Rw2G033450
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002891
Physical Location & Seq
Forward (+)
54771028 .. 54771675
648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033450.1

Sequence Viewer

Length: 648 bp
ATGGCAATGCAGATCAAGAAGCCGAGCCAGGGTCGCCAAAAGATTGCAATCACCAAAATTCCCAACAGGAGTAATTTACAAGTCACATTCTCGAAACGTTGTGCAGGACTGTTCAAGAAGGCTAGTGAGCTCTGCACACTTTGTGGAGTTGAGATTGCAATAGTAGTCTTTTCTCCTTCCAACAAACCCTTCTCTTTCGGCCATCCTAGCATTAAGACCCTCATCGGCAGCTTTCTTTCTCCAAGCCCTTCATCATTTCCCAGTAGTACTCAACAAGCTCTGTACCCCAGGAAGGCTAGTGTTGAACATGAGCTCAACATGCAGCTAACCGGGACTCAAGGCCGACTGGAGGGTGAGAAAAAGCGAGGAGAAGAGCTTGACAAGATGAGTAAAGACAGCCAGCGATCGAGTCATTGTTGGTGGGAGAAGCCAATTGAGGAACTTGGATTGGATGGGCTCCAGATGTTGAAGGCTGCAATGGAGGAGCTCAAGAAGAATGTGATGGAACAAATCCACAAAATAAATGGTCCTGCCAAATTTTCAGTACCATTCTCTGTGATGAATCTTGATCATCATCTTTCTGAGAGAAAGTTTGCTGAGATGAATGAATGTCATGGTTCCATGATCCCTTCTTGTGCCTATGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04013 ko04022 ko04371 ko04921 ko05202 ko05418 map04010 map04013 map04022 map04371 map04921 map05202 map05418
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

215

Amino Acids

23.98

Weight (kDa)

9.22

Isoelectric Point (pI)

58.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 19 - 66 1.4e-24 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 97
AclWI GGATC 1 cut(s) 619
AcoI YGGCCR 1 cut(s) 199
AcsI RAATTY 2 cut(s) 57, 536
AdeI CACNNNGTG 1 cut(s) 143
AfaI GTAC 3 cut(s) 268, 284, 546
AfiI CCNNNNNNNGG 3 cut(s) 29, 292, 349
AgsI TTSAA 3 cut(s) 115, 305, 469
AjnI CCWGG 2 cut(s) 27, 287
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AluBI AGCT 7 cut(s) 130, 231, 278, 313, 325, 376, 487
AluI AGCT 7 cut(s) 130, 231, 278, 313, 325, 376, 487
Alw21I GWGCWC 3 cut(s) 132, 315, 489
AlwI GGATC 1 cut(s) 619
AoxI GGCC 2 cut(s) 199, 340
ApeKI GCWGC 3 cut(s) 228, 322, 473
ApoI RAATTY 2 cut(s) 57, 536
AspS9I GGNCC 1 cut(s) 527
AsuC2I CCSGG 1 cut(s) 331
AsuHPI GGTGA 2 cut(s) 43, 365
AvaII GGWCC 1 cut(s) 527
BanII GRGCYC 4 cut(s) 132, 315, 459, 489
Bbv12I GWGCWC 3 cut(s) 132, 315, 489
BbvI GCAGC 3 cut(s) 240, 334, 460
BccI CCATC 3 cut(s) 210, 446, 496
BciT130I CCWGG 2 cut(s) 29, 289
BclI TGATCA 1 cut(s) 568
BcnI CCSGG 1 cut(s) 331
BfaI CTAG 4 cut(s) 123, 207, 297, 646
BisI GCNGC 3 cut(s) 229, 323, 474
BlsI GCNGC 3 cut(s) 230, 324, 475
BmcAI AGTACT 1 cut(s) 268
Bme1390I CCNGG 3 cut(s) 29, 289, 331
Bme18I GGWCC 1 cut(s) 527
BmgT120I GGNCC 1 cut(s) 527
BmiI GGNNCC 2 cut(s) 458, 619
BmrFI CCNGG 3 cut(s) 29, 289, 331
BmrI ACTGGG 1 cut(s) 255
BmuI ACTGGG 1 cut(s) 255
BpmI CTGGAG 2 cut(s) 368, 443
BpuEI CTTGAG 2 cut(s) 321, 473
BpuMI CCSGG 1 cut(s) 331
BsaBI GATNNNNATC 2 cut(s) 47, 573
BsaJI CCNNGG 2 cut(s) 28, 287
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 292, 349
Bse1I ACTGG 2 cut(s) 261, 351
Bse3DI GCAATG 2 cut(s) 12, 483
Bse8I GATNNNNATC 2 cut(s) 47, 573
BseBI CCWGG 2 cut(s) 29, 289
BseDI CCNNGG 2 cut(s) 28, 287
BseGI GGATG 2 cut(s) 202, 457
BseJI GATNNNNATC 2 cut(s) 47, 573
BseLI CCNNNNNNNGG 3 cut(s) 29, 292, 349
BseMI GCAATG 2 cut(s) 12, 483
BseMII CTCAG 2 cut(s) 573, 588
BseNI ACTGG 2 cut(s) 261, 351
BseRI GAGGAG 2 cut(s) 381, 497
BseXI GCAGC 3 cut(s) 240, 334, 460
BsgI GTGCAG 2 cut(s) 118, 123
Bsh1285I CGRYCG 1 cut(s) 407
BshFI GGCC 2 cut(s) 201, 342
BsiEI CGRYCG 1 cut(s) 407
BsiHKAI GWGCWC 3 cut(s) 132, 315, 489
BsiSI CCGG 1 cut(s) 330
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 3 cut(s) 29, 292, 349
BsmFI GGGAC 1 cut(s) 346
BsnI GGCC 2 cut(s) 201, 342
Bsp1286I GDGCHC 4 cut(s) 132, 315, 459, 489
Bsp143I GATC 4 cut(s) 12, 404, 568, 624
BspANI GGCC 2 cut(s) 201, 342
BspCNI CTCAG 2 cut(s) 574, 589
BspLI GGNNCC 2 cut(s) 458, 619
BspPI GGATC 1 cut(s) 619
BspQI GCTCTTC 1 cut(s) 366
BsrDI GCAATG 2 cut(s) 12, 483
BsrI ACTGG 2 cut(s) 261, 351
BssECI CCNNGG 2 cut(s) 28, 287
BssMI GATC 4 cut(s) 12, 404, 568, 624
Bst2UI CCWGG 2 cut(s) 29, 289
Bst4CI ACNGT 1 cut(s) 111
Bst6I CTCTTC 1 cut(s) 366
BstC8I GCNNGC 1 cut(s) 401
BstDEI CTNAG 2 cut(s) 582, 597
BstF5I GGATG 2 cut(s) 202, 457
BstKTI GATC 4 cut(s) 15, 407, 571, 627
BstMBI GATC 4 cut(s) 12, 404, 568, 624
BstMCI CGRYCG 1 cut(s) 407
BstMWI GCNNNNNNNGC 3 cut(s) 33, 207, 319
BstNI CCWGG 2 cut(s) 29, 289
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 3 cut(s) 27, 287, 329
BstV1I GCAGC 3 cut(s) 240, 334, 460
BsuRI GGCC 2 cut(s) 201, 342
BtsCI GGATG 2 cut(s) 202, 457
Cac8I GCNNGC 1 cut(s) 401
Cfr13I GGNCC 1 cut(s) 527
Csp6I GTAC 3 cut(s) 267, 283, 545
CviAII CATG 4 cut(s) 308, 319, 614, 622
CviQI GTAC 3 cut(s) 267, 283, 545
DdeI CTNAG 2 cut(s) 582, 597
DpnI GATC 4 cut(s) 14, 406, 570, 626
DpnII GATC 4 cut(s) 12, 404, 568, 624
DraIII CACNNNGTG 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
Ecl136II GAGCTC 3 cut(s) 130, 313, 487
Eco24I GRGCYC 4 cut(s) 132, 315, 459, 489
Eco47I GGWCC 1 cut(s) 527
Eco53kI GAGCTC 3 cut(s) 130, 313, 487
EcoICRI GAGCTC 3 cut(s) 130, 313, 487
EcoRII CCWGG 2 cut(s) 27, 287
EcoT38I GRGCYC 4 cut(s) 132, 315, 459, 489
FaeI CATG 4 cut(s) 311, 322, 617, 625
FaiI YATR 5 cut(s) 309, 320, 615, 623, 642
FaqI GGGAC 1 cut(s) 346
FatI CATG 4 cut(s) 307, 318, 613, 621
FbaI TGATCA 1 cut(s) 568
Fnu4HI GCNGC 3 cut(s) 229, 323, 474
FokI GGATG 2 cut(s) 189, 464
FriOI GRGCYC 4 cut(s) 132, 315, 459, 489
Fsp4HI GCNGC 3 cut(s) 229, 323, 474
FspBI CTAG 4 cut(s) 123, 207, 297, 646
GluI GCNGC 3 cut(s) 229, 323, 474
GsuI CTGGAG 2 cut(s) 368, 443
HaeIII GGCC 2 cut(s) 201, 342
HapII CCGG 1 cut(s) 330
Hin1II CATG 4 cut(s) 311, 322, 617, 625
HinfI GANTC 3 cut(s) 334, 409, 562
HpaII CCGG 1 cut(s) 330
HphI GGTGA 2 cut(s) 43, 365
Hpy188I TCNGA 1 cut(s) 583
Hpy188III TCNNGA 6 cut(s) 16, 91, 115, 460, 490, 566
HpyAV CCTTC 7 cut(s) 112, 186, 199, 258, 286, 463, 639
HpyCH4III ACNGT 1 cut(s) 111
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 7 cut(s) 10, 47, 104, 135, 158, 322, 476
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 207, 319
HpyF3I CTNAG 2 cut(s) 582, 597
HpySE526I ACGT 1 cut(s) 97
Hsp92II CATG 4 cut(s) 311, 322, 617, 625
Ksp22I TGATCA 1 cut(s) 568
Kzo9I GATC 4 cut(s) 12, 404, 568, 624
LguI GCTCTTC 1 cut(s) 366
LmnI GCTCC 2 cut(s) 462, 484
Lsp1109I GCAGC 3 cut(s) 240, 334, 460
MaeI CTAG 4 cut(s) 123, 207, 297, 646
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 1 cut(s) 82
MalI GATC 4 cut(s) 14, 406, 570, 626
MboI GATC 4 cut(s) 12, 404, 568, 624
MboII GAAGA 2 cut(s) 383, 505
MfeI CAATTG 1 cut(s) 432
MhlI GDGCHC 4 cut(s) 132, 315, 459, 489
MluCI AATT 4 cut(s) 57, 73, 432, 536
MlyI GAGTC 2 cut(s) 328, 418
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 5 cut(s) 230, 343, 359, 430, 475
MseI TTAA 1 cut(s) 213
MspI CCGG 1 cut(s) 330
MspR9I CCNGG 3 cut(s) 29, 289, 331
MunI CAATTG 1 cut(s) 432
MvaI CCWGG 2 cut(s) 29, 289
MwoI GCNNNNNNNGC 3 cut(s) 33, 207, 319
NciI CCSGG 1 cut(s) 331
NdeII GATC 4 cut(s) 12, 404, 568, 624
NlaIII CATG 4 cut(s) 311, 322, 617, 625
NlaIV GGNNCC 2 cut(s) 458, 619
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 82
NspI RCATGY 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 562
PkrI GCNGC 3 cut(s) 230, 324, 475
Ple19I CGATCG 1 cut(s) 407
PleI GAGTC 2 cut(s) 328, 417
PpsI GAGTC 2 cut(s) 328, 417
Psp124BI GAGCTC 3 cut(s) 132, 315, 489
Psp1406I AACGTT 1 cut(s) 97
Psp6I CCWGG 2 cut(s) 27, 287
PspGI CCWGG 2 cut(s) 27, 287
PspN4I GGNNCC 2 cut(s) 458, 619
PspPI GGNCC 1 cut(s) 527
PvuI CGATCG 1 cut(s) 407
RsaI GTAC 3 cut(s) 268, 284, 546
RsaNI GTAC 3 cut(s) 267, 283, 545
SacI GAGCTC 3 cut(s) 132, 315, 489
SapI GCTCTTC 1 cut(s) 366
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 3 cut(s) 229, 323, 474
Sau3AI GATC 4 cut(s) 12, 404, 568, 624
Sau96I GGNCC 1 cut(s) 527
ScaI AGTACT 1 cut(s) 268
SchI GAGTC 2 cut(s) 328, 418
ScrFI CCNGG 3 cut(s) 29, 289, 331
SduI GDGCHC 4 cut(s) 132, 315, 459, 489
SetI ASST 8 cut(s) 100, 132, 233, 280, 315, 327, 378, 489
SinI GGWCC 1 cut(s) 527
SmlI CTYRAG 2 cut(s) 336, 488
SmoI CTYRAG 2 cut(s) 336, 488
Sse9I AATT 4 cut(s) 57, 73, 432, 536
SspMI CTAG 4 cut(s) 123, 207, 297, 646
SstI GAGCTC 3 cut(s) 132, 315, 489
StyD4I CCNGG 3 cut(s) 27, 287, 329
TaaI ACNGT 1 cut(s) 111
TaiI ACGT 1 cut(s) 100
TaqI TCGA 2 cut(s) 92, 407
TasI AATT 4 cut(s) 57, 73, 432, 536
TatI WGTACW 1 cut(s) 266
TfiI GAWTC 1 cut(s) 562
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 3 cut(s) 228, 322, 473
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 4 cut(s) 240, 575, 617, 621
VpaK11BI GGWCC 1 cut(s) 527
XapI RAATTY 2 cut(s) 57, 536
XceI RCATGY 1 cut(s) 322
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
XspI CTAG 4 cut(s) 123, 207, 297, 646
ZrmI AGTACT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.