Rh1DG168100

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0002855
Physical Location & Seq
Forward (+)
34472322 .. 34488930
16609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG168100.1

Sequence Viewer

Length: 678 bp
ATGGACGACGAACGCCTCAATGAGGAAGTGGAGGAGGCTGAATTAGAGTTGGACATTCTTCAGAAAGAAGATGCATTGCAAGAATCACCTAAAGATACCAAAGATAAACACCAGATTCCTTTTCTAAGCTCTCCTCTGAAAGATAATGCGCGCCTCCAGCAAATCAAGGATTGCATTCATTCCTTAGAAATCTCCAAGCTTCATGAAGACATCGTCACACGCAGGCAGAAAAAACTTCTTATGAGACGTGACCGTCAGAAATATTTGGAAGAGGCAGCTTTACGAGAAGCTCAACTACTTCAAGAACTTGATAGAGAGATCTTCAGCGTGAATTGGAGCAAGCTGAAGCAGGTGTGCGATCGTCACGACGCGACTTTTCTTCTACTTACAGCAGTCGGCCCCGAGATCGGTACCGTGAAAGAGAAAATGGAAGAGCAGTCACGTGATCGTCTGGATGATGCTGGCAGTGGCTATGAAGAAAATTCTGATGGAAGCAAAGACTCTGGTGATACGGGTAGTGTTGGAGATCCCGACTCTTTATCAGCATTTGATGGACAGCCTGGTGGATTTGGCCCAGGCCAGAGGCATGGTTCTAGAGGGAGAAAATCCAGGCAGGTGGTGGAACGGAGGGAAAGGGATGGTAAACGAGAAGGAAAGTGGGAAAGAAAACATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

225

Amino Acids

25.81

Weight (kDa)

5.34

Isoelectric Point (pI)

53.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 340, 604
AasI GACNNNNNNGTC 1 cut(s) 252
Acc36I ACCTGC 2 cut(s) 340, 604
Acc65I GGTACC 1 cut(s) 410
AccB1I GGYRCC 1 cut(s) 410
AccII CGCG 2 cut(s) 151, 371
AclWI GGATC 1 cut(s) 521
AcsI RAATTY 1 cut(s) 481
AcuI CTGAAG 3 cut(s) 44, 307, 365
AcvI CACGTG 1 cut(s) 443
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 2 cut(s) 22, 407
AgsI TTSAA 1 cut(s) 302
AjiI CACGTC 1 cut(s) 248
AjnI CCWGG 3 cut(s) 559, 574, 608
AluBI AGCT 5 cut(s) 129, 199, 278, 290, 343
AluI AGCT 5 cut(s) 129, 199, 278, 290, 343
Alw26I GTCTC 1 cut(s) 238
AlwI GGATC 1 cut(s) 521
Ama87I CYCGRG 1 cut(s) 401
AoxI GGCC 3 cut(s) 397, 571, 577
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 1 cut(s) 481
Asp718I GGTACC 1 cut(s) 410
AspLEI GCGC 2 cut(s) 151, 153
AspS9I GGNCC 2 cut(s) 398, 572
AsuHPI GGTGA 2 cut(s) 78, 518
AvaI CYCGRG 1 cut(s) 401
BanI GGYRCC 1 cut(s) 410
BarI GAAGNNNNNNTAC 2 cut(s) 279, 311
BbrPI CACGTG 1 cut(s) 443
BbsI GAAGAC 1 cut(s) 213
BbvI GCAGC 1 cut(s) 287
BccI CCATC 3 cut(s) 482, 545, 632
BciT130I CCWGG 3 cut(s) 561, 576, 610
BcoDI GTCTC 1 cut(s) 238
BfaI CTAG 1 cut(s) 594
BfuAI ACCTGC 2 cut(s) 340, 604
BglII AGATCT 1 cut(s) 318
BisI GCNGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 277
Bme1390I CCNGG 3 cut(s) 561, 576, 610
BmeT110I CYCGRG 1 cut(s) 401
BmgBI CACGTC 1 cut(s) 248
BmgT120I GGNCC 2 cut(s) 398, 572
BmiI GGNNCC 2 cut(s) 400, 412
BmrFI CCNGG 3 cut(s) 561, 576, 610
BmsI GCATC 2 cut(s) 61, 448
BpiI GAAGAC 1 cut(s) 213
BpmI CTGGAG 1 cut(s) 140
BsaAI YACGTR 1 cut(s) 443
BsaJI CCNNGG 1 cut(s) 574
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 407
Bse3DI GCAATG 1 cut(s) 74
BseBI CCWGG 3 cut(s) 561, 576, 610
BseDI CCNNGG 1 cut(s) 574
BseGI GGATG 2 cut(s) 460, 643
BseLI CCNNNNNNNGG 2 cut(s) 22, 407
BseMI GCAATG 1 cut(s) 74
BsePI GCGCGC 1 cut(s) 149
BseRI GAGGAG 2 cut(s) 47, 123
BseXI GCAGC 1 cut(s) 287
Bsh1236I CGCG 2 cut(s) 151, 371
Bsh1285I CGRYCG 1 cut(s) 361
BshFI GGCC 3 cut(s) 399, 573, 579
BshNI GGYRCC 1 cut(s) 410
BsiEI CGRYCG 1 cut(s) 361
BsiHKCI CYCGRG 1 cut(s) 401
BslI CCNNNNNNNGG 2 cut(s) 22, 407
BsmAI GTCTC 1 cut(s) 238
BsmBI CGTCTC 1 cut(s) 238
BsmI GAATGC 1 cut(s) 174
BsnI GGCC 3 cut(s) 399, 573, 579
BsoBI CYCGRG 1 cut(s) 401
Bsp143I GATC 5 cut(s) 318, 358, 405, 445, 526
BspANI GGCC 3 cut(s) 399, 573, 579
BspFNI CGCG 2 cut(s) 151, 371
BspHI TCATGA 1 cut(s) 202
BspLI GGNNCC 2 cut(s) 400, 412
BspMI ACCTGC 2 cut(s) 340, 604
BspPI GGATC 1 cut(s) 521
BspQI GCTCTTC 1 cut(s) 426
BspT107I GGYRCC 1 cut(s) 410
BsrDI GCAATG 1 cut(s) 74
BssECI CCNNGG 1 cut(s) 574
BssHII GCGCGC 1 cut(s) 149
BssMI GATC 5 cut(s) 318, 358, 405, 445, 526
Bst2UI CCWGG 3 cut(s) 561, 576, 610
Bst4CI ACNGT 2 cut(s) 254, 415
Bst6I CTCTTC 2 cut(s) 264, 426
BstBAI YACGTR 1 cut(s) 443
BstC8I GCNNGC 4 cut(s) 151, 224, 341, 463
BstDEI CTNAG 2 cut(s) 125, 184
BstENI CCTNNNNNAGG 1 cut(s) 20
BstF5I GGATG 2 cut(s) 460, 643
BstFNI CGCG 2 cut(s) 151, 371
BstHHI GCGC 2 cut(s) 151, 153
BstKTI GATC 5 cut(s) 321, 361, 408, 448, 529
BstMAI GTCTC 1 cut(s) 238
BstMBI GATC 5 cut(s) 318, 358, 405, 445, 526
BstMCI CGRYCG 1 cut(s) 361
BstMWI GCNNNNNNNGC 1 cut(s) 157
BstNI CCWGG 3 cut(s) 561, 576, 610
BstSCI CCNGG 3 cut(s) 559, 574, 608
BstUI CGCG 2 cut(s) 151, 371
BstV1I GCAGC 1 cut(s) 287
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 2 cut(s) 318, 526
BstXI CCANNNNNNTGG 2 cut(s) 587, 616
BstYI RGATCY 2 cut(s) 318, 526
BsuRI GGCC 3 cut(s) 399, 573, 579
BtrI CACGTC 1 cut(s) 248
BtsCI GGATG 2 cut(s) 460, 643
BtsI GCAGTG 1 cut(s) 472
BtsIMutI CAGTG 1 cut(s) 472
BveI ACCTGC 2 cut(s) 340, 604
Cac8I GCNNGC 4 cut(s) 151, 224, 341, 463
CciI TCATGA 1 cut(s) 202
CfoI GCGC 2 cut(s) 151, 153
Cfr13I GGNCC 2 cut(s) 398, 572
CseI GACGC 1 cut(s) 377
Csp6I GTAC 1 cut(s) 411
CviAII CATG 2 cut(s) 203, 587
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 2 cut(s) 125, 184
DpnI GATC 5 cut(s) 320, 360, 407, 447, 528
DpnII GATC 5 cut(s) 318, 358, 405, 445, 526
DrdI GACNNNNNNGTC 1 cut(s) 252
DseDI GACNNNNNNGTC 1 cut(s) 252
Eam1104I CTCTTC 2 cut(s) 264, 426
EarI CTCTTC 2 cut(s) 264, 426
Eco57I CTGAAG 3 cut(s) 44, 307, 365
Eco72I CACGTG 1 cut(s) 443
Eco88I CYCGRG 1 cut(s) 401
EcoNI CCTNNNNNAGG 1 cut(s) 20
EcoRII CCWGG 3 cut(s) 559, 574, 608
EcoT22I ATGCAT 1 cut(s) 76
Esp3I CGTCTC 1 cut(s) 238
FaeI CATG 2 cut(s) 206, 590
FaiI YATR 5 cut(s) 204, 242, 474, 588, 676
FatI CATG 2 cut(s) 202, 586
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 2 cut(s) 467, 650
Fsp4HI GCNGC 1 cut(s) 276
FspBI CTAG 1 cut(s) 594
GlaI GCGC 2 cut(s) 150, 152
GluI GCNGC 1 cut(s) 276
GsuI CTGGAG 1 cut(s) 140
HaeIII GGCC 3 cut(s) 399, 573, 579
HgaI GACGC 1 cut(s) 377
HhaI GCGC 2 cut(s) 151, 153
Hin1II CATG 2 cut(s) 206, 590
Hin6I GCGC 2 cut(s) 149, 151
HinP1I GCGC 2 cut(s) 149, 151
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 4 cut(s) 83, 115, 500, 533
HphI GGTGA 2 cut(s) 78, 518
Hpy166II GTNNAC 1 cut(s) 644
Hpy188I TCNGA 4 cut(s) 63, 138, 258, 487
Hpy188III TCNNGA 6 cut(s) 203, 302, 365, 452, 530, 594
Hpy8I GTNNAC 1 cut(s) 644
Hpy99I CGWCG 2 cut(s) 11, 371
HpyAV CCTTC 1 cut(s) 644
HpyCH4III ACNGT 2 cut(s) 254, 415
HpyCH4IV ACGT 2 cut(s) 247, 442
HpyCH4V TGCA 3 cut(s) 74, 79, 174
HpyF10VI GCNNNNNNNGC 1 cut(s) 157
HpyF3I CTNAG 2 cut(s) 125, 184
HpySE526I ACGT 2 cut(s) 247, 442
Hsp92II CATG 2 cut(s) 206, 590
HspAI GCGC 2 cut(s) 149, 151
KpnI GGTACC 1 cut(s) 414
Kzo9I GATC 5 cut(s) 318, 358, 405, 445, 526
LguI GCTCTTC 1 cut(s) 426
LmnI GCTCC 1 cut(s) 336
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 2 cut(s) 61, 448
MaeI CTAG 1 cut(s) 594
MaeII ACGT 2 cut(s) 247, 442
MaeIII GTNAC 4 cut(s) 214, 248, 362, 438
MalI GATC 5 cut(s) 320, 360, 407, 447, 528
MboI GATC 5 cut(s) 318, 358, 405, 445, 526
MboII GAAGA 8 cut(s) 50, 80, 218, 281, 313, 371, 443, 488
MflI RGATCY 2 cut(s) 318, 526
MluCI AATT 3 cut(s) 41, 331, 481
MlyI GAGTC 2 cut(s) 494, 527
MmeI TCCRAC 2 cut(s) 30, 502
Mph1103I ATGCAT 1 cut(s) 76
MspR9I CCNGG 3 cut(s) 561, 576, 610
Mva1269I GAATGC 1 cut(s) 174
MvaI CCWGG 3 cut(s) 561, 576, 610
MvnI CGCG 2 cut(s) 151, 371
MwoI GCNNNNNNNGC 1 cut(s) 157
NdeII GATC 5 cut(s) 318, 358, 405, 445, 526
NlaIII CATG 2 cut(s) 206, 590
NlaIV GGNNCC 2 cut(s) 400, 412
NmuCI GTSAC 4 cut(s) 214, 248, 362, 438
NsiI ATGCAT 1 cut(s) 76
PagI TCATGA 1 cut(s) 202
PaqCI CACCTGC 2 cut(s) 340, 604
PauI GCGCGC 1 cut(s) 149
PciSI GCTCTTC 1 cut(s) 426
PctI GAATGC 1 cut(s) 174
PfeI GAWTC 2 cut(s) 83, 115
PflFI GACNNNGTC 1 cut(s) 212
PkrI GCNGC 1 cut(s) 277
Ple19I CGATCG 1 cut(s) 361
PleI GAGTC 2 cut(s) 494, 527
PmaCI CACGTG 1 cut(s) 443
PmlI CACGTG 1 cut(s) 443
PpsI GAGTC 2 cut(s) 494, 527
Ppu21I YACGTR 1 cut(s) 443
Psp6I CCWGG 3 cut(s) 559, 574, 608
PspCI CACGTG 1 cut(s) 443
PspGI CCWGG 3 cut(s) 559, 574, 608
PspN4I GGNNCC 2 cut(s) 400, 412
PspPI GGNCC 2 cut(s) 398, 572
PsuI RGATCY 2 cut(s) 318, 526
PsyI GACNNNGTC 1 cut(s) 212
PteI GCGCGC 1 cut(s) 149
PvuI CGATCG 1 cut(s) 361
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
SapI GCTCTTC 1 cut(s) 426
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 5 cut(s) 318, 358, 405, 445, 526
Sau96I GGNCC 2 cut(s) 398, 572
SchI GAGTC 2 cut(s) 494, 527
ScrFI CCNGG 3 cut(s) 561, 576, 610
SfaNI GCATC 2 cut(s) 61, 448
Sse9I AATT 3 cut(s) 41, 331, 481
SspI AATATT 1 cut(s) 263
SspMI CTAG 1 cut(s) 594
StyD4I CCNGG 3 cut(s) 559, 574, 608
TaaI ACNGT 2 cut(s) 254, 415
TaiI ACGT 2 cut(s) 250, 445
TasI AATT 3 cut(s) 41, 331, 481
TfiI GAWTC 2 cut(s) 83, 115
TscAI CASTG 1 cut(s) 472
TseFI GTSAC 4 cut(s) 214, 248, 362, 438
TseI GCWGC 1 cut(s) 275
Tsp45I GTSAC 4 cut(s) 214, 248, 362, 438
TspDTI ATGAA 4 cut(s) 167, 191, 219, 489
TspGWI ACGGA 1 cut(s) 640
TspRI CASTG 1 cut(s) 472
Tth111I GACNNNGTC 1 cut(s) 212
XagI CCTNNNNNAGG 1 cut(s) 20
XapI RAATTY 1 cut(s) 481
XbaI TCTAGA 1 cut(s) 593
XcmI CCANNNNNNNNNTGG 1 cut(s) 616
XspI CTAG 1 cut(s) 594
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.