Rh2DG596400

Inner membrane component of T3SS, cytoplasmic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003377
Physical Location & Seq
Reverse (-)
82928391 .. 82928696
306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG596400.1

Sequence Viewer

Length: 306 bp
ATGTGTGTTGCATCTAGCAAGAAGCCGGAGTTAGGTGAGGTTCTCACTGATGCAACTGAAGGAAAAAGTGTTGTGTATACTCTTGCAAAGCCCCAGTGGCTTGGAGCTATAGTGGACAAGAAGATCGTGGAGGGTCATCAAGAGGCAGCATCTACGAATGAGCATGAGTCTGAAGTATTTGTGGACTATACAGACAGGAAGAAGATTTTGGAGAATGAAGTTAACATGGAATCTGGCTGCGCCTGGTTTGATTATAAGGAACGAAAACAAGTTCATGAATCTGAAGCTAGTGATGATTCTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FHA

Protein Analysis

101

Amino Acids

11.36

Weight (kDa)

4.82

Isoelectric Point (pI)

19.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 255
AccI GTMKAC 1 cut(s) 77
AcuI CTGAAG 3 cut(s) 78, 192, 303
AfiI CCNNNNNNNGG 1 cut(s) 32
AjnI CCWGG 1 cut(s) 242
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AluBI AGCT 2 cut(s) 107, 287
AluI AGCT 2 cut(s) 107, 287
ApeKI GCWGC 2 cut(s) 146, 237
AspLEI GCGC 1 cut(s) 242
AsuHPI GGTGA 1 cut(s) 47
BbvI GCAGC 2 cut(s) 158, 224
BciT130I CCWGG 1 cut(s) 244
BfaI CTAG 2 cut(s) 15, 288
BfmI CTRYAG 1 cut(s) 108
BglI GCCNNNNNGGC 1 cut(s) 97
BisI GCNGC 2 cut(s) 147, 238
BlsI GCNGC 2 cut(s) 148, 239
Bme1390I CCNGG 1 cut(s) 244
BmrFI CCNGG 1 cut(s) 244
BmrI ACTGGG 1 cut(s) 88
BmsI GCATC 3 cut(s) 20, 40, 158
BmuI ACTGGG 1 cut(s) 88
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 94
BseBI CCWGG 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 32
BseNI ACTGG 1 cut(s) 94
BseXI GCAGC 2 cut(s) 158, 224
BsiSI CCGG 1 cut(s) 26
BslI CCNNNNNNNGG 1 cut(s) 32
Bsp143I GATC 1 cut(s) 123
BspHI TCATGA 1 cut(s) 274
BsrI ACTGG 1 cut(s) 94
BssMI GATC 1 cut(s) 123
BssNAI GTATAC 1 cut(s) 78
Bst1107I GTATAC 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 244
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 1 cut(s) 126
BstMBI GATC 1 cut(s) 123
BstMWI GCNNNNNNNGC 1 cut(s) 97
BstNI CCWGG 1 cut(s) 244
BstSCI CCNGG 1 cut(s) 242
BstSFI CTRYAG 1 cut(s) 108
BstV1I GCAGC 2 cut(s) 158, 224
BstXI CCANNNNNNTGG 1 cut(s) 101
BstZ17I GTATAC 1 cut(s) 78
BtsIMutI CAGTG 2 cut(s) 45, 101
CciI TCATGA 1 cut(s) 274
CfoI GCGC 1 cut(s) 242
CviAII CATG 3 cut(s) 164, 226, 275
CviJI RGCY 6 cut(s) 25, 91, 100, 107, 237, 287
CviKI_1 RGCY 6 cut(s) 25, 91, 100, 107, 237, 287
DpnI GATC 1 cut(s) 125
DpnII GATC 1 cut(s) 123
Eco57I CTGAAG 3 cut(s) 78, 192, 303
EcoRII CCWGG 1 cut(s) 242
FaeI CATG 3 cut(s) 167, 229, 278
FaiI YATR 7 cut(s) 78, 110, 165, 189, 227, 255, 276
FatI CATG 3 cut(s) 163, 225, 274
FblI GTMKAC 1 cut(s) 77
Fnu4HI GCNGC 2 cut(s) 147, 238
Fsp4HI GCNGC 2 cut(s) 147, 238
FspBI CTAG 2 cut(s) 15, 288
GlaI GCGC 1 cut(s) 241
GluI GCNGC 2 cut(s) 147, 238
HapII CCGG 1 cut(s) 26
HhaI GCGC 1 cut(s) 242
Hin1II CATG 3 cut(s) 167, 229, 278
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HinfI GANTC 4 cut(s) 167, 230, 278, 296
HpaI GTTAAC 1 cut(s) 223
HpaII CCGG 1 cut(s) 26
HphI GGTGA 1 cut(s) 47
Hpy166II GTNNAC 4 cut(s) 78, 115, 184, 223
Hpy188I TCNGA 2 cut(s) 172, 283
Hpy188III TCNNGA 2 cut(s) 140, 275
Hpy8I GTNNAC 4 cut(s) 78, 115, 184, 223
HpyAV CCTTC 1 cut(s) 53
HpyCH4V TGCA 3 cut(s) 11, 53, 86
HpyF10VI GCNNNNNNNGC 1 cut(s) 97
Hsp92II CATG 3 cut(s) 167, 229, 278
HspAI GCGC 1 cut(s) 240
KspAI GTTAAC 1 cut(s) 223
Kzo9I GATC 1 cut(s) 123
LmnI GCTCC 1 cut(s) 104
LpnPI CCDG 6 cut(s) 39, 107, 181, 219, 229, 256
Lsp1109I GCAGC 2 cut(s) 158, 224
LweI GCATC 3 cut(s) 20, 40, 158
MaeI CTAG 2 cut(s) 15, 288
MalI GATC 1 cut(s) 125
MboI GATC 1 cut(s) 123
MboII GAAGA 3 cut(s) 133, 211, 214
MlyI GAGTC 1 cut(s) 176
MnlI CCTC 3 cut(s) 31, 124, 136
MseI TTAA 2 cut(s) 222, 304
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 1 cut(s) 244
MvaI CCWGG 1 cut(s) 244
MwoI GCNNNNNNNGC 1 cut(s) 97
NdeII GATC 1 cut(s) 123
NlaIII CATG 3 cut(s) 167, 229, 278
PagI TCATGA 1 cut(s) 274
PfeI GAWTC 3 cut(s) 230, 278, 296
PkrI GCNGC 2 cut(s) 148, 239
PleI GAGTC 1 cut(s) 175
PpsI GAGTC 1 cut(s) 175
PsiI TTATAA 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 242
PspGI CCWGG 1 cut(s) 242
SaqAI TTAA 2 cut(s) 222, 304
SatI GCNGC 2 cut(s) 147, 238
Sau3AI GATC 1 cut(s) 123
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 1 cut(s) 244
SetI ASST 4 cut(s) 37, 42, 109, 289
SfaNI GCATC 3 cut(s) 20, 40, 158
SfcI CTRYAG 1 cut(s) 108
SspMI CTAG 2 cut(s) 15, 288
StyD4I CCNGG 1 cut(s) 242
TfiI GAWTC 3 cut(s) 230, 278, 296
Tru1I TTAA 2 cut(s) 222, 304
Tru9I TTAA 2 cut(s) 222, 304
TscAI CASTG 2 cut(s) 52, 101
TseI GCWGC 2 cut(s) 146, 237
TspDTI ATGAA 3 cut(s) 231, 263, 291
TspRI CASTG 2 cut(s) 52, 101
XmiI GTMKAC 1 cut(s) 77
XspI CTAG 2 cut(s) 15, 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.