Rw2G028520

Branched-chain-amino-acid aminotransferase-like protein 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001579
Physical Location & Seq
Forward (+)
45678053 .. 45680029
1977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G028520.1

Sequence Viewer

Length: 993 bp
ATGAAACAAATTCTCTCGAGCTCTCCTAGTTGTGAAACCCCCTTGCTTGAAAAGCTTCATGAGAAGTGGAGCAAAGTAGGAAAGAAACCATACCCAGCCATGTATTCCAGCATTTATGGTGGAATCATTCTTGATCCAGCTTTGATGGTGATTCCAATAGACGATCACATGGTTCATCGAGGCCATGGTGTGTTTGACACAGCCATTCTCTTAAATGGATACCTGTATGAGCTGGATGTCCATCTAAACCGCTTCCTCAGATCAGCTTCAAAAGCAAGGATCCCCTCTCCCTTTGATCGATCAACTCTTCGGAGCATTCTTGTGCAACTGGCGGCAGGGTCACAGATCAAGAAAGGAACTCTAAGATATTGGATGAGTGCCGGTTATGGTGATTTTTTGTTCCAACCTACAGGGTGCCCAACATCTGCATTCTATGCAGTAGTCATTGATGAGGACTTCAATCAGTGCAAAGAAGGGGATAAAGTGATCACTTCCTATATTCCCATGAAGTCACCAGAAATGGCGACAATGAAGAATGTGAACTACCTTCCGAATATCCTTGCAAAAATGGAAGCTGAAGACAAAGGAGCCTTTGCTTCCATATGGGTGGATGAGAAGGGTTACATAGCAAAAGGTCCAAATGTGAATGTAGCTTTTATAACACATGACAAGGAGCTTATTGCACCCTTCTTTGACAAGATCCTTGGTGGTTGTACCGTGTTAAGGCTTCTTGAACTAGCACCCAAGTTGGTTGAGTCGGGGCGTCTGAAAGGTGTGACAACTAGAAACATTAGTGTGGAAGAAGCCAAAAGCTCAGCGGAAATGATGTTTGTAGGAAGCACACTGCCACTGTTGCCAATTATCGCGTGGGACGAACAGCCCGTTGGCGATGGGAAGGTGGGAGAATTGACAATGGCACTCTCCGATCTGGTGTGGGATGATATGGTAGCAGGATATACTGAAACACAGAGGATAGCAGTTCCCTACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00790 map00790
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

330

Amino Acids

36.55

Weight (kDa)

5.92

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 63 - 289 3e-36 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 659
AccB1I GGYRCC 1 cut(s) 414
AccII CGCG 1 cut(s) 866
AciI CCGC 3 cut(s) 250, 332, 818
AclWI GGATC 4 cut(s) 128, 274, 287, 694
AcsI RAATTY 1 cut(s) 9
AcuI CTGAAG 1 cut(s) 597
AcyI GRCGYC 1 cut(s) 763
AfaI GTAC 1 cut(s) 715
AfiI CCNNNNNNNGG 1 cut(s) 723
AflIII ACRYGT 1 cut(s) 987
AgsI TTSAA 4 cut(s) 50, 270, 460, 734
AjuI GAANNNNNNNTTGG 2 cut(s) 867, 899
AluBI AGCT 9 cut(s) 21, 55, 140, 232, 266, 575, 653, 676, 813
AluI AGCT 9 cut(s) 21, 55, 140, 232, 266, 575, 653, 676, 813
Alw21I GWGCWC 1 cut(s) 23
AlwI GGATC 4 cut(s) 128, 274, 287, 694
Ama87I CYCGRG 1 cut(s) 16
AoxI GGCC 1 cut(s) 181
ApoI RAATTY 1 cut(s) 9
Asp700I GAANNNNTTC 1 cut(s) 54
AspS9I GGNCC 1 cut(s) 635
AsuHPI GGTGA 3 cut(s) 160, 401, 504
AvaI CYCGRG 1 cut(s) 16
AvaII GGWCC 1 cut(s) 635
BaeGI GKGCMC 1 cut(s) 419
BamHI GGATCC 1 cut(s) 279
BanI GGYRCC 1 cut(s) 414
BanII GRGCYC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 585
Bbv12I GWGCWC 1 cut(s) 23
BccI CCATC 3 cut(s) 139, 249, 884
BciVI GTATCC 1 cut(s) 212
BclI TGATCA 1 cut(s) 486
BfaI CTAG 3 cut(s) 27, 737, 783
BfmI CTRYAG 1 cut(s) 408
BfuI GTATCC 1 cut(s) 212
BisI GCNGC 1 cut(s) 333
BlpI GCTNAGC 1 cut(s) 814
BlsI GCNGC 1 cut(s) 334
Bme18I GGWCC 1 cut(s) 635
BmeT110I CYCGRG 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 635
BmiI GGNNCC 3 cut(s) 281, 416, 589
BpiI GAAGAC 1 cut(s) 585
Bpu1102I GCTNAGC 1 cut(s) 814
Bsa29I ATCGAT 1 cut(s) 298
BsaBI GATNNNNATC 1 cut(s) 240
BsaHI GRCGYC 1 cut(s) 763
BsaJI CCNNGG 2 cut(s) 184, 703
Bsc4I CCNNNNNNNGG 1 cut(s) 723
Bse118I RCCGGY 1 cut(s) 380
Bse1I ACTGG 1 cut(s) 333
Bse8I GATNNNNATC 1 cut(s) 240
BseCI ATCGAT 1 cut(s) 298
BseDI CCNNGG 2 cut(s) 184, 703
BseGI GGATG 4 cut(s) 241, 378, 616, 943
BseJI GATNNNNATC 1 cut(s) 240
BseLI CCNNNNNNNGG 1 cut(s) 723
BseMII CTCAG 2 cut(s) 271, 828
BseNI ACTGG 1 cut(s) 333
BseSI GKGCMC 1 cut(s) 419
BseYI CCCAGC 1 cut(s) 94
Bsh1236I CGCG 1 cut(s) 866
BshFI GGCC 1 cut(s) 183
BshNI GGYRCC 1 cut(s) 414
BshVI ATCGAT 1 cut(s) 298
BsiHKAI GWGCWC 1 cut(s) 23
BsiHKCI CYCGRG 1 cut(s) 16
BsiSI CCGG 1 cut(s) 381
BslFI GGGAC 1 cut(s) 884
BslI CCNNNNNNNGG 1 cut(s) 723
BsmFI GGGAC 1 cut(s) 884
BsmI GAATGC 2 cut(s) 315, 428
BsnI GGCC 1 cut(s) 183
BsoBI CYCGRG 1 cut(s) 16
Bsp1286I GDGCHC 2 cut(s) 23, 419
Bsp1720I GCTNAGC 1 cut(s) 814
Bsp19I CCATGG 1 cut(s) 184
BspACI CCGC 3 cut(s) 250, 332, 818
BspANI GGCC 1 cut(s) 183
BspCNI CTCAG 2 cut(s) 270, 827
BspDI ATCGAT 1 cut(s) 298
BspFNI CGCG 1 cut(s) 866
BspHI TCATGA 1 cut(s) 58
BspLI GGNNCC 3 cut(s) 281, 416, 589
BspPI GGATC 4 cut(s) 128, 274, 287, 694
BspT107I GGYRCC 1 cut(s) 414
BsrFI RCCGGY 1 cut(s) 380
BsrI ACTGG 1 cut(s) 333
BssAI RCCGGY 1 cut(s) 380
BssECI CCNNGG 2 cut(s) 184, 703
BssNI GRCGYC 1 cut(s) 763
BssT1I CCWWGG 2 cut(s) 184, 703
Bst4CI ACNGT 2 cut(s) 718, 852
Bst6I CTCTTC 1 cut(s) 312
BstACI GRCGYC 1 cut(s) 763
BstAPI GCANNNNNTGC 1 cut(s) 434
BstDEI CTNAG 3 cut(s) 257, 362, 814
BstDSI CCRYGG 1 cut(s) 184
BstF5I GGATG 4 cut(s) 241, 378, 616, 943
BstFNI CGCG 1 cut(s) 866
BstMWI GCNNNNNNNGC 4 cut(s) 52, 272, 434, 853
BstNSI RCATGY 1 cut(s) 991
BstSFI CTRYAG 1 cut(s) 408
BstSLI GKGCMC 1 cut(s) 419
BstUI CGCG 1 cut(s) 866
BstV2I GAAGAC 1 cut(s) 585
BstX2I RGATCY 2 cut(s) 279, 699
BstXI CCANNNNNNTGG 1 cut(s) 607
BstYI RGATCY 2 cut(s) 279, 699
Bsu15I ATCGAT 1 cut(s) 298
BsuI GTATCC 1 cut(s) 212
BsuRI GGCC 1 cut(s) 183
BsuTUI ATCGAT 1 cut(s) 298
BtgI CCRYGG 1 cut(s) 184
BtgZI GCGATG 1 cut(s) 903
BtsCI GGATG 4 cut(s) 241, 378, 616, 943
BtsI GCAGTG 1 cut(s) 842
BtsIMutI CAGTG 3 cut(s) 470, 842, 848
CciI TCATGA 1 cut(s) 58
Cfr10I RCCGGY 1 cut(s) 380
Cfr13I GGNCC 1 cut(s) 635
ClaI ATCGAT 1 cut(s) 298
CseI GACGC 1 cut(s) 752
Csp6I GTAC 1 cut(s) 714
CviAII CATG 7 cut(s) 59, 100, 169, 185, 505, 665, 988
CviQI GTAC 1 cut(s) 714
DdeI CTNAG 3 cut(s) 257, 362, 814
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 2 cut(s) 184, 703
Eco24I GRGCYC 1 cut(s) 23
Eco47I GGWCC 1 cut(s) 635
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 597
Eco88I CYCGRG 1 cut(s) 16
EcoICRI GAGCTC 1 cut(s) 21
EcoT14I CCWWGG 2 cut(s) 184, 703
EcoT38I GRGCYC 1 cut(s) 23
ErhI CCWWGG 2 cut(s) 184, 703
FaeI CATG 7 cut(s) 62, 103, 172, 188, 508, 668, 991
FaqI GGGAC 1 cut(s) 884
FatI CATG 7 cut(s) 58, 99, 168, 184, 504, 664, 987
FauNDI CATATG 1 cut(s) 602
FbaI TGATCA 1 cut(s) 486
Fnu4HI GCNGC 1 cut(s) 333
FokI GGATG 4 cut(s) 248, 385, 623, 950
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 1 cut(s) 333
FspBI CTAG 3 cut(s) 27, 737, 783
GluI GCNGC 1 cut(s) 333
GsaI CCCAGC 1 cut(s) 98
HaeIII GGCC 1 cut(s) 183
HapII CCGG 1 cut(s) 381
HgaI GACGC 1 cut(s) 752
Hin1I GRCGYC 1 cut(s) 763
Hin1II CATG 7 cut(s) 62, 103, 172, 188, 508, 668, 991
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 3 cut(s) 123, 151, 755
HpaII CCGG 1 cut(s) 381
HphI GGTGA 3 cut(s) 160, 401, 504
Hpy166II GTNNAC 1 cut(s) 541
Hpy188I TCNGA 5 cut(s) 260, 312, 552, 768, 925
Hpy188III TCNNGA 5 cut(s) 16, 59, 131, 349, 731
Hpy8I GTNNAC 1 cut(s) 541
HpyAV CCTTC 5 cut(s) 467, 557, 610, 697, 889
HpyCH4III ACNGT 2 cut(s) 718, 852
HpyCH4V TGCA 6 cut(s) 325, 428, 437, 468, 563, 683
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 272, 434, 853
HpyF3I CTNAG 3 cut(s) 257, 362, 814
Hsp92I GRCGYC 1 cut(s) 763
Hsp92II CATG 7 cut(s) 62, 103, 172, 188, 508, 668, 991
Ksp22I TGATCA 1 cut(s) 486
LmnI GCTCC 4 cut(s) 69, 312, 587, 673
MaeI CTAG 3 cut(s) 27, 737, 783
MaeIII GTNAC 4 cut(s) 339, 510, 620, 775
MboII GAAGA 4 cut(s) 299, 544, 590, 812
MflI RGATCY 2 cut(s) 279, 699
MhlI GDGCHC 2 cut(s) 23, 419
MluCI AATT 3 cut(s) 9, 858, 905
MlyI GAGTC 1 cut(s) 764
MmeI TCCRAC 1 cut(s) 427
MnlI CCTC 5 cut(s) 173, 266, 295, 445, 963
MroXI GAANNNNTTC 1 cut(s) 54
MseI TTAA 2 cut(s) 212, 722
MslI CAYNNNNRTG 3 cut(s) 320, 605, 794
MspA1I CMGCKG 1 cut(s) 818
MspI CCGG 1 cut(s) 381
Mva1269I GAATGC 2 cut(s) 315, 428
MvnI CGCG 1 cut(s) 866
MwoI GCNNNNNNNGC 4 cut(s) 52, 272, 434, 853
NcoI CCATGG 1 cut(s) 184
NdeI CATATG 1 cut(s) 602
NlaIII CATG 7 cut(s) 62, 103, 172, 188, 508, 668, 991
NlaIV GGNNCC 3 cut(s) 281, 416, 589
NmuCI GTSAC 3 cut(s) 339, 510, 775
NspI RCATGY 1 cut(s) 991
PaeR7I CTCGAG 1 cut(s) 16
PagI TCATGA 1 cut(s) 58
PciI ACATGT 1 cut(s) 987
PcsI WCGNNNNNNNCGW 2 cut(s) 870, 879
PctI GAATGC 2 cut(s) 315, 428
PdmI GAANNNNTTC 1 cut(s) 54
PfeI GAWTC 2 cut(s) 123, 151
PkrI GCNGC 1 cut(s) 334
PleI GAGTC 1 cut(s) 763
PpsI GAGTC 1 cut(s) 763
PscI ACATGT 1 cut(s) 987
PsiI TTATAA 1 cut(s) 659
Psp124BI GAGCTC 1 cut(s) 23
PspFI CCCAGC 1 cut(s) 94
PspN4I GGNNCC 3 cut(s) 281, 416, 589
PspPI GGNCC 1 cut(s) 635
PsuI RGATCY 2 cut(s) 279, 699
RsaI GTAC 1 cut(s) 715
RsaNI GTAC 1 cut(s) 714
RseI CAYNNNNRTG 3 cut(s) 320, 605, 794
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 212, 722
SatI GCNGC 1 cut(s) 333
Sau96I GGNCC 1 cut(s) 635
SchI GAGTC 1 cut(s) 764
SduI GDGCHC 2 cut(s) 23, 419
SfcI CTRYAG 1 cut(s) 408
Sfr274I CTCGAG 1 cut(s) 16
SinI GGWCC 1 cut(s) 635
SlaI CTCGAG 1 cut(s) 16
SmiMI CAYNNNNRTG 3 cut(s) 320, 605, 794
SmlI CTYRAG 1 cut(s) 16
SmoI CTYRAG 1 cut(s) 16
Sse9I AATT 3 cut(s) 9, 858, 905
SsiI CCGC 3 cut(s) 250, 332, 818
SspMI CTAG 3 cut(s) 27, 737, 783
SstI GAGCTC 1 cut(s) 23
StyI CCWWGG 2 cut(s) 184, 703
TaaI ACNGT 2 cut(s) 718, 852
TaqI TCGA 3 cut(s) 17, 178, 298
TasI AATT 3 cut(s) 9, 858, 905
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 2 cut(s) 123, 151
Tru1I TTAA 2 cut(s) 212, 722
Tru9I TTAA 2 cut(s) 212, 722
TscAI CASTG 3 cut(s) 470, 849, 855
TseFI GTSAC 3 cut(s) 339, 510, 775
Tsp45I GTSAC 3 cut(s) 339, 510, 775
TspDTI ATGAA 5 cut(s) 17, 47, 164, 521, 545
TspRI CASTG 3 cut(s) 470, 849, 855
VpaK11BI GGWCC 1 cut(s) 635
XapI RAATTY 1 cut(s) 9
XceI RCATGY 1 cut(s) 991
XcmI CCANNNNNNNNNTGG 1 cut(s) 864
XhoI CTCGAG 1 cut(s) 16
XmnI GAANNNNTTC 1 cut(s) 54
XspI CTAG 3 cut(s) 27, 737, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.