Rh2CG557700

Inner membrane component of T3SS, cytoplasmic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003377
Physical Location & Seq
Reverse (-)
73599633 .. 73600826
1194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG557700.1

Sequence Viewer

Length: 366 bp
ATGAAAAAAGAACAGAACAGATCAGCATCAGAAACTACAGAGTACACTGATGCTGGAGAAGCACTGGATGAATACATGTCTGGGGTTTCATCTAAGCTAGTGCTTGATAAAACTGAGCAATTGCAAAAGGAACTCTCAGATCTCCAGTTCGAATATGACAGGGCAGTCTTCCTTTTGAAGATTGCTGATTCAACAGGTGAAGCAGCCAAGAAAAGGGATTCAAAGGTGCTTCCTGAAAATCCTGAAACTTCTGCTGCTGCCATCAAGAAGCAACATCCATTTAAACCAAAGGAAACCTGTCTGCCTGAAAATCCAGAAAGTGGCTATAAAGAAAGAGGAGAGTACAGATGTGACTGTTGCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FHA

Protein Analysis

121

Amino Acids

13.66

Weight (kDa)

5.03

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AccB7I CCANNNNNTGG 1 cut(s) 320
AfaI GTAC 2 cut(s) 44, 344
AfiI CCNNNNNNNGG 2 cut(s) 213, 320
AflIII ACRYGT 1 cut(s) 75
AgsI TTSAA 3 cut(s) 178, 192, 222
AluBI AGCT 1 cut(s) 97
AluI AGCT 1 cut(s) 97
ApeKI GCWGC 3 cut(s) 203, 254, 257
AsuHPI GGTGA 1 cut(s) 209
AsuII TTCGAA 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 160
BbvI GCAGC 3 cut(s) 215, 241, 244
BccI CCATC 1 cut(s) 269
BfaI CTAG 2 cut(s) 98, 364
BfmI CTRYAG 1 cut(s) 36
BglII AGATCT 1 cut(s) 139
BisI GCNGC 3 cut(s) 204, 255, 258
BlsI GCNGC 3 cut(s) 205, 256, 259
BmsI GCATC 2 cut(s) 35, 40
BpiI GAAGAC 1 cut(s) 160
BpmI CTGGAG 2 cut(s) 75, 128
Bpu14I TTCGAA 1 cut(s) 150
BsaBI GATNNNNATC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 2 cut(s) 213, 320
Bse1I ACTGG 2 cut(s) 69, 145
Bse8I GATNNNNATC 1 cut(s) 25
BseGI GGATG 2 cut(s) 73, 274
BseJI GATNNNNATC 1 cut(s) 25
BseLI CCNNNNNNNGG 2 cut(s) 213, 320
BseMII CTCAG 2 cut(s) 105, 150
BseNI ACTGG 2 cut(s) 69, 145
BseRI GAGGAG 1 cut(s) 351
BseXI GCAGC 3 cut(s) 215, 241, 244
BslI CCNNNNNNNGG 2 cut(s) 213, 320
Bsp119I TTCGAA 1 cut(s) 150
Bsp143I GATC 2 cut(s) 20, 139
BspCNI CTCAG 2 cut(s) 106, 149
BspT104I TTCGAA 1 cut(s) 150
BsrI ACTGG 2 cut(s) 69, 145
BssMI GATC 2 cut(s) 20, 139
Bst4CI ACNGT 1 cut(s) 356
BstBI TTCGAA 1 cut(s) 150
BstDEI CTNAG 3 cut(s) 93, 114, 136
BstF5I GGATG 2 cut(s) 73, 274
BstKTI GATC 2 cut(s) 23, 142
BstMBI GATC 2 cut(s) 20, 139
BstMWI GCNNNNNNNGC 1 cut(s) 59
BstNSI RCATGY 1 cut(s) 79
BstSFI CTRYAG 1 cut(s) 36
BstV1I GCAGC 3 cut(s) 215, 241, 244
BstV2I GAAGAC 1 cut(s) 160
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
BtsCI GGATG 2 cut(s) 73, 274
BtsIMutI CAGTG 2 cut(s) 45, 62
Csp6I GTAC 2 cut(s) 43, 343
CviAII CATG 1 cut(s) 76
CviJI RGCY 3 cut(s) 97, 206, 324
CviKI_1 RGCY 3 cut(s) 97, 206, 324
CviQI GTAC 2 cut(s) 43, 343
DdeI CTNAG 3 cut(s) 93, 114, 136
DpnI GATC 2 cut(s) 22, 141
DpnII GATC 2 cut(s) 20, 139
DraI TTTAAA 1 cut(s) 283
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
FaeI CATG 1 cut(s) 79
FaiI YATR 3 cut(s) 77, 156, 327
FatI CATG 1 cut(s) 75
Fnu4HI GCNGC 3 cut(s) 204, 255, 258
FokI GGATG 2 cut(s) 80, 261
Fsp4HI GCNGC 3 cut(s) 204, 255, 258
FspBI CTAG 2 cut(s) 98, 364
GluI GCNGC 3 cut(s) 204, 255, 258
GsuI CTGGAG 2 cut(s) 75, 128
Hin1II CATG 1 cut(s) 79
HinfI GANTC 2 cut(s) 188, 218
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 1 cut(s) 45
Hpy188I TCNGA 2 cut(s) 31, 139
Hpy188III TCNNGA 4 cut(s) 233, 242, 265, 314
Hpy8I GTNNAC 1 cut(s) 45
HpyCH4III ACNGT 1 cut(s) 356
HpyCH4V TGCA 2 cut(s) 124, 360
HpyF10VI GCNNNNNNNGC 1 cut(s) 59
HpyF3I CTNAG 3 cut(s) 93, 114, 136
Hsp92II CATG 1 cut(s) 79
Kzo9I GATC 2 cut(s) 20, 139
Lsp1109I GCAGC 3 cut(s) 215, 241, 244
LweI GCATC 2 cut(s) 35, 40
MaeI CTAG 2 cut(s) 98, 364
MaeIII GTNAC 1 cut(s) 350
MalI GATC 2 cut(s) 22, 141
MboI GATC 2 cut(s) 20, 139
MboII GAAGA 2 cut(s) 160, 190
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 1 cut(s) 139
MluCI AATT 1 cut(s) 119
MnlI CCTC 1 cut(s) 329
MseI TTAA 1 cut(s) 282
MunI CAATTG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 59
NdeII GATC 2 cut(s) 20, 139
NlaIII CATG 1 cut(s) 79
NmuCI GTSAC 1 cut(s) 350
NspI RCATGY 1 cut(s) 79
NspV TTCGAA 1 cut(s) 150
PciI ACATGT 1 cut(s) 75
PfeI GAWTC 2 cut(s) 188, 218
PflMI CCANNNNNTGG 1 cut(s) 320
PkrI GCNGC 3 cut(s) 205, 256, 259
PscI ACATGT 1 cut(s) 75
PsuI RGATCY 1 cut(s) 139
RsaI GTAC 2 cut(s) 44, 344
RsaNI GTAC 2 cut(s) 43, 343
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 3 cut(s) 204, 255, 258
Sau3AI GATC 2 cut(s) 20, 139
SetI ASST 4 cut(s) 99, 199, 228, 299
SfaNI GCATC 2 cut(s) 35, 40
SfcI CTRYAG 1 cut(s) 36
SfuI TTCGAA 1 cut(s) 150
Sse9I AATT 1 cut(s) 119
SspMI CTAG 2 cut(s) 98, 364
TaaI ACNGT 1 cut(s) 356
TaqI TCGA 1 cut(s) 150
TasI AATT 1 cut(s) 119
TatI WGTACW 2 cut(s) 42, 342
TfiI GAWTC 2 cut(s) 188, 218
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 2 cut(s) 52, 69
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 3 cut(s) 203, 254, 257
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 3 cut(s) 17, 78, 84
TspRI CASTG 2 cut(s) 52, 69
Van91I CCANNNNNTGG 1 cut(s) 320
XceI RCATGY 1 cut(s) 79
XspI CTAG 2 cut(s) 98, 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.