Rh1BG133500

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002855
Physical Location & Seq
Forward (+)
22319096 .. 22322016
2921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG133500.1

Sequence Viewer

Length: 753 bp
ATGTACTATGTAAAATTCAATGGAAAGAGCAAGGTTCACGATTTCGTTGGCTTCGTCTATAAATTCAGAATTTCTGGTCTTTTCCTGGTTATCAGAGAAATCTCCAAGCTTCATGAAGACATCGTCACACGCAGGCAGAAAAAACTTCTTATGAGACGTGACCGTCAGAAATATTTGGAAGAGGCAGCTTTACGAGAAGCTCAACTACTTCAAGAACTTGATAGGGAGAGAGCAGCTGAAGTTGAAAAGGATATCGAGAGACAGAGATTGGTAGAACTTGAGCGTGCAAAAACCAGGGAGCTGCGACAAAACCTTGAAATGGAGAAGGAAACACTAGCACAGAGAGATCTTCAGCGTGAATTGGAGCAAGCTGAAGCAGGTGTGCGATCGTCACGACGCGACTTTTCTTCTACTTACAGCAGTCGGCCCCGAGATCGGTACCGTGAAAGAGAAAATGGAAGAGCAGGTAATGAAGGTAGTACAAGATCAAGTAATACGCAGCTTGAAAGTTCATCCATCGGGACAATACCAACTGTGTCACGTGATCGTCTGGATGATGCTGGCAGTGGCTATGAAGAAAATTCTGATGGAAGCAAAGACTCTGGTGATACGGGTAGTGTTGGAGATCCCGACTCTTTATCAGCATTTGATGGACAGCCTGGTGGATTTGGCCCAGGCCAGAGGCATGGTTCTAGAGGGAGAAAATCCAGGCAGGTGGTGGAACGGAGGGAAAGGGATGTCAAAGTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

250

Amino Acids

28.75

Weight (kDa)

9.21

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 368, 703
AasI GACNNNNNNGTC 1 cut(s) 162
Acc36I ACCTGC 3 cut(s) 368, 455, 703
Acc65I GGTACC 1 cut(s) 438
AccB1I GGYRCC 1 cut(s) 438
AccII CGCG 1 cut(s) 399
AclWI GGATC 1 cut(s) 620
AcsI RAATTY 4 cut(s) 14, 62, 69, 580
AcuI CTGAAG 3 cut(s) 258, 335, 393
AcvI CACGTG 1 cut(s) 542
AfaI GTAC 3 cut(s) 5, 440, 481
AfiI CCNNNNNNNGG 2 cut(s) 319, 435
AgsI TTSAA 5 cut(s) 19, 212, 245, 317, 506
AjiI CACGTC 1 cut(s) 158
AjnI CCWGG 5 cut(s) 84, 293, 658, 673, 707
AluBI AGCT 7 cut(s) 109, 188, 200, 236, 301, 371, 502
AluI AGCT 7 cut(s) 109, 188, 200, 236, 301, 371, 502
Alw26I GTCTC 2 cut(s) 148, 253
AlwI GGATC 1 cut(s) 620
Ama87I CYCGRG 1 cut(s) 429
AoxI GGCC 3 cut(s) 425, 670, 676
ApeKI GCWGC 4 cut(s) 185, 233, 301, 499
ApoI RAATTY 4 cut(s) 14, 62, 69, 580
Asp718I GGTACC 1 cut(s) 438
AspS9I GGNCC 2 cut(s) 426, 671
AsuHPI GGTGA 1 cut(s) 617
AvaI CYCGRG 1 cut(s) 429
BanI GGYRCC 1 cut(s) 438
BarI GAAGNNNNNNTAC 2 cut(s) 189, 221
BbrPI CACGTG 1 cut(s) 542
BbsI GAAGAC 1 cut(s) 123
BbvI GCAGC 4 cut(s) 197, 245, 288, 511
BccI CCATC 3 cut(s) 524, 581, 644
BciT130I CCWGG 5 cut(s) 86, 295, 660, 675, 709
BcoDI GTCTC 2 cut(s) 148, 253
BfaI CTAG 2 cut(s) 335, 693
BfuAI ACCTGC 3 cut(s) 368, 455, 703
BglII AGATCT 1 cut(s) 346
BisI GCNGC 4 cut(s) 186, 234, 302, 500
BlsI GCNGC 4 cut(s) 187, 235, 303, 501
Bme1390I CCNGG 5 cut(s) 86, 295, 660, 675, 709
BmeT110I CYCGRG 1 cut(s) 429
BmgBI CACGTC 1 cut(s) 158
BmgT120I GGNCC 2 cut(s) 426, 671
BmiI GGNNCC 2 cut(s) 428, 440
BmrFI CCNGG 5 cut(s) 86, 295, 660, 675, 709
BmsI GCATC 1 cut(s) 547
BpiI GAAGAC 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 299
BsaAI YACGTR 1 cut(s) 542
BsaJI CCNNGG 2 cut(s) 294, 673
Bsc4I CCNNNNNNNGG 2 cut(s) 319, 435
BseBI CCWGG 5 cut(s) 86, 295, 660, 675, 709
BseDI CCNNGG 2 cut(s) 294, 673
BseGI GGATG 3 cut(s) 512, 559, 742
BseLI CCNNNNNNNGG 2 cut(s) 319, 435
BseXI GCAGC 4 cut(s) 197, 245, 288, 511
Bsh1236I CGCG 1 cut(s) 399
Bsh1285I CGRYCG 1 cut(s) 389
BshFI GGCC 3 cut(s) 427, 672, 678
BshNI GGYRCC 1 cut(s) 438
BsiEI CGRYCG 1 cut(s) 389
BsiHKCI CYCGRG 1 cut(s) 429
BslFI GGGAC 1 cut(s) 535
BslI CCNNNNNNNGG 2 cut(s) 319, 435
BsmAI GTCTC 2 cut(s) 148, 253
BsmBI CGTCTC 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 535
BsnI GGCC 3 cut(s) 427, 672, 678
BsoBI CYCGRG 1 cut(s) 429
Bsp143I GATC 6 cut(s) 346, 386, 433, 485, 544, 625
BspANI GGCC 3 cut(s) 427, 672, 678
BspFNI CGCG 1 cut(s) 399
BspHI TCATGA 1 cut(s) 112
BspLI GGNNCC 2 cut(s) 428, 440
BspMI ACCTGC 3 cut(s) 368, 455, 703
BspPI GGATC 1 cut(s) 620
BspQI GCTCTTC 1 cut(s) 454
BspT107I GGYRCC 1 cut(s) 438
BssECI CCNNGG 2 cut(s) 294, 673
BssMI GATC 6 cut(s) 346, 386, 433, 485, 544, 625
Bst2UI CCWGG 5 cut(s) 86, 295, 660, 675, 709
Bst4CI ACNGT 3 cut(s) 164, 443, 535
Bst6I CTCTTC 2 cut(s) 174, 454
BstBAI YACGTR 1 cut(s) 542
BstC8I GCNNGC 4 cut(s) 134, 285, 369, 562
BstF5I GGATG 3 cut(s) 512, 559, 742
BstFNI CGCG 1 cut(s) 399
BstKTI GATC 6 cut(s) 349, 389, 436, 488, 547, 628
BstMAI GTCTC 2 cut(s) 148, 253
BstMBI GATC 6 cut(s) 346, 386, 433, 485, 544, 625
BstMCI CGRYCG 1 cut(s) 389
BstNI CCWGG 5 cut(s) 86, 295, 660, 675, 709
BstSCI CCNGG 5 cut(s) 84, 293, 658, 673, 707
BstUI CGCG 1 cut(s) 399
BstV1I GCAGC 4 cut(s) 197, 245, 288, 511
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 2 cut(s) 346, 625
BstXI CCANNNNNNTGG 2 cut(s) 686, 715
BstYI RGATCY 2 cut(s) 346, 625
BsuRI GGCC 3 cut(s) 427, 672, 678
BtrI CACGTC 1 cut(s) 158
BtsCI GGATG 3 cut(s) 512, 559, 742
BtsI GCAGTG 1 cut(s) 571
BtsIMutI CAGTG 1 cut(s) 571
BveI ACCTGC 3 cut(s) 368, 455, 703
Cac8I GCNNGC 4 cut(s) 134, 285, 369, 562
CciI TCATGA 1 cut(s) 112
Cfr13I GGNCC 2 cut(s) 426, 671
CseI GACGC 1 cut(s) 405
Csp6I GTAC 3 cut(s) 4, 439, 480
CviAII CATG 2 cut(s) 113, 686
CviQI GTAC 3 cut(s) 4, 439, 480
DpnI GATC 6 cut(s) 348, 388, 435, 487, 546, 627
DpnII GATC 6 cut(s) 346, 386, 433, 485, 544, 625
DrdI GACNNNNNNGTC 1 cut(s) 162
DseDI GACNNNNNNGTC 1 cut(s) 162
Eam1104I CTCTTC 2 cut(s) 174, 454
EarI CTCTTC 2 cut(s) 174, 454
Eco32I GATATC 1 cut(s) 253
Eco57I CTGAAG 3 cut(s) 258, 335, 393
Eco72I CACGTG 1 cut(s) 542
Eco88I CYCGRG 1 cut(s) 429
EcoRII CCWGG 5 cut(s) 84, 293, 658, 673, 707
EcoRV GATATC 1 cut(s) 253
Esp3I CGTCTC 1 cut(s) 148
FaeI CATG 2 cut(s) 116, 689
FaiI YATR 6 cut(s) 9, 60, 114, 152, 573, 687
FaqI GGGAC 1 cut(s) 535
FatI CATG 2 cut(s) 112, 685
Fnu4HI GCNGC 4 cut(s) 186, 234, 302, 500
FokI GGATG 3 cut(s) 499, 566, 749
Fsp4HI GCNGC 4 cut(s) 186, 234, 302, 500
FspBI CTAG 2 cut(s) 335, 693
GluI GCNGC 4 cut(s) 186, 234, 302, 500
HaeIII GGCC 3 cut(s) 427, 672, 678
HgaI GACGC 1 cut(s) 405
Hin1II CATG 2 cut(s) 116, 689
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 2 cut(s) 599, 632
HphI GGTGA 1 cut(s) 617
Hpy166II GTNNAC 2 cut(s) 37, 748
Hpy188I TCNGA 4 cut(s) 68, 95, 168, 586
Hpy188III TCNNGA 9 cut(s) 38, 113, 212, 256, 393, 520, 551, 629, 693
Hpy8I GTNNAC 2 cut(s) 37, 748
Hpy99I CGWCG 1 cut(s) 399
HpyAV CCTTC 2 cut(s) 319, 467
HpyCH4III ACNGT 3 cut(s) 164, 443, 535
HpyCH4IV ACGT 2 cut(s) 157, 541
HpyCH4V TGCA 1 cut(s) 287
HpySE526I ACGT 2 cut(s) 157, 541
Hsp92II CATG 2 cut(s) 116, 689
KpnI GGTACC 1 cut(s) 442
Kzo9I GATC 6 cut(s) 346, 386, 433, 485, 544, 625
LguI GCTCTTC 1 cut(s) 454
LmnI GCTCC 2 cut(s) 298, 364
Lsp1109I GCAGC 4 cut(s) 197, 245, 288, 511
LweI GCATC 1 cut(s) 547
MaeI CTAG 2 cut(s) 335, 693
MaeII ACGT 2 cut(s) 157, 541
MaeIII GTNAC 4 cut(s) 124, 158, 390, 537
MalI GATC 6 cut(s) 348, 388, 435, 487, 546, 627
MboI GATC 6 cut(s) 346, 386, 433, 485, 544, 625
MboII GAAGA 6 cut(s) 128, 191, 341, 399, 471, 587
MflI RGATCY 2 cut(s) 346, 625
MluCI AATT 5 cut(s) 14, 62, 69, 359, 580
MlyI GAGTC 2 cut(s) 593, 626
MmeI TCCRAC 1 cut(s) 601
MnlI CCTC 4 cut(s) 175, 675, 689, 720
MspA1I CMGCKG 1 cut(s) 236
MspR9I CCNGG 5 cut(s) 86, 295, 660, 675, 709
MvaI CCWGG 5 cut(s) 86, 295, 660, 675, 709
MvnI CGCG 1 cut(s) 399
NdeII GATC 6 cut(s) 346, 386, 433, 485, 544, 625
NlaIII CATG 2 cut(s) 116, 689
NlaIV GGNNCC 2 cut(s) 428, 440
NmuCI GTSAC 4 cut(s) 124, 158, 390, 537
PagI TCATGA 1 cut(s) 112
PaqCI CACCTGC 2 cut(s) 368, 703
PciSI GCTCTTC 1 cut(s) 454
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PflFI GACNNNGTC 1 cut(s) 122
PkrI GCNGC 4 cut(s) 187, 235, 303, 501
Ple19I CGATCG 1 cut(s) 389
PleI GAGTC 2 cut(s) 593, 626
PmaCI CACGTG 1 cut(s) 542
PmlI CACGTG 1 cut(s) 542
PpsI GAGTC 2 cut(s) 593, 626
Ppu21I YACGTR 1 cut(s) 542
Psp6I CCWGG 5 cut(s) 84, 293, 658, 673, 707
PspCI CACGTG 1 cut(s) 542
PspGI CCWGG 5 cut(s) 84, 293, 658, 673, 707
PspN4I GGNNCC 2 cut(s) 428, 440
PspPI GGNCC 2 cut(s) 426, 671
PsuI RGATCY 2 cut(s) 346, 625
PsyI GACNNNGTC 1 cut(s) 122
PvuI CGATCG 1 cut(s) 389
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 3 cut(s) 5, 440, 481
RsaNI GTAC 3 cut(s) 4, 439, 480
SapI GCTCTTC 1 cut(s) 454
SatI GCNGC 4 cut(s) 186, 234, 302, 500
Sau3AI GATC 6 cut(s) 346, 386, 433, 485, 544, 625
Sau96I GGNCC 2 cut(s) 426, 671
SchI GAGTC 2 cut(s) 593, 626
ScrFI CCNGG 5 cut(s) 86, 295, 660, 675, 709
SfaNI GCATC 1 cut(s) 547
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
Sse9I AATT 5 cut(s) 14, 62, 69, 359, 580
SspI AATATT 1 cut(s) 173
SspMI CTAG 2 cut(s) 335, 693
StyD4I CCNGG 5 cut(s) 84, 293, 658, 673, 707
TaaI ACNGT 3 cut(s) 164, 443, 535
TaiI ACGT 2 cut(s) 160, 544
TaqI TCGA 1 cut(s) 255
TasI AATT 5 cut(s) 14, 62, 69, 359, 580
TatI WGTACW 2 cut(s) 3, 479
TscAI CASTG 1 cut(s) 571
TseFI GTSAC 4 cut(s) 124, 158, 390, 537
TseI GCWGC 4 cut(s) 185, 233, 301, 499
Tsp45I GTSAC 4 cut(s) 124, 158, 390, 537
TspDTI ATGAA 5 cut(s) 101, 129, 486, 501, 588
TspGWI ACGGA 1 cut(s) 739
TspRI CASTG 1 cut(s) 571
Tth111I GACNNNGTC 1 cut(s) 122
XapI RAATTY 4 cut(s) 14, 62, 69, 580
XbaI TCTAGA 1 cut(s) 692
XcmI CCANNNNNNNNNTGG 1 cut(s) 715
XspI CTAG 2 cut(s) 335, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.