Rh5BG318500

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002213
Physical Location & Seq
Reverse (-)
42494225 .. 42496395
2171 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG318500.1

Sequence Viewer

Length: 1101 bp
ATGGCTATGGAAATGGAAGAGGATTTGTCAAGCATGGTATCAAATGCAGGCACAACAAGGCAGAAGAGGATATATCAAGCAGAAGCTTCTGTTTCAAGCATCACTGGTTTCTTGGTCCAAGAGAGTAAGCGCTTGAGTCATGGGAGCAGCAATTTGTCTGCCAATACCAAATTTAAAGGAGTTGTACTGCAGCAAAATGGCCACTGGGGTGCTCAGATCTATGCCAATCACAATAGGGTTTGGCTTGGAACATTCAATTCTGAGGTGGATGCAGCCATGGCTTATGATAGTGCTGCAATCAAGCTTCGCAACGGAGAGTGCCAAAGGAACTTTGCATGGACCAAGGTCACTGATCAAGAGCCATACTTTCAGAATCTCTATAGCCAAGAAGCTGTTCTCAACATGATCAAGGACGGGACTTACCAGGCTAGGTTTTTAGAATTTCGCATGTCTCACCAGGCAGCAGAAAGTATGCAAAAAATAGGAATGGCCGAGTGTAACTCAGCTAGGGTGCAACAAAGCAAAAGAGATATCTTGCACAAACAACTCTTCCAAAAAGAGCTGACGCCAAGCGATGTTGGTAAGCTGAACAGACTTGTCATACCCAAAAATTATGCAACTAAATACTTCCCTCGTATTGATGAATCCATGCAGGAAGGGTTGGATGACAACCCGGCTCCTGATGTGCAGCTGTCATTTTATGATGAAAACATGAGGCCATGGAAGTTCAGGTACTGTTATTGGAAGAGCAGCCAAAGCTTTGTGTTCACCCGGGGTTGGAATCGGTTTGTAAAGGAGAGTCTTTTGAAAGCCAATGACATTATCAGGTTCTATGAGTGCCACATTAGGGAAGGAGACAAGGAGGCCAAGTCATTCTACATGATTAAAATCGACCGGGCTGAAAGTTCTGATGTTATTGTTGAAAATGCTGGCCTATGTGTTTCCCCCGCCCTTACCCCCTTGCATGCCAAGAAATTTGGAGAGGAGAAAGATGACATAAAGACGCAAATTGCAGAGGTTATTGATGAAACGCCACAAGTTAATGGTGATAATAAAAGAATTAGGCTCTTTGGTGTTTGTATGGTCGTAGGTGAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 B3

Protein Analysis

366

Amino Acids

41.94

Weight (kDa)

7.05

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 56 - 104 1.8e-06 AP2 domain
B3 PF02362 184 - 283 5.9e-21 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 948
AcoI YGGCCR 2 cut(s) 199, 489
AcsI RAATTY 3 cut(s) 170, 440, 974
AcyI GRCGYC 1 cut(s) 566
AfaI GTAC 2 cut(s) 186, 734
AfeI AGCGCT 1 cut(s) 131
AfiI CCNNNNNNNGG 2 cut(s) 777, 847
AgsI TTSAA 4 cut(s) 96, 256, 808, 923
AjnI CCWGG 2 cut(s) 423, 456
AjuI GAANNNNNNNTTGG 2 cut(s) 378, 410
AleI CACNNNNGTG 1 cut(s) 207
AluBI AGCT 8 cut(s) 86, 304, 392, 506, 562, 586, 691, 759
AluI AGCT 8 cut(s) 86, 304, 392, 506, 562, 586, 691, 759
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 2 cut(s) 456, 849
AlwNI CAGNNNCTG 1 cut(s) 735
Ama87I CYCGRG 1 cut(s) 771
Aor51HI AGCGCT 1 cut(s) 131
AoxI GGCC 5 cut(s) 199, 489, 716, 864, 931
ApeKI GCWGC 7 cut(s) 147, 190, 272, 293, 461, 688, 750
ApoI RAATTY 3 cut(s) 170, 440, 974
Asp700I GAANNNNTTC 1 cut(s) 393
AspLEI GCGC 1 cut(s) 132
AspS9I GGNCC 2 cut(s) 115, 339
AsuC2I CCSGG 4 cut(s) 674, 772, 773, 896
AsuHPI GGTGA 3 cut(s) 446, 760, 1058
AvaI CYCGRG 1 cut(s) 771
AvaII GGWCC 2 cut(s) 115, 339
BalI TGGCCA 1 cut(s) 201
BarI GAAGNNNNNNTAC 2 cut(s) 716, 748
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 7 cut(s) 159, 202, 280, 284, 473, 700, 762
BciT130I CCWGG 2 cut(s) 425, 458
BclI TGATCA 2 cut(s) 352, 405
BcnI CCSGG 4 cut(s) 674, 772, 773, 896
BcoDI GTCTC 2 cut(s) 456, 849
BfaI CTAG 2 cut(s) 429, 507
BfmI CTRYAG 2 cut(s) 188, 379
BfoI RGCGCY 1 cut(s) 133
BglII AGATCT 1 cut(s) 216
BisI GCNGC 7 cut(s) 148, 191, 273, 294, 462, 689, 751
BlsI GCNGC 7 cut(s) 149, 192, 274, 295, 463, 690, 752
Bme1390I CCNGG 6 cut(s) 425, 458, 674, 772, 773, 896
Bme18I GGWCC 2 cut(s) 115, 339
BmeT110I CYCGRG 1 cut(s) 771
BmgT120I GGNCC 2 cut(s) 115, 339
BmiI GGNNCC 1 cut(s) 678
BmrFI CCNGG 6 cut(s) 425, 458, 674, 772, 773, 896
BmrI ACTGGG 1 cut(s) 214
BmsI GCATC 2 cut(s) 108, 259
BmuI ACTGGG 1 cut(s) 214
BoxI GACNNNNGTC 1 cut(s) 344
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BpuEI CTTGAG 1 cut(s) 154
BpuMI CCSGG 4 cut(s) 674, 772, 773, 896
BsaBI GATNNNNATC 1 cut(s) 887
BsaHI GRCGYC 1 cut(s) 566
BsaJI CCNNGG 5 cut(s) 276, 342, 719, 771, 772
BsaXI ACNNNNNCTCC 2 cut(s) 854, 884
Bsc4I CCNNNNNNNGG 2 cut(s) 777, 847
Bse1I ACTGG 2 cut(s) 109, 209
Bse8I GATNNNNATC 1 cut(s) 887
BseBI CCWGG 2 cut(s) 425, 458
BseDI CCNNGG 5 cut(s) 276, 342, 719, 771, 772
BseGI GGATG 2 cut(s) 274, 670
BseJI GATNNNNATC 1 cut(s) 887
BseLI CCNNNNNNNGG 2 cut(s) 777, 847
BseMII CTCAG 3 cut(s) 227, 252, 516
BseNI ACTGG 2 cut(s) 109, 209
BseRI GAGGAG 1 cut(s) 998
BseXI GCAGC 7 cut(s) 159, 202, 280, 284, 473, 700, 762
BsgI GTGCAG 1 cut(s) 707
Bsh1285I CGRYCG 1 cut(s) 895
BshFI GGCC 5 cut(s) 201, 491, 718, 866, 933
BsiEI CGRYCG 1 cut(s) 895
BsiHKAI GWGCWC 1 cut(s) 214
BsiHKCI CYCGRG 1 cut(s) 771
BsiSI CCGG 3 cut(s) 674, 772, 895
BslFI GGGAC 1 cut(s) 430
BslI CCNNNNNNNGG 2 cut(s) 777, 847
BsmAI GTCTC 2 cut(s) 456, 849
BsmFI GGGAC 1 cut(s) 430
BsnI GGCC 5 cut(s) 201, 491, 718, 866, 933
BsoBI CYCGRG 1 cut(s) 771
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 3 cut(s) 216, 352, 405
Bsp19I CCATGG 2 cut(s) 276, 719
BspACI CCGC 1 cut(s) 948
BspANI GGCC 5 cut(s) 201, 491, 718, 866, 933
BspCNI CTCAG 3 cut(s) 226, 253, 515
BspLI GGNNCC 1 cut(s) 678
BspMAI CTGCAG 1 cut(s) 192
BspQI GCTCTTC 1 cut(s) 740
BsrI ACTGG 2 cut(s) 109, 209
BssECI CCNNGG 5 cut(s) 276, 342, 719, 771, 772
BssMI GATC 3 cut(s) 216, 352, 405
BssNI GRCGYC 1 cut(s) 566
BssT1I CCWWGG 3 cut(s) 276, 342, 719
Bst2UI CCWGG 2 cut(s) 425, 458
Bst4CI ACNGT 1 cut(s) 737
Bst6I CTCTTC 4 cut(s) 12, 59, 554, 740
BstACI GRCGYC 1 cut(s) 566
BstC8I GCNNGC 3 cut(s) 49, 931, 966
BstDEI CTNAG 3 cut(s) 213, 261, 502
BstDSI CCRYGG 2 cut(s) 276, 719
BstF5I GGATG 2 cut(s) 274, 670
BstH2I RGCGCY 1 cut(s) 133
BstHHI GCGC 1 cut(s) 132
BstKTI GATC 3 cut(s) 219, 355, 408
BstMAI GTCTC 2 cut(s) 456, 849
BstMBI GATC 3 cut(s) 216, 352, 405
BstMCI CGRYCG 1 cut(s) 895
BstMWI GCNNNNNNNGC 2 cut(s) 278, 756
BstNI CCWGG 2 cut(s) 425, 458
BstNSI RCATGY 2 cut(s) 451, 968
BstPAI GACNNNNGTC 1 cut(s) 344
BstSCI CCNGG 6 cut(s) 423, 456, 672, 770, 771, 894
BstSFI CTRYAG 2 cut(s) 188, 379
BstV1I GCAGC 7 cut(s) 159, 202, 280, 284, 473, 700, 762
BstX2I RGATCY 1 cut(s) 216
BstYI RGATCY 1 cut(s) 216
BsuRI GGCC 5 cut(s) 201, 491, 718, 866, 933
BtgI CCRYGG 2 cut(s) 276, 719
BtgZI GCGATG 1 cut(s) 588
BtsCI GGATG 2 cut(s) 274, 670
BtsIMutI CAGTG 3 cut(s) 102, 202, 348
Cac8I GCNNGC 3 cut(s) 49, 931, 966
CaiI CAGNNNCTG 1 cut(s) 735
CfoI GCGC 1 cut(s) 132
Cfr13I GGNCC 2 cut(s) 115, 339
Cfr9I CCCGGG 1 cut(s) 771
CseI GACGC 2 cut(s) 574, 1012
Csp6I GTAC 2 cut(s) 185, 733
CviQI GTAC 2 cut(s) 185, 733
DdeI CTNAG 3 cut(s) 213, 261, 502
DpnI GATC 3 cut(s) 218, 354, 407
DpnII GATC 3 cut(s) 216, 352, 405
DraI TTTAAA 1 cut(s) 175
EaeI YGGCCR 2 cut(s) 199, 489
Eam1104I CTCTTC 4 cut(s) 12, 59, 554, 740
EarI CTCTTC 4 cut(s) 12, 59, 554, 740
Eco130I CCWWGG 3 cut(s) 276, 342, 719
Eco32I GATATC 1 cut(s) 532
Eco47I GGWCC 2 cut(s) 115, 339
Eco47III AGCGCT 1 cut(s) 131
Eco88I CYCGRG 1 cut(s) 771
EcoRII CCWGG 2 cut(s) 423, 456
EcoRV GATATC 1 cut(s) 532
EcoT14I CCWWGG 3 cut(s) 276, 342, 719
ErhI CCWWGG 3 cut(s) 276, 342, 719
FaqI GGGAC 1 cut(s) 430
FauI CCCGC 1 cut(s) 955
FbaI TGATCA 2 cut(s) 352, 405
Fnu4HI GCNGC 7 cut(s) 148, 191, 273, 294, 462, 689, 751
FokI GGATG 2 cut(s) 281, 677
Fsp4HI GCNGC 7 cut(s) 148, 191, 273, 294, 462, 689, 751
FspBI CTAG 2 cut(s) 429, 507
GlaI GCGC 1 cut(s) 131
GluI GCNGC 7 cut(s) 148, 191, 273, 294, 462, 689, 751
HaeII RGCGCY 1 cut(s) 133
HaeIII GGCC 5 cut(s) 201, 491, 718, 866, 933
HapII CCGG 3 cut(s) 674, 772, 895
HgaI GACGC 2 cut(s) 574, 1012
HhaI GCGC 1 cut(s) 132
Hin1I GRCGYC 1 cut(s) 566
Hin6I GCGC 1 cut(s) 130
HinP1I GCGC 1 cut(s) 130
HindIII AAGCTT 3 cut(s) 84, 302, 757
HinfI GANTC 5 cut(s) 136, 373, 644, 781, 799
HpaII CCGG 3 cut(s) 674, 772, 895
HphI GGTGA 3 cut(s) 446, 760, 1058
Hpy166II GTNNAC 1 cut(s) 768
Hpy188I TCNGA 4 cut(s) 216, 262, 372, 910
Hpy188III TCNNGA 2 cut(s) 356, 680
Hpy8I GTNNAC 1 cut(s) 768
HpyAV CCTTC 2 cut(s) 650, 845
HpyCH4III ACNGT 1 cut(s) 737
HpyF10VI GCNNNNNNNGC 2 cut(s) 278, 756
HpyF3I CTNAG 3 cut(s) 213, 261, 502
Hsp92I GRCGYC 1 cut(s) 566
HspAI GCGC 1 cut(s) 130
Ksp22I TGATCA 2 cut(s) 352, 405
Kzo9I GATC 3 cut(s) 216, 352, 405
LguI GCTCTTC 1 cut(s) 740
LmnI GCTCC 2 cut(s) 144, 682
Lsp1109I GCAGC 7 cut(s) 159, 202, 280, 284, 473, 700, 762
LweI GCATC 2 cut(s) 108, 259
MaeI CTAG 2 cut(s) 429, 507
MaeIII GTNAC 2 cut(s) 346, 497
MalI GATC 3 cut(s) 218, 354, 407
MboI GATC 3 cut(s) 216, 352, 405
MboII GAAGA 4 cut(s) 29, 76, 541, 757
MflI RGATCY 1 cut(s) 216
MhlI GDGCHC 1 cut(s) 214
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 9 cut(s) 151, 170, 256, 440, 610, 974, 1008, 1059, 1096
MluNI TGGCCA 1 cut(s) 201
MlyI GAGTC 2 cut(s) 145, 808
MmeI TCCRAC 2 cut(s) 642, 758
MnlI CCTC 8 cut(s) 13, 60, 256, 642, 708, 856, 976, 1009
Mox20I TGGCCA 1 cut(s) 201
MroXI GAANNNNTTC 1 cut(s) 393
MscI TGGCCA 1 cut(s) 201
MseI TTAA 4 cut(s) 174, 885, 1041, 1099
MslI CAYNNNNRTG 1 cut(s) 207
Msp20I TGGCCA 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 691
MspI CCGG 3 cut(s) 674, 772, 895
MspR9I CCNGG 6 cut(s) 425, 458, 674, 772, 773, 896
MvaI CCWGG 2 cut(s) 425, 458
MwoI GCNNNNNNNGC 2 cut(s) 278, 756
NciI CCSGG 4 cut(s) 674, 772, 773, 896
NcoI CCATGG 2 cut(s) 276, 719
NdeII GATC 3 cut(s) 216, 352, 405
NlaIV GGNNCC 1 cut(s) 678
NmeAIII GCCGAG 1 cut(s) 517
NmuCI GTSAC 1 cut(s) 346
NspI RCATGY 2 cut(s) 451, 968
OliI CACNNNNGTG 1 cut(s) 207
PaeI GCATGC 1 cut(s) 968
PciSI GCTCTTC 1 cut(s) 740
PdmI GAANNNNTTC 1 cut(s) 393
PfeI GAWTC 3 cut(s) 373, 644, 781
PkrI GCNGC 7 cut(s) 149, 192, 274, 295, 463, 690, 752
PleI GAGTC 2 cut(s) 144, 807
PpsI GAGTC 2 cut(s) 144, 807
PshAI GACNNNNGTC 1 cut(s) 344
Psp6I CCWGG 2 cut(s) 423, 456
PspGI CCWGG 2 cut(s) 423, 456
PspN4I GGNNCC 1 cut(s) 678
PspPI GGNCC 2 cut(s) 115, 339
PstI CTGCAG 1 cut(s) 192
PstNI CAGNNNCTG 1 cut(s) 735
PsuI RGATCY 1 cut(s) 216
PvuII CAGCTG 1 cut(s) 691
RsaI GTAC 2 cut(s) 186, 734
RsaNI GTAC 2 cut(s) 185, 733
RseI CAYNNNNRTG 1 cut(s) 207
SapI GCTCTTC 1 cut(s) 740
SaqAI TTAA 4 cut(s) 174, 885, 1041, 1099
SatI GCNGC 7 cut(s) 148, 191, 273, 294, 462, 689, 751
Sau3AI GATC 3 cut(s) 216, 352, 405
Sau96I GGNCC 2 cut(s) 115, 339
SchI GAGTC 2 cut(s) 145, 808
ScrFI CCNGG 6 cut(s) 425, 458, 674, 772, 773, 896
SduI GDGCHC 1 cut(s) 214
SfaNI GCATC 2 cut(s) 108, 259
SfcI CTRYAG 2 cut(s) 188, 379
SinI GGWCC 2 cut(s) 115, 339
SmaI CCCGGG 1 cut(s) 773
SmiMI CAYNNNNRTG 1 cut(s) 207
SmlI CTYRAG 1 cut(s) 133
SmoI CTYRAG 1 cut(s) 133
SphI GCATGC 1 cut(s) 968
Sse9I AATT 9 cut(s) 151, 170, 256, 440, 610, 974, 1008, 1059, 1096
SsiI CCGC 1 cut(s) 948
SspMI CTAG 2 cut(s) 429, 507
StyD4I CCNGG 6 cut(s) 423, 456, 672, 770, 771, 894
StyI CCWWGG 3 cut(s) 276, 342, 719
TaaI ACNGT 1 cut(s) 737
TaqI TCGA 1 cut(s) 891
TasI AATT 9 cut(s) 151, 170, 256, 440, 610, 974, 1008, 1059, 1096
TatI WGTACW 1 cut(s) 184
TfiI GAWTC 3 cut(s) 373, 644, 781
Tru1I TTAA 4 cut(s) 174, 885, 1041, 1099
Tru9I TTAA 4 cut(s) 174, 885, 1041, 1099
TscAI CASTG 3 cut(s) 109, 209, 355
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 7 cut(s) 147, 190, 272, 293, 461, 688, 750
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 3 cut(s) 657, 720, 1041
TspGWI ACGGA 1 cut(s) 327
TspMI CCCGGG 1 cut(s) 771
TspRI CASTG 3 cut(s) 109, 209, 355
VpaK11BI GGWCC 2 cut(s) 115, 339
XapI RAATTY 3 cut(s) 170, 440, 974
XceI RCATGY 2 cut(s) 451, 968
XmaI CCCGGG 1 cut(s) 771
XmnI GAANNNNTTC 1 cut(s) 393
XspI CTAG 2 cut(s) 429, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.