Rorug06G0450200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002780
Physical Location & Seq
Forward (+)
57988907 .. 57991760
2854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0450200.1

Sequence Viewer

Length: 1296 bp
ATGACCACTTCACCCAAAACCCACATGATCAACATCAACTCCAGCAGCAATGACTCAGATCAGCCAAACAAACCCTTACTTCAAAAATCCCCAAAACCCTCCGACGAGCTATCCAACTTCCAAATCAGCCTCAAATGGTGCGCACTCGACCACTCCACGTACCTGGGAAAGTTTATCTCCTACTTCACCTTCATCCTCCTGACCCTCGTCGTCCCAATCCTAACTTCTCTCACACTTAAGCTTCCAACCAATGACTACCCCATTTCCTTTAACAAACTAGTCCAGCTCCCCGAATCCGGGTTATCTGCCATTGCCTTCTTCACACTCTCCGCCTTCTTTCGGAATTACGGCCTCAAGCAGCTCCTCTTCCTTGATGGCCTCGAAGACGATACTTTCTCTGTCCGGAGGGGATACACCCGCGAGGTCGATAAGGCCTTCAAGTGCTTGGTATGCATACTCTTGCCTTCGTTCTTCATCGAGATTGCTCACAAGATTCTATTCTTCACCACCGTGAAAGTGTCACTGCCATATATGAGTCCGAGTGTCCCGTTGAATTCCATTGTGTTTGTTTTGGTGTTGGCTTCGTGGGTTTATCGGACCGGGGTGTTCTTGCTGGTTTGTATTCTCTTCCGATTGACATGTGAGCTCCAAATACTTCGGTTCGAAGGGTTGCATAAGTTCTTTGAAGGGTCTGATTCCAATTCTAGAGTCATTTTTCAGGAACATGTGAGGATAAGAAAGCAATTGTGGGTTACGAGCCACAGGTACAGATTCTTTATCATTGGGTGCTGGTTTACCAGCACCGTCAGCCAATTTGCAGCCTTGTTGATGGTTCTGGCATCCAAGGCAGAGAAGACTTTTCTCAACTCCGGTGACCTTGTGGTTTGCTCGGCTGTTGAGCTAAGTGGGTTGTTTTTGTGCTTAATGGAGGCAGCAAGGATGACACATAGAGCTCAAGGAATTGTTGCAATTGCTACAAGATGGCATATGCTTTTAACTTGTTCATCTGCTACTGGGTTAGATTCCGATAAGCAGGGGAAAGGCCAGAGCCCTGAGGCTAATGGAGGCTGTGATGGAGAGAGTGACAATGATTCATCGGACATTTCCACTTCTGTTTCTCCACATGACCCTTCCTCGTTCCAAACCAGGCAAGCTTTAGTGACATACTTGGGACACAACCATGGAGGGATCACGCTGTTCGGATTTGCACTTGATCGAGGGATGCTTCACACAATATTCGCCTTCGAGTTCTCTCTGGTGCTATGGATACTCAGTAAAGTAGTTGTTTTGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

431

Amino Acids

48.24

Weight (kDa)

8.27

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 35 - 415 2.9e-141 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 142
AccII CGCG 1 cut(s) 420
AccIII TCCGGA 1 cut(s) 402
AciI CCGC 2 cut(s) 330, 418
AclWI GGATC 1 cut(s) 1196
AcsI RAATTY 1 cut(s) 553
AfaI GTAC 2 cut(s) 161, 767
AfiI CCNNNNNNNGG 3 cut(s) 296, 297, 339
AflII CTTAAG 1 cut(s) 236
AflIII ACRYGT 2 cut(s) 638, 724
AgsI TTSAA 4 cut(s) 83, 439, 553, 686
AhlI ACTAGT 1 cut(s) 277
AjnI CCWGG 2 cut(s) 162, 1145
AjuI GAANNNNNNNTTGG 2 cut(s) 208, 240
AleI CACNNNNGTG 1 cut(s) 509
AluBI AGCT 8 cut(s) 109, 241, 286, 361, 646, 901, 953, 1154
AluI AGCT 8 cut(s) 109, 241, 286, 361, 646, 901, 953, 1154
Alw21I GWGCWC 2 cut(s) 648, 955
AlwI GGATC 1 cut(s) 1196
Aor13HI TCCGGA 1 cut(s) 402
AoxI GGCC 4 cut(s) 349, 376, 432, 1042
ApeKI GCWGC 4 cut(s) 45, 358, 818, 932
ApoI RAATTY 1 cut(s) 553
AspLEI GCGC 1 cut(s) 143
AspS9I GGNCC 1 cut(s) 597
AsuC2I CCSGG 2 cut(s) 298, 601
AsuHPI GGTGA 4 cut(s) 3, 178, 496, 884
AsuII TTCGAA 1 cut(s) 663
AvaII GGWCC 1 cut(s) 597
AxyI CCTNAGG 1 cut(s) 1053
BanII GRGCYC 3 cut(s) 648, 955, 1052
BbsI GAAGAC 2 cut(s) 390, 860
Bbv12I GWGCWC 2 cut(s) 648, 955
BbvI GCAGC 4 cut(s) 57, 370, 830, 944
BccI CCATC 4 cut(s) 368, 823, 975, 1067
BceAI ACGGC 1 cut(s) 364
BciT130I CCWGG 2 cut(s) 164, 1147
BciVI GTATCC 2 cut(s) 404, 1260
BclI TGATCA 1 cut(s) 27
BcnI CCSGG 2 cut(s) 298, 601
BcuI ACTAGT 1 cut(s) 277
BfaI CTAG 2 cut(s) 278, 705
BfrI CTTAAG 1 cut(s) 236
BfuI GTATCC 2 cut(s) 404, 1260
BisI GCNGC 4 cut(s) 46, 359, 819, 933
BlsI GCNGC 4 cut(s) 47, 360, 820, 934
Bme1390I CCNGG 4 cut(s) 164, 298, 601, 1147
Bme18I GGWCC 1 cut(s) 597
BmgT120I GGNCC 1 cut(s) 597
BmrFI CCNGG 4 cut(s) 164, 298, 601, 1147
BmrI ACTGGG 1 cut(s) 1023
BmsI GCATC 2 cut(s) 848, 1212
BmuI ACTGGG 1 cut(s) 1023
BoxI GACNNNNGTC 1 cut(s) 206
BpiI GAAGAC 2 cut(s) 390, 860
BpmI CTGGAG 1 cut(s) 25
Bpu14I TTCGAA 1 cut(s) 663
BpuEI CTTGAG 2 cut(s) 338, 939
BpuMI CCSGG 2 cut(s) 298, 601
BsaAI YACGTR 1 cut(s) 159
BsaBI GATNNNNATC 1 cut(s) 32
BsaJI CCNNGG 4 cut(s) 163, 600, 843, 1180
BsaWI WCCGGW 2 cut(s) 402, 869
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 3 cut(s) 296, 297, 339
Bse1I ACTGG 1 cut(s) 1018
Bse21I CCTNAGG 1 cut(s) 1053
Bse3DI GCAATG 2 cut(s) 55, 309
Bse8I GATNNNNATC 1 cut(s) 32
BseAI TCCGGA 1 cut(s) 402
BseBI CCWGG 2 cut(s) 164, 1147
BseDI CCNNGG 4 cut(s) 163, 600, 843, 1180
BseGI GGATG 4 cut(s) 192, 839, 945, 1227
BseJI GATNNNNATC 1 cut(s) 32
BseLI CCNNNNNNNGG 3 cut(s) 296, 297, 339
BseMI GCAATG 2 cut(s) 55, 309
BseMII CTCAG 3 cut(s) 69, 1044, 1285
BseNI ACTGG 1 cut(s) 1018
BseRI GAGGAG 1 cut(s) 353
BseXI GCAGC 4 cut(s) 57, 370, 830, 944
Bsh1236I CGCG 1 cut(s) 420
BshFI GGCC 4 cut(s) 351, 378, 434, 1044
BsiHKAI GWGCWC 2 cut(s) 648, 955
BsiSI CCGG 4 cut(s) 297, 403, 600, 870
BslFI GGGAC 3 cut(s) 197, 530, 1185
BslI CCNNNNNNNGG 3 cut(s) 296, 297, 339
BsmFI GGGAC 3 cut(s) 197, 530, 1185
BsnI GGCC 4 cut(s) 351, 378, 434, 1044
Bsp119I TTCGAA 1 cut(s) 663
Bsp1286I GDGCHC 3 cut(s) 648, 955, 1052
Bsp13I TCCGGA 1 cut(s) 402
Bsp143I GATC 4 cut(s) 27, 58, 1188, 1213
Bsp19I CCATGG 1 cut(s) 1180
BspACI CCGC 2 cut(s) 330, 418
BspANI GGCC 4 cut(s) 351, 378, 434, 1044
BspCNI CTCAG 3 cut(s) 68, 1045, 1284
BspEI TCCGGA 1 cut(s) 402
BspFNI CGCG 1 cut(s) 420
BspPI GGATC 1 cut(s) 1196
BspT104I TTCGAA 1 cut(s) 663
BspTI CTTAAG 1 cut(s) 236
BsrDI GCAATG 2 cut(s) 55, 309
BsrI ACTGG 1 cut(s) 1018
BssECI CCNNGG 4 cut(s) 163, 600, 843, 1180
BssMI GATC 4 cut(s) 27, 58, 1188, 1213
BssT1I CCWWGG 2 cut(s) 843, 1180
Bst2UI CCWGG 2 cut(s) 164, 1147
Bst4CI ACNGT 2 cut(s) 511, 805
Bst6I CTCTTC 2 cut(s) 371, 632
BstAFI CTTAAG 1 cut(s) 236
BstBAI YACGTR 1 cut(s) 159
BstBI TTCGAA 1 cut(s) 663
BstC8I GCNNGC 1 cut(s) 1152
BstDEI CTNAG 4 cut(s) 55, 902, 1053, 1271
BstDSI CCRYGG 1 cut(s) 1180
BstEII GGTNACC 1 cut(s) 872
BstF5I GGATG 4 cut(s) 192, 839, 945, 1227
BstFNI CGCG 1 cut(s) 420
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 4 cut(s) 30, 61, 1191, 1216
BstMBI GATC 4 cut(s) 27, 58, 1188, 1213
BstMWI GCNNNNNNNGC 3 cut(s) 450, 807, 845
BstNI CCWGG 2 cut(s) 164, 1147
BstNSI RCATGY 2 cut(s) 642, 728
BstPAI GACNNNNGTC 1 cut(s) 206
BstPI GGTNACC 1 cut(s) 872
BstSCI CCNGG 4 cut(s) 162, 296, 599, 1145
BstUI CGCG 1 cut(s) 420
BstV1I GCAGC 4 cut(s) 57, 370, 830, 944
BstV2I GAAGAC 2 cut(s) 390, 860
BstXI CCANNNNNNTGG 1 cut(s) 163
Bsu36I CCTNAGG 1 cut(s) 1053
BsuI GTATCC 2 cut(s) 404, 1260
BsuRI GGCC 4 cut(s) 351, 378, 434, 1044
BtgI CCRYGG 1 cut(s) 1180
BtsCI GGATG 4 cut(s) 192, 839, 945, 1227
BtsI GCAGTG 1 cut(s) 521
BtsIMutI CAGTG 1 cut(s) 521
Cac8I GCNNGC 1 cut(s) 1152
CfoI GCGC 1 cut(s) 143
Cfr13I GGNCC 1 cut(s) 597
CpoI CGGWCCG 1 cut(s) 597
Csp6I GTAC 2 cut(s) 160, 766
CspI CGGWCCG 1 cut(s) 597
CviAII CATG 5 cut(s) 25, 639, 725, 1124, 1181
CviQI GTAC 2 cut(s) 160, 766
DdeI CTNAG 4 cut(s) 55, 902, 1053, 1271
DpnI GATC 4 cut(s) 29, 60, 1190, 1215
DpnII GATC 4 cut(s) 27, 58, 1188, 1213
Eam1104I CTCTTC 2 cut(s) 371, 632
EarI CTCTTC 2 cut(s) 371, 632
EciI GGCGGA 1 cut(s) 319
Ecl136II GAGCTC 2 cut(s) 646, 953
Eco130I CCWWGG 2 cut(s) 843, 1180
Eco147I AGGCCT 1 cut(s) 434
Eco24I GRGCYC 3 cut(s) 648, 955, 1052
Eco47I GGWCC 1 cut(s) 597
Eco53kI GAGCTC 2 cut(s) 646, 953
Eco81I CCTNAGG 1 cut(s) 1053
Eco91I GGTNACC 1 cut(s) 872
EcoICRI GAGCTC 2 cut(s) 646, 953
EcoO65I GGTNACC 1 cut(s) 872
EcoRI GAATTC 1 cut(s) 553
EcoRII CCWGG 2 cut(s) 162, 1145
EcoT14I CCWWGG 2 cut(s) 843, 1180
EcoT22I ATGCAT 1 cut(s) 455
EcoT38I GRGCYC 3 cut(s) 648, 955, 1052
ErhI CCWWGG 2 cut(s) 843, 1180
FaeI CATG 5 cut(s) 28, 642, 728, 1127, 1184
FaqI GGGAC 3 cut(s) 197, 530, 1185
FatI CATG 5 cut(s) 24, 638, 724, 1123, 1180
FauI CCCGC 1 cut(s) 425
FauNDI CATATG 1 cut(s) 987
FbaI TGATCA 1 cut(s) 27
Fnu4HI GCNGC 4 cut(s) 46, 359, 819, 933
FokI GGATG 4 cut(s) 179, 826, 952, 1234
FriOI GRGCYC 3 cut(s) 648, 955, 1052
Fsp4HI GCNGC 4 cut(s) 46, 359, 819, 933
FspAI RTGCGCAY 1 cut(s) 142
FspBI CTAG 2 cut(s) 278, 705
FspI TGCGCA 1 cut(s) 142
GlaI GCGC 1 cut(s) 142
GluI GCNGC 4 cut(s) 46, 359, 819, 933
GsuI CTGGAG 1 cut(s) 25
HaeIII GGCC 4 cut(s) 351, 378, 434, 1044
HapII CCGG 4 cut(s) 297, 403, 600, 870
HhaI GCGC 1 cut(s) 143
Hin1II CATG 5 cut(s) 28, 642, 728, 1127, 1184
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HindIII AAGCTT 2 cut(s) 239, 1152
HinfI GANTC 9 cut(s) 53, 293, 493, 535, 695, 708, 771, 1022, 1091
HpaII CCGG 4 cut(s) 297, 403, 600, 870
HphI GGTGA 4 cut(s) 3, 178, 496, 884
Hpy166II GTNNAC 1 cut(s) 795
Hpy188III TCNNGA 5 cut(s) 199, 403, 478, 705, 719
Hpy8I GTNNAC 1 cut(s) 795
Hpy99I CGWCG 2 cut(s) 107, 212
HpyAV CCTTC 9 cut(s) 199, 325, 343, 445, 474, 659, 680, 1140, 1252
HpyCH4III ACNGT 2 cut(s) 511, 805
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 5 cut(s) 453, 673, 818, 968, 1208
HpyF10VI GCNNNNNNNGC 3 cut(s) 450, 807, 845
HpyF3I CTNAG 4 cut(s) 55, 902, 1053, 1271
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 5 cut(s) 28, 642, 728, 1127, 1184
HspAI GCGC 1 cut(s) 141
Kpn2I TCCGGA 1 cut(s) 402
Ksp22I TGATCA 1 cut(s) 27
Kzo9I GATC 4 cut(s) 27, 58, 1188, 1213
LmnI GCTCC 3 cut(s) 291, 366, 651
Lsp1109I GCAGC 4 cut(s) 57, 370, 830, 944
LweI GCATC 2 cut(s) 848, 1212
MaeI CTAG 2 cut(s) 278, 705
MaeII ACGT 1 cut(s) 158
MaeIII GTNAC 5 cut(s) 519, 751, 872, 1082, 1159
MalI GATC 4 cut(s) 29, 60, 1190, 1215
MboI GATC 4 cut(s) 27, 58, 1188, 1213
MboII GAAGA 7 cut(s) 310, 358, 395, 463, 493, 619, 865
MfeI CAATTG 2 cut(s) 743, 969
MhlI GDGCHC 3 cut(s) 648, 955, 1052
MluCI AATT 7 cut(s) 343, 553, 700, 743, 812, 960, 969
MlyI GAGTC 3 cut(s) 47, 544, 717
MmeI TCCRAC 3 cut(s) 126, 138, 269
Mph1103I ATGCAT 1 cut(s) 455
MroI TCCGGA 1 cut(s) 402
MseI TTAA 5 cut(s) 237, 270, 923, 995, 1294
MslI CAYNNNNRTG 2 cut(s) 509, 1179
MspCI CTTAAG 1 cut(s) 236
MspI CCGG 4 cut(s) 297, 403, 600, 870
MspR9I CCNGG 4 cut(s) 164, 298, 601, 1147
MunI CAATTG 2 cut(s) 743, 969
MvaI CCWGG 2 cut(s) 164, 1147
MvnI CGCG 1 cut(s) 420
MwoI GCNNNNNNNGC 3 cut(s) 450, 807, 845
NciI CCSGG 2 cut(s) 298, 601
NcoI CCATGG 1 cut(s) 1180
NdeI CATATG 1 cut(s) 987
NdeII GATC 4 cut(s) 27, 58, 1188, 1213
NlaIII CATG 5 cut(s) 28, 642, 728, 1127, 1184
NmeAIII GCCGAG 1 cut(s) 869
NmuCI GTSAC 4 cut(s) 519, 872, 1082, 1159
NsbI TGCGCA 1 cut(s) 142
NsiI ATGCAT 1 cut(s) 455
NspI RCATGY 2 cut(s) 642, 728
NspV TTCGAA 1 cut(s) 663
OliI CACNNNNGTG 1 cut(s) 509
PceI AGGCCT 1 cut(s) 434
PciI ACATGT 2 cut(s) 638, 724
PfeI GAWTC 6 cut(s) 293, 493, 695, 771, 1022, 1091
PkrI GCNGC 4 cut(s) 47, 360, 820, 934
PleI GAGTC 3 cut(s) 47, 543, 716
PpsI GAGTC 3 cut(s) 47, 543, 716
Ppu21I YACGTR 1 cut(s) 159
PscI ACATGT 2 cut(s) 638, 724
PshAI GACNNNNGTC 1 cut(s) 206
Psp124BI GAGCTC 2 cut(s) 648, 955
Psp6I CCWGG 2 cut(s) 162, 1145
PspEI GGTNACC 1 cut(s) 872
PspGI CCWGG 2 cut(s) 162, 1145
PspPI GGNCC 1 cut(s) 597
RsaI GTAC 2 cut(s) 161, 767
RsaNI GTAC 2 cut(s) 160, 766
RseI CAYNNNNRTG 2 cut(s) 509, 1179
Rsr2I CGGWCCG 1 cut(s) 597
RsrII CGGWCCG 1 cut(s) 597
SacI GAGCTC 2 cut(s) 648, 955
SaqAI TTAA 5 cut(s) 237, 270, 923, 995, 1294
SatI GCNGC 4 cut(s) 46, 359, 819, 933
Sau3AI GATC 4 cut(s) 27, 58, 1188, 1213
Sau96I GGNCC 1 cut(s) 597
SchI GAGTC 3 cut(s) 47, 544, 717
ScrFI CCNGG 4 cut(s) 164, 298, 601, 1147
SduI GDGCHC 3 cut(s) 648, 955, 1052
SfaNI GCATC 2 cut(s) 848, 1212
SfuI TTCGAA 1 cut(s) 663
SinI GGWCC 1 cut(s) 597
SmiMI CAYNNNNRTG 2 cut(s) 509, 1179
SmlI CTYRAG 3 cut(s) 236, 353, 954
SmoI CTYRAG 3 cut(s) 236, 353, 954
SpeI ACTAGT 1 cut(s) 277
Sse9I AATT 7 cut(s) 343, 553, 700, 743, 812, 960, 969
SseBI AGGCCT 1 cut(s) 434
SsiI CCGC 2 cut(s) 330, 418
SspI AATATT 1 cut(s) 1236
SspMI CTAG 2 cut(s) 278, 705
SstI GAGCTC 2 cut(s) 648, 955
StuI AGGCCT 1 cut(s) 434
StyD4I CCNGG 4 cut(s) 162, 296, 599, 1145
StyI CCWWGG 2 cut(s) 843, 1180
TaaI ACNGT 2 cut(s) 511, 805
TaiI ACGT 1 cut(s) 161
TaqI TCGA 7 cut(s) 147, 381, 426, 477, 663, 1216, 1245
TasI AATT 7 cut(s) 343, 553, 700, 743, 812, 960, 969
TfiI GAWTC 6 cut(s) 293, 493, 695, 771, 1022, 1091
Tru1I TTAA 5 cut(s) 237, 270, 923, 995, 1294
Tru9I TTAA 5 cut(s) 237, 270, 923, 995, 1294
TscAI CASTG 1 cut(s) 528
TseFI GTSAC 4 cut(s) 519, 872, 1082, 1159
TseI GCWGC 4 cut(s) 45, 358, 818, 932
Tsp45I GTSAC 4 cut(s) 519, 872, 1082, 1159
TspDTI ATGAA 4 cut(s) 181, 463, 993, 1083
TspRI CASTG 1 cut(s) 528
Vha464I CTTAAG 1 cut(s) 236
VpaK11BI GGWCC 1 cut(s) 597
XapI RAATTY 1 cut(s) 553
XbaI TCTAGA 1 cut(s) 704
XceI RCATGY 2 cut(s) 642, 728
XspI CTAG 2 cut(s) 278, 705
Zsp2I ATGCAT 1 cut(s) 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.