2e-08 WDR55
Beta-prop_WDR5 PF25175 110 - 253 7.5e-20 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 110 - 217 5e-12 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 110 - 209 8.6e-10 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 111 - 216 4.2e-07 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 114 - 241 1.1e-10 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 149 - 242 6.2e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1892
AccB1I GGYRCC 1 cut(s) 1434
AccII CGCG 2 cut(s) 60, 550
AccIII TCCGGA 1 cut(s) 1878
AciI CCGC 8 cut(s) 58, 293, 433, 1018, 1303, 1580, 1583, 1682
AclI AACGTT 1 cut(s) 1904
AclWI GGATC 6 cut(s) 507, 553, 995, 1775, 2203, 2216
AcoI YGGCCR 2 cut(s) 1619, 2002
AcsI RAATTY 1 cut(s) 2112
AcuI CTGAAG 3 cut(s) 316, 704, 1164
AcyI GRCGYC 1 cut(s) 2008
AdeI CACNNNGTG 1 cut(s) 1727
AfaI GTAC 6 cut(s) 681, 865, 1168, 1441, 1600, 1692
AfiI CCNNNNNNNGG 3 cut(s) 1408, 1897, 2165
AflII CTTAAG 1 cut(s) 2024
AgeI ACCGGT 1 cut(s) 1391
AgsI TTSAA 5 cut(s) 525, 978, 1035, 1076, 1495
AhlI ACTAGT 1 cut(s) 866
AjnI CCWGG 2 cut(s) 1783, 1797
AjuI GAANNNNNNNTTGG 4 cut(s) 585, 617, 1072, 1104
Alw21I GWGCWC 3 cut(s) 988, 1648, 1878
Alw26I GTCTC 2 cut(s) 133, 2042
Alw44I GTGCAC 1 cut(s) 984
AlwI GGATC 6 cut(s) 507, 553, 995, 1775, 2203, 2216
Ama87I CYCGRG 2 cut(s) 1892, 1926
Aor13HI TCCGGA 1 cut(s) 1878
AoxI GGCC 9 cut(s) 21, 199, 235, 914, 1619, 1795, 1800, 2002, 2173
ApaLI GTGCAC 1 cut(s) 984
ApoI RAATTY 1 cut(s) 2112
ArsI GACNNNNNNTTYG 4 cut(s) 679, 684, 711, 716
AsiGI ACCGGT 1 cut(s) 1391
AspA2I CCTAGG 1 cut(s) 1406
AspLEI GCGC 2 cut(s) 816, 1099
AspS9I GGNCC 4 cut(s) 21, 200, 1795, 2281
AsuC2I CCSGG 4 cut(s) 804, 1432, 1927, 1928
AsuHPI GGTGA 9 cut(s) 422, 660, 1238, 1358, 1523, 1713, 1790, 1835, 1850
AsuNHI GCTAGC 1 cut(s) 1286
AvaI CYCGRG 2 cut(s) 1892, 1926
AvaII GGWCC 1 cut(s) 2281
AvrII CCTAGG 1 cut(s) 1406
BaeGI GKGCMC 2 cut(s) 57, 988
BalI TGGCCA 1 cut(s) 1621
BamHI GGATCC 1 cut(s) 2208
BanI GGYRCC 1 cut(s) 1434
BanII GRGCYC 3 cut(s) 33, 1714, 1878
BbsI GAAGAC 2 cut(s) 692, 1110
Bbv12I GWGCWC 3 cut(s) 988, 1648, 1878
BbvCI CCTCAGC 1 cut(s) 750
BccI CCATC 5 cut(s) 335, 872, 1048, 1342, 1345
BceAI ACGGC 3 cut(s) 546, 1673, 2219
BcgI CGANNNNNNTGC 2 cut(s) 587, 621
BciT130I CCWGG 2 cut(s) 1785, 1799
BcnI CCSGG 4 cut(s) 804, 1432, 1927, 1928
BcoDI GTCTC 2 cut(s) 133, 2042
BcuI ACTAGT 1 cut(s) 866
BfmI CTRYAG 1 cut(s) 1572
BfoI RGCGCY 1 cut(s) 1100
BfrI CTTAAG 1 cut(s) 2024
BglII AGATCT 1 cut(s) 1421
BlnI CCTAGG 1 cut(s) 1406
BmcAI AGTACT 1 cut(s) 681
Bme1390I CCNGG 6 cut(s) 804, 1432, 1785, 1799, 1927, 1928
Bme18I GGWCC 1 cut(s) 2281
BmeT110I CYCGRG 2 cut(s) 1892, 1926
BmgT120I GGNCC 4 cut(s) 21, 200, 1795, 2281
BmiI GGNNCC 5 cut(s) 1022, 1436, 1711, 2210, 2283
BmrFI CCNGG 6 cut(s) 804, 1432, 1785, 1799, 1927, 1928
BmsI GCATC 7 cut(s) 214, 398, 478, 623, 835, 1645, 1931
BmtI GCTAGC 1 cut(s) 1290
BpiI GAAGAC 2 cut(s) 692, 1110
BplI GAGNNNNNCTC 2 cut(s) 650, 682
BpmI CTGGAG 3 cut(s) 251, 1077, 2096
Bpu10I CCTNAGC 1 cut(s) 750
BpuMI CCSGG 4 cut(s) 804, 1432, 1927, 1928
BsaHI GRCGYC 1 cut(s) 2008
BsaI GGTCTC 1 cut(s) 133
BsaJI CCNNGG 7 cut(s) 203, 308, 443, 592, 1406, 1926, 2235
BsaWI WCCGGW 2 cut(s) 1391, 1878
BsaXI ACNNNNNCTCC 4 cut(s) 245, 275, 1889, 1919
Bsc4I CCNNNNNNNGG 3 cut(s) 1408, 1897, 2165
Bse118I RCCGGY 2 cut(s) 1391, 2004
Bse1I ACTGG 5 cut(s) 406, 486, 855, 1420, 2253
Bse3DI GCAATG 3 cut(s) 103, 567, 1945
BseAI TCCGGA 1 cut(s) 1878
BseBI CCWGG 2 cut(s) 1785, 1799
BseDI CCNNGG 7 cut(s) 203, 308, 443, 592, 1406, 1926, 2235
BseGI GGATG 6 cut(s) 460, 469, 662, 718, 1922, 2155
BseLI CCNNNNNNNGG 3 cut(s) 1408, 1897, 2165
BseMI GCAATG 3 cut(s) 103, 567, 1945
BseMII CTCAG 4 cut(s) 741, 1392, 1542, 1624
BseNI ACTGG 5 cut(s) 406, 486, 855, 1420, 2253
BseRI GAGGAG 4 cut(s) 123, 294, 669, 1910
BseSI GKGCMC 2 cut(s) 57, 988
BseYI CCCAGC 2 cut(s) 66, 1360
BsgI GTGCAG 1 cut(s) 2145
Bsh1236I CGCG 2 cut(s) 60, 550
Bsh1285I CGRYCG 1 cut(s) 1392
BshFI GGCC 9 cut(s) 23, 201, 237, 916, 1621, 1797, 1802, 2004, 2175
BshNI GGYRCC 1 cut(s) 1434
BshTI ACCGGT 1 cut(s) 1391
BsiEI CGRYCG 1 cut(s) 1392
BsiHKAI GWGCWC 3 cut(s) 988, 1648, 1878
BsiHKCI CYCGRG 2 cut(s) 1892, 1926
BsiSI CCGG 6 cut(s) 803, 1392, 1432, 1879, 1927, 2005
BslFI GGGAC 2 cut(s) 1051, 2267
BslI CCNNNNNNNGG 3 cut(s) 1408, 1897, 2165
BsmAI GTCTC 2 cut(s) 133, 2042
BsmFI GGGAC 2 cut(s) 1051, 2267
BsmI GAATGC 1 cut(s) 2306
BsnI GGCC 9 cut(s) 23, 201, 237, 916, 1621, 1797, 1802, 2004, 2175
Bso31I GGTCTC 1 cut(s) 133
BsoBI CYCGRG 2 cut(s) 1892, 1926
Bsp1286I GDGCHC 6 cut(s) 33, 57, 988, 1648, 1714, 1878
Bsp13I TCCGGA 1 cut(s) 1878
BspACI CCGC 8 cut(s) 58, 293, 433, 1018, 1303, 1580, 1583, 1682
BspANI GGCC 9 cut(s) 23, 201, 237, 916, 1621, 1797, 1802, 2004, 2175
BspCNI CTCAG 4 cut(s) 742, 1391, 1543, 1623
BspEI TCCGGA 1 cut(s) 1878
BspFNI CGCG 2 cut(s) 60, 550
BspHI TCATGA 1 cut(s) 502
BspLI GGNNCC 5 cut(s) 1022, 1436, 1711, 2210, 2283
BspMAI CTGCAG 1 cut(s) 1576
BspOI GCTAGC 1 cut(s) 1290
BspPI GGATC 6 cut(s) 507, 553, 995, 1775, 2203, 2216
BspQI GCTCTTC 1 cut(s) 1093
BspT107I GGYRCC 1 cut(s) 1434
BspTI CTTAAG 1 cut(s) 2024
BspTNI GGTCTC 1 cut(s) 133
BsrDI GCAATG 3 cut(s) 103, 567, 1945
BsrFI RCCGGY 2 cut(s) 1391, 2004
BsrI ACTGG 5 cut(s) 406, 486, 855, 1420, 2253
BssAI RCCGGY 2 cut(s) 1391, 2004
BssECI CCNNGG 7 cut(s) 203, 308, 443, 592, 1406, 1926, 2235
BssNI GRCGYC 1 cut(s) 2008
BssT1I CCWWGG 4 cut(s) 203, 443, 592, 1406
Bst2UI CCWGG 2 cut(s) 1785, 1799
Bst4CI ACNGT 8 cut(s) 89, 312, 379, 832, 1439, 1603, 2015, 2056
Bst6I CTCTTC 2 cut(s) 646, 1093
BstACI GRCGYC 1 cut(s) 2008
BstAFI CTTAAG 1 cut(s) 2024
BstC8I GCNNGC 5 cut(s) 293, 1288, 1557, 1686, 2006
BstDEI CTNAG 5 cut(s) 750, 1378, 1551, 1610, 2190
BstDSI CCRYGG 2 cut(s) 308, 2235
BstEII GGTNACC 1 cut(s) 428
BstENI CCTNNNNNAGG 1 cut(s) 1895
BstF5I GGATG 6 cut(s) 460, 469, 662, 718, 1922, 2155
BstFNI CGCG 2 cut(s) 60, 550
BstH2I RGCGCY 1 cut(s) 1100
BstHHI GCGC 2 cut(s) 816, 1099
BstMAI GTCTC 2 cut(s) 133, 2042
BstMCI CGRYCG 1 cut(s) 1392
BstNI CCWGG 2 cut(s) 1785, 1799
BstNSI RCATGY 3 cut(s) 1688, 1758, 2275
BstPI GGTNACC 1 cut(s) 428
BstSCI CCNGG 6 cut(s) 802, 1430, 1783, 1797, 1925, 1926
BstSFI CTRYAG 1 cut(s) 1572
BstSLI GKGCMC 2 cut(s) 57, 988
BstUI CGCG 2 cut(s) 60, 550
BstV2I GAAGAC 2 cut(s) 692, 1110
BstX2I RGATCY 2 cut(s) 1421, 2208
BstYI RGATCY 2 cut(s) 1421, 2208
BsuRI GGCC 9 cut(s) 23, 201, 237, 916, 1621, 1797, 1802, 2004, 2175
BtgI CCRYGG 2 cut(s) 308, 2235
BtsCI GGATG 6 cut(s) 460, 469, 662, 718, 1922, 2155
BtsIMutI CAGTG 3 cut(s) 94, 862, 2061
Cac8I GCNNGC 5 cut(s) 293, 1288, 1557, 1686, 2006
CciI TCATGA 1 cut(s) 502
CfoI GCGC 2 cut(s) 816, 1099
Cfr10I RCCGGY 2 cut(s) 1391, 2004
Cfr13I GGNCC 4 cut(s) 21, 200, 1795, 2281
Cfr9I CCCGGG 1 cut(s) 1926
CseI GACGC 1 cut(s) 1997
Csp6I GTAC 6 cut(s) 680, 864, 1167, 1440, 1599, 1691
CspAI ACCGGT 1 cut(s) 1391
CviQI GTAC 6 cut(s) 680, 864, 1167, 1440, 1599, 1691
DdeI CTNAG 5 cut(s) 750, 1378, 1551, 1610, 2190
DraIII CACNNNGTG 1 cut(s) 1727
EaeI YGGCCR 2 cut(s) 1619, 2002
Eam1104I CTCTTC 2 cut(s) 646, 1093
EarI CTCTTC 2 cut(s) 646, 1093
Ecl136II GAGCTC 1 cut(s) 1876
Eco130I CCWWGG 4 cut(s) 203, 443, 592, 1406
Eco24I GRGCYC 3 cut(s) 33, 1714, 1878
Eco31I GGTCTC 1 cut(s) 133
Eco32I GATATC 1 cut(s) 1813
Eco47I GGWCC 1 cut(s) 2281
Eco53kI GAGCTC 1 cut(s) 1876
Eco57I CTGAAG 3 cut(s) 316, 704, 1164
Eco88I CYCGRG 2 cut(s) 1892, 1926
Eco91I GGTNACC 1 cut(s) 428
EcoICRI GAGCTC 1 cut(s) 1876
EcoNI CCTNNNNNAGG 1 cut(s) 1895
EcoO109I RGGNCCY 1 cut(s) 2281
EcoO65I GGTNACC 1 cut(s) 428
EcoRI GAATTC 1 cut(s) 2112
EcoRII CCWGG 2 cut(s) 1783, 1797
EcoRV GATATC 1 cut(s) 1813
EcoT14I CCWWGG 4 cut(s) 203, 443, 592, 1406
EcoT38I GRGCYC 3 cut(s) 33, 1714, 1878
ErhI CCWWGG 4 cut(s) 203, 443, 592, 1406
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 2 cut(s) 1051, 2267
FauI CCCGC 2 cut(s) 65, 300
FauNDI CATATG 2 cut(s) 635, 1455
FokI GGATG 6 cut(s) 447, 456, 649, 705, 1909, 2142
FriOI GRGCYC 3 cut(s) 33, 1714, 1878
GlaI GCGC 2 cut(s) 815, 1098
GsaI CCCAGC 2 cut(s) 70, 1364
GsuI CTGGAG 3 cut(s) 251, 1077, 2096
HaeII RGCGCY 1 cut(s) 1100
HaeIII GGCC 9 cut(s) 23, 201, 237, 916, 1621, 1797, 1802, 2004, 2175
HapII CCGG 6 cut(s) 803, 1392, 1432, 1879, 1927, 2005
HgaI GACGC 1 cut(s) 1997
HhaI GCGC 2 cut(s) 816, 1099
Hin1I GRCGYC 1 cut(s) 2008
Hin6I GCGC 2 cut(s) 814, 1097
HinP1I GCGC 2 cut(s) 814, 1097
HincII GTYRAC 2 cut(s) 315, 2140
HindII GTYRAC 2 cut(s) 315, 2140
HindIII AAGCTT 1 cut(s) 171
HpaII CCGG 6 cut(s) 803, 1392, 1432, 1879, 1927, 2005
HphI GGTGA 9 cut(s) 422, 660, 1238, 1358, 1523, 1713, 1790, 1835, 1850
Hpy166II GTNNAC 5 cut(s) 315, 986, 1399, 2018, 2140
Hpy188I TCNGA 8 cut(s) 190, 775, 1278, 1552, 1613, 1981, 2095, 2168
Hpy188III TCNNGA 7 cut(s) 473, 497, 503, 907, 1879, 2075, 2261
Hpy8I GTNNAC 5 cut(s) 315, 986, 1399, 2018, 2140
Hpy99I CGWCG 2 cut(s) 29, 696
HpyAV CCTTC 8 cut(s) 51, 340, 927, 1014, 1725, 1769, 2169, 2189
HpyCH4III ACNGT 8 cut(s) 89, 312, 379, 832, 1439, 1603, 2015, 2056
HpyCH4IV ACGT 5 cut(s) 694, 1447, 1904, 1976, 2142
HpyF3I CTNAG 5 cut(s) 750, 1378, 1551, 1610, 2190
HpySE526I ACGT 5 cut(s) 694, 1447, 1904, 1976, 2142
Hsp92I GRCGYC 1 cut(s) 2008
HspAI GCGC 2 cut(s) 814, 1097
Kpn2I TCCGGA 1 cut(s) 1878
KroI GCCGGC 1 cut(s) 2004
KroNI GCCGGC 1 cut(s) 2006
LguI GCTCTTC 1 cut(s) 1093
LmnI GCTCC 7 cut(s) 270, 736, 1026, 1369, 1651, 1709, 1881
LweI GCATC 7 cut(s) 214, 398, 478, 623, 835, 1645, 1931
MaeII ACGT 5 cut(s) 694, 1447, 1904, 1976, 2142
MaeIII GTNAC 9 cut(s) 428, 620, 806, 1226, 1411, 1590, 1667, 1841, 2009
MboII GAAGA 8 cut(s) 417, 593, 663, 697, 1080, 1115, 1431, 2141
MfeI CAATTG 1 cut(s) 1467
MflI RGATCY 2 cut(s) 1421, 2208
MhlI GDGCHC 6 cut(s) 33, 57, 988, 1648, 1714, 1878
MlsI TGGCCA 1 cut(s) 1621
MluCI AATT 5 cut(s) 34, 1269, 1467, 1848, 2112
MluNI TGGCCA 1 cut(s) 1621
MlyI GAGTC 5 cut(s) 190, 269, 668, 761, 1223
MmeI TCCRAC 1 cut(s) 2146
Mox20I TGGCCA 1 cut(s) 1621
MroI TCCGGA 1 cut(s) 1878
MroNI GCCGGC 1 cut(s) 2004
MscI TGGCCA 1 cut(s) 1621
MseI TTAA 5 cut(s) 413, 743, 1673, 2025, 2204
MslI CAYNNNNRTG 2 cut(s) 723, 855
Msp20I TGGCCA 1 cut(s) 1621
MspA1I CMGCKG 1 cut(s) 1580
MspCI CTTAAG 1 cut(s) 2024
MspI CCGG 6 cut(s) 803, 1392, 1432, 1879, 1927, 2005
MspR9I CCNGG 6 cut(s) 804, 1432, 1785, 1799, 1927, 1928
MunI CAATTG 1 cut(s) 1467
Mva1269I GAATGC 1 cut(s) 2306
MvaI CCWGG 2 cut(s) 1785, 1799
MvnI CGCG 2 cut(s) 60, 550
NaeI GCCGGC 1 cut(s) 2006
NciI CCSGG 4 cut(s) 804, 1432, 1927, 1928
NdeI CATATG 2 cut(s) 635, 1455
NgoMIV GCCGGC 1 cut(s) 2004
NheI GCTAGC 1 cut(s) 1286
NlaIV GGNNCC 5 cut(s) 1022, 1436, 1711, 2210, 2283
NmuCI GTSAC 5 cut(s) 428, 620, 1226, 1841, 2009
NspI RCATGY 3 cut(s) 1688, 1758, 2275
PaeI GCATGC 1 cut(s) 1688
PaeR7I CTCGAG 1 cut(s) 1892
PagI TCATGA 1 cut(s) 502
PciSI GCTCTTC 1 cut(s) 1093
PctI GAATGC 1 cut(s) 2306
PdiI GCCGGC 1 cut(s) 2006
PfeI GAWTC 5 cut(s) 521, 776, 1316, 1491, 1542
PfoI TCCNGGA 1 cut(s) 1783
PinAI ACCGGT 1 cut(s) 1391
PleI GAGTC 5 cut(s) 189, 268, 667, 761, 1223
PpsI GAGTC 5 cut(s) 189, 268, 667, 761, 1223
PpuMI RGGWCCY 1 cut(s) 2281
Psp124BI GAGCTC 1 cut(s) 1878
Psp1406I AACGTT 1 cut(s) 1904
Psp5II RGGWCCY 1 cut(s) 2281
Psp6I CCWGG 2 cut(s) 1783, 1797
PspEI GGTNACC 1 cut(s) 428
PspFI CCCAGC 2 cut(s) 66, 1360
PspGI CCWGG 2 cut(s) 1783, 1797
PspN4I GGNNCC 5 cut(s) 1022, 1436, 1711, 2210, 2283
PspPI GGNCC 4 cut(s) 21, 200, 1795, 2281
PspPPI RGGWCCY 1 cut(s) 2281
PspXI VCTCGAGB 1 cut(s) 1892
PsrI GAACNNNNNNTAC 4 cut(s) 1432, 1464, 1974, 2006
PstI CTGCAG 1 cut(s) 1576
PsuI RGATCY 2 cut(s) 1421, 2208
RsaI GTAC 6 cut(s) 681, 865, 1168, 1441, 1600, 1692
RsaNI GTAC 6 cut(s) 680, 864, 1167, 1440, 1599, 1691
RseI CAYNNNNRTG 2 cut(s) 723, 855
SacI GAGCTC 1 cut(s) 1878
SapI GCTCTTC 1 cut(s) 1093
SaqAI TTAA 5 cut(s) 413, 743, 1673, 2025, 2204
Sau96I GGNCC 4 cut(s) 21, 200, 1795, 2281
ScaI AGTACT 1 cut(s) 681
SchI GAGTC 5 cut(s) 190, 269, 668, 761, 1223
ScrFI CCNGG 6 cut(s) 804, 1432, 1785, 1799, 1927, 1928
SduI GDGCHC 6 cut(s) 33, 57, 988, 1648, 1714, 1878
SfaNI GCATC 7 cut(s) 214, 398, 478, 623, 835, 1645, 1931
SfcI CTRYAG 1 cut(s) 1572
Sfr274I CTCGAG 1 cut(s) 1892
SinI GGWCC 1 cut(s) 2281
SlaI CTCGAG 1 cut(s) 1892
SmaI CCCGGG 1 cut(s) 1928
SmiMI CAYNNNNRTG 2 cut(s) 723, 855
SmlI CTYRAG 2 cut(s) 1892, 2024
SmoI CTYRAG 2 cut(s) 1892, 2024
SpeI ACTAGT 1 cut(s) 866
SphI GCATGC 1 cut(s) 1688
Sse9I AATT 5 cut(s) 34, 1269, 1467, 1848, 2112
SsiI CCGC 8 cut(s) 58, 293, 433, 1018, 1303, 1580, 1583, 1682
SspI AATATT 1 cut(s) 287
SstI GAGCTC 1 cut(s) 1878
StyD4I CCNGG 6 cut(s) 802, 1430, 1783, 1797, 1925, 1926
StyI CCWWGG 4 cut(s) 203, 443, 592, 1406
TaaI ACNGT 8 cut(s) 89, 312, 379, 832, 1439, 1603, 2015, 2056
TaiI ACGT 5 cut(s) 697, 1450, 1907, 1979, 2145
TaqI TCGA 2 cut(s) 782, 1893
TasI AATT 5 cut(s) 34, 1269, 1467, 1848, 2112
TatI WGTACW 2 cut(s) 679, 1598
TauI GCSGC 4 cut(s) 1021, 1306, 1583, 1586
TfiI GAWTC 5 cut(s) 521, 776, 1316, 1491, 1542
Tru1I TTAA 5 cut(s) 413, 743, 1673, 2025, 2204
Tru9I TTAA 5 cut(s) 413, 743, 1673, 2025, 2204
TscAI CASTG 3 cut(s) 94, 862, 2061
TseFI GTSAC 5 cut(s) 428, 620, 1226, 1841, 2009
Tsp45I GTSAC 5 cut(s) 428, 620, 1226, 1841, 2009
TspDTI ATGAA 7 cut(s) 449, 519, 707, 909, 1068, 2144, 2280
TspGWI ACGGA 6 cut(s) 40, 133, 1110, 1309, 1629, 2058
TspMI CCCGGG 1 cut(s) 1926
TspRI CASTG 3 cut(s) 94, 862, 2061
Vha464I CTTAAG 1 cut(s) 2024
VneI GTGCAC 1 cut(s) 984
VpaK11BI GGWCC 1 cut(s) 2281
XagI CCTNNNNNAGG 1 cut(s) 1895
XapI RAATTY 1 cut(s) 2112
XceI RCATGY 3 cut(s) 1688, 1758, 2275
XcmI CCANNNNNNNNNTGG 1 cut(s) 909
XhoI CTCGAG 1 cut(s) 1892
XmaI CCCGGG 1 cut(s) 1926
XmaJI CCTAGG 1 cut(s) 1406
ZrmI AGTACT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.