Rw2G003100

Nuclear pore complex protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001803
Physical Location & Seq
Reverse (-)
2949208 .. 2966147
16940 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003100.1

Sequence Viewer

Length: 5658 bp
ATGACGCCGAAGCAGCTCCTCGCGGTAATCGAATCGGCGCTACTAGGCCCGCTGCCTCCGAGCCCATCTCAGAGAGTGGAGCTCATGCACGCTATTCGAAGCTCTCTCCCGTCGCTCCAGTCCCTCCTCTCATATCCGCCTCCGAAGCCGTCGGACCGAGCTCAAGTTCAGTCCAAAGAAGTTAGGCTTCCGAATGGGCCACCGATTTTGCTTGATGACCAGGACGTCCAGATTGCTTTGAAGTTAAGTGATGACCTCCATCTCAACGAGATTGATTGTGTCCGTTTGATTGTCTCTGCAAATCAAGAGTGGGGCTTAATGGGAAGAGAGCCACTAGAAATTTTGCGCCTTGCAACAGGGCTCTGGTACACTGAGAGAAGAGATCTATTAACAGCTGTCTATACACTTTTAAGGGCTGTTGTGCTTGATCAGGGACTTGAAGCTGAAGTTGTATCTGACATTCAAAAATATCTGGAAAACATTATCAATAATGGGCTTAGACAGAGGTTAATATCTCTTATAAAGGAACTTAATCGAGAAGAACCAGCTGGCTTGGGCGGGCCTCATTCTGAACGTTATGTTCTCGATTCTAGAGGAGCTCTTGTTGTACGACAAGCTGTGGTTTCCCGGGAAAGGCTCATACTTGGACACTGCCTCGTTCTTTCTATTCTGGTTGTGCGGACAAGTCCAAAGGATGTAAATGATATATTTCTTGTGTATAAAGATAGTGCTTCTGAACTTAGTGGGACTAATGACACTATAAAACGCCAGATTACATTCAGTCTTCTTTTTTCTCTTGTGATCGCTTTCGTATCAGACGCTCTGAGTGCAGCACCTGATAAAGCATCTGTCCTGTCCCGGGATGCATCTTTTAGACATGAATTTCATGAAATCGTAATGGCAGCCGGAAATGATCCAAATGTTGAGGGCTTTGTCGGTACCATAAGGCTTGCTTGGGCTGTACACTTGATGTTAATACAAGATGCTCTTCCCGGAAGGGATGCTATTTCAAGTGCTTCATCGAGTGATTTGGGTTATCTCCAGTTGTGCTTAGAAGTGGTTTTTTCAAAGAACGTTTTCCAGTTTATATTAGATGAAGTTCTTAAAACTGCAGCTTATGAGAATGATGACGAGGATATAAGCTATATGTACAATGCTTATCTTCACAAGTTGATCACCTGCTTCTTATCTCACCCACTTGCCAGAGACAAGGTCAAAGAATCAAAGGAGAGGGCAATGAGCATGCTTAGTCCATACCGCCTGGTTGGATCACATGATTTTTTGCATGATAGTAACCAAACTTCACAAGCCTCTGAAAATGGTTCACTACCATTCGTCTCTCTTTTGGAGTTTGTGAGTGGAATTTATCAGAAGGAACCAGAGCTGTTGTCTGGCAATGATGTCTTGTGGACCTTTGTGAACTTTGCTGGAGAGGACCATACAAATTTTCAAACCCTTGTGGCATTCTTGAATATGCTGAGTACCTTGGCTTCTAGTCAAGAAGGTGCTGCAAAGGTTTTTGAGTTACTTCAGGGAAAAGTGTTTCGTTCTGTTGGTTGGAGCACTTTGTTTGATAGCTTATCAATTTATGACGAGAAGTTTAAACAGTCCCTTCAAACTGCTGGTGCCATGTTACCGGAACTCCCAGAGGGTGATGCAAAAGCACTCGTTGCTTATTTACATGTCCTTCAAAAGGTTGTGGAAAATGGAAATCCCCTTGAACGAAAGAACTGGTTTCCTGATATTGAGCCTTTGTTTAAGCTTCTTGGTTATGAAAATGTGCCTCCTTATCTAAAGGGTGCCTTGCGTAATGCAATAACAACTTTTGTATATGTCTCTCCTGTCCTAAAAGATACAGTCTGGAGTTATCTTGAGCAGTATGATCTCCCTGTGGTTGTTGGATCTCATGTTGGGAAGAGTGCACAGCCCATGGCTGCACAGGTGTATGATATGCAATTTGAGCTCAATGAAATAGAAGCAAGGAGAGAGCAATATCCTTCAACAATATCCTTCCTTAACCTGTTAAATGCTCTTATATCTGAAGAAAGAGACTTAAGTGATAGAGGGCGCAGATTCATTGGAATTTTCAGGTTCATTTATGATCATGTTTTTAGGCCATTCCCTCAAAGAGCCTATGCCGATCCTTGTGAGAAATGGCAATTAGTTGTTGCATGTCTTCAGCACTTTCATATGATGTTGAGTCTGTATGACATCAGTGAAGAGGATATTGATGGTGTCATTGATCAATCTCAGCTTTCAACAGTAACACAATCTTCACTCCAGATGCAGCTACCAATTCTAGAATTGCTAAAAGACTTTATGAGTGGAAAATCTGTTTTCCGGAACATCATGGGCATCCTTCTGCCAGGTGTCAATACCATTATAACTGAACGAACAAATCAAGTGTACGGGAAACTCTTAGAGAAGGCTGTGCAACTCTCATTGGAGATTATAATCCTTGTCTTGGAGAAGGATTTGCTTCTTTCTGATTTCTGGCGTCCTCTTTACCAGCCTCTGGATGTTATACTCTCTCAAGATCATAATCAAATTGTAGCTTTATTGGAATATGTCAGATATGATTTTCGACCACAAATTCAGCAGTGCTCTGTCAAAATCATGAGTATATTAAGTTCTCGCATGGTCGGGCTTGTACAATTACTGCTAAAGTCAAATGCTGCAAGCTGTTTGATTGAGGATTATGCAGCCTGCCTAGAATTACGATCAGAGGATTCTCAGGTGATAGAGAACGCTAGTGATGATCCAGGTGTACTAATATTGCAGCTTCTAATTGACAATATCAGTCGGCCAGCTCCCAATATCACCCATTTGCTACTCAAATTTGATCTTGACAGTCCCATTGAGCGTACTGTGCTACAACCAAAATTTCATTACAGTTGCTTGAAGGTTATCCTTGAGATCTTGGAGAAGCTTTCAAAGCCTGATGTGAATATGTTACTTCATGAATTTGGTTTTCAGCTCCTTTATAAATTGTGTGTGGATCCACTAACATGTGATCCTGCGATGGATCTGTTGAGTAGTAAGAAGTATCAGTTTTTTGTAAAGCACCTAGATACGATTGGGGTTGCCCCACTTCCTAAAAGGAAGAACAACCAGGCCCTACGCGTCAGTTCCCTTCATCAGAGAGCTTGGCTGTTGAAACTACTAGCTATTGAACTTCATGTTGGTGATGTCAATAAATCCACTCACCTAGAAACATCTCGTAGCATCCTTGCGCATCTTTTTGGGCAGGAAACTTTCGAAACTGGAATTGATCACCTCATGTCTCATTCGTTTTCTCCTCAAGATGGTGTTGAACATGCTGGAACCCAAACTGTCGGGAAGAGTAAGGTGTTGGAGTTGCTTGAAGTGGTTCAGTTTAGATCCCCGGATACAAACACGAAGCTTTCTGAGATTGTTTCCAGTACGAAATATGATTTGTTGGCAGAGGATGTAGTTTGTAATCCTACTTCTGGAAAGAGTGGTGTCCATTACTATTCGGAGCGTGGTGATCGATTGATAGACCTTGCGTCATTCCGTGATAAACTTTGGCAGAAATTCAATGCTGTTTATCCCCACTTGAGTAACATTGGAAGTGAAGCAGAGCTGAACGATGCAAAAGAAACAATTCAACAGTTGTTGAGATGGGGATGGAAGAACAATAAGAACGTTGAAGAGCAAGCTGGCCAACTCCATATGTTAACCGCCTGGTCGCAAGTTGTTGAGATCTCTGCTTCCCGAAGAATATCATCACTTGGACATCAATCTGAACTTCTATATCAGATTCTTGTAGCTGCTTTGACTGCTTCTGCTTCTCAAGATTGTTCATTAAAGATGGCATTTTTGTTGTGTCAGGTTGCATTGACATGCATGGCAAAGTTACGAGATGAAAGATTTTTATTCCCTGGTGGCTTTAGTTCTGATAATCAGGCATGTCTTGATATTATCATGGCAAAACAATTACCAAATGCAGCTTGTCATTCAATCCTGTTTAGGCTCATATTGGCAATTCTCAGACTTGAATCATCAGAAGCCTTAAGGAGACGGCAATACGCATTGCTCCTCAGCTATTTTCAGTATTGTCAGCATATGCTTGATCCAGATATCCCATCAACAGTTCTGCAATTTTTGTTGCTTGATGAGCAAGAGGGTGAAGATCTGGATCTCCAGAAGATCAATCGAGAACAAGCAGAACTTGCCCGTGCCAATTTCAATATTTTAAGAAAAGAAGCTCAATCTATTTTGGATTTGGTAATAAAAGATGCTACTCAAGGAAGTGAACTTGGGAAGACTATGGCACTCTATGTTTTGGATGCAATCATCTGTGTAGATCATGATAGATATTTCCTGGGCCAACTTCAAAGCAGGGGTTTCCTAAGATCTTGTCTTACAAACATCAGCAGCTTATCTTATCAGGATGGTGCACATTCTCGAGACTCAATGCAGCGAGCGCAAACTCTGGAGGCTGAACTTGCTTTGCTTTTGAGAATAAGCCATAAGTATGGGAAATCTGGAGCTCAGGTTATCTTTACAATGGGGGCTTTGGAGCATATTGCTTCATGCAGGGCGGTCAACGTTTTCCAGGGAAGTTTGCGATGGATTGACACTAAGAATCAAAGAAATGTTTCTGTCGATATCAACAAGCAAAGGATGATTGTAACCCCTATTCTGAGATTGGTGTTTTCTCTGTTGTCTCTAGTTGATACATCCGAATTTTATGAGGTGAAGAATAAAGTTGTTCGGGAAGTCATAGAATTTGTCAAGGGACATAGATCACTTTTTGATCATGTTCTTCGGGAAGACATTTCCCAAGCTGATGAGTTGGTGATGGAGCAGATAAACCTTGTTGTTGGTATACTAAGCAAGGTTTGGCCATATGAAGAGAGTGACGAATCTGGCTTTGTTCAAGGTCTTTTTTGTTTGATGCATGCTCTTTTCTCTCGTGACTGTGAGACTCTAAGTTCTGCTCAGTCAGTTCGATCTGTTGAGAATCACAGGAAAACAGAGCTTAACTCATTTCGAATATGCTTCAGCCTCAGCTCATATCTATATTTTCTAGTCACAAAGAAGTCCTTTAGACTGCAGGTCTCAGACATGCCTCCTGATTACAATGCTGCCGTTTCCTTTCAACAGCCAACATTGAGTTTGCTTGGATCTTTTCTCACTTCTTTAACAAGTGCTCTTGAAAGAGCTGCTGAGGAAAAATCCTTACTTCTTAATAGGATTCGAGACATAAATGAATTATCAAGGCAGGAGGTGGATGAAATTATCAATATGTATGCTCGGCAAGTATATGTTTCATCATCTGACAATATACAGAAAAGGAGGTATATTGCGATGGTGGAAATGTGCCATGTTGTCGGGAATAGAGATCAGTTAATAACTATATTACTTCCACTAGTGGAACATGTGCTGAATGTCTTCCTAAGCCATTTTCAGGACAGCTCTTTGGCATCTGATCCCGCTCGCTCTTTGAAGACAATTACATACGGTGCCAACTCTGGCCCAGAACAAGACATATCTTTGTTGTGCGGAAATCTTATTTTGACTTTAGAAAGACTCGAGTTACTTAGTGAGGACAAAATAGGCCACAATCTTAAAGTGTTTCGAAGATTAGTGACTTCGCTCAAGGAAATGACGATCCAAAAGTTGTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
Nup192

Protein Analysis

1885

Amino Acids

212.72

Weight (kDa)

5.6

Isoelectric Point (pI)

49.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nup192 PF11894 26 - 1606 4.9e-102 Nuclear pore complex scaffold, nucleoporins 186/192/205
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 521, 2384, 2453, 2985
AarI CACCTGC 1 cut(s) 1187
AasI GACNNNNNNGTC 1 cut(s) 224
AatII GACGTC 1 cut(s) 228
Acc16I TGCGCA 1 cut(s) 3234
Acc36I ACCTGC 2 cut(s) 1187, 5071
Acc65I GGTACC 1 cut(s) 938
AccB1I GGYRCC 4 cut(s) 938, 1625, 1799, 5489
AccB7I CCANNNNNTGG 1 cut(s) 2515
AccBSI CCGCTC 1 cut(s) 5462
AccI GTMKAC 1 cut(s) 4852
AccII CGCG 2 cut(s) 23, 3123
AccIII TCCGGA 1 cut(s) 2340
AclI AACGTT 4 cut(s) 574, 1074, 3666, 4572
AcoI YGGCCR 3 cut(s) 2804, 3682, 4868
AcuI CTGAAG 5 cut(s) 465, 1514, 2061, 2162, 5011
AcyI GRCGYC 3 cut(s) 5, 225, 2497
AfiI CCNNNNNNNGG 8 cut(s) 633, 859, 1693, 2464, 2515, 3305, 3470, 5434
AflII CTTAAG 2 cut(s) 2053, 4033
AflIII ACRYGT 4 cut(s) 1681, 3008, 3121, 5404
AhdI GACNNNNNGTC 2 cut(s) 3526, 5081
AhlI ACTAGT 1 cut(s) 5395
AjnI CCWGG 9 cut(s) 219, 1260, 2365, 2761, 3111, 3704, 3901, 4344, 4578
AjuI GAANNNNNNNTTGG 2 cut(s) 3165, 3197
Alw44I GTGCAC 2 cut(s) 1920, 4419
AlwNI CAGNNNCTG 2 cut(s) 836, 2515
Ama87I CYCGRG 4 cut(s) 627, 858, 4428, 5558
Aor13HI TCCGGA 1 cut(s) 2340
ApaLI GTGCAC 2 cut(s) 1920, 4419
Asp700I GAANNNNTTC 5 cut(s) 1076, 3369, 3624, 4621, 5417
Asp718I GGTACC 1 cut(s) 938
AspLEI GCGC 5 cut(s) 40, 348, 2070, 3235, 4450
AspS9I GGNCC 9 cut(s) 47, 154, 197, 560, 1410, 1435, 3115, 4348, 5501
AsuC2I CCSGG 6 cut(s) 628, 629, 859, 860, 993, 3386
AsuII TTCGAA 4 cut(s) 97, 3258, 5017, 5605
AvaI CYCGRG 4 cut(s) 627, 858, 4428, 5558
AvaII GGWCC 3 cut(s) 154, 1410, 1435
BaeGI GKGCMC 2 cut(s) 1924, 4423
BalI TGGCCA 2 cut(s) 3684, 4870
BamHI GGATCC 1 cut(s) 2998
BanI GGYRCC 4 cut(s) 938, 1625, 1799, 5489
BanII GRGCYC 7 cut(s) 65, 84, 163, 363, 601, 1965, 4516
BarI GAAGNNNNNNTAC 6 cut(s) 1285, 1317, 1474, 1506, 3451, 3483
BauI CACGAG 1 cut(s) 4938
BbsI GAAGAC 7 cut(s) 776, 2168, 4292, 4803, 5410, 5480, 5643
BbvCI CCTCAGC 3 cut(s) 4061, 5033, 5193
BceAI ACGGC 3 cut(s) 133, 4058, 5099
BcgI CGANNNNNNTGC 2 cut(s) 77, 111
BciT130I CCWGG 9 cut(s) 221, 1262, 2367, 2763, 3113, 3706, 3903, 4346, 4580
BciVI GTATCC 1 cut(s) 3382
BclI TGATCA 6 cut(s) 427, 1173, 2101, 2242, 3271, 4780
BcnI CCSGG 6 cut(s) 628, 629, 859, 860, 993, 3386
BcuI ACTAGT 1 cut(s) 5395
BfmI CTRYAG 2 cut(s) 1110, 5078
BfoI RGCGCY 1 cut(s) 41
BfrI CTTAAG 2 cut(s) 2053, 4033
BfuAI ACCTGC 2 cut(s) 1187, 5071
BfuI GTATCC 1 cut(s) 3382
BglII AGATCT 5 cut(s) 382, 2916, 3723, 4153, 4376
Bme18I GGWCC 3 cut(s) 154, 1410, 1435
BmeRI GACNNNNNGTC 2 cut(s) 3526, 5081
BmeT110I CYCGRG 4 cut(s) 627, 858, 4428, 5558
BmgT120I GGNCC 9 cut(s) 47, 154, 197, 560, 1410, 1435, 3115, 4348, 5501
BmiI GGNNCC 7 cut(s) 940, 1377, 1627, 1801, 3000, 3325, 5491
BpiI GAAGAC 7 cut(s) 776, 2168, 4292, 4803, 5410, 5480, 5643
BplI GAGNNNNNCTC 6 cut(s) 52, 84, 66, 98, 4994, 5026
BpmI CTGGAG 8 cut(s) 101, 1025, 1449, 1882, 2264, 4148, 4478, 4530
Bpu10I CCTNAGC 5 cut(s) 4061, 4515, 5033, 5193, 5423
Bpu14I TTCGAA 4 cut(s) 97, 3258, 5017, 5605
BpuEI CTTGAG 9 cut(s) 147, 1892, 2517, 2933, 3285, 3598, 3798, 4251, 5609
BpuMI CCSGG 6 cut(s) 628, 629, 859, 860, 993, 3386
Bsa29I ATCGAT 1 cut(s) 3511
BsaBI GATNNNNATC 3 cut(s) 2453, 2541, 4158
BsaHI GRCGYC 3 cut(s) 5, 225, 2497
BsaI GGTCTC 1 cut(s) 5089
BsaJI CCNNGG 8 cut(s) 627, 858, 1485, 1929, 3384, 3901, 4345, 4579
BsaWI WCCGGW 2 cut(s) 1636, 2340
BsaXI ACNNNNNCTCC 6 cut(s) 588, 618, 1626, 1656, 4503, 4533
Bsc4I CCNNNNNNNGG 8 cut(s) 633, 859, 1693, 2464, 2515, 3305, 3470, 5434
Bse1I ACTGG 6 cut(s) 118, 1042, 1081, 1736, 3268, 3420
Bse3DI GCAATG 3 cut(s) 1242, 1402, 4052
Bse8I GATNNNNATC 3 cut(s) 2453, 2541, 4158
BseAI TCCGGA 1 cut(s) 2340
BseBI CCWGG 9 cut(s) 221, 1262, 2367, 2763, 3113, 3706, 3903, 4346, 4580
BseCI ATCGAT 1 cut(s) 3511
BseDI CCNNGG 8 cut(s) 627, 858, 1485, 1929, 3384, 3901, 4345, 4579
BseJI GATNNNNATC 3 cut(s) 2453, 2541, 4158
BseLI CCNNNNNNNGG 8 cut(s) 633, 859, 1693, 2464, 2515, 3305, 3470, 5434
BseMI GCAATG 3 cut(s) 1242, 1402, 4052
BseNI ACTGG 6 cut(s) 118, 1042, 1081, 1736, 3268, 3420
BseRI GAGGAG 5 cut(s) 8, 116, 609, 3288, 4049
BseSI GKGCMC 2 cut(s) 1924, 4423
BsgI GTGCAG 2 cut(s) 849, 1920
Bsh1236I CGCG 2 cut(s) 23, 3123
BshNI GGYRCC 4 cut(s) 938, 1625, 1799, 5489
BshVI ATCGAT 1 cut(s) 3511
BsiHKCI CYCGRG 4 cut(s) 627, 858, 4428, 5558
BsiSI CCGG 7 cut(s) 628, 859, 906, 993, 1637, 2341, 3386
BslFI GGGAC 7 cut(s) 106, 447, 760, 841, 1594, 2838, 4776
BslI CCNNNNNNNGG 8 cut(s) 633, 859, 1693, 2464, 2515, 3305, 3470, 5434
BsmBI CGTCTC 2 cut(s) 1342, 4033
BsmFI GGGAC 7 cut(s) 106, 447, 760, 841, 1594, 2838, 4776
BsmI GAATGC 1 cut(s) 1463
Bso31I GGTCTC 1 cut(s) 5089
BsoBI CYCGRG 4 cut(s) 627, 858, 4428, 5558
Bsp119I TTCGAA 4 cut(s) 97, 3258, 5017, 5605
Bsp13I TCCGGA 1 cut(s) 2340
Bsp1407I TGTACA 3 cut(s) 961, 1149, 2650
Bsp19I CCATGG 1 cut(s) 1929
BspDI ATCGAT 1 cut(s) 3511
BspEI TCCGGA 1 cut(s) 2340
BspFNI CGCG 2 cut(s) 23, 3123
BspHI TCATGA 5 cut(s) 886, 2616, 2959, 4330, 5654
BspLI GGNNCC 7 cut(s) 940, 1377, 1627, 1801, 3000, 3325, 5491
BspMAI CTGCAG 2 cut(s) 1114, 5082
BspMI ACCTGC 2 cut(s) 1187, 5071
BspQI GCTCTTC 2 cut(s) 993, 3666
BspT104I TTCGAA 4 cut(s) 97, 3258, 5017, 5605
BspT107I GGYRCC 4 cut(s) 938, 1625, 1799, 5489
BspTI CTTAAG 2 cut(s) 2053, 4033
BspTNI GGTCTC 1 cut(s) 5089
BsrBI CCGCTC 1 cut(s) 5462
BsrDI GCAATG 3 cut(s) 1242, 1402, 4052
BsrGI TGTACA 3 cut(s) 961, 1149, 2650
BsrI ACTGG 6 cut(s) 118, 1042, 1081, 1736, 3268, 3420
BssECI CCNNGG 8 cut(s) 627, 858, 1485, 1929, 3384, 3901, 4345, 4579
BssNAI GTATAC 1 cut(s) 4853
BssNI GRCGYC 3 cut(s) 5, 225, 2497
BssSI CACGAG 1 cut(s) 4938
BssT1I CCWWGG 2 cut(s) 1485, 1929
Bst1107I GTATAC 1 cut(s) 4853
Bst2BI CACGAG 1 cut(s) 4938
Bst2UI CCWGG 9 cut(s) 221, 1262, 2367, 2763, 3113, 3706, 3903, 4346, 4580
Bst6I CTCTTC 8 cut(s) 319, 373, 993, 1910, 2214, 3335, 3666, 4872
BstACI GRCGYC 3 cut(s) 5, 225, 2497
BstAFI CTTAAG 2 cut(s) 2053, 4033
BstAPI GCANNNNNTGC 2 cut(s) 1670, 4193
BstAUI TGTACA 3 cut(s) 961, 1149, 2650
BstBI TTCGAA 4 cut(s) 97, 3258, 5017, 5605
BstDSI CCRYGG 1 cut(s) 1929
BstENI CCTNNNNNAGG 1 cut(s) 1691
BstFNI CGCG 2 cut(s) 23, 3123
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 5 cut(s) 40, 348, 2070, 3235, 4450
BstNI CCWGG 9 cut(s) 221, 1262, 2367, 2763, 3113, 3706, 3903, 4346, 4580
BstSFI CTRYAG 2 cut(s) 1110, 5078
BstSLI GKGCMC 2 cut(s) 1924, 4423
BstUI CGCG 2 cut(s) 23, 3123
BstV2I GAAGAC 7 cut(s) 776, 2168, 4292, 4803, 5410, 5480, 5643
BstXI CCANNNNNNTGG 1 cut(s) 4499
BstZ17I GTATAC 1 cut(s) 4853
Bsu15I ATCGAT 1 cut(s) 3511
BsuI GTATCC 1 cut(s) 3382
BsuTUI ATCGAT 1 cut(s) 3511
BtgI CCRYGG 1 cut(s) 1929
BtgZI GCGATG 3 cut(s) 3035, 4606, 5348
BtsI GCAGTG 2 cut(s) 649, 2606
BtsIMutI CAGTG 4 cut(s) 369, 649, 2221, 2606
BveI ACCTGC 2 cut(s) 1187, 5071
CaiI CAGNNNCTG 2 cut(s) 836, 2515
CciI TCATGA 5 cut(s) 886, 2616, 2959, 4330, 5654
CfoI GCGC 5 cut(s) 40, 348, 2070, 3235, 4450
Cfr13I GGNCC 9 cut(s) 47, 154, 197, 560, 1410, 1435, 3115, 4348, 5501
Cfr9I CCCGGG 2 cut(s) 627, 858
ClaI ATCGAT 1 cut(s) 3511
CpoI CGGWCCG 1 cut(s) 154
CseI GACGC 5 cut(s) 13, 827, 2486, 3112, 3516
CspCI CAANNNNNGTGG 2 cut(s) 189, 224
CspI CGGWCCG 1 cut(s) 154
DraI TTTAAA 1 cut(s) 1603
DrdI GACNNNNNNGTC 1 cut(s) 224
DriI GACNNNNNGTC 2 cut(s) 3526, 5081
DseDI GACNNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 3 cut(s) 2804, 3682, 4868
Eam1104I CTCTTC 8 cut(s) 319, 373, 993, 1910, 2214, 3335, 3666, 4872
Eam1105I GACNNNNNGTC 2 cut(s) 3526, 5081
EarI CTCTTC 8 cut(s) 319, 373, 993, 1910, 2214, 3335, 3666, 4872
EciI GGCGGA 1 cut(s) 126
Ecl136II GAGCTC 5 cut(s) 82, 161, 599, 1963, 4514
Eco130I CCWWGG 2 cut(s) 1485, 1929
Eco24I GRGCYC 7 cut(s) 65, 84, 163, 363, 601, 1965, 4516
Eco31I GGTCTC 1 cut(s) 5089
Eco32I GATATC 2 cut(s) 4102, 4633
Eco47I GGWCC 3 cut(s) 154, 1410, 1435
Eco53kI GAGCTC 5 cut(s) 82, 161, 599, 1963, 4514
Eco57I CTGAAG 5 cut(s) 465, 1514, 2061, 2162, 5011
Eco88I CYCGRG 4 cut(s) 627, 858, 4428, 5558
EcoICRI GAGCTC 5 cut(s) 82, 161, 599, 1963, 4514
EcoNI CCTNNNNNAGG 1 cut(s) 1691
EcoO109I RGGNCCY 1 cut(s) 3115
EcoRII CCWGG 9 cut(s) 219, 1260, 2365, 2761, 3111, 3704, 3901, 4344, 4578
EcoRV GATATC 2 cut(s) 4102, 4633
EcoT14I CCWWGG 2 cut(s) 1485, 1929
EcoT22I ATGCAT 3 cut(s) 868, 3869, 4926
EcoT38I GRGCYC 7 cut(s) 65, 84, 163, 363, 601, 1965, 4516
ErhI CCWWGG 2 cut(s) 1485, 1929
Esp3I CGTCTC 2 cut(s) 1342, 4033
FaqI GGGAC 7 cut(s) 106, 447, 760, 841, 1594, 2838, 4776
FauI CCCGC 3 cut(s) 57, 551, 5467
FauNDI CATATG 4 cut(s) 2190, 3693, 4086, 4873
FbaI TGATCA 6 cut(s) 427, 1173, 2101, 2242, 3271, 4780
FblI GTMKAC 1 cut(s) 4852
FriOI GRGCYC 7 cut(s) 65, 84, 163, 363, 601, 1965, 4516
FspI TGCGCA 1 cut(s) 3234
GlaI GCGC 5 cut(s) 39, 347, 2069, 3234, 4449
GsuI CTGGAG 8 cut(s) 101, 1025, 1449, 1882, 2264, 4148, 4478, 4530
HaeII RGCGCY 1 cut(s) 41
HapII CCGG 7 cut(s) 628, 859, 906, 993, 1637, 2341, 3386
HgaI GACGC 5 cut(s) 13, 827, 2486, 3112, 3516
HhaI GCGC 5 cut(s) 40, 348, 2070, 3235, 4450
Hin1I GRCGYC 3 cut(s) 5, 225, 2497
Hin6I GCGC 5 cut(s) 38, 346, 2068, 3233, 4448
HinP1I GCGC 5 cut(s) 38, 346, 2068, 3233, 4448
HincII GTYRAC 2 cut(s) 3699, 4570
HindII GTYRAC 2 cut(s) 3699, 4570
HindIII AAGCTT 3 cut(s) 1760, 2927, 3401
HpaI GTTAAC 1 cut(s) 3699
HpaII CCGG 7 cut(s) 628, 859, 906, 993, 1637, 2341, 3386
Hpy99I CGWCG 2 cut(s) 115, 154
HpyCH4IV ACGT 5 cut(s) 225, 574, 1074, 3666, 4572
HpySE526I ACGT 5 cut(s) 225, 574, 1074, 3666, 4572
Hsp92I GRCGYC 3 cut(s) 5, 225, 2497
HspAI GCGC 5 cut(s) 38, 346, 2068, 3233, 4448
Kpn2I TCCGGA 1 cut(s) 2340
KpnI GGTACC 1 cut(s) 942
Ksp22I TGATCA 6 cut(s) 427, 1173, 2101, 2242, 3271, 4780
KspAI GTTAAC 1 cut(s) 3699
LguI GCTCTTC 2 cut(s) 993, 3666
MaeII ACGT 5 cut(s) 225, 574, 1074, 3666, 4572
MbiI CCGCTC 1 cut(s) 5462
MlsI TGGCCA 2 cut(s) 3684, 4870
MluI ACGCGT 1 cut(s) 3121
MluNI TGGCCA 2 cut(s) 3684, 4870
MlyI GAGTC 4 cut(s) 2209, 4427, 4945, 5550
MmeI TCCRAC 5 cut(s) 132, 1246, 1538, 1879, 3333
Mox20I TGGCCA 2 cut(s) 3684, 4870
Mph1103I ATGCAT 3 cut(s) 868, 3869, 4926
MroI TCCGGA 1 cut(s) 2340
MroXI GAANNNNTTC 5 cut(s) 1076, 3369, 3624, 4621, 5417
MscI TGGCCA 2 cut(s) 3684, 4870
MslI CAYNNNNRTG 4 cut(s) 3007, 3183, 3862, 4497
Msp20I TGGCCA 2 cut(s) 3684, 4870
MspA1I CMGCKG 3 cut(s) 52, 395, 548
MspCI CTTAAG 2 cut(s) 2053, 4033
MspI CCGG 7 cut(s) 628, 859, 906, 993, 1637, 2341, 3386
MssI GTTTAAAC 1 cut(s) 1603
Mva1269I GAATGC 1 cut(s) 1463
MvaI CCWGG 9 cut(s) 221, 1262, 2367, 2763, 3113, 3706, 3903, 4346, 4580
MvnI CGCG 2 cut(s) 23, 3123
NciI CCSGG 6 cut(s) 628, 629, 859, 860, 993, 3386
NcoI CCATGG 1 cut(s) 1929
NdeI CATATG 4 cut(s) 2190, 3693, 4086, 4873
NlaIV GGNNCC 7 cut(s) 940, 1377, 1627, 1801, 3000, 3325, 5491
NmeAIII GCCGAG 1 cut(s) 5260
NmuCI GTSAC 4 cut(s) 4883, 4940, 5056, 5614
NsbI TGCGCA 1 cut(s) 3234
NsiI ATGCAT 3 cut(s) 868, 3869, 4926
NspV TTCGAA 4 cut(s) 97, 3258, 5017, 5605
PaeI GCATGC 2 cut(s) 1246, 4928
PaeR7I CTCGAG 2 cut(s) 4428, 5558
PagI TCATGA 5 cut(s) 886, 2616, 2959, 4330, 5654
PaqCI CACCTGC 1 cut(s) 1187
PciI ACATGT 3 cut(s) 1681, 3008, 5404
PciSI GCTCTTC 2 cut(s) 993, 3666
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PctI GAATGC 1 cut(s) 1463
PdmI GAANNNNTTC 5 cut(s) 1076, 3369, 3624, 4621, 5417
PflFI GACNNNGTC 1 cut(s) 1211
PflMI CCANNNNNTGG 1 cut(s) 2515
PfoI TCCNGGA 1 cut(s) 991
PleI GAGTC 4 cut(s) 2208, 4427, 4945, 5550
PmeI GTTTAAAC 1 cut(s) 1603
PpsI GAGTC 4 cut(s) 2208, 4427, 4945, 5550
PscI ACATGT 3 cut(s) 1681, 3008, 5404
PsiI TTATAA 4 cut(s) 521, 2384, 2453, 2985
Psp124BI GAGCTC 5 cut(s) 84, 163, 601, 1965, 4516
Psp1406I AACGTT 4 cut(s) 574, 1074, 3666, 4572
Psp6I CCWGG 9 cut(s) 219, 1260, 2365, 2761, 3111, 3704, 3901, 4344, 4578
PspGI CCWGG 9 cut(s) 219, 1260, 2365, 2761, 3111, 3704, 3901, 4344, 4578
PspN4I GGNNCC 7 cut(s) 940, 1377, 1627, 1801, 3000, 3325, 5491
PspPI GGNCC 9 cut(s) 47, 154, 197, 560, 1410, 1435, 3115, 4348, 5501
PspXI VCTCGAGB 1 cut(s) 5558
PsrI GAACNNNNNNTAC 2 cut(s) 2614, 2646
PstI CTGCAG 2 cut(s) 1114, 5082
PstNI CAGNNNCTG 2 cut(s) 836, 2515
PsyI GACNNNGTC 1 cut(s) 1211
PvuII CAGCTG 2 cut(s) 395, 548
RseI CAYNNNNRTG 4 cut(s) 3007, 3183, 3862, 4497
Rsr2I CGGWCCG 1 cut(s) 154
RsrII CGGWCCG 1 cut(s) 154
SacI GAGCTC 5 cut(s) 84, 163, 601, 1965, 4516
SapI GCTCTTC 2 cut(s) 993, 3666
Sau96I GGNCC 9 cut(s) 47, 154, 197, 560, 1410, 1435, 3115, 4348, 5501
SchI GAGTC 4 cut(s) 2209, 4427, 4945, 5550
SfcI CTRYAG 2 cut(s) 1110, 5078
Sfr274I CTCGAG 2 cut(s) 4428, 5558
SfuI TTCGAA 4 cut(s) 97, 3258, 5017, 5605
SinI GGWCC 3 cut(s) 154, 1410, 1435
SlaI CTCGAG 2 cut(s) 4428, 5558
SmaI CCCGGG 2 cut(s) 629, 860
SmiMI CAYNNNNRTG 4 cut(s) 3007, 3183, 3862, 4497
SpeI ACTAGT 1 cut(s) 5395
SphI GCATGC 2 cut(s) 1246, 4928
SspI AATATT 2 cut(s) 2775, 4213
SstI GAGCTC 5 cut(s) 84, 163, 601, 1965, 4516
StyI CCWWGG 2 cut(s) 1485, 1929
TaiI ACGT 5 cut(s) 228, 577, 1077, 3669, 4575
TaqII GACCGA 1 cut(s) 171
TatI WGTACW 4 cut(s) 961, 1149, 2650, 2767
TscAI CASTG 4 cut(s) 376, 656, 2221, 2606
TseFI GTSAC 4 cut(s) 4883, 4940, 5056, 5614
Tsp45I GTSAC 4 cut(s) 4883, 4940, 5056, 5614
TspGWI ACGGA 2 cut(s) 272, 3524
TspMI CCCGGG 2 cut(s) 627, 858
TspRI CASTG 4 cut(s) 376, 656, 2221, 2606
Tth111I GACNNNGTC 1 cut(s) 1211
Van91I CCANNNNNTGG 1 cut(s) 2515
Vha464I CTTAAG 2 cut(s) 2053, 4033
VneI GTGCAC 2 cut(s) 1920, 4419
VpaK11BI GGWCC 3 cut(s) 154, 1410, 1435
XagI CCTNNNNNAGG 1 cut(s) 1691
XbaI TCTAGA 2 cut(s) 590, 2299
XhoI CTCGAG 2 cut(s) 4428, 5558
XmaI CCCGGG 2 cut(s) 627, 858
XmiI GTMKAC 1 cut(s) 4852
XmnI GAANNNNTTC 5 cut(s) 1076, 3369, 3624, 4621, 5417
ZraI GACGTC 1 cut(s) 226
Zsp2I ATGCAT 3 cut(s) 868, 3869, 4926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.