Rorug05G0053900

Protein OSB2, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003609
Physical Location & Seq
Forward (+)
4700938 .. 4701264
327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0053900.1

Sequence Viewer

Length: 327 bp
ATGGTAGCGGCTAACGAGATATACCTCAAGGTTCCAAGTGGAGATGTATATCCAATGCAAGTGACAATGTGTAAAGGGCAGATGTGGGCAGAAAGAGGTTGGCCAGAATTTGCAAAAGTTTACTCCCTAGAACATGGATGCACCTTAGATTTTAATTACGAAGGCAAACATTCGCTTTTGAAAGTAGACCTTTATGATAGGACTAATTCGAAGATTAAATATGATGATCCATTAAGTTCAAGCAATGATGAACTTAATGATGAGGAACAGAATGTATCTGCAGGTATGTGTGTGAATTTGGTTATGTTTATGTCAATGTGTATTTAG

Protein Analysis

108

Amino Acids

12.32

Weight (kDa)

4.61

Isoelectric Point (pI)

18.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 5 - 67 1.7e-06 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 272
AccI GTMKAC 1 cut(s) 186
AciI CCGC 1 cut(s) 8
AclWI GGATC 1 cut(s) 221
AcoI YGGCCR 1 cut(s) 101
AcsI RAATTY 2 cut(s) 107, 295
AgsI TTSAA 2 cut(s) 181, 240
AlwI GGATC 1 cut(s) 221
AoxI GGCC 1 cut(s) 101
ApoI RAATTY 2 cut(s) 107, 295
AsuII TTCGAA 1 cut(s) 209
BalI TGGCCA 1 cut(s) 103
BfaI CTAG 1 cut(s) 128
BfmI CTRYAG 1 cut(s) 279
BfuAI ACCTGC 1 cut(s) 272
BisI GCNGC 1 cut(s) 9
BlsI GCNGC 1 cut(s) 10
BmiI GGNNCC 1 cut(s) 33
BmsI GCATC 1 cut(s) 128
Bpu14I TTCGAA 1 cut(s) 209
BpuEI CTTGAG 1 cut(s) 11
BsaBI GATNNNNATC 1 cut(s) 48
Bse3DI GCAATG 1 cut(s) 250
Bse8I GATNNNNATC 1 cut(s) 48
BseGI GGATG 1 cut(s) 143
BseJI GATNNNNATC 1 cut(s) 48
BseMI GCAATG 1 cut(s) 250
BshFI GGCC 1 cut(s) 103
BsnI GGCC 1 cut(s) 103
Bsp119I TTCGAA 1 cut(s) 209
Bsp143I GATC 1 cut(s) 226
BspACI CCGC 1 cut(s) 8
BspANI GGCC 1 cut(s) 103
BspLI GGNNCC 1 cut(s) 33
BspMAI CTGCAG 1 cut(s) 283
BspMI ACCTGC 1 cut(s) 272
BspPI GGATC 1 cut(s) 221
BspT104I TTCGAA 1 cut(s) 209
BsrDI GCAATG 1 cut(s) 250
BssMI GATC 1 cut(s) 226
BstBI TTCGAA 1 cut(s) 209
BstDEI CTNAG 1 cut(s) 145
BstF5I GGATG 1 cut(s) 143
BstKTI GATC 1 cut(s) 229
BstMBI GATC 1 cut(s) 226
BstSFI CTRYAG 1 cut(s) 279
BsuRI GGCC 1 cut(s) 103
BtsCI GGATG 1 cut(s) 143
BveI ACCTGC 1 cut(s) 272
CviAII CATG 1 cut(s) 134
CviJI RGCY 2 cut(s) 11, 103
CviKI_1 RGCY 2 cut(s) 11, 103
DdeI CTNAG 1 cut(s) 145
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 101
FaeI CATG 1 cut(s) 137
FaiI YATR 8 cut(s) 22, 49, 135, 195, 222, 287, 305, 311
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FatI CATG 1 cut(s) 133
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 1 cut(s) 9
FokI GGATG 1 cut(s) 150
Fsp4HI GCNGC 1 cut(s) 9
FspBI CTAG 1 cut(s) 128
GluI GCNGC 1 cut(s) 9
HaeIII GGCC 1 cut(s) 103
Hin1II CATG 1 cut(s) 137
Hpy166II GTNNAC 2 cut(s) 121, 187
Hpy8I GTNNAC 2 cut(s) 121, 187
HpyAV CCTTC 1 cut(s) 155
HpyCH4V TGCA 4 cut(s) 58, 113, 141, 281
HpyF3I CTNAG 1 cut(s) 145
Hsp92II CATG 1 cut(s) 137
Kzo9I GATC 1 cut(s) 226
LpnPI CCDG 2 cut(s) 117, 267
LweI GCATC 1 cut(s) 128
MaeI CTAG 1 cut(s) 128
MaeIII GTNAC 1 cut(s) 61
MalI GATC 1 cut(s) 228
MboI GATC 1 cut(s) 226
MboII GAAGA 1 cut(s) 223
MlsI TGGCCA 1 cut(s) 103
MluCI AATT 4 cut(s) 107, 154, 205, 295
MluNI TGGCCA 1 cut(s) 103
MnlI CCTC 3 cut(s) 35, 89, 256
Mox20I TGGCCA 1 cut(s) 103
MscI TGGCCA 1 cut(s) 103
MseI TTAA 4 cut(s) 153, 216, 233, 255
Msp20I TGGCCA 1 cut(s) 103
NdeII GATC 1 cut(s) 226
NlaIII CATG 1 cut(s) 137
NlaIV GGNNCC 1 cut(s) 33
NmuCI GTSAC 1 cut(s) 61
NspV TTCGAA 1 cut(s) 209
PkrI GCNGC 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 33
PstI CTGCAG 1 cut(s) 283
SaqAI TTAA 4 cut(s) 153, 216, 233, 255
SatI GCNGC 1 cut(s) 9
Sau3AI GATC 1 cut(s) 226
SetI ASST 6 cut(s) 27, 33, 100, 146, 192, 286
SfaNI GCATC 1 cut(s) 128
SfcI CTRYAG 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 209
SgeI CNNG 9 cut(s) 28, 40, 48, 71, 116, 140, 146, 252, 294
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 4 cut(s) 107, 154, 205, 295
SsiI CCGC 1 cut(s) 8
SspMI CTAG 1 cut(s) 128
TaqI TCGA 1 cut(s) 209
TasI AATT 4 cut(s) 107, 154, 205, 295
TauI GCSGC 1 cut(s) 11
Tru1I TTAA 4 cut(s) 153, 216, 233, 255
Tru9I TTAA 4 cut(s) 153, 216, 233, 255
TseFI GTSAC 1 cut(s) 61
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 1 cut(s) 264
XapI RAATTY 2 cut(s) 107, 295
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.