AT1G03280

General transcription factor IIE subunit 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001364
Physical Location & Seq
Forward (+)
802970 .. 807066
4097 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G03280.2

Sequence Viewer

Length: 1440 bp
ATGGAAAAATCAGGCCCGGTGCAGAAAGCCGTTGTTCTCCAGCCATTTGTCAAGTTGGTGAGGCTTGTAGCGAGGGCTTTCTATGATGATTATACTACGAAAAGCGATAATCAGCAGAAATCTGCTAGAAGTGACAACAGAGGGATTGCCGCTGTGGTGCTAGACGCGCTGGCAAGGCGGCAATGGGTTAGAGAAGAAGACTTGGCAAAAGATTTGCAGTTGCACGCCAAGCAACTTCGAAAAATAATAAGACTCTTTGAAGAAGAAAAGTTGATTATGCGTGACCACCGGAAAGAGACTGCAAAGGGTGCAAAGATGTATAGTGCTGCAGTGGCTGCTACAACTGATGGCCGAGCAGAGGACAAAGTTAAGCTTCACACCCACTCGTATTGCTGTCTCGATTATGCACAGATATGTGACGTTGTTCGTTTTAGACTGCATCGGATGAAAAAAAGGCTCAAAGATGAGCTAGAAGATAAGAATACTGTTCAGGAGTATGGCTGTCCTAACTGCCAAAGGAAATATAATGCGCTGGATGCTCTGAGATTAATTTCTATGGTAGACGATTCTTTTCATTGTGAAAATTGCAATGGTGAACTTGTTGTGGAATGCAACAAGCTAACTTCCGAAGAAGTAGTTGATGGAGATGATAATGCAAGGAGACGGCGGCGGGAAAACCTAAAAAATATGCTTCAGAAATTGGAGGTTCAAATGAAACCTCTGATGGATCAGTTAAATAGAGTAAAAGACCTACCAATACCTGAATTTGGATCTTTTCTGGCATGGGAGGCCCGTGCAGCGATGGCTGCTCGCGAGAATGGTGATCTTAACCCTAATGATCCTTTGAGGTCACAGGGCGGGTATGGTTCAACACCAATGCCATTTCTCGGAGAGACAAAGGTTGAGGTTAACCTAGGTGATGGAAACGAAGATGTTAAATCTAAAGGTGGAGACTCCAGTCTGAAAGTCTTGCCTCCATGGATGATCAAGGAAGGAATGAATCTGACCGAGGAACAAAGAGGAGAGATGAGACAGGAAGCAAAGGTTGATGGTGGGGCAGGAGCAGCAGCAAAACTTTCAGATGACAAGAAATCAGCCATTGGAAATGGAGATGAGAAGGATTTGAAGGATGAGTATCTCAAAGCTTACTACGCCGAACTTATGAAGCAGCAAGAGCTTGCTGCAAGGCGAAATCAACAAGAATCCGCTGGTGAACCAACTTCTGGCATTCAGTCGGGTACTGTATACTCAGGTCGTCAGGTTAGTATGAAGGCCAAACGTGAAGAGGATGAAGACGAAGATGAAGAAGAAGTAGAATGGGAAGAAAAGGCTCCTGTTACTGCTAATGGAAACTATAAGGTGGACTTGAATGTGGAAGCAGAAGCTTCGGGTGGTGAAGAAGAAGAAGAGGAAGACGACGTCGATTGGGAAGAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

479

Amino Acids

54.12

Weight (kDa)

5.02

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIE_alpha PF02002 50 - 107 5.8e-07 TFIIE alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1422
AccB7I CCANNNNNTGG 1 cut(s) 1223
AccI GTMKAC 2 cut(s) 561, 1245
AccII CGCG 2 cut(s) 167, 813
AciI CCGC 6 cut(s) 150, 178, 667, 670, 858, 1206
AclWI GGATC 3 cut(s) 735, 778, 833
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 1 cut(s) 764
AcuI CTGAAG 1 cut(s) 677
AcyI GRCGYC 1 cut(s) 1419
AfaI GTAC 1 cut(s) 1240
AfiI CCNNNNNNNGG 4 cut(s) 358, 767, 887, 1223
AgsI TTSAA 5 cut(s) 260, 710, 870, 1126, 1369
AluBI AGCT 6 cut(s) 373, 469, 619, 1145, 1177, 1385
AluI AGCT 6 cut(s) 373, 469, 619, 1145, 1177, 1385
Alw26I GTCTC 6 cut(s) 290, 401, 655, 887, 945, 1024
AlwI GGATC 3 cut(s) 735, 778, 833
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 4 cut(s) 13, 349, 789, 1272
ApeKI GCWGC 8 cut(s) 326, 335, 797, 806, 1064, 1067, 1168, 1181
ApoI RAATTY 1 cut(s) 764
AseI ATTAAT 1 cut(s) 548
AspA2I CCTAGG 1 cut(s) 913
AspLEI GCGC 2 cut(s) 169, 532
AspS9I GGNCC 2 cut(s) 14, 790
AsuC2I CCSGG 1 cut(s) 17
AsuHPI GGTGA 6 cut(s) 70, 605, 833, 929, 1223, 1406
AsuII TTCGAA 1 cut(s) 238
AvrII CCTAGG 1 cut(s) 913
BbsI GAAGAC 3 cut(s) 204, 1299, 1419
BbvI GCAGC 8 cut(s) 313, 322, 793, 809, 1076, 1079, 1168, 1180
BccI CCATC 6 cut(s) 341, 635, 718, 796, 914, 1043
BceAI ACGGC 2 cut(s) 14, 680
BcgI CGANNNNNNTGC 2 cut(s) 1368, 1402
BclI TGATCA 1 cut(s) 984
BcnI CCSGG 1 cut(s) 17
BcoDI GTCTC 6 cut(s) 290, 401, 655, 887, 945, 1024
BfaI CTAG 4 cut(s) 126, 161, 470, 914
BfmI CTRYAG 1 cut(s) 327
BlnI CCTAGG 1 cut(s) 913
Bme1390I CCNGG 1 cut(s) 17
BmgT120I GGNCC 2 cut(s) 14, 790
BmiI GGNNCC 1 cut(s) 1332
BmrFI CCNGG 1 cut(s) 17
BmsI GCATC 2 cut(s) 448, 526
BoxI GACNNNNGTC 1 cut(s) 957
BpiI GAAGAC 3 cut(s) 204, 1299, 1419
BpmI CTGGAG 2 cut(s) 23, 940
Bpu14I TTCGAA 1 cut(s) 238
BpuMI CCSGG 1 cut(s) 17
BsaBI GATNNNNATC 1 cut(s) 1134
BsaHI GRCGYC 1 cut(s) 1419
BsaJI CCNNGG 3 cut(s) 913, 977, 1008
BsaWI WCCGGW 1 cut(s) 288
Bsc4I CCNNNNNNNGG 4 cut(s) 358, 767, 887, 1223
Bse1I ACTGG 1 cut(s) 957
Bse3DI GCAATG 2 cut(s) 188, 595
Bse8I GATNNNNATC 1 cut(s) 1134
BseDI CCNNGG 3 cut(s) 913, 977, 1008
BseGI GGATG 5 cut(s) 450, 541, 987, 1135, 1294
BseJI GATNNNNATC 1 cut(s) 1134
BseLI CCNNNNNNNGG 4 cut(s) 358, 767, 887, 1223
BseMI GCAATG 2 cut(s) 188, 595
BseMII CTCAG 2 cut(s) 533, 1263
BseNI ACTGG 1 cut(s) 957
BseRI GAGGAG 1 cut(s) 1035
BseXI GCAGC 8 cut(s) 313, 322, 793, 809, 1076, 1079, 1168, 1180
BsgI GTGCAG 2 cut(s) 41, 816
Bsh1236I CGCG 2 cut(s) 167, 813
BshFI GGCC 4 cut(s) 15, 351, 791, 1274
BsiSI CCGG 2 cut(s) 17, 289
BslI CCNNNNNNNGG 4 cut(s) 358, 767, 887, 1223
BsmAI GTCTC 6 cut(s) 290, 401, 655, 887, 945, 1024
BsmBI CGTCTC 1 cut(s) 655
BsmI GAATGC 2 cut(s) 614, 1227
BsnI GGCC 4 cut(s) 15, 351, 791, 1274
Bsp119I TTCGAA 1 cut(s) 238
Bsp143I GATC 5 cut(s) 727, 770, 823, 838, 984
Bsp19I CCATGG 1 cut(s) 977
Bsp68I TCGCGA 1 cut(s) 813
BspACI CCGC 6 cut(s) 150, 178, 667, 670, 858, 1206
BspANI GGCC 4 cut(s) 15, 351, 791, 1274
BspCNI CTCAG 2 cut(s) 534, 1262
BspFNI CGCG 2 cut(s) 167, 813
BspLI GGNNCC 1 cut(s) 1332
BspMAI CTGCAG 1 cut(s) 331
BspPI GGATC 3 cut(s) 735, 778, 833
BspT104I TTCGAA 1 cut(s) 238
BsrDI GCAATG 2 cut(s) 188, 595
BsrI ACTGG 1 cut(s) 957
BssECI CCNNGG 3 cut(s) 913, 977, 1008
BssMI GATC 5 cut(s) 727, 770, 823, 838, 984
BssNAI GTATAC 1 cut(s) 1246
BssNI GRCGYC 1 cut(s) 1419
BssT1I CCWWGG 2 cut(s) 913, 977
Bst1107I GTATAC 1 cut(s) 1246
Bst4CI ACNGT 2 cut(s) 487, 1243
Bst6I CTCTTC 2 cut(s) 1278, 1401
BstACI GRCGYC 1 cut(s) 1419
BstAPI GCANNNNNTGC 2 cut(s) 308, 335
BstBI TTCGAA 1 cut(s) 238
BstC8I GCNNGC 4 cut(s) 171, 225, 811, 1179
BstDEI CTNAG 2 cut(s) 542, 1249
BstDSI CCRYGG 1 cut(s) 977
BstF5I GGATG 5 cut(s) 450, 541, 987, 1135, 1294
BstFNI CGCG 2 cut(s) 167, 813
BstHHI GCGC 2 cut(s) 169, 532
BstKTI GATC 5 cut(s) 730, 773, 826, 841, 987
BstMAI GTCTC 6 cut(s) 290, 401, 655, 887, 945, 1024
BstMBI GATC 5 cut(s) 727, 770, 823, 838, 984
BstPAI GACNNNNGTC 1 cut(s) 957
BstSCI CCNGG 1 cut(s) 15
BstSFI CTRYAG 1 cut(s) 327
BstUI CGCG 2 cut(s) 167, 813
BstV1I GCAGC 8 cut(s) 313, 322, 793, 809, 1076, 1079, 1168, 1180
BstV2I GAAGAC 3 cut(s) 204, 1299, 1419
BstX2I RGATCY 1 cut(s) 770
BstYI RGATCY 1 cut(s) 770
BstZ17I GTATAC 1 cut(s) 1246
BsuRI GGCC 4 cut(s) 15, 351, 791, 1274
BtgI CCRYGG 1 cut(s) 977
BtgZI GCGATG 1 cut(s) 815
BtsCI GGATG 5 cut(s) 450, 541, 987, 1135, 1294
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 1 cut(s) 336
BtuMI TCGCGA 1 cut(s) 813
Cac8I GCNNGC 4 cut(s) 171, 225, 811, 1179
CaiI CAGNNNCTG 1 cut(s) 335
CfoI GCGC 2 cut(s) 169, 532
Cfr13I GGNCC 2 cut(s) 14, 790
CseI GACGC 1 cut(s) 173
Csp6I GTAC 1 cut(s) 1239
CspCI CAANNNNNGTGG 2 cut(s) 371, 406
CviAII CATG 2 cut(s) 783, 978
CviQI GTAC 1 cut(s) 1239
DdeI CTNAG 2 cut(s) 542, 1249
DpnI GATC 5 cut(s) 729, 772, 825, 840, 986
DpnII GATC 5 cut(s) 727, 770, 823, 838, 984
EaeI YGGCCR 1 cut(s) 349
Eam1104I CTCTTC 2 cut(s) 1278, 1401
EarI CTCTTC 2 cut(s) 1278, 1401
Eco130I CCWWGG 2 cut(s) 913, 977
Eco57I CTGAAG 1 cut(s) 677
EcoT14I CCWWGG 2 cut(s) 913, 977
ErhI CCWWGG 2 cut(s) 913, 977
Esp3I CGTCTC 1 cut(s) 655
FaeI CATG 2 cut(s) 786, 981
FalI AAGNNNNNCTT 4 cut(s) 357, 389, 1349, 1381
FatI CATG 2 cut(s) 782, 977
FauI CCCGC 2 cut(s) 663, 851
FbaI TGATCA 1 cut(s) 984
FblI GTMKAC 2 cut(s) 561, 1245
FokI GGATG 5 cut(s) 457, 548, 994, 1142, 1301
FspBI CTAG 4 cut(s) 126, 161, 470, 914
GlaI GCGC 2 cut(s) 168, 531
GsuI CTGGAG 2 cut(s) 23, 940
HaeIII GGCC 4 cut(s) 15, 351, 791, 1274
HapII CCGG 2 cut(s) 17, 289
HgaI GACGC 1 cut(s) 173
HhaI GCGC 2 cut(s) 169, 532
Hin1I GRCGYC 1 cut(s) 1419
Hin1II CATG 2 cut(s) 786, 981
Hin6I GCGC 2 cut(s) 167, 530
HinP1I GCGC 2 cut(s) 167, 530
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HindIII AAGCTT 3 cut(s) 371, 1143, 1383
HinfI GANTC 5 cut(s) 252, 566, 953, 1000, 1202
HpaI GTTAAC 1 cut(s) 910
HpaII CCGG 2 cut(s) 17, 289
HphI GGTGA 6 cut(s) 70, 605, 833, 929, 1223, 1406
Hpy166II GTNNAC 6 cut(s) 562, 596, 910, 1214, 1246, 1363
Hpy188I TCNGA 9 cut(s) 444, 543, 628, 696, 723, 890, 963, 1005, 1081
Hpy188III TCNNGA 3 cut(s) 398, 491, 812
Hpy8I GTNNAC 6 cut(s) 562, 596, 910, 1214, 1246, 1363
Hpy99I CGWCG 2 cut(s) 1421, 1424
HpyAV CCTTC 5 cut(s) 986, 1111, 1120, 1264, 1427
HpyCH4III ACNGT 2 cut(s) 487, 1243
HpyCH4IV ACGT 3 cut(s) 420, 1279, 1419
HpyF3I CTNAG 2 cut(s) 542, 1249
HpySE526I ACGT 3 cut(s) 420, 1279, 1419
Hsp92I GRCGYC 1 cut(s) 1419
Hsp92II CATG 2 cut(s) 786, 981
HspAI GCGC 2 cut(s) 167, 530
Ksp22I TGATCA 1 cut(s) 984
KspAI GTTAAC 1 cut(s) 910
Kzo9I GATC 5 cut(s) 727, 770, 823, 838, 984
LmnI GCTCC 2 cut(s) 1061, 1336
Lsp1109I GCAGC 8 cut(s) 313, 322, 793, 809, 1076, 1079, 1168, 1180
LweI GCATC 2 cut(s) 448, 526
MaeI CTAG 4 cut(s) 126, 161, 470, 914
MaeII ACGT 3 cut(s) 420, 1279, 1419
MaeIII GTNAC 5 cut(s) 131, 281, 416, 849, 1336
MalI GATC 5 cut(s) 729, 772, 825, 840, 986
MboI GATC 5 cut(s) 727, 770, 823, 838, 984
MflI RGATCY 1 cut(s) 770
MluCI AATT 4 cut(s) 549, 583, 698, 764
MlyI GAGTC 2 cut(s) 246, 947
MseI TTAA 6 cut(s) 369, 548, 734, 828, 909, 936
MslI CAYNNNNRTG 1 cut(s) 412
MspA1I CMGCKG 2 cut(s) 152, 1208
MspI CCGG 2 cut(s) 17, 289
MspR9I CCNGG 1 cut(s) 17
Mva1269I GAATGC 2 cut(s) 614, 1227
MvnI CGCG 2 cut(s) 167, 813
NciI CCSGG 1 cut(s) 17
NcoI CCATGG 1 cut(s) 977
NdeII GATC 5 cut(s) 727, 770, 823, 838, 984
NlaIII CATG 2 cut(s) 786, 981
NlaIV GGNNCC 1 cut(s) 1332
NmeAIII GCCGAG 1 cut(s) 377
NmuCI GTSAC 4 cut(s) 131, 281, 416, 849
NruI TCGCGA 1 cut(s) 813
NspV TTCGAA 1 cut(s) 238
PctI GAATGC 2 cut(s) 614, 1227
PfeI GAWTC 3 cut(s) 566, 1000, 1202
PflFI GACNNNGTC 1 cut(s) 1418
PflMI CCANNNNNTGG 1 cut(s) 1223
PleI GAGTC 2 cut(s) 246, 947
PpsI GAGTC 2 cut(s) 246, 947
PshAI GACNNNNGTC 1 cut(s) 957
PshBI ATTAAT 1 cut(s) 548
PspN4I GGNNCC 1 cut(s) 1332
PspPI GGNCC 2 cut(s) 14, 790
PstI CTGCAG 1 cut(s) 331
PstNI CAGNNNCTG 1 cut(s) 335
PsuI RGATCY 1 cut(s) 770
PsyI GACNNNGTC 1 cut(s) 1418
RruI TCGCGA 1 cut(s) 813
RsaI GTAC 1 cut(s) 1240
RsaNI GTAC 1 cut(s) 1239
RseI CAYNNNNRTG 1 cut(s) 412
SaqAI TTAA 6 cut(s) 369, 548, 734, 828, 909, 936
Sau3AI GATC 5 cut(s) 727, 770, 823, 838, 984
Sau96I GGNCC 2 cut(s) 14, 790
SchI GAGTC 2 cut(s) 246, 947
ScrFI CCNGG 1 cut(s) 17
SfaNI GCATC 2 cut(s) 448, 526
SfcI CTRYAG 1 cut(s) 327
SfuI TTCGAA 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 412
Sse9I AATT 4 cut(s) 549, 583, 698, 764
SsiI CCGC 6 cut(s) 150, 178, 667, 670, 858, 1206
SspMI CTAG 4 cut(s) 126, 161, 470, 914
StyD4I CCNGG 1 cut(s) 15
StyI CCWWGG 2 cut(s) 913, 977
TaaI ACNGT 2 cut(s) 487, 1243
TaiI ACGT 3 cut(s) 423, 1282, 1422
TaqI TCGA 3 cut(s) 238, 399, 1422
TaqII GACCGA 1 cut(s) 1022
TasI AATT 4 cut(s) 549, 583, 698, 764
TauI GCSGC 3 cut(s) 152, 181, 670
TfiI GAWTC 3 cut(s) 566, 1000, 1202
Tru1I TTAA 6 cut(s) 369, 548, 734, 828, 909, 936
Tru9I TTAA 6 cut(s) 369, 548, 734, 828, 909, 936
TscAI CASTG 1 cut(s) 336
TseFI GTSAC 4 cut(s) 131, 281, 416, 849
TseI GCWGC 8 cut(s) 326, 335, 797, 806, 1064, 1067, 1168, 1181
Tsp45I GTSAC 4 cut(s) 131, 281, 416, 849
TspDTI ATGAA 8 cut(s) 461, 563, 728, 1013, 1178, 1283, 1305, 1317
TspRI CASTG 1 cut(s) 336
Tth111I GACNNNGTC 1 cut(s) 1418
Van91I CCANNNNNTGG 1 cut(s) 1223
VspI ATTAAT 1 cut(s) 548
XapI RAATTY 1 cut(s) 764
XmaJI CCTAGG 1 cut(s) 913
XmiI GTMKAC 2 cut(s) 561, 1245
XspI CTAG 4 cut(s) 126, 161, 470, 914
ZraI GACGTC 1 cut(s) 1420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.