Rh1DG009100

Alcohol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001983
Physical Location & Seq
Forward (+)
1505723 .. 1509110
3388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG009100.1

Sequence Viewer

Length: 810 bp
ATGCTCGATGGAACTTCAAGAATGTCGACTGTCAAGGGACAAAAGATGCTGTACCACCTCTTTTCTTGCTCCACATGGTCCGAGTACATGGTCGTTAACGTCAATTTCCTTGTCAAGCTCCAAAAATGGGGTGCTCATCGTACTACTACACCACCTCTGTCCCACGCTAGCTTCCTCTCATGTGGATTCTCTACCGGATATGGGGCTCCTTGGAAGGAAGCTAAGATTGAAAGAGGGTCAACTGTTGCTGTTATTGGTCTTGGTGCTGTTGGATTAGGGGCAGTTGAGGGGGCTAGAATGCAAGGTGCATCTAGGATAATCGGTGTCGACAAAAATGAAATGAAAAGAGAAAAAGGAATAGCTTTTGGAATGACTGATTTTATAAACCCTGACAATCACCAATCTGAACATGATCAAGATCATGACAAATCAGTTTCTAAGCTCATCAAAGACTGTACAGATGGAATGGGTGTAGATTATTGCTTTGAGTGCACTGGCGTTGCATCCTTCATCAATGAAGCCCTTGGAGCCACAAAAATGGGTAAAGGAACAGCAGTTGTGCTAGGAGGCTCAGGTGTAACAACCGTTCAAATTGATTTTCTCTCCCTGATGAGCGGCAGAACCTTGAAAGGGTCCCTGTTTGGAGGTCTCAAAGCCAAAACCGACCTTCCCATTCTCATCAACAAATGCATGAATATGGAAATGCAATTGGATGAACTCTTGACTCATGAAGTTCCTCTCGTAGATATTAACAAAGCACTTGAATTCTTAAAGCAGCCAGATTGTGTGAAGGTTCTGATCAAGATTTGA

Protein Analysis

269

Amino Acids

29.16

Weight (kDa)

7.6

Isoelectric Point (pI)

25.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_zinc_N PF00107 89 - 220 5.6e-22 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 383
AccBSI CCGCTC 1 cut(s) 615
AccI GTMKAC 2 cut(s) 26, 327
AciI CCGC 1 cut(s) 615
AcsI RAATTY 1 cut(s) 764
AfaI GTAC 4 cut(s) 53, 86, 142, 457
AfiI CCNNNNNNNGG 3 cut(s) 127, 201, 630
AgsI TTSAA 5 cut(s) 18, 230, 590, 628, 764
AluBI AGCT 5 cut(s) 118, 171, 221, 362, 442
AluI AGCT 5 cut(s) 118, 171, 221, 362, 442
Alw21I GWGCWC 2 cut(s) 136, 494
Alw26I GTCTC 1 cut(s) 653
Alw44I GTGCAC 1 cut(s) 490
ApaLI GTGCAC 1 cut(s) 490
ApeKI GCWGC 1 cut(s) 775
ApoI RAATTY 1 cut(s) 764
AspS9I GGNCC 2 cut(s) 78, 633
AsuHPI GGTGA 1 cut(s) 389
AsuNHI GCTAGC 1 cut(s) 167
AvaII GGWCC 2 cut(s) 78, 633
BaeGI GKGCMC 1 cut(s) 494
BanII GRGCYC 1 cut(s) 208
Bbv12I GWGCWC 2 cut(s) 136, 494
BbvI GCAGC 1 cut(s) 787
BccI CCATC 2 cut(s) 2, 455
BclI TGATCA 2 cut(s) 412, 798
BcoDI GTCTC 1 cut(s) 653
BfaI CTAG 4 cut(s) 168, 294, 312, 563
BisI GCNGC 2 cut(s) 616, 776
BlsI GCNGC 2 cut(s) 617, 777
Bme18I GGWCC 2 cut(s) 78, 633
BmgT120I GGNCC 2 cut(s) 78, 633
BmiI GGNNCC 4 cut(s) 207, 529, 634, 635
BmsI GCATC 3 cut(s) 36, 317, 512
BmtI GCTAGC 1 cut(s) 171
Bpu10I CCTNAGC 1 cut(s) 571
BsaBI GATNNNNATC 1 cut(s) 417
BsaI GGTCTC 1 cut(s) 653
BsaJI CCNNGG 2 cut(s) 209, 523
BsaWI WCCGGW 1 cut(s) 194
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 201, 630
Bse1I ACTGG 1 cut(s) 499
Bse8I GATNNNNATC 1 cut(s) 417
BseDI CCNNGG 2 cut(s) 209, 523
BseGI GGATG 2 cut(s) 503, 718
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 3 cut(s) 127, 201, 630
BseMII CTCAG 1 cut(s) 585
BseNI ACTGG 1 cut(s) 499
BseSI GKGCMC 1 cut(s) 494
BseXI GCAGC 1 cut(s) 787
BsiHKAI GWGCWC 2 cut(s) 136, 494
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 3 cut(s) 51, 145, 619
BslI CCNNNNNNNGG 3 cut(s) 127, 201, 630
BsmAI GTCTC 1 cut(s) 653
BsmFI GGGAC 3 cut(s) 51, 145, 619
BsmI GAATGC 1 cut(s) 303
Bso31I GGTCTC 1 cut(s) 653
Bsp1286I GDGCHC 3 cut(s) 136, 208, 494
Bsp1407I TGTACA 1 cut(s) 455
Bsp143I GATC 3 cut(s) 412, 418, 798
BspACI CCGC 1 cut(s) 615
BspCNI CTCAG 1 cut(s) 584
BspHI TCATGA 2 cut(s) 421, 727
BspLI GGNNCC 4 cut(s) 207, 529, 634, 635
BspOI GCTAGC 1 cut(s) 171
BspTNI GGTCTC 1 cut(s) 653
BsrBI CCGCTC 1 cut(s) 615
BsrGI TGTACA 1 cut(s) 455
BsrI ACTGG 1 cut(s) 499
BssECI CCNNGG 2 cut(s) 209, 523
BssMI GATC 3 cut(s) 412, 418, 798
BssT1I CCWWGG 2 cut(s) 209, 523
Bst4CI ACNGT 4 cut(s) 31, 244, 455, 586
BstAUI TGTACA 1 cut(s) 455
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 3 cut(s) 222, 438, 571
BstF5I GGATG 2 cut(s) 503, 718
BstKTI GATC 3 cut(s) 415, 421, 801
BstMAI GTCTC 1 cut(s) 653
BstMBI GATC 3 cut(s) 412, 418, 798
BstMWI GCNNNNNNNGC 2 cut(s) 489, 527
BstSLI GKGCMC 1 cut(s) 494
BstV1I GCAGC 1 cut(s) 787
BstXI CCANNNNNNTGG 1 cut(s) 538
BtsCI GGATG 2 cut(s) 503, 718
BtsIMutI CAGTG 1 cut(s) 492
Cac8I GCNNGC 1 cut(s) 169
CciI TCATGA 2 cut(s) 421, 727
Cfr13I GGNCC 2 cut(s) 78, 633
Csp6I GTAC 4 cut(s) 52, 85, 141, 456
CviAII CATG 7 cut(s) 75, 88, 180, 410, 422, 691, 728
CviQI GTAC 4 cut(s) 52, 85, 141, 456
DdeI CTNAG 3 cut(s) 222, 438, 571
DpnI GATC 3 cut(s) 414, 420, 800
DpnII GATC 3 cut(s) 412, 418, 798
Eco130I CCWWGG 2 cut(s) 209, 523
Eco24I GRGCYC 1 cut(s) 208
Eco31I GGTCTC 1 cut(s) 653
Eco47I GGWCC 2 cut(s) 78, 633
EcoO109I RGGNCCY 1 cut(s) 633
EcoRI GAATTC 1 cut(s) 764
EcoT14I CCWWGG 2 cut(s) 209, 523
EcoT22I ATGCAT 1 cut(s) 692
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 2 cut(s) 209, 523
FaeI CATG 7 cut(s) 78, 91, 183, 413, 425, 694, 731
FaqI GGGAC 3 cut(s) 51, 145, 619
FatI CATG 7 cut(s) 74, 87, 179, 409, 421, 690, 727
FbaI TGATCA 2 cut(s) 412, 798
FblI GTMKAC 2 cut(s) 26, 327
Fnu4HI GCNGC 2 cut(s) 616, 776
FokI GGATG 2 cut(s) 490, 725
FriOI GRGCYC 1 cut(s) 208
Fsp4HI GCNGC 2 cut(s) 616, 776
FspBI CTAG 4 cut(s) 168, 294, 312, 563
GluI GCNGC 2 cut(s) 616, 776
HapII CCGG 1 cut(s) 195
Hin1II CATG 7 cut(s) 78, 91, 183, 413, 425, 694, 731
HincII GTYRAC 4 cut(s) 27, 97, 240, 328
HindII GTYRAC 4 cut(s) 27, 97, 240, 328
HinfI GANTC 2 cut(s) 186, 724
HpaI GTTAAC 1 cut(s) 97
HpaII CCGG 1 cut(s) 195
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 5 cut(s) 27, 97, 240, 328, 492
Hpy188I TCNGA 3 cut(s) 82, 406, 798
Hpy188III TCNNGA 6 cut(s) 18, 416, 422, 721, 728, 802
Hpy8I GTNNAC 5 cut(s) 27, 97, 240, 328, 492
HpyAV CCTTC 4 cut(s) 208, 517, 677, 784
HpyCH4III ACNGT 4 cut(s) 31, 244, 455, 586
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 6 cut(s) 301, 308, 492, 503, 690, 706
HpyF10VI GCNNNNNNNGC 2 cut(s) 489, 527
HpyF3I CTNAG 3 cut(s) 222, 438, 571
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 7 cut(s) 78, 91, 183, 413, 425, 694, 731
KflI GGGWCCC 1 cut(s) 633
Ksp22I TGATCA 2 cut(s) 412, 798
KspAI GTTAAC 1 cut(s) 97
Kzo9I GATC 3 cut(s) 412, 418, 798
LmnI GCTCC 4 cut(s) 74, 123, 211, 527
LpnPI CCDG 7 cut(s) 208, 402, 480, 558, 620, 650, 792
Lsp1109I GCAGC 1 cut(s) 787
LweI GCATC 3 cut(s) 36, 317, 512
MaeI CTAG 4 cut(s) 168, 294, 312, 563
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 1 cut(s) 577
MalI GATC 3 cut(s) 414, 420, 800
MbiI CCGCTC 1 cut(s) 615
MboI GATC 3 cut(s) 412, 418, 798
MfeI CAATTG 1 cut(s) 707
MhlI GDGCHC 3 cut(s) 136, 208, 494
MluCI AATT 4 cut(s) 103, 591, 707, 764
MlyI GAGTC 1 cut(s) 718
MmeI TCCRAC 1 cut(s) 250
MnlI CCTC 8 cut(s) 68, 165, 185, 227, 280, 560, 638, 747
Mph1103I ATGCAT 1 cut(s) 692
MseI TTAA 3 cut(s) 96, 750, 770
MslI CAYNNNNRTG 2 cut(s) 536, 695
MspI CCGG 1 cut(s) 195
MunI CAATTG 1 cut(s) 707
Mva1269I GAATGC 1 cut(s) 303
MwoI GCNNNNNNNGC 2 cut(s) 489, 527
NdeII GATC 3 cut(s) 412, 418, 798
NheI GCTAGC 1 cut(s) 167
NlaIII CATG 7 cut(s) 78, 91, 183, 413, 425, 694, 731
NlaIV GGNNCC 4 cut(s) 207, 529, 634, 635
NsiI ATGCAT 1 cut(s) 692
PagI TCATGA 2 cut(s) 421, 727
PctI GAATGC 1 cut(s) 303
PfeI GAWTC 1 cut(s) 186
PkrI GCNGC 2 cut(s) 617, 777
PleI GAGTC 1 cut(s) 718
PpsI GAGTC 1 cut(s) 718
PpuMI RGGWCCY 1 cut(s) 633
PsiI TTATAA 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 633
PspN4I GGNNCC 4 cut(s) 207, 529, 634, 635
PspPI GGNCC 2 cut(s) 78, 633
PspPPI RGGWCCY 1 cut(s) 633
PsrI GAACNNNNNNTAC 2 cut(s) 570, 602
RsaI GTAC 4 cut(s) 53, 86, 142, 457
RsaNI GTAC 4 cut(s) 52, 85, 141, 456
RseI CAYNNNNRTG 2 cut(s) 536, 695
SalI GTCGAC 2 cut(s) 25, 326
SaqAI TTAA 3 cut(s) 96, 750, 770
SatI GCNGC 2 cut(s) 616, 776
Sau3AI GATC 3 cut(s) 412, 418, 798
Sau96I GGNCC 2 cut(s) 78, 633
SchI GAGTC 1 cut(s) 718
SduI GDGCHC 3 cut(s) 136, 208, 494
SfaNI GCATC 3 cut(s) 36, 317, 512
SinI GGWCC 2 cut(s) 78, 633
SmiMI CAYNNNNRTG 2 cut(s) 536, 695
Sse9I AATT 4 cut(s) 103, 591, 707, 764
SsiI CCGC 1 cut(s) 615
SspMI CTAG 4 cut(s) 168, 294, 312, 563
StyI CCWWGG 2 cut(s) 209, 523
TaaI ACNGT 4 cut(s) 31, 244, 455, 586
TaiI ACGT 1 cut(s) 102
TaqI TCGA 3 cut(s) 6, 26, 327
TasI AATT 4 cut(s) 103, 591, 707, 764
TatI WGTACW 2 cut(s) 84, 455
TauI GCSGC 1 cut(s) 618
TfiI GAWTC 1 cut(s) 186
Tru1I TTAA 3 cut(s) 96, 750, 770
Tru9I TTAA 3 cut(s) 96, 750, 770
TscAI CASTG 1 cut(s) 499
TseI GCWGC 1 cut(s) 775
TspDTI ATGAA 7 cut(s) 351, 356, 499, 531, 707, 729, 744
TspRI CASTG 1 cut(s) 499
VneI GTGCAC 1 cut(s) 490
VpaK11BI GGWCC 2 cut(s) 78, 633
XapI RAATTY 1 cut(s) 764
XmiI GTMKAC 2 cut(s) 26, 327
XspI CTAG 4 cut(s) 168, 294, 312, 563
Zsp2I ATGCAT 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.