Prupe.5G216800_v2.0.a1

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001364
Physical Location & Seq
Reverse (-)
16986705 .. 16992168
5464 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G216800.1

Sequence Viewer

Length: 1431 bp
ATGAGCATCGAGCCTTTCAACAAGTTAGTGAAGCTTGTGGGGAGAGCGTTCTACGACGACATTACCAAAGGAGATAATCAGCCCAAGACCGGCCGGAGCGACAATAGGGGAATCGCCGTGGTGGTGCTCGATGCCCTCTCTAGACGACAATGGGTCAGAGAAGAGGATTTGGCAAAGGACTTGAAACTGCACTCAAAGCAACTTCGTCGTACTTTGCGATTTTTTGAGGAGGAAAAACTGGTCACCAGAGATCATAGGAGAGAGACAGCTAAGGGTGCGAAGATGCATAATGCTGCTGTTGCTGCCACGATTGATGGGCTACCTGCAAAAGAAGGGGAAGAGAAAGTTAAGATGCACACTCACTCTTATTGCTGTCTGGACTATGCGCAGATATCCGATGTGGTCAGGTACAGAGTGCAGCGTATGAAGAAAAAACTGAAAGATGAATTGGAGGACAAGAACACTGTTCAGGAATATGTATGCCCTAACTGTGGGAAAAGATACAACGCTTTAGATGCATTGCAACTGGTTTCTATGGAGGATGAGTACTTCCACTGTGAAAGTTGTAATGGGGAACTTGTGGCTGAGAGTGACAAGCTAGCTTCACAAGAAGTCGGAGATGGAGATGACAATGCGAGGAGGCGACGGCGTGAAAAATTGAAAGACTTGCTTCAAAAGATGGAGGCACAACTAAAGCCTATATATGATCAACTTAATAGAGTTAAAGACTTACCTGCTCCTGAATTTGGAGGTCTCCAAGCTTGGGAAGCGCGAGCAAGTGCAGCTAAACGTGCAGAGAATGGTGAATCTAATGGCATGGATCCCTCCAAATCTTCTCATGGGTTGGGTTATGGTGGAACACCAATGCCCTTTCTTGGAGATACAAAGGTTGAAGTTGACTTTTCTGGGGTTGAAGGCAAGGGGAAGGATATCAAATCTGAAGCTGATGGTGCAGGCCTAAAAGTTTTGCCACCTTGGATGATCAAGCAAGGGATGAACCTTACAAAGGAACAACGCGGAGAGGTCAAGCAGGAGGCAAAGATGGATGGCAGTGGCAGTTTAGCAGCTCCAGAATACTCAGAGGACAAAAAGTTTAATACTGAAAACAATGAGAAGAATTTACAGGATGAGTACTTGAAAGCTTATTACGCTGCTTTAATAAAAAGACAAGAAGAAGAAGAAGCAAAGAAAGCACAGGAATCCTCAGAAACACGTGTCTCTGATGCTCCTTCCGGAATGTCTCCTAGCCGTCAGGTCGGCATGAAATCAAAGCGTGATGAGGATGAAGGAGATGATAATGTGGACTGGGAAGAGGCTCCAATTACAGGTAATGAAGAAAATTACAAGGTCAATGACTTGAATGTGGAAGCTGATGCTACAGCAGAGGCAGAGGCAGAGGATGAGGATGATATAGACTGGGAGGAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

477

Amino Acids

53.59

Weight (kDa)

5.02

Isoelectric Point (pI)

56.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 387
Acc36I ACCTGC 2 cut(s) 331, 742
AccII CGCG 2 cut(s) 772, 1017
AccIII TCCGGA 1 cut(s) 1232
AciI CCGC 1 cut(s) 1017
AclWI GGATC 2 cut(s) 815, 828
AcoI YGGCCR 1 cut(s) 91
AcsI RAATTY 2 cut(s) 743, 1117
AcuI CTGAAG 1 cut(s) 960
AcvI CACGTG 1 cut(s) 1214
AfaI GTAC 4 cut(s) 211, 410, 548, 1133
AfiI CCNNNNNNNGG 7 cut(s) 89, 491, 746, 763, 875, 1006, 1325
AflIII ACRYGT 2 cut(s) 1211, 1213
AgsI TTSAA 8 cut(s) 19, 184, 661, 674, 893, 914, 1138, 1360
AhdI GACNNNNNGTC 1 cut(s) 152
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 4 cut(s) 257, 758, 1222, 1245
AlwI GGATC 2 cut(s) 815, 828
Aor13HI TCCGGA 1 cut(s) 1232
AoxI GGCC 2 cut(s) 91, 955
ApeKI GCWGC 6 cut(s) 293, 302, 418, 782, 1064, 1151
ApoI RAATTY 2 cut(s) 743, 1117
AspLEI GCGC 2 cut(s) 388, 772
AsuHPI GGTGA 2 cut(s) 235, 815
AsuNHI GCTAGC 1 cut(s) 598
BaeI ACNNNNGTAYC 2 cut(s) 873, 906
BamHI GGATCC 1 cut(s) 820
BbrPI CACGTG 1 cut(s) 1214
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 6 cut(s) 280, 289, 430, 794, 1076, 1138
BccI CCATC 6 cut(s) 308, 614, 673, 941, 1036, 1040
BceAI ACGGC 3 cut(s) 101, 662, 1233
BcgI CGANNNNNNTGC 2 cut(s) 188, 222
BclI TGATCA 2 cut(s) 706, 981
BcoDI GTCTC 4 cut(s) 257, 758, 1222, 1245
BfaI CTAG 3 cut(s) 141, 599, 1245
BfmI CTRYAG 1 cut(s) 1377
BfuAI ACCTGC 2 cut(s) 331, 742
BisI GCNGC 6 cut(s) 294, 303, 419, 783, 1065, 1152
BlsI GCNGC 6 cut(s) 295, 304, 420, 784, 1066, 1153
BmcAI AGTACT 2 cut(s) 548, 1133
BmeRI GACNNNNNGTC 1 cut(s) 152
BmiI GGNNCC 2 cut(s) 822, 1317
BmrI ACTGGG 2 cut(s) 1315, 1426
BmsI GCATC 7 cut(s) 15, 121, 273, 342, 505, 1213, 1363
BmtI GCTAGC 1 cut(s) 602
BmuI ACTGGG 2 cut(s) 1315, 1426
BpmI CTGGAG 1 cut(s) 1053
Bpu10I CCTNAGC 1 cut(s) 270
BsaAI YACGTR 1 cut(s) 1214
BsaI GGTCTC 1 cut(s) 758
BsaJI CCNNGG 2 cut(s) 117, 974
BsaWI WCCGGW 1 cut(s) 1232
BsaXI ACNNNNNCTCC 2 cut(s) 530, 560
Bsc4I CCNNNNNNNGG 7 cut(s) 89, 491, 746, 763, 875, 1006, 1325
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 4 cut(s) 243, 531, 1310, 1421
Bse3DI GCAATG 1 cut(s) 518
BseAI TCCGGA 1 cut(s) 1232
BseDI CCNNGG 2 cut(s) 117, 974
BseGI GGATG 8 cut(s) 547, 984, 999, 1051, 1132, 1288, 1405, 1411
BseLI CCNNNNNNNGG 7 cut(s) 89, 491, 746, 763, 875, 1006, 1325
BseMI GCAATG 1 cut(s) 518
BseMII CTCAG 3 cut(s) 576, 1092, 1218
BseNI ACTGG 4 cut(s) 243, 531, 1310, 1421
BseRI GAGGAG 2 cut(s) 242, 652
BseX3I CGGCCG 1 cut(s) 91
BseXI GCAGC 6 cut(s) 280, 289, 430, 794, 1076, 1138
BsgI GTGCAG 5 cut(s) 173, 437, 801, 813, 972
Bsh1236I CGCG 2 cut(s) 772, 1017
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 2 cut(s) 93, 957
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 129
BsiSI CCGG 3 cut(s) 90, 94, 1233
BslI CCNNNNNNNGG 7 cut(s) 89, 491, 746, 763, 875, 1006, 1325
BsmAI GTCTC 4 cut(s) 257, 758, 1222, 1245
BsnI GGCC 2 cut(s) 93, 957
Bso31I GGTCTC 1 cut(s) 758
Bsp1286I GDGCHC 1 cut(s) 129
Bsp13I TCCGGA 1 cut(s) 1232
Bsp143I GATC 4 cut(s) 250, 706, 820, 981
BspACI CCGC 1 cut(s) 1017
BspANI GGCC 2 cut(s) 93, 957
BspCNI CTCAG 3 cut(s) 577, 1091, 1217
BspEI TCCGGA 1 cut(s) 1232
BspFNI CGCG 2 cut(s) 772, 1017
BspLI GGNNCC 2 cut(s) 822, 1317
BspMI ACCTGC 2 cut(s) 331, 742
BspOI GCTAGC 1 cut(s) 602
BspPI GGATC 2 cut(s) 815, 828
BspTNI GGTCTC 1 cut(s) 758
BsrDI GCAATG 1 cut(s) 518
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 4 cut(s) 243, 531, 1310, 1421
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 117, 974
BssMI GATC 4 cut(s) 250, 706, 820, 981
BssT1I CCWWGG 1 cut(s) 974
Bst4CI ACNGT 3 cut(s) 466, 491, 557
Bst6I CTCTTC 3 cut(s) 156, 333, 1305
BstBAI YACGTR 1 cut(s) 1214
BstC8I GCNNGC 3 cut(s) 600, 774, 955
BstDEI CTNAG 4 cut(s) 270, 585, 1078, 1204
BstDSI CCRYGG 1 cut(s) 117
BstEII GGTNACC 1 cut(s) 241
BstENI CCTNNNNNAGG 1 cut(s) 1004
BstF5I GGATG 8 cut(s) 547, 984, 999, 1051, 1132, 1288, 1405, 1411
BstFNI CGCG 2 cut(s) 772, 1017
BstHHI GCGC 2 cut(s) 388, 772
BstKTI GATC 4 cut(s) 253, 709, 823, 984
BstMAI GTCTC 4 cut(s) 257, 758, 1222, 1245
BstMBI GATC 4 cut(s) 250, 706, 820, 981
BstMCI CGRYCG 1 cut(s) 94
BstPI GGTNACC 1 cut(s) 241
BstSFI CTRYAG 1 cut(s) 1377
BstUI CGCG 2 cut(s) 772, 1017
BstV1I GCAGC 6 cut(s) 280, 289, 430, 794, 1076, 1138
BstX2I RGATCY 1 cut(s) 820
BstYI RGATCY 1 cut(s) 820
BstZI CGGCCG 1 cut(s) 91
BsuRI GGCC 2 cut(s) 93, 957
BtgI CCRYGG 1 cut(s) 117
BtsCI GGATG 8 cut(s) 547, 984, 999, 1051, 1132, 1288, 1405, 1411
BtsI GCAGTG 1 cut(s) 1057
BtsIMutI CAGTG 3 cut(s) 462, 553, 1057
BveI ACCTGC 2 cut(s) 331, 742
Cac8I GCNNGC 3 cut(s) 600, 774, 955
CfoI GCGC 2 cut(s) 388, 772
Cfr10I RCCGGY 1 cut(s) 89
Csp6I GTAC 4 cut(s) 210, 409, 547, 1132
CviAII CATG 3 cut(s) 817, 839, 1261
CviQI GTAC 4 cut(s) 210, 409, 547, 1132
DdeI CTNAG 4 cut(s) 270, 585, 1078, 1204
DpnI GATC 4 cut(s) 252, 708, 822, 983
DpnII GATC 4 cut(s) 250, 706, 820, 981
DriI GACNNNNNGTC 1 cut(s) 152
EaeI YGGCCR 1 cut(s) 91
EagI CGGCCG 1 cut(s) 91
Eam1104I CTCTTC 3 cut(s) 156, 333, 1305
Eam1105I GACNNNNNGTC 1 cut(s) 152
EarI CTCTTC 3 cut(s) 156, 333, 1305
EclXI CGGCCG 1 cut(s) 91
Eco130I CCWWGG 1 cut(s) 974
Eco147I AGGCCT 1 cut(s) 957
Eco31I GGTCTC 1 cut(s) 758
Eco32I GATATC 2 cut(s) 393, 931
Eco52I CGGCCG 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 960
Eco72I CACGTG 1 cut(s) 1214
Eco91I GGTNACC 1 cut(s) 241
EcoNI CCTNNNNNAGG 1 cut(s) 1004
EcoO65I GGTNACC 1 cut(s) 241
EcoRV GATATC 2 cut(s) 393, 931
EcoT14I CCWWGG 1 cut(s) 974
EcoT22I ATGCAT 2 cut(s) 288, 520
ErhI CCWWGG 1 cut(s) 974
FaeI CATG 3 cut(s) 820, 842, 1264
FalI AAGNNNNNCTT 2 cut(s) 654, 686
FatI CATG 3 cut(s) 816, 838, 1260
FbaI TGATCA 2 cut(s) 706, 981
Fnu4HI GCNGC 6 cut(s) 294, 303, 419, 783, 1065, 1152
FokI GGATG 8 cut(s) 554, 991, 1006, 1058, 1139, 1295, 1412, 1418
Fsp4HI GCNGC 6 cut(s) 294, 303, 419, 783, 1065, 1152
FspBI CTAG 3 cut(s) 141, 599, 1245
FspI TGCGCA 1 cut(s) 387
GlaI GCGC 2 cut(s) 387, 771
GluI GCNGC 6 cut(s) 294, 303, 419, 783, 1065, 1152
GsuI CTGGAG 1 cut(s) 1053
HaeIII GGCC 2 cut(s) 93, 957
HapII CCGG 3 cut(s) 90, 94, 1233
HhaI GCGC 2 cut(s) 388, 772
Hin1II CATG 3 cut(s) 820, 842, 1264
Hin6I GCGC 2 cut(s) 386, 770
HinP1I GCGC 2 cut(s) 386, 770
HincII GTYRAC 1 cut(s) 898
HindII GTYRAC 1 cut(s) 898
HindIII AAGCTT 3 cut(s) 32, 759, 1140
HinfI GANTC 3 cut(s) 111, 806, 1199
HpaII CCGG 3 cut(s) 90, 94, 1233
HphI GGTGA 2 cut(s) 235, 815
Hpy166II GTNNAC 2 cut(s) 898, 1303
Hpy188I TCNGA 7 cut(s) 158, 397, 617, 940, 1081, 1207, 1222
Hpy188III TCNNGA 6 cut(s) 141, 377, 470, 740, 1070, 1233
Hpy8I GTNNAC 2 cut(s) 898, 1303
Hpy99I CGWCG 3 cut(s) 59, 210, 648
HpyAV CCTTC 6 cut(s) 326, 908, 919, 1239, 1280, 1418
HpyCH4III ACNGT 3 cut(s) 466, 491, 557
HpyCH4IV ACGT 2 cut(s) 790, 1213
HpyF3I CTNAG 4 cut(s) 270, 585, 1078, 1204
HpySE526I ACGT 2 cut(s) 790, 1213
Hsp92II CATG 3 cut(s) 820, 842, 1264
HspAI GCGC 2 cut(s) 386, 770
Kpn2I TCCGGA 1 cut(s) 1232
Ksp22I TGATCA 2 cut(s) 706, 981
Kzo9I GATC 4 cut(s) 250, 706, 820, 981
LmnI GCTCC 5 cut(s) 96, 742, 1072, 1231, 1321
Lsp1109I GCAGC 6 cut(s) 280, 289, 430, 794, 1076, 1138
LweI GCATC 7 cut(s) 15, 121, 273, 342, 505, 1213, 1363
MaeI CTAG 3 cut(s) 141, 599, 1245
MaeII ACGT 2 cut(s) 790, 1213
MaeIII GTNAC 2 cut(s) 241, 590
MalI GATC 4 cut(s) 252, 708, 822, 983
MboI GATC 4 cut(s) 250, 706, 820, 981
MflI RGATCY 1 cut(s) 820
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 6 cut(s) 446, 656, 743, 1117, 1320, 1339
MmeI TCCRAC 1 cut(s) 595
Mph1103I ATGCAT 2 cut(s) 288, 520
MroI TCCGGA 1 cut(s) 1232
MseI TTAA 5 cut(s) 348, 714, 723, 1095, 1157
MspI CCGG 3 cut(s) 90, 94, 1233
MvnI CGCG 2 cut(s) 772, 1017
NdeII GATC 4 cut(s) 250, 706, 820, 981
NheI GCTAGC 1 cut(s) 598
NlaIII CATG 3 cut(s) 820, 842, 1264
NlaIV GGNNCC 2 cut(s) 822, 1317
NmuCI GTSAC 2 cut(s) 241, 590
NsbI TGCGCA 1 cut(s) 387
NsiI ATGCAT 2 cut(s) 288, 520
PceI AGGCCT 1 cut(s) 957
PcsI WCGNNNNNNNCGW 1 cut(s) 214
PfeI GAWTC 3 cut(s) 111, 806, 1199
PkrI GCNGC 6 cut(s) 295, 304, 420, 784, 1066, 1153
PmaCI CACGTG 1 cut(s) 1214
PmlI CACGTG 1 cut(s) 1214
Ppu21I YACGTR 1 cut(s) 1214
PspCI CACGTG 1 cut(s) 1214
PspEI GGTNACC 1 cut(s) 241
PspN4I GGNNCC 2 cut(s) 822, 1317
PsuI RGATCY 1 cut(s) 820
RsaI GTAC 4 cut(s) 211, 410, 548, 1133
RsaNI GTAC 4 cut(s) 210, 409, 547, 1132
SaqAI TTAA 5 cut(s) 348, 714, 723, 1095, 1157
SatI GCNGC 6 cut(s) 294, 303, 419, 783, 1065, 1152
Sau3AI GATC 4 cut(s) 250, 706, 820, 981
ScaI AGTACT 2 cut(s) 548, 1133
SduI GDGCHC 1 cut(s) 129
SfaNI GCATC 7 cut(s) 15, 121, 273, 342, 505, 1213, 1363
SfcI CTRYAG 1 cut(s) 1377
Sse9I AATT 6 cut(s) 446, 656, 743, 1117, 1320, 1339
SseBI AGGCCT 1 cut(s) 957
SsiI CCGC 1 cut(s) 1017
SspMI CTAG 3 cut(s) 141, 599, 1245
StuI AGGCCT 1 cut(s) 957
StyI CCWWGG 1 cut(s) 974
TaaI ACNGT 3 cut(s) 466, 491, 557
TaiI ACGT 2 cut(s) 793, 1216
TaqI TCGA 2 cut(s) 9, 129
TasI AATT 6 cut(s) 446, 656, 743, 1117, 1320, 1339
TatI WGTACW 2 cut(s) 546, 1131
TfiI GAWTC 3 cut(s) 111, 806, 1199
Tru1I TTAA 5 cut(s) 348, 714, 723, 1095, 1157
Tru9I TTAA 5 cut(s) 348, 714, 723, 1095, 1157
TscAI CASTG 3 cut(s) 469, 560, 1057
TseFI GTSAC 2 cut(s) 241, 590
TseI GCWGC 6 cut(s) 293, 302, 418, 782, 1064, 1151
Tsp45I GTSAC 2 cut(s) 241, 590
TspDTI ATGAA 6 cut(s) 440, 459, 1010, 1277, 1299, 1347
TspRI CASTG 3 cut(s) 469, 560, 1057
XagI CCTNNNNNAGG 1 cut(s) 1004
XapI RAATTY 2 cut(s) 743, 1117
XbaI TCTAGA 1 cut(s) 140
XspI CTAG 3 cut(s) 141, 599, 1245
ZrmI AGTACT 2 cut(s) 548, 1133
Zsp2I ATGCAT 2 cut(s) 288, 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.