Rw7G005600

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001364
Physical Location & Seq
Reverse (-)
4707658 .. 4711825
4168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G005600.1

Sequence Viewer

Length: 1320 bp
ATGAGTATCGAGCCTTTCAATAGGTTGGTGAAGCTCGCGGCGAGGGCGTTCTACGATGACATAACCACGAAAGGGGATAACCAGCCCAAGACTGGCCGGAGCGACAATCGGGGAATTGCCGTGGTTGTGCTCGACGCGCTCTCAAGGCGACAATGGGTGAGAGAAGAGGATTTGGCAAAGGACTTGAAACTGCACTCGAAGCAACTTCGTCGTACTTTGCGATTTTTTGAAGGGAGAAACTGTGCTGCAGTTGCGGCAACAGCTGACGGGCAACAACATACAAAAGAAGGGGAAGAGAAAATTAAGTTGCACACTCACTCTTACTGCTGTCTGGACTATGCTCAGATATATGATGTAGTAAGGTACAGACTGCACCGTATGAGGAAAAAACTGAAAGATGAATTGGAGGACAAGAACACAGTTCAGGAATATGTATGCCCTAACTGTGGGAAAAGATACAATGCTTTGGATGCACTGCAACTCATCTCTTTGGAGGATGAGTACTTCCACTGCGAGAGTTGCAATGGGGAGCTTGTGGCTGAGAGTGACAAGCTAGCTTCTCAAGATGTAGGAGATGGAGATAGCAATGCAAGGAGGCAAAGGCGTGAAAAACTGAAAGACATGCTTCAAAATATGGAGGTACAGCTGAAGCCTTTAATGGAACAAATTAATAGGGTGAAAGACTTACCTGCTCCTGAATTTGGAACTCTCCAAGCTTGGGAAGCACGAGCACATGCTCAACGACGTGCAGAGAATGGAGAATCTAGTGCCATGGATCCCTCCAAATCTTCACATGGGCTAGGGTACGGTGGAACGCCCATGCCTTTTCTTGGAGATACAAAAGTTGAAGTTGATTTTGGGAATGAAGGCAAGGGTAACGATGTCAAATCAGAAGCTGATATTACAGGCCAGAAAATTTTGCCACCTTGGATGATAAAGCAAGGGATGAACCTGACAAAGGAACAGCGTGGAGAGGTAAAGCAGGAGGCAAAGATGGATGGCACGTTAGCAGCTCCAGAGTATTCAGATGACAAAAAGTCAAATGCTGAAGATAATGATAAGGCTTTCCAGGATGAGTATTTCAAAGCCTATTACGCTGCTTTAATAAAAAGACAAGAAGAAGAAGCAGCAAAGAGTCGTCAGGTTGGCATGAAGTCAAAACGTGATCTGAATGAAGGAGATGAAGGAGAAGATAATGTTGACTGGCAAGAGGCTCCAGTTGCAGGTGATGCCAATGGAAATTACAAGGTCAATGACTTAAATGTCGAAGCCGATGCTACTGCAGAGGATGAAGATGACATAGACTGGGAGGAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

439

Amino Acids

49.7

Weight (kDa)

5.04

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIE_alpha PF02002 40 - 138 1.7e-09 TFIIE alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1214
AasI GACNNNNNNGTC 1 cut(s) 1262
Acc36I ACCTGC 2 cut(s) 697, 1214
AccII CGCG 2 cut(s) 38, 137
AciI CCGC 2 cut(s) 38, 254
AclWI GGATC 2 cut(s) 770, 783
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 2 cut(s) 698, 915
AcuI CTGAAG 2 cut(s) 668, 1068
AfaI GTAC 5 cut(s) 214, 365, 503, 642, 806
AfiI CCNNNNNNNGG 8 cut(s) 72, 92, 446, 701, 718, 830, 958, 1223
AgsI TTSAA 6 cut(s) 19, 187, 230, 629, 848, 1084
AhdI GACNNNNNGTC 1 cut(s) 1036
AjiI CACGTC 1 cut(s) 746
AjnI CCWGG 1 cut(s) 1068
AjuI GAANNNNNNNTTGG 4 cut(s) 386, 418, 840, 872
AluBI AGCT 9 cut(s) 34, 263, 532, 553, 557, 646, 716, 896, 1013
AluI AGCT 9 cut(s) 34, 263, 532, 553, 557, 646, 716, 896, 1013
Alw21I GWGCWC 2 cut(s) 132, 733
AlwI GGATC 2 cut(s) 770, 783
AlwNI CAGNNNCTG 1 cut(s) 896
AoxI GGCC 2 cut(s) 94, 907
ApeKI GCWGC 4 cut(s) 245, 1010, 1097, 1127
ApoI RAATTY 2 cut(s) 698, 915
AseI ATTAAT 1 cut(s) 669
AspLEI GCGC 1 cut(s) 139
AsuHPI GGTGA 4 cut(s) 40, 169, 688, 1238
AsuNHI GCTAGC 1 cut(s) 553
BaeI ACNNNNGTAYC 2 cut(s) 828, 861
BamHI GGATCC 1 cut(s) 775
BauI CACGAG 1 cut(s) 726
Bbv12I GWGCWC 2 cut(s) 132, 733
BbvI GCAGC 4 cut(s) 232, 1022, 1084, 1139
BccI CCATC 3 cut(s) 569, 988, 992
BceAI ACGGC 1 cut(s) 104
BcgI CGANNNNNNTGC 6 cut(s) 191, 225, 1256, 1262, 1290, 1296
BciT130I CCWGG 1 cut(s) 1070
BfaI CTAG 3 cut(s) 554, 765, 800
BfmI CTRYAG 2 cut(s) 246, 1281
BfuAI ACCTGC 2 cut(s) 697, 1214
BisI GCNGC 6 cut(s) 39, 246, 255, 1011, 1098, 1128
BlsI GCNGC 6 cut(s) 40, 247, 256, 1012, 1099, 1129
BmcAI AGTACT 1 cut(s) 503
Bme1390I CCNGG 1 cut(s) 1070
BmeRI GACNNNNNGTC 1 cut(s) 1036
BmgBI CACGTC 1 cut(s) 746
BmiI GGNNCC 2 cut(s) 777, 1215
BmrFI CCNGG 1 cut(s) 1070
BmrI ACTGGG 1 cut(s) 1315
BmsI GCATC 3 cut(s) 460, 1219, 1264
BmtI GCTAGC 1 cut(s) 557
BmuI ACTGGG 1 cut(s) 1315
BpmI CTGGAG 2 cut(s) 999, 1200
BpuEI CTTGAG 2 cut(s) 127, 546
BsaJI CCNNGG 3 cut(s) 120, 771, 926
BsaXI ACNNNNNCTCC 4 cut(s) 226, 256, 485, 515
Bsc4I CCNNNNNNNGG 8 cut(s) 72, 92, 446, 701, 718, 830, 958, 1223
Bse1I ACTGG 4 cut(s) 97, 1208, 1217, 1310
Bse3DI GCAATG 2 cut(s) 529, 592
BseBI CCWGG 1 cut(s) 1070
BseDI CCNNGG 3 cut(s) 120, 771, 926
BseGI GGATG 7 cut(s) 475, 502, 936, 951, 1003, 1078, 1294
BseLI CCNNNNNNNGG 8 cut(s) 72, 92, 446, 701, 718, 830, 958, 1223
BseMI GCAATG 2 cut(s) 529, 592
BseMII CTCAG 2 cut(s) 356, 531
BseNI ACTGG 4 cut(s) 97, 1208, 1217, 1310
BseXI GCAGC 4 cut(s) 232, 1022, 1084, 1139
BsgI GTGCAG 3 cut(s) 176, 356, 768
Bsh1236I CGCG 2 cut(s) 38, 137
BshFI GGCC 2 cut(s) 96, 909
BsiHKAI GWGCWC 2 cut(s) 132, 733
BsiSI CCGG 1 cut(s) 97
BslI CCNNNNNNNGG 8 cut(s) 72, 92, 446, 701, 718, 830, 958, 1223
BsnI GGCC 2 cut(s) 96, 909
Bsp1286I GDGCHC 2 cut(s) 132, 733
Bsp143I GATC 2 cut(s) 775, 1165
Bsp19I CCATGG 1 cut(s) 771
BspACI CCGC 2 cut(s) 38, 254
BspANI GGCC 2 cut(s) 96, 909
BspCNI CTCAG 2 cut(s) 355, 532
BspFNI CGCG 2 cut(s) 38, 137
BspLI GGNNCC 2 cut(s) 777, 1215
BspMAI CTGCAG 2 cut(s) 250, 1285
BspMI ACCTGC 2 cut(s) 697, 1214
BspOI GCTAGC 1 cut(s) 557
BspPI GGATC 2 cut(s) 770, 783
BsrDI GCAATG 2 cut(s) 529, 592
BsrI ACTGG 4 cut(s) 97, 1208, 1217, 1310
BssECI CCNNGG 3 cut(s) 120, 771, 926
BssMI GATC 2 cut(s) 775, 1165
BssSI CACGAG 1 cut(s) 726
BssT1I CCWWGG 2 cut(s) 771, 926
Bst2BI CACGAG 1 cut(s) 726
Bst2UI CCWGG 1 cut(s) 1070
Bst4CI ACNGT 5 cut(s) 242, 377, 421, 446, 809
Bst6I CTCTTC 2 cut(s) 159, 288
BstAPI GCANNNNNTGC 1 cut(s) 1229
BstC8I GCNNGC 2 cut(s) 36, 555
BstDEI CTNAG 2 cut(s) 342, 540
BstDSI CCRYGG 2 cut(s) 120, 771
BstENI CCTNNNNNAGG 1 cut(s) 956
BstF5I GGATG 7 cut(s) 475, 502, 936, 951, 1003, 1078, 1294
BstFNI CGCG 2 cut(s) 38, 137
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 2 cut(s) 778, 1168
BstMBI GATC 2 cut(s) 775, 1165
BstNI CCWGG 1 cut(s) 1070
BstNSI RCATGY 2 cut(s) 625, 737
BstSCI CCNGG 1 cut(s) 1068
BstSFI CTRYAG 2 cut(s) 246, 1281
BstUI CGCG 2 cut(s) 38, 137
BstV1I GCAGC 4 cut(s) 232, 1022, 1084, 1139
BstX2I RGATCY 1 cut(s) 775
BstYI RGATCY 1 cut(s) 775
BsuRI GGCC 2 cut(s) 96, 909
BtgI CCRYGG 2 cut(s) 120, 771
BtrI CACGTC 1 cut(s) 746
BtsCI GGATG 7 cut(s) 475, 502, 936, 951, 1003, 1078, 1294
BtsI GCAGTG 2 cut(s) 473, 508
BtsIMutI CAGTG 2 cut(s) 473, 508
BveI ACCTGC 2 cut(s) 697, 1214
Cac8I GCNNGC 2 cut(s) 36, 555
CaiI CAGNNNCTG 1 cut(s) 896
CfoI GCGC 1 cut(s) 139
CseI GACGC 1 cut(s) 143
Csp6I GTAC 5 cut(s) 213, 364, 502, 641, 805
CviAII CATG 6 cut(s) 622, 734, 772, 794, 820, 1150
CviQI GTAC 5 cut(s) 213, 364, 502, 641, 805
DdeI CTNAG 2 cut(s) 342, 540
DpnI GATC 2 cut(s) 777, 1167
DpnII GATC 2 cut(s) 775, 1165
DrdI GACNNNNNNGTC 1 cut(s) 1262
DriI GACNNNNNGTC 1 cut(s) 1036
DseDI GACNNNNNNGTC 1 cut(s) 1262
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 2 cut(s) 159, 288
Eam1105I GACNNNNNGTC 1 cut(s) 1036
EarI CTCTTC 2 cut(s) 159, 288
Eco130I CCWWGG 2 cut(s) 771, 926
Eco57I CTGAAG 2 cut(s) 668, 1068
EcoNI CCTNNNNNAGG 1 cut(s) 956
EcoRII CCWGG 1 cut(s) 1068
EcoT14I CCWWGG 2 cut(s) 771, 926
ErhI CCWWGG 2 cut(s) 771, 926
FaeI CATG 6 cut(s) 625, 737, 775, 797, 823, 1153
FalI AAGNNNNNCTT 2 cut(s) 609, 641
FatI CATG 6 cut(s) 621, 733, 771, 793, 819, 1149
Fnu4HI GCNGC 6 cut(s) 39, 246, 255, 1011, 1098, 1128
FokI GGATG 7 cut(s) 482, 509, 943, 958, 1010, 1085, 1301
Fsp4HI GCNGC 6 cut(s) 39, 246, 255, 1011, 1098, 1128
FspBI CTAG 3 cut(s) 554, 765, 800
GlaI GCGC 1 cut(s) 138
GluI GCNGC 6 cut(s) 39, 246, 255, 1011, 1098, 1128
GsuI CTGGAG 2 cut(s) 999, 1200
HaeIII GGCC 2 cut(s) 96, 909
HapII CCGG 1 cut(s) 97
HgaI GACGC 1 cut(s) 143
HhaI GCGC 1 cut(s) 139
Hin1II CATG 6 cut(s) 625, 737, 775, 797, 823, 1153
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HincII GTYRAC 1 cut(s) 1201
HindII GTYRAC 1 cut(s) 1201
HindIII AAGCTT 1 cut(s) 714
HinfI GANTC 2 cut(s) 761, 1135
HpaII CCGG 1 cut(s) 97
HphI GGTGA 4 cut(s) 40, 169, 688, 1238
Hpy166II GTNNAC 1 cut(s) 1201
Hpy188I TCNGA 4 cut(s) 345, 892, 1027, 1170
Hpy188III TCNNGA 5 cut(s) 332, 425, 563, 695, 1016
Hpy8I GTNNAC 1 cut(s) 1201
Hpy99I CGWCG 3 cut(s) 137, 213, 747
HpyAV CCTTC 5 cut(s) 224, 281, 860, 1169, 1178
HpyCH4III ACNGT 5 cut(s) 242, 377, 421, 446, 809
HpyCH4IV ACGT 3 cut(s) 745, 1004, 1162
HpyF3I CTNAG 2 cut(s) 342, 540
HpySE526I ACGT 3 cut(s) 745, 1004, 1162
Hsp92II CATG 6 cut(s) 625, 737, 775, 797, 823, 1153
HspAI GCGC 1 cut(s) 137
Kzo9I GATC 2 cut(s) 775, 1165
LmnI GCTCC 5 cut(s) 99, 529, 697, 1018, 1219
Lsp1109I GCAGC 4 cut(s) 232, 1022, 1084, 1139
LweI GCATC 3 cut(s) 460, 1219, 1264
MaeI CTAG 3 cut(s) 554, 765, 800
MaeII ACGT 3 cut(s) 745, 1004, 1162
MaeIII GTNAC 2 cut(s) 545, 875
MalI GATC 2 cut(s) 777, 1167
MboI GATC 2 cut(s) 775, 1165
MboII GAAGA 8 cut(s) 176, 305, 780, 1061, 1130, 1133, 1202, 1304
MflI RGATCY 1 cut(s) 775
MhlI GDGCHC 2 cut(s) 132, 733
MluCI AATT 7 cut(s) 114, 300, 401, 666, 698, 915, 1240
MlyI GAGTC 1 cut(s) 1144
MseI TTAA 5 cut(s) 303, 656, 669, 1103, 1259
MspA1I CMGCKG 2 cut(s) 263, 646
MspI CCGG 1 cut(s) 97
MspR9I CCNGG 1 cut(s) 1070
MvaI CCWGG 1 cut(s) 1070
MvnI CGCG 2 cut(s) 38, 137
NcoI CCATGG 1 cut(s) 771
NdeII GATC 2 cut(s) 775, 1165
NheI GCTAGC 1 cut(s) 553
NlaIII CATG 6 cut(s) 625, 737, 775, 797, 823, 1153
NlaIV GGNNCC 2 cut(s) 777, 1215
NmuCI GTSAC 1 cut(s) 545
NspI RCATGY 2 cut(s) 625, 737
PaqCI CACCTGC 1 cut(s) 1214
PcsI WCGNNNNNNNCGW 1 cut(s) 217
PfeI GAWTC 1 cut(s) 761
PfoI TCCNGGA 1 cut(s) 1068
PkrI GCNGC 6 cut(s) 40, 247, 256, 1012, 1099, 1129
PleI GAGTC 1 cut(s) 1143
PpsI GAGTC 1 cut(s) 1143
PshBI ATTAAT 1 cut(s) 669
Psp6I CCWGG 1 cut(s) 1068
PspGI CCWGG 1 cut(s) 1068
PspN4I GGNNCC 2 cut(s) 777, 1215
PstI CTGCAG 2 cut(s) 250, 1285
PstNI CAGNNNCTG 1 cut(s) 896
PsuI RGATCY 1 cut(s) 775
PvuII CAGCTG 2 cut(s) 263, 646
RsaI GTAC 5 cut(s) 214, 365, 503, 642, 806
RsaNI GTAC 5 cut(s) 213, 364, 502, 641, 805
SaqAI TTAA 5 cut(s) 303, 656, 669, 1103, 1259
SatI GCNGC 6 cut(s) 39, 246, 255, 1011, 1098, 1128
Sau3AI GATC 2 cut(s) 775, 1165
ScaI AGTACT 1 cut(s) 503
SchI GAGTC 1 cut(s) 1144
ScrFI CCNGG 1 cut(s) 1070
SduI GDGCHC 2 cut(s) 132, 733
SfaNI GCATC 3 cut(s) 460, 1219, 1264
SfcI CTRYAG 2 cut(s) 246, 1281
SmlI CTYRAG 2 cut(s) 142, 561
SmoI CTYRAG 2 cut(s) 142, 561
Sse9I AATT 7 cut(s) 114, 300, 401, 666, 698, 915, 1240
SsiI CCGC 2 cut(s) 38, 254
SspMI CTAG 3 cut(s) 554, 765, 800
StyD4I CCNGG 1 cut(s) 1068
StyI CCWWGG 2 cut(s) 771, 926
TaaI ACNGT 5 cut(s) 242, 377, 421, 446, 809
TaiI ACGT 3 cut(s) 748, 1007, 1165
TaqI TCGA 4 cut(s) 9, 132, 197, 1266
TasI AATT 7 cut(s) 114, 300, 401, 666, 698, 915, 1240
TatI WGTACW 1 cut(s) 501
TauI GCSGC 2 cut(s) 41, 257
TfiI GAWTC 1 cut(s) 761
Tru1I TTAA 5 cut(s) 303, 656, 669, 1103, 1259
Tru9I TTAA 5 cut(s) 303, 656, 669, 1103, 1259
TscAI CASTG 2 cut(s) 480, 515
TseFI GTSAC 1 cut(s) 545
TseI GCWGC 4 cut(s) 245, 1010, 1097, 1127
Tsp45I GTSAC 1 cut(s) 545
TspDTI ATGAA 7 cut(s) 414, 879, 962, 1166, 1188, 1197, 1305
TspRI CASTG 2 cut(s) 480, 515
VspI ATTAAT 1 cut(s) 669
XagI CCTNNNNNAGG 1 cut(s) 956
XapI RAATTY 2 cut(s) 698, 915
XceI RCATGY 2 cut(s) 625, 737
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XspI CTAG 3 cut(s) 554, 765, 800
ZrmI AGTACT 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.