Rw5G038170

acyl-CoA hydrolase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004837
Physical Location & Seq
Forward (+)
67441914 .. 67444169
2256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G038170.1

Sequence Viewer

Length: 2151 bp
ATGCTTTTTGAAACAATAGAAGAAGCCAGAAAGTATTATGAAGATTATGGTCGTCAAGAAGGCTTTTGGATTCAAACTCAATCTTCAAGTAAGACAAGATCACATTCGGACGAGGTGACTAGTTGTCATTTCGTTTGTTCACATCAGGGAAAATATATCTGGAAGAAAAAGATTAATGAGGAGTGGAAAAAAAACCAGCAGAGAGAGACTTGTGACAATGAGAATGAAATTGGAGATGAGAATGGGAATGAGAATGTTGATAAAGGCAGAGATATGAATATAAAGAAAGTAGGTAAAAATTGTTCTACACCGAAATGTGGGTGCAAAGCTAGTATGCGAGTCATTCATGAGAAGTGGTCGAACAAATGGAGAGTATCTGTTTTTAGGGACTTTCACAATCATAAACTAGTTACTCCAGCAAGAAGAATGAAGATGAAGTCAAATAAACATATGCCTAAAGCAGTGAGGAGTATGACTGAAATATTCCATAAAGAAAATTTGCCGATTGCAAAAGTTCATTCAATCCTTGGTGGTGCTCAAATTGGTTTTAACAAAAGAGATTGTTATAACCACTTAAGAAATGTTCGTCATAGACAATTGGATGGAGGAGATGCTCAGTCAGTCCTCACTTACTTCAGGAAAAAGCAAGCTGAGAATCCCCAATTTTTTTATGCAATCCAATGCAACGAAGATGGAAGAGCAACTAATTTCTTTTGGGTCGACTCTCGATCATGGACAGCTTATGAATATTTTGGAGATGTGGTCACATTCGACACAACTTATAGGACAAACAAGTATGATATGCCGTTTGCGCCATTCACAGGAGTAAACCACCACTGGCAATCAATACAATTCGGCTATGCACTTCTACAAGATGAGACGGAGGCTACCTTTTTTTGGTTATTTGAGACATGGCTAGAAGCAATGGGAGGTCGTCATCCAATTTCCATTATTACTGACCAAGACTTGGCAATGAAAGGAGCAATTGCCAAGATATTTCCAAATACTCGCCATCGTTTATGCCTTTGGCATATCAAGAAGAAGTTTGTTGAGAAGTTGTCCCATGTGTACTATAAGAAATCGAAATTCAAGAAGGAGATGAAGAAATACTTTGATGACAGAAATGGATTGGAAGAAAATGAATGGTTAAATAATCTTTATGAGATACGTACTTCTTGGGTGCCGGTTTATCATCGTGGAACATTTTTTGCAGGAATGAATACTATTGGACATAGTGAGGGAATTAATTCATTTTTCGATGGATTTGTTACTTCAACAACAAATCTTAGAGAATTTGTTGTTAAATATGATCAAGCACTAAAGAGGATTATAGATATTGAAAGTGATGAAGACTTTGAAACAGAACACAAGTTTCGTATCGTGAATGATGATGAATTCCTTTTGAAAGATGCAGCAAAGTTGTATACCAGAAATGTTTTCAACAAATTCAAGGTACAATGGAGTCAAATTAAGCACTACAAGCAAGATTTTATGGTGAAGTTAGATTTGACAACATACAAGGGTTCTTGTGAGTGCCAAAACTTTGAGTTTGTTGGAATACTTTGTCGGCACTTGTTAAAAGTATTTTCACGTTTGGATATTGAAACAATACCAGCACATTTTATTCTTCCAAGATGGAGACAAGAAGCAAACAAATTTAGGATAATGGATTCTGAAGATTTGGTGAAAAGTGTTGGTAAAGAAGAATCAGAAGCTGTAAGACTTAGCCATATGTGTCACCAAGCAACTAAATTGGCCTTTATTGCTGCACCTTCAAAGGAGGGATACACAATCTTCATGGATGCTTTAAAGGACGTATCAAGAAAATTGCCAAAAATTTCAGATCAGCCTGCTATCATTAGGGAAATGAGTGTTCCTCTAAACCAAGATGATCATACTACCATGGAAACTAGTGCTTCCCCAAATAGAGATGATCCATGTACTAATATTCTTTCTTCCCAGTCATTGCTTTTGGACCCAAATATCTCTAAGACCAAAGGTAGGAAGAAAGAAACTAAGAGTTCTGGCAGAATAAAGAGTGGTGTTGAGTTGGCCATGGAGACAGGGAAAAGAAGGTGTACATTATGCTCCAAGTCAGGACACAACAAACAAACTTGTTCTTTGAATTCACAAAGAAGAAAGATTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

716

Amino Acids

83.84

Weight (kDa)

9.17

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 95 - 137 7.7e-06 FAR1 DNA-binding domain
ZSWIM1-3_RNaseH-like PF21056 220 - 337 5.8e-08 Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE PF10551 254 - 348 1.4e-31 MULE transposase domain
SWIM PF04434 507 - 532 4e-06 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 567
AccB1I GGYRCC 1 cut(s) 1180
AccB7I CCANNNNNTGG 1 cut(s) 967
AccI GTMKAC 2 cut(s) 720, 1424
AclWI GGATC 1 cut(s) 1928
AcoI YGGCCR 1 cut(s) 2052
AcsI RAATTY 8 cut(s) 496, 1085, 1292, 1394, 1445, 1655, 1836, 2125
AcuI CTGAAG 2 cut(s) 619, 1695
AfaI GTAC 5 cut(s) 1070, 1171, 1455, 1942, 2080
AfiI CCNNNNNNNGG 5 cut(s) 317, 821, 897, 967, 2001
AflII CTTAAG 1 cut(s) 574
AhdI GACNNNNNGTC 1 cut(s) 123
AhlI ACTAGT 3 cut(s) 119, 406, 1910
AluBI AGCT 4 cut(s) 329, 650, 740, 1715
AluI AGCT 4 cut(s) 329, 650, 740, 1715
Alw21I GWGCWC 1 cut(s) 538
Alw26I GTCTC 5 cut(s) 200, 872, 902, 1633, 2054
AlwI GGATC 1 cut(s) 1928
AlwNI CAGNNNCTG 1 cut(s) 1715
AoxI GGCC 2 cut(s) 1755, 2052
ApeKI GCWGC 2 cut(s) 1412, 1766
ApoI RAATTY 8 cut(s) 496, 1085, 1292, 1394, 1445, 1655, 1836, 2125
AseI ATTAAT 2 cut(s) 174, 1245
Asp700I GAANNNNTTC 1 cut(s) 1246
AspLEI GCGC 1 cut(s) 814
AspS9I GGNCC 1 cut(s) 1975
AsuHPI GGTGA 4 cut(s) 127, 1507, 1696, 1730
AvaII GGWCC 1 cut(s) 1975
BalI TGGCCA 1 cut(s) 2054
BanI GGYRCC 1 cut(s) 1180
BbsI GAAGAC 1 cut(s) 1356
Bbv12I GWGCWC 1 cut(s) 538
BbvI GCAGC 2 cut(s) 1424, 1753
BccI CCATC 5 cut(s) 596, 686, 1020, 1253, 1629
BceAI ACGGC 1 cut(s) 790
BciVI GTATCC 1 cut(s) 1778
BclI TGATCA 2 cut(s) 1309, 1891
BcoDI GTCTC 5 cut(s) 200, 872, 902, 1633, 2054
BcuI ACTAGT 3 cut(s) 119, 406, 1910
BfaI CTAG 5 cut(s) 120, 330, 407, 917, 1911
BfrI CTTAAG 1 cut(s) 574
BfuI GTATCC 1 cut(s) 1778
BisI GCNGC 2 cut(s) 1413, 1767
BlsI GCNGC 2 cut(s) 1414, 1768
Bme18I GGWCC 1 cut(s) 1975
BmeRI GACNNNNNGTC 1 cut(s) 123
BmgT120I GGNCC 1 cut(s) 1975
BmiI GGNNCC 2 cut(s) 1182, 1977
BmrI ACTGGG 1 cut(s) 1954
BmsI GCATC 3 cut(s) 601, 1399, 1792
BmuI ACTGGG 1 cut(s) 1954
BpiI GAAGAC 1 cut(s) 1356
BplI GAGNNNNNCTC 2 cut(s) 1861, 1893
BpmI CTGGAG 1 cut(s) 399
BsaAI YACGTR 1 cut(s) 1169
BsaJI CCNNGG 3 cut(s) 526, 1902, 2055
BsaXI ACNNNNNCTCC 2 cut(s) 747, 777
Bsc4I CCNNNNNNNGG 5 cut(s) 317, 821, 897, 967, 2001
Bse118I RCCGGY 1 cut(s) 1183
Bse1I ACTGG 2 cut(s) 842, 1960
Bse3DI GCAATG 3 cut(s) 930, 978, 1964
BseDI CCNNGG 3 cut(s) 526, 1902, 2055
BseGI GGATG 3 cut(s) 607, 937, 1807
BseLI CCNNNNNNNGG 5 cut(s) 317, 821, 897, 967, 2001
BseMI GCAATG 3 cut(s) 930, 978, 1964
BseMII CTCAG 2 cut(s) 629, 642
BseNI ACTGG 2 cut(s) 842, 1960
BseRI GAGGAG 3 cut(s) 194, 481, 621
BseXI GCAGC 2 cut(s) 1424, 1753
BsgI GTGCAG 1 cut(s) 1752
BshFI GGCC 2 cut(s) 1757, 2054
BshNI GGYRCC 1 cut(s) 1180
BsiHKAI GWGCWC 1 cut(s) 538
BsiSI CCGG 1 cut(s) 1184
BslFI GGGAC 2 cut(s) 401, 1045
BslI CCNNNNNNNGG 5 cut(s) 317, 821, 897, 967, 2001
BsmAI GTCTC 5 cut(s) 200, 872, 902, 1633, 2054
BsmBI CGTCTC 1 cut(s) 872
BsmFI GGGAC 2 cut(s) 401, 1045
BsnI GGCC 2 cut(s) 1757, 2054
Bsp1286I GDGCHC 1 cut(s) 538
Bsp1407I TGTACA 1 cut(s) 2078
Bsp143I GATC 6 cut(s) 98, 728, 1309, 1843, 1891, 1933
Bsp19I CCATGG 2 cut(s) 1902, 2055
BspANI GGCC 2 cut(s) 1757, 2054
BspCNI CTCAG 2 cut(s) 628, 643
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 2 cut(s) 1182, 1977
BspPI GGATC 1 cut(s) 1928
BspQI GCTCTTC 1 cut(s) 691
BspT107I GGYRCC 1 cut(s) 1180
BspTI CTTAAG 1 cut(s) 574
BsrDI GCAATG 3 cut(s) 930, 978, 1964
BsrFI RCCGGY 1 cut(s) 1183
BsrGI TGTACA 1 cut(s) 2078
BsrI ACTGG 2 cut(s) 842, 1960
BssAI RCCGGY 1 cut(s) 1183
BssECI CCNNGG 3 cut(s) 526, 1902, 2055
BssMI GATC 6 cut(s) 98, 728, 1309, 1843, 1891, 1933
BssNAI GTATAC 1 cut(s) 1425
BssT1I CCWWGG 3 cut(s) 526, 1902, 2055
Bst1107I GTATAC 1 cut(s) 1425
Bst6I CTCTTC 1 cut(s) 691
BstAFI CTTAAG 1 cut(s) 574
BstAUI TGTACA 1 cut(s) 2078
BstBAI YACGTR 1 cut(s) 1169
BstC8I GCNNGC 2 cut(s) 648, 1851
BstDEI CTNAG 6 cut(s) 615, 651, 1286, 1724, 1989, 2016
BstDSI CCRYGG 2 cut(s) 1902, 2055
BstF5I GGATG 3 cut(s) 607, 937, 1807
BstHHI GCGC 1 cut(s) 814
BstKTI GATC 6 cut(s) 101, 731, 1312, 1846, 1894, 1936
BstMAI GTCTC 5 cut(s) 200, 872, 902, 1633, 2054
BstMBI GATC 6 cut(s) 98, 728, 1309, 1843, 1891, 1933
BstMWI GCNNNNNNNGC 3 cut(s) 811, 1480, 1763
BstSNI TACGTA 1 cut(s) 1169
BstV1I GCAGC 2 cut(s) 1424, 1753
BstV2I GAAGAC 1 cut(s) 1356
BstZ17I GTATAC 1 cut(s) 1425
BsuI GTATCC 1 cut(s) 1778
BsuRI GGCC 2 cut(s) 1757, 2054
BtgI CCRYGG 2 cut(s) 1902, 2055
BtsCI GGATG 3 cut(s) 607, 937, 1807
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 2 cut(s) 468, 835
Cac8I GCNNGC 2 cut(s) 648, 1851
CaiI CAGNNNCTG 1 cut(s) 1715
CciI TCATGA 1 cut(s) 346
CfoI GCGC 1 cut(s) 814
Cfr10I RCCGGY 1 cut(s) 1183
Cfr13I GGNCC 1 cut(s) 1975
Csp6I GTAC 5 cut(s) 1069, 1170, 1454, 1941, 2079
CviAII CATG 8 cut(s) 347, 732, 912, 1064, 1798, 1903, 1938, 2056
CviQI GTAC 5 cut(s) 1069, 1170, 1454, 1941, 2079
DdeI CTNAG 6 cut(s) 615, 651, 1286, 1724, 1989, 2016
DpnI GATC 6 cut(s) 100, 730, 1311, 1845, 1893, 1935
DpnII GATC 6 cut(s) 98, 728, 1309, 1843, 1891, 1933
DraI TTTAAA 1 cut(s) 1809
DriI GACNNNNNGTC 1 cut(s) 123
EaeI YGGCCR 1 cut(s) 2052
Eam1104I CTCTTC 1 cut(s) 691
Eam1105I GACNNNNNGTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 691
Eco105I TACGTA 1 cut(s) 1169
Eco130I CCWWGG 3 cut(s) 526, 1902, 2055
Eco47I GGWCC 1 cut(s) 1975
Eco57I CTGAAG 2 cut(s) 619, 1695
EcoRI GAATTC 2 cut(s) 1394, 2125
EcoT14I CCWWGG 3 cut(s) 526, 1902, 2055
ErhI CCWWGG 3 cut(s) 526, 1902, 2055
Esp3I CGTCTC 1 cut(s) 872
FaeI CATG 8 cut(s) 350, 735, 915, 1067, 1801, 1906, 1941, 2059
FalI AAGNNNNNCTT 2 cut(s) 1094, 1126
FaqI GGGAC 2 cut(s) 401, 1045
FatI CATG 8 cut(s) 346, 731, 911, 1063, 1797, 1902, 1937, 2055
FauNDI CATATG 2 cut(s) 450, 1731
FbaI TGATCA 2 cut(s) 1309, 1891
FblI GTMKAC 2 cut(s) 720, 1424
Fnu4HI GCNGC 2 cut(s) 1413, 1767
FokI GGATG 3 cut(s) 614, 924, 1814
Fsp4HI GCNGC 2 cut(s) 1413, 1767
FspBI CTAG 5 cut(s) 120, 330, 407, 917, 1911
GlaI GCGC 1 cut(s) 813
GluI GCNGC 2 cut(s) 1413, 1767
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 2 cut(s) 1757, 2054
HapII CCGG 1 cut(s) 1184
HhaI GCGC 1 cut(s) 814
Hin1II CATG 8 cut(s) 350, 735, 915, 1067, 1801, 1906, 1941, 2059
Hin6I GCGC 1 cut(s) 812
HinP1I GCGC 1 cut(s) 812
HincII GTYRAC 1 cut(s) 721
HindII GTYRAC 1 cut(s) 721
HinfI GANTC 8 cut(s) 70, 339, 655, 722, 1462, 1670, 1706, 2143
HpaII CCGG 1 cut(s) 1184
HphI GGTGA 4 cut(s) 127, 1507, 1696, 1730
Hpy166II GTNNAC 6 cut(s) 140, 721, 829, 1069, 1425, 2079
Hpy188I TCNGA 4 cut(s) 109, 1675, 1711, 1843
Hpy8I GTNNAC 6 cut(s) 140, 721, 829, 1069, 1425, 2079
HpyAV CCTTC 4 cut(s) 53, 1087, 1782, 2067
HpyCH4IV ACGT 3 cut(s) 1168, 1591, 1815
HpyCH4V TGCA 8 cut(s) 324, 509, 674, 684, 863, 1211, 1412, 1769
HpyF10VI GCNNNNNNNGC 3 cut(s) 811, 1480, 1763
HpyF3I CTNAG 6 cut(s) 615, 651, 1286, 1724, 1989, 2016
HpySE526I ACGT 3 cut(s) 1168, 1591, 1815
Hsp92II CATG 8 cut(s) 350, 735, 915, 1067, 1801, 1906, 1941, 2059
HspAI GCGC 1 cut(s) 812
Ksp22I TGATCA 2 cut(s) 1309, 1891
Kzo9I GATC 6 cut(s) 98, 728, 1309, 1843, 1891, 1933
LguI GCTCTTC 1 cut(s) 691
LmnI GCTCC 2 cut(s) 980, 2093
Lsp1109I GCAGC 2 cut(s) 1424, 1753
LweI GCATC 3 cut(s) 601, 1399, 1792
MaeI CTAG 5 cut(s) 120, 330, 407, 917, 1911
MaeII ACGT 3 cut(s) 1168, 1591, 1815
MaeIII GTNAC 6 cut(s) 115, 212, 409, 763, 1267, 1736
MalI GATC 6 cut(s) 100, 730, 1311, 1845, 1893, 1935
MboI GATC 6 cut(s) 98, 728, 1309, 1843, 1891, 1933
MfeI CAATTG 2 cut(s) 596, 984
MhlI GDGCHC 1 cut(s) 538
MlsI TGGCCA 1 cut(s) 2054
MluNI TGGCCA 1 cut(s) 2054
MlyI GAGTC 3 cut(s) 348, 716, 1471
MmeI TCCRAC 1 cut(s) 1534
Mox20I TGGCCA 1 cut(s) 2054
MroXI GAANNNNTTC 1 cut(s) 1246
MscI TGGCCA 1 cut(s) 2054
MseI TTAA 9 cut(s) 174, 549, 575, 1148, 1245, 1302, 1470, 1577, 1808
MslI CAYNNNNRTG 1 cut(s) 313
Msp20I TGGCCA 1 cut(s) 2054
MspCI CTTAAG 1 cut(s) 574
MspI CCGG 1 cut(s) 1184
MunI CAATTG 2 cut(s) 596, 984
MwoI GCNNNNNNNGC 3 cut(s) 811, 1480, 1763
NcoI CCATGG 2 cut(s) 1902, 2055
NdeI CATATG 2 cut(s) 450, 1731
NdeII GATC 6 cut(s) 98, 728, 1309, 1843, 1891, 1933
NlaIII CATG 8 cut(s) 350, 735, 915, 1067, 1801, 1906, 1941, 2059
NlaIV GGNNCC 2 cut(s) 1182, 1977
NmuCI GTSAC 4 cut(s) 115, 212, 763, 1736
PagI TCATGA 1 cut(s) 346
PciSI GCTCTTC 1 cut(s) 691
PdmI GAANNNNTTC 1 cut(s) 1246
PfeI GAWTC 5 cut(s) 70, 655, 1670, 1706, 2143
PflMI CCANNNNNTGG 1 cut(s) 967
PkrI GCNGC 2 cut(s) 1414, 1768
PleI GAGTC 3 cut(s) 347, 716, 1470
PpsI GAGTC 3 cut(s) 347, 716, 1470
Ppu21I YACGTR 1 cut(s) 1169
PshBI ATTAAT 2 cut(s) 174, 1245
PsiI TTATAA 1 cut(s) 567
PspN4I GGNNCC 2 cut(s) 1182, 1977
PspPI GGNCC 1 cut(s) 1975
PsrI GAACNNNNNNTAC 2 cut(s) 286, 318
PstNI CAGNNNCTG 1 cut(s) 1715
RsaI GTAC 5 cut(s) 1070, 1171, 1455, 1942, 2080
RsaNI GTAC 5 cut(s) 1069, 1170, 1454, 1941, 2079
RseI CAYNNNNRTG 1 cut(s) 313
SalI GTCGAC 1 cut(s) 719
SapI GCTCTTC 1 cut(s) 691
SaqAI TTAA 9 cut(s) 174, 549, 575, 1148, 1245, 1302, 1470, 1577, 1808
SatI GCNGC 2 cut(s) 1413, 1767
Sau3AI GATC 6 cut(s) 98, 728, 1309, 1843, 1891, 1933
Sau96I GGNCC 1 cut(s) 1975
SchI GAGTC 3 cut(s) 348, 716, 1471
SduI GDGCHC 1 cut(s) 538
SfaNI GCATC 3 cut(s) 601, 1399, 1792
SinI GGWCC 1 cut(s) 1975
SmiMI CAYNNNNRTG 1 cut(s) 313
SmlI CTYRAG 1 cut(s) 574
SmoI CTYRAG 1 cut(s) 574
SnaBI TACGTA 1 cut(s) 1169
SpeI ACTAGT 3 cut(s) 119, 406, 1910
SspI AATATT 3 cut(s) 483, 749, 1948
SspMI CTAG 5 cut(s) 120, 330, 407, 917, 1911
StyI CCWWGG 3 cut(s) 526, 1902, 2055
TaiI ACGT 3 cut(s) 1171, 1594, 1818
TaqI TCGA 6 cut(s) 359, 720, 727, 771, 1082, 1257
TatI WGTACW 3 cut(s) 1068, 1940, 2078
TfiI GAWTC 5 cut(s) 70, 655, 1670, 1706, 2143
Tru1I TTAA 9 cut(s) 174, 549, 575, 1148, 1245, 1302, 1470, 1577, 1808
Tru9I TTAA 9 cut(s) 174, 549, 575, 1148, 1245, 1302, 1470, 1577, 1808
TscAI CASTG 2 cut(s) 468, 842
TseFI GTSAC 4 cut(s) 115, 212, 763, 1736
TseI GCWGC 2 cut(s) 1412, 1766
Tsp45I GTSAC 4 cut(s) 115, 212, 763, 1736
TspGWI ACGGA 1 cut(s) 896
TspRI CASTG 2 cut(s) 468, 842
Van91I CCANNNNNTGG 1 cut(s) 967
Vha464I CTTAAG 1 cut(s) 574
VpaK11BI GGWCC 1 cut(s) 1975
VspI ATTAAT 2 cut(s) 174, 1245
XapI RAATTY 8 cut(s) 496, 1085, 1292, 1394, 1445, 1655, 1836, 2125
XmiI GTMKAC 2 cut(s) 720, 1424
XmnI GAANNNNTTC 1 cut(s) 1246
XspI CTAG 5 cut(s) 120, 330, 407, 917, 1911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.