Rh4BG092700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002780
Physical Location & Seq
Reverse (-)
15886186 .. 15887732
1547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG092700.1

Sequence Viewer

Length: 543 bp
ATGGAAGCTCTAAACCCAGCAACCCCTAACACTCCGAAATCGACGAACCCGAATCCCCAGACTCCGACCCAAAGCCCATCATCGTCTGCTCGGCTGTGGAGGCCCACCGCGCAGCGCAACCTGAGACACCAGTGGTCCAAGTTAGCTTCTTTGAGACAACAATGGGAGTCTGCTTCTTCCAGCGGAAGAGCACACGCCACTTCGCTCGTAAACTCCTACCTTTCCATCAAGGACATGCCTTCAAGGGAGTTGGGGGTTCTCAGCGACATGCCGGATATACTAAAGAAAGCTTCACAGAAATTGCTTAAGCAGCAGGAACTTTATCGGAGCAAGCTTTTATCATCATACAAGGACATGGTGTCTGTTGTAACTAATATGGTCAAAACTAGTAGATCCATGAGATGCTTTGTCAAAGGGTCAACCAATAGTCCACTTTTACAATTTTCTGGCTATTCCGAATGTAATAATGACCCTGGAGATGGTGGTGGGATTCTAGTCTTCACATTTTGGTCTATCTCTTGCTTTGGTACATCTACAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

180

Amino Acids

19.89

Weight (kDa)

9.86

Isoelectric Point (pI)

52.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 110
AciI CCGC 2 cut(s) 108, 183
AclWI GGATC 1 cut(s) 387
AfaI GTAC 1 cut(s) 529
AfiI CCNNNNNNNGG 1 cut(s) 479
AflII CTTAAG 1 cut(s) 305
AgsI TTSAA 1 cut(s) 243
AhdI GACNNNNNGTC 1 cut(s) 358
AhlI ACTAGT 1 cut(s) 386
AjnI CCWGG 1 cut(s) 472
AluBI AGCT 4 cut(s) 8, 146, 290, 334
AluI AGCT 4 cut(s) 8, 146, 290, 334
Alw21I GWGCWC 1 cut(s) 193
Alw26I GTCTC 2 cut(s) 118, 148
AlwI GGATC 1 cut(s) 387
AoxI GGCC 1 cut(s) 101
ApeKI GCWGC 2 cut(s) 112, 310
AspLEI GCGC 2 cut(s) 112, 117
AspS9I GGNCC 2 cut(s) 102, 135
AvaII GGWCC 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 490
Bbv12I GWGCWC 1 cut(s) 193
BbvI GCAGC 2 cut(s) 124, 322
BccI CCATC 3 cut(s) 85, 233, 473
BciT130I CCWGG 1 cut(s) 474
BcoDI GTCTC 2 cut(s) 118, 148
BcuI ACTAGT 1 cut(s) 386
BfaI CTAG 2 cut(s) 387, 494
BfrI CTTAAG 1 cut(s) 305
BisI GCNGC 2 cut(s) 113, 311
BlsI GCNGC 2 cut(s) 114, 312
Bme1390I CCNGG 1 cut(s) 474
Bme18I GGWCC 1 cut(s) 135
BmeRI GACNNNNNGTC 1 cut(s) 358
BmgT120I GGNCC 2 cut(s) 102, 135
BmrFI CCNGG 1 cut(s) 474
BmsI GCATC 1 cut(s) 392
BpiI GAAGAC 1 cut(s) 490
BpmI CTGGAG 1 cut(s) 495
BsaJI CCNNGG 1 cut(s) 472
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse1I ACTGG 1 cut(s) 130
BseBI CCWGG 1 cut(s) 474
BseDI CCNNGG 1 cut(s) 472
BseLI CCNNNNNNNGG 1 cut(s) 479
BseMII CTCAG 2 cut(s) 113, 274
BseNI ACTGG 1 cut(s) 130
BseXI GCAGC 2 cut(s) 124, 322
BseYI CCCAGC 1 cut(s) 16
Bsh1236I CGCG 1 cut(s) 110
BshFI GGCC 1 cut(s) 103
BsiHKAI GWGCWC 1 cut(s) 193
BsiSI CCGG 1 cut(s) 272
BslI CCNNNNNNNGG 1 cut(s) 479
BsmAI GTCTC 2 cut(s) 118, 148
BsnI GGCC 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 1 cut(s) 392
BspACI CCGC 2 cut(s) 108, 183
BspANI GGCC 1 cut(s) 103
BspCNI CTCAG 2 cut(s) 114, 273
BspFNI CGCG 1 cut(s) 110
BspPI GGATC 1 cut(s) 387
BspQI GCTCTTC 1 cut(s) 181
BspTI CTTAAG 1 cut(s) 305
BsrI ACTGG 1 cut(s) 130
BssECI CCNNGG 1 cut(s) 472
BssMI GATC 1 cut(s) 392
Bst2UI CCWGG 1 cut(s) 474
Bst6I CTCTTC 1 cut(s) 181
BstAFI CTTAAG 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 332
BstDEI CTNAG 2 cut(s) 122, 260
BstFNI CGCG 1 cut(s) 110
BstHHI GCGC 2 cut(s) 112, 117
BstKTI GATC 1 cut(s) 395
BstMAI GTCTC 2 cut(s) 118, 148
BstMBI GATC 1 cut(s) 392
BstMWI GCNNNNNNNGC 3 cut(s) 100, 109, 310
BstNI CCWGG 1 cut(s) 474
BstNSI RCATGY 2 cut(s) 238, 271
BstSCI CCNGG 1 cut(s) 472
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 2 cut(s) 124, 322
BstV2I GAAGAC 1 cut(s) 490
BstX2I RGATCY 1 cut(s) 392
BstYI RGATCY 1 cut(s) 392
BsuRI GGCC 1 cut(s) 103
BtsIMutI CAGTG 1 cut(s) 137
Cac8I GCNNGC 1 cut(s) 332
CfoI GCGC 2 cut(s) 112, 117
Cfr13I GGNCC 2 cut(s) 102, 135
Csp6I GTAC 1 cut(s) 528
CviAII CATG 4 cut(s) 235, 268, 355, 397
CviJI RGCY 8 cut(s) 8, 75, 94, 103, 146, 290, 334, 450
CviKI_1 RGCY 8 cut(s) 8, 75, 94, 103, 146, 290, 334, 450
CviQI GTAC 1 cut(s) 528
DdeI CTNAG 2 cut(s) 122, 260
DpnI GATC 1 cut(s) 394
DpnII GATC 1 cut(s) 392
DriI GACNNNNNGTC 1 cut(s) 358
Eam1104I CTCTTC 1 cut(s) 181
Eam1105I GACNNNNNGTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 135
EcoRII CCWGG 1 cut(s) 472
FaeI CATG 4 cut(s) 238, 271, 358, 400
FaiI YATR 8 cut(s) 236, 269, 278, 346, 356, 377, 398, 541
FatI CATG 4 cut(s) 234, 267, 354, 396
Fnu4HI GCNGC 2 cut(s) 113, 311
Fsp4HI GCNGC 2 cut(s) 113, 311
FspBI CTAG 2 cut(s) 387, 494
GlaI GCGC 2 cut(s) 111, 116
GluI GCNGC 2 cut(s) 113, 311
GsaI CCCAGC 1 cut(s) 20
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 1 cut(s) 103
HapII CCGG 1 cut(s) 272
HhaI GCGC 2 cut(s) 112, 117
Hin1II CATG 4 cut(s) 238, 271, 358, 400
Hin6I GCGC 2 cut(s) 110, 115
HinP1I GCGC 2 cut(s) 110, 115
HincII GTYRAC 1 cut(s) 420
HindII GTYRAC 1 cut(s) 420
HindIII AAGCTT 2 cut(s) 288, 332
HinfI GANTC 4 cut(s) 52, 61, 167, 490
HpaII CCGG 1 cut(s) 272
Hpy166II GTNNAC 3 cut(s) 211, 420, 431
Hpy188I TCNGA 4 cut(s) 36, 66, 327, 457
Hpy8I GTNNAC 3 cut(s) 211, 420, 431
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 1 cut(s) 249
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 109, 310
HpyF3I CTNAG 2 cut(s) 122, 260
Hsp92II CATG 4 cut(s) 238, 271, 358, 400
HspAI GCGC 2 cut(s) 110, 115
Kzo9I GATC 1 cut(s) 392
LguI GCTCTTC 1 cut(s) 181
LmnI GCTCC 1 cut(s) 327
Lsp1109I GCAGC 2 cut(s) 124, 322
LweI GCATC 1 cut(s) 392
MaeI CTAG 2 cut(s) 387, 494
MaeIII GTNAC 1 cut(s) 367
MalI GATC 1 cut(s) 394
MboI GATC 1 cut(s) 392
MboII GAAGA 3 cut(s) 168, 198, 490
MflI RGATCY 1 cut(s) 392
MhlI GDGCHC 1 cut(s) 193
MluCI AATT 2 cut(s) 299, 440
MlyI GAGTC 2 cut(s) 55, 176
MmeI TCCRAC 1 cut(s) 89
MnlI CCTC 1 cut(s) 93
MseI TTAA 1 cut(s) 306
MspA1I CMGCKG 1 cut(s) 183
MspCI CTTAAG 1 cut(s) 305
MspI CCGG 1 cut(s) 272
MspR9I CCNGG 1 cut(s) 474
MteI GCGCNGCGC 1 cut(s) 113
MvaI CCWGG 1 cut(s) 474
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 3 cut(s) 100, 109, 310
NdeII GATC 1 cut(s) 392
NlaIII CATG 4 cut(s) 238, 271, 358, 400
NmeAIII GCCGAG 1 cut(s) 70
NspI RCATGY 2 cut(s) 238, 271
PciSI GCTCTTC 1 cut(s) 181
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PfeI GAWTC 2 cut(s) 52, 490
PkrI GCNGC 2 cut(s) 114, 312
PleI GAGTC 2 cut(s) 55, 175
PpsI GAGTC 2 cut(s) 55, 175
Psp6I CCWGG 1 cut(s) 472
PspFI CCCAGC 1 cut(s) 16
PspGI CCWGG 1 cut(s) 472
PspPI GGNCC 2 cut(s) 102, 135
PsuI RGATCY 1 cut(s) 392
RsaI GTAC 1 cut(s) 529
RsaNI GTAC 1 cut(s) 528
SapI GCTCTTC 1 cut(s) 181
SaqAI TTAA 1 cut(s) 306
SatI GCNGC 2 cut(s) 113, 311
Sau3AI GATC 1 cut(s) 392
Sau96I GGNCC 2 cut(s) 102, 135
SchI GAGTC 2 cut(s) 55, 176
ScrFI CCNGG 1 cut(s) 474
SduI GDGCHC 1 cut(s) 193
SetI ASST 6 cut(s) 10, 123, 148, 222, 292, 336
SfaNI GCATC 1 cut(s) 392
SinI GGWCC 1 cut(s) 135
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
SpeI ACTAGT 1 cut(s) 386
Sse9I AATT 2 cut(s) 299, 440
SsiI CCGC 2 cut(s) 108, 183
SspMI CTAG 2 cut(s) 387, 494
StyD4I CCNGG 1 cut(s) 472
TaqI TCGA 1 cut(s) 41
TasI AATT 2 cut(s) 299, 440
TfiI GAWTC 2 cut(s) 52, 490
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TscAI CASTG 1 cut(s) 137
TseI GCWGC 2 cut(s) 112, 310
TspRI CASTG 1 cut(s) 137
Vha464I CTTAAG 1 cut(s) 305
VpaK11BI GGWCC 1 cut(s) 135
XceI RCATGY 2 cut(s) 238, 271
XspI CTAG 2 cut(s) 387, 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.