Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0003686

Physical Location & Seq

Genomic Coordinates Reverse (-)

31352740 .. 31363281

Gene Structure 10542 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0178700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3102 bp

ATGGATCTTCCTGGCCTTGCTGTTATACTCCAAGCGGCTCTCAGCACCAATCCCGATGAACGGAAAGCCGCCGAGCACAGCCTCAATCAGATTCAGTATACACCGCAGCATTTGGTGAGACTGTTGCAAATTATTGTGGATGGTAACTGCGACATGGGTGTGCGTCAGGTTGCCAGCATCCATTTCAAGAACTTCATTGCGAAGAACTGGTCACCTCATGAGCCGGATGAGCAGAATAAGATATCACAAGCTGATAAGGAGGTGGTTAGGGAGCACGTTCTTGTGTTTGTTACACAGGTTCCCCCTTTGTTGAGGGTGCAACTGGGTGAGTGCCTAAAGACAATTATTCATGCCGATTACCCTGAGCAGTGGCCACGGCTTTTAGACTGGGTGAAGCACAACTTACAAGACCAACAAGTTTATGGAGCTTTGTTTGTTTTGAGGATTCTTTCTAGAAAATACGAGTTCAAATCTGATGAGGAGAGAACACCTGTTTATCTTATTGTTGAGGAGACATTCCCACATCTGCTCAATATATTCAACCGGCTTGTCCAGATTCCCAACCCATCACTGGAAGTAGCAGATTTGATCAAGCTTATCTGTAAAATTTTCTGGTCATCCATTTATTTGGAGATTCCAAAGCAGCTATTTGATCCCAATGTGTTCAATGCTTGGATGATGCTTTTCCTAAATATATTGGAGAGACCAGTTCCAGTGGAAGGCCAACCTGCAGATCCTGATCTTAGAAAGGCATGGGGGTGGTGGAAGGTGAAGAAATGGACTATCCACATTTTAAACCGGTTGTACACACGGTTCGGAGATTTGAAGCTCCAAAACCCAGAAAACAGGGCATTTGCTCAAATGTTTCAGAAGAGTTACGCTGGGAAGATTTTGGAGTGCCACTTGAATTTGCTGAATGTGATTCGAACGGGTGGATACTTACCTGACAGAGTTACCAATCTTGTTCTTCAATATTTAAGCAATAGTATCTCAAAGATTAGCATGTATACTCTGCTGCAACCACGACTTAATGTTCTTCTTTTTGAGATAGTTTTTCCCCTGATGTGTTTCAATGACAATGACCAAAAGCTATGGGATGAAGACCCACATGAATATGTGAGGAAGGGCTACGATATCATTGAAGACTTATACAGTCCAAGAACAGCTTCCATGGATTTTGTCAGTGAGTTGGTCAGGAAACGTGGAAAGGAAAATCTTCATACGTTCCTTCAATTCATTGTGGAAATTTTCAAGAGGTACGATGAAGCCCCGATGGAATACAAACCCTATCGACAGAAAGATGGCGCGCTTCTTGCTATTGGAGCACTTTGTGACAAATTGAAACAAACGGAACCTTACAAGTCTGAATTAGAGCGTATGTTAGTGCAACATGTATTCCCCGAGTTTAGTAGTCCTGTTGGGCATCTTAGAGCTAAGGCAGCATGGGTTGCAGGACAGTATGCCCATATTAACTTCTCAGAGCCGAACAACTTCCTTAAAGCATTGCATAGTGTGGTCGCAGGGATGCGAGATCCAGAACTTCCTGTTCGAGTTGATTCAGTGTTTGCTTTGCGTTCCTTTGTTGAAGCTTGCAGAGATTTGAATGAAATTCGCCCAATTCTTCCTCAGCTACTTGATGAGTTTTTCAAGCTTATGAATGAGGTTGAGAATGAAGATCTGGTTTTTACCCTGGAGACTATAGTGGACAAGTTTGGGGAGGAAATGGCACCATATGCTCTTGGTTTGTGTCAAAATCTGGCAGCCGCATTCTGGAGGTGTATGAATACAGCTGAAGCTGATGATGACGCCGATGATCCTGGTGCTTTGGCTGCTGTTGGTTGTTTGCGTGCTATTAGCACCATTTTAGAATCAGTGAGCAGGCTCCCACATCTCTTTGTCCAAGTTGAACCAACTTTGCTTCCTATAATGCGTAGAATGTTGACAACCGATGGGCAAGAGGTCTTTGAAGAGGTGTTGGAAATTGTATCGTATATGACATTTTTCTCTCCAACAATATCTTTGGATATGTGGAGTCTTTGGCCATTGATGATGGAAGCTTTAGCCGATTGGGCTATAGATTTCTTTTCAAATATTCTGGTTCCTTTGGATAACTACATATCGAGGGGGACTGCACACTTTTTATCTTGTAAAGAACCAGACTACCAGCAAAGCCTTTGGAATATGATTTCATCTATCCTGGCAGATAGGAATATGGAAGATGGTGATATTGAGCCTGCTCCTAAGCTCATTCAAGTACTTTTTCAGAATTGCAGAGGGCAGGTTGATCAGTGGGTTGAGCCCTATATTAGAATTACATTTGAACGGTTACGTCGAGCTGAAAAATCATACTTGAAATGTCTGCTTGTTCAAGTGATTGCAGATGCTCTTTACTACAATGCAGCTTTGACACTCAACATATTGCAAAAGCTGGGTATAGCGACAGATCTATTTAATCTTTGGTTCCAGATGTTGCAAGAGGTTAAGAAAAGTGGCGTACGTGTGCATTTTAAAAGGGAACATGACAAAAAGGTTTGCTGCTTGGGCTTGACATCATTACTGACACTCCCAGCTGGACAATTACCTGCGGAGGCTTTGGGCCGTGTTTTCAGGGCCACCCTTGATCTCTTAGTTGCATACAAGGATCAAGTTGCAGCTGCTGCAAAGGAGGAAGAAGCTGAAGATGATGATGATATGGATGGTTTCCAAACTGATGATGACGATGATGGCGGGGATGGATCAGACAAGGAAATGGGAGTTGATGCCGAGGATGGTGATGAAGCTGATAGCCTTAAATTTCAAAAGTTAGCTGCACAGGCAAAGTGTTTCCGCCCAAGTGATGAGTTCGATGACGACTCTGATGAAGATTTCAGTGATGATGAGGAGTTACAGTCACCAATTGACGATGTGGATCCTTTTGTCTTCTTTGTAGATGCAGTCAAATCCGTGCAAGCATCGGATCCGCCTAGGTTTCAGAGTCTTACACAGACGCTAGACTTCCATTATCAGGCACTTGCAAATGGTGTAGCACAGCATGCTGAACAGAGGAGAGCAGAGATTGAAAAAGAAAAGATGGAGAAGGCTTCAGTTGCCACAGCTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001525 GO:0001568 GO:0001944 GO:0003008 GO:0003013 GO:0003073 GO:0003085 GO:0003674 GO:0003824 GO:0004177 GO:0006508 GO:0006807 GO:0006950 GO:0007275 GO:0008015 GO:0008150 GO:0008152 GO:0008217 GO:0008233 GO:0008238 GO:0008239 GO:0009611 GO:0009653 GO:0010594 GO:0010632 GO:0016787 GO:0019538 GO:0030334 GO:0032501 GO:0032502 GO:0032879 GO:0035239 GO:0035295 GO:0040012 GO:0042060 GO:0043170 GO:0043535 GO:0044238 GO:0045776 GO:0048514 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051239 GO:0051270 GO:0060055 GO:0065007 GO:0065008 GO:0070011 GO:0071704 GO:0072358 GO:0072359 GO:0140096 GO:1901564 GO:2000145

KEGG Pathways

Metabolic & Signaling

ko04614 ko04974 map04614 map04974

Pfam Domains

Protein Families

Peptidase_S28

Protein Analysis

1033

Amino Acids

118.45

Weight (kDa)

4.86

Isoelectric Point (pI)

41.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	24 - 96	1.4e-15	Importin-beta N-terminal domain
Cse1	PF08506	153 - 450	3.8e-09	Cse1
HEAT_IPO9_c	PF25018	358 - 549	5.1e-06	Importin-9 central HEAT repeats
TPR_IPO11	PF25758	634 - 894	3.8e-08	Importin-11-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003686)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g29120 FvH4_1g29701 FvH4_2g10340 FvH4_3g26319 FvH4_4g05781 FvH4_4g16191 FvH4_4g17212 FvH4_4g18132 FvH4_6g16754 FvH4_6g23731
rosa_chinensis	RchiOBHm_Chr0c29g0501111 RchiOBHm_Chr2g0126951 RchiOBHm_Chr2g0133761 RchiOBHm_Chr4g0407741 RchiOBHm_Chr6g0278911
rosa_laevigata	RLG00000034039
rosa_multiflora	Rmu_co8166508.1_g000001 Rmu_sc0002986.1_g000053 Rmu_sc0005664.1_g000049
rosa_roxburghii	Rroxscaffold_3G00250140 Rroxscaffold_5G00362530 Rroxscaffold_6G00423300
rosa_rugosa	Rorug04G0115900 Rorug04G0116000 Rorug04G0116000 Rorug04G0178600 Rorug04G0178700 Rorug04G0178800 Rorug04G0178800 Rorug05G0495800
rosa_samantha	Rh1CG126400 Rh4AG111400 Rh4BG376900 Rh6BG350300 Rh6BG405500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	736, 2269, 2590
AccB1I	GGYRCC	1 cut(s)	1726
AccI	GTMKAC	2 cut(s)	98, 1007
AccII	CGCG	1 cut(s)	1307
AciI	CCGC	8 cut(s)	35, 69, 104, 1764, 2585, 2727, 2827, 2960
AcoI	YGGCCR	2 cut(s)	371, 2039
AcsI	RAATTY	5 cut(s)	606, 907, 1245, 1608, 2792
AcuI	CTGAAG	3 cut(s)	1812, 2697, 3066
AcyI	GRCGYC	1 cut(s)	1806
AdeI	CACNNNGTG	1 cut(s)	1331
AfaI	GTAC	4 cut(s)	806, 1259, 2256, 2496
AfiI	CCNNNNNNNGG	5 cut(s)	60, 571, 719, 1770, 3004
AflIII	ACRYGT	2 cut(s)	1390, 2497
AgeI	ACCGGT	1 cut(s)	798
AjnI	CCWGG	4 cut(s)	10, 1689, 1816, 2196
AjuI	GAANNNNNNNTTGG	2 cut(s)	1150, 1182
Alw21I	GWGCWC	3 cut(s)	78, 276, 1327
Alw26I	GTCTC	4 cut(s)	112, 506, 697, 1688
AlwNI	CAGNNNCTG	2 cut(s)	737, 2657
Ama87I	CYCGRG	1 cut(s)	1400
AoxI	GGCC	6 cut(s)	13, 371, 721, 2039, 2596, 2610
ApoI	RAATTY	5 cut(s)	606, 907, 1245, 1608, 2792
ArsI	GACNNNNNNTTYG	2 cut(s)	194, 226
AsiGI	ACCGGT	1 cut(s)	798
Asp700I	GAANNNNTTC	3 cut(s)	1165, 1215, 1490
AspA2I	CCTAGG	1 cut(s)	2963
AspLEI	GCGC	2 cut(s)	1307, 1309
AspS9I	GGNCC	2 cut(s)	2596, 2610
AsuHPI	GGTGA	8 cut(s)	127, 204, 338, 403, 781, 2234, 2783, 2883
AsuII	TTCGAA	1 cut(s)	925
AvaI	CYCGRG	1 cut(s)	1400
AvrII	CCTAGG	1 cut(s)	2963
BalI	TGGCCA	2 cut(s)	373, 2041
BamHI	GGATCC	2 cut(s)	2908, 2956
BanI	GGYRCC	1 cut(s)	1726
BanII	GRGCYC	1 cut(s)	2301
BbsI	GAAGAC	3 cut(s)	1107, 1149, 2911
Bbv12I	GWGCWC	3 cut(s)	78, 276, 1327
BbvCI	CCTCAGC	1 cut(s)	1626
BceAI	ACGGC	2 cut(s)	392, 2583
BciT130I	CCWGG	4 cut(s)	12, 1691, 1818, 2198
BciVI	GTATCC	1 cut(s)	929
BclI	TGATCA	2 cut(s)	588, 2284
BcoDI	GTCTC	4 cut(s)	112, 506, 697, 1688
BfaI	CTAG	3 cut(s)	453, 2964, 2990
BfmI	CTRYAG	3 cut(s)	729, 1698, 2073
BfuAI	ACCTGC	3 cut(s)	736, 2269, 2590
BfuI	GTATCC	1 cut(s)	929
BglI	GCCNNNNNGGC	1 cut(s)	2069
BglII	AGATCT	2 cut(s)	1675, 2443
BlnI	CCTAGG	1 cut(s)	2963
BmcAI	AGTACT	1 cut(s)	2256
Bme1390I	CCNGG	4 cut(s)	12, 1691, 1818, 2198
BmeT110I	CYCGRG	1 cut(s)	1400
BmgT120I	GGNCC	2 cut(s)	2596, 2610
BmiI	GGNNCC	8 cut(s)	300, 1353, 1728, 1883, 2100, 2462, 2910, 2958
BmrFI	CCNGG	4 cut(s)	12, 1691, 1818, 2198
BmrI	ACTGGG	2 cut(s)	332, 397
BmsI	GCATC	8 cut(s)	186, 669, 1432, 1515, 2371, 2749, 2920, 2960
BmuI	ACTGGG	2 cut(s)	332, 397
BpiI	GAAGAC	3 cut(s)	1107, 1149, 2911
BpmI	CTGGAG	2 cut(s)	1712, 1792
Bpu10I	CCTNAGC	4 cut(s)	363, 1434, 1626, 2241
Bpu14I	TTCGAA	1 cut(s)	925
BsaAI	YACGTR	1 cut(s)	2498
BsaBI	GATNNNNATC	2 cut(s)	738, 2907
BsaHI	GRCGYC	1 cut(s)	1806
BsaI	GGTCTC	1 cut(s)	697
BsaJI	CCNNGG	5 cut(s)	374, 1170, 1689, 2763, 2963
BsaWI	WCCGGW	1 cut(s)	798
BsaXI	ACNNNNNCTCC	4 cut(s)	2547, 2577, 2873, 2903
Bsc4I	CCNNNNNNNGG	5 cut(s)	60, 571, 719, 1770, 3004
Bse118I	RCCGGY	2 cut(s)	543, 798
Bse1I	ACTGG	6 cut(s)	212, 327, 392, 576, 707, 713
Bse3DI	GCAATG	2 cut(s)	195, 1502
Bse8I	GATNNNNATC	2 cut(s)	738, 2907
BseBI	CCWGG	4 cut(s)	12, 1691, 1818, 2198
BseDI	CCNNGG	5 cut(s)	374, 1170, 1689, 2763, 2963
BseJI	GATNNNNATC	2 cut(s)	738, 2907
BseLI	CCNNNNNNNGG	5 cut(s)	60, 571, 719, 1770, 3004
BseMI	GCAATG	2 cut(s)	195, 1502
BseMII	CTCAG	4 cut(s)	55, 354, 1491, 1640
BseNI	ACTGG	6 cut(s)	212, 327, 392, 576, 707, 713
BsePI	GCGCGC	1 cut(s)	1305
BseRI	GAGGAG	4 cut(s)	494, 524, 2894, 3058
BseYI	CCCAGC	3 cut(s)	881, 2428, 2566
BsgI	GTGCAG	2 cut(s)	2115, 2793
Bsh1236I	CGCG	1 cut(s)	1307
BshFI	GGCC	6 cut(s)	15, 373, 723, 2041, 2598, 2612
BshNI	GGYRCC	1 cut(s)	1726
BshTI	ACCGGT	1 cut(s)	798
BsiHKAI	GWGCWC	3 cut(s)	78, 276, 1327
BsiHKCI	CYCGRG	1 cut(s)	1400
BsiSI	CCGG	3 cut(s)	224, 544, 799
BsiWI	CGTACG	1 cut(s)	2494
BslFI	GGGAC	1 cut(s)	2140
BslI	CCNNNNNNNGG	5 cut(s)	60, 571, 719, 1770, 3004
BsmAI	GTCTC	4 cut(s)	112, 506, 697, 1688
BsmFI	GGGAC	1 cut(s)	2140
BsmI	GAATGC	1 cut(s)	1766
BsnI	GGCC	6 cut(s)	15, 373, 723, 2041, 2598, 2612
Bso31I	GGTCTC	1 cut(s)	697
BsoBI	CYCGRG	1 cut(s)	1400
Bsp119I	TTCGAA	1 cut(s)	925
Bsp1286I	GDGCHC	4 cut(s)	78, 276, 1327, 2301
Bsp1407I	TGTACA	1 cut(s)	804
Bsp19I	CCATGG	1 cut(s)	1170
BspACI	CCGC	8 cut(s)	35, 69, 104, 1764, 2585, 2727, 2827, 2960
BspANI	GGCC	6 cut(s)	15, 373, 723, 2041, 2598, 2612
BspCNI	CTCAG	4 cut(s)	54, 355, 1490, 1639
BspFNI	CGCG	1 cut(s)	1307
BspHI	TCATGA	2 cut(s)	217, 3098
BspLI	GGNNCC	8 cut(s)	300, 1353, 1728, 1883, 2100, 2462, 2910, 2958
BspMAI	CTGCAG	1 cut(s)	733
BspMI	ACCTGC	3 cut(s)	736, 2269, 2590
BspT104I	TTCGAA	1 cut(s)	925
BspT107I	GGYRCC	1 cut(s)	1726
BspTNI	GGTCTC	1 cut(s)	697
BsrDI	GCAATG	2 cut(s)	195, 1502
BsrFI	RCCGGY	2 cut(s)	543, 798
BsrGI	TGTACA	1 cut(s)	804
BsrI	ACTGG	6 cut(s)	212, 327, 392, 576, 707, 713
BssAI	RCCGGY	2 cut(s)	543, 798
BssECI	CCNNGG	5 cut(s)	374, 1170, 1689, 2763, 2963
BssHII	GCGCGC	1 cut(s)	1305
BssNAI	GTATAC	2 cut(s)	99, 1008
BssNI	GRCGYC	1 cut(s)	1806
BssT1I	CCWWGG	2 cut(s)	1170, 2963
Bst1107I	GTATAC	2 cut(s)	99, 1008
Bst2UI	CCWGG	4 cut(s)	12, 1691, 1818, 2198
Bst4CI	ACNGT	6 cut(s)	123, 813, 1154, 1458, 2325, 2889
Bst6I	CTCTTC	2 cut(s)	866, 1962
BstACI	GRCGYC	1 cut(s)	1806
BstAPI	GCANNNNNTGC	4 cut(s)	1448, 1733, 2657, 3032
BstAUI	TGTACA	1 cut(s)	804
BstBAI	YACGTR	1 cut(s)	2498
BstBI	TTCGAA	1 cut(s)	925
BstC8I	GCNNGC	8 cut(s)	175, 1307, 1591, 1848, 1880, 2235, 2949, 3033
BstDEI	CTNAG	9 cut(s)	41, 363, 743, 1427, 1434, 1477, 1626, 2241, 2626
BstDSI	CCRYGG	2 cut(s)	374, 1170
BstEII	GGTNACC	1 cut(s)	210
BstFNI	CGCG	1 cut(s)	1307
BstHHI	GCGC	2 cut(s)	1307, 1309
BstMAI	GTCTC	4 cut(s)	112, 506, 697, 1688
BstNI	CCWGG	4 cut(s)	12, 1691, 1818, 2198
BstNSI	RCATGY	3 cut(s)	1006, 1394, 3035
BstPI	GGTNACC	1 cut(s)	210
BstSCI	CCNGG	4 cut(s)	10, 1689, 1816, 2196
BstSFI	CTRYAG	3 cut(s)	729, 1698, 2073
BstUI	CGCG	1 cut(s)	1307
BstV2I	GAAGAC	3 cut(s)	1107, 1149, 2911
BstX2I	RGATCY	7 cut(s)	4, 733, 1531, 1675, 2443, 2908, 2956
BstXI	CCANNNNNNTGG	1 cut(s)	628
BstYI	RGATCY	7 cut(s)	4, 733, 1531, 1675, 2443, 2908, 2956
BstZ17I	GTATAC	2 cut(s)	99, 1008
BsuI	GTATCC	1 cut(s)	929
BsuRI	GGCC	6 cut(s)	15, 373, 723, 2041, 2598, 2612
BtgI	CCRYGG	2 cut(s)	374, 1170
BtsI	GCAGTG	1 cut(s)	374
BtsIMutI	CAGTG	8 cut(s)	374, 569, 720, 1189, 1566, 1878, 2294, 2875
BveI	ACCTGC	3 cut(s)	736, 2269, 2590
Cac8I	GCNNGC	8 cut(s)	175, 1307, 1591, 1848, 1880, 2235, 2949, 3033
CaiI	CAGNNNCTG	2 cut(s)	737, 2657
CciI	TCATGA	2 cut(s)	217, 3098
CfoI	GCGC	2 cut(s)	1307, 1309
Cfr10I	RCCGGY	2 cut(s)	543, 798
Cfr13I	GGNCC	2 cut(s)	2596, 2610
CseI	GACGC	3 cut(s)	152, 1814, 2995
Csp6I	GTAC	4 cut(s)	805, 1258, 2255, 2495
CspAI	ACCGGT	1 cut(s)	798
CviQI	GTAC	4 cut(s)	805, 1258, 2255, 2495
DdeI	CTNAG	9 cut(s)	41, 363, 743, 1427, 1434, 1477, 1626, 2241, 2626
DraI	TTTAAA	2 cut(s)	795, 2509
DraIII	CACNNNGTG	1 cut(s)	1331
EaeI	YGGCCR	2 cut(s)	371, 2039
Eam1104I	CTCTTC	2 cut(s)	866, 1962
EarI	CTCTTC	2 cut(s)	866, 1962
EciI	GGCGGA	2 cut(s)	2816, 2949
Eco130I	CCWWGG	2 cut(s)	1170, 2963
Eco24I	GRGCYC	1 cut(s)	2301
Eco31I	GGTCTC	1 cut(s)	697
Eco32I	GATATC	2 cut(s)	243, 1135
Eco57I	CTGAAG	3 cut(s)	1812, 2697, 3066
Eco88I	CYCGRG	1 cut(s)	1400
Eco91I	GGTNACC	1 cut(s)	210
EcoO65I	GGTNACC	1 cut(s)	210
EcoRII	CCWGG	4 cut(s)	10, 1689, 1816, 2196
EcoRV	GATATC	2 cut(s)	243, 1135
EcoT14I	CCWWGG	2 cut(s)	1170, 2963
EcoT38I	GRGCYC	1 cut(s)	2301
ErhI	CCWWGG	2 cut(s)	1170, 2963
FalI	AAGNNNNNCTT	4 cut(s)	386, 418, 1150, 1182
FaqI	GGGAC	1 cut(s)	2140
FauI	CCCGC	1 cut(s)	2720
FauNDI	CATATG	1 cut(s)	1732
FbaI	TGATCA	2 cut(s)	588, 2284
FblI	GTMKAC	2 cut(s)	98, 1007
FriOI	GRGCYC	1 cut(s)	2301
FspBI	CTAG	3 cut(s)	453, 2964, 2990
GlaI	GCGC	2 cut(s)	1306, 1308
GsaI	CCCAGC	3 cut(s)	885, 2432, 2570
GsuI	CTGGAG	2 cut(s)	1712, 1792
HaeIII	GGCC	6 cut(s)	15, 373, 723, 2041, 2598, 2612
HapII	CCGG	3 cut(s)	224, 544, 799
HgaI	GACGC	3 cut(s)	152, 1814, 2995
HhaI	GCGC	2 cut(s)	1307, 1309
Hin1I	GRCGYC	1 cut(s)	1806
Hin6I	GCGC	2 cut(s)	1305, 1307
HinP1I	GCGC	2 cut(s)	1305, 1307
HincII	GTYRAC	1 cut(s)	1941
HindII	GTYRAC	1 cut(s)	1941
HindIII	AAGCTT	4 cut(s)	593, 1587, 1649, 2055
HpaII	CCGG	3 cut(s)	224, 544, 799
HphI	GGTGA	8 cut(s)	127, 204, 338, 403, 781, 2234, 2783, 2883
Hpy166II	GTNNAC	5 cut(s)	99, 807, 1008, 1705, 1941
Hpy8I	GTNNAC	5 cut(s)	99, 807, 1008, 1705, 1941
Hpy99I	CGWCG	1 cut(s)	2334
HpyAV	CCTTC	5 cut(s)	713, 760, 1117, 1238, 3070
HpyCH4III	ACNGT	6 cut(s)	123, 813, 1154, 1458, 2325, 2889
HpyCH4IV	ACGT	5 cut(s)	276, 1201, 1223, 2329, 2497
HpyF3I	CTNAG	9 cut(s)	41, 363, 743, 1427, 1434, 1477, 1626, 2241, 2626
HpySE526I	ACGT	5 cut(s)	276, 1201, 1223, 2329, 2497
Hsp92I	GRCGYC	1 cut(s)	1806
HspAI	GCGC	2 cut(s)	1305, 1307
Ksp22I	TGATCA	2 cut(s)	588, 2284
LmnI	GCTCC	6 cut(s)	271, 425, 834, 1322, 1887, 2242
LweI	GCATC	8 cut(s)	186, 669, 1432, 1515, 2371, 2749, 2920, 2960
MaeI	CTAG	3 cut(s)	453, 2964, 2990
MaeII	ACGT	5 cut(s)	276, 1201, 1223, 2329, 2497
MaeIII	GTNAC	9 cut(s)	143, 210, 289, 875, 952, 1331, 2325, 2883, 2889
MfeI	CAATTG	1 cut(s)	2895
MflI	RGATCY	7 cut(s)	4, 733, 1531, 1675, 2443, 2908, 2956
MhlI	GDGCHC	4 cut(s)	78, 276, 1327, 2301
MlsI	TGGCCA	2 cut(s)	373, 2041
MluNI	TGGCCA	2 cut(s)	373, 2041
MlyI	GAGTC	3 cut(s)	2041, 2846, 2983
MmeI	TCCRAC	2 cut(s)	1956, 2033
Mox20I	TGGCCA	2 cut(s)	373, 2041
MroXI	GAANNNNTTC	3 cut(s)	1165, 1215, 1490
MscI	TGGCCA	2 cut(s)	373, 2041
MseI	TTAA	9 cut(s)	794, 977, 1029, 1470, 1497, 2451, 2481, 2508, 2790
MslI	CAYNNNNRTG	3 cut(s)	158, 757, 1113
Msp20I	TGGCCA	2 cut(s)	373, 2041
MspA1I	CMGCKG	3 cut(s)	1790, 2570, 2654
MspI	CCGG	3 cut(s)	224, 544, 799
MspR9I	CCNGG	4 cut(s)	12, 1691, 1818, 2198
MunI	CAATTG	1 cut(s)	2895
Mva1269I	GAATGC	1 cut(s)	1766
MvaI	CCWGG	4 cut(s)	12, 1691, 1818, 2198
MvnI	CGCG	1 cut(s)	1307
NcoI	CCATGG	1 cut(s)	1170
NdeI	CATATG	1 cut(s)	1732
NlaIV	GGNNCC	8 cut(s)	300, 1353, 1728, 1883, 2100, 2462, 2910, 2958
NmeAIII	GCCGAG	2 cut(s)	97, 2788
NmuCI	GTSAC	3 cut(s)	210, 1331, 2889
NspI	RCATGY	3 cut(s)	1006, 1394, 3035
NspV	TTCGAA	1 cut(s)	925
PaeI	GCATGC	1 cut(s)	3035
PagI	TCATGA	2 cut(s)	217, 3098
PauI	GCGCGC	1 cut(s)	1305
PciI	ACATGT	1 cut(s)	1390
PcsI	WCGNNNNNNNCGW	1 cut(s)	2329
PctI	GAATGC	1 cut(s)	1766
PdmI	GAANNNNTTC	3 cut(s)	1165, 1215, 1490
PfeI	GAWTC	7 cut(s)	91, 445, 556, 634, 922, 1556, 1868
Pfl23II	CGTACG	1 cut(s)	2494
PinAI	ACCGGT	1 cut(s)	798
PleI	GAGTC	3 cut(s)	2040, 2846, 2982
PpsI	GAGTC	3 cut(s)	2040, 2846, 2982
Ppu21I	YACGTR	1 cut(s)	2498
PscI	ACATGT	1 cut(s)	1390
Psp6I	CCWGG	4 cut(s)	10, 1689, 1816, 2196
PspEI	GGTNACC	1 cut(s)	210
PspFI	CCCAGC	3 cut(s)	881, 2428, 2566
PspGI	CCWGG	4 cut(s)	10, 1689, 1816, 2196
PspLI	CGTACG	1 cut(s)	2494
PspN4I	GGNNCC	8 cut(s)	300, 1353, 1728, 1883, 2100, 2462, 2910, 2958
PspPI	GGNCC	2 cut(s)	2596, 2610
PsrI	GAACNNNNNNTAC	2 cut(s)	797, 829
PstI	CTGCAG	1 cut(s)	733
PstNI	CAGNNNCTG	2 cut(s)	737, 2657
PsuI	RGATCY	7 cut(s)	4, 733, 1531, 1675, 2443, 2908, 2956
PteI	GCGCGC	1 cut(s)	1305
PvuII	CAGCTG	3 cut(s)	1790, 2570, 2654
RsaI	GTAC	4 cut(s)	806, 1259, 2256, 2496
RsaNI	GTAC	4 cut(s)	805, 1258, 2255, 2495
RseI	CAYNNNNRTG	3 cut(s)	158, 757, 1113
SaqAI	TTAA	9 cut(s)	794, 977, 1029, 1470, 1497, 2451, 2481, 2508, 2790
Sau96I	GGNCC	2 cut(s)	2596, 2610
ScaI	AGTACT	1 cut(s)	2256
SchI	GAGTC	3 cut(s)	2041, 2846, 2983
ScrFI	CCNGG	4 cut(s)	12, 1691, 1818, 2198
SduI	GDGCHC	4 cut(s)	78, 276, 1327, 2301
SfaNI	GCATC	8 cut(s)	186, 669, 1432, 1515, 2371, 2749, 2920, 2960
SfcI	CTRYAG	3 cut(s)	729, 1698, 2073
SfuI	TTCGAA	1 cut(s)	925
SmiMI	CAYNNNNRTG	3 cut(s)	158, 757, 1113
SphI	GCATGC	1 cut(s)	3035
SsiI	CCGC	8 cut(s)	35, 69, 104, 1764, 2585, 2727, 2827, 2960
SspI	AATATT	2 cut(s)	974, 2092
SspMI	CTAG	3 cut(s)	453, 2964, 2990
StyD4I	CCNGG	4 cut(s)	10, 1689, 1816, 2196
StyI	CCWWGG	2 cut(s)	1170, 2963
TaaI	ACNGT	6 cut(s)	123, 813, 1154, 1458, 2325, 2889
TaiI	ACGT	5 cut(s)	279, 1204, 1226, 2332, 2500
TaqI	TCGA	6 cut(s)	925, 1291, 1549, 2120, 2332, 2844
TatI	WGTACW	2 cut(s)	804, 2254
TauI	GCSGC	3 cut(s)	38, 71, 1766
TfiI	GAWTC	7 cut(s)	91, 445, 556, 634, 922, 1556, 1868
Tru1I	TTAA	9 cut(s)	794, 977, 1029, 1470, 1497, 2451, 2481, 2508, 2790
Tru9I	TTAA	9 cut(s)	794, 977, 1029, 1470, 1497, 2451, 2481, 2508, 2790
TscAI	CASTG	8 cut(s)	374, 576, 720, 1189, 1566, 1878, 2294, 2875
TseFI	GTSAC	3 cut(s)	210, 1331, 2889
Tsp45I	GTSAC	3 cut(s)	210, 1331, 2889
TspGWI	ACGGA	3 cut(s)	76, 1364, 2932
TspRI	CASTG	8 cut(s)	374, 576, 720, 1189, 1566, 1878, 2294, 2875
XapI	RAATTY	5 cut(s)	606, 907, 1245, 1608, 2792
XbaI	TCTAGA	1 cut(s)	452
XceI	RCATGY	3 cut(s)	1006, 1394, 3035
XcmI	CCANNNNNNNNNTGG	2 cut(s)	419, 568
XmaJI	CCTAGG	1 cut(s)	2963
XmiI	GTMKAC	2 cut(s)	98, 1007
XmnI	GAANNNNTTC	3 cut(s)	1165, 1215, 1490
XspI	CTAG	3 cut(s)	453, 2964, 2990
ZrmI	AGTACT	1 cut(s)	2256

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.