Rorug05G0273400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001727
Physical Location & Seq
Forward (+)
30867495 .. 30869453
1959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0273400.1

Sequence Viewer

Length: 600 bp
ATGTCGACAGTGCAGTATATGCGCCAGGATTCTGGGCCTCTGCCACTCCACAGCTTGATGAACTTCTTAAGCAAGCTACTCAACAGGTCAAAGATCTCAATAACTCATCACAAACTCTCTACCAAGCGTGGGTTGGTTCCAGCAGTTCGCCTAGAGTTGGGAAGCGGAGAATCTGATTTTGCAGCTTTTGTTCAAAATAATGGAATTCCGTCAGCCGACATGACTTTCTGTAAAGGATATCCAGTATACCATGCCATTCCATTGGCTAACTTATGGGGTTTGATAGCTCTTTGGCTAGCAAATGCAGAGTTTTTGCCTTTTGATTATACATCCTATGCGCTGGAGCTACAGAAATACATAAAGGATTTGGAAGTTGAAATCTTAGATAAGAACATTAACCTAACTCCTCTGGTCAAGTCCATTGAGGAGCTCAAAAAACCAGCCACAATAATCAACAACCAGAGAAAGGTCCAGAATTTAATTGAAAGGTGTCTGCAGTTGTGTATGAGCGGAGATGAAGTTGTCAATATCCTCTTAGATTGTGCTGGGATTGAGCCTGTGTTTACGAACTTGGGTATGGCAGTGTACTACTCCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VARLMGL

Protein Analysis

199

Amino Acids

22.32

Weight (kDa)

6.42

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_thal_3526 PF09713 157 - 191 3.4e-10 Plant protein 1589 of unknown function (A_thal_3526)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 510
AccI GTMKAC 2 cut(s) 5, 246
AciI CCGC 2 cut(s) 165, 510
AcsI RAATTY 2 cut(s) 204, 475
AfaI GTAC 1 cut(s) 587
AfiI CCNNNNNNNGG 3 cut(s) 129, 157, 466
AflII CTTAAG 1 cut(s) 67
AgsI TTSAA 3 cut(s) 194, 377, 485
AjnI CCWGG 1 cut(s) 24
AluBI AGCT 6 cut(s) 54, 76, 185, 287, 346, 430
AluI AGCT 6 cut(s) 54, 76, 185, 287, 346, 430
Alw21I GWGCWC 1 cut(s) 432
AoxI GGCC 1 cut(s) 35
ApeKI GCWGC 1 cut(s) 182
ApoI RAATTY 2 cut(s) 204, 475
AspLEI GCGC 2 cut(s) 24, 340
AspS9I GGNCC 2 cut(s) 35, 469
AsuNHI GCTAGC 1 cut(s) 295
AvaII GGWCC 1 cut(s) 469
BanII GRGCYC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 432
BbvI GCAGC 1 cut(s) 194
BciT130I CCWGG 1 cut(s) 26
BfaI CTAG 2 cut(s) 152, 296
BfmI CTRYAG 2 cut(s) 347, 494
BfrI CTTAAG 1 cut(s) 67
BglII AGATCT 1 cut(s) 93
BisI GCNGC 1 cut(s) 183
BlsI GCNGC 1 cut(s) 184
Bme1390I CCNGG 1 cut(s) 26
Bme18I GGWCC 1 cut(s) 469
BmgT120I GGNCC 2 cut(s) 35, 469
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 1 cut(s) 26
BmtI GCTAGC 1 cut(s) 299
BpmI CTGGAG 1 cut(s) 362
BsaXI ACNNNNNCTCC 2 cut(s) 504, 534
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 157, 466
Bse1I ACTGG 1 cut(s) 242
BseBI CCWGG 1 cut(s) 26
BseGI GGATG 1 cut(s) 329
BseLI CCNNNNNNNGG 3 cut(s) 129, 157, 466
BseNI ACTGG 1 cut(s) 242
BseRI GAGGAG 2 cut(s) 396, 440
BseXI GCAGC 1 cut(s) 194
BseYI CCCAGC 1 cut(s) 545
BsgI GTGCAG 1 cut(s) 32
BshFI GGCC 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 432
BslI CCNNNNNNNGG 3 cut(s) 129, 157, 466
BsnI GGCC 1 cut(s) 37
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 1 cut(s) 93
BspACI CCGC 2 cut(s) 165, 510
BspANI GGCC 1 cut(s) 37
BspLI GGNNCC 1 cut(s) 138
BspMAI CTGCAG 1 cut(s) 498
BspOI GCTAGC 1 cut(s) 299
BspTI CTTAAG 1 cut(s) 67
BsrBI CCGCTC 1 cut(s) 510
BsrI ACTGG 1 cut(s) 242
BssMI GATC 1 cut(s) 93
BssNAI GTATAC 1 cut(s) 247
Bst1107I GTATAC 1 cut(s) 247
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 1 cut(s) 10
BstAFI CTTAAG 1 cut(s) 67
BstAPI GCANNNNNTGC 1 cut(s) 19
BstC8I GCNNGC 2 cut(s) 74, 297
BstDEI CTNAG 2 cut(s) 382, 535
BstF5I GGATG 1 cut(s) 329
BstHHI GCGC 2 cut(s) 24, 340
BstKTI GATC 1 cut(s) 96
BstMBI GATC 1 cut(s) 93
BstMWI GCNNNNNNNGC 1 cut(s) 19
BstNI CCWGG 1 cut(s) 26
BstSCI CCNGG 1 cut(s) 24
BstSFI CTRYAG 2 cut(s) 347, 494
BstV1I GCAGC 1 cut(s) 194
BstX2I RGATCY 1 cut(s) 93
BstXI CCANNNNNNTGG 2 cut(s) 32, 262
BstYI RGATCY 1 cut(s) 93
BstZ17I GTATAC 1 cut(s) 247
BsuRI GGCC 1 cut(s) 37
BtsCI GGATG 1 cut(s) 329
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 2 cut(s) 15, 588
Cac8I GCNNGC 2 cut(s) 74, 297
CfoI GCGC 2 cut(s) 24, 340
Cfr13I GGNCC 2 cut(s) 35, 469
Csp6I GTAC 1 cut(s) 586
CviAII CATG 2 cut(s) 220, 251
CviQI GTAC 1 cut(s) 586
DdeI CTNAG 2 cut(s) 382, 535
DpnI GATC 1 cut(s) 95
DpnII GATC 1 cut(s) 93
Ecl136II GAGCTC 1 cut(s) 430
Eco24I GRGCYC 1 cut(s) 432
Eco32I GATATC 1 cut(s) 239
Eco47I GGWCC 1 cut(s) 469
Eco53kI GAGCTC 1 cut(s) 430
EcoICRI GAGCTC 1 cut(s) 430
EcoRI GAATTC 1 cut(s) 204
EcoRII CCWGG 1 cut(s) 24
EcoRV GATATC 1 cut(s) 239
EcoT38I GRGCYC 1 cut(s) 432
FaeI CATG 2 cut(s) 223, 254
FatI CATG 2 cut(s) 219, 250
FblI GTMKAC 2 cut(s) 5, 246
Fnu4HI GCNGC 1 cut(s) 183
FokI GGATG 1 cut(s) 316
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 1 cut(s) 183
FspBI CTAG 2 cut(s) 152, 296
GlaI GCGC 2 cut(s) 23, 339
GluI GCNGC 1 cut(s) 183
GsaI CCCAGC 1 cut(s) 549
GsuI CTGGAG 1 cut(s) 362
HaeIII GGCC 1 cut(s) 37
HhaI GCGC 2 cut(s) 24, 340
Hin1II CATG 2 cut(s) 223, 254
Hin6I GCGC 2 cut(s) 22, 338
HinP1I GCGC 2 cut(s) 22, 338
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 2 cut(s) 29, 170
Hpy166II GTNNAC 4 cut(s) 6, 247, 564, 586
Hpy188I TCNGA 1 cut(s) 175
Hpy188III TCNNGA 1 cut(s) 472
Hpy8I GTNNAC 4 cut(s) 6, 247, 564, 586
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4V TGCA 4 cut(s) 13, 182, 305, 496
HpyF10VI GCNNNNNNNGC 1 cut(s) 19
HpyF3I CTNAG 2 cut(s) 382, 535
Hsp92II CATG 2 cut(s) 223, 254
HspAI GCGC 2 cut(s) 22, 338
Kzo9I GATC 1 cut(s) 93
LmnI GCTCC 2 cut(s) 343, 427
Lsp1109I GCAGC 1 cut(s) 194
MaeI CTAG 2 cut(s) 152, 296
MalI GATC 1 cut(s) 95
MbiI CCGCTC 1 cut(s) 510
MboI GATC 1 cut(s) 93
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 3 cut(s) 204, 475, 480
MnlI CCTC 4 cut(s) 48, 417, 418, 542
MseI TTAA 3 cut(s) 68, 396, 479
MspCI CTTAAG 1 cut(s) 67
MspR9I CCNGG 1 cut(s) 26
MvaI CCWGG 1 cut(s) 26
MwoI GCNNNNNNNGC 1 cut(s) 19
NdeII GATC 1 cut(s) 93
NheI GCTAGC 1 cut(s) 295
NlaIII CATG 2 cut(s) 223, 254
NlaIV GGNNCC 1 cut(s) 138
PfeI GAWTC 2 cut(s) 29, 170
PkrI GCNGC 1 cut(s) 184
Psp124BI GAGCTC 1 cut(s) 432
Psp6I CCWGG 1 cut(s) 24
PspFI CCCAGC 1 cut(s) 545
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 138
PspPI GGNCC 2 cut(s) 35, 469
PstI CTGCAG 1 cut(s) 498
PsuI RGATCY 1 cut(s) 93
RsaI GTAC 1 cut(s) 587
RsaNI GTAC 1 cut(s) 586
SacI GAGCTC 1 cut(s) 432
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 3 cut(s) 68, 396, 479
SatI GCNGC 1 cut(s) 183
Sau3AI GATC 1 cut(s) 93
Sau96I GGNCC 2 cut(s) 35, 469
ScrFI CCNGG 1 cut(s) 26
SduI GDGCHC 1 cut(s) 432
SfcI CTRYAG 2 cut(s) 347, 494
SinI GGWCC 1 cut(s) 469
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
Sse9I AATT 3 cut(s) 204, 475, 480
SsiI CCGC 2 cut(s) 165, 510
SspMI CTAG 2 cut(s) 152, 296
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 1 cut(s) 24
TaaI ACNGT 1 cut(s) 10
TaqI TCGA 1 cut(s) 5
TasI AATT 3 cut(s) 204, 475, 480
TatI WGTACW 1 cut(s) 585
TfiI GAWTC 2 cut(s) 29, 170
Tru1I TTAA 3 cut(s) 68, 396, 479
Tru9I TTAA 3 cut(s) 68, 396, 479
TscAI CASTG 2 cut(s) 15, 588
TseI GCWGC 1 cut(s) 182
TspDTI ATGAA 2 cut(s) 74, 531
TspGWI ACGGA 1 cut(s) 198
TspRI CASTG 2 cut(s) 15, 588
Vha464I CTTAAG 1 cut(s) 67
VpaK11BI GGWCC 1 cut(s) 469
XapI RAATTY 2 cut(s) 204, 475
XcmI CCANNNNNNNNNTGG 1 cut(s) 130
XmiI GTMKAC 2 cut(s) 5, 246
XspI CTAG 2 cut(s) 152, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.