Rh4BG376900

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003686
Physical Location & Seq
Forward (+)
53218800 .. 53233428
14629 bp
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UTR
Exon/CDS
Intron
Rh4BG376900.1

Sequence Viewer

Length: 723 bp
ATGTCCGACGAGGAGCACCACTTCGAATCTAAGGCCGACGCCGGAGCCTCCAAGACCTATCCTCAGCAGGCCGGCACCATCCGAAAGAACGGTTACATTGTCATCAAAAACAGGCCCTGCAAGGTTGTGGAGGTTTCCACTTCCAAAACCGGCAAGCACGGACATGCCAAGTGCCACTTTGTTGGTATTGACATCTTCACTGCCAAGAAGCTTGAGGATATTGTTCCCTCTTCCCACAATTGTGATGTTCCCCATGTCAACCGTACCGACTACCAGCTGATTGATATCTCTGAGGATGGATTTGTGAGTCTGCTGACTGAGAATGGTAACACCAAGGATGACCTGAGGCTTCCAACCGATGACAATCTGCTTACGCAGGTGCCTCCGCCTTTGAATTACCTTTCGGTGACTACTACAATGCTTGGAGGTGCAGCTGGAGCTGGTGCCTATGCGCTGGGTATGATATCTGATGCCTTCAGCTCCTTGGCTTTCACTGCTCTGGCTATTGTAGTTAGTGTTGCTGGAGCTATCGGTGTCGGATTTCCTGTTTTGGCTTGCACAGAAATGGTTATGCCAATGTCTAGTAGCCGAGATAGAAGCATGTTTCAAACATATGATTATAATTTCTCCTATTTTCAAGAGTGGTGCTGGAAGCAGTTCAATGTGAAACCAAGGCCTACATGGATAACAACAGAGTTTGGTGGACATTACTTTTGGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
eIF-5a

Protein Analysis

240

Amino Acids

26.47

Weight (kDa)

5.87

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF5A-like_N PF21485 17 - 79 3.8e-37 Translation initiation factor 5A-like, N-terminal
eIF-5a PF01287 85 - 126 1e-14 Eukaryotic elongation factor 5A hypusine, DNA-binding OB fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 621
AarI CACCTGC 1 cut(s) 367
Acc36I ACCTGC 1 cut(s) 367
AccB1I GGYRCC 3 cut(s) 74, 379, 443
AciI CCGC 1 cut(s) 386
AcuI CTGAAG 1 cut(s) 460
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 1 cut(s) 265
AgsI TTSAA 4 cut(s) 394, 608, 638, 661
AleI CACNNNNGTG 1 cut(s) 240
AluBI AGCT 6 cut(s) 211, 277, 434, 440, 480, 527
AluI AGCT 6 cut(s) 211, 277, 434, 440, 480, 527
Alw21I GWGCWC 1 cut(s) 18
AlwNI CAGNNNCTG 1 cut(s) 117
AoxI GGCC 5 cut(s) 33, 69, 113, 674, 716
ApeKI GCWGC 1 cut(s) 431
ArsI GACNNNNNNTTYG 1 cut(s) 696
Asp700I GAANNNNTTC 1 cut(s) 656
AspLEI GCGC 1 cut(s) 454
AspS9I GGNCC 1 cut(s) 114
AsuHPI GGTGA 1 cut(s) 418
AsuII TTCGAA 1 cut(s) 24
AxyI CCTNAGG 1 cut(s) 344
BanI GGYRCC 3 cut(s) 74, 379, 443
Bbv12I GWGCWC 1 cut(s) 18
BbvCI CCTCAGC 1 cut(s) 63
BbvI GCAGC 1 cut(s) 443
BccI CCATC 2 cut(s) 86, 290
BfaI CTAG 1 cut(s) 582
BfuAI ACCTGC 1 cut(s) 367
BisI GCNGC 1 cut(s) 432
BlsI GCNGC 1 cut(s) 433
BmgT120I GGNCC 1 cut(s) 114
BmiI GGNNCC 4 cut(s) 46, 76, 381, 445
BmsI GCATC 1 cut(s) 460
BpmI CTGGAG 2 cut(s) 456, 543
Bpu10I CCTNAGC 1 cut(s) 63
Bpu14I TTCGAA 1 cut(s) 24
BpuEI CTTGAG 1 cut(s) 233
BsaBI GATNNNNATC 2 cut(s) 284, 363
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 3 cut(s) 333, 483, 671
Bse118I RCCGGY 2 cut(s) 71, 149
Bse21I CCTNAGG 1 cut(s) 344
Bse8I GATNNNNATC 2 cut(s) 284, 363
BseDI CCNNGG 3 cut(s) 333, 483, 671
BseGI GGATG 3 cut(s) 78, 301, 343
BseJI GATNNNNATC 2 cut(s) 284, 363
BseMII CTCAG 4 cut(s) 77, 282, 309, 335
BseRI GAGGAG 1 cut(s) 26
BseXI GCAGC 1 cut(s) 443
BseYI CCCAGC 1 cut(s) 454
BsgI GTGCAG 1 cut(s) 450
BshFI GGCC 5 cut(s) 35, 71, 115, 676, 718
BshNI GGYRCC 3 cut(s) 74, 379, 443
BsiHKAI GWGCWC 1 cut(s) 18
BsiSI CCGG 3 cut(s) 42, 72, 150
BsnI GGCC 5 cut(s) 35, 71, 115, 676, 718
Bsp119I TTCGAA 1 cut(s) 24
Bsp1286I GDGCHC 1 cut(s) 18
BspACI CCGC 1 cut(s) 386
BspANI GGCC 5 cut(s) 35, 71, 115, 676, 718
BspCNI CTCAG 4 cut(s) 76, 283, 310, 336
BspLI GGNNCC 4 cut(s) 46, 76, 381, 445
BspMI ACCTGC 1 cut(s) 367
BspT104I TTCGAA 1 cut(s) 24
BspT107I GGYRCC 3 cut(s) 74, 379, 443
BsrFI RCCGGY 2 cut(s) 71, 149
BssAI RCCGGY 2 cut(s) 71, 149
BssECI CCNNGG 3 cut(s) 333, 483, 671
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 3 cut(s) 333, 483, 671
Bst4CI ACNGT 2 cut(s) 92, 263
Bst6I CTCTTC 1 cut(s) 235
BstACI GRCGYC 1 cut(s) 39
BstBI TTCGAA 1 cut(s) 24
BstC8I GCNNGC 4 cut(s) 69, 73, 155, 556
BstDEI CTNAG 5 cut(s) 30, 63, 291, 318, 344
BstF5I GGATG 3 cut(s) 78, 301, 343
BstHHI GCGC 1 cut(s) 454
BstMWI GCNNNNNNNGC 2 cut(s) 437, 494
BstNSI RCATGY 2 cut(s) 167, 604
BstV1I GCAGC 1 cut(s) 443
BstXI CCANNNNNNTGG 1 cut(s) 182
Bsu36I CCTNAGG 1 cut(s) 344
BsuRI GGCC 5 cut(s) 35, 71, 115, 676, 718
BtsCI GGATG 3 cut(s) 78, 301, 343
BtsI GCAGTG 2 cut(s) 198, 492
BtsIMutI CAGTG 2 cut(s) 198, 492
BveI ACCTGC 1 cut(s) 367
Cac8I GCNNGC 4 cut(s) 69, 73, 155, 556
CaiI CAGNNNCTG 1 cut(s) 117
CfoI GCGC 1 cut(s) 454
Cfr10I RCCGGY 2 cut(s) 71, 149
Cfr13I GGNCC 1 cut(s) 114
CseI GACGC 1 cut(s) 47
Csp6I GTAC 1 cut(s) 264
CviAII CATG 4 cut(s) 164, 254, 601, 681
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 5 cut(s) 30, 63, 291, 318, 344
Eam1104I CTCTTC 1 cut(s) 235
EarI CTCTTC 1 cut(s) 235
EciI GGCGGA 1 cut(s) 375
Eco130I CCWWGG 3 cut(s) 333, 483, 671
Eco147I AGGCCT 1 cut(s) 676
Eco32I GATATC 2 cut(s) 286, 465
Eco57I CTGAAG 1 cut(s) 460
Eco81I CCTNAGG 1 cut(s) 344
EcoO109I RGGNCCY 1 cut(s) 114
EcoRV GATATC 2 cut(s) 286, 465
EcoT14I CCWWGG 3 cut(s) 333, 483, 671
ErhI CCWWGG 3 cut(s) 333, 483, 671
FaeI CATG 4 cut(s) 167, 257, 604, 684
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FatI CATG 4 cut(s) 163, 253, 600, 680
FauNDI CATATG 1 cut(s) 613
Fnu4HI GCNGC 1 cut(s) 432
FokI GGATG 3 cut(s) 65, 308, 350
Fsp4HI GCNGC 1 cut(s) 432
FspBI CTAG 1 cut(s) 582
GlaI GCGC 1 cut(s) 453
GluI GCNGC 1 cut(s) 432
GsaI CCCAGC 1 cut(s) 458
GsuI CTGGAG 2 cut(s) 456, 543
HaeIII GGCC 5 cut(s) 35, 71, 115, 676, 718
HapII CCGG 3 cut(s) 42, 72, 150
HgaI GACGC 1 cut(s) 47
HhaI GCGC 1 cut(s) 454
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 4 cut(s) 167, 257, 604, 684
Hin6I GCGC 1 cut(s) 452
HinP1I GCGC 1 cut(s) 452
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 209
HinfI GANTC 2 cut(s) 26, 307
HpaII CCGG 3 cut(s) 42, 72, 150
HphI GGTGA 1 cut(s) 418
Hpy166II GTNNAC 2 cut(s) 259, 704
Hpy188I TCNGA 5 cut(s) 7, 83, 292, 469, 539
Hpy188III TCNNGA 1 cut(s) 638
Hpy8I GTNNAC 2 cut(s) 259, 704
Hpy99I CGWCG 2 cut(s) 11, 41
HpyAV CCTTC 1 cut(s) 484
HpyCH4III ACNGT 2 cut(s) 92, 263
HpyCH4V TGCA 3 cut(s) 120, 431, 558
HpyF10VI GCNNNNNNNGC 2 cut(s) 437, 494
HpyF3I CTNAG 5 cut(s) 30, 63, 291, 318, 344
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 4 cut(s) 167, 257, 604, 684
HspAI GCGC 1 cut(s) 452
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
LmnI GCTCC 5 cut(s) 13, 44, 437, 485, 524
Lsp1109I GCAGC 1 cut(s) 443
LweI GCATC 1 cut(s) 460
MaeI CTAG 1 cut(s) 582
MaeIII GTNAC 3 cut(s) 92, 326, 406
MboII GAAGA 2 cut(s) 187, 222
MfeI CAATTG 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 18
MluCI AATT 3 cut(s) 238, 394, 622
MlyI GAGTC 1 cut(s) 316
MmeI TCCRAC 3 cut(s) 30, 377, 517
MroNI GCCGGC 1 cut(s) 71
MroXI GAANNNNTTC 1 cut(s) 656
MseI TTAA 1 cut(s) 721
MslI CAYNNNNRTG 3 cut(s) 162, 240, 563
MspA1I CMGCKG 2 cut(s) 277, 434
MspI CCGG 3 cut(s) 42, 72, 150
MunI CAATTG 1 cut(s) 238
MwoI GCNNNNNNNGC 2 cut(s) 437, 494
NaeI GCCGGC 1 cut(s) 73
NdeI CATATG 1 cut(s) 613
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 4 cut(s) 167, 257, 604, 684
NlaIV GGNNCC 4 cut(s) 46, 76, 381, 445
NmeAIII GCCGAG 1 cut(s) 614
NmuCI GTSAC 1 cut(s) 406
NspI RCATGY 2 cut(s) 167, 604
NspV TTCGAA 1 cut(s) 24
OliI CACNNNNGTG 1 cut(s) 240
PaqCI CACCTGC 1 cut(s) 367
PceI AGGCCT 1 cut(s) 676
PdiI GCCGGC 1 cut(s) 73
PdmI GAANNNNTTC 1 cut(s) 656
PfeI GAWTC 1 cut(s) 26
PkrI GCNGC 1 cut(s) 433
PleI GAGTC 1 cut(s) 315
PpsI GAGTC 1 cut(s) 315
PsiI TTATAA 1 cut(s) 621
PspFI CCCAGC 1 cut(s) 454
PspN4I GGNNCC 4 cut(s) 46, 76, 381, 445
PspPI GGNCC 1 cut(s) 114
PstNI CAGNNNCTG 1 cut(s) 117
PvuII CAGCTG 2 cut(s) 277, 434
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
RseI CAYNNNNRTG 3 cut(s) 162, 240, 563
SaqAI TTAA 1 cut(s) 721
SatI GCNGC 1 cut(s) 432
Sau96I GGNCC 1 cut(s) 114
SchI GAGTC 1 cut(s) 316
SduI GDGCHC 1 cut(s) 18
SfaNI GCATC 1 cut(s) 460
SfuI TTCGAA 1 cut(s) 24
SmiMI CAYNNNNRTG 3 cut(s) 162, 240, 563
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 3 cut(s) 238, 394, 622
SseBI AGGCCT 1 cut(s) 676
SsiI CCGC 1 cut(s) 386
SspMI CTAG 1 cut(s) 582
StuI AGGCCT 1 cut(s) 676
StyI CCWWGG 3 cut(s) 333, 483, 671
TaaI ACNGT 2 cut(s) 92, 263
TaqI TCGA 1 cut(s) 24
TasI AATT 3 cut(s) 238, 394, 622
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 1 cut(s) 721
Tru9I TTAA 1 cut(s) 721
TscAI CASTG 2 cut(s) 205, 499
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 1 cut(s) 431
Tsp45I GTSAC 1 cut(s) 406
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 2 cut(s) 205, 499
XceI RCATGY 2 cut(s) 167, 604
XcmI CCANNNNNNNNNTGG 1 cut(s) 678
XmnI GAANNNNTTC 1 cut(s) 656
XspI CTAG 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.