Rw7G035560

B3 DNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0002213
Physical Location & Seq
Forward (+)
53262062 .. 53262880
819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G035560.1

Sequence Viewer

Length: 600 bp
ATGGTTTTGGAAATGGAAGAGGATTTGTCAAGTACGGTCTCAAACGCAGGCACGACAACAAGGCGGAAGAGGACATATCAAGCAGAAGCTTCTATTTCAAGTATCACTGGTTTCGTTCAAGAGAGTAAGCGCTTGAGTCATGGGAGCAACAATTTGTCTACCAATGCCAAATTTAAAGGAGTTCTACTGCAAATTGGCCACTGGGGTGCTCAGATCTATGCCATTCACAATAGGGTTTGGCTTGGAATATTCAATTCCGAGGTGGACGCAGCCATGTCTTATGATAGTGCTGCAATCAAGCTTCGCAACGGAGAGTGCCAAAAGGAACTTTGCATGGACCAAGATATCTTTCACAAACAACTCTTTCAAAAAGAGCTGACGCCAAGCGATGTTAACAGACTCGTCATTCCCAAAAATTACGCAACTAAATACTTCCCTCGTATTGATGAATCCATGCACGGAGGGTTGGATGACAACCCGGCGCCTGATAAGTTCAGGTACTCTTACTGGAAGAGCAGCCAAAGCTTTGTGTTCACCCGGGGTTGGAATCGGTTTCATTACCTCCTTGCATGCCAAGAAGTTTGGAGAGGAGAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 B3

Protein Analysis

199

Amino Acids

22.9

Weight (kDa)

8.72

Isoelectric Point (pI)

49.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 122 - 185 3.9e-07 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 481
AccI GTMKAC 1 cut(s) 158
AciI CCGC 1 cut(s) 64
AcoI YGGCCR 1 cut(s) 196
AcsI RAATTY 1 cut(s) 170
AcyI GRCGYC 2 cut(s) 380, 482
AfaI GTAC 2 cut(s) 34, 500
AfeI AGCGCT 1 cut(s) 131
AfiI CCNNNNNNNGG 1 cut(s) 543
AgsI TTSAA 4 cut(s) 99, 119, 253, 368
AjuI GAANNNNNNNTTGG 2 cut(s) 333, 365
AleI CACNNNNGTG 1 cut(s) 204
AluBI AGCT 4 cut(s) 89, 301, 376, 525
AluI AGCT 4 cut(s) 89, 301, 376, 525
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 537
Aor51HI AGCGCT 1 cut(s) 131
AoxI GGCC 1 cut(s) 196
ApeKI GCWGC 3 cut(s) 269, 290, 516
ApoI RAATTY 1 cut(s) 170
AspLEI GCGC 2 cut(s) 132, 484
AspS9I GGNCC 1 cut(s) 337
AsuC2I CCSGG 3 cut(s) 479, 538, 539
AsuHPI GGTGA 1 cut(s) 526
AvaI CYCGRG 1 cut(s) 537
AvaII GGWCC 1 cut(s) 337
BalI TGGCCA 1 cut(s) 198
BanI GGYRCC 1 cut(s) 481
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 3 cut(s) 277, 281, 528
BcnI CCSGG 3 cut(s) 479, 538, 539
BcoDI GTCTC 1 cut(s) 43
BfoI RGCGCY 2 cut(s) 133, 485
BglII AGATCT 1 cut(s) 213
BisI GCNGC 3 cut(s) 270, 291, 517
BlsI GCNGC 3 cut(s) 271, 292, 518
Bme1390I CCNGG 3 cut(s) 479, 538, 539
Bme18I GGWCC 1 cut(s) 337
BmeT110I CYCGRG 1 cut(s) 537
BmgT120I GGNCC 1 cut(s) 337
BmiI GGNNCC 1 cut(s) 483
BmrFI CCNGG 3 cut(s) 479, 538, 539
BmrI ACTGGG 1 cut(s) 211
BmuI ACTGGG 1 cut(s) 211
BpuEI CTTGAG 1 cut(s) 154
BpuMI CCSGG 3 cut(s) 479, 538, 539
BsaHI GRCGYC 2 cut(s) 380, 482
BsaI GGTCTC 1 cut(s) 43
BsaJI CCNNGG 3 cut(s) 258, 537, 538
Bsc4I CCNNNNNNNGG 1 cut(s) 543
Bse1I ACTGG 3 cut(s) 112, 206, 512
BseDI CCNNGG 3 cut(s) 258, 537, 538
BseGI GGATG 1 cut(s) 475
BseLI CCNNNNNNNGG 1 cut(s) 543
BseMII CTCAG 1 cut(s) 224
BseNI ACTGG 3 cut(s) 112, 206, 512
BseXI GCAGC 3 cut(s) 277, 281, 528
BshFI GGCC 1 cut(s) 198
BshNI GGYRCC 1 cut(s) 481
BsiHKAI GWGCWC 1 cut(s) 211
BsiHKCI CYCGRG 1 cut(s) 537
BsiSI CCGG 2 cut(s) 479, 538
BslI CCNNNNNNNGG 1 cut(s) 543
BsmAI GTCTC 1 cut(s) 43
BsnI GGCC 1 cut(s) 198
Bso31I GGTCTC 1 cut(s) 43
BsoBI CYCGRG 1 cut(s) 537
Bsp1286I GDGCHC 1 cut(s) 211
Bsp143I GATC 1 cut(s) 213
BspACI CCGC 1 cut(s) 64
BspANI GGCC 1 cut(s) 198
BspCNI CTCAG 1 cut(s) 223
BspLI GGNNCC 1 cut(s) 483
BspQI GCTCTTC 1 cut(s) 506
BspT107I GGYRCC 1 cut(s) 481
BspTNI GGTCTC 1 cut(s) 43
BsrI ACTGG 3 cut(s) 112, 206, 512
BssECI CCNNGG 3 cut(s) 258, 537, 538
BssMI GATC 1 cut(s) 213
BssNI GRCGYC 2 cut(s) 380, 482
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 3 cut(s) 12, 62, 506
BstACI GRCGYC 2 cut(s) 380, 482
BstC8I GCNNGC 2 cut(s) 49, 571
BstDEI CTNAG 1 cut(s) 210
BstF5I GGATG 1 cut(s) 475
BstH2I RGCGCY 2 cut(s) 133, 485
BstHHI GCGC 2 cut(s) 132, 484
BstKTI GATC 1 cut(s) 216
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 1 cut(s) 213
BstMWI GCNNNNNNNGC 1 cut(s) 522
BstNSI RCATGY 1 cut(s) 573
BstSCI CCNGG 3 cut(s) 477, 536, 537
BstV1I GCAGC 3 cut(s) 277, 281, 528
BstX2I RGATCY 1 cut(s) 213
BstYI RGATCY 1 cut(s) 213
BsuRI GGCC 1 cut(s) 198
BtgZI GCGATG 1 cut(s) 402
BtsCI GGATG 1 cut(s) 475
BtsIMutI CAGTG 2 cut(s) 105, 199
Cac8I GCNNGC 2 cut(s) 49, 571
CfoI GCGC 2 cut(s) 132, 484
Cfr13I GGNCC 1 cut(s) 337
Cfr9I CCCGGG 1 cut(s) 537
CseI GACGC 2 cut(s) 275, 388
Csp6I GTAC 2 cut(s) 33, 499
CviAII CATG 5 cut(s) 140, 274, 334, 454, 570
CviJI RGCY 8 cut(s) 89, 198, 241, 272, 301, 376, 519, 525
CviKI_1 RGCY 8 cut(s) 89, 198, 241, 272, 301, 376, 519, 525
CviQI GTAC 2 cut(s) 33, 499
DdeI CTNAG 1 cut(s) 210
DinI GGCGCC 1 cut(s) 483
DpnI GATC 1 cut(s) 215
DpnII GATC 1 cut(s) 213
DraI TTTAAA 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 196
Eam1104I CTCTTC 3 cut(s) 12, 62, 506
EarI CTCTTC 3 cut(s) 12, 62, 506
EciI GGCGGA 1 cut(s) 79
Eco31I GGTCTC 1 cut(s) 43
Eco32I GATATC 1 cut(s) 346
Eco47I GGWCC 1 cut(s) 337
Eco47III AGCGCT 1 cut(s) 131
Eco88I CYCGRG 1 cut(s) 537
EcoRV GATATC 1 cut(s) 346
EgeI GGCGCC 1 cut(s) 483
EheI GGCGCC 1 cut(s) 483
FaeI CATG 5 cut(s) 143, 277, 337, 457, 573
FaiI YATR 8 cut(s) 76, 141, 219, 275, 282, 335, 455, 571
FatI CATG 5 cut(s) 139, 273, 333, 453, 569
FblI GTMKAC 1 cut(s) 158
Fnu4HI GCNGC 3 cut(s) 270, 291, 517
FokI GGATG 1 cut(s) 482
Fsp4HI GCNGC 3 cut(s) 270, 291, 517
GlaI GCGC 2 cut(s) 131, 483
GluI GCNGC 3 cut(s) 270, 291, 517
HaeII RGCGCY 2 cut(s) 133, 485
HaeIII GGCC 1 cut(s) 198
HapII CCGG 2 cut(s) 479, 538
HgaI GACGC 2 cut(s) 275, 388
HhaI GCGC 2 cut(s) 132, 484
Hin1I GRCGYC 2 cut(s) 380, 482
Hin1II CATG 5 cut(s) 143, 277, 337, 457, 573
Hin6I GCGC 2 cut(s) 130, 482
HinP1I GCGC 2 cut(s) 130, 482
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HindIII AAGCTT 3 cut(s) 87, 299, 523
HinfI GANTC 4 cut(s) 136, 399, 449, 547
HpaI GTTAAC 1 cut(s) 394
HpaII CCGG 2 cut(s) 479, 538
HphI GGTGA 1 cut(s) 526
Hpy166II GTNNAC 4 cut(s) 159, 265, 394, 534
Hpy188I TCNGA 2 cut(s) 213, 259
Hpy188III TCNNGA 1 cut(s) 119
Hpy8I GTNNAC 4 cut(s) 159, 265, 394, 534
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4V TGCA 5 cut(s) 190, 293, 333, 457, 569
HpyF10VI GCNNNNNNNGC 1 cut(s) 522
HpyF3I CTNAG 1 cut(s) 210
Hsp92I GRCGYC 2 cut(s) 380, 482
Hsp92II CATG 5 cut(s) 143, 277, 337, 457, 573
HspAI GCGC 2 cut(s) 130, 482
KasI GGCGCC 1 cut(s) 481
KspAI GTTAAC 1 cut(s) 394
Kzo9I GATC 1 cut(s) 213
LguI GCTCTTC 1 cut(s) 506
LmnI GCTCC 1 cut(s) 144
LpnPI CCDG 8 cut(s) 33, 93, 187, 481, 492, 493, 498, 551
Lsp1109I GCAGC 3 cut(s) 277, 281, 528
MalI GATC 1 cut(s) 215
MboI GATC 1 cut(s) 213
MboII GAAGA 3 cut(s) 29, 79, 523
MflI RGATCY 1 cut(s) 213
MhlI GDGCHC 1 cut(s) 211
MlsI TGGCCA 1 cut(s) 198
MluCI AATT 5 cut(s) 151, 170, 192, 253, 415
MluNI TGGCCA 1 cut(s) 198
Mly113I GGCGCC 1 cut(s) 482
MlyI GAGTC 2 cut(s) 145, 393
MmeI TCCRAC 2 cut(s) 447, 524
MnlI CCTC 7 cut(s) 13, 63, 253, 447, 455, 572, 581
Mox20I TGGCCA 1 cut(s) 198
MscI TGGCCA 1 cut(s) 198
MseI TTAA 2 cut(s) 174, 393
MslI CAYNNNNRTG 1 cut(s) 204
Msp20I TGGCCA 1 cut(s) 198
MspI CCGG 2 cut(s) 479, 538
MspR9I CCNGG 3 cut(s) 479, 538, 539
MwoI GCNNNNNNNGC 1 cut(s) 522
NarI GGCGCC 1 cut(s) 482
NciI CCSGG 3 cut(s) 479, 538, 539
NdeII GATC 1 cut(s) 213
NlaIII CATG 5 cut(s) 143, 277, 337, 457, 573
NlaIV GGNNCC 1 cut(s) 483
NspI RCATGY 1 cut(s) 573
OliI CACNNNNGTG 1 cut(s) 204
PaeI GCATGC 1 cut(s) 573
PciSI GCTCTTC 1 cut(s) 506
PfeI GAWTC 2 cut(s) 449, 547
PkrI GCNGC 3 cut(s) 271, 292, 518
PleI GAGTC 2 cut(s) 144, 393
PluTI GGCGCC 1 cut(s) 485
PpsI GAGTC 2 cut(s) 144, 393
PspN4I GGNNCC 1 cut(s) 483
PspPI GGNCC 1 cut(s) 337
PsuI RGATCY 1 cut(s) 213
RsaI GTAC 2 cut(s) 34, 500
RsaNI GTAC 2 cut(s) 33, 499
RseI CAYNNNNRTG 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 506
SaqAI TTAA 2 cut(s) 174, 393
SatI GCNGC 3 cut(s) 270, 291, 517
Sau3AI GATC 1 cut(s) 213
Sau96I GGNCC 1 cut(s) 337
SchI GAGTC 2 cut(s) 145, 393
ScrFI CCNGG 3 cut(s) 479, 538, 539
SduI GDGCHC 1 cut(s) 211
SetI ASST 7 cut(s) 91, 264, 303, 378, 500, 527, 564
SfoI GGCGCC 1 cut(s) 483
SinI GGWCC 1 cut(s) 337
SmaI CCCGGG 1 cut(s) 539
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 133
SmoI CTYRAG 1 cut(s) 133
SphI GCATGC 1 cut(s) 573
Sse9I AATT 5 cut(s) 151, 170, 192, 253, 415
SsiI CCGC 1 cut(s) 64
SspDI GGCGCC 1 cut(s) 481
SspI AATATT 1 cut(s) 249
StyD4I CCNGG 3 cut(s) 477, 536, 537
TaaI ACNGT 1 cut(s) 37
TasI AATT 5 cut(s) 151, 170, 192, 253, 415
TfiI GAWTC 2 cut(s) 449, 547
Tru1I TTAA 2 cut(s) 174, 393
Tru9I TTAA 2 cut(s) 174, 393
TscAI CASTG 2 cut(s) 112, 206
TseI GCWGC 3 cut(s) 269, 290, 516
TspDTI ATGAA 2 cut(s) 462, 545
TspGWI ACGGA 2 cut(s) 324, 474
TspMI CCCGGG 1 cut(s) 537
TspRI CASTG 2 cut(s) 112, 206
VpaK11BI GGWCC 1 cut(s) 337
XapI RAATTY 1 cut(s) 170
XceI RCATGY 1 cut(s) 573
XmaI CCCGGG 1 cut(s) 537
XmiI GTMKAC 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.